University Of Florida
Bloodborne Pathogen Program
Standard Operating Procedures
Revised February 9, 2011
Updated (annually)
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BBP Standard Operating Procedures
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University Of Florida
Bloodborne Pathogen Program
Standard Operating Procedures
Please use the enclosed worksheets to create standard operating procedures
(SOPs) for individual laboratories or work areas. These pages are intended to be
photocopied and used as a means of compliance with the bloodborne pathogen
standard. These worksheets will assist you in tailoring the Exposure Control
Plan to your individual needs. Please note that they are to be used in conjunction
with the UF Exposure Control Plan.
Keep the completed worksheets with the Exposure Control Plan in a location
that is accessible to your employees. EH&S will monitor individual laboratories
for the presence of SOPs. There is no need to send the completed sheets to
EH&S.
Table of Contents
Facility Identification Sheet ............................................................................................ 3
JOBS: Exposure Determination List .............................................................................. 4
TASKS: Exposure Determination List ........................................................................... 5
HBV Vaccine ..................................................................................................................... 6
Exposure Management ................................................................................................... 7
Protective Equipment Used ............................................................................................ 8
Decontamination Schedule ............................................................................................. 9
Engineering Controls Check ........................................................................................ 10
Biomedical Waste Plan .................................................................................................. 11
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University of Florida
Exposure Control Plan
Standard Operating Procedures
Facility Identification
Facility Name____Neurochemistry Core _________________________
Facility Room & Building #______ P2-32 & P2-41__Pharmacy Building 445_____
Mailing Address___JHMC Box 10487______________________________________
Phone Number______273-6311 or 846-1354_________________________________
Plan Prepared By________Dr. William J. Millard__________
Date____ February 18, 2009______________________________
Date of Review/Update_________ February 9, 2011___________
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Signature of supervisor
__William J. Millard_________________________________________________
Name of supervisor (PLEASE PRINT OR TYPE)
_Pharmacodynamics_______________________________
Department (PLEASE PRINT OR TYPE)
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Exposure Determination List
Jobs
The following persons and job classifications in this facility may have contact
with blood or other potentially infectious materials. These people are in the UF
BBP program. (Please use official UF job classification titles).
NAME
JOB CLASSIFICATION
William J. Millard__________________ ___Professor_____________________
Yun-Ju He_________________________ ___Senior Biological Scientist_______
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Exposure Determination List
Tasks
The following procedures performed in this facility may result in occupational
exposures to blood or other potentially infectious materials. (Please list all tasks
in which exposure may occur).
Radio-immunoassay Procedures______ _________________________________
Sample Pipetting and aliquotting______ _________________________________
Enzyme linked Immunosorbant Assay_
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Hepatitis B Vaccine
Write in the names of your employees, with the date they were offered the
hepatitis B vaccine.
The following at-risk employees have been offered the hepatitis B vaccine free of
charge:
Name
Date provided
Date declined
William J. Millard_______________________
1/94, 2/94, 7/94
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Yun-Ju He______________________________
6/93, 10/94, 8/96
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Exposure Management
In the event of an exposure to human blood or other potentially infectious
materials, employees of this facility shall do the following (please fill in specifics
for this work area):
1.
Wash skin and wound exposures with soap and water:
Wash for at least 5 minutes with alternate soap and water rinse.
2.
Wash eye and mucous membrane exposures in running water for 15
minutes:
Eye wash station in the lab
3.
Report exposures to the supervisor:
Dr. William J. Millard, Room 4-334, HPNP , Phone # 273-6311 or 8718385
4.
Individuals exposed to human blood or other potentially infectious
materials shall go immediately (within one hour) to the following location
for treatment:
(866) 477-6824 in the Gainesville area Only.
This Needle Stick hotline is manned 24 hours a day. You will
be directed from there were to go for further treatment
5.
In All other Locations:
Individuals exposed to human blood or other potentially infectious
materials shall go immediately (within one hour) to the following location
for treatment.
