ALLCELLS
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2015-2016 PRODUCT CATALOG
YOUR RELIABLE
SOURCE OF
PRIMARY CELLS
BIO
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RV
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S
®
HE
MA
TO
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®
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BIOSERVICES
PRODUCT CATALOG 2015-2016
1
1
Thank you for trusting us with your research!
We appreciate the opportunity to serve as your
hematology/immunology research partner...and we've
been proud to stand by you for the past 18 years.
-The AllCells Team
ALLCELLS®
AllCells, LLC
1301 Harbor Bay Park way, Suite 200
Alameda, California 94502
Phone:
1-888-535-3444, 510 -521-2600
AllCells,
LLC
Toll Free Phone: 1-888-535-3444
Fa x: 510 -521-7600
Toll Free Phone: 1-888-535-3444
ALLCELLS
12-2015
w w w.allcells.com
Cover Image:
Various images of AllCells team members, our products, and facilities located in Alameda, CA.
www.allcells.com
www.allcells.com
For In Vitro Research Use
For In Vitro Research Use Only
Only
2
A Message to Our Valued Customers:
Dear Valued Customer,
Welcome to AllCells’ new 2015-16 Product Catalog. It is my sincere hope you find our new catalog to be an
informative, useful resource in helping plan your hematology & immunology primary
cell-based research.
AllCells was founded upon a commitment to service the primary cell needs of life science researchers
worldwide, who depend upon obtaining just the right cell phenotype, human donor profile, tissue cellularity,
clinical report data, and other essentials for enabling cell-based research. In addition to standard catalog
items and Bioservices offering, our aim at AllCells is to accommodate your unique research project
requirements. Our core capabilities span custom hematologic tissue procurement through AllCells’ clinical
division, LeukoLab, and established clinical (diseased tissue) collection network, custom cell isolation
manufacturing, cell biology characterization, and even custom delivery -- when cells simply must arrive at
your lab on that special day. We’re pleased to announce that AllCells now offers weekend Whole Bone
Marrow, and Leuko Pak collections as customized products, enabling Monday morning delivery in most cases.
Providing service excellence is fundamental to AllCells culture, and we invite you to challenge our team with
your customized primary cell project requirements!
Thanks to your ongoing support, AllCells successfully completed the expansion, relocation, and consolidation
of its operations to Alameda, CA. We dramatically increased our production capacity to handle your large
primary cell projects in 2015, and beyond. The contiguous placement of our clinical division, LeukoLab, with
cell manufacturing, packaging & shipping, and administrative functions now provides for an unprecedented,
optimal work-flow, vital for consistently delivering a high level of quality assurance.
More than ever, we’re eager to learn how AllCells can serve as a vital partner in helping fulfill your research
project goals & objectives. We encourage you to contact any of our Sales Representatives, Customer Service,
or authorized Distributors worldwide, to discuss how we can add value to your research efforts.
We are thankful for your business and look forward to continuing to earn your trust.
Yours truly,
Jay Tong
Jay Tong, M.D.
President & CEO
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
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3
TABLE OF CONTENTS
Helpful Information
Eosinophils51
Ordering/Delivery/Storage Information5-8
Getting Support9
General Product Information10-11
Terms & Conditions
12-14
Tissue Collection Facility
Neutrophils52
Red Blood Cells/Mature Erythrocytes 53
Platelets 54
Monocytes
CD14+ Monocytes55
Dendritic Cells (Monocyte-Derived)
56
Macrophages57
Background Information15-16
Mobilization, Donor Criteria, Capabilities
17
Lead Times, Matched Sample Collection
18
Mobilized Peripheral Blood
Background Information58
Bioservices
Leuko Pak (Apheresis Collection)
Background Information19-21
MNC60
Immunoassays22
CD34-Depleted MNC61
Hematopoietic Assays23
Stem/Progenitor Cells
Flow Cytometry Services24
CD34+62
Tissue Culture Services25
CD133+ 63
Molecular Services26
Myeloid Cells
Custom HLA-Typing27
Human Primary Cells & Tissue
Bone Marrow
59
Mononuclear Cells
Monocytes (CD14+)64
Background Information28-29
Background Information65
Non-Diseased Custom Products
Aspirates—Bone Marrow (Whole)
30
66
Mononuclear Cells (MNC) Normal Peripheral Blood
MNC 67
Background Information32
Leuko Pak (Apheresis Collection)
33
Whole Blood34-35
Plasma36
Mononuclear Cells (MNC)37
Stem/Progenitor Cells (CD34+)38
Lymphoid Cells
B Cells(CD19+, CD19+/IgD+, Memory)
39-40
T Cells (CD3+ Pan, CD4+ Helper, CD4+/CD25+,41-47
CD4+/CD45RA+/CD25- Naive Helper,
CD34-Depleted MNC68
Stem/Progenitor Cells
CD34 + 69
Myeloid Cells Early Erythroid Progenitor Cells (CD36+) Mesenchymal Cells
70
Endothelial Cells (CD105+) 71
Mesenchymal Stem Cells
72
Cord Blood
CD4+/CD45RO+ Memory, CD8+ Cytotoxic,
Background Information73
CD8 /CD45RA Naive Cytotoxic)
Plasma74
+
+
Dendritic Cells (BDCA4+ Lymphoid/Plasmacytoid)
48
Natural Killer Cells (CD56 )49
+
Mononuclear Cells (MNC) MNC75
CD34-Depleted MNC76
Myeloid Cells
Basophils50
ALLCELLS
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TABLE OF CONTENTS
Stem/Progenitor Cells
CD34 +77
Background Information121
Myeloid Cells
Early Erythroid Progenitor Cells (CD36 +)
78
Monocytes (CD14 )79
+
Lymphoid Cells
B Cells (CD19+)80
T Cells (CD3+ Pan, CD4+ Helper, CD8+ Cytotoxic, 81-84
CD4+/CD45RA+ Naive)
Animal Primary Cells & Tissue
Natural Killer Cells (CD56 +)85
Non-Hematopoietic Stem Cells & Tissue
Background Information86
Non-Human Primate Cells122
Cell Culture Products
Background Information123
Basic Cell Culture & Cryopreservation Media
124
Defined Cell Culture Media
125
Cytokines
126-128
Technical Notes
How to Count Cells
130
How to Thaw Frozen Cells
131
Diseased Cells & Tissue
How to Freeze Cell Products
132-133
How to Purify Bone Marrow MNC
134-135
Background Information88-89
How to Count Leuko Pak
136
Diseased Custom Products90
How to Deplete Red Blood Cells
137
Leukemia
How to Passage & Subculture Cells
138-139
How to Isolate MNC using a Ficoll Gradient
140
Dental Pulp Cells87
Background Information
91-92
Acute Lymphoid Leukemia (ALL)
93-94
Acute Myeloid Leukemia (AML)
95-96
Chronic Lymphoid Leukemia (CLL)
97-98
Chronic Myeloid Leukemia (CML)
99-100
Multiple Myeloma (MM)
101-103
Myelodysplastic Syndrome (MDS)
104-105
Non-Hodgkin's Lymphoma
106-107
Index
Alphabetical Index141-143
Notes144
Quick Reference Guide145-146
Autoimmune
Background Information
108
Grave's Thyroid
109
Hashimoto's Thyroiditis
110
Multiple Sclerosis (MS)
111
Osteoarthritis (OA)
112
Psoriasis (PSO)
113
Rheumatoid Arthritis (RA)
114
Systemic Lupus Erythematosus
115
Type I Diabetes (DIA1)
116
Type II Diabetes (DIA2)
117
Autoimmune: Inflammatory Bowel Disease
Background Information118
Crohn's Disease119
Ulcerative Colitis120
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
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5
Ordering, Delivery & Storage Information
Ordering Information
Contact Information
Phone
(510) 521-2600
Toll-Free
(888) 535-3444
Fax
(510) 521-7600
Email
sales@allcells.com
Online
allcells.com
Address
1301 Harbor Bay Parkway, Suite 200
Alameda, CA 94502
Office Hours
Monday through Friday
8:30am to 5:30pm (Pacific Time)
Order, price, and delivery date are confirmed after AllCells receives your purchase
order or credit card information. Please provide us with your email address so we
can notify you about the shipment status. All International orders must be paid in full
prior to shipment.
Lead Times*
Product Format
Fresh
Normal Peripheral Blood
Mobilized Peripheral Blood
Leuko Paks and
Leuko Paks and
Leuko Pak derivatives:
Leuko Pak derivatives:
2 weeks
4 weeks
Frozen
Whole Blood
Bone Marrow
Cord Blood
1-2 weeks
2 weeks
2 weeks
1-2 business days
*Lead times apply to fresh healthy donor products and frozen in stock items only. Diseased tissue and cells are limited by stock
availability. Lead times may vary due to the nature of the disease and sensitivity of patients. Custom product lead times may vary. See
Custom Product section on pg. 30 and 90.
Delivery Information
Media Shipment & Delivery Information
Media
Package
Examples
Stimulatory Supplement
Packaged in dry ice at
shipment.
MSC Stimulatory Supplement
Basal Medium
Packaged with a cold gel pack
at shipment.
MSC Basal Medium
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
Courier
Packages are shipped using FedEx
Priority Overnight service with a
delivery commitment of 10:30am local
time. Other delivery services, including
same day delivery, are available upon
request.
For In Vitro Research Use Only
6
Ordering, Delivery & Storage Information
Cell Type
Fresh
Frozen*/Lysates
Whole tissue
Transferred to a conical tube containing
DPBS buffer. Shipped at room
temperature.
Lysates are packaged in dry ice at
shipment.
Plasma/
Platelets
Shipped at room temperature.
Snap Frozen. Packaged in dry ice at
shipment.
Whole Blood
RBC
Mononuclear Cells
(MNC)
Drawn into a blood bag,
syringe(s), or blood tube(s). Shipped at
room temperature.
Transferred to a 50mL conical tube
containing fresh RBCs and shipped in
donor plasma. Packaged with a cold
gel pack.
Transferred to a 50mL conical tube
with shipping media (2mM EDTA).
Packaged with a cold gel pack.
Purified Cells
Transferred to a small centrifuge tube
containing shipping media (IMDM +
10% FBS + 2mM EDTA). Packaged
with a cold gel pack.
Leuko Pak
(non-mobilized +
mobilized)
One-liter blood bag containing
peripheral blood cell MNCs and
autologous plasma. Shipped at room
temperature. Two-day collections are
shipped out separately.
Cultured Cells
Cells are cultured and transported in
flasks or an OptiCell plate that has two
50cm2 gas permeable, optically clear
growth membranes.**
Frozen MNC are stored in a solution
of 50% IMDM, 40% FBS, 10%
DMSO (0.2 µm filtered) in the vapor
phase of liquid nitrogen storage until
ready for shipment. Packaged in dry
ice at shipment. (Exception: BM-MNC
are stored in standard CryoStor
media.)
Frozen cells are stored in a solution of
50% IMDM, 40% FBS, 10% DMSO
(0.2 µm filtered) in liquid nitrogen
storage until ready for shipment.
Packaged in dry ice at shipment.
Stored in a 500μL microtube with 20
to 100 μL DEPC water. Packaged in
dry ice at shipment.
cDNA
Aliquoted at a base concentration of
one PCR reaction/2μL DEPC-H2O.
Packaged in dry ice at shipment.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Packages are shipped using
FedEx Priority Overnight
service with a delivery
commitment of 10:30am
local time. Other delivery
services, including same day
delivery (limited to Bay Area or
California), are available upon
request.
Frozen cells are stored in a solution of
50% IMDM, 40% FBS, 10% DMSO
(0.2 µm filtered) in liquid nitrogen
storage until ready for shipment.
Packaged in dry ice at shipment.
RNA
Courier
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Ordering, Delivery & Storage Information
Frozen Cell Storage Conditions
For short-term storage (<1 month), store AllCells products in –80˚C
freezer.
For long-term storage (>1 month), store in the vapor phase of liquid
nitrogen storage tank.
handling LN2 and when handling cryovials and other sealed
containers that have been stored in LN2.
Safety Precautions
Storage Precautions
AllCells uses cryovials that are designed for vapor phase liquid
nitrogen storage.
** WARNING: Do not store AllCells frozen cell products in the
liquid phase of liquid nitrogen (LN2). Liquid can enter closed
screw top cryovials, which then have the potential to explode
when removed from storage. **
If long-term storage in the LN2 liquid phase is required, do not
use the cryovials provided by AllCells.
If AllCells products are stored in liquid phase of LN2, at -20˚C, or
-80˚C for >1 month will void any warranty of the product.
Laboratory personnel should use extreme caution when storing
samples in LN2. LN2 storage consists of a liquid phase and a gaseous.
If cryovials are immersed in the liquid phase, LN2 can enter the closed
screw-top cryovials during storage. The cryovial may then explode
when it is removed from storage due to the vaporization and expansion
(700x expansion ratio) of the liquid nitrogen inside the cryovial.
Use either:
• Manufacturer-approved cryovials, specifically designed for
liquid phase storage.
• Gaseous phase-approved screw-top cryovials that are then
hermetically sealed in an outer protective envelope designed
for use in LN2.
The handling of cryovials inside of Biological Safety Cabinets or
Chemical Hoods (with the sash lowered) will further reduce the risk
of injury from explosions caused by excess pressure within the vial.
Health Hazards of Liquid Nitrogen
Limited Warranty:
Liquid nitrogen has a 700x expansion ratio, which may cause physical
hazards and injuries from the explosion of cryovials, containers,
equipment, or other devices. Extensive tissue damage or burns can
result from exposure to LN2 or cold nitrogen vapors. Asphyxiation
may result from the displacement of oxygen in the air with nitrogen to
levels where there is insufficient oxygen. Inhalation of oxygen deficient
air can cause dizziness, nausea, vomiting, loss of consciousness, and
death.
AllCells warranty of frozen cell products for a period of 1 year form
the date of receipt if stored properly.
References
Cryovial: Simport T310 Cryovial ®
Personal Protective Equipment
For more information regarding cryovials, please visit Simport
http://www.simport.com/products/cryogenics/cryogenic-vials/
t310-cryovial.html
The following personal protective equipment is encouraged when
handling or using LN2:
For more information on AllCells, LLC products, please contact an
AllCells Customer Service Representative at info@allcells.com.
Cryo gloves /Waterproof thermal insulated gloves
• Hands should be protected with waterproof thermal
insulated gloves that can be quickly removed if LN2 is spilled
on them. Cryo gloves are not intended for submersing hands
into LN2.
CAUTION
Lab coats
• Body must be protected with pants, lab coats, and closed-toe
shoes.
Safety goggles
• Eyes are sensitive to the extreme cold of LN2 and its vapors.
Over-pressurization may result in the explosion of improperly
stored cryovials. Chemical splash goggles should be used when
ALLCELLS
Toll Free Phone: 1-888-535-3444
The products purchased from AllCells, LLC are “For In Vitro
Research Use Only.” In compliance with U.S. Food and Drug
Administration (FDA) Regulations, these products are intended
for use neither in clinical diagnostic procedures nor in
therapeutic procedures.
These products must be:
1. Treated as potentially contaminated biological specimens even
if available serological reports are negative.
2. Handled by establishing or following appropriate safety control
procedures to ensure the safety of using these products.
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Ordering, Delivery & Storage Information
What additional information will accompany my AllCells Products?
Cell product packages contain:
A Certificate of Analysis
The Certificate of Analysis lists the following:
• Catalog number
• Catalog description
• Donor information (as provided)
• Viral results (HIV, HBV, HCV)
In addition, each purified cell product package contains:
Flow Cytometric Analysis
The figure below is a sample Flow Cytometric Analysis of
our CD34+ enriched progenitor cells from human mobilized
peripheral blood. The upper right panel shows the viability
of cells and the lower right panel displays the purity of cells.
The lower left panel represents the control. Samples were
collected on a BD FACSCaliburTM.
Side scatter
Side scatter
If applicable:
• Cell viability
• Cell purity
• Cell count
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
9
Getting Support
Technical Support
How to reach us
At AllCells we are committed to providing the best support
Customer service is a way of life at AllCells and we pride
possible for our customers. We are dedicated to sharing our
ourselves on going 'above and beyond' to answer any support
expertise in the hematology field to compliment our industry-
questions you may have. If you were not able to find an answer
leading products.
to your question on our website please contact us directly.
You may find multiple resources on our
General product information, availability, sales inquiries and
website including:
ordering: sales@allcells.com
Technical notes, product 'How to' guides, For technical questions or order inquiries:
BioTube technical videos:
www.allcells.com/contact-us
www.allcells.com/support/how-to
Facebook: www.facebook.com/allcells
Webinars, support presentations:
www.allcells.com/support/webinars
LinkedIn: www.linkedin.com/company/allcells-llc
Publications, research citing AllCells Twitter: twitter.com/allcells_bio
products and services:
www.allcells.com/publications
Phone: (888) 535-3444, (510) 521-2600
Fax: (510) 521-7600
Product Data Sheets, request Certificate of
Analysis, MSDS, and Clinical Reports
Mailing address:
(diseased products only):
AllCells, LLC
www.allcells.com/info-request
1301 Harbor Bay Parkway, Suite 200
Alameda, CA 94502
Account Manager support:
www.allcells.com/sales
AllCells Shanghai
Shanghai Ju Ke Science Park
Technical support:
www.allcells.com/technical-support
333 Guiping Road
Building 7, 2nd Floor
Xuhui District, 200233
Shanghai, China
Phone: +86-(0)-21-6485-3421
Fax: +86-(0)-21-64885-0144
www.china.allcells.com
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
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GENERAL PRODUCT INFORMATION
STATEMENT OF ETHICAL STANDARDS
We, the staff members of AllCells, LLC, commit ourselves to the following principles, which we recognize are the standards of
ethical behavior and excellence in practice that are fundamental to all activities in biotechnology:
Respect for Human Individuals
We recognize that all humans have inherent dignity and that the welfare, rights, beliefs, perceptions, customs and cultural
heritage of individuals should be taken into account. We treat clients, donors, colleagues, and all others who seek information
about our organization with this respect.
Respect for Animals
Animals have inherent value in their being as well as in their relation to human culture and research. We believe the welfare and
humane treatment of animals should be maintained.
Respect for the Natural Environment
The natural environment is our common heritage and sustains all life. We recognize that the natural environment and the natural
resources of the earth are more than a means to satisfy human ends. The safeguarding of biodiversity, ecosystems,
and the beauty of the natural environment should always be considered for both the present time and in the interest of
future generations.
Respect for the Public Good
We recognize that our individual and group activities occur in the context of social and institutional relationships. The “public
good” is a collective good that should be taken into account along with individual interests. AllCells is committed to ensuring that
this respect is embedded in the way we do business.
Benefit and Harm
We believe human activities have social, economic, and environmental impacts. Human activities should promote good and avoid
harm. At AllCells, LLC, we strive to create a positive impact in these areas and on the scientific research community as a whole.
Justice and Equity
We believe that the benefits and burdens resulting from biotechnological activities should be distributed equitably throughout
society so that no particular group is inequitably advantaged or disadvantaged now or in the future.
Probity
We believe that our activities should be conducted honestly, truthfully, lawfully, impartially, competently, and with considered
regard for transparency of processes.
Accountability
We recognize that individuals and institutions are inherently responsible for their actions and the justification, purpose, and
consequences of any action should be taken into account when determined. The principles noted here provide guidance for the
application of ethical behavior in our work as members of the biotechnology community.
ALLCELLS
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General Product Information
Cell Separation
Cell Formats
Purified primary cells are processed by positive or negative
AllCells provides a wide range of primary hematopoietic and
isolation using immunomagnetic microbead labeling systems.
immunological cells, which are available from numerous sources,
including normal peripheral blood (NPB), G-CSF (neupogen) or
Microbeads are super paramagnetic particles. They are
CXCR4 (mozobil) mobilized peripheral blood (MPB), bone marrow
conjugated to highly specific antibodies. Labeling does not
(BM), and cord blood (CB). Primary hematopoietic and
affect viability or function of the cells. In addition, these
immunological cells have a limited life span in vitro.
particles have no known effect on structure and do not
interfere with subsequent experiments. Microbeads are
Non-human primate cells are available as well.
biodegradable and typically will detach from the cells after a
AllCells human/animal primary cells are available in fresh or
few days of cell culture.
frozen (cryopreserved). Please note that lysate, cell pellet, Total
Positive Selection: Cells of interest are magnetically labeled
RNA, and/or cDNA products may be available by request for
with microbeads and isolated. This is the most direct and
particular tissue/cell types.
specific way to isolate target cells from heterogeneous
Please note that we guarantee >70% viability of frozen cells after
cell suspensions.
thaw and normal range is between 70% to 98%.
We guarantee that positively-selected cells have a purity of
>90% (unless otherwise specified).
Negative Selection: Negative or untouched cell isolation is
the depletion of unwanted cells. Nontarget cells are magnetically labeled and eliminated from the
cell mixture by immunomagnetic separation. The non-labeled,
untouched cell fraction contains the targeted cells.
Untouched cells are less likely to be activated by antibodies.
We guarantee that the cell purity is >85% (unless otherwise
specified).
ALLCELLS
Toll Free Phone: 1-888-535-3444
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Terms & Conditions
General Terms:
1. These outlined conditions regarding sale and delivery from
AllCells, LLC, (“AllCells”), apply to all contracts of supply
between AllCells and our customers.
2. The Customer is deemed to agree to these terms and
conditions of sale and delivery upon placing an order.
3. Any rights awarded to the Customer arising during the
course of business with AllCells are not transferable.
4. Any amendment to the conditions of sale and delivery, or any
recognition of the Customer’s conflicting purchase conditions
must be confirmed in writing by AllCells.
Prices:
1. AllCells’ prices are subject to change without prior notice.
Please call, fax, or e-mail to confirm current pricing with
AllCells.
2. All shipping and handling charges will be reflected on the
invoice upon the delivery of product. Shipping and handling
charges will vary according to the weight of the package
and the shipping options. Please contact AllCells directly for
current shipping and handling prices.
3. AllCells’ prices quoted in the catalog are net prices; sales tax
will be included where applicable. All shipments to California
destinations are subject to California State sales tax unless
AllCells has a resale certificate on file, or product is derived
from human tissue.
Order Policies (Order, Changes, Cancellation, and Rescheduling):
1. Once you have placed your order, AllCells will recruit and
schedule donors for tissue collection according to your
requirements.
2. Fresh product orders which are cancelled or rescheduled
within the Lead Time Period required for each collection will
incur a 10% Cancellation/Rescheduling Fee according to
the Table below. For example, Lead Time Period for Bone
Marrow collection is 2 weeks, therefore if order is cancelled
or rescheduled within 2 weeks from the scheduled collection
time, customer will be charged a 10% Cancellation/
Rescheduling Fee.
3. Orders may not be cancelled within 24 hours of the
scheduled collection time.
Product Format*
Product Type
Fresh
Mobilized Leuko Pak 4 weeks
Frozen
ALLCELLS
Lead Time Period
Leuko Pak
2 weeks
Bone Marrow
2 weeks
Whole Blood
1-2 weeks
All Products
2 business days
Toll Free Phone: 1-888-535-3444
4. For existing orders, any request for change in volume
of fresh product 10 business days prior to scheduled
draw date is not guaranteed, and must be requested
and approved in writing by Customer Service at orders@
allcells.com.
5. AllCells is not responsible for incomplete orders due to
the following conditions beyond our control: (i) Donors
who do not show-up for tissue collection on a scheduled
draw date, (ii) Donors who fail to complete the collection
process due to their inability to tolerate the full tissue
collection procedure, (iii) Donors fail to complete their
drug mobilization injections, and (iv) Donors do not pass
CBC eligibility on day of draw.
6. All direct international orders are subject to the same
Terms & Conditions that are outlined in AllCells basic
policy except for the following: (i) Direct international
orders must be pre-paid. Customers can pre-pay via
bank wire transfer or credit card (Visa, MC, AMEX), (ii)
International orders are shipped using customer’s courier
account only; AllCells is not liable for the package once
it leaves our facilities; AllCells recommends customers
declare the full value of products to their courier in case
of delay or damage; Customer is responsible for filing any
claims with their courier, (iii) All international couriers must
include an after-hours contact name and phone number
for the end user.
7. AllCells is able to reserve vials and donors to meet
specific customer needs. Please contact Customer
Service to receive details regarding policies and fees.
Back-up Donors:
1. Back-up donors are available for an extra fee for fresh
orders involving same-day multiple donor draws, and
when adhering to delivery schedules is essential. If you
decline a back-up donor option, you will be responsible for
accepting delivery, and invoiced for the lower volume of
product that AllCells is able to collect for your order.
2. AllCells will not be responsible for the following
circumstances: (i) Back-up donors cannot be scheduled,
(ii) Back-up donor does not appear on the scheduled
draw date, and (iii) Back-up donor cannot tolerate the full
procedure, and the total volume needed is not collected
for your order.
*Lead times apply to healthy donor products only.
Diseased tissue and cells are limited by stock
availability. Lead times may vary due to the nature
of the disease and sensitivity of patients.
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13
Terms & Conditions
Use of Goods:
Delivery:
1. All orders are shipped via Federal Express Priority
Overnight delivery unless otherwise specified.
2. Estimated delivery times can be specified; however,
estimated delivery times are not legally binding.
3. Same day delivery is available in the San Francisco Bay
Area with prior arrangement with AllCells.
4. All deliveries take place at the customer’s risk. The
transfer of risk occurs on the handing over of goods to the
carrier or shipper. The course of delivery method is at the
discretion of AllCells with exclusion of any and
all liability.
Payment:
1. For established customers, payments must be made
within 30 days of delivery.
2. Invoices not paid within the designated terms may be
subject to a 10% late fee of the initial balance and a 1.5%
per month (18% annum) interest fee.
3. Failure to make payment within 120 days will result
in credit-hold, and may result in legal and collection
proceedings upon expiration of the payment period
without further warning.
4. AllCells accepts only US currency in the form of company
checks, cashier checks, money orders, AMEX, VISA or
MasterCard payments. All funds must be withdrawable
from a US bank account; foreign bank accounts and
foreign currency will not be accepted as appropriate
payment. Please make checks payable to AllCells.
5. For all other customers (including International),
prepayment via wire fund, credit card (VISA, MasterCard
or American Express), or a check in US dollars is required.
6. If AllCells conditions of payment are not met, or if
“justifiable concerns” exist with regard to the Customer’s
ability or willingness to pay, AllCells, LLC is entitled to
deem any contract with the Customer null and void and
demand the return of the delivered goods in the condition
in which they were shipped. The cost of this return of
delivery will be the responsibility of the Customer. In
addition, all outstanding claims the Customer may have
with AllCells, LLC will become due.
7.
8. “Justifiable concerns” referred to above include, but are
not restricted to: initiation of insolvency or bankruptcy
proceedings, deterioration of financial circumstances, and
default of payment for any other reason.
ALLCELLS
Toll Free Phone: 1-888-535-3444
1. All products received from AllCells are intended exclusively
for in vitro use only. These products are not intended
for use on human beings, animals, or for any private use.
Please note that any use of AllCells products for any
purpose other than intended including use for therapeutic
or diagnostic purposes will result in
legal ramifications.
2. AllCells does not accept any liability for the improper use
of our products. We deliver our products only to research
and teaching institutions, commercial enterprises, and
public researchers. Any signs of improper use of AllCells
products by a customer will result in refusal of delivery and
termination of any contract associated with
this customer.
3. AllCells opposes any research in violation of any ethical,
legal, or governmental standards within the United States
and the import countries of our customers. We do not
accept any liability for such research or use by
our customers.
4. AllCells products are not permitted for resale or as a
source for deriving other cell processing without written
authorization from AllCells.
Liability:
1. AllCells represents and warrants that it has (1) obtained
ownership rights with respect to products and that such
products were provided to AllCells with every donor’s
informed consent and in compliance with all applicable laws
and regulations, (2) obtained all necessary and appropriate
releases from the donor wherein there are no restrictions
on use of products that would prohibit customers from
using them in any commercial pharmaceutical research
conducted in vitro, (3) complied with all applicable United
States and foreign statutes and their respective rules and
regulations in connection with handling, export, import, and
use of the products.
2. AllCells represents and warrants that it has no obligation to
any third party that would interfere with AllCells providing
products to customers. In addition, any products that
AllCells provides to customers are not the proprietary
property of any third party.
3. The Customer agrees that AllCells liability, if any, for
damages (including liability, arising from physical or mental
harm, caused by any product liability, arising out of contract,
negligence, strict liability or tort) shall not exceed the
cost of the product, which the Customer purchased from
AllCells.
4. The Customer agrees that AllCells liability, if any, for
www.allcells.com
For In Vitro Research Use Only
14
Terms & Conditions
damages (including liability, arising from physical or
mental harm, caused by any product liability, arising out of
contract, negligence, strict liability or tort) shall not exceed
the cost of the product, which the Customer purchased
from AllCells.
5. The Customer agrees to indemnify and to hold harmless,
AllCells and all other parties involved, including their
owners, officers, affiliates, associates, and employees
for any and all damages incurred as a result of the
products used. These parties shall not be liable under any
circumstances.
6. By ordering any products from AllCells, the Customer
acknowledges the above terms, conditions, and
disclaimers, included but not limited to the above
disclaimers and any additional warnings and cautions.
The Customer agrees to carefully abide by these terms,
conditions, and disclaimers.
Warranty:
1. AllCells warrants that our products will meet the claimed
product specifications, such as viability, number of viable
cells, and purity. AllCells does not warrant any biological
properties associated with our products.
