GENETICS
CORE FACILITIES
CATALOG
Cell Center Service Facility
DNA Sequencing Facility
Genetic Diagnostic Laboratory
Transgenic & Chimeric Mouse Facility
and the
Cell Center Stockroom
2004
UNIVERSITY OF PENNSYLVANIA
DEPARTMENT OF GENETICS
Haig H. Kazazian, Jr., M.D.
Chairman, Department of Genetics
Director, DNA Sequencing Facility
475 Clinical Research Building
215/898-3582
David Gasser, Ph.D.
Professor, Department of Genetics
Director, Cell Center Service Facility
575 Clinical Research Building
215/898-5175
gasserd@mail.med.upenn.edu
Tapan Ganguly, Ph.D.
Technical Director, DNA Sequencing Facility and Cell Center Service
Facility
D6 Richards Building
215/573-7238
gangulyt@mail.med.upenn.edu
Barbara Nevin
Manager, Cell Center Stockroom
D1 Richards Building
215/898-0281
nevin@mail.med.upenn.edu
- Please note that Barbara Nevin should be your first contact for issues regarding the Cell Center Stockroom.
- We appreciate your suggestions regarding ways in which we can increase the effectiveness of the Cell Center’s services. Your input,
addressed to either us or to your department or Center representative on the Advisory Committee, would be helpful and appreciated.
Thank you for your continued support.
2004 GENETICS
CORE FACILITIES
CATALOG
TABLE OF CONTENTS
DNA Sequencing Facility ............................................................................... 4
Description of Services ......................................................................................................
Charges for Services ..........................................................................................................
Recommended Procedures for Template Preparation .......................................................
Network Connections ........................................................................................................
Guide for Oligonucleotide Synthesis ................................................................................
5
7
8
12
13
Cell Center Services Facility.......................................................................... 17
Cell Center Services and Shared Resources ...................................................................... 17
Summary of Charges for Services ..................................................................................... 21
Genetic Diagnostic Laboratory ..................................................................... 22
Transgenic & Chimeric Animal Facility....................................................... 31
Cell Center Stockroom .................................................................................. 48
Opening an Account ........................................................................................................... 48
How to Order ...................................................................................................................... 49
Order Pickup and Delivery Information ............................................................................. 50
Oligonucleotide Synthesis .................................................................................................. 51
Primers & Probes ................................................................................................................ 54
Stocked Products - Product Class Listings .................................................. 57
Chemicals and Reagents • (CH).......................................................................................... 58
Competent Cells • (CE) ...................................................................................................... 57
Film and Membranes • (FM) .............................................................................................. 63
Immunological Reagents • (IM) ......................................................................................... 64
Miscellaneous Products • (MS) .......................................................................................... 68
Molecular Biology Kits • (KT) ........................................................................................... 65
Molecular Biology Products • (MB) .................................................................................. 66
Non-radioactive Kits and Reagents • (NR). ....................................................................... 69
Other Enzymes • (OE) ........................................................................................................ 69
PCR Reagents and Nucleotides • (PC) ............................................................................... 71
Precast Gels • (PG) ............................................................................................................ 74
Protein & Molecular Weight Markers • (MR) .................................................................... 67
Purification Kits and Columns • (CO)................................................................................ 62
Restriction Enzymes • (RE) ............................................................................................... 74
Sera • (SM) ........................................................................................................................ 78
Tissue Culture Media and Reagents • (TC) ....................................................................... 78
Stocked Products - Listed by Vendor ........................................................... 80
Amersham Biosciences • (AMR) .................…………….................................................. 80
Applied Biosystems • (PEC) ............................................................................................ 101
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 2
Bio-Rad Laboratories • (RAD) ........................................................................................ 106
Biowhittaker Molecular Applications • (BMA) ................................................................ 85
Cambrex Biosciences • (FMC) ......................................................................................... 86
Cell Center Services • (CCS) ............................................................................................. 86
Clontech Laboratories • (CLN) .......................................................................................... 87
Collaborative Biomedical Products • (COL) ..................................................................... 87
Denville Scientific • (DEN) ............................................................................................... 88
Difco Laboratories • (DIF) ................................................................................................. 89
Enzo Life Sceinces • (ENZ) ............................................................................................... 89
Epicentre Technologies • (EPI) ......................................................................................... 89
Fisher Scientific • (FSH) .................................................................................................... 89
Fuji Film • (FUJ) ................................................................................................................ 91
Gemini Bioproducts • (GEM) ............................................................................................ 91
Hyclone Laboratories • (HYC) .......................................................................................... 91
IGEN International (Fisher Scientific) • (IGN) ................................................................. 91
Invitrogen (Life Techologies) • (INV) ............................................................................... 91
Kodak • (KOD) .....................................................................................…......................... 97
Marligen Biosciences • (MLG)........................................................................................... 97
Mediatech (Fisher Scientific) • (MED) .............................................................................. 97
Micron Separations Inc. (Fisher Scientific) • (MSI) .......................................................... 98
Millipore • (MIL) ................................................................................................................ 98
National Diagnostics • (ND) ............................................................................................... 99
New England Biolabs • (NEB) ........................................................................................... 99
Perkin Elmer (New England Nuclear) • (NEN) ................................................................ 101
Polaroid Film • (POL) ....................................................................................................... 102
Promega • (PRO)....................................................................................…........................ 103
Q-Biogene • (DEN) ............................................................................................................ 88
Qiagen • (QIA) .................................................................................................................. 105
Rainin Instruments • (RAI) ............................................................................................... 107
Roche Diagnostics • (BMB) .........................………………………………………......... 82
Sigma-Aldrich • (SIG) ...................................................................................................... 107
Stratagene • (STR) ..................................................................................…...................... 108
TKR Bioproducts • (DEN) ................................................................................................ 88
USB Corporation • (USB) ............................................................................................... 109
Index of Stocked Products .......................................................................... 109
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 3
DNA SEQUENCING
FACILITY
Haig H. Kazazian, Jr., M.D., Director
215/898-3582
kazazian@mail.med.upenn.edu
Tapan Ganguly, Ph.D., Technical Director
215/573-7238
dnaseq@mail.med.upenn.edu, gangulyt@mail.med.upenn.edu
Peiqui Chen, Research Specialist
215/573-7407
pchen2@mail.med.upenn.edu
Latonia Clayton, Research Technician
215/573-7407
latonia@mail.med.upenn.edu
Deborah McKinney, Administrative Assistant
215/573-7407
dlmckinn@mail.med.upenn.edu
Elias Papaioannou, Research Specialist
215/573-7407
eliasp2@mail.med.upenn.edu
Lanping Zhang, Research Specialist
215/573-7407
lpzhang@mail.med.upenn.edu
David Zhu, Research Specialist
215/573-7407
zhu2@mail.med.upenn.edu
Location:
B1 Richards Building
Mailing Address:
B1 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104-6085
Telephone: 215/573-7407
Fax: 215/573-9327
Internet: http://www.med.upenn.edu/dnaseq
FTP: dnaseq.med.upenn.edu
Fast turnaround • Consultation and technical support • Automated cycle sequencing from PCR
products to BACs • Sequencing large fragments & project management • In-house primer &
oligonucleotide synthesis • Degenerate, inosine, phosphorothioate, biotinylated, amino linked, and
fluorescent labeled oligos • Template preparation and purification • GeneScanning for VNTRs •
Identification of heterozygosity • Assembly and analysis of sequences, ORF analysis • Genbank
homology searches • Submission of new sequences to Genbank
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 4
DNA Sequencing Facility
Description of Services
_ Please fill out a “Request for Services” form appropriate
to each service
_ Please acknowledge services performed by the facility
in your publications
New Service
We are now offering Site-directed Mutagenesis as a
service using Stratagene’s Quick Change kit. This includes
design and synthesis of the mutagenic primers, mutagenesis
and verification of the mutation by sequencing.
Synthesis and PAGE purification of the primers are done by
LTI (BRL). Please contact us for details.
Also, we are now in the process of setting up
Transgenomic’s DHPLC system for mutation detection,
DNA fragment separation, and oligonucleotide purification.
Automated Cycle Sequencing
The facility uses ABI 377 and 373A Stretch sequencers,
with Taq FS Big Dye™ Terminator or Dye Primer chemistry.
Please consult our recommendations for template preparation
- to avoid problems. Standard primers (listed below)
are provided by the facility at no charge. Custom primers
should be provided by the user, or can be synthesized at
the facility. Facility users can choose between short (~500
nucl accurate read) or long (~600 nucl accurate read) gels.
Ready-to-load samples are analyzed at a lower charge.
Turn around time is generally 2 working days for sequencing.
Color printouts of analyzed chromatograms are
provided for all successful reactions. Sequencing results
can also be obtained on floppy or Zip disc, or over
PennNet, or by FTP for further examination and manipulation
by the user. Facility staff will consult with users
whenever there are problems, and will make suggestions
to improve sequencing results. A good template yields 600
nucleotides of reliable sequence (<2% error and ambiguity)
in a long read run.
Templates or template-primer mixes for each reaction must
be in a separate 1.5 ml tube.
Our ability to provide fast turnaround and reliable sequencing
service depends on you! Please provide the correct
amounts and volumes of templates and primers (as
indicated on the Sequencing Request Form), and make
sure all the requested information on the form is completed.
The following standard primers provided by the Sequencing
Facility at no charge are listed in Table 1.
Template Preparation and Purification
Template DNA can be prepared and/or purified by the
facility staff. Low cost plasmid minipreps processed on the
QIAGEN BioRobot are offered with or without sequencing.
Larger scale purification is also available. Lambda phage
cosmid, PI and BAC preps of comparable scales are also
performed. PCR reactions and purification using commercial
columns, with or without gel analysis, is also offered by
the facility.
The facility also offers a reliable treatment of marginal or
bad templates to make them suitable for automated
sequencing. This involves RNase treatment followed by
column purification or phenol-chloroform extraction. This
treatment improves the quality of the template, producing
more reliable sequence and longer reads. For primer
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 5
walking projects we recommend that a substantial amount
of DNA (nominally 4-5 µg per kb for each direction) be
treated this way and be used for the whole project.
Templates prepared or purified by the facility staff are
guaranteed to produce good sequence with standard,
reliable primers. The facility also provides free training in
template preparation and troubleshooting at the user’s
request.
Oligonucleotide Synthesis
Two ABI 394 four-column synthesizers are used for
synthesizing primers for sequencing projects and oligos
requested by researchers. After synthesis, oligonucleotides
are unprotected, yield is measured by OD260, and
the oligo is desalted (if necessary) and dried. Affinity
column OPC purification of full-length oligos is available if
requested. Each column can be loaded with 5-15 OD260
of oligo, and will yield 3-8 OD260 after purification. DegenTABLE 1: Standard Primers Available at No Charge
Primer Sequence Bases M.W. TmoC
M13/pUC Forward 5’ CCCAG TCACG ACGTT GTAAA ACG 3’ 23 6978 69.2
M13/pUC Reverse 5’ AGCGG ATAAC AATTT CACAC AGG 3’ 23 7066 65.8
Sp6 5’ ATTTA GGTGA CACTA TAGAA 3’ 20 6164 47.6
T3 promoter/primer 5’ ATTAA CCCTC ACTAA AGGGA 3’ 20 6094 56.7
T7 promoter/primer 5’ TAATA CGACT CACTA TAGGG 3’ 20 6125 50.8
T7 terminator 5’ GCTAG TTATT GCTCA GCGG 3’ 19 5830 60.1
gt 10 Forward 5’ CCTTT TGAGC AAGTT CAGCC TGG 3’ 23 7025 69.7
gt 10 Reverse 5’ GGTGG CTTAT GAGTA TTTCT TCC 3’ 23 7046 61.5
gt 11 Forward 5’ ATTGG TGGCG ACGAC TCCTG GAG 3’ 23 7112 74.2
gt 11 Reverse 5’ CAGAC CAACT GGTAA TGGTA GC 3’ 22 6763 62.2
DNA SEQUENCING FACILITY
7
erate, inosine containing, phosphorothioate, biotinylated,
5’or 3’ phosphorylated oligos, and oligos with terminal
amino groups or fluorescent tags can be synthesized.
Turnaround time is typically 2-3 days.
Large Sequencing Projects
The facility will sequence through DNA clones by primer
walking and/or subcloning. Please fill out a Request for
Sequencing Project Management form when submitting the
sample, indicating if you wish to select primer sequences
yourself or delegate the responsibility to facility staff.
Charges for primer walks are calculated according to the
number of lanes sequenced and primers synthesized. A
clean-up treatment of sufficient template for the whole
project (nominally 4-5 µg per kb for each direction) is
recommended to produce quality sequence and maximize
step size. After good quality template is obtained, each
step (~450 nucl.) requires about a week, including primer
TABLE 2: Charges for Services
Cancer Center Other
& Genetics Members Investigators
DNA Sequencing
Long Run
DNA Sequencing (reaction, purification, electrophoresis) .............................. $17.50/sample $19.50/sample
DNA Sequencing Reaction Done (purification, electrophoresis) .................... $12.50/sample $14/sample
DNA Sequencing Ready to Load (electrophoresis of sample only) ....................... $9/sample $10/sample
Short Run
DNA Sequencing (reaction, purification, electrophoresis) .............................. $14.50/sample $16/sample
DNA Sequencing Reaction Done (purification, electrophoresis) ......................... $10/sample $11/sample
DNA Sequencing Ready to Load (electrophoresis of sample only) ....................... $7/sample $8/sample
Template Preparation/Processing
Template cleanup with gel and spec analysis ..................................................... $27/sample $30/sample
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 6
Plasmid Miniprep (1-5 samples) ....................................................................... $5.40/sample $6/sample
Plasmid Miniprep (5 samples or more) ............................................................. $3.60/sample $4/sample
Phage Miniprep ................................................................................................... $27/sample $30/sample
Plasmid or phage Midiprep (1-2 samples) ........................................................... $30/sample $33/sample
Plasmid or phage Midiprep (3 or more samples) ........................................... $20.50/sample $23/sample
Plasmid or phage Maxiprep (1-2 samples) .......................................................... $40/sample $45/sample
Plasmid or phage Maxiprep (3 or more samples) ............................................... $30/sample $34/sample
Plasmid or phage Mega prep (1-2 samples) ....................................................... $72/sample $80/sample
Plasmid or phage Mega prep (3 or more samples) ............................................. $57/sample $64/sample
PCR amplification of template (1-2 samples) ...................................................... $11/sample $12/sample
PCR amplification of template (3 or more samples) ......................................... $6.50/sample $7.50/sample
PCR product purification (column) ...................................................................... $13/sample $15/sample
PCR product purification (gel) .....................................................................................$22.50 $25.00
Transformation ............................................................................................................ $22.50 $25.00
Site-directed Mutation, Mutagenic Oligos not included .............................................$200.00 $225.00
Sub-cloning, DNA miniprep and Sequencing not included ........................................ $117.00 $130.00
GeneScanning / Fragment Sizing
GeneScan with lane tracking and size calling (up to 48 lanes) ................................ $135/gel $150/gel
GeneScan without tracking/sizing (up to 48 lanes) .................................................... $90/gel $100/gel
Oligonucleotide Synthesis and Purification
Note: There is no Setup Charge for Oligo Synthesis
Oligonucleotide synthesis 40 nmole (0.3-0.5 OD/base) ......................................... $.85/base $.90/base
Oligonucleotide synthesis 0.2 µmole (1-2 OD/base) ............................................ $1.25/base $1.40/base
Oligonucleotide synthesis 1.0 µmole (5-8 OD/base) ............................................ $3.15/base $3.50/base
OPC purification (5-10 OD units per column) ...................................................... $11/column $12/column
Oligo w/ 5’ aminolink ............................................................................... One additional base
Oligo w/ 3’ aminolink ................................................................................................. Add $20
Oligo w/ 5’ biotin ....................................................................................................... Add $15
Oligo w/ inosines ...................................................................................... Add $2 per inosine
Oligo w/ 5’ fluorescent tag (FAM, HEX, TET) ........................................................... Add $20
Oligo w/ phosphorothioate bonds .......................................................... No additional charge
Oligo w/ phosphorylation (3’ or 5’) .......................................................... One additional base
Analysis/Assembly
Training is available at no charge.
selection and synthesis, sequencing, sequence evaluation,
and assembly. Charges for subcloning should be discussed
with facility staff. Assembly of the overall sequence,
ORF analysis, and homology searches can be
performed, at no charge, if requested by the user.
GeneScan
Identification of VNTR alleles and other sizing application
can be performed on the 377 using GeneScan gels and
software. Reduced charges apply if the user performs lane
tracking and size calling. Charges are per gel and each gel
can be loaded with 48 multiplexed samples.
Sequence Assembly and analysis
Assembly of sequences into a contig, identification of
ORFs, analysis of properties of the predicted protein, and
other analyses can be performed at the facility using
MacVector and other software. These services are
included at no charge for large sequencing projects, and
they are available at no charge to Cancer Center and
Department of Genetics members.
Database Searches
BLAST, BLOCKS, BLITZ, PROSITE and FASTA searches
are performed using WWW or e-mail servers at no charge.
Please contact the facility to arrange for database searches
or for training. Genomic sequences can also be processed
to identify interespersed repeats, low complexity regions,
GRAIL exons, transcription factor binding sites, frequency
of mono, di, and tri nucleotides, and other features.
Genomic sequences can also be submitted to the GAIA
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 7
genome annotation system at the Bioinformatics
Recommended Procedures for Template
Preparation
Evaluating your DNA template
PLEASE NOTE: Criteria for cycle sequencing are more
stringent than manual sequencing and many other molecular
biological procedures. The success of sequencing
reactions depends critically on having high purity template
at the correct amount. The most important contaminants
are bacterial proteins and cell wall carbohydrates, and
organic solvents (ethanol, isopropanol, etc.). Please follow
these recommendations for checking concentration and
purity of your template DNA:
• Scan DNA sample in a spectrophotometer between
220 and 320 nm. If you do not have access to a
scanning spec, measure absorbance at 220, 260 and
280 nm. In Figure 1, the solid line shows the absorption
profile of pure DNA (RNA profile is very similar),
and the dashed lines indicate how this profile is
modified by the presence of contaminants. Nucleic
acids have their peak absorbance at 260, with a 260/
280 ratio of 1.8 for DNA, 2.0 for RNA. Estimate DNA
concentration from OD260: conc (ug/ml) = OD260 X
dilution factor X 50. Remember that OD260 can have
contributions from DNA as well as contaminants.
• Run a sample of DNA on an agarose gel with a
quantitative standard (e.g. lambda HindIII digest,
where the amount of DNA in each band is known). By
comparing intensities of template band with comparable
size bands in standard lane, estimate concentration
of DNA (see Figure 2). Compare this estimate to
the OD260 estimate of DNA concentration. If OD260
estimate is significantly higher than gel estimate, it
indicates contamination with RNA or RNA fragments.
An RNase treatment, followed by phenol/chloroform
extraction and precipitation would be recommended.
• If the scan profile or low OD260/280 ratios indicate
protein/carbohydrate contamination, perform the
phenol extraction and precipitation procedure described
for optional cleanup below. If organic contamination
is suspected (OD220>>OD260), you can
usually remove them by precipitation and redissolving.
Alternatively, small contaminants can be washed away
by centrifugation through a microcon or centricon
ultrafiltration concentrator.
Plasmids
Grow 1 colony in 5-25ml LB + antibiotic overnight (~16hrs)
at 37oC with shaking. Cell density should not exceed 1.5
A600 units. Debris from high-density culture inhibits the
reaction.
A. Purification by QIAGEN Columns
Use 1.5 ml of culture lysate per mini column, 5-10 ml
per midi column, or 50-100 ml per maxi column. Follow
QIAGEN procedure, but add fresh RNase to 10U/ml
(instead of, or in addition to, the RNase in the P1
buffer). Mix suspension very gently during and after
cell lysis. Centrifuge lysate twice after lysis (supernatant
should be clear). Treat with an additional 10U/ml
RNase for 30min at 37oC before loading onto the
column. Following isopropanol precipitation, redissolve
DNA in water and precipitate with ethanol: add 1/10
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 8
volume 3M sodium acetate + 3 volumes absolute
ethanol, chill on ice 30 minutes. Spin at 10,000 RPM
for 20 min, 70% ethanol wash, dry, redissolve in water
(salts inhibit sequencing reaction).
B. Purification by Promega Wizard™ Mini Preps
Use 3ml of culture per mini prep and follow Promega
procedure. Spin lysate twice. Be sure the supernatant
is clear before adding resin. It is often necessary to
concentrate eluted samples by ethanol precipitation or
drying. Precipitation also improves quality of template
FIGURE 1 Spectroscopic analysis of DNA sample
and allows resuspension in water.
C. Optional Purification
1. RNase treatment, followed by phenol/chloroform
extraction and precipitation improves template
quality, but care must be taken to remove all
organics. Most samples treated in this manner
can be sequenced successfully. Incubate with 10
U/ml fresh RNase for 30 min at 37oC, extract with
phenol (until interphase is clear), phenol/chloroform,
then chloroform. Precipitate with ethanol,
wash pellet with 70% ethanol, dry, and redissolve
in water.
2. Template quality may also be improved using a
100K centrifugal concentrator (minicon, centricon
– dilute 5-10 µg of DNA to 50-100 µl with water
and centrifuge through concentrator at 3000g for
10 min, add water and repeat 3-5X) or by dialysis
for 2hr using an 100K MW cut off Millipore
Minitan-S membrane (float the dry membrane
shiny side down on sterile water and apply the 20
µl DNA sample).
3. PEG precipitation is also helpful in improving
quality of template. Add 0.25 vol 4M NaCl and
1.25 vol 13% PEG 8000, incubate on ice 20 min,
pellet DNA, redissolve in water.
D. Alternative methods
Templates purified by phenol-chloroform extraction or
CsCl gradients can be used, as long as sufficient
further precipitations/washes are done to remove
organics/CsCl.
PCR Products
If a PCR product produces a single band, purify with
QIAGEN or Wizard™ PCR purification columns. These
columns use salts and ethanol, so extensive washing of
pellet and complete drying of ethanol is necessary. Several
washes with water through a microcon 100 concentrator
can also be used for purification or clean-up. If there are
multiple bands, run products on low melting point agarose
gel, excise band, purify DNA using QIAGEN QIAEX II or
Promega Wizard™ PCR preps following recommended
procedure. Alternatively, run products on a gel, cut and
electroelute desired band. Quantitate template on gel
(OD260 can be misleading if salts/alcohols are left in
sample). Submit 10ng of DNA / 100 bp / reaction in = 5 µl
water.
Bacteriophage Lambda
Lambda preps isolated by two consecutive bandings in
CsCl, followed by RNase treatment and phenol-chloroform
extraction, yield good DNA for sequencing. Purification by
QIAGEN Lambda prep columns also gives adequate
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 9
results. Below is a procedure for preparing lambda DNA by
extractions and precipitations.
1. Place 200 µl of a healthy overnight culture of the
appropriate host strain into a clear plastic, sterile 10 ml
snap-cap tube. The choice of bacterial strain is
important. Choose a host in which lambda replicates
vigorously, e.g. use strain Y1090 for a lambda gt11
clone. If appropriate, include 0.2% maltose in culture
medium to induce the lamB gene that codes for lamba
receptors.
2. Stab a well-separated plaque with a sterile toothpick
and rinse the captured lambda phage particles into the
cells aliquoted above. Alternatively, use 3X10**6 pfu
of phage from a stock. Allow the phage to adsorb to
the cells for 15 minutes at room temperature.
3. Add 2 ml Tryptone Broth containing 10 mM MgSO4
and shake the culture at 37oC until the culture is lysed.
Lysis is evident when the culture becomes clear and
cellular debris is prevalent. This will take anywhere
from 4 to 8 hours depending on both the host and
lambda strain used. Add 10-100 µl chloroform and
continue shaking to complete lysis if necessary. Pellet
cellular debris at 10,000 rpm for 5 min.
4. Pour the supernatant into a new 2 ml tube. Add 10 µl
2000U/ml RNase A and 5 µl 10 mg/ml DNase I.
Incubate the sample at 37oC for 20 minutes.
5. Add 0.12 g NaCl and invert the tube several times until
the salt is dissolved, then add 0.2 g polyethylene
glycol (8000 average MW) and invert the tube repeatedly
until the PEG is dissolved. Let the sample sit at
4oC for 1 hr to overnight.
6. Spin in a microcentrifuge for 20 minutes. Resuspend
pellet in 0.5 ml SM (50 mM Tris, pH 8.0, 100 mM NaCl,
10 mM MgS04) and transfer to a 1.5 ml tube.
7. Extract the sample once with chloroform. To the
aqueous layer, transferred to a new tube, add: 40 µl
250 mM EDTA pH 8.0, 5 µl 20% sodium dodecyl
sulfate, 2.5 µl of 10 mg/ml Proteinase K, and incubate
the sample at 60oC for 30 minutes.
8. Extract twice with phenol/chloroform, then twice with
chloroform. Add 40 µl 3 M sodium acetate, pH 5.5 and
FIGURE 2: Gel analysis of DNA sample
In this example, 1 ul of lambda HindIII digest
containing 500 ng DNA was loaded in the outer
lanes, and 10 ul of sample was loaded in lanes 2
and 3. Since the lambda genome is 50 kb in size,
the 4.4 kb band contains 500 X 4.4/50 = 44 ng of
DNA. The sample in lane 2 is a little brighter,
probably containing 80 ng of DNA. Therefore, DNA
concentration in the sample is 8 ng/ul.
1ml 95% ethanol. Spin at room temperature for 10
minutes.
9. Rinse the pellet with 750 µl cold 70% ethanol. Resuspend
the DNA pellet in 400 µl distilled water.
10. Add 200 µl 40% PEG8OOO, 30 mM MgSO4. Mix
thoroughly, let stand at room temp for 20 min.
11. Extract twice with chloroform. Add 40 µl 3 M sodium
acetate and 1 ml 95% ethanol. Mix thoroughly. Spin at
room temp for 10 min.
12. Rinse the pellet with 750 µl cold 70% ethanol. Air dry
pellet and resuspend in 50 µl distilled water. Yield is 12 µg.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 10
BAC DNA purification
Recently QIAGEN and Princeton Separations have
introduced their BAC DNA kits. We have mixed results with
both.
We describe here the NH4Ac/QIAGEN midi prep method.
BAC DNA prepared by CsCl gradient centrifugation,
followed by Rnase treatment, extraction and precipitation
work well as templates. Below is a modified alkaline lysis
method that yields good quality DNA.
1. Grow 200 ml of culture. OD 600 should be between 1
and 1.5.
2. Spin down cells and resuspend in 15 ml of P1 adding
fresh RNase (150 units).
3. Add 15 ml of P2, mix by gentle inversion and incubate
for 5 minutes at room temperature or until lysis looks
complete.
4. Add 15 ml of P3 and keep on ice for 15 minutes. Mix
every 3 minutes by gentle inversion.
5. Spin down cell debris 13,000 rpm for 30 minutes at 4
C. Spin a second time for 15 minutes to get rid of any
debris still in suspension.
6. Transfer supernatant to a fresh tube and precipitate
with isopropanol. Wash pellet with 70% ethanol.
7. Resuspend pellet in 1 ml H2O and add 0.25 ml 10 M
NH4Ac to a final concentration of 2 M NH4Ac. Pellet
precipitate by centrifugation at 13,000 rpm 30 min,
keep supernatant.
8. Transfer supernatant to a new tube and precipitate
DNA with 2 volumes of ethanol. Pellet at 10,000 rpm
for 15 minutes.
9. Redissolve pellet in 2 ml of H2O and add 10 ml of
QBT.
10. Equilibrate QIAGEN tip 100 with 4 ml of QBT. Apply
sample to column.
11. Wash column with 10 ml of QC three times. Elute
DNA with 4 ml of QF.
12. Divide eluted DNA into 6 microfuge tubes, 0.67 ml
each. Add 0.670 ml of isopropanol and chill on ice for
15 min.
13. Spin DNA down at 10,000 rpm for 15 min and wash
pellet with 70% ethanol as before.
14. Dissolve in water after air drying. Yield is 5-8 µg.
Dye Terminator Cycle Sequencing
Generally follow protocol given by ABI for Taq FS and Big
Dye™ terminator chemistry. Consult ABI protocols or
facility staff for other chemistries. Typically, template is in 6
µl volume, primer in 3 µl, and reaction mix with Taq
polymerase in 4 µl. Add DMSO to 3%, and double Taq
concentration, for GC rich or difficult templates. Total
reaction volume is 13 µl. Table 3 shows template, primer
and reaction condition requirements for different templates
when using Big Dye™ terminators and Taq FS:
Reaction products are purifed with AGTC 96 Well Gel
Filtration Block (Edge Biosystems). Purification can also be
done with Centrisep Spin columns (Princeton Separations)
or a Sephadex G-50 Quick Spin column from BoehringerMannheim, or Centrisep column washed and repacked with
G-50.
AGTC 96 Well Gel Filtration Block
Variable speed centrifuge equipped with a rotor and a
microplate carrier is required. Follow manufacturer’s
instructions.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 11
CentriSep
Swell dry gel with 0.75 ml of water for 1hour. Spin in fixed
angle microfuge rotor at 750g (~3,000 rpm) for 2 min. Put
tubes in with mark oriented up, so that you can perform
second spin in same orientation. Load sample in center of
gel, repeat spin with tube in same orientation as the first
spin.
BMB Spin Columns
If using an angle rotor, orient the columns the same way for
both spins. After the first spin, the gel material within the
column will pull away from the column walls. Be sure to
load your sample in the center of the gel material.
Dry down sample in SpeedVac. Resuspend in 2.0 µl of
loading buffer, denature for 2 min at 95 °C (DO NOT
OVERHEAT!), keep on ice, load.
Loading buffer: 5 parts deionized formamide + 1 part 25
mM EDTA, 6% blue dextran, pH 8.0
TABLE 3: Amounts of template and primer to be submitted (Please add primer to your template in the suggested volumes)
Template type Size (kb) Template/rxn Suggested concXvol Primer amount Primer conc.
PCR product 0.1-1 10 ng per 100 bp 1.6-16 ng/µl X 6µl 3.2 pmoles 1.1 µM X 3µl
Plasmid 2-15 0.5 µg 80 ng/µl X 6µl 3.2 pmoles 1.1 µM X 3µl
Phage/cosmid/P1 20-100 1.0 µg 110 ng/µl X 9µl 12 pmoles 4 µM X 3µl
BAC 200 2.0 µg 220 ng/µl X 9µl 12 pmoles 4 µM X 3µl
Network Connections
A. You can communicate with the sequencing facility,
receive information, and order oligos by e-mail:
Tapan Ganguly: ....................dnaseq@mail.med.upenn.edu
Debi McKinney .................. dlmckinn@mail.med.upenn.edu
Peiqui Chen .........................pchen2@mail.med.upenn.edu
Elias Papaioannou ............... eliasp2@mail.med.upenn.edu
Lanping Zhang .................... lpzhang@mail.med.upenn.edu
David Zhu .................................zhu2@mail.med.upenn.edu
B. Access for all Macintosh users:
Macintosh computers running System 7.6 or later can use
AppleShare to connect to our server over the Internet,
even if they are not connected directly to PennNet.
(You may need to update your AppleShare software to do
this, and we urge you to contact your Local Support
Provider for more information. Updates can be downloaded
from http://asu.info.apple.com/swupdates.nsf/artnum/
n11419.)
You will need to contact the facility and choose a user/
folder name and a password for your folder. We will put
your results in your folder to which only you have access.
Because of the large number of users and various users
with the same investigator, you must indicate on each
Sequencing Request Form that you want results put into
a folder, and give the folder name.
Results will be kept in your folder for up to one month.
All results are also archived by the facility if you need them.
Note that the purpose of the server is to enable you to copy
files and disconnect when done. Please do not doubleclick
the files on the server!
1. Select “Chooser” from the Apple menu.
2. Click on the AppleShare icon in the upper-left corner,
then click on the “Server IP Address…” button near the
lower-right corner.
3. In the input box which appears, type in
“dnaseq.med.upenn.edu” and click “Connect”.
4. When prompted, type in your lab folder name and
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 12
password and click “OK” or “Connect”.
5. In the next box that appears, “Sequencing Results”
should already be selected. Do not click the checkbox
on that top line, as this may slow down the
server’s performance and/or cause your computer to
crash. Just click “OK”.
