BIOL 2210 – Introductory Microbiology
Spring 2012
Dr. Heather Townsend
Lab Schedule
Lab
1
Date
1/23
2
1/30
3
2/6
4
2/13
5
2/20
6
2/27
7
3/5
8
9
3/1216
3/19
3/26
Exc. #
H
H
3.1 & H
3.3
1.2
1.3 & H
1.4
H
2.1
3.3
2.2
3.4
3.4
3.5
Topic
Introduction and safety
MSDS
Introduction to the Light Microscope
Examination of Eukaryotic Microbes
Nutrient Broth and Nutrient Agar Preparation (demo)
Common Aseptic Transfers and Inoculation Methods
Streak Plate Methods of Isolation
Pour Plate Methods of Isolation
Ubiquity of Microorganisms
Examination of Bacterial Slides (continued)
Colony Morphology
Smear Preparation
Simple Stain
Negative Stain
QUIZ # 1
3.6
3.7
3.9
2.6
2.7
6.1
Gram Stain
Acid Fast Stain
Endospore Stain
Fluid Thioglycolate Media
Anaerobic Jar (& Candle Method)
Standard Plate Count
2.8
2.10
2.11
Effect of Temperature on Microbial Growth
Effect of Osmotic Pressure
The Lethal Effect of Ultraviolet Radiation
QUIZ # 2
H
H
H
2.12
7.2
Effect of Bleach on Microbial Growth
Effect of Spices on Microbial Growth
Study of Oligodynamic Action on Bacteria
Chemical Germicides: Disinfectants and Antiseptics
Antibiotic Sensitivity Testing
Spring Break
8.3
Transformation of pARA-R plasmid in E. coli LMG
Unknown Bacteria I Tests:
1.4
3.6
3.9
3.7
5.14
2.6
5.22
5.17
2.8
Streak Plate Method of Isolation
Gram Stain
Endospore Stain
Acid Fast Stain
Gelatin Hydrolysis Test
FTM Tube
Motility Test
SIM Tube
Cardinal Temperatures
10
4/2
Unknown Bacteria I Continued (check last weeks results)
2.2
5.2
H
5.3
5.4
5.5
5.6
5.7
5.11
5.12
5.13
H
11
4/9
Colony Morphology
Phenol red broth (glucose)
Mannitol, sucrose and lactose
Methyl Red Voges-Proskauer tests
Catalase test
Oxidase test (TSA Plates)
Nitrate reduction test
Citrate test
Starch hydrolysis
Urea hydrolysis
Caesin hydrolysis test
Tryptone
QUIZ # 3
Unknown Bacteria I Continued (check last weeks results)
12
4/16
13
4/23
14
4/30
5.20
9.5
H
H
H
H
1.1
**H = Handout
Blood Agar Streak
Unknown Bacteria II – Enterotube
Examination of S. aureus
Bacteriological Examination of Water
Bacteriological Examination of Food
Bacteriological Examination of Soil
Glo-germ hand wash education
Review
FINAL Lab Practical