2. AllCells will replace any products that do not meet the
specifications claimed on our certificate of analysis free
of charge. In no event shall AllCells’ liability exceed the
purchase price paid by the Customer.
3. AllCells shall not be liable for any damages or injuries to
persons or property arising from the use of its products.
AllCells is not liable for a product that has been misused
or has been rendered unusable due to improper storage
or handling.
4. AllCells products are “For In Vitro Research Use Only” and
are neither approved for human nor veterinary use in vivo,
diagnostic, nor clinical applications.
5. This limited warranty has a one-year period starting from
the time of shipment, provided that products have been
stored under AllCells’ recommended storage conditions.
6. AllCells products are not permitted for re-sale without
written authorization from AllCells.
Returns involving fresh cell products must be requested
within 24 hours of product delivery. Returns involving
frozen cell products must be requested within 1 month
of product delivery. Products must be returned in their
original containers with original AllCells label affixed
and unaltered. For product returns to be considered,
customer must certify that product was maintained
under recommended storage conditions while in its
possession, and will be packaged and delivered back to
AllCells according to the same conditions as the original
shipment. Final acceptance of returned product is at the
sole discretion of AllCells. No returns will be accepted for
custom product orders. No return will be accepted without
a written return-authorization number issued by
AllCells.
Disclaimer:
This catalog may contain typographical errors or
inaccuracies and may not be complete or current.
AllCells therefore reserves the right to correct any errors,
inaccuracies or omissions (including after an order has
been submitted) and to change or update information
at any time without prior notice. Please note that such
errors, inaccuracies or omissions may relate to pricing
and availability, and we reserve the right to cancel or
refuse to accept any order placed based on incorrect
pricing or availability information. We apologize for any
inconvenience.
Return Policy:
Catalog products may not be returned for credit without
the express prior written authorization of AllCells, LLC.
A Restocking Fee will be applied to returns in which the
purchaser is at fault. A Restocking Fee of $50 or 15% of
the purchase price will be applied, whichever is greater.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
15
TISSUE COLLECTION FACILITY
Product Description
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
16
TISSUE COLLECTION FACILITY
Background
AllCells, LLC has been operating its own private tissue collection facility under IRB-approved protocols since 2007,
following a 10-year partnership with a local community hospital. Our facility employs a staff of registered nurses, licensed
phlebomtomist, and medical assistants each with multiple years of blood collection experience from healthy donors. A
licensed physician, board certified in the specialties of hematology and oncology, is the medical director and principal
investigator for all our healthy donor related cell collection studies.
We are committed to making the safety and well-being of our donors our first priority. Participating donors receive safe
and attentive care guided by community standards, the FDA, and the State of California Department of Public Health
regulations for blood product donation.
We are dedicated to providing donors with a confidential experience. Donor identity is rigorously protected observing
federally-recognized standards. All staff members are experienced and compliant regarding the protection of human
research subject health information and privacy issues per the Health Insurance Portability and Accountability Act of
1996 (HIPAA) Privacy and Security Rules.
Equipment
A complete blood count (CBC) sample is collected from all donors prior to blood and/or bone marrow donation to verify
donor eligibility. The samples are analyzed on site with results available in about one minute.
All apheresis cell separation procedures are performed by specially trained registered nurses using state-of-the-art,
continuous flow Apheresis System (hardware, software, and disposable kits).
Whole blood collections are completed on site. Samples are collected into receptacles of the customer’s choice, such as
blood transfer packs or vacutainer tubes. Customers may request specific collection receptacles, or provide specific
collection receptacles directly to AllCells, LLC if they prefer.
Bone marrow aspirations are performed by physicians only. A local anesthetic is applied to the donor’s skin and
aspiration site first for donor comfort. Customers have the option of requesting specific collection receptacles.
IRB
All cell collection protocols are overseen and approved by an independent Institutional Review Board (IRB) on an
annual basis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
17
TISSUE COLLECTION FACILITY
Mobilization
AllCells has been a pioneer in the use of G-CSF (Neupogen®) stimulation in normal healthy donors since 1998. The
protocols in use for our Mobilized Peripheral Blood (MPB – pg. 59) products have been approved by an IRB and are
overseen by clinical physicians. In addition, all donors are monitored during the injection, collection procedure and
post-collection to ensure the health of our donors. AllCells is one of only a few providers of Mobilized Peripheral Blood in
the world.
In addition AllCells offers Plerixafor (Mozobil®) mobilization of stem cells from healthy donors. Mozobil, a selective
CXCR4 antagonist, has been particularly beneficial as an adjuvant stem cell mobilization agent on top of the G-CSF
regimen. AllCells is currently the only source of Plerixafor-mobilized cells in the world. For more information on all
available mobilization products and regimens visit our Mobilized Peripheral Blood section on page 59.
Donor Criteria
Non-mobilized Products•
+18 years old for whole blood and 18-55 for Leuko Pak
•
Age 18-65 years old for bone marrow aspiration
•
Donors are screened for HIV (HIV 1 & 2 Ab),
•
Weight > 130 lbs for whole blood
•
Donors cannot be pregnant
•
Weight >140 lbs for Leuko Pak
•
Donors must be in good health
•
Complete Blood Count lab test must meet protocol specs
•
Donors are required to sign consent form prior to collection
Hepatitis B (Surface Antigen HbsAg) & HCV (HCVAb)
Mobilized Products•
All Non-Mobilized criteria
•
Minimum 12 months between donations
•
Age 18-40 years old
•
Twice-in-a-lifetime maximum donation
Capabilities
Having our own tissue collection facility allows us to customize a variety of project variables including protocols,
techniques, equipment, donor characteristics, and delivery.
Customization
Bone Marrow
Apheresis
Custom
Collection
Protocols
•
•
•
•
•
•
Donor
Customization
Other
Customization
ALLCELLS
•
•
•
•
Anticoagulant type ( i.e.
Heparin, ACDA)
Syringe size
Demographics (i.e. age,
ethnicity, gender, etc.)
Past medical history (i.e. health
habits, allergies, medications,
smokers vs. non-smokers, etc.)
HLA typing
ABO typing
CMV positive or negative
Other typing upon request
Same day delivery available for local
customers
•
•
•
•
•
•
•
Whole Blood
Mobilized vs. Non-mobilized
Product volumes ( i.e., cells vs.
autologous plasma)
Product Hematocrit
Demographics (i.e. age,
ethnicity, gender, etc.)
Past medical history (i.e. health
habits, allergies, medications,
smokers vs. non-smokers, etc.)
HLA typing
ABO typing
CMV positive or negative
Other typing upon request
Same day delivery available for local
customers
Toll Free Phone: 1-888-535-3444
www.allcells.com
•
•
•
•
•
•
•
•
Anticoagulant type (i.e. Heparin,
EDTA, ACDA)
Collection receptacles (i.e,
heparinized syringes, vacutainer
tubes, transfer packs, etc.)
Demographics (i.e. age, ethnicity,
gender, etc.)
Past medical history (i.e. health
habits, allergies, medications,
smokers vs. non-smokers, etc.)
HLA typing
ABO typing
CMV positive or negative
Other typing upon request
Same day delivery available for local
customers
For In Vitro Research Use Only
18
TISSUE COLLECTION FACILITY
Standard Collection — Lead Times
When placing your order with our team please keep the following lead times in mind for our standard
collection products:
Collection Type
Lead Time
Mobilized Peripheral Blood (Leuko Pak)
4 weeks
Normal Peripheral Blood (Leuko Pak)
2 weeks
Bone Marrow Aspiration
2 weeks
Whole Blood
1-2 weeks
Matched Sample Collection
In addition to the capabilities illustrated to the left, AllCells has the ability to collect multiple types of tissues from the
same donor on the same day. Various combinations of product types can be mixed and matched including Bone Marrow,
Whole Blood, and Apheresis. Cetain restrictions apply and 3-4 weeks advance notice is required. Please see our ‘Combo
Pak’ product information on page 33 for more information or contact us directly.
Multiple Donor Collection
AllCells Tissue Collection Facility is capable of collecting same tissue types from multiple donors on the same day of
collection (Bone Marrow, Whole Blood, Leuko Pak). Certain restrictions apply and 3-4 weeks advance notice is required.
Contact us for your specific research needs at www.allcells.com/contact-us or fill out our project request form online at:
www.allcells.com/technical-support/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
19
AllCells + Bioservices
Superior primary cells sourcing paired with expert pre-clinical development services
The AllCells Advantage:
Donor Pool
•
+2000 member healthy human donor pool
•
Characterization including HLA, CMV, health history, etc
•
Customization including processing/packaging,
anticoagulant, matched sample collection
Primary Tissue/Cells
•
Leukapheresis packs, whole bone marrow,
peripheral blood
•
Isolated PB-MNC, lymphoid, myeloid, and stem cells
•
Whole blood, isolated cells from diseased donors
including cancer, autoimmune patients
•
Animal primary cells isolated from NHP also
available
Tissue
Culture
Flow Cytometry
ALLCELLS
Toll Free Phone: 1-888-535-3444
Immunoassays
www.allcells.com
For In Vitro Research Use Only
20
BIOSERVICES
Photo credit: BD FACSCanto II®
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
21
BIOSERVICES
Bioservices Table of Contents
Immunoassays22
Hematopoietic Assays23
Flow Cytometry Services
24
Tissue Culture Services
25
Molecular Services 26
Custom HLA-Typing27
Your Trusted Partner in Hematology/Immunology Based Research & Development
Since 1998, AllCells has been the industry leader in providing high quality hematopoietic and immunological cell products to leading
researchers. Our scientists have over 50 years of combined experience developing a wide range of hematopoietic assays. We are
proud to offer the following services as part of our Bioservices division:
Immunoassays
Molecular Services
•
ELISA (single, multiplex)
•
gDNA Extraction
•
ELISpot
•
mRNA cDNA
•
Luminex® (single, multiplex)
Custom HLA-Typing
Hematopoietic Assays
•
Hematopoietic Stem Cell Based Assays (Colony Formation)
•
—CFU-G, CFU-M, CFU-GM, BFU-E
HSC/Progenitor differentiation
Flow Cytometry Services
•
Multivariable Flow Cytometric Analysis, up to 13 colors
•
Immunophenotyping (extracellular and intracellular)
•
DNA Analysis
•
ISHAGE
—CD34 enumeration
—Leukocyte characterization
Tissue Culture Services
•
Luminex® Multianalyte Assays
•
In vitro Proliferation/Inhibition Assays (EC50)
•
CFSE
•
Cell Proliferation Assays (viability, cell counting)
•
MTS/MTT/CTG
•
Toxicology Studies
•
Diseased Cells Analysis
For more information visit: www.allcells.com/bioservices/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
22
BIOSERVICES
Immunoassays
As part of our Bioservices offering, AllCells specializes in Immunoassays. Immunoassays are a robust method of detecting cellular
response and assessing drug efficacy through protein expression/secretion. These assays have industry applications in
autoimmunity, transplantation, vaccine development, therapeutic development, and many others. AllCells provides immunoassay
services including ELISA, ELISpot, Luminex, and Human Donor HLA Typing amongst others.
ELISpot
Enzyme-linked immunospot assay, or ELISpot, is a colorimetric assay which enables quantitative, single cell analysis of protein
expression levels. Due to its high sensitivity and reproducibility, the ELISpot is an industry standard in antigenic response in the form
of cytokine secretion.
T cell proliferation assay
Labeling of T cells with the fluorescent dye 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) allows for quantitative
phenotyping of T cell division in response to antigen or other stimuli by flow cytometric methods. T cell proliferation assays are
essential for peptide epitope mapping of naïve T cells and for characterizing full protein immunogenicity in DC-T cell assays.
Figure. CD4+ (A) and CD8+ (B) T cells were isolated from peripheral human blood and labeled with
5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE). Labeled cells were cultured 7d with or without a stimulatory
antibody cocktail. Fluorescence was measured by flow cytometry.
Luminex (single, multiplex)
The Luminex Bead Array System allows for a high throughput, multi-target analysis of cytokines, proteins, and nucleic acids for
researchers. The system is important for many clinical and research applications.
AllCells + Bioservices can provide custom solutions based on your specific research needs. Contact us to receive a consultation
from one of our scientists: sales@allcells.com.
ALLCELLS
Toll Free Phone: 1-888-535-3444
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For In Vitro Research Use Only
23
BIOSERVICES
Hematopoietic Assays
As a leader in the provision of hematological samples to the research community, AllCells has over 20 years of experience
performing hematopoietic stem cell assays. AllCells offers services in colony formation assays and the expansion and
differentiation of stem and progenitor cells, including hematopoietic and mesenchymal stem cells.
Hematopoietic Stem Cell (HSC) potential is routinely measured through myeloid colony forming units (CFU) in a semi-solid
culture environment. In the presence of cytokines, unipotent progenitor cells are capable of giving rise to colonies with
granulocytes (CFU-G), macrophages (CFU-M), and erythrocytes (BFU-E) while stem/progenitor cells with multipotency give
rise to colonies with multiple lineages (CFU-GM and CFU-GEMM).
In addition to characterization of HSC and MSC potential, AllCells offers services in custom differentiation of our stem and
progenitor cells. AllCells can provide our customers with populations of difficult to isolate cells which have been expanded
and differentiated in culture, including megakaryocytes, dendritic cells and early erythroid cells.
Case study: Samples were treated with different compounds, cultured, and colonies were manually counted. Graph below
displays various colony types after compound treatment.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
24
BIOSERVICES
Flow Cytometry Services
AllCells utilizes our 20+ years of Flow Cytometry experience and state-of-the-art equipment to provide customized research
solutions. We offer Multivariable Flow Cytometric Analysis with up to 13 colors simultaneously using our FACSCalibur®,
FACSCanto II® and FACS Aria II® machines. We also offer custom Multivariable Cell Sorting using our FACS Aria II®. Our
services include Immunophenotyping of intracellular and extracellular molecules, DNA Analysis, and CD34 Enumeration and
Leukocyte Characterization using ISHAGE guidelines.
Our Immunophenotyping services can be applied to cellular characterization of both intracellular and extracellular molecules using
fluorescently-labeled antibodies. These include but are not limited to MHC/HLA Typing, Cancer Cell Analysis (focusing on
Hematological Malignancies), and other custom analyses.
Our DNA Analysis services can provide information on the percentage of cells in each phase of the cell cycle. This output can be
used in drug screening applications, inhibition, proliferation, senescence, etc.
Flow-cytometric enumeration of CD34+ hematopoietic stem and progenitor cells (HSCs) is widely used for evaluation of graft
adequacy of peripheral blood and bone marrow stem cell grafts. The International Society of Hematotherapy and Graft Engineering
(ISHAGE) has created guidelines for a gating strategy that results in replicable standards for stem and progenitor cell enumeration.
AllCells Bioservices adheres to ISHAGE guidelines for characterization of leukocytes and quantification of CD34+ cells.
Fluorescence-activated Cell Sorting (FACS) utilizes the principles of flow cytometry to identify populations of cells of interest.
Cells can be electrically charged and sorted into highly purified populations. At AllCells, we are capable of identification and
sorting of cell populations using up to 11 colors.
AllCells + Bioservices can provide custom solutions based on your specific research needs. Contact us to receive a consultation
from one of our scientists: sales@allcells.com.
Case Study: Extracellular LSK markers were placed on mouse bone marrow cells. LSK markers are an indication of mouse
hematopoietic cells (HSCs) in the mouse bone marrow.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
25
BIOSERVICES
Tissue Culture Services
AllCells Bioservices includes Tissue Culture Services to help study kinetics of cellular response to external stimuli. We offer a wide
range of platforms for analysis of cell proliferation and viability. This can include MTT, MTS, CTG, and Annexin V assays.
Tissue Culture Services include:
•
Luminex® (single, multiplex)
•
In vitro proliferation/inhibition assays (EC50)
•
CFSE (refer to Flow Cytometry Services pg. 24)
•
Cell proliferation assays (viability, cell counting)
MTS/MTT/CTG – To study the kinetics of cell viability in drug compound screening, we are able to implement specific assays.
Alternatively, these assays may be used for inhibition studies, illustrating the kinetics of your cells: EC50/IC50 analysis
Toxicology Studies – Our bioservices can help with QC validation of toxic effects on cells by certain compounds.
Diseased Cells – AllCells serves as a reliable source for a variety of hematological, oncologic, and autoimmune diseased cells that
can be used with multiple bioservices including compound screening, biomarker discovery, and characterization of specific
diseased cells. We source our own cells, have control over our supply, and can customize based on research demands.
Compound treatment is one example of our Tissue Culture Services:
Proliferation - grow cells, add reagent,
incubate, measure live cells labeled
Apoptosis (Annexin V/PI)
Compound Treatment - aliquot cells,
treat with drug compounds
Pathway Activation
CFU-C Assay
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
26
BIOSERVICES
Molecular Services
As part of AllCells Bioservices we offer molecular services. Our molecular services focus on genomic DNA extraction for
HLA genotyping, and Fc-gamma Receptor IIIa (CD16a typing). Molecular services are useful for minimizing variation in your
sample population to increase reproducibility in downstream applications.
gDNA Isolation
Our Bioservices can package genomic DNA extraction with mutational analysis, genomic profiling, and other sequencebased analyses.
The advancement in genetic engineering has transcended genomic DNA from the basic code of life to the pivotal tool
for next generation medicine. From gene therapy to cellular reprogramming, the DNA molecule continues to manifest
its dynamic potentials.
Because the research needs for genomic DNA may or may not be cell-type dependent, AllCells offers a customized service to
extract and purify genomic DNA from any of our available primary cell products, human as well as other available mammalians,
and from any other mammalian tissues such as human tumors provided by the research client.
AllCells also features cell-type and donor matched extractions of both genomic DNA and comprehensive RNA. Pricing may vary.
AllCells + Bioservices can provide custom solutions based on your specific research needs. Contact us to receive a
consultation from one of our scientists: sales@allcells.com.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
27
BIOSERVICES
Custom HLA-Typing
Human Leukocyte Antigen, or HLA, plays a strategic role in the recognition and defense mechanisms of the human body.
The segregation and pooling of the human genome have created a wide spectrum of diverse HLA. Many research efforts are
underway to employ different HLA haplotypes for aid in identifying treatment for infectious diseases, autoimmune diseases
and cancer.
Applications for HLA-typed cells:
1.
Cellular Immunotherapy (CAR T cells or DC immunotherapy)
2.
Transplantation Research and MLR assays
3.
Vaccine Development
4.
Drug screening in HLA-specific cohorts
5.
Generating humanized mice
6.
Infectious disease research
7.
Metabolic and Autoimmune Disease research
Advantages of AllCells HLA-typed cells:
•
many lots of frozen cells in inventory already typed
•
can specify collection of fresh blood, bone marrow, PB-MNC in HLA-characterized donors
•
over 50 donors typed by Next Generation Sequencing (high resolution) for ALL HLA Class I and Class II loci (A, B, C
DPB1, DRB1, DQA1, DQB1)
•
over 200 donors typed by SSO (low resolution) for HLA-A
Contact us for any HLA questions or share your project with us at: sales@allcells.com or www.allcells.com/contact-us
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
28
HUMAN PRIMARY CELLS
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
29
HUMAN PRIMARY CELLS
Human Primary Cells & Tissue Table of Contents
Non-Diseased Custom Products
30
Normal Peripheral Blood
32-57
Mobilized Peripheral Blood
58-64
Bone Marrow65-72
Cord Blood
73-85
Non-Hematopoietic Stem Cells
86-87
Diseased Cells88-120
Human Primary Cells & Tissue
Human cells & tissue are the most clinically-relevant model for research. AllCells provides a wide range of primary human
cells. These human primary cells include hematopoietic and immunological cells, mesenchymal cells, amniotic and placental
cells, and dental pulp cells.
These human cells are made available from numerous sources. Our normal/healthy human cells are sourced from healthy
peripheral blood, G-CSF mobilized peripheral blood, bone marrow, cord blood, amniotic & placental fluid, dental pulp and
adipose tissue. Our diseased human cells are sourced from either peripheral blood or bone marrow.
AllCells human cells product line is available in fresh or frozen format. Please contact us for any special requests or custom
isolations at www.allcells.com/technical-support/ or sales@allcells.com.
For more information visit: www.allcells.com/human/
ALLCELLS
Toll Free Phone: 1-888-535-3444
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For In Vitro Research Use Only
30
NON-DISEASED CUSTOM PRODUCTS
AllCells offers a variety of customized options for non-diseased products(for disease products see page 90): donor
recruitment, collection, cell isolation, processing and services. Our Custom Product/Project Request Form appears on the
following pages and our website. For more information see our Tissue Collection Facility Capabilities on page 15.
Examples of custom primary cell isolations but not limited to:
•
Custom cell isolations, small and large projects
•
Lysate and cell pellet
•
Custom cell numbers
•
Custom processing/packaging:
-Frozen or snap frozen for RNA or cDNA
-Lysis with custom buffer/reagents
-Custom collection into tubes/blood bags
-PAXgene/Cyto Chex tubes
-Anticoagulant of choice
-Custom handling
-Custom gradient density PBMC isolation
•
Multiple cell types from a single donor
•
Donor-matched cells
-Matching plasma, serum, platelets, and RBC from the same donor
-Matching whole blood and bone marrow
•
-ABO blood typed matching donors
Hematopoietic stem cells from mobilized peripheral blood
For more information visit: www.allcells.com/custom/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
31
HEALTHY PRIMARY CELLS & TISSUE
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
32
NORMAL PERIPHERAL BLOOD
Normal Peripheral Blood Table of Contents
Apheresis Collection (Leuko Pak)
33
Whole Peripheral Blood34-35
Plasma36
Mononuclear Cells (MNC)37
Stem/Progenitor Cells (CD34+)38
Lymphoid Cells
B Cells39-40
(CD19+, CD19+/IgD+)
T Cells41-47
(CD3+ Pan, CD4+ Helper, CD4+/CD25+,
CD4+/CD45RA+/CD25- Naive Helper,
CD4+/CD45RO+ Memory, CD8+ Cytotoxic,
CD8+/CD45RA+ Naive Cytotoxic)
Dendritic Cells (BDCA4+ Lymphoid/Plasmacytoid)
48
Natural Killer Cells (CD56+)49
Myeloid Cells
Basophils
50
Eosinophils51
Neutrophils52
Red Blood Cells/Mature Erythrocytes 53
Platelets 54
Monocytes (CD14+)55
Dendritic Cells (Monocyte-Derived)
56
Macrophages57
Donor Criteria
•
Age: 18-55 years old
-Blood and bleeding disorders, including sickle cell disease
•
Weight: >140 lbs for Leuko Pak
-Neurologic disorders
•
Weight: > 130 for whole blood
-Autoimmune disorders
•
CBC lab tests must meet protocol specifications.
-Cancer
•
Donors cannot be pregnant.
-Diabetes
•
Donors are screened for HIV, Hepatitis B and Hepatitis C
-Positive: HIV, HbsAg, HCAb
•
before donation.
•
Donors must have adequate peripheral veins for apheresis.
Donors must be in general good health. Donors with any of the
•
Donors are required to sign a procedure-specific consent form
following will be excluded from donating:
prior to the collection procedure.
-History of heart, lung, liver, or kidney disease
-History of asthma
For more information visit: www.allcells.com/normal-peripheral-blood/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
33
Normal Peripheral Blood
Normal Peripheral Blood—Leuko Pak (Apheresis Collection)
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB007-2
1/4 Bag
$1659
PB007-1
1/2 Bag
$1999
PB007
1 Bag
$2949
PB007-3A
1st 1/2 Bag
$3037
PB007-3B
2nd 1/2 Bag
$2507
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Leuko Pak
Product Description
Application
AllCells Leuko Paks are highly-enriched leukapheresis-derived
products obtained from our own IRB-approved collection facility. These products contain higher concentrations of mononuclear cells, B cells, T cells, stem/progenitor cells, dendritic
cells, and other cell types as compared to standard venipuncture collection methods or buffy coat products.
Full/Half/Quarter Pak - Normal peripheral blood mononuclear
cells are collected from healthy donors in various collection
sizes. For the Full Leuko Pak, approximately 3 blood volumes
are processed using the COBE SpectraTM apheresis machine
and between 50 and 150 mL of autologous plasma is collected at the same time as the mononuclear cells.
Dual Half Pak - PB007-3A, -3B - A healthy donor undergoes
an apheresis procedure using the COBE SpectraTM machine
on two separate occasions. The same donor is reserved for a
second apheresis procedure 6-12 weeks after the first. Each
time, 1.5 blood volumes are processed and approximately 100
mL of normal peripheral blood mononuclear cells and 50 mL of
autologous plasma are collected. Second Half Pak from same
donor is optional.
NPB Leuko Paks can be used to:
• Isolate large-scale dendritic cells.
• Profile and screen normal peripheral blood monocytes
and lymphocytes.
• Study intercellular interactions by using the same-donor
leukocytes directly or in culture.
Lead Time
A minimum of two weeks is needed.
Related Products
G-CSF Mobilized Peripheral Blood Leuko Pak
Published Papers
Xiao F., et al. (2013) The Genome of Polymorphonuclear Neutrophils
Maintains Normal Coding Sequences. PLoS ONE 8(11).
http://www.allcells.com/publications/0203allcells.pdf
For more information visit: www.allcells.com/leuko-pak/
Combo Pak - AllCells also provides an option to obtain Bone
Marrow (50 or 100 mL), Whole Peripheral Blood (up to 120
mL), and a Full Leuko Pak from the same donor at the same
time. Any and all combinations of these products are available.
Inquire for more information.
Leuko Pak
¼ Bag: ½ Bag: 1 Bag: Cell Number Ranges
Average: 0.37x1010
Average: 0.75x10 10
Average: 1.5x10 10
ALLCELLS
Range: 0.1 to 0.7x1010
Range: 0.6 to 1x1010
Range: 1 to 3.5x1010
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
34
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
WB001-ACD
60 ml
$346
WB001-NC
60 ml
$346
WB001-NH
60 ml
$346
WB001-VACD
60 ml
$353
WB001-VK2
60 ml
$353
WB001-VNH
60 ml
$353
WB002-ACD
120 ml
$618
WB002-NC
120 ml
$618
WB002-NH
120 ml
$618
WB002-VACD
120 ml
$626
WB002-VK2
120 ml
$626
WB002-VNH
120 ml
$626
WB003-ACD
180 ml
$866
WB003-NC
180 ml
$866
WB003-NH
180 ml
$866
WB003-VACD
180 ml
$871
WB003-VK2
180 ml
$871
WB003-VNH
180 ml
$871
WB004-ACD
240 ml
$1117
HLA-Typed Products are available upon request.
Product Description
Application
Whole blood (WB) is anti-coagulated using heparin(catalog#
notation "NH"), ACD:A(catalog# notation "ACD"), EDTA
(catalog# notation "K2") or sodium citrate(catalog# notation
"NC") per customer specification. Our standard anticoagulant
is heparin (5 U/ml of WB). The volume of whole blood removed
per donation may vary, but will not exceed 420 mL at any
one time. Whole blood collection quantities are in volume
increments of 60 mL up to a maximum of 420 mL however
custom collections in increments of 10 ml are available upon
request. Please contact us if smaller volumes are needed. WB
can be collected in vacutainer tubes("V" in catalog number),
syringes, or transfer packs.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Whole Blood can be used to study:
• Diagnostic or early detection tools for different
diseases, blood-related or unrelated.
• Serum and plasma proteins and their pathway
functions, such as metabolism and immunity.
• Therapeutic antibodies or other drug screenings.
Lead Time
A minimum of 1-2 week is needed.
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Whole Blood
35
Normal Peripheral Blood
Normal Peripheral Blood—Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
WB004-NC
240 ml
$1117
WB004-NH
240 ml
$1117
WB004-VACD
240 ml
$1125
WB004-VK2
240 ml
$1125
WB004-VNH
240 ml
$1125
WB005-ACD
300 ml
$1355
WB005-NC
300 ml
$1355
WB005-NH
300 ml
$1355
WB005-VACD
300 ml
$1373
WB005-VK2
300 ml
$1373
WB005-VNH
300 ml
$1373
WB006-ACD
360 ml
$1469
WB006-NC
360 ml
$1469
WB006-NH
360 ml
$1469
WB007-ACD
420 ml
$1608
WB007-NC
420 ml
$1608
WB007-NH
420 ml
$1608
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-WB
Product Description
Lead Time
Whole blood (WB) is anti-coagulated using heparin, ACD:A or
EDTA per customer specification. Our standard anticoagulant
is heparin (5 U/ml of WB). The volume of whole blood removed
per donation may vary, but will not exceed 420 mL at any
one time. Whole blood collection quantities are in volume
increments of 60 mL up to a maximum of 420 mL however
custom collections in increments of 10 ml are available upon
request. Please contact us if smaller volumes are needed. WB
can be collected in vacutainer tubes("V" in catalog number),
syringes, or transfer packs.
A minimum of 1-2 week is needed.
Published Papers
Noto, Paul B., et al. (2012) Regulation of Sphingomyelin Phosphodiesterase, acid-like 3A gene (SMPDL3A) by Liver X Receptors.
Molecular Pharmacology, 112.078865.
http://www.allcells.com/publications/0162allcells.pdf
Zhao, Zhiwei, et al. (2008) Induction of CYP4F3 by Benzene Metabolites in Human White Blood Cells in Vivo Promeylocytic Leukemic Cell
Lines and ex Vivo in Human Blood Neutrophils. Drug Metabolism and
Disposition, 37 (2), 282-291.
http://www.allcells.com/publications/0083allcells.pdf
Vose, Sara C., et al. (2007) Lysophosphatidylcholine Hydrolases of
Human Erythrocytes, Lymphocytes and Brain: Sensitive Targets of
Conserved Specificity for Organophosphorus Delayed Neurotoxicants.