6. Read the current update message that appears and
click “OK”.
7. Close the Chooser window, and you should find that
the “Sequencing Results” server volume is now on
your desktop.
On future occasions, you can skip the above steps by
selecting “Sequencing Results” from the “Recent Servers”
sub-menu under the Apple menu.
Your results will be located in your lab folder on the
“Sequencing Results” server volume. Please note that
results will be available after 11:00 a.m. on most days.
Copy the files to your computer, then disconnect from the
server by dragging the “Sequencing Results” icon to the
Trash.
Near the top of the folder list, you will also find installers for
Sequence Editor and EditView software for examining
sequence data, as well as Sequencher and ClustalW
software for multiple alignment of sequences. Be sure to
copy these items to your own hard drive rather than
installing the software directly from the server.
C. Other Internet Access:
Anyone with access to the Internet can copy their results
by FTP, using Fetch for Macintosh, WS-FTP for Windows
or other file transfer software. To do this, set up a user
name and password, as in section B above.
Connect to the FTP site “dnaseq.med.upenn.edu” using
your lab folder name and password. If your software has a
space for specifying the directory, leave it blank. Once
connected, find your lab folder in the directory listing and
open it. Select the files you want and transfer them to your
computer. Please disconnect from the server when finished
copying.
Guide for Oligonucleotide Synthesis
Introduction
Oligonucleotides are synthesized on the ABI 394 Synthesizer,
using solid-phase synthesis and phosphoramidite
nucleoside chemistry. Synthesis proceeds from the 3’ to
the 5’ end. The 3’ nucleoside is already coupled to the
solid support (polystyrene or glass), and activated nucleotides
are sequentially added on to this nucleoside. Bases
and phosphate groups are kept protected until the end of
synthesis. At each step, the sugar protective dimethyl
trityl (DMT) group on the deoxyribose is removed to allow
reaction with the next amidite. Since these reactions are
only ~99.5% efficient, a capping reagent is used to cap
unreacted sugars. This cap terminates synthesis on the
small proportion of oligos in each step that did not receive
the correct next base. Because of this cap, syntheses will
usually produce some terminated chain oligos, but no
significant amounts of oligos with incorrect (e.g., missing
internal base) sequence. Thus, the mixture of oligos will all
start with the same 3’ end, but some proportion of the
chains will be terminated early.
TABLE 4: Yields of oligonucleotides >(20-mer) at different scales
OD260 Yield (mg) Yield (nmoles) Conc. (suspended in 1 ml)
40 nmole scale 5-8 0.25-0.4 40-65 40-65 µM
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 13
0.2 µmole scale 20-40 1.0-2.0 160-320 160-320 µM
Yield & Efficiency
Printouts provided with oligos give two numbers that allow
you to evaluate the success of synthesis: the Average
StepWise Yield (ASWY), which give the average efficiency
of incorporation of nucleotides at each step, and the
Overall Yield, which represents the percentage of total
chains that are full-length. Typical values of ASWY and
overall yield in a successful synthesis of a 20-mer are
98.5% and 70%, respectively. Lower overall yields of fulllength
product are obtained with longer sequences.
Average yields per base for 40 nmole and 0.2 µmole scales
are 0.3-0.4 OD260 and 1.0-2.0 OD260 units, respectively.
The values for an average 20-mer (MW ~ 6,000) suspended
in 1 ml are listed in Table 4.
Purification
A typical synthesis generates a mix of full-length oligos
(which have the DMT group at their 5’ ends), and shorter
oligos (which are capped). Oligos synthesized in this
manner can be used for PCR and sequencing without OPC
purification because they have a well-defined 3’ ends (the
site of elongation by polymerases). Most PCR and
sequencing primers therefore do not require OPC
Purification. If the oligo is to be used as a linker or
adaptor and the integrity of the 5’ end is also essential,
then we recommend that you choose “OPC Purify”. An
affinity column (OPC) that recognizes the DMT group is
used to purify full-length oligos from the mix. Each OPC
column can be loaded with 5-10 OD of crude oligonucleotides
(which corresponds to the yield of a typical 40 nmole
synthesis), and will yield 2-5 OD of purified product. The
DMT group is removed during purification. For purification
of larger scale syntheses, please indicate if you want
purification of 10 OD only (one column), or all of the
produced oligo (multiple columns). Please note that
efficiency of full-length oligo production decreases with
length of oligo, and 0.2 µmole scale synthesis would be
recommended for long (>40) oligos that need to be OPC
purified.
Modifications
Oligos phosphorylated at either 3’ or 5’ ends can be
synthesized using the ABI phosphalink reagent. We can
also add a 3’ or 5’ amino group, 5’ biotin, or a 5’ fluorescent
dye (FAM, HEX or TET). Furthermore, phosphorothioate
oligos can be synthesized for antisense or other applications.
These modifications should be indicated on the
request sheet or discussed with the staff. Please note that
5’ phosphorylated oligos do not have a DMT group and
cannot be OPC purified.
Evaluating and Editing Sequence Results
Please do not start to work with the sequence text file
before you examine the chromatogram.
Sequence calls by the analysis software can have several
types of problems, and we recommend that all users
evaluate and edit the automated sequence calls as
necessary by examining the actual colored peaks in the
chromatograms. The areas that you should pay particular
attention to are:
1. Errors in base calls at the start of the sequence due to
noise. You may decide to trim away the initial bases
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 14
(10-20 bases).
2. Occasional bases missed in the first 50 bases due to
compression.
3. Possible miscalled bases in areas where signal may
be low.
4. Incorrect base calls beyond 600 bases where resolution
is deteriorating.
5. Determining where usable sequence stops, and
trimming the remaining base calls.
Three programs are available for viewing and editing
chromatograms: EditView, SeqEd, and Sequencher. You
can copy EditView and SeqEd from the Server, or download
them by clicking here. Sequencher is a commercial
software by GeneCodes, but the facility maintains network
sharable copies.
EditView allow opening, editing, exporting, and printing of
one sequence file at a time. It is self explanatory, and easy
to use.
FIGURE 3: A schematic diagram of solid phase
oligodeoxynulceotide
SeqEd allows opening multiple chromatograms if comparison
between them is desired. SeqEd is not a good assembly
program and should not be used for sequence assembly.
Instructions for using SeqEd are given below.
Sequencher is a sophisticated, commercial software for
sequence editing and assembly. It would require significant
effort to learn. If you are interested in using Sequencher,
ask the facility staff for a copy of the software. In addition,
copy the KeyServer extension from the Sequencher folder
on the server into your system folder, and follow the
instructions for how to run Sequencher using the network
KeyServer. Sequencher has an on-line help function that
explains the major features of the program.
Short Instructions for SeqEd
SeqEd allows you to examine chromatograms and the
associated sequence text file, correct errors, and extract
the reliable portion of the sequence from your results. We
strongly recommend that users of DNA sequencing
services review the chromatograms and edit the base calls
made by the ABI analysis software as needed. The
software sometimes makes obvious errors, particularly at
the beginning and near the end of a run. SeqEd also
allows you to open two or more chromatograms at the
same time for purposes of comparison. To use SeqEd,
follow these steps:
1. Double click on the program file SeqEd v1.0 to start it.
2. From the bar menu, select Sequences and then select
Import (or use the shortcut Apple-M). A window will
open up to allow you to choose the appropriate folder
and chromatogram file. Do not try to open a “.seq”
sequence text file. Select the chromatogram file and
open it. At this time only the sequence text will show.
Note that only the text part of the file is visible at this
time.
3. Repeat step 2 above to select additional chromatogram
files if desired. More than three chromatograms
per screen tends to be too crowded.
4. Select the SeqEd window that contain all the text
information of the files you imported, then select the
individual file names you want to visualize. You can
use shift-click to select multiple files.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 15
5. From the Sequences menu, select Display Chromatogram
(or use the shortcut Apple-D). All selected
chromatograms will appear on the screen in compressed
mode. If necessary, select specific files and
then select Reverse Complement Sequence from the
Sequences menu.
6. Select each chromatogram one at a time, click on the
“C” button in the control panel on the bottom left, then
click the pointer in an area near the beginning of the
chromatogram. The chromatogram will expand to
make peaks and the sequence visible.
7. Scroll along the chromatogram, looking for areas
where the base calls by the analysis software might be
in question. Look for missing bases, extra bases,
peaks that were called as N’s when they should not
have been (signal-to-noise > 2), or peaks that were
called as a base but should be N (signal-to-noise
<1.5). Click on the base or position you want to edit,
and type in the new base.
8. Decide where you think the reliable sequence ends.
Use both signal-to-noise and peak broadening to
determine the last reliable base. Click on it to determine
what base number it is.
9. Click on the top window containing the text sequence,
select the specific sequence you have just edited (if
working with multiple sequences), find the location of
the last reliable base, and delete the remainder of the
sequence.
10. From the Sequence menu, select Export (or use the
shortcut Apple-E). A window will appear to allow you to
choose the location of the file, its name, and what type
it is. Select Text, and change the name of the file (you
are not allowed to overwrite an original sequencing
result file). The edited text sequence will be stored in
the selected file and location.
11. This text file can be opened in word processors or
other sequence analysis software for further manipulation
and analysis.
12. When finished, quit SeqEd and answer No to all the
questions it asks before closing.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 16
CELL CENTER
SERVICES
FACILITY
David Gasser, Ph.D., Director
215/898-5175
gasserd@mail.med.upenn.edu
Tapan Ganguly, Ph.D., Technical Director
215/573-7238
gangulyt@mail.med.upenn.edu
Sabine Baxter, Research Specialist
215/898-2795
baxters@mail.med.upenn.edu
Diane Miller, Research Technician
215/898-2795
dianemil@mail.med.upenn.edu
Location:
D2 Richards Building
Mailing Address:
D2 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104-6085
Telephone: 215/898-2795
Fax: 215/573-9327
Internet: http://www.med.upenn.edu/ccsf/
Consultation • Training • Cell bank and Seed Cultures • Liquid Nitrogen Freezer Storage • Lymphocyte
& DNA Banking • EBV Transformations • Large Scale Cell Culture • Mycoplasma, Endotoxin and
MHV Testing • Specialty Media • Autoclaving • Hybridoma Production and Screening • Antibody
Production (supe or ascites), Purification & Conjugation • Cancer Center Shared Resources and
Facilities
U N I V E R S I T Y O F P E N N S Y LVA N I A
D E PA R T M E N T O F G E N E T I C S
Cell Center Services
_ Please fill out a “Request for Service” form when
using Cell Center Services
_ Please acknowledge services performed by the
facility in your publications
1. Consultation
The Technical Director will discuss projects with
investigators to explore and evaluate different experimental
designs and approaches utilizing cell culture
and immunological techniques. Establishment and use
of cell lines, strategies for production of monoclonal
antibodies, and other options can be explored with
investigators.
2. Personnel Training
The Cell Center will train laboratory personnel in basic
sterile and tissue culture techniques, hybridoma and
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 17
monoclonal antibody production procedures, and
immunological assays. An individual training program
in basic cell culture skills, which runs for a total of 12
hrs over a two week period, is offered for a charge of
$300. This program will expose inexperienced
personnel to basic techniques of cell culture, including
making media, thawing cells, cell count and determination
of viability, culture and subculture of attached cells
and cells in suspension, and freezing cells. We also
have available two 35 min videos and some
worksheets on cell culture techniques. These can be
signed out by users for 2-3 days at no charge. They
are useful for understanding basic sterile culture
techniques, or as a refresher for more experienced
people. Please contact us to arrange for training in
more specialized areas.
3. Seed Cultures (established lines from our cell bank)
1. T-25 ......................... $30.00
2. T-75 ......................... $35.00
3. T-150 ....................... $40.00
We can also order ATCC cell lines not available in our
cell bank (usual cost ~$120).
4. Preparation of Special Media
The Cell Center prepares and sterilizes custom
formulations, as long as a commercial powder base is
available as a reasonable starting point. Pricing for
special order media is as follows:
$20.00/liter for orders less than 10 L
$15.00/liter for orders 10 L or more
$13.00/liter for orders 10 L or more, and powder
provided by user
5. Drosophila Culture Medium
The Cell Center prepares media for Drosophila culture
in vials and bottles. Charge for a tray of 100 vials is
$18.00; a tray of 25 bottles costs $20.00.
6. Endotoxin Testing
We will test water or samples of media you have
prepared to determine endotoxin levels, using the LAL
(Lumilus Amebocyte Lysate) gel clot assay. At least
200 µl of sample should be submitted in an endotoxin
free container. Generally, radiation sterilized tissue
culture plasticware is endotoxin free, but autoclaved
glassware is not. Three dilutions per sample are
tested routinely, along with a set of blanks and positive
controls. Please indicate desired dilutions on the
request sheet. Prices for tests are: $15.00/sample for
1-2 samples, $10.00/sample for 3 or more samples.
There will be additional charges for testing blood or
serum samples because of time consuming boiling
and clarification steps required.
7. Mycoplasma Testing:
_ For the protection of personnel performing tests,
please clearly label supernatants from human cell lines
and virally infected or transformed cell lines.
_ Mycoplasma testing of cell lines that are to be introduced
into animals is strongly recommended by
ULAR. MHV testing is recommended for lines
passaged through, or to be introduced into mice. Both
tests are available at the Cell Center Service Facility.
7A. Positive/negative verification using fluorescent
DNA staining: $10.00 per sample ($20.00 per sample
for non-University of Penn. investigators) Requirements
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 18
are as follows:
a) All lines must be antibiotic-free for at least two
passages. Presence of antibiotics can give false
negative results.
b) Submit 1-2 ml of sterile supernatant (no cells) in a
15 ml screw cap tube. Presence of cells or cell
debris can produce false positive results. Supernatant
has to be in contact with the cells for at
least 48 hours.
c) All samples must be submitted by Noon on
Thursday, along with a request card.
d) Test results will be called in on the following
Tuesday or Wednesday.
7B. PCR assay for mycoplasma: The Cell Center will
perform a PCR test based on a set of five primer pairs
that produce unique fragments from the five most
common strains of mycoplasma (M. orale, M.
hyorhinis, M. fementans, A. laidlawi, M. arginini).
Products are analyzed by agarose gel electrophoresis.
Sample requirements are 0.2-0.5 ml of sterile culture
supernatant from cells grown without antibiotic. Cost
of the test is $25/sample for 1-2 samples, $20/sample
for 3 or more samples.
8. Mouse hepatitis virus testing: Cultured cells (e.g.
hybridomas) that are passaged through or intended to
be introduced into mice will be tested for mouse
hepatitis virus by RNA extraction from culture supernatant
and cell pellet, followed by RT-PCR. Please
provide a viable culture in a T25 flask. ULAR also
recommends that such lines be tested for mycoplasma
(see above). Cost of the RT-PCR test on both supernatant
and cell pellet is $100.
9. Lymphocyte isolation, EBV Transformation, DNA
extraction
Lymphocytes from patient blood samples are isolated
by centrifugation over Ficoll. These lymphocytes are
frozen and/or transformed by EBV and cultured until 3
vials can be frozen. Charge for lymphycte isolation
and freezing is $50/sample. Charge for EBV transformation
is $180. DNA can also be extracted from
blood, lymphocytes, or cultured EBV transformed cells
for a charge of $30. Contact facility for cost of larger
scale cultures, and reduced charges for large number
of samples.
10. Roller Bottle and Spinner Flask Facilities
Available free of charge if your personnel do the work,
or Cell Center personnel will do the work for a negotiated
fee.
11. Liquid Nitrogen Storage
The Cell Center rents space in liquid nitrogen freezers
at the rate of $1.00 per slot per quarter.
12. Autoclaving
The Cell Center will autoclave clean glassware and
properly packages biohazard waste at a charge of
$5.00 per cycle.
13. Hybridoma Production and Screening
The Cell Center will assist in monoclonal antibody
production, including consultation and development of
approaches appropriate to each research project,
antigen preparation, immunization, fusion, screening,
and cloning. We use a high efficiency fusion protocol
that results in at least 1000 hybridoma containing wells
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 19
per fusion. Typically, the Cell Center will perform
immunizations, fusion, collection of supernatants, and
subcloning of up to 20 positive lines for $2,000, while a
fusion involving subcloning of up to 40 positive lines
will cost $3,000. An additional $1,200 is charged for
an ELISA screen. Please see the “Fusion Request
form” and contact the Cell Center staff for more
information.
14. Production and Purification of Antibodies
The Center will produce and purify antibodies from
culture supernatant or ascites. Hybridoma cells can
be grown either in roller bottles or in hollow fiber
cartridges. Charge for a liter of culture supernatant
grown in roller bottles is $300; concentration by
precipitation and purification of an IgG antibody by
protein-G chromatography through FPLC cost $200..
We also do affinity purification of antibody against a
specific antigen; the cost of making the affinity column
is $100 (1 ml) or $150 (5 ml), the cost per run is $100.
If protein-free supernatant is required, a slow transition
to protein-free medium will be undertaken for a charge
of $200. Charges for hollow fiber culture are as
follows: the set up charge $450, charge per week $40
(0.1 - 0.5 g of antibody, depending on the hybridoma
line). Charges for ascites production are $100 per
mouse. Please note that some hybridoma lines will
not grow as ascites. Please consult with facility staff if
the line has not previously been grown as ascites.
Typical yield of successful ascites procedures is 3-6 ml
per mouse. ULAR strongly recommends that cells to
be used for ascites be tested for mycoplasma and
mouse hepatitis virus (see services #7 and 8 above).
15. Antibody isotyping, fragmentation, conjugation
Murine monoclonal antibodies are isotyped with a
commercial kit utilizing subclass specific antibody
reagents. Charge for isotyping is $20 per line. Fab and
F(ab)2’ fragment can also be produced by the facility,
as well as FITC, biotin, or enzyme conjugation of
purified antibody.
16. Anti-myc antibody
The facility provides protein G purified, ELISA tested
myc-1 monoclonal antibody from the 9E10 hybridoma
for use by University investigators at a cost of $30/mg.
17. ELISA reader
(Titertek’s Multiscan MC, in room D2 Richards). Free
of charge, and available to all members of the University
community, 9:00 a.m. - 4:30 p.m.
18. Cell Line Searches
The Cell Center has access to ATCC cell line and
hybridoma databases. The Cell Center will perform
searches for cells in these databases at your request
free of charge.
19. Other Shared Resources
The Cell Center is a shared resource and hybridoma
core facility for the Cancer Center. As much as
practically feasible, Cell Center equipment is available
for Cancer Center members’ use, particularly in pilot/
start up projects. This equipment includes:
• Sterile culture hoods, incubators, and accessory
equipment
• Roller bottle and spinner flask facilities
• Centrifuges
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 20
• Protein and DNA electrophoresis systems,
electrophoretic and positive pressure blotters
• UV cross-linker, transilluminator
• Speedvac concentrator and gel dryer
• Low pressure liquid chromatography system
• Zeiss fluorescence microscope and camera
• Leica inverted microscope and camera
• Sonic disruptor
• PEC DNA Cycler 480
• Zeiss 505E cryomicrotome
Summary Charge for Services
Charge for
Cancer Center
Service Description General Charge & Genetics
Antibody isotyping (per line) $30.00 $28.00
Antibody purification (protein G) $200.00 $180.00
Antibody purification
Affinity column (1 ml) $100.00 $90.00
Affinity column (5 ml) $150.00 $135.00
Cost per run $100.00 $90.00
Antibody conjugation $100.00 $90.00
Ascites (per mouse) $100.00 $90.00
Autoclave (cycles) $5.00 $4.50
Cell culture Large scale (1 L) $300.00 $270.00
Cell culture Large scale (hollow fiber)
Set up charge $450.00 $405.00
Charge per week (0.1-0.5gm of antibody) $140.00 $126.00
Cell Culture T150 $55.00 $49.50
Cell Culture T75 $45.00 $40.50
Cell Culture T25 $40.00 $36.00
DNA isolation (10-20 ml blood or 100 ml culture) $30.00 $27.00
Drosophila medium (100 vials) $25.00 $25.00
Drosophila medium (25 bottles) $25.00 $25.00
EBV stock $6.50$6.0 0
Endotoxin test (1-2 tests per test) $15.00 $13.50
Endotoxin test (3 or more tests per test) $10.00 $9.00
Fusion U Penn 20 clones $1900.00 $1710.00
Fusion U Penn 40 clones $2900.00 $1610.00
Fusion non-U Penn 20 clones $2600.00
Fusion non-U Penn 40 clones $4000.00
Fusion Screen ELISA $1200.00 $1080.00
Liquid N2 storage (per vial/quarter) $1.00 $0.90
Lymphocyte EBV Transformation $180.00 $162.00
Lymphocyte isolation $50.00 $45.00
MHV test RT-PCR $100.00 $90.00
Myc-1 antibody from 9E10 (1 mg) $30.00 $27.00
Mycoplasma test PCR (1-2 samples per sample) $25.00 $22.50
Mycoplasma test PCR (>3 samples per sample) $20.00 $18.00
Mycoplasma test Hoescht non-U Penn (per sample) $20.00
Mycoplasma test Hoescht U Penn (per sample) $10.00 $9.00
Personnel Training cell culture $300.00 $270.00
Phage Secreted scFv production (1L) $250.00 $225.00
Special Media per L (10L or more powder provided) $13.00 $11.70
Special Media per L (10L or more) $15.00 $13.50
Special Media per L (less than 10L) $20.00 $18.00
Transition to protein free medium (per line) $200.00 $180.00
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 21
GENETIC
DIAGNOSTIC
LABORATORY
Haig H. Kazazian, Jr., M.D., Medical Director
215/898-3582
kazazian@mail.med.upenn.edu
Arupa Ganguly, Ph.D., Director, Research and Development
215/573-3020
ganguly@mail.med.upenn.edu
Catherine Stolle, Ph.D., Managing Director
215/573-3030
stolle@mail.med.upenn.edu
Lynn Godmilow, M.S.W., Director, Genetic Counseling
215/573-9161
godmilow@mail.med.upenn.edu
Location:
415 Anatomy Chemistry Building
339 Anatomy Chemistry Building
Mailing Address:
502 Clinical Research Building
415 Curie Boulevard
Philadelphia, PA 19104-6145
Telephone: 215/573-9161
Fax: 215/573-5940
Internet: http://www.med.upenn.edu/gdrs
Genetic Diagnostic
Laboratory
About the Genetic Diagnostic Laboratory
The Genetic Diagnostic Laboratory in the Department of
Genetics is pleased to offer state-of-the-art DNA Diagnostic
Testing and consultation for a wide variety of genetic
diseases and conditions. Pre-test genetic counseling,
rapid communication of results and accurate interpretation
with recommendations are available.
For more information about the available diagnostic tests,
sample requirements, and the protocols for requesting
tests, please contact Lynn Godmilow, MSW, CGC, Genetic
Counselor and Director of our Genetic Diagnostic Referral
Service by telephone (215/573-9161), fax (215/573-5940),
or by email (godmilow@mail.med.upenn.edu)
Available Molecular Tests for Genetic Disorders
Adenosine Monophosphate Deaminase-1
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 22
(AMPD1)
Background: Deficiency of this muscle specific enzyme is a
common cause of exercise-induced myopathy
characterized by weakness and cramping
and probably the most common cause of
metabolic myopathy in humans.
Assay: Direct mutation by PCR
Utility: To detect carriers and affected individuals as
part of the differential diagnosis of exercise
induced myopathy
CPT codes: 83890, 83892, 83894, 83898, 83912
Turnaround: 1 week
•-Thalassemia
Background: Autosomal recessive disease found mostly in
Southeast Asians caused by deletion of aglobin
genes with severity dependent on the
number of deleted genes and leading to
lifelong dependence on transfusions with iron
buildup and shortened life-span. In AfricanAmericans deletion of fewer alpha genes than
in Southeast Asians can be clinically similar
to ß-thalassemia trait.
Assay: Direct mutation by Southern blot. Direct
detection of Hb Constant Spring if indicated
Utility: Confirmation of diagnosis, carrier detection,
prenatal diagnosis
CPT codes: 83891, 83892, 83894, 83896, 83897, 83898,
83904, 83912
Turnaround: 2-3 weeks
ß-Thalassemia (Cooley’s Anemia, Mediterranean
Anemia)
Background: Autosomal recessive disease, common in the
Mediterranean, Asian, Indian, Middle Eastern
and African-American populations, caused by
decreased or absent ß-globin chains;
characterized by severe anemia, growth
retardation, splenomegaly, bone changes with
characteristic facies and iron depositions
affecting cardiac and endocrine systems.
Assay: Direct mutation by PCR or multiple PCR’s
Utility: Confirmation of diagnosis, carrier detection,
prenatal diagnosis
CPT codes: 83890, 83891, 83892, 83896, 83897, 83898,
83904, 83912
Turnaround: 2 weeks
CADASIL
Background: CADASIL (cerebral autosomal dominant
arteriopathy with subcortical infarcts and
leukoencephalopathy) is characterized by
history of migraine headaches, mid-adult
(30s-60s) onset of cerebrovascular disease
progressing to dementia, and diffuse white
matter deficits on neuroimaging. CADASIL is
caused by mutations in the Notch3 gene
(chromosome 19).tems.
Assay: Direct mutation analysis by Conformation
Sensitive Gel Electrophoresis (CSGE).
Analysis of PCR products for the presence of
heteroduplexes followed by sequencing to
detect differences from the consensus
sequence of Notch3. There are three levels
of testing performed. Level 1 consists of
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 23
commonly mutated exons and will detect 87%
of mutations in skin biopsy proven cases.
Level 2 consists of testing all other exons in
which a mutation has been reported and will
improve sensitivity to 93% in biopsy proven
cases. Level 3 consists of testing the remaining
exons encoding EGF like domains and
will be performed for a limited time on a
research basis at no additional cost to the
patient. There is currently no information
regarding the increase in sensitivity (if any)
with Level 3 testing.
Utility: Diagnostic confirmation, prenatal diagnosis,
presymptomatic testing (presymptomatic
testing is available by special arrangementtalk
with the genetic counselor)
CPT codes: Level 1- 83890, 83898, 83898-76, 83903,
83904, 83904-76, 83912; Level 2 - 83898,
83898-76, 83903, 83904, 83904-76
Turnaround: 2 weeks for Level 1; 2weeks for Level 2
Charcot-Marie-Tooth Disease (X-linked)
Background: Sex linked dominant neurodegenerative
disorder .
Assay: Sequencing of the connexin 32 gene detects
mutations in >85% of patients.
Utility: To confirm the clinical diagnosis and to offer
prenatal diagnosis
CPT codes: 83890, 83898, 83894, 83904, 83912
Turnaround: 4 weeks
Craniosynostosis Syndromes: Apert, Coronal
Synostosis (Plagiocephaly), Crouzon, JacksonWeiss, Pfeiffer
Background: Highly penetrant, autosomal dominant
disorders characterized by premature fusion
of skull bones leading to an abnormal head
shape, ocular hypertelorism with proptosis,
midface hypoplasia, and the presence or
absence of characteristic hand and/or foot
manifestations. Although many cases are
sporadic, familial cases have been reported.
Diagnosis is possible on the basis of clinical
exam in many cases, however, variable
expressivity can result in ambiguity or
misdiagnosis and, prenatal diagnosis based
on ultrasound is unreliable. These disorders
are due to mutations in fibroblast growth
factor receptors (FGFR1, 2, 3).
Assay: Direct mutation detection by restriction
enzyme digestion or sequence analysis of
PCR-amplified genomic DNA.
Utility: Confirm a presumed clinical diagnosis or for
prenatal diagnosis in familial or sporadic
cases.
CPT Codes: Apert: 83890, 83892, 83894, 83898, 83912
Coronal Synostosis: 83890, 83892, 83894,
83898, 83912
Crouzon: 83890, 83894, 83898, 83904,
83912
Jackson-Weiss: 83890, 83894, 83898, 83904,
83912
Pfeiffer: 83890, 83892, 83894, 83898, 83904,
83912
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 24
Turnaround: 1-2 weeks
Duchenne/Becker Muscular Dystrophy (DMD/
BMD)
Background: Duchenne Muscular Dystrophy is an X-linked
progressive neuromuscular disorder beginning
in childhood with muscle weakness
followed by wheel chair dependence and
leading to a shortened life span. The defect
has been shown to reside in the gene coding
for a protein called dystrophin. The milder
Becker form, also X-linked, is less common,
has later onset with a milder course and
these individuals generally have normal lifespans.
One third of the cases are new
mutations. Sixty-five to seventy percent of
patients demonstrate a deletion or in some
cases a duplication.
Assay: Direct analysis in males first via mutiplex
PCR amplification of multiple dystrophin
exons followed by Southern blot analysis with
cDNA probes if indicated. Carrier detection in
females by dosage analysis for deletions or
by linkage analysis using intragenic and
flanking polymorphic markers.
Utility: Confirmation of diagnosis, carrier detection,
prenatal diagnosis
CPT codes: For PCR: 83890, 83894, 83901, 83912
for Southern: 83891, 83892, 83896, 83897,
83912
For linkage: 83891, 83892, 83894, 83896,
83897, 83898, 83912
Turnaround: PCR 1 week, Southern 3 weeks, Linkage 4
weeks
Familial Adenomatous Polyposis (FAP)
Background: Familial adenomatous polyposis (FAP) is an
autosomal dominant disorder which typically
presents with colorectal cancer in early adult
life secondary to extensive adenomatous
polyps of the colon. Polyps also develop in
the upper gastrointestinal tract and malignancies
may occur in other sites including the
brain and the thyroid. Helpful diagnostic
features include pigmented retinal lesions
known as congenital hypertrophy of the
retinal pigment, jaw cysts, sebaceous cysts,
and osteomata. Screening for a mutation in
the coding region of the APC gene is offered
to individuals who have multiple colonic
polyps. A pedigree demonstrating all cancers
in the family must be submitted with the
sample. Family members of individuals in
whom a mutation has been identified can be
studied to determine if they inherited the
familial mutation.
Assay: Conformation Sensitive Gel Electrophoresis/
Sequencing (analysis of PCR products for the
presence of heteroduplexes followed by
sequencing to detect differences from the
consensus sequence of APC).
Utility: To identify individuals at very high risk of
developing colon cancer so that they can be
targeted for aggressive prevention programs
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 25
and to reassure individuals in families with a
known mutation that they are not at any
higher risk than individuals in the general
population (1-25 lifetime risk).
CPT codes: Screening: 83891, 83898, 83903, 83904,
83912
Familial Mutation: 83890, 83894, 83898,
83903, 80904, 83912
Turn around: 8 weeks for screening, 2 weeks for familial
mutation
Fragile X Syndrome
Background: This is the most common cause of mental
retardation in males and also a common
cause of learning disabilities and emotional
dysfunction in males and females. Carriers
can be mentally retarded or normal. The
disorder becomes clinically manifest when
the mutation (an expansion of a CGG
nucleotide repeat) reaches a threshold of
600bp.
Assay: Direct mutation analysis by Southern blot.
Utility: Confirmation of clinical diagnosis, carrier
detection, prenatal diagnosis
CPT codes: 83891, 83892, 83896, 83897, 83912
Turnaround: 2 weeks
Friedreich Ataxia (FRDA)
Background: Friedreich Ataxia is the most common
hereditary ataxia with an estimated prevalence
of 1 in 50,000 and a carrier frequency
of 1 in 100 in the European population. It is
an autosomal recessive disorder characterized
by progressive gait and limb ataxia, lack
of tendon reflexes in the legs, loss of position
sense, dysarthria, and pyramidal weakness of
the legs. Hypertrophic cardiomyopathy is
found in almost all patients and diabetes
mellitus is seen in 10% of the patients. The
age of onset is usually around puberty, almost
always before 25 years with a slow progression
of the disease. The disease is due to
expansion of an unstable GAA trinucleotide
repeat in intron 1 of the frataxin gene.
Assay: Direct mutation detection by long range PCR
and/or Southern blot, sequence analysis for
point mutation if indicated. Estimated 95%
detection rate.
Utility: Confirmation of diagnosis in a symptomatic
individual, predictive testing for asymptomatic
individuals with a positive family history,
carrier testing, and prenatal testing.