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Protective Equipment
(by task)
Task
Personal Protective Equipment Used
Aliquotting and pipetting samples
Gloves goggles, gowns
Vortexing Samples
Gloves goggles, gowns
Centrifugation of samples
Gloves goggles, gowns
Aspiration of Samples
Gloves goggles, gowns
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Equipment/Worksite Decontamination Schedule
for
Neurochemistry Core RIA Facility
Facility
Pharmacy Building #455 – Room P2-41
Location
Equipment
Schedule
Pipettors
End of experimental day
Gamma Counter
End of experimental day
Assay Racks
End of experiment
Pipette tips
End of experiment
Centrifuges
End of experiment
RIA disposables
End of experiment
Lab Benches
End of experiment
Aspirator
End of experiment
BBP Standard Operating Procedures
Procedure
Wipe outside and clean inside with 1:100
bleach solution.
Wipe outside with detergent solution
Soak overnight in 1:10 bleach solution,
wash with count-off detergent
Discard in red biohazard bag
Wipe outside and clean inside with 1:100
bleach solution.
Soak overnight in 1:10 bleach solution,
discard in radioactive waste.
Discard bench paper in red biohazard bag.
Soak overnight in 1:10 bleach solution,
discard liquid in radioactive waste.
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Engineering Controls Check
for
Neurochemistry Core RIA Facility
Facility
Pharmacy Building #455 – Room P2-41
Location
Equipment
Inspection Dates
Action
Handwashing facilities
Monthly
Refill supplies
Sharps containers
Monthly
Empty if necessary
Specimen containers
Quarterly
Check for leaks
Regulated waste
Monthly
Check for leaks, empty if necessary
Waste containers
Monthly
Check for leaks, empty if necessary
Mechanical pipettes
Monthly
Check for contamination, clean if
necessary
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UF
Biomedical Waste Plan
For
Neurochemistry Core RIA Facility
Facility
Pharmacy Building #455 – Room P2-41
Location
This plan is based upon the UF Biological Waste Policy. It is designed to ensure
compliance with all local, state, and federal regulations and guidelines
concerning biological waste, including 29 CFR Part 1910.1030, the OSHA
Bloodborne Pathogen rule, and Chapter 64E-16, Florida Administrative Code,
Biomedical Waste rule.
A. Biomedical Waste
In this facility, the following items constitute biomedical waste:
Gloves, disposable garments, test tubes, pipettes, needles and syringes used in
preparation and analysis of blood or tissue samples from human or animal
sources.
B. Disposal Containers
1. Red plastic sharps containers shall be used for:
needles, syringes, razor blades, scalpel blades
2. Red biohazard bags shall be used for:
blood and tissue samples and any container or material in which is
contaminated by the blood or tissue samples.
3. Cardboard biohazard boxes shall be used for:
gloves, test tubes and pipette tips
4. Regular trash receptacle:
paper waste, non-radioactive.
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C. Personal Protective Equipment
The following personal protective equipment shall be worn when handling,
packaging and disposing of biomedical waste:
Gloves protective eyewear, lab coat (disposable and cloth), mask (as necessary)
D. Labeling
All packages containing biohazardous/biomedical waste shall be labeled with
indelible ink marker (i.e. Sharpie) as follows:
1. Biohazard bags:
Investigators Name
Phone Number
Room Location
2. Sharps containers:
Investigators Name
Phone Number
Room Location
3. Corrugated boxes (biomedical waste boxes):
Investigators Name
Phone Number
Room Location
E. Transport
Transport of biomedical waste shall take place as follows:
EH&S Waste Management Picks Materials Up and Properly Discards
Materials following OSHA Guidelines
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F. Training regarding biological waste shall take place as follows:
Each member of the Neurochemistry Laboratory will be required to undergo
annual training by Dr. Millard. This includes an update on all new OSHA and
EH&S mandates and viewing of any videotapes that are appropriate.
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