Toxicol Appl Pharmacol., 224(1), 98–104.
http://www.allcells.com/publications/0089allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
36
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB017
10 ml
$111
PB018
20 ml
$156
PB019
30 ml
$193
PB020
40 ml
$230
PB021
50 ml
$256
PB022
100 ml
$323
PB023
150 ml
$378
PB017F
10 ml
$86
PB018F
20 ml
$131
PB019F
30 ml
$168
PB020F
40 ml
$206
PB021F
50 ml
$231
PB022F
100 ml
$298
PB023F
150 ml
$361
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Plasma
Lead Time
Product Description
NPB Plasma is obtained from healthy donors through the
COBE Spectra™ apheresis machine. Between 50 and 150
mL of autologous plasma is collected at the same time as
the mononuclear cells.
Application
NPB-Plasma can be used to:
• Analyze blood plasma components.
• Study plasma transfusion efficiency for coagulopathy
and liver diseases.
• Function as source of serum if platelets, fibrinogen, and
other coagulating factors are removed.
• Serve as a control in studies on Factor VIII and
coagulopathies, such as hemophilia.
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of 1-2 weeks is needed.
Related Products
ALL Plasma
AML Plasma
CB Plasma
Published Papers
Francisco, Joseph A. et al. (2003). cAC10-vcMMAE, an anti-CD30–
monomethyl auristatin E conjugate with potent and selective
antitumor activity. Blood, 102(4), 1458-1465.
http://www.allcells.com/publications/0050allcells.pdf
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Plasma
37
Normal Peripheral Blood
Normal Peripheral Blood—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB001
150 million/vial
$469
PB002
350 million/vial
$611
PB003F
15 million/vial
$129
PB006F
25 million/vial
$189
PB004F
50 million/vial
$279
PB005F
100 million/vial
$439
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-MNC
Table: NPB Cell Subset Products
Product Description
Mononuclear cells are collected from fresh blood during
apheresis into Leuko Paks which enriches lymphocytes
and monocytes while depleting mature granulocytes. The
mononuclear fraction is then further processed to eliminate red
blood cells and platelets.
NPB Cell Subsets
Average Percentage in MNC
CD34+
0.2%
CD3
60%
CD4
30%
+
+
CD8
23%
+
CD14
15%
CD19
9%
CD56
7%
Basophils
0.3%
Neutrophils and Eosinophils
0.2%
RBC
<0.01%
Platelets
<0.01%
+
Application
+
NPB-MNC can be used to:
• Isolate and study MNC subpopulations.
• Act as controls for therapeutic research, involving
lymphocytes and monocytes.
• Study the relationship among MNC subpopulation
(for example, the relationship among T Cells, B Cells, and
monocytes)
• Research anti-GVHD drugs.
Lead Time
+
The table above is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and not
the total white blood cell population. We cannot guarantee the recovery of purifica-
tion products listed above. For a specific number of a certain cell type, please consider
our normal peripheral blood purified products.
Related Products
MPB-MNC
A minimum of two weeks is needed for fresh cells.
BM-MNC
CB-MNC
Published Papers
Arastu-Kapur, Shirin, et al. (2011) Nonproteasomal Targets of the Proteasome Inhibitors Bortezomib and Carfilzomib: a Link to Clinical Adverse
Events. Clinical Cancer Research, 17, 2734.
http://www.allcells.com/publications/0160allcells.pdf
Xu, Yuanxin, et al. (2008) Validation of a HLA-A2 tetramer flow cytometric
method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma
patients. Journal of Translational Medicine, 6(61).
http://www.allcells.com/publications/0033allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
38
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB032-1
0.5 million/vial
$2151
PB032-2
1 million/vial
$3751
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD34+ Stem/Progenitor Cells
Product Description
Purity
NPB-CD34+ stem/progenitor cells (NPB-CD34+) are
positively isolated from mononuclear cells using an
immunomagnetic CD34 MicroBead labeling system.
Application
Lead Time
NPB-CD34+ cells can be used to:
• Study expansion, lineage generation, and transplantation
potentials.
• Serve as an alternative, less invasive blood source, to
bone marrow.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Note: NPB-CD34+ cells require more source volume due
to low percentages in peripheral blood.
A minimum of two weeks is needed for fresh cells.
Related Products
BM-CD34+ Cells CB-CD34+ Cells MPB-CD34+ Cells
Published Papers
Mack, Amanda A., et al. (2011) Generation of Induced Pluripotent
Stem Cells from CD34+ Cells across Blood Drawn from Multiple Donors with Non-Integrating Episomal Vectors. PLoS ONE, 6(11).
http://www.allcells.com/publications/0138allcells.pdf
Iancu-Rubin, Camilia, et al. (2011) Down-regulation of stathmin
expression is required for megakaryocyte maturation and platelet
production. Blood, 117(17).
http://www.allcells.com/publications/0149allcells.pdf
Dongjiu, Ye, et al. (2009) Analysis of Aurora kinase A expression in
CD34+ blast cells isolated from patients with myelodysplastic syndromes and acute myeloid leukemia. J Hematopathol, 2, 2-8.
http://www.allcells.com/publications/0034.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Stem/Progenitor Cells—CD34 + Cells
39
Normal Peripheral Blood—B Cells—CD19 + B Cells
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB010
10 million/vial
$1027
Frozen
PB010F
10 million/vial
$854
Fresh
PB010-P
10 million/vial
$830
Frozen
PB010-P-F
10 million/vial
$755
Positively Selected Cell Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD19+ B Cells
Purity
Product Description
Untouched NPB-CD19+ B Cells are negatively isolated from
mononuclear cells using an indirect immunomagnetic labeling
system.
Positively Selected NPB-CD19+ B Cells are directly isolated
from mononuclear cells using immunomagnetic CD19
MicroBeads.
Untouched NPB-CD19+ B Cells: The purity is guaranteed to be
at least 85% as assessed by flow cytometric analysis.
Positively Selected NPB-CD19+ B Cells: The purity is
guaranteed to be at least 90% as assessed by flow cytometric
analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-B Cells can be used to research:
• Cell-mediated immunity pathway
• B cell activation and cloning mechanisms
• Genetic rearrangements of immunoglobulins
• Antigen presentation
• Recognition mechanisms
• Cloning of memory and plasma cells
• Antibody and immunoglobulin secretion
• Toll-like receptors
Related Products
CB-B Cells MPB-B Cells
Published Papers
Akers, Stephen M., et al. (2010) VE-cadherin and PECAM-1 enhance ALL
migration across brain microvascular endothelial cell monolayers. ISEH-Society for Hematology and Stem Cells, doi: 10.1016/j.exphem.2010.05.001.
Mahadevan, Daruku, et al. (2009) Gene Expression and Serum Cytokine
Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3, and CXCL9/
CXCR3 as Regulators of Cell Proliferation, Survival, and Migration. SAGEHindawi Access to Research Human Genomics and Proteomics, 2009.
http://www.allcells.com/publications/0064allcells.pdf
Baysa, Bora E., et al. (2007) A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and
Childhood T-Cell Acute Leukemia Bora E. PLoS ONE, 5(e46).
http://www.allcells.com/publications/0012allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
40
Normal Peripheral Blood—B Cells—CD19 +/IgD + Naïve B Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh/Untouched
PB010-3
1 million/vial
$1231
Frozen/Untouched
PB010-3F
1 million/vial
$1221
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD19+/IgD+ B Cells
Purity
Product Description
NPB-CD19+/IgD+ B cells are negatively isolated
from MNC using immunomagnetic isolation system to
deplete non-naive B cells.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-Memory B Cells can be used to study:
• Cell-mediated immunity pathway
• B cell activation and cloning mechanisms
• Signal transductions
• Antigen uptake and presentation
• Antibody and immunoglobulin secretion
• Cognate interaction of naive B cells with T helper
cells or dendritic cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Cell Products
41
Normal Peripheral Blood—T Cells—CD3 + Pan T Cells
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-1
20 million/vial
$845
Frozen
PB009-1F
20 million/vial
$714
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB- Pan T Cells (CD3+)
Product Description
NPB-Pan T Cells are negatively isolated from mononuclear
cells using an indirect immunomagnetic Pan-T labeling system.
Application
NPB-Pan T Cells can be used to research the following:
• T cell activation
• Maintenance of the naïve T cells (CD45RA+)
• Transformation to memory (CD45RO+) and or regulatory (CD25+) T cells, and their respective functions
• Intercellular T-cell regulatory and activation pathways
• Antigen presentation and other receptor pathways
• Recognition mechanisms
Related Products
CB-Pan T Cells
MPB-Pan T Cells
Published Papers
Yang, Jianchang, et al. (2008) SALL4 is a key regulator of survival and apoptosis in human leukemic cells. Blood. doi: 10.1182/
blood-2007-11-126326.
http://www.allcells.com/publications/0106allcells.pdf
Mahadevan, Daruku, et al. (2005) Transcript profiling in peripheral
T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signatures. Mol Cancer Ther,
4(12). http://www.allcells.com/publications/0112allcells.pdf
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
Purity
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells. Frozen
products are subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
42
Normal Peripheral Blood—T Cells—CD4 + Helper T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-2
15 million/vial
$832
Frozen
PB009-2F
15 million/vial
$716
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+ Helper T Cells
Product Description
Purity
NPB CD4+ Helper T cells are negatively isolated from
mononuclear cells using an indirect immunomagnetic CD4 + T
cell labeling system.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-CD4+ Helper T Cells can be used to study:
• Effect on B cell antibody class switching
• HIV infection mechanisms and therapeutic research
• Antigenic presentation, intracellular activation
mechanisms, and learned immunity
• Mechanism and therapeutics of hypersensitivity and
autoimmune disease
Related Products
CB-CD4+ T Cells
MPB- CD4+ T Cells
Published Papers
Mahadevan, Daruku, et al. (2005) Transcript profiling in peripheral
T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signatures. Mol Cancer Ther,
4(12). http://www.allcells.com/publications/0112allcells.pdf
Lu, Xiaobin, et al. (2004) Antisense-Mediated Inhibition of Human
Immunodeficiency Virus (HIV) Replication by Use of an HIV Type
1-Based Vector Results in Severely Attenuated Mutants Incapable of
Developing Resistance. Journal of Virology, 78(13), 7079-7088.
http://www.allcells.com/publications/0043allcells.pdf
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Cell Products
43
Normal Peripheral Blood—T Cells—CD4 +/CD25 + T Cells
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-4
2 million/vial
$977
Frozen
PB009-4F
2 million/vial
$873
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+/CD25+ T Cells
Purity
Product Description
First, CD4+ T Cells are isolated using an indirect
immunomagnetic depletion. Next, CD25+ cells are positively
isolated using CD25 MicroBeads, leaving a highly purified
CD4+/CD25+ Regulatory T Cell population.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
NPB-CD4+/CD25+ Regulatory T Cells can be used to study:
• Homeostasis in the immune system.
• Hypersensitivity and autoimmunity.
• Methods to improve the body’s defense against infection
and tumors.
• Activated T cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
44
Normal Peripheral Blood—T Cells—CD4 +/CD45RA+/CD25 - Naïve Helper T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-5
5 million/vial
$1026
Frozen
PB009-5F
5 million/vial
$917
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+/CD45RA/CD25- Naïve Helper T Cells
Product Description
Purity
NPB-CD4+/CD45RA+/CD25- Naive Helper T Cells are
negatively isolated from mononuclear cells using an indirect
immunomagnetic CD4+/CD45RA+/CD25- labelling system.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
NPB-CD4+/CD45RA+ Naïve T Cells can be used to study:
• Naïve T cell activation
• Memory T Cell training and activation for long-term
immunity
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
CB-CD4+/CD45RA+ T Cells
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Cell Products
45
Normal Peripheral Blood—T Cells—CD4 +/CD45RO + Memory T Cells
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-7
5 million/vial
$1026
Frozen
PB009-7F
5 million/vial
$979
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD4+/CD45RO+ Memory T Cells
Purity
Product Description
NPB-CD4+ Memory T Cells are negatively isolated from
mononuclear cells using an indirect immunomagnetic CD4 +/
CD45RO+ labelling system.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-CD4 /CD45RO Memory T Cells can be used to study:
• The capacity for self-renewal (as a result of high levels of
phosphorylation and the transcription factor STAT5)
• Memory T cell development and maintenance specificity
for different antigens
• Memory T Cell activation mechanisms and pathways
+
ALLCELLS
+
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
46
Normal Peripheral Blood—T Cells—CD8 + Cytotoxic T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-3
10 million/vial
$818
Frozen
PB009-3F
10 million/vial
$717
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD8+ Cytotoxic T Cells
Purity
Product Description
NPB-CD8+ Cytotoxic T cells are negatively isolated from mononuclear cells using an indirect immunomagnetic CD8 + T cell
labeling system.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-CD8 T Cells can be used to study:
• VDJ recombination mechanisms of the TcR (T cell
receptor), self/non-self recognition, and autoimmunity
• Cytotoxic cell lysis and platelet activation to infected
tissues or organs
• Defense mechanisms against tumor and viral infections
+
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
CB-CD8+ T Cells
MPB- CD8+ T Cells
Published Papers
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Cell Products
47
Normal Peripheral Blood—T Cells—CD8 +/CD45RA+ Naive Cytotoxic T Cells
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB009-6
5 million/vial
$1026
Frozen
PB009-6F
5 million/vial $898
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD8+/CD45RA+ Naive Cytotoxic T Cells
Product Description
Purity
NPB-CD8+/CD45RA+ Naive Cytotoxic T cells are negatively
isolated from mononuclear cells using an indirect
immunomagnetic CD8+/CD45RA+ T cell labeling system.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-CD8+/CD45RA+ Naïve Cytotoxic T Cells can be used
to study:
• Homeostasis of Naive Cytotoxic T Lymphocytes in its
surrounding repertoire
• Activation of Naïve Cytotoxic T Lymphocytes
• Methods to redirect the Cytotoxic T Lymphocytes learning
pathway for autoimmune therapy
• In vitro engineering to fight specific targets, such as
viruses or tumors
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
48
Positively Selected Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
PB013F
0.5 million/vial
Inquire
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-BDCA4+ Lymphoid/Plasmacytoid Dendritic Cells
Purity
Product Description
NPB-BDCA4 + Lymphoid/Plasmacytoid Dendritic cells have
been positively isolated from mononuclear cells using
immunomagnetic BDCA-4 MicroBead labeling system.
BDCA-4 is marker for CD11c-, CD123bright (lymphoid) dendritic
cells. NPB-BDCA4+ cells possess plasmacytoid morphology
and express the CD45RA cell marker. BDCA-4 is not
detectable on the cell surface of other blood cells.
NPB-BDCA4 + cells can be used to study:
• Antigen presentation
• Activation of toll-like receptors and other pattern
recognition receptors of viral immunity pathways
• Interferon alpha secretion
• Potential uses in anti-tumor research
ALLCELLS
Lead Time
A minimum of two weeks is needed for fresh cells.
Related Products
MPB-BDCA4 + T Cells
Application
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
Published Papers
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Dendritic Cells—BDCA4 + Lymphoid/
Plasmacytoid Dendritic Cells
49
Normal Peripheral Blood—CD56 + Natural Killer Cells
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB012
5 million/vial
$990
Frozen
PB012F
5 million/vial
$855
Fresh
PB012-P
5 million/vial
$806
Frozen
PB012-P-F
5 million/vial
$696
Positively Selected Cell Products
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD56+ Natural Killer Cells
Lead Time
Product Description
Untouched NPB-CD56+ Natural Killer (NK) cells are negatively
selected from mononuclear cells using an indirect
immunomagnetic NK cell labeling system to deplete the
non-CD56+ NK cells.
Positively Selected NPB-CD56 NK cells are directly isolated
from mononuclear cells using direct immunomagnetic CD56
MicroBeads.
+
Application
A minimum of two weeks is needed for fresh cells. For all other
products, product delivery is subject to stock availability.
Related Products
CB Natural Killer Cells
MPB Natural Killer Cells
Published Papers
Begovich, Ann B., et al. (2004) A Missense Single-Nucleotide
Polymorphism in a Gene Encoding a Protein Tyrosine Phosphatase
(PTPN22) Is Associated with Rheumatoid Arthritis. Am. J. Hum.
Genet., 75, 330-337.
http://www.allcells.com/publications/0022allcells.pdf
NPB-NK Cells can be used to study:
• Innate immune system against tumors and viral infections
• Inhibitory receptor recognition and activation
• Cytolytic mechanisms
• Antibody dependent cellular cytotoxicity assay (ADCC)
• FcR binding affinity
• Therapeutic antibody development
Purity
Untouched NPB-CD56+ Cells: The purity is guaranteed to be
at least 85% as assessed by flow cytometric analysis.
Positively Selected NPB-CD56+ Cells: The purity is guaranteed
to be at least 90% as assessed by flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
50
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB014
0.3 million/vial
$1036
Frozen
PB014F
0.3 million/vial
$898
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Basophils
Product Description
Purity
NPB-Basophils are isolated from peripheral blood
mononuclear cells using an indirect immunomagnetic basophil
labeling system.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of two weeks is needed for fresh cells.
NPB-Basophils can be to study the following:
• Inflammatory responses, involving mast cell differentiation,
IgE expression, and histamine release. The study of these
responses and pathways can lead to remedies for allergy
and asthma.
• Anti-allergy remedies, such as transient transfection
of basophils (inhibiting IgE expression) and other
extracellular methods of basophil suppression.
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
BM-Basophils
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Basophils
51
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Eosinophils
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB015
1 million/vial
$1025
Frozen
PB015F
1 million/vial
$897
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Eosinophils
Product Description
Purity
RBC depleted leukocytes are collected from peripheral blood
by sedimentation of red blood cells using HetaSep. Then, the
granulocytes are isolated by density gradient separation and
further processed to deplete any remaining RBC. Finally, the
eosinophils are isolated using an indirect immunomagnetic
labeling system.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of one week is needed for fresh cells.
Application
NPB-Eosinophils can be used to study:
• Post-pubertal mammary gland development
• Estrous cycles
• Allograft rejection
• Neoplasia
• Antigenic presentation to T cells
• Treatment and prevention for atopic dermatitis
• Degranulation and release mechanisms for peroxidases,
nucleases, plasminogens, and major basic proteins
• Cytokine and interleukin release by activation
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
BM-Eosinophils
CB-Eosinophils
www.allcells.com
For In Vitro Research Use Only
52
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB016
40 million/vial
$886
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Neutrophilis
Purity
Product Description
RBC depleted leukocytes are collected from peripheral blood
by sedimentation of red blood cells using HetaSep. Then, the
granulocytes are isolated by density gradient separation and
further processed to deplete any remaining RBC. Finally, the
neutrophils are isolated using an indirect immunomagnetic
labeling system.
Application
NPB-Neutrophils can be used to study:
• Acute inflammation and tissue injury response
• Degranulation mechanisms
• Function of neutrophils to form NET (neutrophil extracellular traps) that act as a bacterial killing pathway
independent of phagocytosis
• Neutropenia (naturally occurring or chemotherapy
induced).
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of one week is needed for fresh cells.
Related Products
BM-Neutrophils
CB-Neutrophils
Published Papers
Zhao, Zhiwei, et al. (2008) Induction of CYP4F3 by Benzene Metabolites in Human White Blood Cells in Vivo Promeylocytic Leukemic Cell
Lines and ex Vivo in Human Blood Neutrophils. Drug Metabolism and
Disposition, 37 (2), 282-291.
http://www.allcells.com/publications/0083allcells.pdf
Brogdon, Jennifer, et al. (2007). Histone deacetylase activities are
required for innate immune cell control of Th1 but not Th2 effector
cell function. Blood, 109(3), 1123-1130.
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Neutrophils
53
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Mature Erythrocytes(RBC)
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB029
80 billion/vial
$451
PB030
160 billion/vial
$561
PB031
240 billion/vial
$813
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Mature Erythrocytes / Red Blood Cells
Purity
Product Description
NPB Mature Erythrocytes, also referred to as red blood cells
(RBC), are obtained from normal peripheral blood using
filtration method.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of one week is needed.
NPB-RBC can be used to study anemia caused by
(but not limited to):
• Sickle cell disease
• Thalassemia
• Spherocytosis
• Pernicious anemia
• Aplastic anemia
• Pure red blood cell aplasia
• Hemolysis
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
54
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB025
5 billion/vial
$193
PB026
10 billion/vial
$323
PB027
20 billion/vial
$451
PB028
30 billion/vial
$580
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Platelets
Product Description
Lead Time
NPB-Platelets are obtained from healthy donors with the
collection of maximum three-blood volumes on a COBE
Spectra™ apheresis machine. The platelets are shipped in
platelet-rich plasma.
Application
NPB platelets can be used to study the following:
• Aggregation and coagulation mechanisms
and pathways
• Atherosclerosis
• Coronary artery disease
• Myocardial infarction
• Cerebrovascular diseases
• Stroke
• Peripheral artery occlusive disease
• Cancer, including prevention and treatment of transfusion
related complications
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells.
Published Papers
Kubota, Satoshi, et al. (2004). Abundant retention and release of
connective tissue growth factor (CTGF/CCN2) by platelets. Journal
of Biochemistry, 136(3), 279-282.
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Normal Peripheral Blood—Myeloid Cells—Platelets
55
Normal Peripheral Blood—Myeloid Monocytes—CD14 + Monocytes
Normal Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PB011
10 million/vial
$990
Frozen
PB011F
10 million/vial
$854
PB011-P-1
10 million/vial
$830
PB011-P-2
20 million/vial
$1067
PB011-P-3
40 million/vial
$1409
PB011-P-F-1
10 million/vial
$716
PB011-P-F-2
20 million/vial
$934
PB011-P-F-3
40 million/vial
$1361
Positively Selected Cell Products
Fresh
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-CD14+ Monocytes
Product Description
Purity
Untouched NPB-CD14+ Monocytes are indirectly isolated
from mononuclear cells using an immunomagnetic
labeling system.
Positively-Selected NPB-CD14+ Monocytes are directly
isolated from mononuclear cells using an immunomagnetic
CD14 MicroBead labeling system.
Application
NPB-CD14 Monocytes can be used to study:
• Post-pubertal mammary gland development
• Estrous cycle
• Allograft rejection
• Neoplasia
• Antigenic presentation to T cells
• Treatment and prevention for atopic dermatitis
• Toll-like receptors and other pattern recognition
receptors
+
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 85% for untouched
monocytes, and at least 90% for positively selected
monocytes.
Lead Time
A minimum of two weeks is needed for fresh cells.
Related Products
CB- Monocytes
MPB-Monocytes
Published Papers
Wang, Jinghong, et al. (2006) Kynurenic Acid as a Ligand for Orphan
G Protein-coupled Receptor GPR35. The Journal of Biological Chemistry, doi: 10.1074/jbc.M6035003200.
http://www.allcells.com/publications/0085allcells.pdf
Braselmann, Sylvia, et al. (2006). R406, an Orally Available Spleen
Tyrosine Kinase Inhibitor Blocks Fc Receptor Signaling and Reduces
Immune Complex-Mediated Inflammation. Journal of Pharmacology
and Experimental Therapeutics, 319, 998-1008.
http://www.allcells.com/publications/0101allcells.pdf
www.allcells.com
For In Vitro Research Use Only
56
Normal Peripheral Blood—Monocyte—Derived Myeloid Dendritic Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
PB-DC001F
1.5 million/vial
Inquire
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Monocyte-Derived Myeloid Dendritic Cells
•Purity
Product Description
NPB-Monocyte Derived Myeloid Dendritic Cells (MoDC) are
derived from highly purified monocytes. For six to seven days,
the highly purified monocytes are cultured with 10% Fetal
Bovine Serum in the presence of GM-CSF and IL-4. MoDC
have a longer lifespan in vitro when kept inactivated, which
allows them to have a longer storage in culture.
Application
These cells express the following markers: CD11c, CD40,
and CD86 (>90%). These cells lack the expression of CD14
(<10%).
Lead Time
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Monocyte Derived Myeloid DC can be used to study:
• Antigenic endocytosis, processing, and presentation
mechanism and pathway
• DC-SIGN receptor expression and its relation to HIV
infection
• Toll-like receptors and other pattern recognition receptors
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Normal Peripheral Blood
Cell Products
57
Normal Peripheral Blood
Normal Peripheral Blood—Monocyte-Derived Macrophages
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
PB-MDM-001F
1.5 million/vial
Inquire
Macrophage Basal Medium
PB-MDM-002
450 ml
$63
Macrophage Stimulatory
Supplement
PB-MDM-003
50 ml
$399
Media
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
NPB-Monocyte-Derived Macrophages
Product Description
Product Description
NPB-Macrophages are derived from highly purified
peripheral blood monocytes and stimulated with M-CSF
and IL-4 growth factors and cultured for 6-7 days.
Application
Monocyte-Derived Macrophages can be used to study
the following:
• Inflammation
• Antigen presentation
• T-Cell activation
• Phagocytosis pathways
• Atherosclerosis
• HIV infection
• Toll-like receptors and other pattern recognition
receptors
This product contains L-glutamine, 110mg/L sodium pyruvate,
and pyridoxine hydrochloride. This product does not contain
growth factors and antibiotics. pH >7.1 to <7.5. The Macrophage Basal Medium should be used in combination with
AllCells Macrophage Stimulatory Supplement. This product is
stable for one month after the stimulatory supplement has been
added and stored at 2 to 8°C.
Macrophage Stimulatory Supplement
Product Description
Macrophage Stimulatory Supplement is designed to initiate and
maintain growth of AllCells Monocyte Derived Macrophages.
This product contains pre-selected Fetal Bovine Serum
and supplements.
Lead Time
Purity
These cells have phagocytic properties and express the
following markers: MHC class II (>80%), CD163 (>80%),
CD18 (>80%) and CD32 (>80%).
ALLCELLS
Macrophage Basal Medium
Toll Free Phone: 1-888-535-3444
1-2 business days for frozen products. Product delivery is subject to stock availability.
www.allcells.com
For In Vitro Research Use Only
58
MOBILIZED PERIPHERAL BLOOD
Mobilized Peripheral Blood Table of Contents
Apheresis Collection (Leuko Pak)
59
Stem/Progenitor Cells - CD133+ 63
Mononuclear Cells (MNC)
60
Monocytes - CD14+64
CD34 Depleted Mononuclear Cells
61
Stem/Progenitor Cells - CD34+ 62
Mobilized Peripheral Blood
AllCells has been a pioneer in the use of G-CSF (Granulocyte-Colony Stimulating Factor) stimulation in normal healthy donors since
1998. The protocols in use for our Mobilized Peripheral Blood (MPB) products have been approved by an IRB and are overseen by clinical
physicians. In addition AllCells offers Plerixafor (Mozobil®) or a combination of Plerixafor (Mozobil®) and Neupogen mobilization of stem cells
from healthy donors. Mozobil, a selective CXCR4 antagonist, has been particularly beneficial as an adjuvant stem cell mobilization agent on top of
the G-CSF regimen. The Mozobil regimen requires only 1 injection as opposed to the G-CSF 3-5 day protocol.
All donors are monitored during the injection, collection and post-collection procedures to ensure the health of our donors is a priority.
AllCells is one of only a few providers of Mobilized Peripheral Blood in the world.
Please specify which mobilization factor you prefer when ordering.
Background
Granulocyte-Colony Stimulating Factor (G-CSF, Neupogen®) can stimulate bone marrow to produce a large number of hematopoietic
stem/progenitor cells. G-CSF can mobilize these stem/progenitor cells from bone marrow into the peripheral blood stream. The effects
of G-CSF have changed the practice of bone marrow transplantation. Consequently, mobilized peripheral blood cells (MPB) have become
major sources for clinical hematopoietic stem cell transplantation.
Donor Criteria
•
Age 18-40 years old
•
Donors must be in good health
•
Weight >140 lbs for Leuko Pak
•
Donors are required to sign consent form prior to collection
•
Complete Blood Count lab test must meet protocol specs
•
All consent and protocols approved by an IRB
•
Donors cannot be pregnant
•
Minimum 12 months between donations
•
Donors are screened for HIV, Hepatitis B & C
•
Twice-in-a-lifetime maximum donation
For more information visit: www.allcells.com/mobilized-peripheral-blood/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
59
Mobilized Peripheral Blood
Mobilized Peripheral Blood—Apheresis Collection—Leuko Pak
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh−Leuko Pak (One-day)
mPB025-Moz.A
1 Bag
$35378
Fresh−Leuko Pak (One-day)
mPB025-Reg.G
1 Bag
$13194
Fresh−Leuko Pak (Two-day)
mPB026-Reg.F
2 Bags
$19499
Fresh−Leuko Pak (Two-day)
mPB026-Reg.H
2 Bags
$52779
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Mobilized Peripheral Blood (MPB)-Leuko Pak
Application
Product Description
One-Day Mozobil MPB-Leuko Pak- This product is obtained by a 20 mg/ml, 1.2 ml single dose of Mozobil and apheresis 9-11 hours post injection.
One-Day G-CSF MPB-Leuko Pak- This product is obtained
by 5.0-9.6 µg/kg/day of G-CSF injections for 3-5 days and
apheresis on Day 5 (see regimen table below).
MPB-Leuko paks can be used to:
• Study post-stimulation effects of Neupogen® (G-CSF)
or Mozobil® (Plerixafor) on general profiles and
individual MNC phenotypes.
• Enrich CD34+ human stem cells for further culture
studies.
Lead Time
Related Products
A minimum
ofPak
four weeks is needed.