CPT Codes: 83891, 83892, 83894, 83896, 83897, 83898,
83904, 83912
Turnaround: 3 weeks
Hemophilia A
Background: This is the most common severe bleeding
disorder which is inherited as a classic Xlinked
recessive. In half of all cases of
severe Hemophilia A (factor VIII <1%) an
inversion is detectable. In those cases where
the inversion is not present or if the
Factor VIII level is not <1%, direct mutation
analysis and linkage analysis are available.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 26
Assay: Direct detection of the inversions by RT-PCR
and direct mutation analysis by CSGE
[Conformation Sensitive Gel Electrophoresis /
Sequencing. PCR amplified products,
including all coding exons and the exon-intron
boundaries, are analyzed by electrophoresis
in a novel polyacrylamide gel system for the
presence of aberrantly migrating DNA
fragments indicating sequence differences
from the consensus sequence of Factor 8.
Utility: Diagnostic confirmation, carrier detection,
and prenatal diagnosis
CPT codes: Inversion detection: 83891, 83892, 83896,
83897, 83912
Direct mutation analysis: 83891, 83898,
83903, 83904, 83912
Known mutation: 83890, 83894, 83898,
83903, 83904, 83912
Linkage: 83890, 83892, 83894, 83896,
83898, 83912
Turnaround: inversion testing 2 weeks, known mutation 2
weeks, direct mutation screen 2-15 weeks,
linkage 3-4 weeks
Hereditary Papillary Renal Carcinoma (HPRC)
Background: Hereditary papillary renal carcinoma is a
recently recognized form of inherited kidney
cancer due to a mutation in the MET protooncogerne
characterized by a predisposition
to develop multiple bilateral papillary renal
tumors. The pattern of inheritance is consistent
with autosomal dominant transmission
with reduced penetrance.
Assay: PCR amplification, mutation scan by CSGE
and sequence analysis of abnormal bands for
those four exons where all mutations discovered
to date were found.
Utility: Early diagnosis so that increased surveillance
could detect tumors early.
CPT codes: 83890, 83894, 83898, 83903, 83904, 83912
Turnaround: 4 weeks
HNPCC-Mutation Analysis of MSH2 and MLH1
(HNPCC/Muir Torre Syndrome)
Background: Colorectal cancer (CRC) occurs in an
estimated 130,000 patients in the United
States each year. Five to ten percent of all
CRC cases are thought to be inherited and
the remainder is sporadic. The most common
form of hereditary CRC is HNPCC (hereditary
nonpolyposis colon cancer). Approximately
70% of families that meet the established
Amsterdam and Bethesda diagnostic criteria
will have a mutation in one of the 4 genes,
which have been identified so far in HNPCC.
Over 90% of these will have a mutation in
MSH2 or MLH1.
Eligibility: Patients who have or have had CRC and who
meet the Amsterdam or Bethesda criteria are
eligible for testing. Also isolated patients with
CRC and sebaceous skin cancer may carry
mutations in MSH2 and are eligible for
testing.
The Amsterdam criteria are defined as 1) at
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 27
least two successive generations should be
affected with CRC; 2) diagnosis of one
affected individual before the age of 50; 3)
histologically verified CRC in at least three
relatives, one of whom is a first-degree
relative of the other two.
The Bethesda criteria consist of modifications
of the Amsterdam criteria and include 1) very
small families which cannot be expanded
further with two documented cases of CRC or
families with 2 first degree relatives with CRC
and the presence of a third relative with an
early-onset unusual neoplasm or endometrial
cancer; 2) age 55 instead of 50 as an upper
limit of early-onset cancer; and 3) verification
of cancer may be death certificates or clinical
charts.
Assay: Conformation Sensitive Gel Electrophoresis
(CSGE) Analysis of PCR products for the
presence of heteroduplexes followed by
sequencing to detect differences from the
consensus sequence of MSH2 and MLH1.
Utility: To identify individuals at high risk of developing
CRC, endometrial and other cancers so
that they can be targeted for regular screening
by colonoscopy and endometrial biopsy
and to reassure other individuals in families
with known mutations that they are not at any
higher lifetime risk than individuals in the
general population (6%).
CPT codes: Screening-83891, 83898, 83903, 83904,
83912
Familial Mutation-83890, 83894, 83898,
80903, 83904, 83912
Turnaround: 8-12 weeks for screening, 2 weeks for familial
mutation
Huntington’s Disease (HD)
Background: A severe autosomal dominant
neurodegenerative disorder causing progressive
choreiform movements and often
dementia in mid-adulthood. The molecular
defect is expansion of a CAG trinucleotide
repeat.
Assay: Direct detection by PCR analysis and gene
scan of the number of CAG repeats in the
Huntington gene. The primers used do not
include an adjacent polymorphic region.
Utility: Diagnostic confirmation, prenatal diagnosis,
presymptomatic testing (presymptomatic
testing is only available through the
Neurogenetic Center Presymptomatic
Huntington’s Disease Program or by special
arrangement-talk with the genetic counselor)
CPT codes: 83890, 83894, 83898, 83912
Turnaround: 2 weeks
Li-Fraumeni Syndrome (P53)
Background: This is an autosomal dominant cancer
predisposition syndrome which predisposes
to multiple forms of cancer (breast cancer,
soft tissue sarcoma, leukemia, melanoma,
osteosarcoma, colon cancer, pancreatic
cancer, esophageal cancer, brain tumors and
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 28
others). The syndrome is due to germ line
mutations in the p53 gene. This disorder is
defined as: a proband with a sarcoma under
45 years of age and a first degree relative
with any cancer under 45 years of age and a
third family member who is a first- or seconddegree
relative with cancer under 45 years of
age or a sarcoma at any age.
Eligibility: Patients who have or have had one of the
index cancers and whose family history
meets the Li-Fraumeni criteria are eligible for
screening. Individuals from families where a
mutation has already been identified are
eligible for specific mutation testing.
Assay: Conformation Sensitive Gel Electrophoresis
(CSGE). Heteroduplex analysis of PCR
products of genomic DNA of the p53 in a
novel polyacrylamide gel for the presence of
heteroduplexes. Differences between the 2
alleles of an individual as small as a single
base substitution, insertion or deletion are
detected as 2 bands. Sequencing follows the
preliminary screening to identify the exact
nature of the alteration.
1. C.J.Williams, M.Rock, E.Considene,
S.McCarron, P.Gow, R.Ladda, D.Mclain,
V.Michels, W.Murphy, D.J.Prockop and
A.Ganguly. (1995) Hum. Mol. Genet. 4,
309-312.
2. A.Ganguly, M.J. Rock and D.J.Prockop
(1993) Proc. Natl. Acad. Sci . U.S.A. 90,
10325-10329.
3. A. Ganguly and C. Williams. (1997)
Hum.Mutat. 9, 339-343.
Utility: Diagnostic confirmation, carrier detection,
and prenatal diagnosis
CPT codes: Direct mutation analysis: 83890, 83894,
83898, 83898-76, 83912
Familial Mutation-83890, 83898, 83898-76,
83912
Turnaround: 10-14 weeks
Multiple Endocrine Neoplasia (MEN2A)
Background: MEN2A is an autosomal dominantly inherited
cancer syndrome characterized by medullary
thyroid carcinoma, pheochromocytoma, and
parathyroid hyperplasia. In 97% of MEN2A
patients, the mutation involves one of five
cysteine residues in the extracellular domain
of the RET proto-oncogene.
Assay: PCR amplification of genomic DNA, restriction
enzyme digestion, and sequencing of
exons 10, 11, 13 & 14.
Utility: Testing is useful to confirm a clinical diagnosis
or to identify presymptomatic carriers in a
family.
CPT codes: 83890, 83894, 83898, 83904, 83912
Turnaround: 2 weeks
Multiple Endocrine Neoplasia 2B (MEN2B)
Background: MEN2B is an autosomal dominantly inherited
cancer syndrome characterized by bilateral
medullary thyroid carcinoma, pheochromocytoma,
diffuse ganglioneuromas of the
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 29
intestinal tract, mucosal neuromas, and
skeletal abnormalities. Symptoms of MEN2B
are evident within the first decade of life and
progresses rapidly. In greater than 95% of
cases, there is a mutation at a single position
within the extracellular ligand-binding domain
of the RET proto-oncogene.
Assay: PCR amplification of genomic DNA and
restriction enzyme digestion.
Utility: Confirm clinical diagnosis; identify
presymptomatic carriers prior to prophylactic
thyroidectomy.
CPT Codes: 83890, 83892, 83898, 83912
Turnaround: 1 week
Retinoblastoma
Background: Retinoblastoma (RB) is a malignant tumor of
the developing retina that occurs in children,
usually before the age of five years. Retinoblastoma
may be unilateral or bilateral. About
60% of patients have unilateral RB with a
mean age of diagnosis of 24 months; about
40% have bilateral RB with a mean age of
diagnosis of 15 months. Mutations in the RB
gene (chromosomal location 13q14) predispose
individuals to the disease and an
increased risk of developing other RB-related
(non-ocular) tumors.
Assay: Direct mutation analysis by CSGE [Conformation
Sensitive Gel Electrophoresis] and
sequencing. PCR amplified products,
including all coding exons and the exon-intron
boundaries, are analyzed by electrophoresis
in a novel polyacrylamide gel system for the
presence of aberrantly migrating DNA
fragments indicating sequence differences
from the consensus sequence of RB1.
Mutation analysis by CSGE of the RB gene
can identify a germline mutation in about 70%
of individuals with a hereditary predisposition
to RB. The probability that an RB1 gene
mutation will be detected in an index case
depends upon whether the tumor is unilateral
or bilateral and whether the family history is
positive or negative.
Utility: Diagnostic confirmation, carrier detection and
prenatal diagnosis
CPT codes: 83891, 83898, 83898-76 (x8), 83904, 8390476 (x8), 83910
Sensitivity: If the patient has a mutation in the coding
sequencing of the RB1 gene, the testing will
detect the mutation ~70% of the time.
Mutations in non-coding sequences, insertions,
deletions or rearrangements will not be
detected by sequencing.
Sickle Cell Anemia (Hb SS or SC)
Background: Autosomal recessive disorder of red blood
cells which causes them to become distorted
leading to blockage of capillaries, pain and
damage to various organ systems.
Assay: Direct mutation detection by PCR
Utility: Confirmation of carrier or affected status and
prenatal diagnosis
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 30
CPT codes: 83890, 83892, 83894, 83894, 83898, 83912
Turnaround: 1 week
von-Hippel Lindau (VHL)
Background: von-Hippel Lindau disease is a potentially
lethal autosomal dominant disorder predisposing
to benign and malignant tumors of the
brain, spine, retina, pancreas and kidneys.
Sensitivity is 93%.
Assay: Rearrangement by Southern and/or direct
mutation detection by sequence
Utility: Early diagnosis so that increased surveillance
could detect tumors early and prevent death
GENETIC DIAGNOSTIC LABORATORY
25
from hemangioblastomas and renal cell
carcinomas, prenatal diagnosis
CPT codes: Southern blot: 83891, 83892, 83896, 83897,
83912
Sequence:83891, 83894, 83898, ,
83903, 83904, 83912
Known point mutation: 83890, 83892,
83894, 83898, 83904, 83912
Turnaround: 2 weeks for Southern, 2 weeks for sequence,
2 weeks for familial mutation
U N I V E R S I T Y O F P E N N S Y LVA N I A
SCHOOLOFMEDICINE
TRANSGENIC &
CHIMERIC MOUSE
FACILITY
Nancy E. Cooke, M.D., Facility Co-Director
215/898-4425
necooke@mail.med.upenn.edu
Stephen A. Liebhaber, M.D., Facility Co-Director
215/898-7834
liebhabe@mail.med.upenn.edu
Jean Richa, Ph.D., Technical Director
215/898-6064; 215/573-3023
jricha@mail.med.upenn.edu
Pei Fu He, Ph.D., Research Specialist
215/573-3023
peifu@mail.med.upenn.edu
Kathleen A. Thompson, Research Specialist
215/573-3023
kthompso@mail.med.upenn.edu
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 31
Location:
32 Clinical Research Building
Mailing Address:
502 Clinical Research Building
415 Curie Boulevard
Philadelphia, PA 19104-6145
Telephone: 215/573-3023
Fax: 215/573-5892
Internet: http://www.med.upenn.edu/tcmf
The Transgenic and Chimeric Mouse Facility provides a centralized service of transgenic mouse production to
the Penn community. It offers services such as DNA injection into fertilized oocytes, ES cell injection into
blastocysts, line rederivation, and embryo cryopreservation, along with the care and housing of the mice up to
the stage where they will be transferred to the participating investigator. The facility is fully equipped to carry
out these procedures. The facility is located behind a microbiologic barrier where admittance is restricted and
all food, water, cages, and bedding are autoclaved before use.
2004 GENETICS CORE FACILITIES CATALOG
28
Introduction
The Transgenic Mouse System
Integration of genes into the mouse genome can serve as
an experimental system to study normal as well as altered
gene expression. Such studies can also serve to identify
genes essential for normal development and generate
animal models for human diseases.
Direct microinjection of DNA into the male pronucleus of a
mouse zygote has been the method most extensively used
in the production of transgenic mice. If the foreign DNA
integrates into the mouse chromosomal DNA at the onecell
stage, the transgenic animal will contain the injected
DNA in every cell, including those of the germ line. If,
however, integration occurs later, the resulting animals will
be mosaic for the presence of the injected DNA. The
number of copies of integrated DNA can vary from one to
several hundred, arranged primarily in tandem head to tail
arrays.
The production of chimeric mice by introducing Embryonic
Stem (ES) cells into early embryos is an alternative
approach to generating transgenic mice. This technique
utilizes the tissue culture system to modify and select ES
cells that have received an exogenous DNA of interest.
Once derived and characterized, ES cell clones may be
transferred into 4-day-old mouse embryos where they can
differentiate into any adult tissue type. The choice of
mouse strain is of great importance: donor embryos that
would generate ES cells and host embryos that would
receive transferred cells are obtained from different strains
of mice carrying different eye or coat pigmentation. This
facilitates the visual distinction between cell populations in
the adult “chimeric mouse”.
The Facility
The purpose of the Transgenic & Chimeric Mouse Facility
is to provide a centralized service to efficiently produce
transgenic mice for basic research. This should result in
reduction in effort and cost to participating investigators.
The facility is located on the basement level of the Clinical
Research Building. This facility consists of an injection
room and several animal rooms. The injection room is fully
equipped to carry out the entire procedure of making
transgenic mice. The animal rooms provide housing and
breeding space for the mice involved in the transgenic
projects. The facility uses sterile food and water as well as
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 32
autoclaved cages and bedding; all cages are of the
microisolator type to limit the spread of colony infection.
The entire facility is located behind a microbiologic barrier
where admittance is strictly limited and all personnel must
wear sterile coveralls, gloves, hats, masks, and boots.
Personnel
Nancy E. Cooke, M.D., is the Facilitity Director and
Stephen A. Liebhaber, M.D., is the Facility Co-Director.
Jean Richa, Ph.D., is the Technical Director in charge of
the production of transgenic mice. Dr. Richa will maintain
direct contact with investigators using facility, coordinate
ULAR interactions and facility maintenance, and be directly
responsible for scientific consultations relevant to faiclity
use. Pei-Fu He, Ph.D. and Kathleen A. Thompson,
Research Specialists, assist in various aspects of the
facility’s services.
What the Facility will Provide
DNA Injection
Upon receipt of the DNA construct, proven by
documentation to be of high quality and suitable for
microinjection (see below), we will proceed to inject a
minimum of 150 fertilized eggs. After overnight incubation,
normal 2-cell embryos will be transferred into foster
females and their pregnancy will be monitored (the
gestation period of the mouse is 19-20 days). Two weeks
after birth of potential transgenic pups, the project scientist
will be notified of the number of pups. The facility will carry
out tail clipping and animal tagging prior to weaning. Tail
biopsies will be conveyed to the project scientist for
analysis by Southern blotting, PCR, or both. At this point,
the clipped and tagged pups will be transferred to the care
of the individual investigator who will be responsible for
subsequent maintenance. Since cage costs are high and
storage space is limited, investigators should make certain
that DNA screening procedures are working prior to
initiating transgenic work with the facility. Investigators
must report the results of screening with documentations
(e.g., gel photo, etc.) to the Technical Director. This
information is necessary for facility personnel to maintain
and improve their efficiency. If these studies (with
adequate controls) fail to demonstrate the existence of at
least one transgenic animal, we will reinject at least 80
eggs at no additional cost to the investigator. If, however,
no transgenic animal is detected after the second trial, a
meeting will be held between the scientist, the Facility CoDirectors, and the Technical Director to discuss the DNA
construct, the tail biopsy analysis, and further plans to be
agreed upon. On average, 230 injected eggs should yield
at least one (and usually more) transgenic mouse.
It is well understood that many factors can affect the
production efficiency of transgenic mice. Such factors
include:
_ Number of eggs surviving the injection and developing
to 2-cell stage; this will determine the number of
transfers into foster females. It is important to thave a
DNA preparation pure of any contaminant and at the
appropriate concentration so that toxic effects to the
eggs can be avoided.
_ Successful pregnancies: although every transfer
promises a certain number of pups, that number may
vary greatly due to embryo death in utero. This
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 33
lethality may be closely associated with the type of
DNA construct used.
Transient transgenics
For investigators who wish to examine DNA integration and
expression in transgenic embryos, we can provide similar
DNA injection schedule for a fee of $855.00. We will inject
150 eggs, transfer the developing embryos into foster
females, and arrange with the project scientist to receive
the pregnant females on the asssigned date. We would
still need to receive a report on the number of embryos
harvested from each female, and the frequency of
transgenics among them.
ES cell injection
Through this service, the facility will generate chimeric mice
by injecting Embryonic Stem (ES) cells containing site
specific genomic alterations (knockouts) into normal
blastocysts. ES cells will be injected into a minimum of 50
blastocysts of C57BL/6J. When the blastocysts have
reexpanded, they will be transferred into the uterine horns
of pseudopregnant females and allowed to go to term.
Upon weaning 3 weeks after birth, chimeric mice are
separated and then released to the care of the investigator.
Due to the fact that this approach is time-consuming, it is
recommended that certain guidelines be followed in order
to minimize loss of time and reduce risk factors. These
guidelines include:
_ Before initiating the injection of the recombinant cell
line, normal non-recombinant ES cells should be
tested for their chimera-producing potential.
_ A mycoplasma test of the recombinant line is
absolutely necessary since the presence of such
contamination has been associated with loss of
pregnancy and reduced litter size.
_ On the assigned day, the freshly harvested ES cell
sample (free of feeder cells) will be delivered to Dr.
Jean Richa to proceed with this injection.
_ A photocopy of the recombinant ES cell line screening
results (Southern blot or PCR) should be submitted
along with the chimeric mice request form (see page
13).
Embryo cryopreservation
Freezing mouse embryos is a convenient way to preserve
in important mouse line for possible future use or to protect
this line against loss due to infection, lab accident, etc.
Embryo freezing could also result in substantial reduction
in housing cost and cage use
This newly added service is now available to interested
investigators. The service can be designed to suit the
needs of each investigator. However, a general scheme is
outlined below.
A set of five young male mice will be needed to generate
enough embryos for each freezing set. The male founders
will be subjected to a waiting period in the containment
area, as required by ULAR, prior to admission into the
barrier colony.
A minimum of 300 healthy embryos will be frozen per
transgenic/knockout line that is hemizygous for the
transgene. In the case where the mouse line is
homozygous for the transgene, 150 embryos will suffice.
Several weeks after the completion of the freezing process,
a few embryo samples will be removed and processed
through thawing and monitoring development to assess
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 34
their viability and ability to develop to term, Once these
tests are completed, the frozen embryos will be submitted
to the care of the investigator for proper storage.
High efficiency of embryo freezing will depend primarily on
the reproductive level of the breeders since low mating
frequency and low percentage of fertilization will lead to a
prolonged time-consuming process.
Mouse line rederivation
This process involves the surgical transfer of embryos into
pathogen-free recipient females, where they can develop to
term. Rederivation is the most effective method to rid a
colony of pathogenic infections. It is also recommended
when genetically altered animals are acquired from outside
institutions that do not fall into the category of approved
vendors. With the proper procedure, mouse lines can be
saved or transferred safely across distant locations.
Due to the diverse environmental conditions among the
animal facilities located across campus, the structure of
this service will be tailored to fit each and every facility
receiving the rederived line. As a consequence, the fees
may vary accordingly. Basically, a total of 50 embryos
generated from the designated mouse line will be
transferred into recipient pathogen-free females that would
carry them to term in a housing location assigned by
ULAR. The mice born from such transfers should be of
sufficient number to rejuvenate or establish the line.
Investigator’s Responsibilites
For DNA injection
In general terms, the investigator must prepare the DNA to
be injected and assume responsibility for screening and
care of the weaned animals. Specifically, in order to
provide you with this service, it is requested that you:
1) Contact Jean Richa (215/898-6064) to discuss the
DNA construct and the schedule of injections.
2) Provide the facility with a high-quality DNA preparation
of your construct with documentation of its purity. A
collection of four tubes appropriately labeled and each
containing 25 µl of DNA at 5 ng/µl concentration would
suffice. (The recommended protocol for DNA
preparation can be found in the Appendix.)
3) Fill out and sign a service request form, including
evidence of approval of the IACUC and IBC
committees for the project. Unless you have been
doing transgenic mouse work, this will require a new
submission to both committees. A sample of such a
form is on page 11 of this manual. Feel free to
duplicate it as you need it.
4) Provide a timely and well-controlled analysis of the tail
biopsies so that transgenic animals can be identified
appropriately and your cage costs can be minimized.
5) Undertake the care of the potential transgenic pups
submitted to the project scientist along with the tail
biopsies. These mice can no longer reenter the barrier
colony. It is the responsibility of the individual
investigator to arrange for animal housing.
For ES cell injection
The investigator is responsible for maintaining the
recombinant ES clone under optimal conditions that would
inhibit their differentiation. Once the chimeric animals are
weaned, it is the responsibility of the investigator to breed
them for germ line transmission testing. It is recommended
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 35
that you:
1) Contact Jean Richa (215/898-6064) to arrange for
testing the non-recombinant ES cell line and discuss
the schedule of injection.
2) Fill out and sign a service request form, including
evidence of approval of the IACUC and IBC
committees for the project. Unless you have been
involved with chimeric mouse work, this will require a
new submission to both committees. A sample of such
form is on page 13 of this manual. Feel free to
duplicate it as you need it.
3) Upon notification of a prearranged schedule, provide
the facility with a freshly harvested sample of ES cells
along with an aliquot of HEPES-buffered ES cell
culture medium (5 ml). The cell sample should be free
of feeder cells. Additional information on maintaining
and harvesting ES cells could be found in
Teratocarcinomas and Embryonic Stem Cells (E.J.
Robertson, Ed.), IRL Press, Oxford, 1987, as well as in
Meth. Enz. v. 225, 1993.
4) Undertake the care of the weaned 3-week-old chimeric
mice submitted to the project scientist. These mice
can no longer reenter the barrier colony. It is the
responsibility of the principal investigator to arrange for
animal housing.
5) Relay to the facility any information about germ line
transmission once such information becomes
available.
For Embryo Cryopreservation
The investigator should contact Jean Richa (215/898-6064)
to discuss the plans for the service. These would include
the availability of five breeding males, the possibility of
admitting the animals into the barrier facility, the number of
mouse lines involved, and the service fees.
For Line Rederivation
The investigator should contact Jean Richa (215/898-6064)
to discuss the plans for the service. These would include
the availability and housing location of the line(s) to be
rederived, as well as the service fee. The investigator
should be responsible for the housing and care of the
recipient females while they are in transition in the location
assigned by ULAR.
Costs
We will charge a fee of $1,100.00 per DNA construct
injected for the production of transgenic mice. The
initiation of zygote injections depends upon receipt of an
approved protocol and request form accompanied with
properly labeled tubes of the DNA construct and
documentation of its purity. Currently, we harvest fertilized
eggs from superovulated B6SJLF1/J females mated with
B6SJLF1/J males (from JAX). We will attempt to
accommodate requests for other mouse strains if required
by specific experiments. Please justify these requirements
in your protocol for review. Additional costs will be
incurred. In the case of DNA injection for transient
transgenics, the fee will be $855.00 per DNA construct.
For ES cell injection, a fee of $1,100.00 will be charged per
cell line injected for the production of chimeric mice. The
initiation of blastocysts injections depends upon receipt of
an approved protocol and request form, following a
schedule prearranged with Dr. Jean Richa. The schedule
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 36
will be discussed after testing the ES cell line and
approving the documentation of the recombination event in
the assigned ES recombinant clone. Currently, we harvest
blastocysts from C57BL/6J females mated with C57BL/6J
males (from JAX). An initial, one time fee of $300.00 is
charged for testing the non-recombinant ES cell line for its
ability to contribute to chimerism.
The full cost of the transgenic service (DNA/ES) is
$3,780.00 per construct/cell line. The low fee of $1,100.00
for Medical School investigators, and members of the
Cancer Center, Diabetes Center, and Digestive and Liver
Diseases Center, is possible due to a substantial subsidy
by those organizations. For all other Penn investigators,
the cost of either service is $2,200.00 per construct/cell
line.
For embryo cryopreservation, the cost will depend primarily
on the successful reproduction of the breeding males
provided. A minimum estimate of $1,100.00 is projected,
with the cost of the quarantine housing being the
responsibility of the investigator.
For line rederivation, the cost will depend primarily on the
source location as well as the destination of the mouse line
to be rederived. A minimum estimate of $550.00 is
projected.
Policies
Committee Approval
It is an Institutional requirement that every investigator
demonstrate an updated approval by the Institutional
Animal Care and Use Committee (IACUC) as well as the
Institutional Biosafety Committee (IBC) for both DNA and
ES cell injection projects prior to using the Transgenic &
Chimeric Animal Facility.
Scheduling
It is our policy that no injection date will be assigned to any
DNA construct until it is submitted along with a completed
TRANSGENIC & CHIMERIC MOUSE FACILITY
31
service request form and the required documents attached.
Confidentiality
All information provided by the investigator concerning the
research project are treated with the utmost confidentiality.
No subject related material will be released to or shared
with anyone without a written consent of the principal
investigator.
Support Letter
For those investigators preparing to submit grant proposals
to funding agencies, we will be happy to provide you with a
letter stating our readiness to make transgenic and/or
chimeric mice for your project.
Acknowledgments
We request that any scientific publications involving these
transgenic animals acknowledge the service of the
University of Pennsylvania Transgenic & Chimeric Mouse
Facility in the Materials and Methods section.
Submitting Results
Results of the transgene screening should be reported
back to the TCMF using the Transgene Screening Results
form (see page 12). Failure to report results within 45 days
of receiving the tail samples will result in the forfeiture of
your right to a free re-injection in the event that only 0-2
founder mice can be detected.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 37
Housing Arrangement
Tails samples and mice should be received at the same
time by the investigator or the project scientist. We can no
longer hold the mice while the samples are being screened
for the presence of the transgene. The P.I.’s should prearrange
for space availability in their assigned facility.
Appendix
Purification of DNA for Microinjection
1. Recombinant plasmid should be purified by CsCl
gradient (for details see Molecular Cloning: A
laboratory manual, 2nd ed. Sambrook, et al. 1990).
The insert is then released with the appropriate
restriction enzymes. It is critical to remove all vector
sequences, as such sequences can significantly alter
the expression of transgenes (mechanism unclear).
2. Separate the insert of interest from the vector on an
agarose gel run in Tris/Acetate/EDTA (not Tris/ Borate/
EDTA) buffer.
3. Excise the gel slice containing the gene fragment of
interest and electroelute the DNA fragment.
4. Recover the fragment by ethanol precipitation, and
then pass it through an ion exchange column (e.g.,
Schleicher & Shuell Elutip columns) according to the
manufacturer’s instructions.
5. Ethanol precipitate the DNA and redissolve it in
injection buffer (10 mM Tris/0.1 mM EDTA pH 7.5).
This buffer must be prepared with Milli-Q water to
maintain zygote viability. We can provide this type of
water upon request.
6. Estimate the DNA concentration by comparing the
ethidium bromide staining of a sample run on an
agarose gel next to a standard of known concentration
such as • DNA cut with Hind III.
7. Adjust the DNA concentration to 5 ng/µl with injection
buffer.
Alternate Procedure
1. After separating the fragment on agarose gel run in
TAE, excise the fragment of interest from the gel.
2. Process the gel fragment through Geneclean II kit (Bio
101, Inc.) or Qiaex gel extraction kit (Qiagen, Inc.)
according to the manufacturer’s instructions.
3. Ethanol-precipitate the recovered DNA and resuspend
in Elutip buffer
4. Pass through an Elutip Column (Schleicher & Shuell).
5. Ethanol precipitate the recovered DNA and resuspend
in injection buffer (10 mM Tris/0.1 mM EDTA, pH 7.5
prepared with Milli-Q water).
6. Estimate the DNA concentration by comparing the
ethidium bromide staining of a sample run on an
agarose gel next to a standard of known
concentration.
7. Adjust the DNA concentration to 5 ng/µl, using
injection buffer.
Notes
_ For ethanol precipitation of a sample, add 1/10 volume
of 3 M NaAc and 2-2.5 vol. ethanol.
_ Use 1:100 dilution of EtBr (5 mg/ml) for gel staining.
Visualize the DNA band with long-wave UV light to
avoid DNA damage.
_ It is strongly recommended that powder-free gloves be
used when handling the DNA preparation. Powdered
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 38
gloves are most often associated with the presence of
particulate matter that gets lodged at the tip of the
injection needle and renders it useless.
Protocol for Isolating High-Molecular-Weight DNA
from Mouse Tails
(From Manipulating The Mouse Embryo; Hogan et al. Cold
Spring Harbor, 1986).
Day 1
1. Cut 0.5-1.0 cm of tail and place in a 1.5-ml microfuge
tube (tails can be frozen at -20 •C prior to extraction).
These tail biopsies will be performed by the Core.
2. Add to the tube 0.7 ml of 50 mM Tris (pH 8.0), 100 mM
EDTA, 0.5% SDS. Add 35 µl of a fresh 10 mg/ml
solution of Proteinase K.
3. Incubate at 55 •C overnight on a gently rocking
platform.
Day 2
4. Next morning, remove tubes from 55 •C. Add 0.7 ml of
phenol (equilibrated with Tris - pH 8.0). Close tube
and shake vigorously for 3 minutes, so that phases
mix completely.
5. Centrifuge in a microfuge for 3 minutes. Phases will
separate.
6. Transfer upper aqueous phase to a fresh tube, being
careful not to pick up phenol or material at interface.
7. Add 0.7 ml of phenol/chloroform (1:1), shake
vigorously for 2 minutes and centrifuge for 2 minutes.
8. Again remove aqueous phase, avoiding interface, and
transfer to a 1.5-ml tube.
9. Add 70 µl of 3 M sodium acetate / pH 6.0 (i.e., 1/10
volume), and 0.7 ml of 100% ethanol at room
temperature. Shake to mix thoroughly. DNA should
immediately form a stringy precipitate. Sodium
acetate with a pH lower than 6.0 will cause the EDTA
to precipitate.
10. Spin in microfuge for 30 seconds to pellet DNA.
Remove and discard as much ethanol supernatant as
possible.
11. Add 1 ml of 70% ethanol (room temperature) to tube,
and vortex or shake vigorously to wash DNA. This
step is essential to remove traces of SDS and phenol.
12. Centrifuge in microfuge for 1 minute at room
temperature. Remove as much ethanol supernatant
as possible. Dry DNA briefly in vacuo.
13. Add 0.1 ml of 10 mM Tris (pH 8.0)/1 mM EDTA to tube.
Leave at room temperature overnight to dissolve. If
necessary, DNA can be dissolved more quickly by
heating for 5-10 minutes at 65 •C. The DNA should
have an A260/A280 of > 1.7, and the concentration
should be calculated using: 50 µg/ml = 1.0 OD260. Use
10 µg of each DNA preparation for Southern blot
analysis.
14. DNA prepared in this manner will contain substantial
amounts of RNA, but this does not interfere with
restriction enzyme digestion or Southern blot analysis.
When performing restriction enzyme digestion, add 5
µg of DNase-free RNase A to each sample along with
the restriction enzyme. Digestion with certain
restriction enzymes may also be aided by adding 4
mM spermidine to the reaction.
15. Make sure you include the appropriate positive and
negative hybridization controls for your Southern
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 39
analysis.
Preparation of BAC DNA for microinjection
Courtesy of Mike Thornton from the laboratory of Dr.
Mortimer Poncz, Professor of Pediatrics at the Children’s
Hospital of Philadelphia
1. Recombinant BAC plasmids should be purified using
preferably the QIAGEN Large Construct Kit. This will
yield at least 100 µg of pure plasmid free from the
Genomic DNA contamination usually associated with
BAC preps. CsCl gradient can be used alternatively
(for details see Molecular Cloning: A laboratory
manual., 2nd ed. Sambrook, et al 1990) with the
following guidelines: a) After adding Solution II and/or
III invert very gently about 8-10 times to ensure no
genomic DNA is sheared, b) During all steps
throughout this protocol use sterile wide bore pipettes,
and minimize the amount of pipetting. c) Use a needle
of size 18 gauge or wider to remove your band after
ultra-centrifugation d) when you remove your band be
absolutely sure not to remove the genomic DNA band
that typically appears above the plasmid band. Once
you have precipitated your DNA, the insert is then
released with the appropriate restriction enzymes.
2. Separate the insert of interest from the vector
sequence on a Seaplaque GTG(low melting) agarose
gel run in 0.5-1X TBE (Field Inversion Gel
Electrophoresis-FIGE Vs Standard electrophoresis
respectively).
3. Preparation of the gel
Reagents:
_Baxter’s Sterile Water _TRIS-Base
_Filter unit and receiver _Boric Acid
_1000 ml clean glass bottles _EDTA-4Na
_Nalgene 1000 ml sterilization
3a. 108g of Tris, 55g of Boric acid and 80ml of 0.5M
EDTA-4Na (prepared with Millipore Water) are mixed
by shaking in a sterile Nalgene receiver with 1000ml of
Baxter’s water to prepare 10X TBE. This solution is
then passed though a 0.2µm Nalgene 1000 ml filter
unit.
3b. 50-100ml of 10X TBE (above) and 900-950ml of
Baxter’s water are mixed and passed directly through
a second 1000 ml filter unit and the resulting
0.5X(FIGE) or 1X (standard) TBE solution is
transferred to a very clean 1L glass bottle for
autoclaving.
3c. 100ml of 0.5 or 1X TBE is then added using an
autoclaved glass graduated cylinder into a 500ml
autoclaved flask, containing no greater than 1 %(w/v)
Seaplaque GTG agarose. After heating make sure the
volume is still 100ml and add 0.1- 0.2µl of Ethidium
bromide, 10mg/ml stock (50-100 kb insert or < 50 kb
insert respectively)
3d. Gel trays, combs and Electrophoresis chambers are
washed with antibacterial soap and rinsed in millipore
water before pouring or running gels. Appropriate care
should be taken to keep the gels, gel trays chambers,
etc., as sterile as possible. Using autoclaved pieces of
aluminum foil to cover and protect items helps.
3e. Run molecular weight markers on both sides of the gel
and also run expendable sample lanes on each side of
the gel. The center lanes will contain the “real sample
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 40
“ to be isolated. Avoid exposing the center lanes to any
light normal or UV. The sample and marker lanes on
the sides can be used to monitor the progress of the
run.
4. Excise the appropriate sized insert band from the gel.
Supplies:
_UV Box
_Sterile Aluminum foil strips 10.5 cm X 7cm
_New- sterile unused Scalpels
4a. Clean the surface of the UV box with Sterile Millipore
water and/or 70% ethanol, air dry or dry using
sterilized paper towels.
4b. Place two sterile aluminum foil strips on top of each
other and place on the UV box.
4c. Place your gel (as little light as necessary in the room
should be present) on the aluminum strips such that
the marker lanes and sample lanes on both sides can
be exposed to the UV light whereas the center lanes
are on top of the foil protected from the UV light below.
4d. Using the marker and sample lanes on both sides as a
guide place a clean sterile ruler under the desired
band and use a scalpel to cut across the gel under the
band, place the ruler above the band, and repeat. Cut
away any excess gel on the sides of the desired
sample region. Cut the gel slice into 6-8 pieces of
equal size and place all slices into a 50-ml conical
tube. Add 45ml of Baxter’s EQ buffer (below)
5. Equilibrate the gel slices in Baxter’s EQ buffer in 2
exchanges over 1-2 hours.
Remove all buffer using a vacuum and sterile Pasteur
pipettes.
Place1-2 gel slices into a single sterile Eppendorf tube
(4-8 tubes total) and melt the gel slices at 68 C for 10
minutes then place tubes in a 42 C water bath for five
minutes to equilibrate.
Baxter’s Buffer:
Final Stock
10mM Tris pH 7.5 ...... 5 ml of 1M Tris pH 7.5
250µM EDTA ............ 250 µl of 0.5M EDTA pH 8.0
100mM NaCl ............. 10 ml of 5M NaCl
Baxter’s water ........... ~484 ml
Stock solutions used to make above dilutions should
only use Millipore water and is filter sterilized before
use. Add all components directly into 500 ml Nalgene
filter sterilization unit. Pour filtered buffer in to a very
clean glass bottle and autoclave.
6. Digest the agarose slices
Add 10 µl of beta agarase enzyme (New England
Biolabs) to every 500µl of melted gel slice, mix (pipette
with wide pore sterile tips) and incubate overnight at
42 C. The next day place tubes back at 68 C for 10
minutes and then 42 C for five minutes add 2µl more
of beta agarase and incubate for an additional 1-2
hours.
Centrifuge tubes for 10 minutes full speed to get rid of
any remaining debris or undigested agarose.
7. Concentrate the insert DNA
Carefully transfer your sample from each Eppendorf
tube into a Millipore Ultrafree - 4ml Centrifugal filter,
specifically the Biomax 50k size exclusion type
(Catalog # UFV45010B) This filter needs to be
prewashed with sterile water or Baxter’s EQ buffer to
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 41
get rid of the glycine preservative found on all new
filters. Be careful not to transfer any undigested
agarose or debris at the bottom over to the centrifugal
filter. Centrifuge the sample for 5 minutes at 5001000g. Your initial volume should fall between 3-4ml
before centrifugation and drop no lower than 500 µl
after spinning. The final solution has the same initial
salt concentration but increased DNA concentration. It
is advisable to keep the flow-thru as a precaution. You
can optionally add 3ml of T.E. buffer (10mM Tris pH
7.5 0.1 mM EDTA-4Na pH 8.0) prepared with Sigma
Water for embryo Transfer (Catalog #W-1503) This will
further dilute any proteins and salts remaining.
Centrifuge again at 500g for 5 minutes or until the
volume reaches 500 µl. Let the sample sit for one
hour, than pipette gently and transfer to an Eppendorf
tube. Filter the sample by placing it into a 1 cc syringe
and passing it though a 0.2µM Acrodisc filter (Gelman
Sciences Ann Arbor Michigan) This filter is specially
designed to not stick to DNA.
7a. Drop dialyze sample
Place a Millipore 0.025µm VS filter (catalog# VSWP
025 00) on to 10ml of T.E. buffer (10mM Tris pH 7.5
0.1 mM EDTA-4Na pH 8.0) prepared with Sigma Water
for embryo Transfer(Catalog #W-1503). Place a 50 µl
drop of your sample in the center of the filter and leave
overnight. The next day remove the DNA drop and
place in an Eppendorf tube. Determine the
concentration of the sample and dilute in filtered T.E.,
to reach a final concentration of about 2-3ng/µl.
Microinject the DNA within 1-2 days
Optional: To get rid of excess ethidium and for longterm
storage use protocol below.
7b. Ethanol precipitate your sample
After acrodisc filtering (step 6 above) - Add 1/10th the
volume of sample of 3M NaOAc(autoclaved and filter
sterilized( 0.2µM) and prepared with Millipore water)
then add 2X volume filtered( 0.2µM ) 98% Ethanol
place at -20 C until ready to use. This storage is good
for several months. Centrifuge DNA and wash pellet in
filtered 70 % ethanol, dry and add 200 µl of filtered
(0.2µM) T.E. buffer(10mM Tris pH 7.5 0.1 mM EDTA4Na pH 8.0) prepared with Sigma Water for embryo
Transfer. Drop dialyze the sample as above, and
determine the concentration of the sample and dilute
in filtered T.E., to reach a final concentration of about
2-3ng/µl. Microinject the DNA within 1-2 days
Preparation of HNF3• YAC DNA for Microinjection
Courtesy of Newman Sund and Dr. Klaus H. Kaestner,
Department of Genetics, University of Pennsylvania School
of Medicine
For additional reference: Schedl, A. et al. (1993) Nucleic
Acids Research. 21: 4783-4787.
1. Equilibrate 8 yeast plugs (Large Scale Yeast Plug
Prep) 1x 10', 1x 30', 1x 1hr in 50 ml/wash of 0.40x TBE
(BioRad 10xTBE #161-0733). Equilibrate “PFGE
markerI-Lambda ladder”(BMB #1378961) at this time
as well. It is recommended that you make 3.0 liters of
0.40xTBE for plug washes, gel and running buffer.
2. Load plugs into 4 cm. slot of 1.0 % BioRad PF-certified
agarose gel in 0.40xTBE. Make a 200 ml gel using the
preparative comb. Plugs are positioned vertically,
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 42
tightly next to each other, and flanked by • ladder.
Seal the plugs in the gel with 1.5% SeaPlaque Low
Melting Point (LMP) agarose.
Note
_ The original protocol uses a 1.0% SeaPlaque (FMC)
LMP agarose PF gel. Non-LMP agarose is
recommended for use because the 1% LMP agarose
gel is fragile and difficult to work with in steps 5 - 7.
When excising the YAC DNA in step 8 using the nonLMP agarose, make sure not to excise the 1% gel with
the 4% gel. The non-LMP agarose will not melt at
68 •C in step 10. Using the non-LMP agarose may
affect the migration of the YAC DNA into the 4% LMP
gel and affect the yield.
3. Run gel as optimized previously. For HNF3• YAC: 515 s. switch, 20 hr. run, 6V/cm, 120 • angle, 14 •C, 2.4
L of 0.40xTBE. You want the current to run 120-145
amps during the run, closer to 120A at the beginning of
the run.
4. After the run, do NOT stain the entire gel. Instead, cut
off both sides of the gel (• ladder plus half of one lane
each - see Figure 1) and stain the marker gel pieces in
0.5 µg/ml Ethidium Bromide (EtBr) (20 µL 10 mg/mL
EtBr stock in 400 mL dH20) for 30 min. rocking. While
marker gel pieces are staining, soak rest of the
unstained gel in 1 x TAE buffer (Maniatis). It is
recommended that 4L of 1 x TAE is made for all the
washes and the running buffer. Observe marker gel
pieces under UV and take picture of gel with ruler
aligned with the top of the gel. Note the position of the
YAC band as well as two reference chromosomes.
5. Now excise the YAC band and the two individual
reference chromosomes from the unstained, pulse
field gel (cut out ±2.5mm). Stain the rest of the gel to
confirm that you cut out the correct band.
6. Equilibrate gel slices in 1 x TAE buffer 1x 1hr, 1x 2hr
rocking very gently.
7. Position gel slices in a minigel tray with the YAC slice
in the middle (see Figure 2). The gel slices should be
positioned lengthwise, such that the DNA will migrate
at a 90 • angle to the original PFGE run. Seal the sides
of the gel pieces onto the tray with 4% LMP-agarose
(NuSieve GTG, FMC) in 1x TAE to keep the gel pieces
precisely positioned. Prepare the 4% LMP gel
beforehand and keep at 48 •C. LMP agarose foams
and boils over easily so watch it carefully as it heats.
Cast 4% LMP-agarose in 1 x TAE around the slices,
barely covering them (~180-200 mL). Make sure that
the surface is level when casting and running the gel.
Keep some tape on tray ends to prevent the gel from
sliding off during the run. Electrophorese for 16 hours
at 60V (2.2V/cm) while recirculating the buffer.
8. Excise one entire reference chromosome plus at least
3 cm into the 4% gel where the DNA has migrated into
and stain the gel piece in EtBr, as above. If there is
still a significant amount of DNA in the 1% gel,
electrophorese longer at 110V (4V/cm) for an
additional 1-2hr and stain the other reference
chromosome. Estimate the position of the YAC based
on the position/location of the reference chromosome
DNA and cut out the minimal YAC band. Place into
tared µfuge tube and determine volume of the block. 1
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 43
mg=1µL. If necessary, cut the agarose block into
smaller pieces such that each block is approximately
100-200 mg. This aids melting in step 10 and
potentially concentrates the DNA in some aliquots.
Stain the rest of the YAC slice to confirm you cut it out.
(see Figure 3)
9. Equilibrate gel block 1x 10', 2x 1hr. in 15 ml/wash of 1
x TAE, 100 mM NaCl, 30 µM Spermine, 70 µM
Spermidine.
10. Melt agarose block for 20 min at 68 •C, stirring the
solution after 10 minutes with a sterile pipette tip. Cut
the gel block into smaller pieces to aid melting. Do not
incubate agarose at temperatures greater than 70 •C,
as it will denature the DNA. Once melted, transfer tube
immediately into 42 •C water bath and equilibrate the
FIGURE 1 FIGURE 2 FIGURE 3
molten agarose for 10 minutes at 42 • C. GELase
loses activity at temperatures greater than 45 •C. Add
5 units (1U/µL) GELase (Epicentre) per 100 mg
agarose and incubate at 42 •C for 2 hours. While in
heat block, stir molten agarose a couple of times with
pipette to ensure even distribution of GELase. After 2
hours, place test tube on ice for 10 minute to test if
digest is complete. If gel reforms, repeat step 10 with
more GELase.
11. Pulse briefly at 3000 rpm for 10 sec. Dialyze against
YAC micro-injection buffer for 3 hours. For this, place
a Dialysis Membrane (0.05 microns, type VM,
Millipore) on top of 50 ml of YAC micro-injection buffer
in a Petri-dish. Pipette YAC DNA solution carefully on
top of the membrane using a cut-off blue tip, dialyze
for 3 hours, and transfer YAC DNA to a fresh µfuge
tube. Save a 1 ml aliquot of YAC micro-injection buffer
for dilution of YAC DNA in step 13.
12. Estimate YAC DNA amount by PFGE (load 20 µL)
against known standards (10, 20, 40, and 80 ng).
Include positive control and • marker as well. Can
stop run at 18.5 hours to see plasmid stds.
Notes for handling YAC DNA
_ Always tap mfuge tube to mix purified YAC DNA prior
to taking off an aliquot.
_ Never vortex or pipette up and down to mix. This will
shear the linear YAC DNA.
_ Use a cut-off pipette tip when you need to take an
aliquot of YAC DNA.
_ Store YAC DNA at 4 •C until ready for use.
13. Dilute YAC DNA to 0.3-2.0 ng/µL. You have to dilute at
least 2:1 with YAC micro-injection buffer: YAC DNA for
micro-injection. Therefore, the YAC DNA must be at
minimum 0.9 ng/µL when quantitated.
YAC micro-injection buffer
(10 mM Tris/Cl pH 7.5, 0.1mM EDTA pH 8.0, 100 mM NaCl,
30 µM Spermine, 70 µM Spermidine)
For 200 mL
1M Tris pH 7.5 2.0 mL
5M NaCl 4.0 mL
0.5M EDTA 40 µL
0.3M Spermine (1:10,000 diln) 20 µl
0.7M Spermidine 20 µL
MQ ddH20 194 mL
1xTAE, 100 mM NaCl, 30 µM Spermine, 70 µM
Spermidine
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 44
For 200 mL
50xTAE 4.0 mL
5M NaCl 4.0 mL
0.3M Spermine 20 µL
0.7M Spermidine 20 µL
MQ dd H20 192 mL
0.3M Spermine (348 g/mol)
For 1 ml: 0.104g in 1.0 ml ddH20
0.7M Spermidine (255 g/mol)
For 1 ml: 0.179 g in 1.0 ml ddH20
Large Scale Preparation of Yeast Plugs
1. Inoculate 100 ml medium (YPAD or selective medium)
with 0.2 to 1 ml of a stationary culture. Shake at 30 •C
for 1 to 2 days. Density should be at least 5 x 107
cells/ml.
2. Pellet cells in 50 ml Falcon tubes at 3000 rpm for 5
min.
3. Resuspend pellets in 40 ml (total) 50 mM EDTA, pH 8.
Spin as above
4. Weigh a Falcon tube. Resuspend cells in Solution 1
and pellet in Falcon tube.
5. Determine the weight of the pellet. Assuming 1g =
1ml, you now know the volume of the pellet. Add 0.5
volume Enzyme Solution and warm to 37 •C.
6. Now add equal volume of pre-warmed (40 •C) Agarose
Solution. Suspend the cells using a cut-off blue tip.
Pipett into 8- microliter block forms.
7. Place on ice for 10 min. Gently remove plugs and
incubate for 2 hours at 37 •C in 5 ml per plug of
Solution 2.
8. Transfer to LDS at 37 •C. After one hour, transfer to
fresh LDS and incubate (gentle shaking) at 37 •C
overnight.
9. Next day, wash plugs for two hours in 20% NDS at
room temp. Store plugs indefinitely at RT.
10. Before use, plugs have to be equilibrated in restriction
buffer or TE.
Solution 1
1 M Sorbitol
20 mM EDTA, pH 8
14 mM b-mercaptoethanol (•-ME)
Solution 2
1 M Sorbitol
20 mM EDTA, pH 8
14 mM b-ME
10 mM Tris/HCl pH 7.5
2 mg/ml Zymolase-20T (ICN)
(make up fresh)
Enzyme solution
6 mg/ml zymolase-20T (ICN) in Solution 1
100% NDS
350 ml H2O, 93 g EDTA
0.6 g Tris base.
1. Add NaOH pellets until pH > 8.0.
2. Dissolve 5 g N-Lauryl-Sarcosine in 50 ml H2O and
add to above.
3. PH to 9.5 with NaOH, adjust volume to 500 ml.
4. Store at RT.
2001 GENETICS CORE FACILITIES CATALOG
36
Agarose Solution
1 M Sorbitol
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 45
20 mM EDTA, pH 8
2% Sea Plaque GTG Agarose (FMC)
Once melted, cool to 40 C and add •-ME to 14 mM
LDS
100 mM EDTA pH 8.0
10 mM Tris/Cl pH 8
1% Lithiumdodecyl sulfate
Preparation of ES Cells
(Procedure to prepare ES cells for blastocyst
injection on Friday)
Courtesy of Joanne Thorvaldsen from the Laboratory of Dr.
Marisa Bartolomei, Department of Cell and Developmental
Biology, University of Pennsylvania School of Medicine.
On Monday
1. Seed ES cells onto one well of 24-well plate
1a. Plate mouse embryonic feeder cells (MEFs) onto
multiple wells of 24-well gelatinized plate.
1b. After MEFs have attached to plate (2+ hrs), thaw ES
cells and seed into one well of the prepared 24-well
plate.
On Wednesday
2. Split cells as necessary, onto wells of the prepared 24well plate.
2a. If well is confluent (50-60%), split to various densities
into three wells of the 24-well plate e.g., approximately
1:2, 3:10 and 1:5 into each well. (See Note
below.) The trypsinization method described below
works well to ensure single-cell suspension.
2b. If colonies are few or are not growing well, plate
trypsinized cells onto one well. In addition, consider
plating out an additional vial of ES cells onto another
well, to ensure some population of ES cells will be
ready for Friday.
On Thursday or Friday morning
3. Confirm with Jean Richa that he is prepared for your
ES cells (Laboratory Telephone: 215/573-3023, Office
Telephone: 215/898-6064).
On Friday
Note
_ The harvested cells need to be ready for injection
by noon.
_ Two separate wells of ES cells which vary in
confluence may be harvested simultaneously to
provide Dr. Richa with the most optimum cell density
required for the blastocyst injections.
4. Feed cells around 8:00am.
5. Trypsinize cells at around 10:20am.
5a. Wash twice with PBS
5b. Add trypsin (0.25% Trypsin, 1 mM EDTA; 0.2-0.3 ml/
well of 24well dish) and incubate for 5 minutes at 37oC.
5c. To disaggregate the cells, use a plugged Pasteur
pipette and pipette cells up and down 5X. Then add 1
ml ES media. With a plugged Pasteur pipette inserted
into a P-200 type narrow bore yellow tip, pipette entire
cell suspension up and down (15-20X).
Note
_ It is important to generate a single cell suspension
for blastocyst injections. Avoid foaming of the
medium!
6. Adsorb MEFs on gelatinized 6-well plate and collect
ES cells.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 46
6a. Transfer ES cell suspension to one well of gelatinized
6-well plate which contains 1-2 ml ES medium (final
volume 2-3 ml) .
6b. Leave plate in incubator for 1 hour. (The MEFs should
sit on the plate after one hour while the stem cells are
still in suspension.)
6c. Carefully transfer supernatant to 15 ml conical tube
and centrifuge for 5 minutes at 900 rpm (e.g. IEC HNSII
Centrifuge).
6d. Discard medium and resuspend the cells in appr. 0.5
ml ES cell medium. To a separate tube, add 5 ml ES
cell medium.
7. Deliver 15 ml tube(s) containing ES cells and tube with
5 ml ES cell medium to Jean Richa, Room 202A CRB.
(Tubes may be delivered on ice in a Styrofoam box.)
Basics for culturing ES cells:
_ Colonies should be compact with a smooth outline
(see ‘Manipulating the Mouse Embryo’, CSHL Press,
Ed. Brigid Hogan et al.(1994)).
_ Culture in the presence of LIF (1000U/ml, Gibco/BRL)
on mitotically inactivated mouse embryonic feeder
(MEF) cells that were plated onto gelatinized plates.
Use ES medium as recommended for the specific ES
cell-line in use.
_ Feed cells daily (and 2-3 hr before cells are
trypsinized).
_ Do not grow cells for more than 3 days (2 days
preferred) without splitting (1:3 to 1:6). Confluent/fast
growing cells will need to be split one day after
seeding/splitting.
_ To prepare gelatinized plates (or wells), cover plate
with 0.1% gelatin solution and leave plate in biosafety
cabinet for 2 hrs. Remove gelatin and allow gelatin to
dry for approximately 10 minutes. (Plates must be
completely dry before use or storage at 4o C.)
On Animal Husbandry
Since all animal maintenance and subsequent breeding of
transgenic mouse lines will be carried by the individual
investigator, a knowledge of basic mouse husbandry is
essential. Here are some basic guidelines and
suggestions.
_ Mice are born 19-20 days after vaginal plug detection
(i.e., successful mating).
_ If neonates do not show white stomach (milk) 24 hours
after birth, suspect a bad mother; foster mother
(lactating female) should be used or pups will die.
_ Litters should be weaned at 3 weeks after birth.
_ Sexual maturity of females: approximately 6 weeks;
that of males; approximately 8 weeks. Maturing males
should be housed individually as they tend to become
aggressive with one another.
_ Watch for water bottle leaks; neglected wet cages can
result in the death of animals.
_ For animal health problems in your colony or more
specialized information, please contact the attending
veterinarian of the University Laboratory Animal
Resources.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 47
CELL CENTER
STOCKROOM
Maurice Fluellen, Operations Manager
215/898-2069
fluellen@mail.med.upenn.edu
Ian Blake, Clerk
Allen Shinn, Clerk
Sean-Paul Fenton, Clerk
Adam Mancini, Clerk
Phineas Walsh, Clerk
Val Riordan, Clerk
215/898-2069
Stockroom Hours:
Monday - Friday, 9:00 a.m. - 5:00 p.m.
Closed 1:00 p.m. - 2:15 p.m. every day
Location:
D1 Richards Building
Mailing Address:
502 Clinical Research Building
415 Curie Boulevard
Philadelphia, PA 19104-6145
Telephone: 215/898-2069
Telephone Ordering System: 215/898-5306
Fax: 215/573-3263
E-mail (General Information): cellctr@mail.med.upenn.edu
E-mail (Oligo Orders): ccoligo@mail.med.upenn.edu
Free special ordering service - pay list price or less • Molecular biology and tissue culture products at
discount prices • Free delivery to your lab • Immediate pickup availability • Over 1400 items in stock •
Products from 35 different vendors • Special orders of non-stocked items available from 20 vendors •
Most special orders available next day • No shipping fees or handling fees, ever • Oligo Nucleotides
available, see page 35 • 24 hour ordering via phone system or CellNet
U N I V E R S I T Y O F P E N N S Y LVA N I A
D E PA R T M E N T O F G E N E T I C S
Cell Center Stockroom
Opening an Account
1. An account must be established for each Principal
Investigator before products or services may be
ordered. Once all the required information (see below)
has been submitted to the Stockroom, the account will
be available for use within one business day.
2. Opening and maintaining an account requires no fees
or dues. Purchases are billed on a monthly basis.
3. Three different forms are required to set up a new
account. a single Lab Account Information Form, one
or more Charge Account Information Forms, and
several Lab Member Information Forms. These forms
can be found beginning on page xii in the Appendix.
After these forms are submitted, each Lab Member will
receive an e-mail containing his or her User ID and a
randomly generated password to access Stockroom
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 48
ordering systems. Forms may be submitted in person
or by fax.
Lab Account Information Form — this contains basic
contact information for each lab, as well as the
Business Administrator’s contact information for billing.
Only one copy of this form should be submitted for
each principal investigator or lab.
Charge Account Information Form — this contains
basic FinMIS and grant information. A separate copy
of this form should be submitted for every funding
source that you wish to use for Stockroom purchases.
You must specify a short nickname (two to four words)
for the Charge Account, to allow all of us to reference it
easily. You may limit access to each Charge Account
to only certain Lab Members. You may also specify a
Charge Account Phone Code for each Charge
Account, to allow you to use any or all of your funding
sources with our phone ordering system.
Lab Member Information Form — a separate copy of
this form should be submitted for each Lab Member
who will be placing orders, this includes special orders
and oligonucleotide synthesis. It allows you to specify
which Charge Accounts should be used by each Lab
Member, as well as a unique user code for our phone
system. The e-mail address and phone number are
required, and each Lab Member receives his or her
own unique User ID and password.
4. To change account information:
It is very important that account information be kept
current. Please use the Lab Account Information
Form (page xiii) to update delivery locations and other
basic laboratory contact information.
To add a new grant or other funding source to your
Lab Account, you only need to submit a single Charge
Account Information Form, where you may specify
which Lab Members should have access to the new
funds. New Charge Accounts will be available for
purchases within one business day of submission of
the form.
To obtain access for a new Lab Member within your
lab, you only need to submit a single Lab Member
Information Form, where you may specify which
Charge Accounts the new lab member should use.
New Lab Members will be given access within one
business day of submission of the form.
It is essential that the Business Administrator inform
the Stockroom when a Charge Account has expired or
when a Lab Member has left the lab. This will avoid
unauthorized use of the account. Charge Accounts
and Lab Members can be de-activated by the Principal
Investigator or Business Adminstrator via fax or e-mail.
All de-activations are effective immediately or as soon
as possible.
The Stockroom cannot be held responsible for unauthorized
use of your account if you have not kept your
account information current. It is your responsibility to
ensure that departed Lab Members are removed from your
Lab Account and to keep all passwords secure.
The Stockroom will not be responsible for any delays
due to incomplete forms.
How to Place an Order
1. You can place your order at the Cell Center between
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 49
the hours of 9:00 a.m. and 5:00 p.m., Monday-Friday.
Please note the Cell Center is closed daily from
1:00 p.m. to 2:15 p.m..
2. Automated telephone ordering (898-5306): This
system allows order entry through a touch-tone
telephone and is available 24 hours a day.
NOTE: Please listen for the machine’s acknowledgment
that the item number was entered before
continuing with your order. Also, if you have a multiline
Eagle or Extrom digital phone, press *# before
starting to enter the codes to simulate a touch-tone
phone.
The telephone ordering system uses numeric codes to
identify specific Charge Accounts, Charge Account
passwords, Lab Members and products. You are free
to choose codes that would be easy to remember, but
the first digit of a number code cannot be “0”. The
Charge Account code and password code can range
from 4-9 digits, but Lab Member codes must be 4
digits in length.
NOTE: Orders placed through the telephone ordering
system are not immediately available to
be filled by a staff member. If you have an emergency
order, please come to the Stockroom, and a staff
member will fill your order except between 1:00 and
2:15 p.m.
3. Only Special Orders may be faxed. All other orders
should be placed either by the Stockroom kiosks, CellNet, or via the
phone ordering system. Special orders should be
faxed using the form located the Appendix, or entered
at the Stockroom.
Special orders are those items which are not stocked
at the Stockroom. Any item that is not stocked at the
Stockroom may be special ordered. The following
vendors allow special orders and most items are
available the next day. Specific information about the
availability of special orders can be found in each
vendor’s section of the catalog. The Stockroom
cannot be responsible for vendor backorders, shipping
delays or other circumstances beyond our control.
SPECIAL ORDER VENDORS
Amersham Biosciences
Applied Biosystems
Roche Molecular Biochemicals
BIO-RAD
Clontech
Denville Scientific Products / Q-Biogene / TKR
Invitrogen (Life Technologies)
Perkin Elmer Life Sciences
New England Biolabs
QIAGEN
Roche Molecular Biosystems
Sigma
Stratagene
If there are items or vendors that you frequently use that
are not currently stocked by the Stockroom, please notify
us. We will be happy to consider such additions.
All prices and information in this catalog are subject to
change.
To Pick Up Your Order
Customers may specify each order to be delivered or for
pickup at the Stockroom. Orders placed at the Stockroom
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 50
may be picked up immediately. For orders placed remotely,
orders submitted before 1:00 p.m. may be picked
up at any time after 2:15 p.m. Other orders may be picked
up at any time starting at 9:00 a.m. the next day.
Please remember that the Stockroom is closed between
1:00 p.m. and 2:15 p.m. every day.
Please double-check your order before leaving the
Stockroom, or before you sign for your delivery, to make
sure that it is complete and correct. Keep the receipt for
your records. Occasionally, items on your receipt may be
marked “o/s” (temporarily out of stock) or “b/o”
(backordered by the vendor). The Stockroom has no
control over this situation. It is our policy to notify the user
by telephone in this situation and keep a copy of the
receipt. As soon as the product becomes available to the
Stockroom, we will either deliver it or notify the customer.
We cannot exchange or refund opened packages.
Delivery
Morning deliveries (10:00 a.m. to 1:00 p.m.) include all
orders placed before 8:00 a.m. for the following buildings:
Route A Route B
Chemistry†‡ Abramson
CRB BRBI
Dulles BRBII/III
Founders Kaplan
Gates Mudd
John Morgan New Bolton Center†‡
Johnson Pavilion Old Vet†
Maloney Rosenthal†
Penn Tower HUP†
Ravdin Wistar†
Silverstein
Stemmler
Towne†‡
Afternoon deliveries (after 2:30 p.m.) include all orders
placed before 1:00 p.m. for the following buildings:
Route A Route B Route C
CRB Abramson Anatomy/Chemistry
John Morgan BRB Goddard
Johnson Pavilion BRB II/III Richards
Stemmler Wistar† Leidy
Levy†
VHUP†
There are never any charges for delivery, shipping or
handling for any Stockroom order.
Labs are limited to ten (10) bottles of media per day.
Special arrangements may be made with the Stockroom for
larger orders. Please call 898-2069 for more information.
† deliveries to these buildings are limited to 5 bottles of
media per lab per day and may be limited or suspended
during inclement weather at the Stockroom’s discretion.
‡ Tuesday/Thursday morning only or by special request.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 51
OLIGONUCLEOTIDE
SYNTHESIS
Invitrogen (Life Technologies)
Technical support: 800/828-6686
Internet: http://www.lifetech.com
Pricing
• 10 nmole scale (>2 OD) - $0.25 per base
• 50 nmole scale (>2 OD) - $0.41 per base
• 200 nmole scale (>10 OD) - $0.90 per base
• 1000 nmole scale (>50 OD) - $1.95 per base
• Setup fee: No charge (Desalted).