NPB-Leuko
Leuko Pak Cell Number Ranges
MNC Avg. Cell Number: 2.3x1010 Range: 0.9 to 5.6x1010
CD34+ Avg. Cell Number: 1.86x108 Range: 0.6 to 4.8x108
Two-Day G-CSF MPB-Leuko Pak- This product is obtained
by 6.9 µg - 9.0 µg/kg/day (depending on the donor’s weight) of
G-CSF injections for 5 days and apheresis on Day 5 and Day 6
(1 bag delivery per day).
Two-Day G-CSF MPB-Leuko Pak- This product is obtained
by 6.9 µg - 9.0 µg/kg/day (depending on the donor’s weight) of
G-CSF injections for 5 days and apheresis on Day 5 and Day 6
(1 bag delivery per day).
Leuko Pak Cell Number Ranges
MNC Avg. Cell Number: 6.0x1010 Range: 1.2 to 12.4x1010
CD34+ Avg. Cell Number: 4.5x108 Range: 0.9 to 8.1x10 8
Published Papers
Chy-Anh Tran, et al. (2012). Optimized Processing of Growth Factor
Mobilized Peripheral Blood CD34 Products by Counterflow Centrifugal
Elutriation. Stem Cells Translational Medicine, 1(5) http://www.allcells.
com/publications/0144allcells.pdf
Campbell-Anson, Ryan E., et al. (2008). A new approach for the largescale generation of mature dendritic cells from adherent PBMC using
roller bottle technology. Journal of Immune Based Therapies and Vaccines, 6(1) http://www.allcells.com/publications/0003allcells.pdf
Mahmood, Kutubuddin, et al. (2005). Human cytomegalovirus plasmidbased amplicon vector system for gene therapy. Genetic Vaccines
Therapy, 3, 1. Retrieved June 20, 2008,
http://www.allcells.com/publications/0075allcells.pdf
Hoffman, Joseph F., et al. (2002). Na pump isoforms in human
erythroid progenitor cells and mature erythrocytes. PNAS, 99(22),
4572–14577.
Mahmud, Dolores L., et al. (2002). Phosphorylation of forkhead transcription factors by erythropoietin and stem cell factor prevents acetylation and their interaction with coactivator p300 in erythroid progenitor
cells. Oncogene, 21, 1556–1562.
http://www.allcells.com/publications/0098allcells.pdf
Product
Regimen
Qty
mPB025-Moz.A
Mozobil @240 µg/kg/day x 1 day - Regimen A, apheresis 9-11 hours post injection
1 bag
mPB025-Reg.G
G-CSF @10 µg/kg/day x 5 days - Regimen G, apheresis on day 6
1 bag
mPB026-Reg.F
G-CSF @10 µg/kg/day x 5 days - Regimen F, apheresis on days 5 and 6 (2 bags)
2 bags
mPB026-Reg.H
G-CSF @10 µg/kg/day x 5 days; Mozobil @240 µg/kg/day x 2 days (day 4 and 5) Regimen H, apheresis on days 5 and 6 (2 bags)
2 bags
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
60
Mobilized Peripheral Blood—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB001
150 million/vial
$699
mPB002
250 million/vial
$829
mPB003
350 million/vial
$969
mPB004F
5 million/vial
$141
mPB005F
15 million/vial
$181
mPB006F
25 million/vial
$279
mPB007F
50 million/vial
$419
mPB008F
100 million/vial
$569
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-Mononuclear Cells
Product Description
These mononuclear cells (MNC) are collected using the COBE
Spectra™ apheresis machine (>90% of the cells are MNC).
These MPB-MNC products have been depleted of red blood
cells and platelets.
Lead Time
MPB Cell Subsets
Average Percentage of Cell
Subsets
CD34+
0.8%
CD133
0.6%
CD3+
33%
CD4
18%
CD8+
6%
+
+
CD14+
25%
CD16
16%
CD19+
5%
CD33
35%
CD56+
6%
CD15+
0.11%
+
A minimum of four weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
NPB-MNC
Published Papers
Lund, Troy C., et al. (2008). Granulocyte colony-stimulating factor mobilized CFU-F can be found in the peripheral blood but have limited
expansion potential. haematologica, 93(6)
http://www.allcells.com/publication/0068allcells.pdf
+
The table above is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and not
the total white blood cell population. We cannot guarantee the recovery of purification products listed above. For a specific number of a certain cell type, please
consider our mobilized peripheral blood purified products.
Mahmud, Dolores L., et al. (2002). Phosphorylation of forkhead
transcription factors by erythropoietin and stem cell factor prevents
acetylation and their interaction with coactivator p300 in erythroid
progenitor cells. Oncogene, 21, 1556–1562.
http://www.allcells.com/publications/0098allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Mobilized Peripheral Blood
Cell Products
61
Mobilized Peripheral Blood
Mobilized Peripheral Blood—CD34 Depleted Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
mPB021F
100 million/vial
$169
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD34-Depleted MNC
Product Description
Lead Time
MPB-CD34 Depleted MNC are isolated using
immunomagnetic CD34 MicroBead labeling system to deplete
the CD34+ cell population. This product contains only a
minimal amount of CD34 + cells.
Application
MPB-CD34 Depleted MNC can be used to:
• Serve as a cost-effective alternative source of
hematopoietic mononuclear cells, excluding the stem/
progenitor cell population.
• Study the cell population profile changes within the
mononuclear cells after G-CSF simulation.
• Research drugs that suppress GVHD for transfusion
or transplantation purposes.
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of four weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
BM-CD34 Depleted MNC
CB-CD34 Depleted MNC
www.allcells.com
For In Vitro Research Use Only
62
Mobilized Peripheral Blood—Stem/Progenitor Cells—CD34 + Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB009
1 million/vial
$839
mPB010
5 million/vial
$3299
mPB011
10 million/vial
$5619
mPB012
20 million/vial
$7999
mPB013
30 million/vial
$8399
mPB014F
0.2 million/vial
$274
mPB015F
1 million/vial
$689
mPB016F
5 million/vial
$2669
mPB017F
10 million/vial
$3929
mPB018F
20 million/vial
$5399
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD34+ Stem/Progenitor Cells
Product Description
MPB-CD34+ Stem/Progenitor cells are positively selected
using a direct immunomagnetic CD34 MicroBead labeling
system.
Purity
Published Papers
Zhu, Xingguo, et al. (2012). NF-Y Recruits Both Transcription Activator and Repressor to Modulate Tissue- and Developmental StageSpecific Expression of Human c-Globin Gene. PLoS ONE, 7(10)
http://www.allcells.com/publications/0172allcells.pdf
MacEneaney, Owen J., et al. (2011). Effects of HIV-1 GP120 and
Protease Inhibitors on Apoptotic Susceptibility of CD34+ Hematopoietic Progenitor Cells. J Acquir Immune Defic Syndr., 56(2), 49–50.
http://www.allcells.com/publications/0151allcells.pdf
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of four weeks is needed for fresh cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Mobilized Peripheral Blood
Cell Products
63
Mobilized Peripheral Blood—Stem/Progenitor Cells—CD133 + Cells
Mobilized Peripheral Blood
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB023-1
0.5 million/vial
$999
mPB023-2
2.5 million/vial
$3029
mPB023-3
5 million/vial
$5549
mPB023-4
10 million/vial
$8249
mPB023-5
20 million/vial
$11099
mPB024F-1
0.5 million/vial
$759
mPB024F-2
2.5 million/vial
$2909
mPB024F-3
5 million/vial
$5139
mPB024F-4
10 million/vial
$7649
mPB024F-5
20 million/vial
$10749
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD133+ Stem/Progenitor Cells
Purity
Product Description
MPB-CD133+ Stem/Progenitor cells are positively isolated
from MPB-MNC by using a direct immunomagnetic CD133
MicroBeads labeling system. The majority of CD133+ Stem/
Progenitor cells also express the CD34 cell marker.
Lead Time
A minimum of four weeks is needed. Product delivery is subject
to stock availability.
Application
MPB-CD133+ cells can be used to study expansion and differentiation capacity as nascent stem cells. MPB-CD34+ cells
have the highest frequency of CD133 double-positive cells
when compared to bone marrow and cord blood.
ALLCELLS
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
Published Papers
Bissels, Ute, et al. (2011). Combined Characterization of microRNA
and mRNA Profiles Delineates Early Differentiation Pathways of
CD133+ and CD34+ Hematopoietic Stem and Progenitor Cells. STEM
CELLS, 29(5). http://www.allcells.com/publications/0147allcells.pdf
www.allcells.com
For In Vitro Research Use Only
64
Mobilized Peripheral Blood—CD14 + Monocytes
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
mPB033-1
15 million/vial
$1059
mPB033-2
25 million/vial
$1179
mPB033-3
45 million/vial
$1619
mPB033F-1
15 million/vial
$1039
mPB033F-2
25 million/vial
$1129
mPB033F-3
45 million/vial
$1369
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MPB-CD14+ Monocytes
Product Description
Purity
MPB-CD14+ Monocytes are positively isolated from MPB-MNC
using a direct immunomagnetic CD14 MicroBeads labeling
system.
Application
MPB-Monocytes can be used to study:
• Down-regulation of T cells for allogeneic transplantation
• The impact of G-CSF on the antigen-presenting and
macrophage-forming abilities of monocytes
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
A minimum of four weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
CB-Monocytes
www.allcells.com
NPB-Monocytes
For In Vitro Research Use Only
Mobilized Peripheral Blood
Cell Products
65
BONE MARROW
Bone Marrow Table of Contents
Aspirates (Whole Bone Marrow)
66
Early Erythroid Progenitor Cells - CD36+
70
Mononuclear Cells (MNC)
67
Endothelial Cells - CD105 71
CD34 Depleted Mononuclear Cells
68
Mesenchymal Stem Cells
+
72
Stem/Progenitor Cells -CD34 69
+
Bone Marrow Cells and Tissue
AllCells is able to provide whole bone marrow aspirate and bone marrow cells collected from healthy individuals. These bone
marrow products are available in fresh or frozen format.
Background
Bone Marrow (BM) contains hematopoietic stem/progenitor cells, which are self-renewing, proliferating, and differentiating
into multi-lineage blood cells. Multipotent, non-hematopoietic stem cells, such as bone marrow mesenchymal stem cells
(MSC), can be isolated from human bone marrow as well. These non-hematopoietic, bone marrow MSCs are capable of both
self-renewal and differentiation into bone, cartilage, muscle, tendons, and fat. 100 mL of bone marrow cells and tissue is
drawn into a 20 cc syringe x 12 tubes containing heparin (80 U/mL of BM) from the posterior iliac crest, at a maximum of
eight separate sites.
Donor Criteria
•
Age: 18-65 years old
•
Donors cannot be pregnant.
•
Weight: >110 lbs for 25mL and 50mL donations
•
Donors are screened for HIV, Hepatitis B and Hepatitis C
•
Weight: >130 lbs for 100mL donations
•
CBC lab tests must meet protocol specifications.
•
Donors must be in general good health. Donors with any of the
following will be excluded from donating:
–History of heart, lung, liver, kidney disease (including asthma)
–Blood and bleeding disorders, including sickle cell disease
–Neurologic disorders
before donation.
•
Donors are required to sign a procedure-specific consent form
prior to the collection procedure.
•
All consents and protocols have been approved by an
independent biomedical review board.
–Cancer
–Diabetes
–Positive: HIV, HbsAg, HCAb
For more information visit: www.allcells.com/bone-marrow/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
66
Bone Marrow—Aspirates—Bone Marrow (Whole)
Product Format
Fresh
Catalog #
QTY/Unit
Price/Unit
ABM001-1
3 ml
$251
ABM001
10 ml
$419
ABM002
25 ml
$909
ABM003
50 ml
$1769
ABM004
100 ml
$3189
HLA-Typed Products are available upon request.
Bone Marrow
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-Whole
Product Description
Lead Time
Bone Marrow (BM) contains hematopoietic stem/progenitor
cells, which have the potential to self-renew, proliferate, and
differentiate into multi-lineage blood cells. Multipotent, nonhematopoietic stem cells, such as mesenchymal stem cells, can
be isolated from human BM as well. These non-hematopoietic,
mesenchymal stem cells are capable of both self-renewal and
differentiation into bone, cartilage, muscle, tendons, and fat.
BM is drawn into a 20 cc syringe containing heparin (80 U/
mL of BM) from the posterior iliac crest, 25mL/site, from a
maximum of four sites.
Application
BM can be used to:
• Serve as a source for hematopoietic progenitor cells, mesenchymal stem cells, and other cell types, such as CD33+
myeloid cells and CD138 + plasma cells.
• Study hematopoietic cell differentiation pathways.
• Act as controls for BM malignancies studies.
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells.
Related Products
Diseased Bone Marrow Cells
Published Papers
Oskouei, Behzad N., et al. (2012) Increased Potency of Cardiac
Stem Cells Compared with Bone Marrow Mesenchymal Stem Cells
in Cardiac Repair. Stem Cells Translational Medicine, 1(2) 116124. http://www.allcells.com/publications/0143allcells.pdf
Yamaza, et al. (2010) Immunomodulatory properties of stem cells
from human exfoliated deciduous teeth. Stem Cell Research &
Therapy, 1(5).
http://www.allcells.com/publications/0009allcells.pdf
www.allcells.com
For In Vitro Research Use Only
67
Bone Marrow—Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM024
25 million/vial
$389
Frozen
ABM007F
5 million/vial
$155
ABM008F
15 million/vial
$289
ABM009F
25 million/vial
$389
HLA-Typed Products are available upon request.
Bone Marrow
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-MNC
BM Cell Subset Products
Product Description
BM-MNC are isolated by diluting bone marrow with PBS
and using density gradient separation techniques. After
centrifugation, the MNC layer is collected. MNC can be
processed further to isolate various subpopulations.
The table below is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and not
the total white blood cell population.
BM Cell Subsets
Average Percentage of
Cell Subsets
CD34+
2.5%
CD133
1.1%
CD33+
45%
+
Published Papers
Novotny-Diermayr, V., et al. (2012) The oral HDAC inhibitor pracinostat (SB939) is efficacious and synergistic with the JAK2 inhibitor
pacritinib (SB1518) in preclinical models of AML. Blood Cancer
Journal, 2, e69. http://www.allcells.com/publications/0159allcells.pdf
Gao, N, et al. (2012) 3, 39-Diindolylmethane Exhibits Antileukemic
Activity In Vitro and In Vivo through a Akt-Dependent Process. PLoS
ONE, 7(2). http://www.allcells.com/publications/0141allcells.pdf
Majeti, Ravindra, et al. (2009) Dysregulated gene expression
networks in human acute myelogenous leukemia stem cells. PNAS,
106(9). http://www.allcells.com/publications/0060allcells.pdf
Seo, BM, et al. (2008) SHED repair critical-size calvarial defects in
mice. Oral Dis. 14(5).
http://www.allcells.com/publications/0107allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
CD105
0.7%
CD19+
24%
CD14
7.8%
CD3+
34%
Granulocytes
10%
RBC
<0.01%
Platelets
<0.01%
CD138+ Plasma Cells
0.1-0.4%
+
+
We cannot guarantee the recovery of the cell types listed above. For a specific
number of a certain cell type, please consider our bone marrow purified products.
www.allcells.com
For In Vitro Research Use Only
68
Bone Marrow—CD34 Depleted Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ABM023F
100 million/vial
$359
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Lead Time
Product Description
BM-CD34 Depleted MNC are isolated using a direct
immunomagnetic CD34 MicroBead labeling system to deplete
CD34+ cells. This product contains only a minimal amount of
BM-CD34 + cells.
1-2 business days for frozen products. Product delivery is
subject to stock availability.
Related Products
Application
CB CD34-Depleted MNC
BM-CD34 Depleted MNC can be used to compare CFU-GM
output with CD34+.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Bone Marrow
BM-CD34 Depleted MNC
69
Bone Marrow—Stem/Progenitor Cells—CD34 + Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM009
0.1 million/vial
$249
ABM010
0.3 million/vial
$459
ABM011
0.5 million/vial
$699
ABM012
1 million/vial
$1009
ABM013
5 million/vial
$4849
Bone Marrow
Frozen
ABM015F
0.1 million/vial
$241
ABM021F
0.3 million/vial
$411
ABM022F
0.5 million/vial
$589
ABM017F
1 million/vial
$949
ABM018F
5 million/vial
$4579
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-CD34+ Stem/Progenitor Cells
Product Description
BM-CD34+ stem/progenitor cells are positively isolated using a
direct immunomagnetic CD34 MicroBead labeling system.
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
BM-CD34+ cells can be used for the following:
• Study of hematopoiesis and disease pathways.
• Act as a control for leukemia and leukemia stem
cell studies.
• Facilitate colony-forming and stem cell toxicity assays.
• Research in vitro stem cell cloning
Purity
The purity is guaranteed to be at least 90% as
assessed by flow cytometric analysis.
Related Products
CB-CD34+ Cells
MPB-CD34+ Cells
NPB-CD34+ Cells
Published Papers
Cokic, Vladan P., et al. (2012) JAK-STAT and AKT pathway-coupled
genes in erythroid progenitor cells through ontogeny. Journal of
Translational Medicine, 10 (116).
http://www.allcells.com/publications/0157allcells.pdf
Karp, Judith E., et al. (2011) Phase 1 and pharmacokinetic study
of bolus-infusion flavopiridol followed by cytosine arabinoside and
mitoxantrone for acute leukemias. Blood, 117 (12).
http://www.allcells.com/publications/0168allcells.pdf
Huang, Xinqiang., et al. (2011) Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem
cells. Blood, 117 (7).
http://www.allcells.com/publications/0167allcells.pdf
Nahimana, A., et al. (2011) A novel potent Fas agonist for selective
depletion of tumor cells in hematopoietic transplants. Blood Cancer
Journal, 1 (e47).
http://www.allcells.com/publications/0150allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
70
Bone Marrow—CD36 + Early Erythroid Progenitor Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ABM020F
1 million/vial
$671
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Lead Time
BM-CD36+ Early Erythroid Progenitor cells are derived from
BM-CD34 + cells in culture. The BM-CD34+ cells are cultured
for 7 to 8 days in serum free media with the addition of SCF,
EPO, IL-3 and IL-6 growth factors. The cells are harvested from
culture, and the BM-CD36+ cells are positively isolated, using
direct immunomagnetic CD36 MicroBeads labeling system.
Application
BM-CD36+ cells can be used to:
• Study the parvovirus B19 tropism for erythroid cells
• Replace the traditional CD71 + cells as an immediate and
abundant source for erythroid cell studies and
transplantation studies
• Research erythropoiesis.
• Serve as a control in therapeutic studies for early
erythroblastic leukemia and polycythemia vera (PV)
Product delivery is subject to stock availability.
Related Products
CB-CD36+ Cells
MPB-CD36+ Cells
Published Papers
Mulligan, George, et al. (2007). Gene expression profiling and correlation with outcome in clinical trials of the proteasome inhibitor
bortezomib. Blood, 109(8), 3177-3188.
http://www.allcells.com/publications/0065allcells.pdf
Purity
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Bone Marrow
BM-CD36+ Early Erythroid Progenitor Cells
71
Bone Marrow—CD105 + Endothelial Cells
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
ABM028
0.3 million/vial
$951
Frozen
ABM028F
0.3 million/vial
$939
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Bone Marrow
BM-CD105+ Endothelial Cells
Product Description
Purity
BM-CD105 Endothelial Cells are positively selected from
bone marrow MNC, using a direct immunomagnetic CD105
MicroBeads labeling system.
+
BM-CD105+ Endothelial cells express endoglin, a type III
receptor of the transforming growth factor-beta (TGF-ß)
receptor family. These cells have the ability for multiplelineage differentiation. After 14 days of culture with
Alpha-MEM + 10% FBS + 1% antimycotic-antibotic, there is
a four-fold expansion of BM-CD105+ Endothelial cells. These
cultured cells express the following cell markers: CD105,
CD13, CD29, CD44, CD49b, CD49e, CD54, CD71, CD90. The
aforementioned markers are similar to the markers expressed
on BM mesenchymal stem cells.
The purity is guaranteed to be at least 80% as assessed by
flow cytometric analysis.
Lead Time
A minimum of two weeks is needed for fresh cells.
Application
BM-CD105+ Endothelial Cells can be used to study:
• Angiogenesis
• Anti-angiogenesis in tumor therapy research
• Selective multi-potency
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
72
Bone Marrow—Mesenchymal Stem Cells
Cell Products
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MSC-001F
0.75 million/vial
$841
HLA-Typed Products are available upon request.
Media
MSC-002
500 ml
$76
MSC Stimulatory Supplement
MSC-003
50 ml
$263
Bone Marrow
MSC Basal Medium
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
BM-Mesenchymal Stem Cells
Product Description
MSC Basal Medium
Product Description
Bone Marrow Mesenchymal Stem Cells (MSC) are derived
from BM-MNC from the first culture passage.
Application
BM-MSC can be used to:
• Study non-hematopoietic pluripotency potentials and
characteristics to develop connective tissues, such as
bone and cartilage
• Act as stroma and feeder cells for expansion or
differentiation of hematopoietic stem and progenitor cells
Mesenchymal Stem Cell Basal Medium is designed to support
AllCells BM-MSC. MSC Basal medium contains L-glutamine,
110mg/L sodium pyruvate, and pyridoxine hydrochloride. This
product does not contain growth factors and antibiotics. The
pH range is >7.1 to <7.5. MSC Basal Medium should be used
in combination with AllCells MSC Stimulatory Supplement.
This product is stable for one month after the stimulatory
supplement has been added and stored at 2 to 8°C.
MSC Stimulatory Supplement
Product Description
Purity
These BM-MSC have fibroblast-like morphology and express
CD105+ (>90%), CD73+(>90%) and CD90+ (>90%). MSC
lack expression of CD34, CD11b and CD45.
Lead Time
1-2 business days for frozen products. Product delivery is subject to stock availability.
Mesenchymal Stem Cell Stimulatory Supplement is designed to
initiate and maintain growth of AllCells BM-MSC. This product
contains pre-selected Fetal Bovine Serum and supplements.
Published Papers
Fukushima, H., et al. (2012) SSEA-4 is a Marker of Human Deciduous
Periodontal Ligament Stem Cells. Journal of Dental Research, August
14, 2012. http://www.allcells.com/publications/0152allcells.pdf
Milanesi, Anna, et al. (2012) b-Cell Regeneration Mediated by Human
Bone Marrow Mesenchymal Stem Cells. PLoS ONE, 7(8).
http://www.allcells.com/publications/0142allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
73
CORD BLOOD
Cord Blood Table of Contents
Plasma74
CD19+ B Cells80
Mononuclear Cells (MNC)75
CD3+ Pan T Cells81
CD34 Depleted Mononuclear Cells
CD4+ Helper T Cells82
76
Stem/Progenitor Cells - CD34+ 77
CD8+ Cytotoxic T Cells
Early Erythroid Progenitor Cells - CD36 78
CD4 /CD45RA Naive T Cells
84
CD14+ Monocytes79
CD56+ Natural Killer Cells
85
+
+
+
83
Cord Blood Cells & Tissue
AllCells is able to provide cord blood cells from healthy individuals. These include umbilical cord stem cells, cord blood mononuclear cells,
and additional products. These cord blood products are available in fresh, frozen, lysate, or cell pellet format. AllCells is able to extract
molecular products including Comprehensive RNA and cDNA for your research needs.
Cells can be pulled from mixed or single donors. When ordering please specify preference.
Donor Criteria
•
Donors are at least 18 years old.
•
Donors are required to sign a procedure-specific consent form prior to the collection procedure.
•
All consents and protocols have been approved by an independent biomedical review board.
Background
Cord blood (CB) is collected at the time of delivery in a 250 mL standard blood bag containing citrate-phosphate dextrose (CPD)
anticoagulant (35mL/bag). The cord blood is processed within 24-48 hours of collection.
For more information visit: www.allcells.com/cord-blood/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
74
Cord Blood—Plasma
Fresh
Frozen
Catalog #
QTY/Unit
Price/Unit
CB027
10 ml
$109
CB028
20 ml
$169
CB029
30 ml
$189
CB030
40 ml
$229
CB031
50 ml
$251
CB027F
10 ml
$89
CB028F
20 ml
$129
CB029F
30 ml
$169
CB030F
40 ml
$211
CB031F
50 ml
$229
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-Plasma
Product Description
Lead Time
CB-Plasma is obtained by centrifugation of whole cord blood
tissue. This product has a diminished number of platelets.
A minimum of 1-2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Application
Related Products
NPB Plasma
CB-Plasma can be used to study the following:
• Neonatal platelets, cytokines, and immunoglobulins
• Free-radical trapping ability
• Beneficial effects of cord blood plasma for cord blood
stem cell transplantation and increased efficiency
during gene transfection
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Cord Blood
Product Format
75
Cord Blood—Mononuclear Cells (MNC)
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CB002F
15 million/vial
$149
CB004F
50 million/vial
$599
CB003F
150 million/vial
$899
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-MNC
Cord Blood
Product Description
CB-Mononuclear Cells (MNC) are isolated by diluting the CB
with phosphate-buffered saline and using density gradient
separation techniques. After centrifugation, the MNC layer is
collected. MNC can be processed to isolate subpopulations.
Application
CB-MNC can be used to study:
• Mesenchymal stem/progenitor cell expansion potential
• Stimulation of neuro-regeneration in the hippocampus
• Sources of endothelial progenitor cells and
neovascularization
• Possible transplantation sources for HIV or SCID diseases
• Remedies for nuclear and radioactive damage
Lead Time
A minimum of 1-2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Cell Subsets in CB
Average Percentage of
(Recoverable) Cell Subsets
CD34+
2.5%
CD133+
4.1%
CD19
45%
CD14+
6%
+
CD3
0.7%
CD4+
24%
CD8
7.8%
+
+
CD56+
34%
CD4 /CD45RA
+
+
11%
Granulocytes
10%
RBC
<0.01%
Platelets
<0.01%
The table on the top is for reference purposes only. Please note that the average
percentage of cell subsets is referring to the recovered MNC population and
not the total white blood cell population. We cannot guarantee the recovery of
purification products listed above. For a specific number of a certain cell type,
please consider our cord blood purified products.
Related Products
BM-MNC
MPB-MNC
NPB-MNC
Published Papers
Medina, Reinhold J, et al. (2010) Molecular analysis of endothelial
progenitor cell (EPC) subtypes reveals two distinct cell populations
with different identities. BMC Medical Genomics, 3 (18).
http://www.allcells.com/publications/0010allcells.pdf
Yang, Li, et al. (2008) IL-21 and TGF-B are required for differentiation
of human TH17 cells. Nature, 454(7202).
http://www.allcells.com/publications/0078allcells.pdf
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
76
Cord Blood—CD34 Depleted Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen (Single)
CB011F
100 million/vial
$349
Frozen (Mixed)
CB011F-M
100 million/vial
$349
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD34 Depleted MNC
Product Description
Lead Time
CB-CD34 Depleted MNC/Mixed indicates that multiple donors
are pooled to obtain the cord blood mononuclear cells.
CB-CD34 Depleted MNC/Single indicates that only one donor
is used to obtain the cord blood mononuclear cells.
A minimum of 1-2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Cord Blood
CB CD34 Depleted MNC are isolated, using a direct
immunomagnetic CD34 MicroBead labeling system to deplete
the CD34+ cells. This product contains only a minimal amount
of CD34 + cells.
Related Products
CB-MNC
CB-CD34+ Cells
Application
CB-CD34 Depleted MNC can be used to research endothelial
cell and mesenchymal stem cell development.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
77
Cord Blood—Stem/Progenitor Cells—CD34 + Cells
Mixed Donors/Cell Products
Product Format
Fresh
Frozen
Catalog #
QTY/Unit
Price/Unit
CB005
1 million/vial
$1209
CB007F
0.2 million/vial
$444
CB005F
0.5 million/vial
$759
CB008F
1 million/vial
$1149
CB009F
5 million/vial
$4049
CB007F-S
0.2 million/vial
$579
CB005F-S
0.5 million/vial
$999
CB008F-S
1 million/vial
$1799
Single Donor/Cell Products
Cord Blood
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD34+ Stem/Progenitor Cells
Product Description
CB-CD34+ Stem/Progenitor cells are positively isolated using
a direct immunomagnetic CD34 MicroBead labeling system.
CB-CD34 + Cells/Mixed indicates that multiple donors are
pooled to obtain the cord blood CD34 + cells.
CB-CD34 Cells/Single indicates that only one donor is used
to obtain the cord blood CD34+ cells.
+
Related Products
NPB-CD34+
BMCD34+
MPBCD34+
Published Papers
Wang T., et al. (2013) Derivation of Neural Stem Cells from Human
Adult Peripheral CD34+ Cells for an Autologous Model of Neuroinflammation. PLoS ONE 8(11).
http://www.allcells.com/publications/0206allcells.pdf
Cokic, Vladan P., et al. (2012) JAK-STAT and AKT pathway-coupled
genes in erythroid progenitor cells through ontogeny. Journal of
Translational Medicine, 10 (116).
http://www.allcells.com/publications/0157allcells.pdf
Park, Tea Soon, et al. (2012) Growth Factor-Activated Stem Cell
Circuits and Stromal Signals Cooperatively Accelerate Non-Integrated
iPSC Reprogramming of Human Myeloid Progenitorss. PLoS ONE
7(8). http://www.allcells.com/publications/0128allcells.pdf
Application
CB-CD34+ cells can be used to study the following:
• Hematopoiesis
• Stem cell expansion and self-renewal
• Transplantation research for hematopoietic malignancies
Purity
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
78
Cord Blood—CD36 + Early Erythroid Progenitor Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CB010M-F
1 million/vial
$691
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD36+ Early Erythroid Progenitor Cells
Purity
CB-CD36+ Early Erythroid Progenitor cells are derived from
CB-CD34 + cells in culture. CB-CD34+ cells are cultured
for seven to eight days using StemSpan SFEM (StemCell
Technologies, Inc.), a serum free expansion media for
hematopoietic cells, EPO, SCF, IL-3, and IL-6 growth factors.