• Setup charges:
Cartridge 50/200nm $24.00
Cartridge 1000nm $30.00
HPLC 200nm $52.75
HPLC 1000nm $90.00
PAGE 200nm $65.00
PAGE 1000nm $75.00
• No delivery or shipping fees
• Delivery:
= 45 bases - 2nd business day;
> 60 bases, 5’ Phosphorylation, Iosine, Uracil or
Cartridge Purity - 3rd business day
> 60 bases, 1000 nmole scale, 5’ modifications
(except Phosphorylation) or
5’ Oligos - within 5 business days
HPLC and PAGE purity - within 6 business days
• Cell Center must receive order by 2:30p.m. (fax or email)
• Modifications:
5’Phosphorylation: 50/200nm - $25 1000 nm - $56
5’Biotin: 50/200 nm - $49 1000 nm - $110
Inosine: 10/50/200 nm - $8 1000 nm - $16
Mixed bases: N/C
Uracil: 10/50/200 nm - $7 1000 nm - $10
Technical Information
LTI Custom Primers are synthesized on a highly automated
computer controlled parallel array synthesizer using
standard phosphoramidite chemistry. Each order is double
entered into the system ensuring correct input. Coupling
efficiencies are monitored throughout synthesis on each
oligonucleotide by trityl analysis. Five levels of purity are
currently available: deprotected only; deprotected/desalted
by C18 spin-column chromatography; cartridge; HPLC and
PAGE. A comprehensive certificate of analysis accompanies
each oligonucleotide indicating quantity, molecular
weight, extinction coefficient, sequence, and melting
temperature.
Ordering Information
Place your order by:
FAX: use the appropriate forms located in the Appendix
and fax to 215/573-3263 by 2:30 p.m.
E-Mail: use the following format and send to
ccoligo@mail.med.upenn.edu by 2:30 p.m..
Invitrogen Procedure for Sending
Custom Oligo Orders Via E-mail
_ Please follow instruction exactly (no additional or
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 52
fewer spaces).
_ “Subject” line must indicate LTI OLIGO.
LTI
User Name ID
Password (non numerical)
Charge Account
Phone Number
To Be Delivered? (Y or N)
Date
*
Researcher Name
Primer Name
Sequence (All capitals please, limit 100 bases 5’ to 3’)
50, 200, or 1000 (nmole scale)
P, G, R, L, B, H, T, F or #
P if 5’ Phosphorylation
G if 5’ Fluorescein
R if 5’ Rhodamine
L if 5’ Primary Amine
B if 5’ Biotin
H if 5’ HEX
T if 5’ TET
F if 5’ FAM
# = none
D, S, O, P or H
D = Deprotected
S = de(S)alted
O = Cartridge
P = PAGE, H= HPLC
*
Next Primer (same format as above)
*
Last Primer (same format as above)
Sigma-Genosys
Technical support: 800/234-3693
Pricing
• 3OD Select - $0.30 per base
50 nmole scale - $0.70 per base
200 nmole scale - $1.30 per base
1000 nmole scale - $2.20 per base
• Setup fee: No charge (Desalted).
• Setup charges:
Cartridge 50/200nm $20.00
Cartridge 1000nm $32.20
HPLC 50/200nm $60.00
HPLC 1000nm $88.55
PAGE 50/200nm $60.00
PAGE 1000nm $88.55
• No delivery or shipping fees
• Delivery:
= 35 bases - 2nd business day;
> 35 bases - 3rd business day;
5’ modifications or
5’ Oligos - within 5 business days
HPLC and PAGE purity - within 5 business days
• Cell Center must receive order by 2:30p.m. (fax or email)
• Modifications:
5’Fluorescein: 50/200nm - $40.25 1000 nm - $80.50
5’Phosphorylation: 50/200nm - $20 1000 nm - $40.25
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 53
5’Biotin: 50/200 nm - $40.25 1000 nm - $80.50
Inosine: 50/200 nm - $6.45 1000 nm - $12.00
Mixed bases: N/C
Please call the Stockroom for more information regarding
Sigma-Genosys oligos.
Technical Information
Sigma-Genosys oligos are synthesized using our patented
AbacusTM platform which yields a higher coupling efficiency
than most competitor platforms. We offer 4 levels of
purification: desalt, reverse phase cartridge (RP1), PAGE
and reverse phase HPLC. Each and every oligo is
physically tested by gel electrophoresis and spectrophotometer
analysis. Additionally, each synthesis run is
statistically tested using MALDI-ToF mass spectrometry
analysis. At Sigma-Genosys, quality is not just a goal - it is
a guarantee. In addition to our stringent quality analysis,
you will receive a Quality Assurance Certificate that
includes quantitated yield, melting temperature, MW and
µg/OD with every oligo. You will also receive an additional
label that can be used in your records or on an additional
vial. All this at no extra charge.
Ordering Information
Place your order by:
FAX: use the appropriate forms located in the Appendix
and fax to 215/573-3263 by 2:30 p.m.
E-Mail: use the following format and send to
ccoligo@mail.med.upenn.edu by 2:30 p.m.
Sigma-Genosys Procedure for Sending
Custom Oligo Orders Via E-mail
_ Please follow instruction exactly (no additional or
fewer spaces).
_ “Subject” line must indicate GENOSYS OLIGO.
SIGMA GENOSYS
User Name ID
Password (non numerical)
Charge Account
Phone Number
To Be Delivered? (Y or N)
Date
*
Researcher Name
Oligo Name
Sequence (All capitals please, limit 110 bases 5’ to 3’;
3OD 10-35 bases)
3 OD Select, 50, 200, or 1000 (nmole scale)
DST, RP1, PAGE or HPLC
DST = Desalted
RP1 = Cartridge
PAGE
HPLC
NOTES (Define any special modifications)
*
Next Primer (same format as above)
*
Last Primer (same format as above)
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 54
PRIMERS & PROBES
Applied Biosystems
Technical Support: 800/345-5224
Internet: http://www.pebiosystems.com
Product Offering & Pricing
_ Flat Rate plus $3.50 Setup fee
_ No Delivery or Shipping Fees
_ Cell Center must receive order by 12:30p.m.
_ Desalted & Deprotected
_ 40nm & 200nm scale Prism Primers & Prism Primer
Pairs delivered in 4 days. Automatic purification
included. Labels available include: 6-FAM, HEX,
NED, or TET
_ 40nm & 200nm TaqMan Probes and Sequence
Detection Primers delivered together in 5 days. HPLC
purification included. Labels available include: 6FAM,VIC, or TET on 5’ end; TAMARA on 3’ end.
ABI Prism® Primers
450007 40nm 5’-Fluorescent-labeled oligo. Maximum
of 30 bases. 4000pmol purified.
Flat Rate: $78.00
450006 200nm 5’-Fluorescent-labeled oligo.Maximum
of 30 bases. 40,000 pmol purified.
Flat Rate: $125.00
450017 1um 5’-Fluorescent-labeled oligo.Maximum of
30 bases. 300,000pmol.
Flat Rate: $158.00
Purified yeild of 150,000pmol
Extra 2 days
HPLC Charge: $86.00
ABI Prism® Primer Pairs
450056 40nm Primer Pair (one 5’Fluorescent-labeled
& one unlabeled up to 30mers.) 4000pmol
each primer purified.
Flat Rate: $100.00
450096 40nm Individual Loci for ABI Linkage Mapping
Set 5cM Map. 4000pmol of each primer
purified.
Flat Rate: $52.50
450059 200nm Primer Pair (one 5’Fluorescent-labeled
& one unlabeled up to 30mers.) 40,000pmol
each primer purified.
Flat Rate: $157.00
450062 1um Primer Pair (one 5’Fluorescent-labeled &
one unlabeled up to 30mers.) 300,000pmol
each primer.
Flat Rate: $234.00
Extra 2 days
HPLC Charge: $86.00
Tailed Primer Pairs:
_Adding a 7 base sequence[primer tailing] on the reverse
primer. Same specifications per scale as above [F]
primer pairs.
4304979 40nm scale tailed Primer Pair
Flat Rate: $103.20
4304981 200nm scale tailed Primer Pair
Flat Rate: $159.10
4304982 1um scale tailed Primer Pair
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 55
Flat Rate: $236.50
TaqMan® Probes & Sequence Detection Primers
450025 40nm TaqMan Probe, 5000-6000pmol
Flat Rate: $290.00
4304970 40nm Sequence Detection Primer, 4000pmol
Flat Rate: $25/primer
450024 200nm TaqMan Probe, 15000-25000pmol
Flat Rate: $540.00
4304971 200nm Sequence Detection Primer, 80000pmol
Flat Rate: $35/primer
450003 1um TaqMan Probe, 50,000-100,000pmol
Flat Rate: $750.00
450005 1um Sequence Detection Primer, 300,000pmol
Flat Rate: $62/primer
Ordering Information
_Place your order by e-mail using the following format
and send by 2:30p.m. to
ccoligo@mail.med.upenn.edu
Subject Line must indicate ABI OLIGO.
ABI
User Name ID
Password (non numerical)
Charge Account
Phone Number
To Be Delivered? (Y or N)
Date
* Example:
Part# 450007
Sequence name oligo 1
Sequence NEDagtccgcgtcgatctcga
For labels please use the following nomenclature:
NED,HEX, FAM, TET, VIC, Biotin, TAMARA
For redundancies, use the following IUB Group Codes:
M=(AC); R=(AG); W=(AT); S=(CG); Y=(CT); K=(GT);
V=(AGC); H=(ACT); D=(AGT); B=(CGT); N=(AGTC).
Stocked Products - Product Class Listings ..................................................
Chemicals and Reagents • (CH)..........................................................................................
Competent Cells • (CE) ......................................................................................................
Film and Membranes • (FM) ..............................................................................................
Immunological Reagents • (IM) .........................................................................................
Miscellaneous Products • (MS) ..........................................................................................
Molecular Biology Kits • (KT) ...........................................................................................
Molecular Biology Products • (MB) ..................................................................................
Non-radioactive Kits and Reagents • (NR). .......................................................................
Other Enzymes • (OE) ........................................................................................................
PCR Reagents and Nucleotides • (PC) ...............................................................................
Precast Gels • (PG) ............................................................................................................
Protein & Molecular Weight Markers • (MR) ....................................................................
Purification Kits and Columns • (CO)................................................................................
Restriction Enzymes • (RE) ...............................................................................................
Sera • (SM) ........................................................................................................................
Tissue Culture Media and Reagents • (TC) .......................................................................
Stocked Products - Listed by Vendor ...........................................................
Amersham Biosciences • (AMR) .................……………..................................................
Applied Biosystems • (PEC) ............................................................................................
57
58
57
63
64
68
65
66
69
69
71
74
67
62
74
78
78
80
80
101
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 56
Bio-Rad Laboratories • (RAD) ........................................................................................
Biowhittaker Molecular Applications • (BMA) ................................................................
Cambrex Biosciences • (FMC) .........................................................................................
Cell Center Services • (CCS) .............................................................................................
Clontech Laboratories • (CLN) ..........................................................................................
Collaborative Biomedical Products • (COL) .....................................................................
Denville Scientific • (DEN) ...............................................................................................
Difco Laboratories • (DIF) .................................................................................................
Enzo Life Sceinces • (ENZ) ...............................................................................................
Epicentre Technologies • (EPI) .........................................................................................
Fisher Scientific • (FSH) ....................................................................................................
Fuji Film • (FUJ) ................................................................................................................
Gemini Bioproducts • (GEM) ............................................................................................
Hyclone Laboratories • (HYC) ..........................................................................................
IGEN International (Fisher Scientific) • (IGN) .................................................................
Invitrogen (Life Techologies) • (INV) ...............................................................................
Kodak • (KOD) .....................................................................................….........................
Marligen Biosciences • (MLG)...........................................................................................
Mediatech (Fisher Scientific) • (MED) ..............................................................................
Micron Separations Inc. (Fisher Scientific) • (MSI) ..........................................................
Millipore • (MIL) ................................................................................................................
National Diagnostics • (ND) ...............................................................................................
New England Biolabs • (NEB) ...........................................................................................
Perkin Elmer (New England Nuclear) • (NEN) ................................................................
Polaroid Film • (POL) .......................................................................................................
Promega • (PRO)....................................................................................…........................
Q-Biogene • (DEN) ............................................................................................................
Qiagen • (QIA) ..................................................................................................................
Rainin Instruments • (RAI) ...............................................................................................
Roche Diagnostics • (BMB) .........................……………………………………….........
Sigma-Aldrich • (SIG) ......................................................................................................
Stratagene • (STR) ..................................................................................…......................
TKR Bioproducts • (DEN) ................................................................................................
USB Corporation • (USB) ...............................................................................................
Index of Stocked Products ..........................................................................
106
85
86
86
87
87
88
89
89
89
89
91
91
91
91
91
97
97
97
98
98
99
99
101
102
103
88
105
107
82
107
108
88
109
109
STOCKED PRODUCTS
U N I V E R S I T Y O F P E N N S Y LVA N I A
D E PA R T M E N T O F G E N E T I C S
COMPETENT CELLS
.............................................................................................................. CE
Mfgr. Mfgr No
STR 230245
STR 230255
STR 230240
STR 230250
LTI 18263-012
LTI 18265-017
LTI 18290-015
Code
5428
5430
5427
5429
4118
4119
5423
Item Description
BL21-Codon Plus (DE3)-RIL Competent Cells
BL21-Codon Plus (DE3)-RP Competent Cells
BL21-Codon Plus-RIL Competent Cells
BL21-Codon Plus-RP Competent Cells
DH5alpha™ Library Efficiency Comp Cells
DH5alpha™ Subcloning Efficiency Comp Cells
ElectroMAX DH10B Efficiency Comp Cells
Unit
ea
ea
ea
ea
1 ml
2 ml
5x.1ml
CC Price
$
196.77
$
196.77
$
196.77
$
196.77
$
94.88
$
43.69
$
156.66
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 57
LTI
PRO
PRO
LTI
LTI
LTI
INV
LTI
INV
INV
INV
STR
STR
STR
STR
STR
STR
STR
11319-019
L2011
L2001
12034-013
18258-012
10268-019
C862003
12297-016
C2020-03
C4040-03
C3030-03
200152
200238
200315
200228
200314
200236
200150
5426
4246
4247
5458
5425
5474
4637
4622
4334
5445
4335
4550
4129
5418
4258
4548
4130
5517
ElectroMAX DH5alpha-E Efficiency Comp Cells
HB101 High Efficiency Competent Cells
JM109 High Efficiency Competent Cells
Max Efficiency DH5@T1 Competent Cells
MAX Efficiency DH5alpha Efficiency Comp Cells
Max Efficiency STBL2
One Shot MACH 1 Comp eColi (LTI Shelf)
One Shot® MAX Efficiency® DH5a®-T1®
OneShot™ INVaF' Competent Cells
OneShot™ TOP10 Competent Cells
OneShot™ TOP10F' Competent Cells
SURE® 2 Supercompetent Cells
SURE® Competent Cells
XL 10-Gold Ultracompetent Cells
XL-1 Blue Electroporation Competent Cells
XL10-Gold Ultracompetent Cells
XL-1-Blue Supercompetent Cells
XL-2 Blue Ultra Competent Cells
5x.1ml
1 ml
1 ml
1 ml (5 x 0.2ml)
5x.1ml
1 ML
20 rxn
20 x 50ul
21x50µl
21x50µl
21x50µl
ea
5x0.2ml
10 x 100µl
5x80µl
5x100ul
5x0.2ml
ea
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
154.63
58.83
67.61
123.50
157.71
148.43
235.31
243.98
243.61
238.08
235.31
156.21
152.30
194.93
161.25
129.39
146.57
183.82
CHEMICALS AND REAGENTS
.............................................................................................. CH
Mfgr. Mfgr No
Code Item Description
MED MT46-010-CM 1290
10X TAE
MED MT46011CM 1291
10X TBE
DEN CS-5585-28
3306
20% SDS Solution
MED MT46020CM 1292
20X SSC Buffer
LTI 10812-014
5453
2nd Strand Buffer
RAD 1610154
2392
30% Acrylamide/Bis 19:1
RAD 1610156
2393
30% Acrylamide/Bis 29:1
RAD 1610158
2394
30% Acrylamide/Bis 37.5:1
SIG A8456-100mg 5913
4-(2-Aminoethyl)Benzenesulfonyl*Fluoride Hydrochloride
RAD 1610144
2389
40% Acrylamide/Bis 19:1
RAD 1610146
2390
40% Acrylamide/Bis 29:1
RAD 1610148
2391
40% Acrylamide/Bis 37.5:1
NDS EC850
2101
AccuGel™ 19:1
AMR 27-6200-01
2340
Acetyl CoA
BMB 101893
2102
Acetyl CoA
BMB 1685791
2104
Acrylamide
FSH BP170500
2224
Acrylamide
RAD 1610101
2347
Acrylamide
LTI 30391-023
4185
Agar - Bacterial Select
DIF DF0145170
5889
Agar - Granulated
FSH BP1423500
4308
Agar - Granulated
LTI 15510-019
2105
Agarose
LTI 15510-027
2106
Agarose
FMC 50100
2234
Agarose (LMP) SeaPlaque®
FMC 50101
2263
Agarose (LMP) SeaPlaque®
LTI 10975-035
1289
Agarose 1000
FMC 50090
2321
Agarose NuSieve® 3:1
FMC 50080
2233
Agarose NuSieve® GTG®
FMC 50004
2236
Agarose SeaKem® LE
Unit
CC Price
1L
$
1L
$
500ML
$
1L
$
0.5 ml
$
500 ml
$
500 ml
$
500 ml
$
100 mg
$
500 ml
$
500 ml
$
500 ml
$
450 ml
$
10 mg
$
10 mg
$
500 g
$
500 g
$
500 g
$
500 g
$
500 g
$
500 g
$
100 g
$
500 g
$
125 g
$
25 g
$
100G
$
125 g
$
125 g
$
500 g
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 58
12.70
11.65
30.10
9.51
53.89
39.32
39.32
39.32
66.71
45.57
45.57
45.57
43.76
53.56
52.56
95.70
74.59
99.19
64.27
59.09
68.93
73.39
256.80
289.24
105.93
181.90
223.98
278.01
285.54
FMC
FSH
RAD
CCS
SIG
BMB
FSH
USB
BMB
RAD
FSH
RAD
BMB
INV
RAD
FSH
SIG
RAD
DIF
FSH
BMB
BMB
FSH
CCS
FSH
BMB
BMB
RAD
USB
AMR
FSH
SIG
BMB
FSH
RAD
FSH
SIG
CLN
CLN
CLN
CLN
SIG
LTI
FSH
LTI
SIG
SIG
AMR
AMR
BMB
FSH
AMR
LTI
BMB
FSH
50000
BP179100
1610700
2111
A6279-10ml
1383221
BP1610100
US12387
874515
1610786
BP17125
1610201
1685830
R21001
1610710
BP168500
B2518-100mg
1610404
DF0230173
BP1424500
757306
757292
BP210500
2117
BP339500
1873580
1836153
1610400
70468
17-0340-01
BP1585100
D2650-5x5ml
100032
BP1725
1610611
BP2311
D2650-100ml
630415
630414
630413
630416
D9891-25g
15575-020
BP120500
15585-011
E1510
E4391
17-1440-03
17-1440-02
1814320
BP227500
17-0756-01
15514-011
100149
BP3811
2235
4337
2352
2111
5912
2379
2405
2113
2303
6058
4338
2350
2114
4635
5908
2406
2446
5911
5890
4309
2115
2116
2226
2117
4339
5522
5521
5909
2121
2212
4440
5914
2122
2239
6060
2407
6062
5992
5989
5981
5991
2445
2283
2408
2277
2375
2376
2126
2125
2127
2227
2213
2130
2131
2230
Agarose SeaKem® LE
125 g
Ammonium Persulfate
100 g
Ammonium Persulfate
10 g
Ampicillin - 25mg/ml
10 ml
Aprotinin from Bovine Lung
10 ml
BCIP
3ml/150mg
BCIP
100 mg
BCIP
100 mg
Bestatin
10 mg
Bio-Safe Coomasie Stain
1L
Bis-Acrylamide
25 g
Bis-Acrylamide
50 g
Bis-Acrylamide
25 g
Blasticidin
50 MG
BME (2-mercaptoethanol)
25ml
Boric Acid
500 g
BOVINE SERUM ALBUMIN MOLECULAR BIOLOGY GRADE 100g
Bromophenol Blue
10g
Casamino Acids
500 g
Casamino Acids
500 g
Cesium Chloride
1 kg
Cesium Chloride
100 g
Cesium Chloride
500 g
Chloramphenicol 34 mg/ml
10 ml
Citric Acid
500 g
Complete EDTA Free 20 Tabs
EA
Complete Mini 25 Tabs
EA
Coomassie™ Brilliant Blue R-250
10g
Denhardt's Solution
50 ml
Dextran Sulfate
100 g
Dextran Sulfate
100 g
Dimethyl Sulfoxide Hybri-Max® Sterile*Filtered
25 ml
Dithiothreitol (DTT)
5g
Dithiothreitol (DTT)
5g
Dithiothreitol (DTT)
5g
DMSO
1L
DMSO
100 ml
DO Supplement-His
1 ea
DO Supplement-Leu
1 ea
DO Supplement-Trp
1 ea
DO Supplement-Ura
1 ea
DOXYCYCLINE HYDROCHLORIDE
25g
EDTA 0.5M (4 x 100ml)
400 ml
EDTA Disodium Salt Dihydrate
500 g
Ethidium Bromide (ETBR) 10mg/ml
10 ml
Ethidium Bromide Aqueous Solution 10mg/ml
10 ml
Ethidium Bromide Tablets 10mg
10 ea
Ficoll-Paque® Plus solution
6x500ml
Ficoll-Paque® solution
6x100ml
Formamide
500 ml
Formamide
500 ml
Glutathione Sepharose
10 ml
Glycerol
500 ml
Glycine
1 kg
Glycine
1 kg
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 59
111.25
15.24
10.72
10.00
80.37
37.65
18.65
24.34
99.62
51.41
19.44
28.60
26.67
140.80
16.98
17.59
95.16
27.70
51.84
46.88
261.21
49.42
131.63
8.00
15.72
225.13
96.48
16.08
37.00
119.29
87.37
29.82
54.91
42.03
52.48
30.36
84.34
34.29
37.07
34.29
34.29
174.74
23.91
30.64
28.47
34.25
31.02
337.40
99.23
36.08
30.90
159.09
18.18
40.79
29.35
LTI
RAD
LTI
FSH
BMB
FSH
LTI
INV
INV
INV
BMB
FSH
FSH
CCS
CCS
CCS
LTI
SIG
DIF
FSH
CCS
MED
SIG
DIF
FSH
DIF
FSH
BIO
BMB
SIG
FSH
CLN
CLN
BMB
USB
INV
INV
INV
INV
INV
INV
INV
INV
RAD
BMB
BMB
DIF
FSH
AMR
SIG
SIG
FSH
LTI
BMB
BMB
15527-013
1610718
15502-016
BP221250
1685929
BP310100
15630-080
Q602-20
Q612-20
Q621-20
724815
BP16201
BP162010
6005
6006
6007
22700-025
L7533
DF0445174
BP1425500
6004
MT46-050-CM
L7658-1kg
DF0402170
BP1427500
DF0446173
BP1426500
3002-031
1017128
L2884-10mg
BP214500
630412
630411
1383213
19535
NP00061
NP0005
NP0007
NP0002
NP0001
NP0004
NP0006
LA0041
1610202
1429868
1359053
DF0118170
BP1420500
17-0891-01
H7033-125ml
H7033-1L
BP226500
15513-039
100995
1814303
2132
2354
2133
4442
2134
4441
4445
6092
6094
6095
2145
2238
5299
6005
6006
6007
4186
5916
5891
4310
6004
1294
5917
5893
4311
5892
4312
2420
2146
6063
2410
5905
5903
2378
2156
4011
6081
6082
6083
6084
6085
6086
6087
2395
2377
2172
5886
4313
2173
6066
5448
2231
2176
2237
2174
Glycine
Glycine
Guanidine HCL
Guanidine Isothiocyanate
Guanidine Thiocyanate
HEPES
HEPES Buffer Solution (1M)
imMedia™ Amp Blue
imMedia™ Kan Blue
imMedia™ Zeo Agar
IPTG
IPTG
IPTG
LB + Ampicillin (50ug/ml) Agar Plates
LB + Kanamycin (50ug/ml) Agar Plates
LB + Tetracycline (20ug/ml) Agar Plates
LB Agar
LB Agar - EZMix®
LB Agar - Miller
LB Agar - Miller
LB Agar Plates
LB BROTH
LB Broth - EZMix®
LB Broth - Lennox
LB Broth - Lennox
LB Broth - Miller
LB Broth - Miller
LB Medium Capsules
Leupeptin
Leupeptin Hemisulfate from Microbial Source
Magnesium Chloride
Minimal SD Agar Base
Minimal SD Base
NBT (Nitro Blue Tetra)
NBT (Nitro Blue Tetra)
NP Transfer Buffer (20X)
NuPAGE® Antioxidant
NuPAGE® LDS Sample Buffer (4X)
NuPAGE® MES SDS Running Buffer (20X)
NuPAGE® MOPS SDS Running Buffer (20X)
NuPAGE® Sample Reducing Agent (10X)
NuPAGE® Transfer Buffer (20X)
NuPAGE® Tris-Acetate SDS Running Buffer (20X)
PDA
Pefabloc® SC
Pepstatin (was #600160)
Peptone
Peptone
Percoll®
Perfect Hyb Plus Hybridization Buffer
Perfect Hyb Plus Hybridization Buffer
Phenol
Phenol Saturated (Buffer)
Phenol Saturated (Tris)
Phenol (was 100300)
500 g
1 kg
500 g
250 g
500 g
100 g
100 ml
ea
ea
ea
1g
1g
10 g
10 pl
10 pl
10 pl
500 g
6 x 500 ml
500 g
500 g
10 pl
1L
1 kg
500 g
500 g
500 g
500 g
1 kg
25 mg
10 mg
500 g
1 ea
1 ea
3 ml
1g
1L
15ml
10ml
500ml
500ml
250ul
125ml
500ml
10 g
100 mg
10 mg
500 g
500 g
1L
125 ml
1L
500 g
100 ml
100 ml
500 g
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 60
23.53
29.49
65.42
58.61
135.71
32.36
26.77
225.64
177.96
276.96
32.16
27.17
202.45
10.00
10.00
10.00
47.15
21.68
44.71
54.23
10.00
13.78
122.11
20.43
26.88
20.43
26.94
107.64
105.11
57.64
22.37
139.00
92.66
36.87
59.94
100.04
15.28
7.64
43.07
43.07
7.64
13.43
41.22
75.96
90.99
47.07
41.30
46.06
226.82
75.03
427.49
63.92
58.24
48.63
92.56
FSH
FSH
FSH
FSH
SIG
FSH
BMB
SIG
SIG
SIG
SIG
SIG
SIG
SIG
BMB
BMB
BMB
FSH
FSH
BMB
NDS
NDS
NDS
LTI
NDS
NDS
NDS
NDS
NDS
NDS
BMB
FSH
RAD
BMB
LTI
LTI
BMB
FSH
LTI
USB
RAD
BMB
RAD
SIG
CCS
SIG
SIG
BMB
BMB
RAD
LTI
LTI
FSH
FSH
LTI
BP1750I100
BP1750I400
BP1752I100
BP1752I400
P7626-5g
BP366500
1697498
P8465-5ml
P8465-25ml
P2714
P8340-1ml
P8340-5ml
P8849-5ml
P8215-1ml
3115879
1373200
1000144
BP1700100
BP1700500
3115828
EC890
EC-892
EC-893
15553-027
EC835
EC830
EC840
EC833
EC836
EC838
1667262
BP166500
1610301
1666681
15557-036
15591-035
100168
BP2201
15558-026
US75824
1610733
100139
1610800
T9179
2197
T9424-25ml
T9424-100ml
604205
604203
1610719
15567-027
15568-025
BP1521
BP13381
15506-017
5300
5301
5302
5303
5918
2411
2345
2403
2404
2398
2401
2402
6065
2399
5136
2444
5518
5211
5304
5526
2177
2341
2342
2284
2179
2180
2181
2182
2184
2183
2185
2232
5910
2186
2279
2285
2189
4443
2275
2297
2355
2196
2351
5922
2197
5923
6064
2198
2199
2353
2281
2282
4444
5305
2202
Phenol Saturated pH 6.6
Phenol Saturated pH 6.6
Phenol/Chloroform/Isoamyl Alcohol
Phenol/Chloroform/Isoamyl Alcohol
Phenylmethylsulfonyl Fluoride
Potassium Chloride
Protease Inhibitor Cocktail Tablets (Complete)
Protease Inhibitor Cocktail-Bacterial
Protease Inhibitor Cocktail-Bacterial
Protease Inhibitor Cocktail-General
Protease Inhibitor Cocktail-Mammalian w/o Metal Chelators
Protease Inhibitor Cocktail-Mammalian w/o metal chelators
Protease Inhibitor Cocktail-Poly His Tagged
Protease Inhibitor Cocktail-Yeast/Fungal
Proteinase K
Proteinase K
Proteinase K
Proteinase K
Proteinase K
Proteinase K Solution (20 mg/ml)
ProtoGel® (30% Acrylamide/0.8% Bis)
ProtoGel® Buffer
ProtoGel® Stacking Buffer
SDS 10% (4x100ml)
SequaGel® Buffer
SequaGel® Concentrate (20% Acryl.) 19:1
SequaGel® Diluent
SequaGel® Sequencing System
SequaGel®-6 6% Monomer Sequencing Soln (includes EC-841)
SequaGel®-8 8% Monomer Sequencing Soln (includes EC-841)
Sodium Dodecyl Sulfate
Sodium Dodecyl Sulfate (SDS)
Sodium Dodecyl Sulfate (SDS)
SSC 20X
SSC 20X
SSPE 20X
Sucrose
Sucrose
TAE 10X
Taurine
TBE 10X
TEMED
TEMED
Terrific Broth (Modified)-EZMix®
Tetracycline 12.5mg/ml
Tri Reagent
Tri Reagent
Tris
Tris
Tris
TRIS pH 7.5
TRIS pH 8.0
Tris Base
TRIS EDTA Solution 100X
Tris Hydrochloride
100 ml
400 ml
100 ml
400 ml
5g
500 g
20 tabs
5 ml
25 ml
1 btl
1 ml
5 ml
5 ml
1 ml
100 mg
25 ml
500 mg
100 mg
500 mg
5 ml
1L
450 ml
200 ml
400 ml
200 ml
1L
1L
1 kit
1L
1L
100 g
500 g
100g
4L
4L
4L
1 kg
1 kg
4L
1 kg
1L
50 g
5 ml
6 x 500ml
10 ml
25 ml
100 ml
1 kg
500 g
1 kg
1L
1L
1 kg
1L
500 g
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 61
33.30
71.96
30.24
72.45
43.08
15.18
225.13
29.43
118.05
24.85
29.74
118.82
118.05
29.49
59.61
313.77
61.97
61.80
285.55
90.99
72.98
19.36
16.00
25.51
10.00
54.20
20.90
85.58
37.44
39.98
29.81
61.80
25.91
80.80
49.53
79.75
32.16
20.57
58.24
115.06
21.02
32.16
13.40
67.48
8.00
24.11
75.55
57.26
36.08
55.40
17.73
19.28
51.75
55.41
50.10
RAD
FSH
FSH
BMB
SIG
SIG
SIG
SIG
SIG
LTI
MED
FSH
DIF
RAD
FSH
LTI
FSH
BMB
BMB
CCS
DIF
FSH
LTI
1610732
BP152500
BP13071
789704
T6066-1Kg
T1503-1kg
T3253-1kg
T2444
T2694
15596018
MT25-900-CI
BP1421500
DF0123173
1706531
BP169500
15505-050
BP5611
745740
651745
2207
DF0127179
BP1422500
22712-020
2397
2412
2413
2203
5921
5919
5920
1287
1288
5457
1293
4314
5887
6059
2240
2204
2409
2205
2206
2207
5888
4315
4189
TRIS/Glycine/SDS 10X Buffer
Tris-Base
Tris-Glycine (10X)
Triton® X-100
TRIZMA® Base Molecular Biology Reagent Grade
TRIZMA® Base Reagent Grade
TRIZMA® Hydrochloride Reagent Grade
Trizma-Hcl, 1 M Stock Solution, pH 7.6
Trizma-Hcl, 1 M Stock Solution, pH 8.0
Trizol
Trypan Blue Solution
Tryptone
Tryptone Peptone
Tween 20
Urea
Urea
Water(Sterile Nuclease Free)
X-gal
X-gal
X-gal
Yeast Extract
Yeast Extract
YT Broth 2X
1L
500 g
1L
100 ml
1 kg
1 kg
1 kg
1L
1L
200 ml
100ML
500 g
500 g
100 ml
500 g
2 kg
1L
1g
250 mg
1g
500 g
500 g
500 g
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
PURIFICATION KITS AND COLUMNS
.................................................................................. CO
Mfgr. Mfgr No
QIA 34698
CLN 636055
CLN 636060
QIA 301425
QIA 12362
INV K1593-02
BIO 1102-200
BIO 1101-200
BIO 1101-400
BIO 1001-400
BIO 1001-600
SIG May-00
BMB 1754777
BMB 1754785
QIA 12643
BIO 2055-400
INV K1520-02
AMR 27-5325-01
AMR 27-5330-01
AMR 27-5120-01
AMR 27-5130-01
AMR 17-0855-02
QIA 30410
PRO Z5400
AMR 27-5335-01
QIA 51104
QIA 51304
Code
4603
4533
4536
4601
4502
5221
4614
4450
4569
4269
4448
4539
4512
4513
4602
4451
5220
4467
4507
4509
4299
4148
4503
4528
4297
4318
4319
Item Description
Anti-His Antibody Selector Kit (3 x 3 µg)
CHROMA SPIN-10 Columns
CHROMA SPIN-100 Columns
Effectene Transfection Reagent (1 ml)
EndoFree Plasmid Maxi Kit (10)
FastTrack® 2.0 Kit
GeneClean Turbo
GeneClean® DNA Purification Spin Kit
GeneClean® DNA Purification Spin Kit
GeneClean® II DNA Purification Kit
GeneClean® III DNA Purification Kit
Genelute Agarose Spin Column
High Pure Plasmid Isolation Kit
High Pure Plasmid Isolation Kit
Hispeed Midi Kit (25) 45 minutes/200ug
Lambda Quick! Phage DNA Isolation Spin Kit
Micro-FastTrack™ Kit
MicroSpin G-25 Columns
MicroSpin G-50 Columns
MicroSpin S-200 HR Columns
MicroSpin S-300 HR Columns
NICK® Columns
Ni-NTA Superflow
PolyATtract® System 1000 RNA Isolation System
ProbeQuant™ G-50 Micro Columns
QIAamp™ Blood Kit
QIAamp™ DNA Mini Kit
Unit
3x3ug
1 ea
1 ea
1 ml
kit
6 rxn
50 prp
50 prp
100 prp
300 prp
600 prp
70 ea
50 col
250 col
45min/200ug
25 prp
20 rxn
50 col
50 col
50 col
50 col
50 col
25 ml
1 kit
50 col
50 prp
50 prp
CC Price
$
70.88
$
112.10
$
112.12
$
197.38
$
211.73
$
322.10
$
57.36
$
78.53
$
139.44
$
123.53
$
180.01
$
76.00
$
47.85
$
206.30
$
220.71
$
100.60
$
369.52
$
119.71
$
119.71
$
119.71
$
119.71
$
219.73
$
221.60
$
279.25
$
119.71
$
90.61
$
100.48
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 62
19.66
32.76
31.44
25.10
72.53
71.70
113.46
16.69
16.69
149.82
6.63
37.83
36.39
35.81
21.21
67.36
17.59
147.47
86.29
65.00
51.68
54.35
33.58
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
QIA
RAD
RAD
RAD
BMB
BMB
BMB
BMB
BMB
QIA
QIA
BIO
QIA
PRO
PRO
PRO
PRO
PRO
PRO
PRO
PRO
PRO
PRO
PRO
PRO
30210
20021
12191
12162
12163
12165
12181
12143
12123
27106
27104
28144
28706
28704
28304
28104
79654
7326130
7326120
7326100
1273922
1273990
1273965
1273973
1274015
74106
74104
2070-400
301305
PRA7280
PRA7290
PRA7270
A7300
PRA7640
A7211
PRA7141
A7170
A7100
A7510
A1330
A1460
4316
4158
4564
4159
4317
4565
4281
4161
4163
4566
4203
4567
4568
4204
4504
4255
4282
5437
5438
4522
4165
4166
4167
4326
4168
4595
4256
4214
4547
4142
4143
4144
4145
4195
4292
4293
4147
4146
4291
4529
4531
QIAexpress™ Ni-NTA Agarose Kit
QIAEX™ II Gel Extraction Kit
QIAGEN Plasmid Giga Kit
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Mega Kit
QIAGEN Plasmid Midi Kit
QIAGEN Plasmid Mini Kit
QIAprep Spin Miniprep Kit
QIAprep™ Spin Miniprep Kit
QIAquick 8 PCR Purification Kit
QIAquick Gel Extraction Kit
QIAquick™ Gel Extraction Kit
QIAquick™ Nucleotide Removal Kit (50)
QIAquick™ PCR Purification Kit
QIAshredder™
Quantum Maxi-Prep Kit
Quantum Midi-Prep Ket
Quantum™ Prep Plasmid Miniprep
Quick Spin™ G25 DNA
Quick Spin™ G25 RNA
Quick Spin™ G50 DNA
Quick Spin™ G50 DNA
Quick Spin™ G50 RNA
RNeasy Mini Kit
RNeasy™ Mini RNA Kit
RPM™ Rapid Pure Plasmid Miniprep Kit
SuperFect Transfection Reagent (1.2 ml)
Wizard™ DNA Clean-up System
Wizard™ Lambda System
Wizard™ Maxipreps System
Wizard™ Megapreps System
Wizard™ Midipreps System
Wizard™ Minicolumns
Wizard™ Miniprep DNA Purification Resin
Wizard™ PCR Preps System
Wizard™ Plus Miniprep System
Wizard™ Plus Miniprep System
Wizard™ Plus SV
Wizard™ Plus SV
25 ml
150 prp
5 prp
10 col
25 col
100 prp
5 col
25 col
25 col
250 prp
50 rxn
400 prp
250 prp
50 prp
kit
50 rxn
50 cap
10 prp
20 prp
kit
20 col
20 col
20 col
50 col
20 col
250 prp
50 prp
120 prp
3 mg/ml
100 col
20 col
10 col
5 col
25 col
250 col
250 ml
50 col
50 col
250 col
50 rxn
250 rxn
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
176.74
101.38
324.78
151.62
359.77
1,327.82
187.51
177.64
122.02
253.00
58.32
538.31
329.26
70.88
70.88
70.88
48.45
107.23
107.23
107.23
71.38
103.54
71.38
133.35
103.54
768.88
174.95
139.43
171.36
83.87
56.77
90.35
86.36
111.78
99.95
94.35
59.20
65.09
222.20
42.56
189.73
FILM AND MEMBRANES
....................................................................................................... FM
Mfgr.
KOD
KOD
KOD
FSH
NEN
KOD
KOD
FUJ
FUJ
Mfgr No
V8701302
V8715187
V8294985
57144
NEF-978Y
EB135-24
EB135-36
04-441-118
04-441-115
Code
2306
2307
2364
2118
2260
2382
2383
2325
2324
Item Description
Biomax™ Film MR-1 8x10in
Biomax™ Film MR-1 35x43cm
Biomax™ Film MS-1 8x10in
Chromatography Paper 3MM 46X57cm
Colony/Plaque Screen™ w/orient. holes 137mm
Elite II Slide Film 100 - 24 Exposures
Elite II Slide Film 100 - 36 Exposures
Fuji RX Film NIF 14x17in
Fuji RX Film NIF 8x10in
Unit
50 sht
50 sht
50 sht
100/pk
50/bx
1 roll
1 roll
100 sht
100 sht
CC Price
$
$
$
$
$
$
$
$
$
138.93
300.20
118.70
85.24
231.21
6.76
9.13
305.28
111.69
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 63
NEN
NEN
NEN
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
AMR
MIL
MIL
MIL
RAD
MSI
MSI
MSI
MSI
MSI
MSI
MIL
MIL
RAD
RAD
RAD
INV
INV
MSI
MSI
POL
POL
NEN
INV
RAD
RAD
MIL
MIL
MIL
MIL
MIL
MIL
MIL
NEF-986
NEF-976
NEF-984
RPN2020D
RPN303D
RPN137N
RPN203N
RPN2222N
RPN303N
RPN82N
RPN87N
RPN303E
RPN137B
RPN203B
RPN2020B
RPN303B
RPN87B
RPN2020F
RPN1645
RPN1677L
RPN2114K
RPN3114K
RPN1678K
INYC13750
IPVH20200
IPVH00010
1620174
N00HN13750
NJ0HY13750
EP4HY408F0
EP4HY13750
EP4HYA0010
EP4HY00010
HATF08250
HATF13750
1620147
1620146
1620115
LC2000
LC2001
WP4HY08250
WP4HY00010
444159
444191
NEF-1002
LC2002
1620181
1620186
SCGPU02RE
SCGPU05RE
SCGPU11RE
SCGPU01RE
SCGP00525
SCGPS10RE
SCGPT10RE
2250
2246
2249
2290
2333
2359
2139
2136
2137
2138
2358
2289
2357
2135
2292
2293
2356
2295
2308
4015
2286
2287
2143
2439
2214
2215
2440
2432
2150
2428
2157
2158
2159
2217
2216
2372
2370
2373
2441
2442
2164
2163
2322
2323
2253
2443
2371
2368
2271
2272
2273
2433
2339
2434
2435
Genescreen Plus® Hybridization Mem. 20x20cm
10/bx
Genescreen Plus® Hybridization Mem. 30x45cm
5 sht
Genescreen™ Hybridization Mem. 20x20cm
10/bx
Hybond™ ECL™ 20x20cm
10 sht
Hybond™ ECL™ 30cmx3m
1 roll
Hybond™ Nylon 137mm
50/bx
Hybond™ Nylon 20cmx3m
1 roll
Hybond™ Nylon 22x22cm
10/bx
Hybond™ Nylon 30cmx3m
1 roll
Hybond™ Nylon 82mm
50/bx
Hybond™ Nylon 87mm
50/bx
Hybond™-C extra sup nitrocellulose 30cmx3m
1 roll
Hybond™-N+ charged nylon 137mm
50/bx
Hybond™-N+ charged nylon 20cmx3m
1 roll
Hybond™-N+ charged nylon 20x20cm
10 sht
Hybond™-N+ charged nylon 30cmx3m
1 roll
Hybond™-N+ charged nylon 87mm
50/bx
Hybond™-PVDF 20x20cm
10 sht
Hypercassette™ (beige) 35x43cm
1 ea
Hyperfilm MP Enveloped, 8x10in
50 sheets
Hyperfilm™ ECL™ 8x10in
25 sht
Hyperfilm™ ECL™ 8x10in
75 sht
Hyperfilm™ MP 8x10in
75 sht
Immobilon-NY+ 137mm DISC(Colony Plaque)
50/pk
Immobilon™ P 20x20cm (Western Blot)
10/pk
Immobilon™ P 26.5cmx3.75m (Western Blot)
1 roll
Immun-Blot PVDF
10 sht
Magna 137mm Notched
50/pk
Magnagraph 137mm
50/pk
Nitrobind 7x8.4
10/pk
Nitrobind Pure 137mm
50/pk
Nitrobind Pure 20cmx3m
1 roll
Nitrobind Pure 30cmx3m
1 roll
Nitrocellulose 82mm Colony & Plaque
50/pk
Nitrocellulose 137mm Colony & Plaque
50/pk
Nitrocellulose Membrane (.20u) 13.5x16.5cm
10 sht
Nitrocellulose Membrane (.20u) 7x8.4cm
10 sht
Nitrocellulose Membrane (.45u) 33cmx3m
1 roll
Nitrocellulose Membrane (0.2 µm) Filter Paper Sandwich 20/pk
Nitrocellulose Membrane (0.45 µm) Filter Paper Sandwich 20/pk
Nitropure Supp 82mm
50/pk
Nitropure Supp 30cmx3m
1 roll
Polaroid Film Type 57
pk
Polaroid Film Type 667
pk
Polyscreen® PVDF 30cmx3m
1 roll
PVDF Membrane/Filter Paper Sandwich
20/pk
Sequi-Blot™ PVDF Membrane (.20u) 15x15cm
10 sht
Sequi-Blot™ PVDF Membrane (.20u) 7x8.4cm
10 sht
Stericup Filtr. & Stor.-PES 250ml .22µ
12/pk
Stericup Filtr. & Stor.-PES 500ml .22µ
12/pk
Stericup Filtr. & Stor.-PES 1000ml .22µ
12/pk
Stericup Filtr. & Stor.-PES 150ml .22µ
12/pk
Steriflip-GP Filter Unit 50µl
25/pk
Steritop-GP Filtr 1000 ML 33 mm neck 0.22µm
12/pk
Steritop-GP Filtr 1000 ML 45 mm neck 0.22µm
12/pk
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
150.24
218.43
149.17
136.93
168.86
182.30
195.74
157.94
214.22
89.05
99.13
236.06
231.86
200.78
195.74
223.46
126.85
178.51
204.16
144.28
79.55
206.34
206.34
156.48
101.81
125.90
55.49
107.32
125.41
23.91
104.99
99.17
146.88
49.63
101.81
126.00
46.47
171.57
104.21
104.21
50.75
140.31
47.95
24.07
181.14
101.89
99.19
64.34
42.64
66.82
95.45
33.09
80.25
65.54
65.54
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 64
MIL
MIL
MIL
MIL
MIL
MIL
KOD
KOD
KOD
NEN
NEN
RAD
SCGPS05RE
SCGPT05RE
SLGV025LS
SLHVO25LS
SLGS0250S
SLHA0250S
V1651678
V1651579
V1651454
NEF-586
NEF-596
1620192
2336
2335
2266
2264
2267
2265
2331
2332
2211
2388
2385
2362
Steritop-GP Filtr 500 ML 33 mm neck 0.22µm
Steritop-GP Filtr 500 ML 45 mm neck 0.22µm
Syringe fltr-Durapore PVDF/PVC .22µ
Syringe fltr-Durapore PVDF/PVC .45µ
Syringe fltr-mixed esters of cellulose .22µ
Syringe fltr-mixed esters of cellulose .45µ
X-Omat™ AR-2 interleaved film 35x43cm
X-Omat™ AR-2 interleaved film 8x10in
X-Omat™ AR-5 non-interleaved film 8x10in
X-Omat™ Blue 5x7
X-Omat™ Blue 8x10in
Zeta-Probe® GT Membrane15x15cm
12/pk
12/pk
50/pk
50/pk
50/pk
50/pk
50 sht
50 sht
50 sht
100/bx
100/bx
15 sht
$
$
$
$
$
$
$
$
$
$
$
$
41.36
41.36
70.63
66.49
64.27
62.36
353.46
182.70
144.05
57.53
135.27
180.51
IMMUNOLOGICAL REAGENTS
.............................................................................................. IM
Mfgr.
AMR
BMB
AMR
AMR
INV
CCS
LTI
LTI
LTI
COL
PRO
Mfgr No
27-4577-01
1583816
NA931
NA934
R96025
NA
15918-014
15920-010
19543-024
CB40066
S3841
Code
3186
3195
3143
3144
4636
3207
3111
3112
3116
3208
3203
Item Description
Anti-GST Antibody
Anti-HA (12CA5)
Anti-Mouse Ig - HRP conjugated
Anti-Rabbit Ig - HRP conjugated
Anti-V5 Antibody
myc 1 - 9E10 antibody
Protein A Agarose
Protein G Agarose
Streptavidin Red 670™
TNF-a
Western Blue Stabilized Substrate for AP
Unit
0.5ml
200 µg
1 ml
1 ml
50 ul
0.5 mg
5 ml
5 ml
100 µg
10µg
1 ea
CC Price
$
207.13
$
202.38
$
214.02
$
165.42
$
148.21
$
20.00
$
166.69
$
253.01
$
157.50
$
225.88
$
30.78
MOLECULAR BIOLOGY KITS
................................................................................................KT
Mfgr. Mfgr No
Code Item Description
Unit
CC Price
BMB 1483188
5519
1st Strand cDNA Kit
30 rxn
$
ENZ 42655-10
4630
BioArray High Yield RNA Transcript Labeling Kit
10 rxn
$
ENZ 42655-20
4631
BioArray High Yield RNA Transcript Labeling Kit
20 rxn
$
CLN 631312
6008
Cal Phos Mammalian Transfection Kit
sm box
$
RAD 5000112
5941
DC Protein Assay Kit II
1 kit
$
QIA 69504
5473
DNeasy Tissue Kit
50 $
SIG XNAT-100RXN 4016
Extract n Amp Red
100 rxn
$
AMR 27-9261-01
5314
First Strand cDNA Synthesis Kit
1 kit
$
AMR 27-4590-01
5363
GST Detection Module
50 rxn
$
AMR 27-4570-01
5362
GST Purifications Bulk Module
5 prps
$
BMB 1585584
5281
High Prime DNA Labeling Kit
50 rxn
$
BMB 1585592
5282
High Prime DNA Labeling mix for dCTP
50 rxn
$
MLG 11452-018
5622
High Pure Maxi Kit
10rxn
$
BMB 1796828
5520
High Pure PCR Template Prep
EA
$
QIA 12662
5471
Hispeed Plasmid Maxi Kit
10 prep
$
QIA 12663
5472
Hispeed Plasmid Maxi Kit
25 prep
$
LTI 18160-010
5133
Nick translation Kit
50 rxn
$
INV K4900-01
6098
pcDNA3.1© Directional TOPO® Expression Kit (Top 10 Cells) 20 rxn
$
STR 211190
4260
pCR-Script™ Amp Cloning Kit, (3 parts, -80,-20, RT)
1 kit
$
STR 300385
5138
Prime-It® II Random Priming Kit
30 rxn
$
236.11
705.00
1,380.75
143.63
108.13
98.69
147.21
207.13
182.72
266.20
207.09
188.26
122.65
131.78
183.02
442.31
128.09
360.57
263.71
155.58
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 65
RAD
RAD
AMR
STR
LTI
BMB
MLG
MLG
MLG
AMR
USB
USB
RAD
LTI
INV
PRO
PRO
PRO
PRO
PRO
PRO
INV
INV
INV
INV
INV
INV
5000006
5000002
27-9558-01
200518
18428-011
1004760
11456-019
11458-015
11453-016
27-9240-01
70170
70140
1610449
11904-018
K2050-01
L4600
L5020
L4950
L4610
L1170
L5010
K4500-01
K4550-01
K4520-01
K4650-01
K4575-01
K2800-20
5937
5939
2167
5934
5227
5139
5625
5624
5623
4300
5278
5275
5360
5226
5389
5930
5931
5929
5928
5933
5932
6097
5397
5466
5398
5446
6096
Protein Assay Kit Dye Reagent Concentrate
Protein Assay Kit II
PureTaq RTG PCR Beads
QuickChange™ Site-Directed Mutagenesis Kit
Rad Prime Labeling Kit
Random Primed DNA Labeling Kit
Rapid Gel Extraction Kit
Rapid PCR Purification Kit
Rapid Plasmid Mini Prep
Ready-To-Go DNA Labelling Beads (-dCTP)
Sequenase™ PCR Product Seq. Kit
Sequenase™ Quick Denature Seq. Kit
Silver Stain Plus Kit
Superscript First-Strand Synthesis System for RT-PCR
TA Cloning Kit® with Dual Promoters (INVaF')
TNT®-SP6 Coupled Transcription/Translation
TNT®-SP6/T7 Coupled Transcription/Translation
TNT®-T3 Coupled Transcription/Translation
TNT®-T7 Coupled Transcription/Translation
TNT®-T7 QUICK Coupled Transcription/Translation
TNT®-T7/T3 Coupled Transcription/Translation
TOPO TA Cloning® Kit (TOP10 Cells)
TOPO TA Cloning® Kit (TOP10F' Cells)
TOPO TA Cloning® Kit (with LTI 4622 - DH5a Cells)
TOPO TA Cloning® Kit Dual Promoter (TOP10F' Cells)
TOPO TA Cloning® Kit for Sequencing (Top 10 Cells)
Zero Blunt® TOPO® PCR Cloning Kit (TOP 10 Cells)
1 kit
1 kit
0.5ML
1 kit
30 rxn
50 rxn
50rxn
50rxn
50rxn
1 kit
1 kit
1 kit
kit
50 rxn
20 rxn
1 kit
1 kit
1 kit
1 kit
1 kit
1 kit
20 rxn
20 rxns
20 rxns
1 ea
20 rxn
20 rxn
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
69.70
81.32
142.55
475.24
128.88
209.44
54.28
54.28
48.83
102.38
262.92
184.10
116.69
235.30
289.93
291.15
300.09
312.11
293.37
297.05
314.95
322.35
308.67
319.57
322.99
334.39
338.20
MOLECULAR BIOLOGY PRODUCTS
...................................................................................MB
Mfgr. Mfgr No
Code Item Description
Unit
BMB 1204530
4267
ABTS® Buffer
125 ml
BMB 1112422
4266
ABTS® Tablets (50mg)
20 tab
NEB E8021L
4330
Amylose Resin
100 ml
BMB 711454
4104
Bovine Serum Albumin (Nuclease free)
20 mg
LTI 15561-020
4618
Bovine Serum Albumin, Acetylated
150 mg
BMB 1213857
4233
Collagenase P
100 mg
BMB 1202375
4126
DOTAP (Liposomal Transfection Reagent)
2 ml
CLN 636831
6026
Express Hyb
250 ml
BMB 3045595
5440
ExtremeGENE (Transfection Reagent)
.4 ml
PRO Q4100
4248
fmol® DNA Sequencing System
1 kit
BMB 1815091
5421
FuGENE 6 (Nonliposomal Transfection Reagent)
.4 ml
BMB 1814443
5422
FuGENE 6 (Nonliposomal Transfection Reagent)
1 ml
BMB 901393
4131
Glycogen
20 mg
SIG G4877-10000iu 6067
GONADOTROPIN FROM PREGNANT MARES' SERUM 10000iu
LTI 15279-011
4135
Human Cot-1® DNA
500 µg
LTI 11668-019
6031
LipofectAMINE 2000
1.5ml
LTI 11668-027
6032
LipofectAMINE 2000
0.75ml
LTI 18324-012
4181
Lipofectamine™
1 ml
LTI 18324-020
4582
Lipofectamine™
4 x 1 ml
LTI 18324-111
4624
Lipofectamine™
.5 ml
LTI 18292-011
4138
Lipofectin® Reagent
1 ml
NEB S1404S
4331
m7GpppG 5'G RNA Cap Structure
25u
LTI 17502-048
4620
N2 Supplement
5 ml
CC Price
$
23.53
$
40.79
$
220.61
$
49.42
$
98.67
$
50.20
$
242.39
$
148.63
$
84.72
$
143.73
$
125.51
$
250.23
$
77.66
$
339.13
$
108.53
$
273.82
$
166.04
$
165.28
$
543.86
$
98.67
$
125.82
$
110.31
$
38.68
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 66
LTI
PRO
LTI
NEB
PRO
PRO
PRO
LTI
BMB
CCS
CCS
CCS
CCS
CCS
CCS
CCS
CCS
CCS
CCS
BMB
STR
PRO
AMR
BMB
AMR
AMR
FSH
PRO
PRO
PRO
PRO
STR
CLN
CLN
PRO
LTI
LTI
CLN
CLN
INV
18418-012
C1101
12252-011
N3032S
A1360
A1380
A3600
11514-015
108812
4463
4464
4465
4466
4190
4191
4194
4192
4193
4324
3335399
201220
L4960
27-2166-01
1034731
RPN1635
27-0816-01
2123621
N2515
N2511
N2115
N2111
201190
635501
635506
E2381
15596-026
15401-011
630440
630439
R250-01
6125
4268
4009
4254
5942
5943
4294
6034
4152
4463
4464
4465
4466
4190
4191
4194
4192
4193
4524
5525
4551
4169
4183
4170
4239
5357
4201
4176
4177
4174
4175
4552
6027
6028
5944
4182
4180
6030
6029
5417
Oligo (dT) 12-18 Primer
Oligo(dT) 15 primer
Oligofectamine Reagent
pBR322/Msp I Digest
pGEM®-T Easy I
pGEM®-T Easy II
pGEM®-T Vector I
Plus Reagent
Poly d(I-C)
Primer gt10 forward
Primer gt10 reverse
Primer gt11 forward
Primer gt11 reverse
Primer M13/pUC Forward 23mer
Primer M13/pUC Reverse 23mer
Primer Sp6 20mer
Primer T3 20mer
Primer T7 20mer
Primer T7 Term
Protector RNase Inhibitor
QuickHyb® Rapid Hybridization Buffer
Rabbit Reticulocyte Lysate
Random Primers (N)6
Random Primers (N)6
RapidHyb Hybridization™ Buffer
RNAguard Ribonuclease Inhibitor
RNase AWAY
RNasin® Recombinant Ribonuclease Inhibitor
RNasin® Recombinant Ribonuclease Inhibitor
RNasin® Ribonuclease Inhibitor
RNasin® Ribonuclease Inhibitor
Salmon Sperm DNA
Talon Metal Affinity Resin
Talon Superflow Metal Affinity Resin
Tfx-10 Transfection Reagent
TRIzol Reagent
Yeast tRNA
Yeastmaker Carrier DNA
Yeastmaker Yeast Transformation System
Zeocin
25 UG
20 µg
1 ML
50 µg
1 kit
1 kit
20u
.85 ml
10 Å260u
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
EA
250 ml
1 ml
50 Å260u
50 Å260u
125 ml
5000u
8.4 oz
10000u
2500u
10000u
2500u
10 x 1ml
10 ml
25 ml
1 ea
100 ml
25 mg
ea
kit
1g
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
45.40
33.28
188.12
40.44
89.93
151.91
74.41
87.73
66.68
20.00
20.00
20.00
20.00
20.00
20.00
20.00
20.00
20.00
20.00
64.32
154.95
94.41
117.35
111.39
65.37
55.13
17.05
173.32
61.49
163.43
64.86
156.16
69.64
194.59
143.21
78.80
44.91
199.23
203.86
175.07
PROTEIN AND MOLECULAR WEIGHT MARKERS
.................................……………………………............................MR
Mfgr.
LTI
LTI
NEB
LTI
PRO
LTI
PRO
LTI
NEB
PRO
Mfgr No
15615-016
15615-024
N3232S
10381-010
PRG6941
10787-018
G4471
15628-019
N3231S
G2101
Code
4123
4221
4584
4302
4588
4625
4585
4101
4583
4455
Item Description
1 kb DNA Ladder
1 kb DNA Ladder
1 kb DNA Ladder
1 kb DNA Ladder - RTS - ready load
1 kb DNA Step Ladder
1 kb Plus DNA Ladder
10 bp DNA Step Ladder
100 bp DNA Ladder
100 bp DNA Ladder
100 bp DNA Ladder
Unit
250 µg
1000 µg
100ug
100 app
300 lns
250 µg
50 µl
50 µg
50ug
250 µl
CC Price
$
93.19
$
309.87
$
40.44
$
53.13
$
58.57
$
84.88
$
57.08
$
41.70
$
40.44
$
56.38
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 67
PRO
LTI
LTI
LTI
PRO
LTI
AMR
LTI
PRO
LTI
BMB
BMB
BMB
BMB
BMB
BMB
BMB
RAD
NEB
LTI
NEB
PRO
LTI
INV
INV
INV
INV
LTI
NEB
NEB
PRO
LTI
RAD
NEB
RAD
RAD
RAD
AMR
AMR
LTI
LTI
INV
INV
INV
G6951
10380-012
10068-013
15613-011
G6961
10597-011
27-4005-01
10416-014
G4521
10748-010
1418009
1449460
821705
1062590
1336045
1498037
1721933
1610324
N3011S
15612-013
N3012S
G1711
10382-018
LC5600
LC5602
LC5677
LC5725
15611-015
N3026L
N3026S
G1761
10383-016
161-0374
P7708S
1610318
1610309
1610305
RPN756
RPN755
12308-011
15620-016
LC5925
LC5625
LC6060
4586
4304
4196
4102
4587
4498
4484
4499
4590
4619
4480
4231
4229
4230
4545
4232
4454
4515
4252
4136
4137
4486
4307
5182
4014
4596
4597
4151
4250
4251
4198
4305
4633
4546
4483
4482
4481
4234
4235
4632
4172
4599
4598
4600
100 bp DNA Step Ladder
100 bp Ladder - RTS - ready load
100 bp-2kb DNA Mass Ladder
123 Base Pair Ladder
200 bp DNA Step Ladder
25 bp DNA Ladder
50 bp DNA Ladder
50 bp DNA Ladder
50 bp DNA Ladder
BENCHMARK™ Prestained Protein Ladder
DNA Marker IV (74bp - 19329bp)
DNA Marker IX (72-1353bp)
DNA Marker V (8-587bp)
DNA Marker VI (154-2176bp)
DNA Marker VIII (19bp - 1114bp)
DNA Marker X (75-12216bp)
DNA Marker XIV (100 - 1500 bp) 100 bp ladder
Kaleidoscope Prestained Standards
Lambda DNA
Lambda DNA/HindIII Fragment
Lambda DNA/HindIII Fragment
Lambda DNA/HindIII Fragment
Lambda DNA/HindIII Fragment - RTS - ready load
MagicMark Western Protein Standard
MagicMark XP
Mark12™ Wide Range Molecular Weight Standard
MultiMark® Multi-Colored Standard
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment - RTS - ready load
Precision Plus Protein Dual Color Standard
Prestained Protein Molecular Weight Marker
Prestained Standards Broad
Prestained Standards High
Prestained Standards Low
Rainbow Marker™ (14300-250000)
Rainbow Marker™ (2350-46000)
Ready Load 1KB Plus DNA Ladder
RNA Ladder 0.24-9.5 kb
SeeBlue® Plus2 Pre-Stained Standard
SeeBlue® Pre-Stained Standard
SimplyBlue™ SafeStain
100 µl
100 app
200 µl
100 µg
100 µl
50 µg
200 µg
50 µg
100 lns
2 x 250u
50 µg
50 µg
50 µg
50 µg
50 µg
100 µg
50 µg
500 µl
250 µg
500 µg
150 µg
100µg
100 app
250ul
250ul
1.0 ml
500 µl
40 µg
250u
50u
50 µg
100 app
50 app
175 lns
500 µl
500 µl
500 µl
250 µl
250 µl
100 rxn
75 µg
500 µl
500 µl
1L
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
75.92
64.05
64.41
67.10
78.51
71.58
154.36
71.58
65.48
78.71
83.15
105.11
83.93
87.86
100.41
87.86
74.52
89.36
40.44
90.41
40.44
24.45
44.63
177.71
178.10
92.63
106.52
54.30
161.78
40.44
65.24
83.60
93.76
62.51
80.42
75.96
68.81
101.12
101.12
73.79
80.29
99.11
99.11
79.66
MISCELLANEOUS PRODUCTS
............................................................................................. MS
Mfgr.