The cells are harvested from culture, and the CB-CD36+ cells
are positively isolated using a direct immunomagnetic CD36
MicroBead labeling system.
Application
CB-CD36+ cells can be used to:
• Study the parvovirus B19 tropism for erythroid cells.
• Replace the traditional CD71 + cells as an immediate
and abundant source for erythroid cell studies and
transplantation studies.
• Research erythropoiesis.
• Act as a control in therapeutic studies for early
erythroblastic leukemia and polycythemia vera (PV).
• Study production and functions of hemoglobin.
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
1-2 business days for frozen products. Product delivery is
subject to stock availability.
Related Products
BM-CD36+ Cells
MPB-CD36+ Cells
www.allcells.com
For In Vitro Research Use Only
Cord Blood
Product Description
79
Cord Blood—CD14 + Monocytes
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB022
5 million/vial
$1399
Frozen
CB022F
5 million/vial
$1199
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD14+ Monocytes
Purity
Product Description
Cord Blood
CB CD14+ Monocytes are positively selected from
mononuclear cells, using immunomagnetic CD14 MicroBeads.
Lead Time
Application
CB-CD14+ Monocytes can be used to study the following:
• Antigen presentation and phagocytic property
• Induced macrophage differentiation
• Dendritic cell differentiation
• Osteoclast differentiation
• HIV infection mechanisms
• Fc receptor binding and antibody research
ALLCELLS
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
A minimum of two weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-Monocytes
NPB-Monocytes
www.allcells.com
For In Vitro Research Use Only
80
Cord Blood—CD19 + B Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB021
5 million/vial
$1399
Frozen
CB019F
1 million/vial
$499
CB020F
2.5 million/vial
$799
CB021F
5 million/vial
$1299
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Purity
CB-CD19+ B cells are positively isolated from mononuclear
cells, using a direct immunomagnetic CD19 MicroBeads
labeling system.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
Application
CD19 B Cells can be used to study:
• Naïve B cell expansion
• T Cell regulation of immuno-activity
• Gene rearrangement of immunoglobulins
+
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of 2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-B Cells
NPB-B Cells
www.allcells.com
For In Vitro Research Use Only
Cord Blood
CB-CD19+ B Cells
81
Cord Blood—T Cells—CD3 + Pan T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-1
15 million/vial
$1199
Frozen
CB020-1F
15 million/vial
$999
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD3+ Pan T Cells
Cord Blood
Product Description
Purity
CB-Pan T cells are negatively isolated from mononuclear cells,
using an indirect immunomagnetic Pan T labeling system.
Application
Lead Time
Pan T cells can be used to:
• Study immunosuppression of GVHD in transplantation
experiments
• Research their ability to generate anti-viral cytotoxic T
cells without prior (prenatal) exposure to viral antigens
• Act as a control for childhood lymphocytic
leukemia studies
ALLCELLS
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Toll Free Phone: 1-888-535-3444
A minimum of 2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-Pan T Cells
NPB-Pan T Cells
www.allcells.com
For In Vitro Research Use Only
82
Cord Blood— T Cells—CD4 + Helper T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-2
15 million/vial
$1299
Frozen
CB020-2F
15 million/vial
$999
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD4+ Helper T Cells
Cord Blood CD4+ Helper T cells are negatively isolated from
mononuclear cells using an indirect immunomagnetic CD4+ T
cell labeling system to deplete the non-CD4+ cells.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
CB-CD4 Helper T cells can be used to study:
• The role of the T helper cells in intrauterine infections
cytokine release, and effector T cell pathways
• Antigen presentation and antigen-associated activation of
T helper cells for efficient immune response in children
• Classification of T cell subtypes
+
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of 1-2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-CD4+ Helper T Cells
NPB-CD4 + Helper T Cells
Published Papers
Yang, Li, et al. (2008) IL-21 and TGF-Beta are required for differentiation of human TH17 cells. National Institute of Health Public
Access, doi: 10.38/nature07021.
www.allcells.com
For In Vitro Research Use Only
Cord Blood
Purity
Product Description
83
Cord Blood—T Cells—CD8 + Cytotoxic T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-3
5 million/vial
$1299
Frozen
CB020-3F
5 million/vial
$1199
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD8+ Cytotoxic T Cells
Purity
Cord Blood
Product Description
CB-CD8+ Cytotoxic T cells are negatively isolated from mononuclear cells, using an indirect immunomagnetic CD8 labeling
system to deplete the non-CD8 + cells.
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
Application
CB-CD8+ Cytotoxic T cells can be used to study:
• Childhood immunological response to viral infection
• Memory cytotoxic T cell pathways and formation
• Potential cellular engineering to fight cancer cells and
tumors
ALLCELLS
Toll Free Phone: 1-888-535-3444
A minimum of 1-2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
MPB-CD8+ T Cells
NPB-CD8 + T Cells
www.allcells.com
For In Vitro Research Use Only
84
Cord Blood—T Cells—CD4 +/CD45RA+ Naïve T Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB020-4-1
5 million/vial
$999
CB020-4-2
10 million/vial
$1399
CB020-4-3
15 million/vial
$1876
CB020-4-1F
5 million/vial
$799
CB020-4-2F
10 million/vial
$1199
CB020-4-3F
15 million/vial
$1499
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Purity
Product Description
First, CB-CD4+ T Cells are negatively isolated using an
indirect immunomagnetic CD4+ labeling system from
mononuclear cells. Next, CD45RO MicroBeads are used to
deplete the CD45RO+ population, leaving a purified CD4+/
CD45RA + Naïve T Cell population.
Application
CB-CD4+/CD45RA+ Naïve T Cells can be used to study:
• Ex vivo expansion capability of Naive T Cells
• Naive T cell differentiation to memory effector cells
• Comparison of adult CD4+/CD45RA+ Naive T Cells
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity is guaranteed to be at least 85% as assessed by
flow cytometric analysis.
Lead Time
A minimum of 1-2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
Related Products
NPB-CD4+/CD45RA+ T Cells
www.allcells.com
For In Vitro Research Use Only
Cord Blood
CB-CD4+/CD45RA+ Naïve T Cells
85
Cord Blood—CD56 + Natural Killer Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CB023
1 million/vial
$899
Frozen
CB023F
1 million/vial
$799
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CB-CD56+ Natural Killer Cells
Cord Blood
Product Description
Purity
Cord Blood CD56+ Natural Killer (NK) Cells are positively
isolated from MNC using a direct immunomagnetic CD56
MicroBeads labeling system.
The purity is guaranteed to be at least 90% as assessed by
flow cytometric analysis.
Lead Time
Application
A minimum of 1-2 weeks is needed for fresh cells. Product
delivery is subject to stock availability.
CB-CD56+ NK cells can be used to study the following:
• Neonatal infection control
• Anti-leukemia and anti-tumor effect by transplantation
(due to NK cells’ enhanced sensitivity to stimulation and
reduced potential for GVHD)
• Fc receptor binding and ADCC assay for potential use in
therapeutic antibody development
ALLCELLS
Toll Free Phone: 1-888-535-3444
Related Products
MPB-NK Cells
NPB-NK Cells
www.allcells.com
For In Vitro Research Use Only
86
NON-HEMATOPOIETIC STEM CELLS & TISSUE
Non-Hematopoietic Stem Cells Table of Contents
Human Dental Tissue & Mesenchymal Stem Cells
87
Human Dental Tissue & Mesenchymal Stem Cells
Stem cells have been isolated from many tissues and organs, including dental tissue. Five types of dental stem cells have been
established: dental pulp stem cells (DPSC), stem cells from human exfoliated deciduous teeth (SHED), stem cells from apical papilla
(SCAP), periodontal ligament stem cells (PDLSC), and dental follicle stem cells (DFSC). The main characteristics of dental stem cells
are their potential for multilineage differentiation and self-renewal capacity. Dental stem cells can differentiate into odontoblasts,
adipocytes, neuronal-like cells, glial cells, osteoblasts, chondrocytes, melanocytes, myotubes, endothelial cells and pancreatic beta cells.
The multipotency of dental stem cells may be attributable to their embryonic origin which is the neural crest. Similar to bone marrow
mesenchymal cells, DPSCs, SHED, DFSC and PDLSC express CD13, CD44, CD90, CD146 and CD166, CD105 and Stro-1, but are
negative for CD3, CD8, CD11b, CD14, CD15, CD19, CD33, CD34, CD45, and HLA-DR.
For more information visit: www.allcells.com/non-hematopoietic-stem-cells/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
87
Non-Hematopoietic Stem Cells—Dental Pulp Tissue and Stem Cells
Product Format
Cell Type
Catalog #
QTY/Unit
Price/Unit
Follicular Stem Cells
FT002F
1 million/vial
$873
Frozen
Dental Pulp Stem Cells
Adult Teeth
DP003F
1 million/vial
$873
Frozen
Dental Pulp Stem Cells
Deciduous Teeth
DP004F
1 million/vial
$873
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Dental Pulp Tissue—Mesenchymal Stem Cells
Product Description
Non-Hematopoietic: Dental
Stem Cells From Human Exfoliated Deciduous Teeth (SHED) are considered to be the most ‘immature’ of the Dental Stem
Cells and show the highest proliferative capacity when grown in culture. Similar to the DPSCs from third molars, SHED are very
durable in culture and have been passaged over 80 times with no detectable signs of senescence or telomere shortening. SHED
express the embryonic stem cell (ESC) markers Oct-4, Nanog, SSEA-3 and SSEA-4, and tumor recognition antigens (TRA-1-60
and TRA-1-81). When cultured in neurogenic medium, SHED form sphere-like clusters which adhere to the culture dish or float
freely in the culture medium.
Dental Follicle Stem Cells is an ectomesenchymal tissue surrounding the enamel organ and the dental papilla of the developing
tooth germ prior to eruption. The tissue contains the progenitor cells that form the periodontium, (cementum, periodontal ligament,
and alveolar bone). In culture DFSC adhere to plastic, proliferate and show a typical fibroblast-like morphology. DFSCs express
the embryonic marker Oct-4 and the neural progenitor markers Notch-1 and nestin.
Dental Pulp Stem Cells are derived from the pulp tissues of exfoliated deciduous teeth, primary incisors and permanent third molar
teeth. The dental pulp, third molars in particular are a significant stem cell source due to greater numbers of stem cells residing in
the pulp. DPSCs from third molars express developmentally important transcription factors, such as Oct-4, Sox-2, Nanog and c-Myc,
which is an indicator of their pluripotency. Flow-cytometric analysis revealed DPSCs is positive for CD73, CD90, CD105 and CD166,
but negative for CD34, CD45 and CD133, suggesting that these cells are mesenchymal-like stem cells. Under specific culture conditions, DPSCs differentiate into osteogenic, adipogenic and neurogenic cells, as well as form tube-like structures in Matrigel assay.
Application
•
•
Good candidates for future research as a new, powerful tool for therapy.
The efforts made in the research of dental stem cells have clarified many mechanisms underlying the biological processes in
which these cells are involved.
Lead Time
1-2 business days for frozen products. Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
88
DISEASED CELLS & TISSUE
This image was originally published in ASH Image Bank.
Peter Maslak. Primary induction failure: AML - 1. ASH Image Bank. 2011; 2011-1298. The American
Society of Hematology.
This image was originally published in ASH Image Bank.
Gridhar U Adiga; Peter C Cote'; Cheryl L Gardner. Bone marrow necrosis in myelodysplastic syndrome - 5. ASH Image Bank. 2011; 2011-1250. The American Society of Hematology.
ALLCELLS
Toll Free Phone: 1-888-535-3444
This image was originally published in ASH Image Bank.
Ruth Rose. Granular ALL - 1. ASH Image Bank. 2011; 2011-2723. The American Society of
Hematology.
This image was originally published in ASH Image Bank.
John Lazarchick. Leukemic phase of multiple myeloma - 1. ASH Image Bank. 2011; 2011-4094.
The American Society of Hematology.
www.allcells.com
For In Vitro Research Use Only
89
DISEASED CELLS & TISSUE
Diseased Cells & Tissue Table of Contents
Diseased Custom Products
90
Hematological Oncology91-107
Autoimmune108-120
Diseased Cells & Tissue
Resulting from numerous customer requests, AllCells has established a nationwide network of clinical collection sites to
supply various types of disease cells for research use. These facilities supply clinically-relevant, peripheral blood and bone
marrow with sample medical history for use in pre-clinical studies. We have amassed a cell repository of patient samples for
your research needs.
All diseased cells and tissue products are obtained from volunteers participating in an Institutional Review Board (IRB) or
Human Subject Committee approved donor program. Donors sign an informed consent from and are aware of the fact that
these samples are used for research purposes only. Donors must be over the age of 18.
For collections and projects visit us on the web at www.allcells.com/technical-support/ or contact us at sales@allcells.com.
Clinical Information
A Clinical Report will be available for each diseased product. The report may include patient information such as age,
gender, race, weight, height; classification of the disease and staging; and treatment history.
The Clinical Report is a medical information of the patient and not all tests are performed at the time of the blood sample
draw. Please note that all test results are not always concurrent or reflective of each other. Amount of data given per sample
may vary.
Certificate of Analysis
A Certificate of Analysis is provided with the product information; this document details the cell number and the cell viability
of the product vial. All diseased fresh tissue & cell products do not include viral test results as a standard release criteria
prior to delivery. Frozen products are negative for Hepatitis B, Hepatitis C and HIV I/II using antibody based testing.
For more information visit: www.allcells.com/diseased/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
90
DISEASED CUSTOM PRODUCTS
Custom Disease Products & Services
In addition to standard disease catalog products, AllCells offers customized diseased tissue procurement services, and
related cell processing & analysis according to your research project specifications. AllCells collaborates with an
increasing number of IRB-approved clinical collection sites, specialized medical centers and healthcare groups to recruit
the most qualified patient specimens sourced within the Unites States. Examples of bespoke diseased tissue/cell
collection services may include (but are not limited to):
•
Whole blood, bone marrow, serum, plasma, MNCs, purified hematologic cells, urine, and skin lesion biopsy
•
Whole blood from patients with various types of hematologic cancers, solid tumor cancers, infectious, autoimmune,
and other disease types
•
Collection volumes ranging from 10ml to 400ml depending on tissue type and disease type
•
Special collection conditions such as type of anticoagulant e.g. EDTA, sodium heparin, sodium citrate, SST, PST, and
other specialized tubes may be available upon request
•
Patient’s current medical diagnostic stage, treatment stage, drug treatment, lab tests and clinical report
•
Control normal donor samples matched with age, gender, ethnicity; we are able to provide matching tissue and/or cell
types from the same donor
Most fresh tissue specimens can be delivered to USA destinations via FedEx over night and arrive within 24hrs from
collection time. Note: All fresh samples do not include viral test results as standard release criteria prior to delivery
unless otherwise specified. Frozen products include viral tests that are negative for Hep B, Hep C and HIV 1 & 2 . Frozen
products can be shipped worldwide, and are available directly from AllCells or through one of our authorized Distributors.
AllCells Tissue Procurement Team specializes in servicing your specific tissue/cell research project needs. Please contact
your local Sales Representative and/or email us at sales@allcells.com to discuss your project criteria and learn more
about our capabilities in this area.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
91
HEMATOLOGICAL ONCOLOGY CELLS &
TISSUE
Hematological Oncology Table of Contents
Leukemia
Background91-92
Acute Lymphoid Leukemia (ALL)
93-94
ALL Peripheral Blood
93
(Plasma, Mononuclear Cells, CD19+ B
Cells)
ALL Bone Marrow
94
(Plasma, Mononuclear Cells, CD19+ B
Cells)
Acute Myeloid Leukemia (AML)
95-106
AML Peripheral Blood
95
(Plasma, Mononuclear Cells)
AML Bone Marrow
96
(Plasma, Mononuclear Cells, CD34+
Cells)
Chronic Lymphoid Leukemia (CLL)
97-98
CLL Peripheral Blood
97
(Plasma, Mononuclear Cells,
CD19+/CD5+ B Cells)
CLL Bone Marrow
(Plasma, Mononuclear Cells,
CD19+/CD5+ B Cells)
Chronic Myeloid Leukemia (CML)
CML Peripheral Blood
(Plasma, Mononuclear Cells)
CML Bone Marrow
(Plasma, Mononuclear Cells,
CD34+ Cells)
Multiple Myeloma
MM Peripheral Blood
(Plasma, Mononuclear Cells)
MM Bone Marrow
(Plasma, Mononuclear Cells)
Myelodysplastic Syndrome (MDS)
(Mononuclear Cells)
Non-Hodgkin's Lymphoma
98
99-100
99
100
101-103
102
103
104-105
106-107
Hematological Oncology Cells & Tissue
AllCells specializes in highly purified mononuclear cells from peripheral blood and bone marrow. We also provide
specifically selected leukemia blood cells from patients. These samples are made available from newly diagnosed patients
and can be provided in frozen, fresh formats depending on availability. Please contact us for any other special requests.
Background
Leukemia refers to a cancer of the blood-forming (hematopoietic) tissues found in the body. This cancer starts in the bone
marrow and usually spreads quickly into the blood. Over time, leukemia can spread to the lymph nodes, the spleen, the liver,
the covering of the brain and spinal cord, the spinal fluid, and other organs.
There are two ways to classify leukemia: acute or chronic and lymphoid or myeloid. In acute leukemia, the leukemia cells
are derived from early progenitor cells, the immature “blasts.” In chronic leukemia, the leukemia cells are derived from
mature lineage-specific, abnormal cells. These cells live longer than normal cells and build up over time, causing tumors.
Lymphoid leukemia develops from lymphoblasts or lymphocytes in the bone marrow. Myeloid leukemia develops from
myeloid cells in the bone marrow. Consequently, there are four major types of leukemia: acute lymphoid leukemia, acute
myeloid leukemia, chronic lymphoid leukemia, and chronic myeloid leukemia.
Please note that due to the nature of the disease and sensitivity of the cancer patients, product availability is limited.
For more information visit: www.allcells.com/leukemia/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
92
Leukemia—Types—Background
Acute Lymphoid Leukemia (ALL)
Acute lymphoblastic leukemia (ALL), is a form of leukemia,
or cancer of the white blood cells, characterized by excess
lymphoblasts. Malignant, immature white blood cells
continuously multiply and are overproduced in the bone
marrow. ALL causes damage and death by crowding
out normal cells in the bone marrow and by spreading
(metastasizing) to other organs.
Acute Myeloid Leukemia (AML)
Acute myeloid leukemia (AML), also known as acute
myelogenous leukemia, is a cancer of the myeloid line of
blood cells, characterized by the rapid growth of abnormal
white blood cells that accumulate in the bone marrow and
interfere with the production of normal blood cells. AML is
the most common acute leukemia affecting adults, and its
incidence increases with age. As an acute leukemia, AML
progresses rapidly and is typically fatal within weeks or
months if left untreated.
Chronic Myeloid Leukemia (CML)
Chronic myelogenous (or myeloid) leukemia (CML), also
known as chronic granulocytic leukemia (CGL), is a form of
leukemia characterized by the increased and unregulated
growth of predominantly myeloid cells in the bone marrow
and the accumulation of these cells in the blood. CML is a
clonal bone marrow stem cell disorder, in which proliferation
of mature granulocytes (neutrophils, eosinophils, and
basophils) and their precursors is the main finding. CML was
the first malignancy to be linked to a clear genetic abnormality
— the chromosomal translocation known as the
Philadelphia Chromosome.
CML
Chronic Lymphoid Leukemia (CLL)
ALLCELLS
Toll Free Phone: 1-888-535-3444
This image was originally published in ASH Image Bank.
Peter Maslak. Relapsed CML - 1. Bone Marrow aspirate from a patient with increasing WBC
count on imanitib. ASH Image Bank. 2011; 2011-1300. The American Society of Hematology.
Leukemia Types
Chronic Lymphoid Leukemia (CLL) also known as B cell
chronic lymphocytic leukemia (B-CLL) is a slow growing
cancer of the blood that affects older adults. Chronic
Lymphoid Leukemia cells originate in the bone marrow and
then spread to the blood and lymph nodes. Characterization
by flow cytometry demonstrates that these CLL cells are
CD19+ and CD5+. Prognosis of CLL is dependent on the
cytogenic profile of the cancer. Deletions of part of the short
arm of chromosome 17 (del 17p) or deletions of the long arm
on chromosome 11 (del 11q) indicate a poorer outcome while
deletions of the long arm of chromosome 13 (del 13q) favors
a better prognosis for CLL patients.
www.allcells.com
For In Vitro Research Use Only
93
Acute Lymphoid Leukemia
Diseased Cells—ALL—Peripheral Blood—Plasma, Mononuclear Cells, CD19 +
B Cells
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-PB011F
5 ml
$207
10 million/vial
$687
ALL-B006F
5 million/vial
$2590
ALL-B007F
10 million/vial
$4114
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
ALL-PB001F
Newly Diagnosed/Untreated CD19+B Cells
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-PB-Plasma
Product Description
Purity
Leukemia: ALL
ALL-PB-Plasma is obtained by centrifugation of ALL peripheral
blood. This product has a diminished number of cells. ALL-Plasma
are available in the newly diagnosed/untreated stages.
ALL-PB-MNC are isolated from ALL peripheral blood by diluting the
whole blood with PBS and using gradient separation techniques.
After centrifugation, the mononuclear cell layer is collected. MNC
can be processed further to isolate subpopulations. ALL-MNC are
available in the newly diagnosed/untreated stage.
ALL-PB-CD19+ B cells are positively isolated from adult ALL
peripheral blood MNC, using a direct immunomagnetic CD19
MicroBead labeling system. ALL-PB-CD19+ B cells are available in
the newly diagnosed/untreated stages.
Application
ALL-PB-Plasma can be used to study ALL lymphoblast and
lymphocyte secretion in the plasma.
ALL-PB-MNC can be used research new drug candidates, including
various molecular and genetic drug delivery methods. Study any
negative effects of treatment on non-malignant cells and to help
minimize damage to non-leukemia cells.
ALL-CD19+ B Cells can be used for cytogenetic and mutational
analysis of B-ALL diseases, such as ALL-L3 or Burkitt’s
lymphoma/leukemia.
ALLCELLS
Toll Free Phone: 1-888-535-3444
The purity of CD19+ B Cells is guaranteed to be at least 90% as
assessed by flow cytometric analysis.
Lead Time
Product delivery is subject to stock availability.
Related Products
ALL-BM-MNC
NPB-Plasma
NPB-MNC
ALL-BM-CD19+ B Cells
NPB-BM-CD19 + B Cells
www.allcells.com
For In Vitro Research Use Only
94
Diseased Cells—ALL—Bone Marrow—Plasma, Mononuclear Cells, CD19 +B
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
ALL-BM011F
5 ml
$207
10 million/vial
$687
ALL-B012F
5 million/vial
$1374
ALL-B013F
10 million/vial
$2200
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
ALL-BM001F
Newly Diagnosed/Untreated CD19+ B Cells
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
ALL-BM-Plasma
Product Description
Purity
ALL-BM plasma is obtained by centrifugation of the ALL-bone
marrow. This product has a diminished number of cells and platelets.
ALL-BM-MNC are isolated from bone marrow by diluting the
whole bone marrow with phosphate-buffered saline and using
gradient separation techniques. MNC can be processed to isolate
subpopulations.
ALL-BM-CD19+ B cells are positively isolated from adult ALL-BMMNC, using a direct immunomagnetic CD19 MicroBead labeling
system.
The purity of CD19+ B Cells is guaranteed to be at least 90% as
assessed by flow cytometric analysis.
Lead Time
Product delivery is subject to stock availability.
Application
Leukemia: ALL
Related Products
ALL-PB-Plasma
ALL-PB-MNC
ALL-PB-CD19+
ALL-BM-Plasma can be used to research B- lymphoblast secretion
and activity in bone marrow.
ALL-BM-MNC can be used as a possible source for isolating
leukemia stem cells.
ALL-BM-CD19+ can be used to study leukemia stem/progenitor
cells for precursor B acute lymphoblastic leukemia/lymphoma,
research molecular mechanisms for B cell loss of function and
malignancy and serve as diagnostic tools to aid classification of
various subtypes of B-ALL
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
95
Acute Myeloid Leukemia
Diseased Cells—AML—Peripheral Blood—Plasma, Mononuclear Cells
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-PB011F
5 ml
$219
10 million/vial
$713
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
AML-PB001F
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Lead Time
AML-PB-Plasma is obtained by centrifugation of AML peripheral
blood. AML-PB-plasma has a diminished number of cells and
platelets. AML plasma is available in the newly diagnosed/untreated
stages.
AML-PB-MNC are isolated from AML-PB by diluting the blood
with phosphate-buffered saline and using gradient separation
techniques. After centrifugation, the MNC layer is collected. MNC
can be processed further to isolate subpopulations.
Product delivery is subject to stock availability.
Related Products
AML-BM-Plasma
NPB-Plasma
Application
Leukemia: AML
AML-PB-Plasma can be used to study and analyze leukemia cell
and progenitor cell protein secretion into the serum or plasma.
AML-PB-MNC can be used to test the efficacy of different
diagnostic tools (e.g. cellular morphology, immunocytometry,
and cytogenetics).
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
96
Diseased Cells—AML—Bone Marrow—Plasma, Mononuclear Cells,
CD34 +Cells
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
AML-BM015F
5 ml
$219
10 million/vial
$728
AML-BM006F
1 million/vial
$1488
AML-BM007F
5 million/vial
$6085
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
AML-BM001F
Newly Diagnosed/Untreated CD34+ Cells
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Purity
AML-BM Plasma is obtained by centrifugation of the adult
AML-BM. Plasma has a diminished number of cells
and platelets.
BM is obtained from adult AML patients. BM-MNC are
isolated by diluting the bone marrow with phosphatebuffered saline and using gradient separation techniques.
After centrifugation, the MNC layer is collected. MNC can be
processed further to isolate cell subpopulations.
AML-BM-CD34+ cells are selected from adult AML-BMMNC. The BM-CD34+ stem/progenitor cells are positively
isolated using a direct immunomagnetic CD34 MicroBead
labeling system.
The purity is guaranteed to be at least 90% as assessed by flow
cytometric analysis.
Lead Time
Product delivery is subject to stock availability.
Related Products
AML-PB-Plasma
BM-Plasma
BM-CD34+ Cells
AML-PB-CD34+ Cells
Leukemia: AML
Application
AML-BM Plasma can be used to study and analyze possible
leukemia stem and progenitor cell protein secretion into the
serum plasma.
AML-BM-MNC can be used to study the induction
chemotherapy effects performing as general cell
suppressors of active proliferation.
AML-BM-CD34+ cells can be used to research novel genetic
mutations, such as in Flt-3, for subtyping and
therapeutic research.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
97
Chronic Lymphoid Leukemia
Diseased Cells—CLL—Peripheral Blood—Plasma, Mononuclear Cells,
CD19 +/CD5 + B Cells
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-PB011F
5 ml
$213
CLL-PB001F
10 million/vial
$708
CLL-PB002F
50 million/vial
$1697
CLL-B013F
1 million/vial
$637
CLL-B014F
5 million/vial
$1654
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
Newly Diagnosed/Untreated CD19+/CD5+
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Purity
Product Description
Leukemia: CLL
CLL-PB Plasma is obtained by centrifugation of the CLL-PB-MNC.
Plasma has a diminished number of cells and platelets.
CLL-PB-MNC are isolated from CLL-peripheral blood by diluting the
blood with phosphate-buffered saline and using gradient separation.
MNC can be processed to isolate subpopulations.
CLL-PB-CD19+/CD5+ B cells are isolated from CLL peripheral
blood mononuclear cells using a direct immunomagnetic CD19+
MicroBead labeling system.
The purity of CD19+/CD5+s guaranteed to be at least 90% as
assessed by flow cytometric analysis.
Lead Time
Product delivery is subject to stock availability.
Application
CLL-PB Plasma can be used to study the cell secretion and protein
analysis into the serum or plasma.
CLL-PB-MNC can be used to research improved methods for
diagnosis and prognosis from early to late CLL stages as well as
study B lymphocytosis effects on other cells, which cause problems,
such
as cytoreduction.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
98
Diseased Cells—CLL—Bone Marrow—Plasma, Mononuclear Cells,CD19 +/
CD5 + B Cells
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CLL-BM011F
5 ml
$213
10 million/vial
$708
CLL-B037F
1 million/vial
$708
CLL-B038F
5 million/vial
$1978
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
CLL-BM001F
Newly Diagnosed/Untreated CD19+/CD5+ B Cells
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Purity
Product Description
CLL BM-Plasma is obtained by centrifugation of the CLL bone
marrow. Plasma has a diminished number of cells and platelets.
CLL-BM-MNC are isolated by diluting CML bone marrow
with phosphate-buffered saline and using gradient separation
techniques. After centrifugation, the MNC layer is collected. CLLBM-MNC can be processed further to isolate subpopulations.
CLL-PB-CD19+/CD5+ B cells are isolated from CLL bone
marrow mononuclear cells using a direct immunomagnetic CD19+
MicroBead labeling system.