FSH
AMR
FSH
FSH
FSH
FSH
Mfgr No
121311
NAMP100
212363
212362A
212368
212361
Code
6023
4296
4222
4228
4226
4227
Item Description
Aluminum Foil (18” x 50ft)
Amplify™
ART® 10 aerosol resistant pipet tip (racked)
ART® 1000E aerosol resistant pipet tip (racked)
ART® 100E aerosol resistant pipet tip (racked)
ART® 200 aerosol resistant pipet tip (racked)
Unit
ea
1L
960/pk
800/pk
960/pk
960/pk
CC Price
$
8.53
$
143.86
$
57.00
$
57.00
$
57.00
$
57.00
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 68
FSH
FSH
DEN
INV
INV
INV
RAD
RAD
RAD
TKR
TKR
QIA
QIA
DEN
DEN
DEN
DEN
DEN
RAI
RAI
RAI
RAI
RAI
RAI
RAI
RAI
RAI
RAI
RAI
RAI
DEN
DEN
DEN
DEN
DEN
DEN
FSH
212365
212364
R6651
P410-50
P450-50
P460-50
1652089
1652086
1652088
TKR-1301
C1201-CL
2003482
2003481
P-1125
P-1123
P-1096-FR
P-1121
P-1122
L-10
L-2
L-100
L-200
L-20
L-1000
P-2
P-10
P-1000
P-100
P-20
P-200
P3950-10
P3950-100
P3950-1000
P3950-20
P3950-200
P3950-5000
15610
4224
4223
4462
4519
4520
4521
5950
4517
4518
4621
4497
3345
3344
3327
3329
3325
3326
3328
6037
6036
6039
6040
6038
6035
5955
5956
5954
5952
5951
5953
4456
4458
4460
4457
4459
4461
6024
ART® 20E aerosol resistant pipet tip (racked)
ART® 20P aerosol resistant pipet tip (racked)
Chillete personal tube cooler -12 tubes at -20C
Electroporation Cuvettes
Electroporation Cuvettes
Electroporation Cuvettes
Gene Pulser® Cuvettes 0.1cm
Gene Pulser® Cuvettes 0.2cm
Gene Pulser® Cuvettes 0.4cm
Mini-Centrifuge
Mini-Centrifuge 6 x 1.5 ml
Oligo CD - $1,000
Oligo CD - $500
P-100 Filter Pipet Tips
P-1000 Filter Pipet Tips
P2/P10 Eatra Long Filter Pipet Tip
P-20 Filter Pipet Tips
P-200 Filter Pipet Tips
Pipete-Lite Magnetic Assist Pipette .5-10µl
Pipete-Lite Magnetic Assist Pipette 0.1- 2µl
Pipete-Lite Magnetic Assist Pipette 10-100µl
Pipete-Lite Magnetic Assist Pipette 20-200µl
Pipete-Lite Magnetic Assist Pipette 2-20µl
Pipet-Lite Magnetic Assist Pipette, 100-1000ul
Pipetman 0.1-2.0µl
Pipetman 0.5-10µl
Pipetman 100-1000µl
Pipetman 10-100µl
Pipetman 2-20µl
Pipetman 50-200µl
Pipet-mate Digital Pipette w/ tip ejector 10µl
Pipet-mate Digital Pipette w/ tip ejector 100µl
Pipet-mate Digital Pipette w/ tip ejector 1000µl
Pipet-mate Digital Pipette w/ tip ejector 20µl
Pipet-mate Digital Pipette w/ tip ejector 200µl
Pipet-mate Digital Pipette w/ tip ejector 5000µl
Wrapping Film (18in x 1000ft)
1000/pk
960/pk
1 ea
0.1cm
0.2cm
0.4cm
50 pk
50 pk
50 pk
ea
1 ea
EA
EA
EA
EA
EA
EA
EA
1 ea
1 ea
1 ea
1 ea
1 ea
ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
ea
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
56.98
57.00
28.60
115.26
115.26
115.26
116.69
124.21
116.69
250.00
185.59
1,000.00
500.00
47.30
47.30
47.30
47.30
47.30
227.60
227.60
199.60
199.60
199.60
199.60
266.92
266.92
234.45
234.45
234.45
234.45
158.95
152.01
152.01
152.01
152.01
159.29
18.66
NON-RADIOACTIVE KITS AND REAGENTS
.....................................................................………………………... NR
Mfgr. Mfgr No Code Item Description
Unit
CC Price
BMB 1096176
3154
Blocking Reagent
50 g
NEN NEL-601
5979
CDP-Star Nucleic Acid Chemiluminescence Reagent (Ready to Use) 1 kit
BMB 1644807
3181
Cell Proliferation Kit (WST-1)
ea
BMB 1500708
3123
Chemiluminescent Western Blot Kit
1000cm2
BMB 1585614
3178
DIG/Genius High Prime DNA Labeling and Detection Starter Kit II 1 kit
BMB 1277073
3175
Digoxigenin RNA Labeling Mix
20 rxn
BMB 1209256
3151
Digoxigenin-11-UTP
250 nmol
AMR RPN2132
5415
ECL™ Plus Western Blotting Detection Reagents
1 ea
AMR RPN2108
3117
ECL™ Western Blot Analysis Starter System
1 kit
AMR RPN2109
3118
ECL™ Western Blotting Detection Reagents
1000cm2
AMR RPN2106
3119
ECL™ Western Blotting Detection Reagents
4000cm2
AMR RPN2209
3142
ECL™ Western Blotting Detection Reagents
2000cm2
BMB 1175025
3130
GENIUS™ 4 RNA Labeling Kit
2x10rxn
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 69
$
$
$
$
$
$
$
$
$
$
$
$
$
79.23
134.25
278.47
76.87
192.18
92.56
123.94
181.69
131.75
103.33
225.60
162.74
311.42
BMB
PRO
PRO
PRO
BMB
BMB
NEN
NEN
NEN
NEN
BMB
BMB
BMB
1684795
E4030
E1501
E4530
2015200
2015196
NEL-201
NEL-103
NEL-104
NEL-105
1093274
1767305
1767291
3173
3185
3212
3211
6041
6042
5201
3120
3121
3122
3132
3180
3179
In Situ Cell Death Detection Kit - Fluorescein
Luciferase Assay System
Luciferase Assay System (10 pk) quote#5075
Luciferase Assay System–10 pack
Lumi Light
Lumi Light +
Renaissance™ Nucleic Acid Chemilum Rgt Kit
Renaissance™ Western Blot Chemilum Rgt
Renaissance™ Western Blot Chemilum Rgt
Renaissance™ Western Blot Chemilum Rgt
Sheep Anti-digoxigenin-AP
TUNEL Enzyme (optimized terminal transferase)
TUNEL Label (FITC dUTP + dNTPs)
50 rxn
100 assays
1000 assays
1000 assays
1 kit
1 kit
1 kit
1000cm2
2500cm2
5000cm2
150u
1 ea
1 ea
$
$
$
$
$
$
$
$
$
$
$
$
$
OTHER ENZYMES
.................................................................................................................. OE
Mfgr.
BMB
NEB
BMB
USB
BMB
PRO
PRO
BMB
NEB
PRO
PRO
NEB
LTI
AMR
LTI
LTI
BMB
BMB
PRO
LTI
LTI
AMR
PRO
PRO
BMB
LTI
NEB
PRO
AMR
LTI
PRO
AMR
AMR
LTI
PRO
BMB
BMB
Mfgr No
1417215
M0392S
1758250
70092Y
109118
M5108
M5101
713023
M0290S
M1821
M2051
M0209S
18010-025
27-0514-01
18047-019
18068-015
104159
776785
M6101
18052-019
10480-010
E70073Y
M1811
M2181
1008404
18012-021
M0210S
M2201
27-0925-01
28025-013
M1701
E2420Y
RPN1633
18021-014
M4281
1119915
109169
Code
5101
5307
5374
5102
5103
5105
5106
5108
5110
5256
5204
5112
5183
5373
5113
5225
5320
5114
5144
5454
5475
5287
5262
5366
5125
5120
5122
5123
5128
5127
5367
5131
5375
5141
5205
5143
5386
Item Description
Agarase
Agarase
Alkaline Phosphatase (shrimp)
Alkaline Phosphatase (shrimp)
AMV Reverse Transcriptase
AMV Reverse Transcriptase
AMV Reverse Transcriptase
Calf Intestinal Alkaline Phosphatase
Calf Intestinal Alkaline Phosphatase
Calf Intestinal Alkaline Phosphatase
DNA Polymerase I
DNA Polymerase I from E.coli
DNA Polymerase I (E Coli)
DNase 1
DNase I
DNase I - Amplification Grade
DNase I - Grade II
DNase I - RNase Free
DNase RQ1 RNase-free
E. Coli DNA Ligase
Elongase Enzyme Mix
Exonuclease I
Exonuclease III
Klenow - Exo Minus
Klenow - Labeling grade
Klenow - Sequencing Grade
Klenow - Sequencing Grade
Klenow Fragment
Maloney-MLV Reverse Transcriptase
Maloney-MLV Reverse Transcriptase
Maloney-MLV Reverse Transcripts
Mung Bean Nuclease
Rediprime™ kit for dCTP
Ribonuclease H
Ribonuclease H
RNase - DNase free
Rnase A
Unit
100u
100u
1000u
500u
1000u
1000u
300u
1000u
1000u
1000u
500u
500u
1000U
1000u
20000u
100u
100 mg
10000u
1000u
100u
100 rxn
2500u
5000u
100u
100u
100u
200u
150u
200u
40000u
10000u
3000u
30 rxn
30u
50u
500 µg
100 mg
CC Price
$
175.71
$
44.12
$
69.03
$
85.48
$
200.81
$
131.21
$
50.41
$
47.07
$
40.44
$
35.58
$
66.72
$
40.44
$
113.64
$
63.79
$
184.22
$
56.95
$
36.08
$
109.04
$
22.19
$
23.20
$
100.62
$
81.94
$
45.70
$
27.43
$
47.85
$
39.22
$
36.77
$
54.87
$
55.13
$
141.01
$
31.88
$
118.92
$
186.66
$
73.88
$
97.86
$
57.26
$
57.26
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 70
266.70
46.84
308.22
412.41
142.77
247.09
131.06
77.79
140.65
198.18
127.86
92.56
69.03
AMR
BMB
PRO
USB
USB
USB
USB
USB
AMR
LTI
PRO
EPI
LTI
LTI
LTI
LTI
LTI
PRO
BMB
LTI
LTI
NEB
NEB
PRO
NEB
BMB
LTI
LTI
NEB
PRO
BMB
BMB
LTI
NEB
NEB
PRO
AMR
PRO
AMR
LTI
PRO
AMR
BMB
E70054Y
109193
M5761
70710
70754
70721
70724
70775z
27-0808-01
18018-010
P1085
SL910K
11917010
18080044
12574-026
18064-014
18053-017
P2083
481220
15224-017
15224-025
M0202S
M0202L
M1801
M0202T
1004786
18005-017
18005-025
M0203S
M4211
1635379
709557
18004-010
M0201S
M0201L
M4101
27-0736-01
P2075
E2230Z
10533-065
M1871
27-0846-01
109541
5289
5388
5207
5147
5148
5150
5151
5239
5371
5156
5158
5322
5460
5478
5476
5159
5160
5161
5163
5164
5229
5165
5217
5208
5166
5167
5168
5464
5169
5209
5259
5171
5172
5173
5218
5175
5174
5179
5233
5194
5210
5368
5387
RNase H
RNase T1
S1 Nuclease
Sequenase™ dGTP labeling mix
Sequenase™ dGTP Nucleotide Kit
Sequenase™ Reagent Kit
Sequenase™ Stop Solution
Sequenase™ v 2.0
SP6 RNA Polymerase
Sp6 RNA Polymerase
Sp6 RNA Polymerase
SP6 RNA Polymerase (25u/µl)
Superscript Custom Choice Kit
Superscript III Reverse Transcriptase
SuperScript III, 1 step w/Platinum Taq 100
Superscript™ II Reverse Transcriptase
Superscript™ Reverse Transcriptase
T3 RNA Polymerase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase HC
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Rapid Ligation Kit (5 minutes)
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase FPLCpure
T7 RNA Polymerase
Terminal Deoxy Transferase (TdT)
Terminal Deoxy Transferase (TdT)
Terminal Deoxy Transferase (TdT)
Thrombin Protease
tRNA
250u
100000u
10000u
100 µl
1 kit
1 kit
1.2 ml
1000u
3000u
500u
1000u
10000u
10 rxn
10000u
100 rxn
10000u
10000u
1000u
100u
100u
500u
20000u
100000u
100u
20000u
100u
50u
250u
150u
100u
40 rxn
200u
200u
500u
2500u
100u
200u
1000u
2500u
500u
300u
500u
100 mg
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
51.19
33.73
19.27
25.20
119.41
90.93
17.46
417.91
152.79
48.07
94.73
102.02
656.60
158.13
351.55
149.91
154.90
22.97
40.79
26.09
115.12
44.12
176.48
22.98
44.12
73.74
31.89
103.79
40.44
48.19
104.33
160.81
50.03
36.77
147.07
27.43
73.24
30.39
345.75
108.42
39.29
62.22
92.56
PCR REAGENTS AND NUCLEOTIDES
............................................................................………………….. PC
Mfgr.
LTI
LTI
CLN
CLN
CLN
PEC
PEC
Mfgr No
18427-088
18427013
639102
639105
639505
N808-0246
N808-0240
Code
4010
5468
5985
5987
6051
5993
5382
Item Description
10 mM dNTP Mix
10mM dNTP Mix
Advantage cDNA PCR Kit
Advantage cDNA Plolymerase Mix
Advantage RT for PCR
AmpliTaq Gold™ w/ buffer I - 1000u
AmpliTaq Gold™ w/ buffer I - 250u
Unit
1 ML
100 µl
1 ea
1 ea
ea
1000u
250u
CC Price
$
216.22
$
28.61
$
139.34
$
254.83
$
241.50
$
284.65
$
86.08
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 71
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
AMR
BMB
PEC
NEN
NEN
BMB
AMR
BMB
PRO
AMR
BMB
AMR
BMB
PRO
AMR
BMB
AMR
BMB
USB
USB
USB
AMR
USB
SIG
PRO
AMR
BMB
AMR
BMB
AMR
AMR
DEN
AMR
PRO
BMB
N808-0244
N808-0248
N808-0242
N808-0247
N808-0241
N808-0245
N808-0249
N808-0243
N808-0171
N808-0152
N808-0160
N808-0155
N808-0166
N808-0172
N808-0161
N808-0156
N808-0167
N808-0153
N808-0150
N808-0100
27-2056-01
1140965
4303573
NEL542
NEL999
1093070
27-2066-01
1140922
U1201
27-2050-01
1051440
27-2050-02
1934511
U1221
27-2060-01
1051458
27-2060-02
1934520
70714
70716
70718
27-2045-01
70720
D7295-.5ml
U1211
27-2070-01
1051466
27-2070-02
1934538
27-2094-01
27-2035-02
CB-4420-2
27-2035-01
U1240
1969064
5997
5999
5995
5994
5383
5998
6000
5996
5263
5264
5212
5265
5266
5267
5213
5268
5269
5270
5251
5250
5343
4103
5407
5455
5456
3133
5345
4105
4241
5347
4106
5449
6044
4242
5348
4108
5450
6045
4110
4111
4112
5352
4114
5411
4243
5349
4115
5451
6046
5336
5353
3307
4125
4240
6043
AmpliTaq Gold™ w/ buffer I-12 pac (250u)
AmpliTaq Gold™ w/ buffer I-5 pac (1000u)
AmpliTaq Gold™ w/ buffer I-6 pac (250u)
AmpliTaq Gold™ w/ buffer II - 1000u
AmpliTaq Gold™ w/ buffer II - 250u
AmpliTaq Gold™ w/ buffer II-12 pac (250u)
AmpliTaq Gold™ w/ buffer II-5 pac (1000u)
AmpliTaq Gold™ w/ buffer II-6 pac (250u)
AmpliTaq® w/ buffer I - 1000u
AmpliTaq® w/ buffer I - 12 paq (250u)
AmpliTaq® w/ buffer I - 250u
AmpliTaq® w/ buffer I - 5 paq (1000u)
AmpliTaq® w/ buffer I - 6 paq (250u)
AmpliTaq® w/ buffer II - 1000u
AmpliTaq® w/ buffer II - 250u
AmpliTaq® w/ buffer II - 5 paq (1000u)
AmpliTaq® w/ buffer II - 6 paq (250u)
AmpliTaq® w/ buffer II- 12 paq (250u)
AmpliWax® PCR Gem 50
AmpliWax® PCR Gem 100
ATP 0.1M
ATP 0.1M
BigDye™ Terminator Kit
Biotin 11 CTP
Biotin 16 UTP
Biotin-16-dUTP
CTP 0.1M
CTP 0.1M
dATP (100mM)
dATP 0.1M
dATP 0.1M
dATP 100mM solution
DATP PCR Grade
dCTP (100mM)
dCTP 0.1M
dCTP 0.1M
dCTP 100mM solution
DCTP PCR Grade
ddATP Termination Mix
ddCTP Termination Mix
ddGTP Termination Mix
ddNTP Set
ddTTP Termination Mix
Deoxynucleotide Mix
dGTP (100mM)
dGTP 0.1M
dGTP 0.1M
dGTP 100mM solution
DGTP PCR Grade
DNA Polymerization Mix
dNTP Set 4x100umol
dNTP Set (4x25umol) 100mM
dNTP Set 0.1M
dNTP's (bulk) (100mM) 4x40umoles
dNTP's PCR Grade
3000u
5000u
1500u
1000u
250u
3000u
5000u
1500u
1000u
3000u
250u
5000u
1500u
1000u
250u
5000u
1500u
3000u
200 gem
200 gem
25 µmol
40 µmol
24 rxn
250nm
250nm
50 nmol
25 µmol
40 µmol
40 µmol
25 µmol
25 µmol
100 µm
ea
40 µmol
25 µmol
25 µmol
100 µm
ea
250 µl
250 µl
250 µl
4x0.5µmol
250 µl
1 vl
40 µmol
25 µmol
25 µmol
100 µm
ea
4x10umol
4x100µmol
EA
25 µmol
1 set
ea
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
1,032.97
1,413.18
516.49
284.65
86.08
1,032.97
1,413.18
516.49
288.37
900.62
75.05
1,431.72
450.32
288.37
75.05
1,431.72
450.32
900.62
73.50
73.50
48.04
63.54
192.37
187.07
186.03
231.41
48.04
63.54
61.74
47.25
47.07
146.49
47.07
61.74
47.25
47.07
146.49
47.07
25.20
25.20
25.20
107.11
25.20
25.61
61.74
47.25
47.07
146.49
47.07
83.48
519.80
130.45
159.88
193.18
165.51
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 72
PRO
AMR
BMB
AMR
BMB
BMB
BMB
BMB
BMB
BMB
BMB
BMB
BMB
BMB
PEC
PEC
PEC
PEC
PEC
PEC
PEC
PEC
AMR
STR
STR
QIA
QIA
FSH
FSH
FSH
SIG
BMB
BMB
FSH
FSH
FSH
FSH
AMR
USB
BMB
PRO
BMB
BMB
STR
STR
STR
STR
STR
STR
LTI
LTI
LTI
LTI
LTI
LTI
U1231
27-2080-01
1051482
27-2080-02
1934546
1732641
1732650
1759078
1681834
1759060
1681842
2032902
2032929
2140306
N808-0260
4303442
4303441
N808-0009
N808-0007
N808-0179
N808-0143
N808-0193
27-2076-01
600262
600260
203205
203203
E954140156
E954140164
E954140172
D5062-500u
2239264
3003230
E0032002250
E0032002404
E0032002501
E0032002552
27-2025-01
77102
1696513
PRG3161
1581295
1636138
600153
600154
600380
600250
600252
600320
11708-013
11708-021
11708-039
10790020
10966-018
10966-026
4244
5350
4127
5452
6047
5325
6011
6018
5274
6003
6012
5442
5443
5444
5404
5406
5405
5191
5247
5253
5202
5248
5344
6090
6089
6100
6099
5514
5515
5516
5410
5523
5524
5513
5510
5511
5512
5342
5241
5327
4199
4197
4289
5400
5401
4008
5402
5403
5447
6052
6053
6054
5459
6013
6014
dTTP (100mM)
dTTP 0.1M
dTTP 0.1M
dTTP 100mM solution
DTTP PCR Grade
Expand™ High Fidelity PCR System
Expand™ High Fidelity PCR System
Expand™ High Fidelity PCR System
Expand™ Long Template PCR System
Expand™ Long Template PCR System
Expand™ Long Template PCR System
FastStart Taq DNA Polymerase
FastStart Taq DNA Polymerase
GC Rich Polymerase
GeneAmp® dNTP blend 10mM
GeneAmp® dNTP blend 10mM-12 paq
GeneAmp® dNTP blend 10mM-6 paq
GeneAmp® Core Kit
GeneAmp® dNTP Set -10mM (4x3.2umol)
GeneAmp® EZ rTth RNA PCR Kit
GeneAmp® RNA PCR Core Kit
GeneAmp® XL PCR Kit
GTP 0.1M
Herculase Enhanced DNA Polymerase
Herculase Enhanced DNA Polymerase
Hot Star Taq (1000) with Q solution
Hot Star Taq (250) with Q solution
HotMaster Taq w/ buffer
HotMaster Taq w/ buffer
HotMaster Taq w/ buffer
Klentaq LA DNA Polymerase
LC FastStart DNA Mstr Sybrgreen
LC FastStart DNA Mstr Sybrgreen
MasterMix
MasterTaq Kit
MasterTaq Kit
MasterTaq Kit
NTP Set 0.1M
Nucleix-dATP set aq. (200uM) 0.1M
PCR Cleanup Kit
PCR Markers
PCR Nucleotide Mix
PCR Optimization Kit (for optimal pH & Mg)
Pfu DNA Polymerase
Pfu DNA Polymerase
Pfu Ultra High Fidelity
PfuTurbo™ DNA Polymerase
PfuTurbo™ DNA Polymerase
PfuTurbo™ HotStart DNA Polymerase
Platinum Pfx DNA Polymerase
Platinum Pfx DNA Polymerase
Platinum Pfx DNA Polymerase
Platinum Supermix Hi Fi
Platinum Taq DNA Polymerase - 100u
Platinum Taq DNA Polymerase - 250u
40 µmol
25 µmol
25 µmol
100 µm
ea
100u
500u
10x250u
100u
10x250u
500u
100u
500u
50 rxn
1 ml
12 ml
6 ml
100 rxn
1 set
100 rxn
100 rxn
100 rxn
25 µmol
500u
100u
(4x250u)
250u
100u
250u
1000u
500u
EA
EA
152u
100u
250u
500u
4x25µmol
200uM
100 rxn
250 µl
100 rxn
1 kit
100u
500u
100 u
100u
500u
100u
100 u
250 u
500 u
100 rxns
100u
250u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
61.74
42.53
47.07
146.49
47.07
69.03
324.75
1,455.11
73.74
1,546.10
342.01
59.62
283.18
146.69
29.40
352.80
176.41
164.21
36.50
355.64
237.21
375.40
48.04
295.62
68.03
261.24
92.42
31.95
74.55
266.25
162.58
828.35
207.09
39.94
22.37
55.91
106.50
159.88
148.99
111.39
54.12
49.42
123.15
85.98
345.80
102.99
93.54
382.65
104.88
81.62
185.54
350.35
121.70
46.05
110.99
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 73
LTI
LTI
QIA
SIG
PEC
PEC
PEC
LTI
LTI
PEC
PEC
SIG
QIA
QIA
SIG
PRO
BMB
BMB
BMB
LTI
LTI
PRO
PRO
FSH
FSH
SIG
BMB
PEC
PEC
SIG
BMB
BMB
BMB
BMB
BMB
AMR
NEB
NEB
10966-034
11304-011
204143
D4309-250u
N808-0178
N808-0098
N808-0180
10928-034
18064-071
4309155
4312704
S4438-100rxn
201203
201207
D1806-250u
M1868
1146165
NA
1435094
18038-018
18038-042
M1861
M1865
FB600010
FB600045
D4545-250u
1578553
4304437
4305719
T4809
3186172
3186202
3186199
1855476
1888382
27-2086-01
M0257S
M0254S
6015
5469
4634
6055
5255
5254
5249
5467
5465
4628
4629
5477
6001
6025
5408
5982
5185
5198
6002
5187
5188
5189
5258
5309
5311
5409
5199
4626
4627
5414
5461
5462
5463
5885
5988
5346
5257
5195
Platinum Taq DNA Polymerase - 500u
Platinum Taq High Fidelity
QuantiTect SYBR Green PCR Kit
Redtaq polymerase
rTth DNA Polymerase & EZ Buffer Pack
rTth DNA Polymerase with Buffer
rTth DNA Polymerase-XL w/XL Buffer Pack
SuperScript One Step RT-PCR with Platinum Taq
Superscript™ II Reverse Transcriptase
Sybr Green PCR Master Mix
Sybr Green PCR Master Mix
SYBR Green qPCR
Taq DNA Polymerase
Taq DNA Polymerase
Taq DNA Polymerase
Taq DNA Polymerase
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer A
Taq DNA Polymerase w/ buffer B
Taq DNA Polymerase w/10X Buffer
Taq PCR Core Kit
TaqMan Universal Master Mix
TaqMan Universal Master Mix
Taqstart Antibody
Tgo DNA Polymerase
Tgo DNA Polymerase
Tgo DNA Polymerase
TITAN™ One-Tube RT-PCR System
TITAN™ One-Tube RT-PCR System
UTP 0.1M
Vent® (exo-) Polymerase
Vent® DNA Polymerase
500u
100u
200 rxn
250 unit
100 rxn
100 rxn
100 rxn
25 rxn
4 x 10000u
pk
10 pk
100 rxn
250u
10x250u
250u
2500u
100u
250u
5000u
100u
500u
100u
500u
100u
100u
250u
100 rxn
200 rxn
2000 rxn
200 tst
50 rxns
100 rxns
250 rxns
100 rxn
25 rxn
25 µmol
200u
200u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
200.72
102.47
296.07
84.14
229.19
229.19
190.34
117.78
599.63
273.52
2,679.00
121.46
50.37
1,044.95
78.69
433.71
53.34
65.00
1,255.08
29.01
115.86
24.33
82.75
149.17
149.17
80.27
185.91
310.00
3,035.00
183.51
93.35
156.89
327.11
336.52
105.90
48.04
58.83
58.83
PRECAST GELS
......................................................................................................... PG
Mfgr.
INV
INV
INV
INV
INV
INV
INV
INV
INV
INV
INV
INV
Mfgr No
EA0375
EA03752
EA0378
EA0355
EA0358
NP0342
NP0335
NP0301
NP0302
NP0303
NP0341
NP0343
Code
6068
6069
6070
6071
6072
4013
4012
6073
6074
6075
6076
6077
Item Description
3-8% NuPAGE® Tris-Acetate Gels 1.0 mm 10 well
3-8% NuPAGE® Tris-Acetate Gels 1.0 mm 12 well
3-8% NuPAGE® Tris-Acetate Gels 1.5 mm 10 well
7% NuPAGE® Tris-Acetate Gels 1.0 mm 10 well
7% NuPAGE® Tris-Acetate Gels 1.5 mm 10 well
NuPAGE 12% Bis-Tris Gels 1.0mm 12 well
NuPAGE 4-12% Bis-Tris Gels 1.5mm 10 well
NuPAGE® 10% Bis-Tris Gels 1.0 mm 10 well
NuPAGE® 10% Bis-Tris Gels 1.0 mm 12 well
NuPAGE® 10% Bis-Tris Gels 1.0 mm 15 well
NuPAGE® 12% Bis-Tris Gels 1.0 mm 10 well
NuPAGE® 12% Bis-Tris Gels 1.0 mm 15 well
Unit
ea
ea
ea
ea
ea
ea
EA
ea
ea
ea
ea
ea
CC Price
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
$
9.73
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 74
INV
INV
INV
RAD
RAD
RAD
RAD
NP0321
NP0322
NP0323
1611101
1611155
1611104
1611158
6078
6079
6080
3340
3342
3341
3343
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 10 well
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 12 well
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 15 well
ReadyGel, 10% Tris-Hcl, 10 well
ReadyGel, 10% Tris-Hcl, 50ul
ReadyGel, 4-15% Tris-Hcl, 10 well
ReadyGel, 4-15% Tris-Hcl, 50ul
ea
ea
ea
EA
EA
EA
EA
$
$
$
$
$
$
$
9.73
9.73
9.73
8.14
8.14
8.14
8.14
RESTRICTION ENZYMES
...................................................................................................... RE
Mfgr.