The purity of CLL-PB-CD19+/CD5+ is guaranteed to be at least
90% as assessed by flow cytometric analysis.
Related Products
CLL-PB-Plasma
BM-Plasma
Application
ALLCELLS
Toll Free Phone: 1-888-535-3444
Leukemia: CLL
CLL-BM-Plasma can be used to study extracellular proteins that
could play a role in the cause and progression of the CLL-B
cell malignancies.
CLL-BM-MNC can be used to examine the capacity to generate
cellular immune response to newly established CLL-associated
antigens.
CLL-PB-CD19+/CD5+ B cells can be used to study the transition
from the mantle zone to the bone marrow microenvironment.
www.allcells.com
For In Vitro Research Use Only
99
Chronic Myeloid Leukemia
Diseased Cells—CML—Peripheral Blood—Plasma, Mononuclear Cells
Chronic Phase Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-PB011F
5 ml
$210
CML-PB001F
10 million/vial
$2792
Chronic Phase Mononuclear Cells
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CML-PB-Plasma
Product Description
Lead Time
CML-PB Plasma is obtained by centrifuging CML peripheral blood.
Plasma has a diminished number of cells and platelets. CML
plasma is available in the chronic phase and accelerated or blast
crisis phase.
CLL-PB-MNC are isolated from CML peripheral blood by diluting
the blood with phosphate-buffered saline and using gradient
separation techniques. After centrifugation, the MNC layer is
collected. MNC can be processed further to isolate subpopulations.
Product delivery is subject to stock availability.
Related Products
CML-BM-Plasma
CML-BM-MNC
NPB-MNC
Leukemia: CML
Application
CML-PB Plasma can be used to study Leukemia cell secretion in
to the serum or plasma and other possible leukemia candidate and
target proteins and pathways in the serum or plasma
CML-PB-MNC can be used to improve methods of diagnosis,
classification, and prognosis. In addition, researchers can use these
cells to study leukemia cells’ ability to affect and expel healthy cells.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
100
Diseased Cells—CML—Bone Marrow—Plasma, Mononuclear Cells,
CD34 +Cells
Chronic Phase Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
CML-BM011F
5 ml
$211
CML-BM001F
10 million/vial
$2959
CML-BM008-1F
0.2 million/vial
$1544
CML-BM008-2F
1 million/vial
$5229
Chronic Phase Mononuclear Cells
Frozen
Chronic Phase CD34+
Frozen
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
CML-PB-MNC
Background
Application
Chronic Myeloid Leukemia (CML) is often divided into three
phases, based on clinical characteristics and laboratory findings. In
the absence of interventions, CML typically begins in the chronic
phase (85% of CML patients). Over the course of several years, the
cancer progresses to an accelerated phase and ultimately to a blast
crisis phase.
Product Description
ALLCELLS
Accelerated/Blast Crisis - CML-PB-MNC can be used to
improve methods of diagnosis, classification, and prognosis.
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Related Products
CML-BM-MNC
NPB-MNC
www.allcells.com
For In Vitro Research Use Only
Leukemia: CML
Lead Time
CLL-PB-MNC are isolated from CML peripheral blood by diluting
the blood with phosphate-buffered saline and using gradient
separation techniques. After centrifugation, the MNC layer is
collected. MNC can be processed further to isolate subpopulations.
CML-PB-MNC are available in the chronic phase and accelerated
or blast crisis phase.
Chronic Phase - CML-PB-MNC can be used to improve methods of
diagnosis, classification, and prognosis. In addition, researchers can
use these cells to study leukemia cells’ ability to affect and expel
healthy cells.
101
MULTIPLE MYELOMA
Multiple Myeloma (MM) Table of Contents
MM Peripheral Blood
MM Bone Marrow
(Plasma, Mononuclear Cells)
102
(Plasma, Mononuclear Cells)
103
Multiple Myeloma Cells & Tissue
Background
Multiple Myeloma (MM) is a type of incurable cancer of the plasma cells. Normal plasma cells make antibodies that attack
foreign microorganisms and viruses in the body. MM occurs when these plasma cells grow out of control, becoming
malignant and form a tumor in the bone marrow. MM has several types of characterizations. An excessive amount of
plasma cells can cause problems in the bone marrow where blood cells are made. Consequently, the bone marrow may not
be able to make enough red blood cells, platelets, or normal white blood cells to sustain normal body functions.
Please note that due to the nature of the disease and sensitivity of the cancer patients, product availability is limited.
For more information visit: www.allcells.com/multiple-myeloma/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
102
Diseased Cells—MM—Peripheral Blood—Plasma, Mononuclear Cells
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MM-PB011F
5 ml
$277
10 million/vial
$1160
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
MM-PB001F
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Lead Time
Product Description
MM-PB-Plasma is obtained by centrifugation of MM peripheral
blood. Plasma has a diminished number of cells.
MM-PB-MNC are isolated from MM-peripheral blood by diluting the
blood with phosphate-buffered saline and using gradient separation
techniques. After centrifugation, the MNC layer is collected. MNC
can be processed further to isolate subpopulations.
Application
MM-PB-Plasma can be used to study production and manifestation
of free serum or urine immunoglobulin, or paraprotein. These
paraproteins are indicators of disease stages.
Product delivery is subject to stock availability.
Related Products
BM-MNC
MM-BM-MNC
Publications
Andrew T. Hutchinson et al. (2013) Formation of Assemblies on
Cell Membranes by Secreted Proteins: Molecular Studies of Free
Lambda Light Chain Aggregates Found on the Surface of Myeloma..
Biochemical Journal Immediate Publication http://www.allcells.com/
publications/0186allcells.pdf
Multiple Myeloma
MM-PB-MNC can be used to find more efficient ways of
diagnosing effects from specific translocations, such as FGFR3
over expression.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
103
Diseased Cells—MM—Bone Marrow—Plasma, Mononuclear Cells
Newly Diagnosed/Untreated Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MM-BM011F
5 ml
$277
10 million/vial
$1599
Newly Diagnosed/Untreated Mononuclear Cells
Frozen
MM-BM001F
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Lead Time
MM-BM-Plasma
MM-BM Plasma is obtained by centrifuging the MM-bone marrow.
The plasma has a diminished number of cells and platelets.
MM-BM-MNC are isolated by diluting MM-bone marrow with
phosphate-buffered saline and using gradient separation.
Application
Related Products
MM-PB-Plasma
NPB-Plasma
BM-MNC
MM-PB-MNC
Multiple Myeloma
MM-BM-Plasma can be used to study bone deterioration and lesions
(by detecting of hypercalcemia) and hyperviscosity
syndrome therapies.
MM-PB-Plasma can be used to study production and manifestation
of free serum or urine immunoglobulin, or paraprotein. These
paraproteins are indicators of disease stages.
Product delivery is subject to stock availability.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
104
MYELODYSPLASTIC SYNDROME (MDS)
Myelodysplastic Syndrome (MDS) Table of Contents
MDS Bone Marrow Mononuclear Cells
105
Myelodysplastic Syndrome Cells & Tissue
Background
The myelodysplastic syndromes (MDS, formerly known as “preleukemia”) are a diverse collection of hematological conditions united
by ineffective production (dysplasia) of myeloid blood cells; these conditions risk transformation to acute myelogenous leukemia
(AML). Myelodysplastic syndromes are bone marrow stem cell disorders resulting in disorderly and ineffective hematopoiesis
manifested by irreversible quantitative and qualitative defects in hematopoietic cells. In a majority of cases, the course of disease is
chronic with gradually worsening cytopenias due to progressive bone marrow failure. Approximately one-third of patients with MDS
progress to AML within months to a few years.
Please note that due to the nature of the disease and sensitivity of the cancer patients, product availability is limited.
For more information visit: www.allcells.com/multiple-myeloma/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
105
Diseased Cells—Myelodysplastic Syndrome (MDS)—Bone Marrow—
Mononuclear Cells
Newly Diagnosed/Untreated Mononuclear Cells
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen
MDS-BM001F
10 million/vial
$1023
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
MDS-BM-MNC
Lead Time
Product Description
AllCells has isolated mononuclear cells (MNCs) from the bone
marrow of Myelodysplastic Syndrome (MDS) patients. Patients
participating in our MDS donor program have been characterized as
Newly Diagnosed/Untreated.
Related Products
AML-BM-MNC
Myelodysplastic Syndrome
Frozen MDS BM MNC - BM is obtained from adult Myelodysplastic
Syndrome patients. BM-MNC are isolated by diluting the bone
marrow with phosphate-buffered saline and using gradient
separation techniques. After centrifugation,the MNC layer
is collected. MNC can be processed further to isolate cell
subpopulations.
Product delivery is subject to stock availability.
This image was originally published in ASH Image Bank.
Peter Maslak. Post-BMT aspirate - 1. ASH Image Bank. 2011; 2011-3540. The American
Society of Hematology.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
106
NON-HODGKIN'S LYMPHOMA
Non-Hodgkin's Lymphoma Cells & Tissue
AllCells is able to provide custom collections of B-cell lymphomas, including Diffuse Large B-cell Lymphoma (DLBCL),
Mantle Cell Lymphoma (MCL), Follicular Lymphoma (FLL), and Waldenstrom's Macroglobulinemia (WM) from either bone
marrow and/or peripheral blood samples of newly diagnosed/untreated or relapsed/refractory patients. Medical history
of each patient is available and may include treatment history, serum biochemistry, bone marrow aspirate smear analysis,
cytogenics, and immunophenotyping.
Background
Lymphomas are types of cancers derived from lymphocytes and are typically treated by combinations of chemotherapy,
monoclonal antibodies, immunotherapy, radiation, and hematopoietic stem cell transplantation.
Non-Hodgkin’s Lymphoma (NHL) is a collective term for a broad range of lymphomas that are not classified as
Hodgkin’s lymphoma. NHL can either be a cancer of B cells or T cells. B-cell non-Hodgkin lymphomas include
Burkitt’s lymphoma, diffuse large B-cell lymphoma, follicular lymphoma, immunoblastic large cell lymphoma, precursor
B-lymphoblastic lymphoma, and mantle cell lymphoma. T-cell non-Hodgkin lymphomas include anaplastic large cell
lymphoma, and precursor T-lymphoblastic lymphoma.
For custom collections and projects visit us on the web at www.allcells.com/technical-support/ or
contact us at sales@allcells.com.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
107
Diseased Cells-Non-Hodgkin's Lymphoma
DLBCL
Follicular Lymphoma (FLL)
Follicular Lymphoma (FLL) is a type of “indolent” NonHodgkin’s Lymphoma and is defined by follicule center B
Cells, which express a follicular pattern. FLL’s genetic origin
has been correlated to t(14;18), which causes excessive
expression of the Bcl-2 gene, and some reported cases Bcl6 translocation at 3q27. Typically, follicular B cells express
surface-markers CD10, but not CD5. The “tumors” or lymph
follicles contain a mixture of “small” cleaved follicle center
cells (centrocytes) and “large” non-cleaved follicle center
cells (centroblasts): the former usually in predominance. FLL
is generally indolent but can sometimes progress to more
aggressive large cell lymphomas, such as DLBCL.
This image was originally published in ASH Image Bank.
John Lazarchick. Lymphomatous peritoneal effusion - 3. Mature B-cell neoplasms. ASH Image
Bank. 2011; 2011-3729. The American Society of Hematology.
Diffuse Large B Cell Lymphoma (DLBCL)
Diffuse Large B Cell Lymphoma (DLBCL) is an “intermediategrade” and aggressive type of malignant B cell lymphoma.
DLBCL can arise from an immunoblastic origin, or can
transform from the follicule center large cell neoplasm.
This transformed cell can be further classified into large
non cleaved (centroblastic) cells or large cleaved (large
centrocytic) cells. DLBCL is frequently associated with
t(8;14) and t(14;18): chromosomal rearrangements pertaining
to Myc and Bcl-2 genes. In addition, there may be some
chromosomal mutation involving Bcl-6 on chromosome 3.
Waldenström's Macroglobulinemia (WM)
Waldenström’s Macroglobulinemia (WM), also known as
lymphoplasmacytic lymphoma, WM is one of the rarest types
of NHL. WM is a malignant small lymphocytic lymphoma
with plasmacytoid features. While this disease has not been
linked to a single mutation, researchers have found a WM
associated with the 6p21.3 locus. WM is characterized by
large monoclonal IgM in the serum and symptoms, such as
peripheral neuropathy and hyperviscosity syndrome. WM is
distinguished from Multiple Myeloma by its indolent nature and
less frequent bone marrow involvement. Patient monitoring is
usually recommended before any necessary treatment.
DLBCL can also be called “histiocytic” lymphoma, which
refers to the morphologic expression of the transformed
lymphocytes. DLBCL is the most frequent type of extranodal
lymphoma with the least common bone marrow involvement.
Mantle Cell Lymphoma (MCL)
Mantle Cell Lymphoma (MCL) is one of the rarer types of NHL.
MCL is a disease of the naïve pre-germinal center B cells,
located in the “mantle zone” surrounding the normal germinal
center follicles. A distinctive translocation at t(11;14)(q13;q32)
causes upregulation of Bcl-1, development arrest, and unlimited
proliferation of the mantle-zone B cells. This chromosomal
translocation causes the over-expression of Bcl-1’s protein
product, Cyclin-D1. In addition, these mantle-zone B cells
express the CD5 marker. MCL is one of the few NHLs that can
cross the blood-brain barrier.
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
108
AUTOIMMUNE CELLS & TISSUE
Autoimmune Table of Contents
Graves' Thyroid
109
Rheumatoid Arthritis (RA)
114
Hashimoto's thyroiditis
110
Systemic Lupus Erythematosus
115
Multiple Sclerosis (MS)
111
Type I Diabetes (DIA1)
116
Type II Diabetes (DIA2)
117
Osteoarthritis (OA)112
Psoriasis (PSO)113
Autoimmune Cells & Tissue
AllCells provides patient samples of Autoimmune cells isolated from Rheumatoid Arthritis, , Osteoarthritis, Systemic Lupus
Erythematosus (SLE), Psoriasis, Type I & Type II Diabetic, and Multiple Sclerosis patients. Autoimmune plasma and autoimmune
peripheral blood mononuclear cells are available from each disease type. These are available in frozen or fresh formats upon
request.
Background
Autoimmune Diseases arise from an inappropriate immune response of the body against substances and tissues normally present
within the body. This disease may be restricted to certain organs or involve a particular tissue located in the body. Treatment of
Autoimmune Diseases is typically with immunosuppression or medication which decreases the
immune response.
For a disease to be classified as an Autoimmune Disease there must be direct evidence from transfer of pathogenic antibodies or
pathogenic T cells, indirect evidence based on reproduction of the autoimmune disease in experimental animals, or circumstantial
evidence from clinical clues.
Autoimmune specimens can be used in preclinical research, drug discovery, drug toxicity, biomarker development, and pre-selection
of indication for clinical trials.
For more information visit: www.allcells.com/autoimmune/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
109
Diseased Cells—Graves' Thyroid (GTR)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
GTR-WB40-VK2
40 ml
$1418
GTR-WB100-VK2
100 ml
$1838
GTR-WB400-VK2
400 ml
$3203
GTR-WB400-VNH
400 ml
$3203
GTR-WB011F-VK2
5 ml
$215
GTR-WB011F-VNH
5 ml
$215
GTR-WB001F
10 million/vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Autoimmune: Graves' Thyroid
Product Description
Lead Time
Fresh OA GTR -Whole blood is drawn from Graves' Thyroid
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen GTR PB Plasma - Plasma is isolated from peripheral blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. Graves' Thyroid peripheral blood plasma is obtained by
centrifugation of the Graves' Thyroid PB.
Frozen GTR PB MNC - Peripheral blood mononuclear cells are
isolated from Graves' Thyroid peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Graves' Thyroid
PB MNC is available upon request.
Product delivery is subject to stock availability.
Background
Graves' disease is an immune system disorder that results in the
overproduction of thyroid hormones (hyperthyroidism). It is more
common in women than in men.
Treatment generally involves inhibiting the overproduction of
thyroid hormones.
Application
Graves' Thyroid specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
110
Diseased Cells—Hashimoto's Thyroiditis (HTR)—WholeBlood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
HTR-WB40-VK2
40 ml
$1418
HTR-WB100-VK2
100 ml
$1838
HTR-WB400-VK2
400 ml
$3203
HTR-WB400-VNH
400 ml
$3203
HTR-WB011F-VK2
5 ml
$215
HTR-WB011F-VNH
5 ml
$215
HTR-WB001F
10 million/vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Lead Time
Product delivery is subject to stock availability.
Background
Hashimoto's disease is a condition in which your immune system
attacks your thyroid. Inflammation from Hashimoto's disease,
also known as chronic lymphocytic thyroiditis, often leads to an
underactive thyroid gland.
Treatment may includes hormone replacement therapy.
Application
HTR specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
Autoimmune: HTR
Fresh HTR WB- Whole blood is drawn from Hashimoto's Thyroiditis
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen HTR PB Plasma - Plasma is isolated from peripheral blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. HTR peripheral blood plasma is obtained by centrifugation
of the HTR PB.
Frozen HTR PB MNC - Peripheral blood mononuclear cells are
isolated from HTR peripheral blood by diluting the whole blood with
PBS and using gradient separation techniques. After centrifugation,
the mononuclear cell layer is collected. MNC can be processed
further to isolate subpopulations. Fresh HTR PB MNC is available
upon request.
111
Diseased Cells—Multiple Sclerosis (MS)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
MS-WB40-VK2
40 ml
$1418
MS-WB100-VK2
100 ml
$1838
MS-WB400-VK2
400 ml
$3203
MS-WB400-VNH
400 ml
$3203
MS-PB011F-VK2
5 ml
$215
MS-PB011F-VNH
5 ml
$215
MS-PB001F
10 million/ vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Autoimmune: Multiple Sclerosis
Product Description
Lead Time
Fresh MS WB- Whole blood is drawn from Multiple Sclerosis
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen MS PB Plasma - Plasma is isolated from pheriperal blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. MS peripheral blood plasma is obtained by centrifugation of
the MS PB.
Frozen MS PB MNC - Peripheral blood mononuclear cells are
isolated from MS peripheral blood by diluting the whole blood with
PBS and using gradient separation techniques. After centrifugation,
the mononuclear cell layer is collected. MNC can be processed
further to isolate subpopulations. Fresh MS PB MNC is available
upon request.
Application
MS specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Background
Multiple Sclerosis also known as (Disseminated Sclerosis or
Encephalomyelitis Disseminata) is an inflammatory disease in which
the fatty myelin sheaths around the axons of the brain and spinal
cord are damaged leading to demyelination and scarring.
The disease usually occurs in young adults and is more common in
women than in men. In MS the body’s own immune system attacks
the myelin. When myelin is lost, the axons can no longer effectively
conduct electrical signals.
Most likely MS occurs as a result of some combination of genetic,
environmental, and infectious factors. Epidemiological studies of MS
have provided hints on possible causes for the disease however the
exact cause of the disease is still unknown.
www.allcells.com
For In Vitro Research Use Only
112
Diseased Cells—Osteoarthritis (OA)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
OA-WB40-VK2
40 ml
$1418
OA-WB100-VK2
100 ml
$1838
OA-WB400-VK2
400 ml
$3203
OA-WB400-VNH
400 ml
$3203
OA-PB011F-VK2
5 ml
$215
OA-PB011F-VNH
5 ml
$215
OA-PB001F
10 million/vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Lead Time
Fresh OA WB -Whole blood is drawn from Osteoarthritis patients in
your choice of anticoagulant: EDTA(VK2) or sodium heparin(VNH).
Frozen OA PB Plasma - Plasma is isolated from peripheral blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. Osteoarthritis peripheral blood plasma is obtained by
centrifugation of the Osteoarthritis PB.
Frozen OA PB MNC - Peripheral blood mononuclear cells are
isolated from Osteoarthritis peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Osteoarthritis PB
MNC is available upon request.
Application
Osteoarthritis specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Background
Osteoarthritis also known as Degenerative Arthritis or Degenerative
Joint Disease, is a group of mechanical abnormalities involving
degradation of joints, including articular cartilage and subchondral
bone. A variety of causes (heredity, developmental, metabolic, and
mechanical) may initiate processes leading to a loss of cartilage.
Treatment generally involves a combination of exercise, lifestyle
modification, and analgesics. OA is the most common form of
arthritis and the leading cause of chronic disability in the United
States, affecting 27 million people. Osteoarthritis is a highly-studied
indication within the therapeutic area of Inflammatory Diseases.
www.allcells.com
For In Vitro Research Use Only
Autoimmune: Osteoarthritis
Product Description
113
Diseased Cells—Psoriasis (PSO)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
PSO-WB40-VK2
40 ml
$1418
PSO-WB100-VK2
100 ml
$1838
PSO-WB400-VK2
400 ml
$3203
PSO-WB400-VNH
400 ml
$3203
PSO-PB011F-VK2
5 ml
$215
PSO-PB011F-VNH
5 ml
$215
PSO-PB001F
10 million/vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Autoimmune: Psoriasis
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Lead Time
Fresh PSO WB - Whole blood is drawn from Psoriasis patients in
your choice of anticoagulant: EDTA(VK2) or sodium heparin(VNH).
Frozen PSO PB Plasma - Plasma is isolated from peripheral
blood collected into your choice of anticoagulant: EDTA(VK2) or
sodium heparin. Psoriasis peripheral blood plasma is obtained by
centrifugation of the Psoriasis PB.
Frozen PSO PB MNC - Peripheral blood mononuclear cells are
isolated from Psoriasis peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Psoriasis PB
MNC is available upon request.
Product delivery is subject to stock availability.
Application
Psoriasis specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Background
Psoriasis is an autoimmune disease that appears on the skin and
occurs when epidermal cells cause an immune response that in turn
speeds up the growth cycle of skin cells. Psoriasis has been linked
to an increased risk of stroke.
The disorder is a chronic recurring condition that varies in severity
from minor localized patches to complete body coverage. Psoriasis
can also cause inflammation of the joints which is known as
psoriatic arthritis. Between 10-30% of all people with psoriasis
experience psoriatic arthritis.
The cause of psoriasis is not fully understood but it is believed to
have a genetic component. Various environmental factors have
been suggested as aggravating to psoriasis including stress
and withdrawal of systemic corticosteroids. Psoriasis has a large
hereditary component involving many genes however it is unclear
how these genes work together. Many involve the immune system,
particularly the major histocompatibility complex (MHC) and T cells.
www.allcells.com
For In Vitro Research Use Only
114
Diseased Cells—Rheumatoid Arthritis (RA)—Whole Blood,Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
RA-WB40-VK2
40 ml
$1418
RA-WB100-VK2
100 ml
$1838
RA-WB400-VK2
400 ml
$3203
RA-WB400-VNH
400 ml
$3203
RA-PB011F-VK2
5 ml
$215
RA-PB011F-VNH
5 ml
$215
RA-PB001F
10 million/vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Lead Time
Fresh RA WB - Whole blood is drawn from Rheumatoid Arthritis
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen RA PB Plasma - Plasma is isolated from peripheral blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. RA peripheral blood plasma is obtained by centrifugation of
the RA PB.
Frozen RA PB MNC - Peripheral blood mononuclear cells are
isolated from Rheumatoid Arthritis peripheral blood by diluting the
whole blood with PBS and using gradient separation techniques.
After centrifugation, the mononuclear cell layer is collected. MNC
can be processed further to isolate subpopulations.
Application
OA specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Background
Rheumatoid Arthritis is a chronic inflammatory disorder affecting
many tissues and organs but principally attacks the synovial
joints. About 1% of the world’s population is afflicted by
rheumatoid arthritis; women 3x more often than men. Onset is
most frequent between the ages of 30-40 but people of any age
can be affected.
When RA is clinically suspected, tests for the presence of
rheumatoid factor (RF) are conducted. A negative RF does not
rule out a positive diagnosis; rather the arthritis is characterized
as seronegative. This is the case in about 15% of patients.
Rheumatoid Arthritis is a highly-studied indication within the
therapeutic area of Inflammatory Diseases.
www.allcells.com
For In Vitro Research Use Only
Autoimmune: Rheumatoid Arthritis
Product Description
115
Diseased Cells—Systemic Lupus Erythematosus (SLE)—Whole Blood,
Plasma, Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
SLE-WB40-VK2
40 ml
$1418
SLE-WB100-VK2
100 ml
$1838
SLE-WB400-VK2
400 ml
$3203
SLE-WB400-VNH
400 ml
$3203
SLE-PB011F-VK2
5 ml
$215
SLE-PB011F-VNH
5 ml
$215
10 million/ vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
SLE-PB001F
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Autoimmune: SLE
Product Description
Lead Time
Fresh SLE WB- Whole blood is drawn from Systemic Lupus
Erythematosus patients in your choice of anticoagulant: EDTA(VK2)
or sodium heparin(VNH).
Frozen SLE PB Plasma - Plasma is isolated from peripheral blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. SLE peripheral blood plasma is obtained by centrifugation
of the SLE PB.
Frozen SLE PB MNC - Peripheral blood mononuclear cells are
isolated from MS peripheral blood by diluting the whole blood with
PBS and using gradient separation techniques. After centrifugation,
the mononuclear cell layer is collected. MNC can be processed
further to isolate subpopulations. Fresh MS PB MNC is available
upon request.
Application
MS specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Background
Systemic Lupus Erythematosus is a Type III Hypersensitivity
Reaction caused by an antibody immune complex formation. Often
referred to as 'lupus', SLE is a systemic autoimmune disease that
can affect any part of the body.
SLE is treatable using immunosuppression, mainly with
cyclophosphamide, corticosteroids, and other immunosuppressants.
There is currently no cure for SLE. SLE can be fatal although with
recent medical advances, fatalities are becoming increasingly rare.
Survival for people with SLE in the United States and Canada has
risen to approximately 95% at 5 years, 90% at 10 years, and 78%
at
20 years.
www.allcells.com
For In Vitro Research Use Only
116
Diseased Cells—Type I Diabetes (DIA1)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
DIA1-WB40-VK2
40 ml
$1418
DIA1-WB100-VK2
100 ml
$1838
DIA1-WB400-VK2
400 ml
$3203
DIA1-WB400-VNH
400 ml
$3203
DIA1-PB011F-VK2
5 ml
$215
DIA1-PB011F-VNH
5 ml
$215
DIA1-PB001F
10 million/vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Lead Time
Fresh DIA1 WB - Whole blood is drawn from Type I Diabetes
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen DIA1 PB Plasma - Plasma is isolated from peripheral blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. Type I Diabetes peripheral blood plasma is obtained by
centrifugation of the Type I Diabetes PB.
Frozen DIA1 PB MNC - Peripheral blood mononuclear cells are
isolated from Type I Diabetes peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation, the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations.
Application
DIA1 specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Background
Diabetes Type I also referred to as Diabetes Mellitus Type I (formally
juvenile diabetes), results from autoimmune destruction of insulinproducing beta cells of the pancreas. The subsequent lack of insulin
leads to an increase of blood and urine glucose.
Eventually Type I Diabetes is fatal unless treated with insulin.
Pancreatic transplants have been used to replenish a natural supply
of insulin. Pancreatic islet cell transplantation is experimental
though more procedures are occurring annually.
Type I Diabetes is a polygenic disease. Depending on locus or
combination of loci, it can be dominant, recessive, or somewhere inbetween. The strongest gene, IDDM1, is located in the MHC Class
II region on chromosome 6 at staining region 6p21. The appearance
of Diabetes-related autoantibodies has been shown to be able to
predict the appearance of Diabetes Type I before any
hyperglycemia occurs.
www.allcells.com
For In Vitro Research Use Only
Autoimmune: DIA1
Product Description
117
Diseased Cells—Type II Diabetes (DIA2)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
DIA2-WB40-VK2
40 ml
$1418
DIA2-WB100-VK2
100 ml
$1838
DIA2-WB400-VK2
400 ml
$3203
DIA2-WB400-VNH
400 ml
$3203
DIA2-PB011F-VK2
5 ml
$215
DIA2-PB011F-VNH
5 ml
$215
DIA2-PB001F
10 million/vial
$540
Plasma
Frozen
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Autoimmune: DIA2
Product Description
Lead Time
Fresh DIA2 WB - Whole blood is drawn from Type II Diabetes
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen DIA2 PB Plasma - Plasma is isolated from peripheral blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. Type II Diabetes peripheral blood plasma is obtained by
centrifugation of the Type II Diabetes PB.
Frozen DIA2 PB MNC - Peripheral blood mononuclear cells are
isolated from Type II Diabetes peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation, the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Diabetic Type II
PB MNC is available upon request.
Application
Product delivery is subject to stock availability.
Background
Diabetes Type II formally known as Non-insulin Dependent Diabetes
Mellitus or Adult Onset Diabetes, is a metabolic disorder that
is characterized by high blood glucose in the context of insulin
resistance and relative insulin deficiency. This is in contrast to
Diabetes Mellitus Type I in which there is an absolute insulin
deficiency due to destruction of islet cells of the pancreas.
Type II Diabetes makes up about 90% of cases of Diabetes with
the other 10% due primarily to Type I Diabetes and Gestational
Diabetes. Obesity is thought to be the primary cause of Type II
Diabetes in people who are genetically predisposed to the disease.
DIA2 specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
118
AUTOIMMUNE: INFLAMMATORY BOWEL DISEASE
CELLS & TISSUE
Inflammatory Bowel Disease Table of Contents
Crohn's Disease (CRO)
119
Ulcerative Colitis (UC)
120
Inflammatory Bowel Disease Cells & Tissue
AllCells sources samples of Inflammatory Bowel Disease (IBD) cells isolated from Crohn’s Disease and Ulcerative
Colitis patients. IBD plasma and peripheral blood mononuclear cells are available from each disease type. These are
available in frozen, fresh formats upon request.
Patient treatment history and clinical reports are available for each sample.
Donor Criteria & Screening
IBD cells and tissue products are obtained from volunteers participating in an Institutional Review Board (IRB) or
Human Subject Committee approved donor program. All donors sign an informed consent form and are aware that
these cells are used for research purposes. Donors must be over the age of 18.
Background
Inflammatory Bowel Disease is a group of inflammatory conditions of the colon and small intestine. The major types of
IBD are Crohn’s Disease and Ulcerative Colitis. IBD typically present with extra-intestinal manifestations such as liver
problems, arthritis, skin manifestations, and eye problems.
IBD may require immunosuppression to control symptoms such as prednisone, TNF inhibition, azathioprine,
methotrexate, or 6-mercaptopurine. Once the inflammation is successfully controlled, the patient is switched to a lighter
drug to keep the disease in remission.
IBD specimens can be used in preclinical research, drug discovery, drug toxicity, biomarker development, and preselection of indication for clinical trials.
For more information visit: www.allcells.com/ibd/
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
For In Vitro Research Use Only
119
Diseased Cells—Crohn's Disease (CRO)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
CRO-WB40-VK2
40 ml
$1418
CRO-WB100-VK2
100 ml
$1838
CRO-WB400-VK2
400 ml
$3203
CRO-WB400-VNH
400 ml
$3203
CRO-PB011F-VK2
5 ml
$215
CRO-PB011F-VNH
5 ml
$215
CRO-PB001F
10 million/vial
$540
Plasma
Fresh
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Autoimmune: Crohn's Disease
Product Description
Lead Time
Fresh CRO PB-Whole blood is drawn from Crohn's Disease
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen CRO PB Plasma - Plasma is isolated from pheriperal blood
collected into your choice of anticoagulant: EDTA(VK2) or sodium
heparin. Crohn's Disease peripheral blood plasma is obtained by
centrifugation of the PB.
Frozen CRO PB MNC - Peripheral blood mononuclear cells are
isolated from Crohn's Disease peripheral blood by diluting the whole
blood with PBS and using gradient separation techniques. After
centrifugation,the mononuclear cell layer is collected. MNC can be
processed further to isolate subpopulations. Fresh Crohn's PB MNC
is available upon request.
Application
CRO specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Background
Crohn’s Disease,also known as Regional Enteritis, is a type of
Inflammatory Bowel Disease that may affect any part of the
gastrointestinal tract from mouth to anus, causing a wide variety
of symptoms. Primarily, Crohn’s Disease causes abdominal pain,
diarrhea, vomiting, weight loss, and may also cause complications
outside the gastrointestinal tract such as skin rashes, arthritis,
inflammation of the eye, tiredness, and lack of concentration.
There is no cure for Crohn’s Disease and remission may not be
possible or prolonged, if achieved at all. In cases where remission is
possible, relapse can be prevented and symptoms controlled with
medication, lifestyle change, and in some cases surgery. Treatment
for Crohn’s Disease only occurs when symptoms are active and
involves first treating the acute problem then maintaining remission.
www.allcells.com
For In Vitro Research Use Only
120
Diseased Cells—Ulcerative Colitis (UC)—Whole Blood, Plasma,
Mononuclear Cells
Whole Blood
Product Format
Catalog #
QTY/Unit
Price/Unit
Fresh
UC-WB40-VK2
40 ml
$1418
UC-WB100-VK2
100 ml
$1838
UC-WB400-VK2
400 ml
$3203
UC-WB400-VNH
400 ml
$3203
UC-PB011F-VK2
5 ml
$215
UC-PB011F-VNH
5 ml
$215
UC-PB001F
10 million/vial
$540
Plasma
Fresh
Peripheral Blood Mononuclear Cells
Frozen
HLA-Typed Products are available upon request.
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Lead Time
Fresh UC PB - Whole blood is drawn from Ulcerative Colitis
patients in your choice of anticoagulant: EDTA(VK2) or sodium
heparin(VNH).
Frozen UC PB Plasma - Ulcerative Colitis peripheral blood plasma is
obtained by centrifugation of the PB. This product has a diminished
number of cells and platelets. Fresh Ulcerative Colitis PB plasma is
available upon request.
Frozen UC PB MNC - Peripheral blood mononuclear cells are
isolated from Ulcerative Colitis peripheral blood by diluting the
whole blood with PBS and using gradient separation techniques.
After centrifugation,the mononuclear cell layer is collected. MNC
can be processed further to isolate subpopulations. Fresh Ulcerative
Colitis PB MNC is available upon request.
Application
UC specimens can be used in:
• Pre-clinical research
• Drug discovery
• Drug toxicity
• Biomarker development
• Pre-selection of indication for clinical trials
ALLCELLS
Toll Free Phone: 1-888-535-3444
Product delivery is subject to stock availability.
Background
Ulcerative Colitis is a form of Inflammatory Bowel Disease of the
colon that includes characteristic ulcers or open sores. The main
symptom of active disease is usually constant diarrhea mixed with
blood of gradual onset. UC is an intermittent disease with periods of
exacerbated symptoms and periods that are relatively symptom-free.
Treatment is generally required for the disease to go into remission.
Ulcerative Colitis occurs in 35-100 people for every 100,000 in the
United States.
Standard treatment for UC depends on extent of involvement
and disease severity. The goal is to induce remission initially
with medications followed by the administration of maintenance
medications to prevent relapse of the disease.
www.allcells.com
For In Vitro Research Use Only
Autoimmune: Ulcerative Colitis
Product Description
121
ANIMAL PRIMARY CELLS & TISSUE
Animal Primary Cells Table of Contents
Non-Human Primate (NHP)
122
Non-Human Primate Cells & Tissue
Non-human primate cells are obtained from NIH-supported primate research centers. The blood or bone marrow is taken
from live normal/untreated non-human primates as a part of the research centers’ tissue program. AllCells offers blood and
bone marrow non-human primate products from the following species: M. fascicularis, M. nemestrina, or M.mulatta. None of
the aforementioned species are endangered or threatened by extinction.
For more information visit: www.allcells.com/non-human-primate/
PLEASE NOTE:
In order to have a dedicated team specifically focused on supporting Animal Cell Products, AllCells and Mae Huang,
an Animal Cell Products Specialist, recently established a new company, C&M LabPro, LLC. Mae has been working for
AllCells for many years. C&M LabPro now is the exclusive agent for AllCells animal cell products sales and
marketing. With 18 years of animal cell isolation experience and AllCells’ strong support, C&M LabPro continually
provides high quality products and services with lower prices for end-users.
Please note that AllCells will continue to service all Non-Human Primate Products listed on our catalog.
Starting 1/1/2016, C&M LabPro will be handling all other animal cell product inquiries and sales.
Please contact C&M LabPro for more information:
C&M LABPRO, LLC
3846 Railroad Ave, Pittsburg, CA 94565
Contact name: Mae Huang
Phone number: 1-877-630-0456
Email: mhuang@cmlabpro.com
ALLCELLS
Toll Free Phone: 1-888-535-3444
www.allcells.com
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122
Animal Cells—Non-Human Primate—Plasma/Mononuclear/CD34+ Stem Cells
Plasma
Product Format
Catalog #
QTY/Unit
Price/Unit
Frozen/PB-Plasma
NHP-PB001
5 ml
$87
Frozen/BM-Plasma
NHP-BM001
5 ml
$130
NHP-PB002
10 million/vial
$1472
NHP-BM002
10 million/vial
$1004
1 million/vial
$2531
Peripheral Blood Mononuclear Cells
Frozen
Bone Marrow Mononuclear Cells
Frozen
Bone Marrow CD34+ Stem/Progenitor Cells
Frozen
NHP-BM004
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
NHP-Plasma is processed through centrifugation to diminish cells
and platelets.
NHP-PB-MNC are enriched by density separation.
NHP-BM-MNC are isolated from bone marrow by diluting the
bone marrow with phosphate-buffered saline and using gradient
separation techniques. After centrifugation, the MNC layer is
collected. MNC can be processed to isolate subpopulations.
NHP-BM-CD34+ cells are isolated from NHP bone marrow
mononuclear cells using a direct immunomagnetic CD34+
MicroBead labeling system.
Related Products
Human BM-MNC
Human PB-MNC
Application
ALLCELLS
Toll Free Phone: 1-888-535-3444
Animal Cells: Non-Human Primate
NHP products can be used to study:
• Non-human primate lymphocyte and monocyte functions
• Evolutionary conservation and homology between human and
non-human primate lymphocytes and monocytes
• The homology of BM-CD34+ cells between humans and nonhuman primates, especially M. fascicularis
(cynomolgus) monkeys.
• The hematopoietic stem cell potential of the non-human
primate bone marrow CD34+ cells.
• Myeloid lineage differentiation and maturation
• Neutrophil transplantation potential
www.allcells.com
For In Vitro Research Use Only
123
CELL CULTURE PRODUCTS
Cell Culture Media, Cryopreservation Media and Cytokines
AllCells features basic and defined Cell Culture Media to suit your specific cell culture needs. In addition we have begun
manufacturing our own AllCells Cryopreservation Media, formulated to work best with our products. For most general
research purposes, we provide an array of the most commonly-used human cell culture media. For our specific cell
products, i.e. mesenchymal stem cells (MSCs), AllCells features defined media for the expansion and proliferation of these
cell types. See following pages for all of our Cell Culture products!
For more information visit: www.allcells.com/media/ and www.allcells.com/cytokines/
Basic Cell Culture Media:
Dulbecco's Modified Eagle's Medium (DMEM-001)
Dulbecco's Modified Eagle's Medium (DMEM-002)
Iscove's Modification of DMEM (IMDM-001)
Roswell Park Memorial Institute (RPMI-001)
Cytokines Continued:
Human Stem Cell Factor
Human Thrombopoietin
Defined Cell Culture Media:
MSC (adult stem cell culture):
MSC Basal Medium BM MSC, 500 ml (MSC-002)
MSC Stimulatory Supplement BM MSC, 50ml (MSC-003)
Macrophage:
Macrophage Basal Medium for Normal PB Monocyte Derived Macrophages, 450 mL (PB-MDM-002)
Macrophage Stimulatory Supplement for Normal PB Monocyte Derived
Macrophages, 50mL (PB-MDM-003)
Cryopreservation Media:
AllCells Cryopreservation Media (CRYO-001)
Cytokines:
Human Flt-3-Ligand
Human Granulocyte Stimulating Factor
Human Granulocyte Macrophage Stimulating Factor
Human Interleukin-3
Human Interleukin-4
Human Interleukin-6
Human Macrophage Colony Stimulating Factor
ALLCELLS
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124
Cell Culture Products—Basic Cell Culture & Cryopreservation Media
Cell Culture Media
Catalog #
QTY/Unit
Price/Unit
DMEM-001
500 ml/bottle
$24
DMEM-002
500 ml/bottle
$24
IMDM-001
500 ml/bottle
$33
RPMI-001
500 ml/bottle
$40
50 ml/bottle
$109
Cryopreservation Media
CRYO-001
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Dulbeco's Modified Eagle's Medium (DMEM-001) with 4.5g/L glucose, sodium pyruvate & L-glutamine. This media contains
L-glutamine, Sodium Bicarbonate and Sodium Pyruvate.
pH 7.2 + 0.2, Osmolality 335 + 30 mOsm
Dulbeco's Modified Eagle's Medium (DMEM-002 ) with 1g/L glucose, sodium pyruvate & L-glutamine. This media contains Lglutamine, Sodium Bicarbonate and Sodium Pyruvate.
pH 7.2 + 0.2, Osmolality 335 + 30 mOsm
Iscove's Modification of DMEM (IMDM-001 ) with L-glutamine & 25mM HEPES. This media contains L-glutamine, HEPES,
Sodium Bicarbonate and Sodium Pyruvate.
pH 7.2 + 0.2, Osmolality 265 + 310 mOsm
Roswell Park Memorial Institute (RPMI-001) with L-glutamine & HEPES. This media contains L-glutamine, HEPES, and Sodium
Bicarbonate.
pH 7.2 + 0.2, Osmolality 290 + 30 mOsm
AllCells Cryopreservation Media (CRYO-001) This media contains DMSO and proprietary reagents that increase the viability of
cryopreserved cells. Store at -80 C. This media has a shelf life of 3 months from the date of manufacture when stored properly. Avoid multiple freeze thaws of this media. Cryopreservation media is shipped on dry ice. MSDS available upon request.
Store at 2 - 80C. Protect from light. These products have a shelf-life of 18 months from the date of manufacture when stored properly. Avoid freezing of this media as it may cause precipitation upon thawing.
Shipping Information
Media is shipped at 40C on cold gel pack. MSDS available upon request.
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Cell Culture Products
Stability/Storage
125
Cell Culture Products—Defined Cell Culture Media
Mesenchymal Stem Cell Media
Catalog #
QTY/Unit
Price/Unit
MSC-002
500 ml/bottle
$76
MSC-003
500 ml/bottle
$263
PB-MDM-002
450 ml/bottle
$63
PB-MDM-003
50 ml/bottle
$399
Macrophage Media
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
Mesenchymal Stem Cell Basal Medium (MSC-002)-designed to support AllCells BM-MSC. MSC Basal Medium contains
L-glutamine, 110mg/L sodium pyruvate, and pyridoxine hydrochloride. This product does not contain growth factors and antibiotics.
The pH range is 7.1 to 7.5. MSC Basal Medium should be used in combination with AllCells MSC Stimulatory Supplement.
MSC Stimulatory Supplement (MSC-003)- designed to initiate and maintain growth of AllCells BM-MSC. This product
contains pre-selected Fetal Bovine Serum and supplements.
Macrophage Basal Medium (PB-MDM-002)- product contains L-glutamine, 110mg/L sodium pyruvate, and pyridoxine
hydrochloride. This product does not contain growth factors and antibiotics. pH is 7.1 to 7.5. The Macrophage Basal Medium
should be used in combination with AllCells Macrophage Stimulatory Supplement. This product is stable for one month after the
stimulatory supplement has been added and stored at 2 to 8°C.
Macrophage Stimulatory Supplement (PB-MDM-003)- designed to initiate and maintain growth of AllCells Monocyte
Derived Macrophages. This product contains pre-selected Fetal Bovine Serum and supplements.
Stability/Storage
Store at 2 - 80C. Protect from light. These products have a shelf-life of 18 months from the date of manufacture when stored properly. Avoid freezing of this media as it may cause precipitation upon thawing.
Shipping Information
Cell Culture Products
Media is shipped at 40C on cold gel pack. MSDS available upon request.
ALLCELLS
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126
Cell Culture Products—Cytokines
Human Flt-3 Ligand
Catalog #
QTY/Unit
Price/Unit
GF-FTL3L-2UG
2 ug/vial
$75
GF-FTL3L-10UG
10 ug/vial
$187
GF-FTL3L-100UG
100 ug/vial
$466
GF-G-CSF-2UG
2 ug/ vial
$75
GF-G-CSF-10UG
10 ug/vial
$186
GF-G-CSF-100UG
100 ug/vial
$603
Human Granulocyte Colony Stimulating Factor
Human Granulocyte Macrophage Stimulating Factor
GF-GM-CSF-5UG
5 ug/ vial
$75
GF-GM-CSF-20UG
20 ug/vial
$186
GF-GM-CSF-100UG
100 ug/vial
$466
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
AllCells Cytokines and Growth Factors are protein products used in tissue culture for human cell types. They can be utilized for
proliferation and/or differentiation of hematopoietic and immune cells.
Stability/Storage
Storage Prior to Reconstitution: -20ºC
Product Reconstitution: Sterile water at 100µg/mL.
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the
vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a
0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.
Shipping Information
ALLCELLS
Toll Free Phone: 1-888-535-3444
Cell Culture Products
Shipped at room temperature. MSDS available upon request.
www.allcells.com
For In Vitro Research Use Only
127
Cell Culture Products—Cytokines
Human Interleukin-3
Catalog #
QTY/Unit
Price/Unit
GF-IL-3-2UG
2 ug/vial
$75
GF-IL-3-10UG
10 ug/vial
$186
GF-IL-3-100UG
100 ug/vial
$466
GF-IL-4-5UG
5 ug/vial
$75
GF-IL-4-20UG
20 ug/vial
$186
GF-IL-4-100UG
100 ug/vial
$466
GF-IL-6-5UG
5 ug/vial
$75
GF-IL-6-20UG
20 ug/vial
$186
GF-IL-6-100UG
100 ug/vial
$466
Human Interleukin-4
Human Interleukin-6
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
AllCells Cytokines and Growth Factors are protein products used in tissue culture for human cell types. They can be utilized for
proliferation and/or differentiation of hematopoietic and immune cells.
Stability/Storage
Storage Prior to Reconstitution: -20ºC
Product Reconstitution: Sterile water at 100µg/mL.
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the
vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a
0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.
Cell Culture Products
Shipping Information
Shipped at room temperature. MSDS available upon request.
ALLCELLS
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128
Cell Culture Products—Cytokines
Human Macrophage Colony Stimulating Factor
Catalog #
QTY/Unit
Price/Unit
GF-M-CSF-2UG
2 ug/vial
$75
GF-M-CSF-10UG
10 ug/vial
$186
GF-M-CSF-100UG
100 ug/vial
$603
GF-SCF-2UG
2 ug/vial
$75
GF-SCF-10UG
10 ug/vial
$186
GF-SCF-100UG
100 ug/vial
$466
GF-TPO-2UG
2 ug/vial
$75
GF-TPO-10UG
10 ug/vial
$186
GF-TPO-100UG
100 ug/vial
$603
Human Stem Cell Factor
Human Thrombopoietin
Prices are subject to change without notice. Visit www.allcells.com for current pricing.
Product Description
AllCells Cytokines and Growth Factors are protein products used in tissue culture for human cell types. They can be utilized for
proliferation and/or differentiation of hematopoietic and immune cells.
Stability/Storage
Storage Prior to Reconstitution: -20ºC
Product Reconstitution: Sterile water at 100µg/mL.
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the
vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a
0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.
Cell Culture Products
Shipping Information
Shipped at room temperature. MSDS available upon request.
ALLCELLS
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For In Vitro Research Use Only
129
TECHNICAL NOTES
Technical Notes Table of Contents
•
How to Count Cells
130
•
How to Thaw Frozen Cells
131
•
How to Freeze Cell Products
132-133
•
How to Purify Bone Marrow MNC
134-135
•
How to Count Leuko Pak
136
•
How to Deplete Red Blood Cells
137
•
How to Passage & Subculture Cells:
•
-Mesenchymal Stem Cells
138
-Monocyte-Derived Macrophages
139
How to Isolate MNC Using a Ficoll Gradient
140
For more information including PDF versions of the Technical Notes and our BioTube Technical Videos visit:
www.allcells.com/support/how-to/
ALLCELLS
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130
Technical Notes—How to Count Cells
When you receive an AllCells product, please count the cells
IMMEDIATELY. AllCells is not liable for any cell loss during
subsequent processing. Please note: For Leuko Paks, please refer
If your cell count is significantly lower than what AllCells claims,
to "How to Count: Leuko Pak" page 136.
How to Count
In a biosafety hood, use a 5 mL pipet to mix the cell suspension
very well and to separate any cell clumps. For any cells in a 1.5 or 2
mL tube, mix cells with a P1000 pipet.
1. Check the cell suspension and see if there are any cell clumps.
If so, try to separate the cells using a P1000 pipet tip. Most of
the cell clumps should be small and easily separable.
2. The optimal cell concentration is 50 to 100 cells on one large
hemacytometer square (total 200 to 400 per hemocytometer).
Please make the proper dilution or adjust the concentration
and count again.
3. Immediately report any significant discrepancies to AllCells.
Take a 10 µL sample from the vial. Mix the 10µL sample with 10µL
of trypan blue. Dilute the cells if necessary and count the number of
cells in 4 quadrants of hemacytometer (see red quadrants in figure
below right) to determine the viability.
Table 1: Common Dilution Factors
N = # of cells counted on all 4 squares of a hemacytometer
Tube Size
Cell Number/
Unit Number
Cell
Sample
0.4%Trypan
Blue Amount
PBS (1x)
Dilution
Factor
1.5 to
2mL
0.5 to1 x106
10μL
10μL
---
2
5mL
5 x106
10μL
10μL
---
2
15mL
10 x106
10μL
10μL
---
2
15mL
15 to 20 x106
10μL
10μL
80μL
10
50 mL
30 to100 x106
10μL
10μL
80μL
10
Hemacytometer Method
Hausser BrightLine Directions for Use (http://www.
hausserscientific.com/hausserbrightlinedirect.htm)
Usage: cell counts
Cell Depth: 0.100mm+/- 2% (1/10mm)
Volume: 0.1 Microliter
Ruling Pattern: Improved Neubauer, 1/400 Square mm
d = dilution factor
Trypan Blue Method
Equation for Cell Count:
# of cells/vial = N / 4 x d x ____mL = _____x 104
Equation for Viability:
# of cells excluded by trypan blue / total number of cells
x 100% = ______%
Table 2: Common Dilution Factors for MNC Products with RBC
(Cord Blood and Bone Marrow Mononuclear Cell Products)
Tube Size
Cell Number/Unit
Number
Cell
Sample
*3% Acetic Acid with
Methylene Blue Solution
50 mL
100-200 x106
25 μL
475 μL
50 mL
200-400 x106
25 μL
475 μL
*StemCell Technologies, Inc., Vancouver; Cat. No. 07060, Tel: 604-877-0713
Neubauer Ruling
(Diagram credit: Hausser Scientific)
To clean the counting chamber:
After counting, remove the cover glass and clean the counting
chamber with water or a mild cleaning solution (10% solution of
bleach). Dry the counting chamber with a soft cloth or wipe, or rinse
with acetone.
For a technical video of this guide visit our BioTube page at:
www.youtube.com/user/AllCells
Methylene Blue Method
Equation for Cell Count:
# of cells/vial_____ / x 20 x d x ___mL = _____ x 106
NOTE: Methylene Blue Method will not give you the cell viability. To
find cell viability use Trypan Blue Method.
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Technical Notes
please take the following steps:
131
Technical Notes
Technical Notes—How to Thaw Frozen Cells
Shipping and Storage
For short-term storage (< 1 month), store in -80° C freezer. For
long-term storage (> 1 month), store in the “vapor phase” of a liquid
nitrogen tank.
General Medium Requirements
• IMDM, α-MEM, or RPMI-1640 containing 10% FBS
• DNase I
Thawing Cells
1. In a 37°C water bath, warm medium (IMDM, α-MEM, or RPMI1640) containing 10% FBS.
2. Clean the frozen vial with 70% alcohol before thawing. In a
biosafety hood, twist the cap a quarter-turn to relieve pressure,
and then retighten the cap.
3. In a 37°C water bath, quickly thaw the vial for approximately 2
minutes. Be careful not to submerge the entire vial in the water
bath. Do not remove the vial until a tiny ice-crystal is left.
4. Remove vial from water bath and clean the outside of the vial
with 70% alcohol.
5. In a biosafety hood, measure the cell suspension volume.
6. Count the cells using the Trypan Blue Method. Take a 10µl
sample from the vial. Mix 10µl of the sample with 10µl of
trypan blue. Dilute the cells if necessary and count the number
of cells on a hemacytometer to determine the viability.
N = # of cells counted on all 4 squares of a hemacytometer
d= dilution factor
Equation for Cell Count: # of cells/ vial = N / 4 x d x ____ml =
_____ x 104
Equation for Viability: # of cells excluded by trypan blue/ total
number of cells x 100% = ______%
7. (Optional) In order to avoid occasional clumping, when thawing
units with large cell numbers (>100x106), add DNase I to a
50ml conical tube.
a. To thaw purified cells for culture purpose, a total of 100µg
is needed.
b. To thaw mononuclear cells for cell culture purpose, a total of
300µg is needed.
Table 3: Typical Cell Suspension Volumes
Cell Unit
Cell Suspension Volume
< 1 x 106
1.2 ml
> 1x106
1.8 ml
9. Rinse the vial with 1ml of 37°C medium. In the 50ml conical
tube, slowly add the rinse drop by drop (5 seconds per drop) to
the cells while gently shaking the tube.
10. Next, slowly add the 37°C medium drop by drop (5 seconds
per drop) to the cells up to a total volume of 15 to 20 ml (about
10 times or more than the volume of the vial).
11. Centrifuge the cell suspension at 200xg at room temperature
for 15 minutes.
12. Using a pipet, carefully remove most of the supernatant (save
the supernatant in a second tube). Leave a few milliliters of the
supernatant behind so the cell pellet is not disturbed. Gently
resuspend the cell pellet in the remaining few milliliters of
medium.
Note: DMSO is heavier than medium. Try to aspirate and discard
the supernatant soon after centrifugation.
13. While gently shaking the tube, slowly add an additional 15 to
20ml of fresh medium to the tube.
14. Centrifuge the cell suspension at 200xg (1000 RPM on
Beckman Coulter™ Centrifuge) at room temperature for 15
minutes.
15. Using a pipet, carefully remove all but 2ml of supernatant.
Gently resuspend the cell pellet in the remaining 2ml of
medium and count. If the cell count is lower than expected,
centrifuge the supernatant saved in Step 11 at a higher speed,
count and combine if necessary.
16. Plate the cells in appropriate-sized dish/flask. Incubate in
a humidified incubator specified for tissue culture at 37°C
with 5% CO2. Your cells are now ready for downstream
applications.
For a technical video of this guide visit our BioTube page at:
www.youtube.com/user/AllCells
Note: DNase I should not be used if the cells are used for
purification of genomic DNA or if cDNA is for RNA synthesis.
8. Aseptically, using sterile techniques, transfer the cell
suspension to the 50ml conical tube, which contains DNase I.
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132
The below information is for customers who have extra fresh cell
products or have fresh cells that are not needed immediately for
processing. Please note once Cryopreservation Media is added
the process cannot be stopped and should be followed through
to completion. Freezing cells risks poorer viability and poorer cell
recovery after thaw.
the amount of IMDM required to resuspend cells using the
formulas:
How to Freeze Cell Products
(with the exception of bone marrow mononuclear cells)
Resuspend the cell pellet in this volume of cold (4oC) IMDM.
Reagent and Materials Required
•
Cryopreservation Media (80% FBS, 20% DMSO)
NOTE: for better results use AllCells Cryopreservation
Media CRYO-001 (see Procedure 1. below)
•
Isocove’s Modified Dulbecco’s Medium (IMDM)
•
Ice
Equipment Required
•
Refrigerated centrifuge
•
Biosafety hood (Class II)
•
Compound microscope
•
2mL Cryogenic vials
•
Freezing rack
•
1 to 10 mL pipets
•
Conical centrifuge tubes
•
Isopropanol graded freezing containers
•
-20oC freezer
•
-80oC freezer
a. # cells/desired concentration of cells per vial = # vials
needed
b. 0.9mL/vial X # vials = # mL of Cryopreservation Media
required
7. Mix the suspension well by pipetting up and down. Incubate
cells on ice for approximately 5 to 10 minutes.
8. In a small bowl, add ice and water to make a homogenous icewater bath.
9. Place the cell suspension tube in the ice-water bath. Add the
Cryopreservation Media to the cell suspension at a rate of 3 to
5 seconds per drop. Shake the tube constantly to ensure even
mixing.
10. Remove freezing rack from freezer and place in the hood. Mix
the cells well by pipetting and aliquot 1.8mL of cells to each
cryogenic vial.
11. Freeze cells at a controlled rate of 1oC/min by placing vials
in an alcohol-graded freezing container(s) and incubating at
-80oC for a minimum of 4 hours to a maximum of overnight.
Procedure
1. Thaw AllCells Cryopreservation Media (CRYO-001) with
proprietary blend of reagents to increase cell viability.
Alternately, prepare cryopreservation solution of 80% FBS and
20% DMSO up to 2 days in advance and store at 4oC.
12. Transfer vials to vapor phase of liquid-nitrogen storage.
Cells stored in the vapor phase can be stored long-term up to
several years. When commencing downstream applications on the
cells, follow the protocol on How to Thaw Cells by referring to the
Technical Note pg. 131.
For a technical video of this guide visit our BioTube page at:
www.youtube.com/user/AllCells
2. Label cryogenic vials with the appropriate vial and donor
information.
3. Place ice at the bottom of a freezing rack, place vials in the
freezing rack, store in a -20oC freezer for at least 10 minutes
until ready for use. .
4. Count the cells with preferred primary cell counting method
(see Technical Notes pg. 130)
5. Centrifuge cells at 200xg (1000 RPM on Beckman Coulter™
Centrifuge) for 5 minutes at 4oC and remove supernatant from
cell pellet.
6. Based on the desired concentration of cells/vial, calculate
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Technical Notes
Technical Notes—How to Freeze Extra Primary Cell Products
133
Technical Notes—How to Freeze Cell Products—Bone Marrow-MNC
Technical Notes
How to Freeze Bone Marrow MNC
Reagents Required
•
Washing Buffer: PBS (Ca2+ and Mg2+ free)
with 2mM EDTA and 10% FBS
•
CryostorTM CS5
•
Fetal Bovine Serum (FBS)
•
Ice
Procedure
1. Determine the number of vials needed and the
concentration of freeze.
a. Concentration of cells <1x106 freeze at 1.2ml
b. Concentration of cells >1x106 freeze at 1.8ml
2. Centrifuge the cells in a 50ml conical tube at 200xg at 4°C for
10 minutes without brake. Remove the supernatant.
Equipment Required
3. Use Cryostor™ CS5. Resuspend a small amount of Cryostor
CS5 with a P1000 pipettman. Bring the cell suspension to the
required amount for freezing.
•
Refrigerated centrifuge
•
Biosafety hood (Class II)
•
2mL Cryogenic vials
•
Compound microscope
•
1 to 10 mL pipets
•
Isopropanol graded freezing containers
•
-20oC freezer
•
-80oC freezer
ALLCELLS
4. Aliquot the appropriate amounts of cell suspension in the
cryogenic vials for freeze.
Toll Free Phone: 1-888-535-3444
5. Place vials in isopropanol graded freezing containers and place
in a -80 °C freezer. For long-term storage (>1 month), we
recommend moving vials to the vapor phase of liquid-nitrogen
storage.
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134
BM Unit Size
Total PBS (x1) Added to BM
For Shipment
3ml
1.5ml
10ml
4 ml
25ml
15ml
50ml
30ml
100ml
60ml
The BM-MNC population is approximately 20-30% of white blood
cells.
AllCells is not responsible for the recovery number of MNC or other
purified cells. For a specific number of a certain cell type please
consider our bone marrow purified products.
Reagents Required
•
Ficoll-Paque™ PLUS or Lymphocyte Separation Media
(1.077 density)
•
Washing Buffer: PBS (Ca2+ and Mg2+ free) with
2 mM EDTA and 10% Fetal Bovine Serum
•
Fetal Bovine Serum (FBS)
•
Ice
•
0.4% Trypan Blue (to check for dead cells)
•
3% Acetic Acid Methylene Blue
Equipment Required
Procedure
1. The whole BM product is shipped to you at room temperature.
It is highly recommended to store the whole BM product at
room temperature before MNC isolation.
2. Before MNC isolation, please count the whole BM product
with methylene blue to determine the WBC number in the
sample (please refer to How to Count Fresh Cells Technical
Notes).
3. Place the cell strainer on top of a sterile 50 ml conical tube.
Wet the strainer using 1 ml PBS. Slowly, run the BM through
the strainer. Wash the strainer with 5-10 ml of PBS (without
exceeding appropriate dilution ratio); discard the strainer.
4. Bring your BM cell suspension up to a 1:2 dilution (bone
marrow: PBS) in 50ml conical tubes. Dilute every 10ml of your
bone marrow product with 20ml of PBS.
5. Invert the tube several times. Carefully and slowly, under-lay
every 30ml of diluted blood with 14 ml of room temperature
Ficoll-Paque into each tube. Avoid creating air bubbles in the
Ficoll layer.
6. Centrifuge for 30 minutes at 400xg (1300 rpm for Beckman
Coulter™ Centrifuge) at 25°C in a swinging-bucket rotor
(no brake).
7. Remove the upper layer, leaving the mononuclear cell layer
undisturbed at the interphase.
8. Carefully collect the interphase cells and a small part of the
cells underneath the interphase.
•
Refrigerated centrifuge
•
Biosafety hood (Class II)
Note: Do not disturb the red pellet as this will cause granulocyte
contamination.
•
Hemacytometer
9. Transfer to a sterile 50 ml conical tube.
•
Compound microscope
•
5 to 10ml pipets
•
10 –200μl pipettors
•
10 – 200μl tips
•
50ml conical centrifuge tubes
•
40μm mesh nylon strainer (Becton Dickinson Biosciences,
REF 3523)
10. Centrifuge your sample at 1700xg (3000 rpm for Beckman
Coulter™ Centrifuge) at 4°C for 10 minutes, no brake.
The following process:
•
Should be performed in a sterile biosafety hood (Class II)
to avoid bacterial contamination.
•
Is written for MNC isolation using 50ml conical centrifuge
tubes.
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Toll Free Phone: 1-888-535-3444
11. Remove the supernatant. Resuspend the cells with 5 ml of cold
(4°C) Washing Buffer, using a 5ml pipet.
12. Fill the tube up to 50ml with cold Washing Buffer.
13. Count the cells using the Methylene Blue Method (See "How to
Count Fresh Cells").
14. Centrifuge at 200xg (1000 rpm for Beckman Coulter™
Centrifuge) at 4°C for 10 minutes, no brake.
15. Resuspend the cell pellet with proper culture media and count
the cells. Adjust the MNC concentration with proper culture
media and proceed to your experiment.
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Technical Notes
Technical Notes
Technical Notes—How to Purify Bone Marrow MNC
135
Technical Notes—How to Purify Bone Marrow MNC (continued)
Technical Notes
Frequently Asked Questions
Q. Can bone marrow cells be used in colony formation assays?
A. Yes, our purified bone marrow CD34+ cells have been used in
colony formation assays, and they perform extremely well.
Q. After ficolling, my BM-MNC have many red blood cells (RBC). Is
this normal? If so, how do I get rid of them?
A. It is extremely difficult to collect only MNC during ficolling.
The amount of RBC you harvest depends on donor variation and
technique. You can use Ammonium Chloride for RBC lysis.
Note: Excessive exposure to ammonium chloride can potentially
damage leukocytes.
Q. How do I get rid of clumps in my mononuclear cells?
A. You can use a 40 μm cell strainer to strain the clumps out.
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136
Blood is obtained from normal healthy volunteers with collection
of maximum three-blood volume on COBE SpectraTM apheresis
machine.
6. Take 25µl sample of blood and place into 1.5 ml centrifuge
tube with 475 µl Methylene Blue.
Reagent and Materials Required
7. Add 80µl of DPBS into a mixing well (rounded non-sterile 96
well plate). Invert the 1.5 ml tube several times to mix the blood
and Methylene Blue thoroughly.
•
Sterile Room Temperature Washing Buffer: 500mL DPBS
with 2mM EDTA
•
Cold Washing Buffer: 500mL DPBS+10mL Fetal Calf
Serum (FCS) or Fetal Bovine Serum (FBS) + 2mM EDTA
•
Cold Shipping Buffer: 500mL DPBS+8mL Bovine Serum
Albumin (BSA; 0.5%)
•
Ammonium Chloride Solution (0.8% NH4Cl with 0.1mM
EDTA). Use approximately 10 to 15ml of ammonium
chloride per 2-5x108 MNC per tube.
•
50mL Conical Tubes
•
5mL and 25mL pipets
•
Latex gloves
9. Count the total number of mononucelar cells using Methylene
Blue Method.
a. Methylene Blue Method Equation for Cell Count:
# of cells/vial_____ / 20 x d x ___mL = _____ x 106
b. To assess viability, use Trypan Blue Method. Mix equal
volumes of cell to trypan blue (10µl:10µl) and dilute
appropriately where necessary.
Equipment Required
•
Class II Biological Safety Cabinet
•
Beckman Centrifuge
•
Pipettors
•
Stainless Steel Surgical Scissors
•
Stainless Steel Clamps
Equation for Viability:
# of cells excluded by trypan blue/ total number of cells x
100% = ______%
To Process PB-MNC from Leuko Pak
1. Count the cells from Leuko pak as detailed above.
Safety Precautions
•
•
•
8. Take a 20µl sample from the 1.5 ml tube and mix with 80µl
DPBS. Load the hemacytometer with 20 µl of diluted sample.
Protective gloves should be worn at all times.
Use a safety pipetting device for all pipetting.
NEVER PIPET BY MOUTH
2. Refer to "How to Deplete RBC," page 137.
Procedure
1. Upon receipt of the apheresis blood, verify that the product
label is correct and donor information sheet is provided.
2. Remove the blood bag from biohazard bag. Place the
apheresis bag in a Biological Safety Hood and examine the
bag for leaks or clumps.
3. Mix the blood bag well and locate the blood transfer line.
4. Disinfect the blood transfer line with an alcohol swab and
cut using sterile scissors.
5. Wipe the cut blood transfer line with an alcohol swab.
Wait 1 minute until any remaining alcohol has evaporated.
Carefully place 5ml of the blood in a sterile 50ml conical
centrifuge tube and clamp the transfer pack to prevent any
spills.
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Technical Notes
Technical Notes—How to Count: Leuko Pak
137
Technical Notes
Technical Notes—How to Deplete Red Blood Cells
This protocol is designed for human peripheral blood, bone marrow and cord blood for red blood cells (RBC) preparations. This protocol
outlines the procedure for depletion of RBC from whole blood and from mononuclear cells.
Procedure For RBC Lysis from Mononuclear Cells
Materials and Reagents
•
Dulbecco’s Phosphate Buffered Saline (DPBS) with 2mM
EDTA
•
WB (washing buffer, 2% Fetal Bovine Serum (FBS), 2mM
EDTA in DPBS)
•
Ammonium Chloride (StemCell Technologies Cat# 07850)
Refrigerated centrifuge
•
Ice in a bucket
•
Pipettor
•
Biosafety hood
2. Discard the supernatant and resuspend cells with 25 ml
of WB.
3. Centrifuge the tube at 200xg (1000 rpm) for 10 minutes at
room temperature.
Equipment
•
1. Centrifuge the cells after collecting the lymphocyte layer from
the separation medium.
4. Discard the supernatant and add the Ammonium Chloride at
4:1 ratio. For example, 4ml of Ammonium Chloride to 1ml
of blood.
5. Mix the cells and Ammonium Chloride and place on ice for 10
minutes to allow lysis of the RBC.
Safety Precautions
•
Protective gloves should be worn at all times
•
Use a safe pipetting device for all pipetting
•
NEVER PIPET BY MOUTH
Procedure For Lysis of RBC From Whole Blood
6. Fill the tube with WB up to 25ml and centrifuge the tube at
200xg (1000rpm) for 10 minutes at 4oC.
7. Discard the supernatant and wash the cells twice with WB.
The cells are ready for use.
1. Transfer 25ml of blood into a 50ml conical tube and centrifuge
at 200xg (or 1000 rpm on Beckman Coulter™ Centrifuge) in a
swinging bucket rotor for 10 minutes at room temperature.
2. Discard the supernatant and resuspend the blood with 25ml of
DPBS (containing 5mM EDTA) and centrifuge at 200xg (1000
rpm) for 10 minutes at room temperature.
3. Discard the supernatant and add the Ammonium Chloride at
9:1 ratio. For example, 9ml of Ammonium Chloride to 1ml of
blood. You should have 45ml of Ammonium Chloride to 5ml
of blood.
4. Mix the cell and Ammonium Chloride and place on ice for 10
minutes to allow lysis of the RBC.
5. Centrifuge the tube at 500xg (1500rpm) for 10 minutes in
4oC.
6. Discard the supernatant and wash the cells twice with WB. The
cells are ready for use.
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138
Thawing and Culturing Cells
1. Calculate the number of flasks needed. The recommended
seeding density for AllCells BM Mesenchymal Stem Cells
(MSC) is 5,000 to 10,000 cells/cm2. For example, one vial of
AllCells MSC (7.5x105/vial) would be sufficient for 1 to 2 T75
flasks or 3 to 6 T25 flasks.
2. Prepare the culture media:
a. Add 2mM concentration of L-glutamine and the entire
contents of 50ml AllCells Stimulatory supplement (Cat.
No.MSC-003)
to 450ml of AllCells Basal Medium (Cat. No. MSC-002).
b. Add 2 to 3 ml/5 cm2 of the mixed media into the T75 or
T25 flasks.
c. In a 37°C, 5% CO2 humidified incubator allow the flasks to
equilibrate in for at least 30 minutes.
3. Wipe the frozen vial with 70% alcohol before thawing. In a
biosafety hood, briefly twist the cap a quarter-turn to relieve
pressure, then retighten the cap.
4. In a 37°C water bath, quickly thaw the vial. Be careful not to
submerge the entire vial in the water bath. Do not remove the
vial until a tiny ice-crystal is left. Wipe the outside of the vial
with 70% alcohol.
5. In a biosafety hood, take a 10ul sample from the vial. Mix
the 10µl sample with 10µl of trypan blue. Dilute the cells if
necessary and count the number of cells on a hemacytometer
to determine the viability.
N = # of cells counted on all 4 squares of a hemacytometer
d= dilution factor
Equation for Cell Count:
# of cells/ vial = N/4 x d x____ml = _____104
Equation for Viability:
# of cells excluded by trypan blue/ # total number of cells x 100%
= ______%
Note: Avoid repeated warming and cooling of the medium. If the
entire contents are not needed for a single procedure, transfer only
the required volume to a sterile secondary container.
Subculturing Cells
1. Subculture the cells when they are 70 to 80% confluent
(about 10 days). Many mitotic figures should be visible
throughout the flask.
2. For each 25cm2 of cells to be subcultured:
a. Prepare 2 ml of Trypsin/EDTA, 5 ml of HEPES Buffered
Saline Solution (HEPES-BSS), 4 ml of Trypsin Neutralizing
Solution (TNS such as 10% FBS-DPBS). Allow the
aforementioned items to come to room temperature.
b. From one culture flask, remove the medium and rinse the
flask with 5 ml of room temperature HEPES-BSS. (This is
an IMPORTANT step. The culture medium contains complex
proteins that neutralize the effect of trypsin.) Remove the
HEPES-BSS from the flask.
c. Repeat Step b for each culture flask.
3. Add 2 ml of Trypsin/EDTA solution to each T25 flask. Allow
the trypsinization to continue until approximately 90% of the
cells have lifted up from the T25 flask. This entire process
takes about two to six minutes. Hit the flask against the palm
of your hand to release the majority of cells from the culture
surface. If only a few cells detach, wait 30 seconds and repeat
this motion again. If cells still do not detach, wait and repeat
every 30 seconds thereafter.
4. After cells have detached, neutralize the trypsin in the flask
with 4 ml of room temperature Trypsin Neutralizing Solution.
5. Quickly transfer the detached cells to a sterile 15ml centrifuge
tube and rinse the flask with a final 2 ml of HEPES-BSS to
collect residual cells. Add this rinse to the centrifuge tube.
**This is a very important step to determine if the cell viability
number matches what AllCells claims.
Maintaining Cell Culture
1. Change the growth medium the day after seeding and every
other day thereafter.
2. In a sterile container, warm an appropriate amount of Complete
Medium to 37°C.
3. Remove the medium and replace with the warmed, fresh
medium; return the flask to the incubator.
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Technical Notes
Technical Notes—How to Passage and Subculture—
Mesenchymal Stem Cells
139
Technical Notes
Technical Notes—How to Passage and Subculture—
Monocyte-Derived Macrophages
General Media Requirements
N = # of cells counted on all 4 squares of a hemacytometer
d= dilution factor
Monocyte Derived Macrophages (MDM) will mature if cultured with
Macrophage Basal Medium (Cat. No. PB-MDM-002) in conjunction
with Macrophage Stimulatory Supplement (Cat. No. PB-MDM-003).
Equation for Viability:
# of cells excluded by trypan blue/ # total number of cells x
100% = ______%
Types of Culture Vessels
• 96 well plate
• 35mm tissue culture dish
• 100mm tissue culture dish
• T25 flask
• T75 flask
**This is a very important step to determine if the cell viability
number matches what AllCells claims.
Thawing and Culturing Cells
1. Calculate the number of culture vessels needed. The
recommended seeding density for AllCells MDM is 10x104
cells/cm2. For example, one vial of AllCells MDM (1.5x106/vial)
would be sufficient for six T25 flask or two 100mm dish.
2. Prepare the Complete Culture Medium: Add 2mM
concentration of L-glutamine, 1% of the antibiotic-antimycotic
stock solution and the entire contents of 50ml AllCells
Stimulatory supplement (Cat. No. PB-MDM-003) to 450ml of
AllCells Basal Medium (Cat. No. PB-MDM-002).
3. Wipe the frozen vial with 70% alcohol before thawing.
4. In a biosafety hood, briefly twist the cap a quarter-turn to
relieve pressure, then retighten the cap.
5. In a 37°C water bath, quickly thaw the vial. Be careful not to
submerge the entire vial. Do not remove the vial until a tiny
ice-crystal is left.
6. Wipe the outside of the vial with 70% alcohol.
7. Count cells by taking 10μl from the vial to mix with 10μl of
trypan blue solution. Count cells with a hemacytometer .
ALLCELLS
Equation for Cell Count:
# of cells/ vial = N/4 x d x____ml = _____104
Toll Free Phone: 1-888-535-3444
8. Initiate the culture. Resuspend and dispense 10,000 cells/cm2
from the vial into the culture vessel.
9. Add the desired volume of prepared culture medium to the
vessel.
10. Gently rock the culture vessel to evenly distribute the cells
and place in 5% CO2 incubator.
Note: It is not necessary to wash the cells because this action is
more damaging than the effects of DMSO residue in the culture.
Maintaining Cell Culture
1. Change the growth medium the day after seeding and
every other day thereafter.
2. In a sterile container, warm an appropriate amount of complete
culture medium to 37°C. Remove the medium and replace it
with the warmed, fresh medium
3. Return the flask to the incubator.
Note: Avoid repeated warming and cooling of the medium. If
the entire contents are not needed for a single procedure, transfer
only the required volume to a sterile secondary container.
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140
AllCells is not responsible for the recovery number of MNC or other
purified cells. For a specific number of a certain cell type please
consider our purified products.
Reagents Required
• Ficoll-Paque™ PLUS or Lymphocyte Separation Media
• (1.077 density)
•
Washing Buffer: PBS (Ca2+ and Mg2+ free) with
• 2 mM EDTA
• Ice
• 0.4% Trypan Blue (to check for dead cells)
• 3% Acetic Acid Methylene Blue
Equipment Required
• Refrigerated centrifuge
• Biosafety hood (Class II)
• Hemacytometer
• Compound microscope
• 5 to 10ml pipets
• 10 –200μl pipetman
• 10 – 200μl tips
• 50ml conical centrifuge tubes
• 40μm mesh nylon strainer (Becton Dickinson Biosciences,
REF 3523)
The following process:
• Should be performed in a sterile biosafety hood (Class II)
to avoid bacterial contamination.
• Is written for MNC isolation using 50ml conical centrifuge
tubes.
Coutler™ Centrifuge) at 25°C in a swinging-bucket rotor(no
brake).
6. Remove the upper layer, leaving the mononuclear cell layer
undisturbed at the interphase.
7. Carefully collect the interphase cells and a small part of the
cells underneath the interphase.
Note: Do not disturb the red pellet as this will cause granulocyte
contamination.
8. Transfer to a sterile 50 ml conical tube.
9. Centrifuge your sample at 1700xg (3000 rpm for Beckman
Coutler™ Centrifuge) at 4°C for 10 minutes, no brake.
10. Remove the supernatant. Resuspend the cells with 5 ml of cold
(4°C) Washing Buffer, using a 5ml Pipetman.
11. Fill the tube up to 50ml with cold Washing Buffer.
12. Count the cells using the Methylene Blue Method (See "How
to Count Fresh Cells").
13. Centrifuge at 200xg (1000 rpm for Beckman) at 4°C for 10
minutes, no brake.
14. Resuspend the cell pellet with proper culture media and count
the cells. Adjust the MNC concentration with proper culture
media and proceed to your experiment.
For a technical video of this guide visit our BioTube page at:
www.youtube.com/user/AllCells
Procedure
1. The whole tissue product is shipped to you at room
temperature. It is highly recommended to store the whole
tissue product at room temperature before MNC isolation.
2. Before MNC isolation, please count the whole tissue product
with methylene blue to determine the WBC number in the
sample (please refer to How to Count Fresh Cells Technical
Notes).
3. Bring your tissue cell suspension up to a 1:2 dilution in 50ml
conical tubes. Dilute every 10ml of your tissue product with
20ml of PBS.
4. Invert the tube several times. Carefully and slowly, under-lay
every 30ml of diluted blood with 14 ml of room temperature
Ficoll-Paque into each tube. Avoid creating air bubbles in the
Ficoll layer.
5. Centrifuge for 30 minutes at 400xg (1300 rpm for Beckman
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Technical Notes
Technical Notes—How to Isolate MNC Using a Ficoll Gradient
141
Index: Alphabetical
INDEX - ALPHABETICAL
A
Acute Lymphoid Leukemia (ALL) 93-94
Bone Marrow 94
CD19+ B Cells 94
Mononuclear Cells 94
Plasma 94
Peripheral Blood 93
CD19+ B Cells 93
Mononuclear Cells 93
Plasma 93
Acute Myeloid Leukemia (AML) 95-96
Bone Marrow 96
CD34+ Cells 96
Mononuclear Cells 96
Plasma 118
Peripheral Blood 95
Mononuclear Cells 95
Plasma 95
Animal Primary Cells 121-122
Apheresis Collection (Leuko Pak) 6, 16-18, 33, 37, 59
Autoimmune Diseased Cells 108-120
B
B Cells 39-40, 80, 93-94
Basophils 50
Bioservices 19-27
Bone Marrow (Human, Healthy) 16-18, 65-72
Aspirates 16-18, 66
CD34-Depleted Mononuclear Cells 68
Mononuclear Cells 67
Myeloid Cells
CD36+ Early Erythroid Progenitor Cells 70
Stem/Progenitor Cells - CD34+ Cells 69
Mesenchymal Cells 72
CD105+ Endothelial Cells 71
Mesenchymal Stem Cells 72
Whole 66
C
CD133+ Cells 63
CD34+ Cells 38, 62, 69, 96, 100, 122
Cell-based Assays 22-23
Cell Culture Media 123-128
Cell Separation Methods 11
Chronic Lymphoid Leukemia (CLL) 97-98
Bone Marrow 98
CD19+/CD5+ B Cells 98
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Mononuclear Cells 98
Plasma 98
Peripheral Blood 97
CD19+/CD5+ B Cells 97
Mononuclear Cells 97
Plasma 97
Chronic Myeloid Leukemia (CML) 99-100
Bone Marrow 100
CD34+ Cells 100
Mononuclear Cells 100
Plasma 100
Peripheral Blood 99
Mononuclear Cells 99
Plasma 99
Cord Blood 73-85
CD34-Depleted Mononuclear Cells 76
Lymphoid Cells
CD3+ Pan T Cells 81
CD4+/CD45RA+ Naïve T Cells 84
CD4+ Helper T Cells 82
CD8+ Cytotoxic T Cells 83
CD19+ B Cells 80
CD56+ NK Cells 85
Mononuclear Cells (MNC) 75
Myeloid Cells
CD14+ Monocytes 79
CD36+ Early Erythroid Progenitor Cells 78
Plasma 74
Stem/Progenitor Cells
CD34+ Cells 77
Crohn's Disease Cells 119
Cryopreservation Media 124
Custom Products/Projects 17-18, 28-30, 90
Culture Media 124-125
Cytokines 126-128
FTL-3L 126
G-CSF 126
GM-CSF 126
IL-3 127
IL-4 127
IL-6 127
M-CSF 128
SCF 128
TPO 128
Cytotoxic T Cells 46-47, 83
D
Dental Pulp Stem Cells 87
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142
Alphabetical Index
E
Early Erythroid Progenitor Cells (CD36+) 70, 78
ELISA, ELISpot 22
Endothelial Cells 71
Eosinophils 51
Erythrocytes 53
F
Flow Cytometry 24
Fluorescence-activated Cell Sorting (FACS) 24
Follicular Lymphoma (FL) 106-107
G
G-CSF Mobilized Cells 11, 58-59
H
Helper T Cells 42, 42, 82
Hematopoietic Assays 23
HLA-Typing 27
How To Count Cells 130
How To Count Leuko Pak 136
How To Freeze Cells Products 132-133
How To Isolate MNC Using a Ficoll Gradient 140
How To Passage and Subculture Cells 138-139
How to Purify Bone Marrow MNC 134-135
How to Thaw Frozen Cells 131
I
Immunoassays 22
Immunophenotyping 22
In vitro proliferation/inhibition assays 25
Inflammatory Bowel Diseased Cells 118-120
ISHAGE 23
L
Leukemia Cells 91-100
Leuko Pak (Apheresis Collection) 33, 59
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Leukocytes 51-52
Lupus (SLE) Diseased Cells 115
M
Macrophages 57, 139
Mantle Cell Lymphoma (MCL) 106-107
Media 123-128
Memory T Cells 45
Mesenchymal Stem Cells (MSC) 72, 87, 138
Mobilized Peripheral Blood 58-64
CD34-Depleted Mononuclear Cells 61
Leuko Pak 59
Mononuclear Cells 60
Myeloid Cells
CD14+ Monocytes 64
Stem/Progenitor Cells
CD34+ Cells 62
CD133+ Cells 63
Molecular Services 26
Monocytes 55-57, 64, 79
Mozobil 11, 17, 59
Multiple Myeloma (MM) 101-103
Bone Marrow 103
Mononuclear Cells 103
Plasma 103
Peripheral Blood 102
Mononuclear Cells 102
Plasma 102
Multiple Sclerosis (MS) Diseased Cells 111
Myelodysplastic Syndrome Diseased Cells 104-105
N
Natural Killer Cells 49, 85
Neutrophils 52
Non-Hematopoietic Stem Cells 86-87
Non-Hodgkin’s Lymphoma 106-107
Non-Human Primate Cells 122
Normal Peripheral Blood 32-57
Leuko Pak 32-33
Lymphoid Cells
BDCA4+ Lymphoid/Plasmacytoid Dendritic Cells 48
CD4+/CD25+ Regulatory T Cells 43
CD4+/CD45RA+/CD25- Naïve T Cells 44
CD4+/CD45RO+ Memory T Cells 45
CD4+ Helper T Cells 42
CD8+/CD45RA+ Naive Cytotoxic T Cells 47
CD8+ Cytotoxic T Cells 46
CD19+ B Cells 39
CD19+/IgD+ Naive B Cells 40
CD56+ NK Cells 49
Pan T Cells 41
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Index: Alphabetical
Delivery Information 5-8
Dendritic Cells 48, 56
Diffuse Large B Cell Lymphoma (DLBCL) 106-107
Diseased Cells 88-120
DNA Analysis 26
143
Alphabetical Index
Index: Alphabetical
Mononuclear Cells 37
Myeloid Cells
Basophils 50
CD14+ Monocytes 55
Eosinophils 51
Mature Erythrocytes (RBC) 53
Monocyte Derived Immature Myeloid Dendritic Cells 56
Monocyte Derived Macrophages 57
Neutrophils 52
Platelets 54
Plasma 36
Stem/Progenitor CD34+ 38
Whole Blood 34-35
O
Ordering Information 5-8, 9
Osteoarthritis Diseased Cells 112
P
Plasmacytoid Dendritic Cells 48
Platelets 54
Psoriasis (PSO) Diseased Cells 113
R
Red Blood Cells 53
Rheumatoid Arthritis Diseased Cells 114
S
Storage information 7
T
T Cells 41-47, 81-84
Technical Notes 129-140
Technical Support 9
Terms & Conditions 12-14
Tissue Collection Facility 15-18
Tissue Culture Services 25
Toxicology Studies 25
Type I Diabetes Diseased Cells 116
Type II Diabetes Diseased Cells 117
U
Ulcerative Colitis Diseased Cells 120
W
Waldenström’s Macroglobulinemia (WM) 106-107
Website Information 9
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144
NOTES
ALLCELLS
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145
QUICK REFERENCE GUIDE
Donor Criteria
•
Age: 18-55 years old (18-40 years old for mobilized donation)
•
Donors cannot be pregnant
•
Weight: >110 lbs for 25mL and 50mL BM donations
•
Donors are screened for HIV, Hepatitis B and Hepatitis C
•
Weight: >130 lbs for 100mL BM donations
•
Weight: >140 lbs for Normal and Mobilized Leuko Pak donation
•
CBC lab tests must meet protocol specifications
•
Donors must be in general good health. Donors with any of the
before donation
•
Donors are required to sign a procedure-specific consent form
prior to the collection procedure
•
following will be excluded from donating:
All consents and protocols have been approved by an
independent biomedical review board
–History of heart, lung, liver, kidney disease (including asthma)
•
Minimum 12 months between mobilized donations
–Blood and bleeding disorders, including sickle cell disease
•
Twice in a lifetime maximum for mobilized donations
–Neurologic disorders
–Cancer
–Diabetes
–Positive: HIV, HbsAg, HCAb
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146
Quick Reference Guide
Cell Subset Products
The table below is for reference purposes only. Please note that the average percentage of cell subsets is referring to the recovered MNC population and
not the total white blood cell population.
Cells
Normal Peripheral
Blood
Mobilized Peripheral
Blood- Reg E
Bone Marrow
Cord Blood
Whole Blood
CD34+
29 %
0.83%
0.73 %
0.58 %
0.55 %
CD133+
0.09 %
0.23 %
1%
0.21 %
0.4 %
CD33
29 %
33 %
27 %
21 %
9%
CD105+
0.41 %
2%
0.89 %
1%
1%
+
CD56
2%
3%
1%
2.6 %
1%
CD19+
1%
10 %
2%
1%
1%
+
CD16
4%
8%
30 %
33 %
39 %
CD15+
4%
16 %
63 %
40 %
44 %
CD14
16 %
22 %
4%
12 %
6%
CD8+
23 %
19 %
6%
10 %
9%
CD4
3%
24 %
3%
21 %
12 %
CD3+
60%
45 %
27 %
28 %
43 %
10%
10%
+
+
+
CD4 /CD45RA
+
35 %
+
Basophils
0.3%
Neutrophils and
Eosinophils
0.2%
Granulocytes
RBC
<0.01%
<0.01%
<0.01%
Platelets
<0.01%
<0.01%
<0.01%
CD138+ Plasma Cells
0.1-0.4%
We cannot guarantee the recovery of the cell types listed above. For a specific number of a certain cell type, please consider our purified products.
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CellSystems
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