NEB
NEB
NEB
NEB
NEB
LTI
NEB
BMB
NEB
PRO
NEB
NEB
BMB
NEB
NEB
NEB
AMR
BMB
LTI
NEB
NEB
PRO
NEB
BMB
NEB
PRO
BMB
LTI
NEB
NEB
PRO
BMB
NEB
NEB
NEB
BMB
NEB
PRO
NEB
NEB
BMB
NEB
PRO
Mfgr No
R0117S
R0161S
R0520S
R0541S
R0552S
45200-029
R0514S
899208
R0114S
R6361
R0558S
R0526S
814245
R0153S
R0174S
R0174L
27-0868-03
567604
15201-023
R0136S
R0136L
R6021
R0136T
693952
R0160S
R6071
348767
15213-010
R0144S
R0144M
R6081
1292307
R0540S
R0517S
R0199S
404233
R0162S
R6641
R0113S
R0524S
404217
R0197S
R6551
Code
6101
6104
6107
6312
6410
6108
6414
6346
6111
6396
6288
6323
6405
6116
6385
6416
6120
6117
6118
6119
6297
6121
6124
6126
6128
6398
6131
6281
6298
6408
6133
6407
6299
6387
6381
6348
6137
6400
6384
6388
6138
6139
6289
Item Description
Aat II
Acc I
Afl II
Afl III
Age I
Alu I
AlwN I
Apa I
Apa I
Apa I
Asc I
Ase I
Asp 718
Ava II
Avr II
Avr II
BamH I
BamH I
BamH I
BamH I
BamH I
BamH I
BamH I HC
Bcl I
Bcl I
Bgl I
Bgl II
Bgl II
Bgl II
Bgl II
Bgl II
Bsm I
BspE I
BspH I
BssH II
BstE II
BstE II
BstE II
BstX I
Bsu36 I
Cla I
Cla I
Cla I
Unit
500u
500u
2000u
250u
250u
500u
500u
5000u
2000u
5000u
500u
2000u
1000u
2000u
100u
500u
2000u
2500u
2000u
10000u
50000u
2500u
10000u
500u
3000u
1000u
500u
400u
2000u
10000u
500u
200u
1000u
500u
200u
500u
2000u
2000u
1000u
500u
500u
1000u
500u
CC Price
$
36.77
$
44.12
$
36.77
$
40.44
$
40.44
$
36.70
$
40.44
$
47.06
$
44.12
$
38.32
$
40.45
$
40.45
$
44.71
$
36.77
$
44.12
$
176.48
$
26.00
$
34.51
$
17.39
$
36.77
$
147.07
$
24.47
$
36.77
$
22.75
$
36.77
$
30.95
$
36.87
$
21.81
$
36.77
$
147.07
$
22.24
$
81.58
$
40.44
$
40.44
$
40.44
$
20.40
$
36.77
$
38.32
$
40.44
$
40.44
$
42.36
$
40.44
$
35.58
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 75
NEB
PRO
NEB
NEB
NEB
NEB
NEB
BMB
LTI
LTI
NEB
NEB
PRO
NEB
BMB
LTI
PRO
NEB
LTI
NEB
PRO
NEB
BMB
BMB
LTI
NEB
PRO
BMB
NEB
NEB
PRO
NEB
LTI
NEB
PRO
BMB
BMB
BMB
NEB
PRO
NEB
NEB
PRO
NEB
NEB
NEB
NEB
BMB
NEB
PRO
NEB
PRO
NEB
NEB
PRO
R0175S
R6291
R0176S
R0129S
R0510S
R0505S
R0521S
703737
15202-013
15202-021
R0101S
R0101L
R6011
R0101T
667145
15425-010
R6351
R0107S
25212-010
R0139S
R6441
R0103S
656313
656321
15207-012
R0104S
R6041
798983
R0155S
R0105S
R6301
R0171S
15232-036
R0142S
R6341
742945
822213
862495
R0147S
R6711
R0589S
R0198S
R6381
R0163S
R0534S
R0190S
R0191S
835315
R0193S
R6513
R0111S
R6801
R0131S
R0131L
R6501
6142
6401
6140
6389
6390
6143
6300
6144
6145
6336
6147
6301
6149
6152
6156
6157
6290
6159
6162
6325
6358
6166
6168
6354
6169
6303
6172
6174
6176
6180
6361
6182
6339
6185
6363
6184
6394
6417
6190
6364
6324
6123
6365
6413
6193
6305
6411
6199
6201
6203
6204
6367
6205
6308
6206
Dde I
Dde I
Dpn I
Dra I
Dra III
Eag I (Xma III)
EcoN I
EcoR I
EcoR I
EcoR I
EcoR I
EcoR I
EcoR I
EcoR I HC
EcoR V
EcoR V
EcoR V
Hae II
Hha I
Hha I
Hha I
Hinc II
Hind III
Hind III
Hind III
Hind III
Hind III
Hind III HC
Hinf I
Hpa I
Hpa I
Hpa II
Kpn I
Kpn I
Kpn I
Kpn I HC
Mae I
Mae II
Mbo I
Mbo I
Mfe I
Mlu I
Mlu I
Mnl I
Msc I
Nae I
Nar I
Nco I
Nco I
Nco I
Nde I
Nde I
Nhe I
Nhe I
Nhe I
500u
200u
1,000u
2000u
1000u
500u
1000u
5000u
5000u
20000u
10000u
50000u
5000u
10000u
2000u
2000u
2000u
2000u
1500u
2000u
1000u
1000u
5000u
10000u
5000u
10000u
5000u
10000u
5000u
500u
100u
2000u
5000u
3000u
2500u
2000u
50u
ea
500u
200u
500u
1000u
1000u
250u
100u
500u
200u
200u
1000u
200u
4000u
500u
1000u
5000u
250u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
36.77
17.81
40.44
40.44
40.44
40.44
40.44
25.89
15.35
66.32
36.77
147.07
17.80
36.77
36.08
28.10
31.14
36.77
49.45
36.77
26.53
36.77
25.10
40.01
14.06
36.77
14.09
40.01
36.77
36.77
29.48
36.77
37.25
36.77
33.17
32.95
76.09
224.35
44.12
36.12
44.13
40.36
35.58
40.44
40.44
36.77
36.77
54.91
36.77
48.92
40.44
66.34
40.44
161.78
40.77
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 76
BMB
LTI
NEB
NEB
PRO
NEB
PRO
NEB
NEB
NEB
BMB
NEB
PRO
AMR
BMB
PRO
BMB
NEB
PRO
NEB
BMB
NEB
PRO
NEB
PRO
BMB
NEB
NEB
PRO
NEB
NEB
PRO
BMB
PRO
BMB
NEB
BMB
LTI
NEB
PRO
NEB
PRO
BMB
NEB
PRO
BMB
LTI
NEB
PRO
NEB
PRO
LTI
NEB
PRO
NEB
1014706
15441-025
R0189S
R0189L
R6431
R0192S
R7091
R0127S
R0547S
R0560S
621625
R0140S
R6111
E1075Y
650137
R6321
642690
R0151S
R6331
R0167S
669792
R0156S
R6061
R0157S
R6221
348783
R0138S
R0138L
R6051
R0569S
R0169S
R6191
775258
R6211
1288016
R0123S
220566
15228-018
R0141S
R6121
R0130S
R6791
1008943
R0133L
R6591
1026950
15413-016
R0182S
R6261
R0132S
R6601
15222-011
R0187S
R6421
R0500S
6207
6208
6210
6309
6292
6213
6368
6214
6409
6412
6217
6220
6369
6223
6222
6370
6224
6227
6371
6230
6232
6234
6373
6314
6374
6235
6236
6315
6238
6322
6241
6375
6351
6243
6244
6246
6247
6286
6249
6251
6252
6376
6253
6254
6293
6255
6256
6258
6260
6392
6403
6261
6263
6377
6383
Not I
Not I
Not I
Not I
Not I
Nru I
Nru I
Nsi I
Pac I
Pme I
Pst I
Pst I
Pst I
Pvu I
Pvu I
Pvu I
Pvu II
Pvu II
Pvu II
Rsa I
Sac I (Sst I)
Sac I (Sst I)
Sac I (Sst I)
Sac II
Sac II
Sal I
Sal I
Sal I
Sal I
Sap I
Sau 3A I
Sau 3A I
Sca I
Sca I
Sfi I
Sfi I
Sma I
Sma I
Sma I
Sma I
SnaB I
SnaB I
Spe I
Spe I
Spe I
Sph I
Sph I
Sph I
Sph I
Ssp I
Ssp I
Sst I
Stu I
Stu I
Sty I
200u
200u
500u
2500u
200u
1000u
200u
1000u
250u
500u
3000u
10000u
3000u
150u
100u
100u
1000u
5000u
1000u
1000u
1000u
2000u
1000u
2000u
500u
500u
2000u
10000u
2000u
50u
200u
100u
500u
1000u
250u
2000u
1000u
1000u
2000u
1000u
500u
100u
200u
2500u
200u
200u
200u
250u
200u
1000u
500u
2000u
1000u
400u
3000u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
53.34
30.36
44.12
176.48
35.58
36.77
34.65
40.44
40.44
44.12
25.89
40.44
18.53
53.56
44.71
49.70
31.38
36.77
22.85
36.77
36.08
36.77
29.48
36.77
37.07
36.08
36.77
147.07
38.55
40.45
36.77
24.33
25.89
35.58
17.26
40.44
58.83
44.28
36.77
39.07
36.77
29.48
53.34
161.78
45.21
74.52
64.44
40.44
44.94
40.44
49.39
71.60
36.77
29.48
44.12
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 77
PRO
BMB
AMR
BMB
LTI
LTI
NEB
NEB
PRO
NEB
NEB
PRO
BMB
BMB
NEB
NEB
R6481
1371517
27-0948-01
674257
15226-012
15226-038
R0145S
R0145L
R6181
R0146S
R0146L
R6161
899194
703770
R0180S
R0194S
6404
6406
6272
6268
6269
6344
6271
6320
6379
6274
6287
6380
6352
6277
6393
6280
Sty I
Swa I
Xba I
Xba I
Xba I
Xba I
Xba I
Xba I
Xba I
Xho I
Xho I
Xho I
Xho I
Xho I HC
Xma I
Xmn I
2000u
200u
1500u
1000u
2000u
10000u
3000u
15000u
2000u
5000u
25000u
3000u
5000u
2500u
250u
1000u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
35.38
78.44
28.37
23.53
26.26
113.20
44.12
176.48
38.55
44.12
176.00
24.46
52.55
30.59
40.44
36.77
SERA ................................................................................................. SM
Mfgr.
SCL
GEM
LTI
EQU
SFB
BTI
HYC
LTI
HYC
Mfgr No
FBS-500
100125-500
26140-079
SFB30-1491
F2442-500
FBS-500
SH30071.03
16050-122
SH30087
Code
7138
7137
7141
7140
7135
7133
7139
7123
7126
Item Description
Fetal Bovine Serum
Fetal Bovine Serum, ES Qualified, Lot # A38507V
Fetal Bovine Serum, Lot # 1185797
Fetal Bovine Serum, Lot # SFB30-1491
Fetal Bovine Serum, lot #102K8410
Fetal Bovine Serum, lot #FBU2110
Fetal Bovine Serum, Lot# ANG19178
Horse Serum
Iron Enriched Calf Serum — Lot AKH12367
Unit
500 ML
500 ML
500ml
500ml
500 ml
500ML
500ml
500 ml
500 ml
CC Price
$
186.88
$
200.64
$
241.92
$
198.90
$
179.47
$
156.60
$
226.86
$
24.42
$
51.23
TISSUE CULTURE MEDIA AND
REAGENTS.........................................................................TC
Mfgr. Mfgr No
Code Item Description
COL CB40005A
1225
2.5S Nerve Growth Factor - Mouse Natural
LTI 12055-091
1228
AIM V Medium
LTI 12055-083
1229
AIM V Medium
LTI 15240-062
1167
Antibiotic-Antimycotic - Pen/Strep/Fung.
LTI 15240-096
1168
Antibiotic-Antimycotic - Pen/Strep/Fung.
CCS 1102
1102
Azaserine - 100X 0.6mM 0.1mg/ml
COL 354060
1220
bFGF - Human Recombinant
LTI 13150-016
1105
Cell Dissociation Buffer - Enzyme free
COL CB40236
1201
Collagen - Rat Tail
COL CB40235
1262
Dispase
CCS 1106
1106
DMEM - high glucose 25mM Hepes
LTI 11965-084
1107
DMEM - high glucose 4.5 gm/L
MED MT10013CV 1273
DMEM - high glucose 4.5 gm/L
MED MT10017CV 1274
DMEM - high glucose 4.5 gm/L - no sodium pyruvate
LTI 21063-029
1234
DMEM - high glucose w/o phenol red (1x)
LTI 11885-084
1109
DMEM - low glucose
LTI 10569-010
1195
DMEM H-glu w/ Glutamax
LTI 11320-033
1196
DMEM Nutrient Mix F12
Unit
CC Price
0.1 mg
$
500 ml
$
1L
$
100 ml
$
20 ml
$
10 ml
$
10 µg
$
100 ml
$
100 mg
$
100 ml
$
500 ml
$
1L
$
500 ml
$
500 ml
$
500 ml
$
500 ml
$
500ML
$
500 ML
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 78
139.85
32.70
62.22
8.20
3.75
12.00
114.00
13.19
127.06
73.74
10.00
5.96
3.98
3.98
24.61
3.82
4.31
15.23
LTI
MED
MED
LTI
LTI
LTI
BMB
LTI
BWH
LTI
MED
LTI
MED
BMB
COL
LTI
LTI
BMB
COL
LTI
CCS
LTI
MED
MED
MED
LTI
LTI
LTI
LTI
LTI
LTI
LTI
BWH
LTI
MED
CCS
LTI
CCS
LTI
MED
MED
CCS
CCS
BMB
MED
CCS
CCS
COL
BMB
COL
LTI
BMB
LTI
MED
COL
10566-016
MT10013CM
MT10017CM
11330-032
10459-014
14080-055
1666789
14200-075
BW17-513Q
14040-117
MT21030CM
14190-136
MT21031CM
855731
CB40001
11130-051
21127-022
1051407
CB40008EA
33016-015
1122
15290-018
MT61234RF
MT61234RG
MT30-234-CR
11811-031
11811-023
10131-035
15750-011
35050-061
11605-094
11550-043
BW12-615F
11765-047
MT10080CV
1134
14175-079
1137
14170-112
MT21020CM
MT21021CM
1139
1279
843555
MT30-240-CR
1140
1141
CB40043
1271164
CB40068
31980-030
1011456
12440-053
MT10016CV
354351
1298
1239
1260
1110
4523
1111
1112
1113
1240
1114
1241
1115
1243
1116
1219
1181
1199
1192
1224
1142
1122
1193
1264
1265
1282
1124
1125
1197
1126
1194
1182
1131
1244
1132
1245
1134
1231
1137
1296
1275
1276
1139
1279
1215
1283
1140
1141
1203
1269
1223
1297
1143
1147
1247
1204
DMEM w/GlutaMAX
500 ML
DMEM WITH L-GLUT; HI GLUCOSE; W/ NA PYRUVATE 1 L
DMEM WITH L-GLUT; HI GLUCOSE; W/O NA PYRUVATE 1 L
DMEM/F-12 - 1:1 mix w/ 15mM Hepes
500 ml
DMRIE-C
1 ml
DPBS (10X) with Ca++ & Mg++
500 ml
DPBS (10X) without Ca++ & Mg++
4L
DPBS (10X) without Ca++ & Mg++
500 ml
DPBS (1X) with Ca++ & Mg++
1L
DPBS (1X) with Ca++ & Mg++
1L
DPBS (1X) with Ca++ & Mg++
1L
DPBS (1X) without Ca++ & Mg++
1L
DPBS (1X) without Ca++ & Mg++
1L
Epidermal Growth Factor
100 ug
Epidermal Growth Factor - Culture Grade
0.1 mg
Essential Amino Acids - 50X MEM supplemt
100 ml
F12 Nutrient Mix, Kaighns Mod
500 ML
Fibronectin - Human
1 mg
Fibronectin - Human
1 mg
Fibronectin, human plasma
5mg
Freezing Mix - 95% Calf Serum 5% DMSO
100 ml
Fungizone
20ml
G418
1g
G418
5g
G418 Sulfate (50mg/ml)
20 ml
Geneticin (G418)®
5g
Geneticin (G418)®
1g
Geneticin® - (50mg/ml)
20 ml
Gentamicin
10 ml
Glutamax I, 100X
100ML
Grace's/Hink's supp - yeastolate+lactalbumin
500 ml
Ham's F-10
500 ml
Ham's F-12
500 ml
Ham's F-12
1L
Ham's F-12
500 ml
Hank's BSS (10X)
500 ml
Hank's BSS (1X) w/o Ca++ or Mg++ or phenol
1L
Hank's BSS 1X - w/20mM hepes/no Ca&Mg
1L
HBSS
500 ML
HBSS w/ ca and mg
1L
HBSS w/o ca and mg
1L
Hybridoma Growth Supp - low endotoxin 10X
25 ml
Hybridoma Growth Supplement - High Endotoxin 10–50X
25 ml
Hygromycin B (50mg/ml)
20 ml
Hygromycin B (50mg/ml)
20 ml
Hypoxanthine - 100X 10mM 1.36mg/ml
10 ml
Hypoxanthine/Thymidine - 100X 10mM/1.6mM
50 ml
IL-2 Human Recombinant
10000u
IL-2 Mouse Recombinant
10000u/1ml
IL-4 - Human Recombinant
5 µg
IMDM w/GlutaMAX
500 ML
Interleukin 2 - 10000u Human recombinant
1 ml
Iscove's Medium
500 ml
Iscove's Medium
500 ml
ITS Premix - Culture Supplement
5 ml
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 79
4.09
7.96
7.96
13.41
142.05
9.09
80.80
9.09
6.74
6.36
7.96
6.44
7.96
121.59
92.15
6.44
13.41
87.86
65.87
239.20
22.00
6.10
58.11
201.69
63.10
182.17
56.14
63.66
14.30
5.94
16.22
6.54
5.83
8.56
4.24
12.00
13.73
20.00
8.09
7.96
7.96
24.00
35.00
124.72
97.69
5.00
10.00
93.15
97.27
138.27
6.98
109.04
6.44
6.37
80.72
COL
CCS
CCS
CCS
COL
LTI
BWH
MED
COL
FSH
COL
COL
COL
LTI
LTI
BWH
LTI
MED
LTI
LTI
LTI
LTI
LTI
LTI
BMB
LTI
LTI
IGN
CCS
BWH
LTI
MED
LTI
LTI
BWH
LTI
MED
LTI
LTI
MED
LTI
LTI
LTI
LTI
LTI
CCS
MED
FSH
FSH
FSH
COL
COL
LTI
MED
LTI
354352
1148
1149
1191
CB40232EA
25030-081
BW17-605E
MT25005CI
354230
CB40234
CB40230
356237
354253
16600-082
11150-059
BW12-611Q
11095-072
MT10010CM
12571-048
12561-049
12561-056
17504-044
21103-049
11140-050
1011367
09400-72PF
31985-070
IG50 0615
1165
BW17-602E
15140-122
MT30002CI
12040-077
12040-093
BW12-702Q
11875-085
MT10040CM
11835-030
22400-089
MT10041CV
72400-047
15544-018
10902-096
10902-088
25080-094
1175
MT25-000-CI
BP2477-1
BP2476-1
BP2473-1
CB40054
CB40039
15090-046
MT25052CI
25300-054
1205
1148
1149
1191
1206
1187
1248
1249
1217
1207
1218
1216
1261
1183
1184
1250
1154
1251
1230
1155
1156
1209
1208
1158
1198
1190
1163
1214
1165
1252
1188
1253
1169
1170
1254
1171
1255
1226
1173
1256
1299
1210
1185
1277
1235
1175
1295
1286
1284
1285
1221
1263
1232
1259
1176
ITS+ Premix - Culture Supplement
Kanamycin - 100X - 10mg/ml
Kanamycin/Neomycin - 100X - 20 K/5 N mg/ml
Kennett's HY - 90% DMEM hg/10% NCTC 135
Laminin - Mouse
L-Glutamine - 100X - 200mM - 29.23mg/ml
L-Glutamine - 200mM
L-Glutamine - 200mM
Matrigel® Growth Factor Reduced
Matrigel® Matrix
Matrigel® Phenol Free & Growth Fact Reduced
Matrigel® Phenol Red Free
Matrisperse® Cell Recovery Solution
McCoy's 5A
Medium 199
MEM - w/ Earles' salts & L-Glut
MEM - w/ Earles' salts & L-glut
MEM - w/ Earles' salts & L-Glut
MEM alpha - w/ nucleosides
MEM alpha - w/o nucleosides
MEM alpha - w/o nucleosides
Neural Supplement - B27 (50X)
Neuralbasal Media
Non-essential amino acids - 100X
Nutridoma® SP
OPI 100X - 15mg O - 5 mg P - 20u I/ml
Opti-MEM® - reduced serum hybrid. medium
Origen® Hybridoma Cloning Factor - 10-50X
PEG(50%+5%DMSO) - hi efficiency/fusion
Pen/Strep - 100X -10000u/ml P - 10mg/ml S
Pen/Strep - 100X -10000u/ml P - 10mg/ml S
Pen/Strep - 100X -10mcg/ml P - 10mg/ml S
PFHM II - Protein Free Hybridoma Medium
PFHM II - Protein Free Hybridoma Medium
RPMI 1640
RPMI 1640
RPMI 1640
RPMI 1640 - no phenol
RPMI 1640 - w/ 25mM Hepes
RPMI 1640 - w/ 25mM Hepes
RPMI w/GlutaMAX
S.O.C. Transformation Media - 10x10 ml
SF900 II - Serum-free insect medium
SF900 II - Serum-free insect medium
Sodium Bicarbonate
Sodium Pyruvate - 100X 11g/l 100mM
Sodium Pyruvate, 100mM solution
TE 10X, pH 7.4
TE, 1X Solution, pH 7.4
TE, 1X Solution, pH 8.0
TGF-alpha - Human Recombinant
TGF-beta Human Natural
Trypsin - 2.5% (10X)
Trypsin-EDTA - 0.05% T - .53mM EDTA
Trypsin-EDTA - 0.05% T (1X)
20 ml
10 ml
25 ml
1L
1 mg
100 ml
100 ml
100 ml
10 ml
10 ml
10 ml
10 ml
100 ml
500 ml
500 ml
1L
1L
1L
1L
1L
500 ml
10 ml
500 ml
100 ml
10 ml
50 ml
500 ml
50 ml
2 ml
100 ml
100 ml
100 ml
1L
500 ml
1L
1L
1L
500 ml
500 ml
500 ml
500 ML
100 ml
500 ml
1L
100 ml
100 ml
100 ml
1L
1L
1L
50 µg
1 µg
100 ml
100 ml
100 ml
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 80
60.55
5.00
13.00
16.00
97.10
4.73
9.01
4.77
267.89
223.44
285.80
272.18
51.43
4.55
6.86
8.79
6.59
7.96
27.27
12.63
7.48
42.63
25.64
5.40
33.73
24.13
14.29
146.19
15.00
6.15
4.47
4.77
34.96
20.39
7.68
6.17
7.96
4.90
6.14
5.84
6.45
43.41
19.17
41.98
4.04
5.00
4.30
36.76
25.71
25.61
171.26
244.88
14.29
4.78
3.50
LTI
LTI
CCS
LTI
CCS
25200-056
15400-054
1178
11120-052
1180
1236
1177
1178
1179
1180
Trypsin-EDTA - 0.25% (1X)
Trypsin-EDTA - 0.5% (10X)
Versene - 0.02% EDTA in HBSS
Vitamin Mixture - 100X vitamin supplement
Water Sterile - Tissue Culture Grade
100 ml
100 ml
1L
100 ml
1L
$
$
$
$
$
STOCKED PRODUCTS: VENDOR LISTINGS
AMERSHAM BIOSCIENCES, INC.
......................................................................................................... AMR
_ Technical Services: (800) 526-3593
_ Website: http://www.amershambiosciences.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: All special orders for Amersham products must be received at the Cell Center by 2pm for next day
availability.
_ Special Conditions: All non-radioactive items in the Amersham catalog may be special ordered, no shipping or dry
ice fees.
_ Items ordered on Friday will be available on Monday.
_ Delivery: Overnight
PC
CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
MR 4484
27-4005-01
50 bp DNA Ladder
200 µg
$
154.36
CH 2340
27-6200-01
Acetyl CoA
10 mg
$
53.56
MS 4296
NAMP100
Amplify™
1L
$
143.86
IM
3186
27-4577-01
Anti-GST Antibody
0.5ml
$
207.13
IM
3143
NA931
Anti-Mouse Ig - HRP conjugated
1 ml
$
214.02
IM
3144
NA934
Anti-Rabbit Ig - HRP conjugated
1 ml
$
165.42
PC
5343
27-2056-01
ATP 0.1M
25 µmol
$
48.04
RE 6120
27-0868-03
BamH I
2000u
$
26.00
PC
5345
27-2066-01
CTP 0.1M
25 µmol
$
48.04
PC
5347
27-2050-01
dATP 0.1M
25 µmol
$
47.25
PC
5449
27-2050-02
dATP 100mM solution
100 µm
$
146.49
PC
5348
27-2060-01
dCTP 0.1M
25 µmol
$
47.25
PC
5450
27-2060-02
dCTP 100mM solution
100 µm
$
146.49
PC
5352
27-2045-01
ddNTP Set
4x0.5µmol
$
107.11
CH 2212
17-0340-01
Dextran Sulfate
100 g
$
119.29
PC
5349
27-2070-01
dGTP 0.1M
25 µmol
$
47.25
PC
5451
27-2070-02
dGTP 100mM solution
100 µm
$
146.49
PC
5336
27-2094-01
DNA Polymerization Mix
4x10umol
$
83.48
OE 5373
27-0514-01
DNase 1
1000u
$
63.79
PC
5353
27-2035-02
dNTP Set 4x100umol
4x100µmol
$
519.80
PC
4125
27-2035-01
dNTP Set 0.1M
25 µmol
$
159.88
PC
5350
27-2080-01
dTTP 0.1M
25 µmol
$
42.53
PC
5452
27-2080-02
dTTP 100mM solution
100 µm
$
146.49
NR 5415
RPN2132
ECL™ Plus Western Blotting Detection Reagents
1 ea
$
181.69
NR 3117
RPN2108
ECL™ Western Blot Analysis Starter System
1 kit
$
131.75
NR 3118
RPN2109
ECL™ Western Blotting Detection Reagents
1000cm2
$
103.33
NR 3119
RPN2106
ECL™ Western Blotting Detection Reagents
4000cm2
$
225.60
NR 3142
RPN2209
ECL™ Western Blotting Detection Reagents
2000cm2
$
162.74
OE 5287
E70073Y
Exonuclease I
2500u
$
81.94
CH 2126
17-1440-03
Ficoll-Paque® Plus solution
6x500ml
$
337.40
CH 2125
17-1440-02
Ficoll-Paque® solution
6x100ml
$
99.23
KT 5314
27-9261-01
First Strand cDNA Synthesis Kit
1 kit
$
207.13
CH 2213
17-0756-01
Glutathione Sepharose
10 ml
$
159.09
KT 5363
27-4590-01
GST Detection Module
50 rxn
$
182.72
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 81
5.48
6.27
19.00
6.84
9.00
KT
PC
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
OE
CO
CO
CO
CO
OE
CO
PC
CH
CO
KT
RE
MR
MR
MB
MB
KT
OE
MB
OE
OE
OE
OE
OE
PC
RE
5362
5344
2290
2333
2359
2139
2136
2137
2138
2358
2289
2357
2135
2292
2293
2356
2295
2308
4015
2286
2287
2143
5128
4467
4507
4509
4299
5131
4148
5342
2173
4297
2167
6223
4234
4235
4183
4239
4300
5375
5357
5289
5371
5174
5233
5368
5346
6272
27-4570-01
27-2076-01
RPN2020D
RPN303D
RPN137N
RPN203N
RPN2222N
RPN303N
RPN82N
RPN87N
RPN303E
RPN137B
RPN203B
RPN2020B
RPN303B
RPN87B
RPN2020F
RPN1645
RPN1677L
RPN2114K
RPN3114K
RPN1678K
27-0925-01
27-5325-01
27-5330-01
27-5120-01
27-5130-01
E2420Y
17-0855-02
27-2025-01
17-0891-01
27-5335-01
27-9558-01
E1075Y
RPN756
RPN755
27-2166-01
RPN1635
27-9240-01
RPN1633
27-0816-01
E70054Y
27-0808-01
27-0736-01
E2230Z
27-0846-01
27-2086-01
27-0948-01
GST Purifications Bulk Module
GTP 0.1M
Hybond™ ECL™ 20x20cm
Hybond™ ECL™ 30cmx3m
Hybond™ Nylon 137mm
Hybond™ Nylon 20cmx3m
Hybond™ Nylon 22x22cm
Hybond™ Nylon 30cmx3m
Hybond™ Nylon 82mm
Hybond™ Nylon 87mm
Hybond™-C extra sup nitrocellulose 30cmx3m
Hybond™-N+ charged nylon 137mm
Hybond™-N+ charged nylon 20cmx3m
Hybond™-N+ charged nylon 20x20cm
Hybond™-N+ charged nylon 30cmx3m
Hybond™-N+ charged nylon 87mm
Hybond™-PVDF 20x20cm
Hypercassette™ (beige) 35x43cm
Hyperfilm MP Enveloped, 8x10in
Hyperfilm™ ECL™ 8x10in
Hyperfilm™ ECL™ 8x10in
Hyperfilm™ MP 8x10in
Maloney-MLV Reverse Transcriptase
MicroSpin G-25 Columns
MicroSpin G-50 Columns
MicroSpin S-200 HR Columns
MicroSpin S-300 HR Columns
Mung Bean Nuclease
NICK® Columns
NTP Set 0.1M
Percoll®
ProbeQuant™ G-50 Micro Columns
PureTaq RTG PCR Beads
Pvu I
Rainbow Marker™ (14300-250000)
Rainbow Marker™ (2350-46000)
Random Primers (N)6
RapidHyb Hybridization™ Buffer
Ready-To-Go DNA Labelling Beads (-dCTP)
Rediprime™ kit for dCTP
RNAguard Ribonuclease Inhibitor
RNase H
SP6 RNA Polymerase
T4 Polynucleotide Kinase FPLCpure
Terminal Deoxy Transferase (TdT)
Thrombin Protease
UTP 0.1M
Xba I
5 prps
25 µmol
10 sht
1 roll
50/bx
1 roll
10/bx
1 roll
50/bx
50/bx
1 roll
50/bx
1 roll
10 sht
1 roll
50/bx
10 sht
1 ea
50 sheets
25 sht
75 sht
75 sht
200u
50 col
50 col
50 col
50 col
3000u
50 col
4x25µmol
1L
50 col
0.5ML
150u
250 µl
250 µl
50 Å260u
125 ml
1 kit
30 rxn
5000u
250u
3000u
200u
2500u
500u
25 µmol
1500u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
266.20
48.04
136.93
168.86
182.30
195.74
157.94
214.22
89.05
99.13
236.06
231.86
200.78
195.74
223.46
126.85
178.51
204.16
144.28
79.55
206.34
206.34
55.13
119.71
119.71
119.71
119.71
118.92
219.73
159.88
226.82
119.71
142.55
53.56
101.12
101.12
117.35
65.37
102.38
186.66
55.13
51.19
152.79
73.24
345.75
62.22
48.04
28.37
ROCHE DIAGNOSTICS .............................................................. BMB
_ Technical Services: (800) 262-4911
_ Website: http://www.roche-applied-science.com
_ All items listed below are available on site at the Cell Center.
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 82
_ Special Ordering: All items in the Roche catalog may be special ordered at a discount, no shipping or dry ice fees.
_ Conditions: All special orders for Roche products must be received at the Cell Center by 2pm for next day
availability.
_ Items ordered on Friday will be available on Monday.
_ Delivery: Overnight
PC CODE MFGR. NO. DESCRIPTION
SIZE
CC PRICE
KT 5519
1483188
1st Strand cDNA Kit
30 rxn
$
MB 4267
1204530
ABTS® Buffer
125 ml
$
MB 4266
1112422
ABTS® Tablets (50mg)
20 tab
$
CH 2102
101893
Acetyl CoA
10 mg
$
CH 2104
1685791
Acrylamide
500 g
$
OE 5101
1417215
Agarase
100u
$
OE 5374
1758250
Alkaline Phosphatase (shrimp)
1000u
$
OE 5103
109118
AMV Reverse Transcriptase
1000u
$
IM
3195
1583816
Anti-HA (12CA5)
200 µg
$
RE 6346
899208
Apa I
5000u
$
RE 6405
814245
Asp 718
1000u
$
PC
4103
1140965
ATP 0.1M
40 µmol
$
RE 6117
567604
BamH I
2500u
$
CH 2379
1383221
BCIP
3ml/150mg
$
RE 6126
693952
Bcl I
500u
$
CH 2303
874515
Bestatin
10 mg
$
RE 6131
348767
Bgl II
500u
$
PC
3133
1093070
Biotin-16-dUTP
50 nmol
$
CH 2114
1685830
Bis-Acrylamide
25 g
$
NR 3154
1096176
Blocking Reagent
50 g
$
MB 4104
711454
Bovine Serum Albumin (Nuclease free)
20 mg
$
RE 6407
1292307
Bsm I
200u
$
RE 6348
404233
BstE II
500u
$
OE 5108
713023
Calf Intestinal Alkaline Phosphatase
1000u
$
NR 3181
1644807
Cell Proliferation Kit (WST-1)
ea
$
CH 2115
757306
Cesium Chloride
1 kg
$
CH 2116
757292
Cesium Chloride
100 g
$
NR 3123
1500708
Chemiluminescent Western Blot Kit
1000cm2
$
RE 6138
404217
Cla I
500u
$
MB 4233
1213857
Collagenase P
100 mg
$
CH 5522
1873580
Complete EDTA Free 20 Tabs
EA
$
CH 5521
1836153
Complete Mini 25 Tabs
EA
$
PC
4105
1140922
CTP 0.1M
40 µmol
$
PC
4106
1051440
dATP 0.1M
25 µmol
$
PC
6044
1934511
DATP PCR Grade
ea
$
PC
4108
1051458
dCTP 0.1M
25 µmol
$
PC
6045
1934520
DCTP PCR Grade
ea
$
PC
4115
1051466
dGTP 0.1M
25 µmol
$
PC
6046
1934538
DGTP PCR Grade
ea
$
NR 3178
1585614
DIG/Genius High Prime DNA Labeling and Detection Starter Kit II 1 kit
$
NR 3175
1277073
Digoxigenin RNA Labeling Mix
20 rxn
$
NR 3151
1209256
Digoxigenin-11-UTP
250 nmol
$
CH 2122
100032
Dithiothreitol (DTT)
5g
$
MR 4480
1418009
DNA Marker IV (74bp - 19329bp)
50 µg
$
MR 4231
1449460
DNA Marker IX (72-1353bp)
50 µg
$
MR 4229
821705
DNA Marker V (8-587bp)
50 µg
$
MR 4230
1062590
DNA Marker VI (154-2176bp)
50 µg
$
MR 4545
1336045
DNA Marker VIII (19bp - 1114bp)
50 µg
$
MR 4232
1498037
DNA Marker X (75-12216bp)
100 µg
$
MR 4454
1721933
DNA Marker XIV (100 - 1500 bp) 100 bp ladder
50 µg
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 83
236.11
23.53
40.79
52.56
95.70
175.71
69.03
200.81
202.38
47.06
44.71
63.54
34.51
37.65
22.75
99.62
36.87
231.41
26.67
79.23
49.42
81.58
20.40
47.07
278.47
261.21
49.42
76.87
42.36
50.20
225.13
96.48
63.54
47.07
47.07
47.07
47.07
47.07
47.07
192.18
92.56
123.94
54.91
83.15
105.11
83.93
87.86
100.41
87.86
74.52
OE
OE
PC
MB
TC
PC
PC
RE
RE
TC
PC
PC
PC
PC
PC
PC
MB
PC
PC
TC
CH
MB
MB
PC
NR
CH
MB
CH
KT
KT
KT
CO
CO
RE
RE
RE
TC
TC
NR
TC
CH
OE
RE
PC
PC
CH
NR
NR
RE
RE
CH
RE
RE
TC
PC
5320
5114
6043
4126
1112
4127
6047
6144
6156
1116
5325
6011
6018
5274
6003
6012
5440
5442
5443
1192
2127
5421
5422
5444
3130
2131
4131
2134
5281
5282
5520
4512
4513
6168
6354
6174
1215
1269
3173
1143
2145
5125
6184
5523
5524
2146
6041
6042
6394
6417
2378
6199
6207
1198
5327
104159
776785
1969064
1202375
1666789
1051482
1934546
703737
667145
855731
1732641
1732650
1759078
1681834
1759060
1681842
3045595
2032902
2032929
1051407
1814320
1815091
1814443
2140306
1175025
100149
901393
1685929
1585584
1585592
1796828
1754777
1754785
656313
656321
798983
843555
1271164
1684795
1011456
724815
1008404
742945
2239264
3003230
1017128
2015200
2015196
822213
862495
1383213
835315
1014706
1011367
1696513
DNase I - Grade II
DNase I - RNase Free
dNTP's PCR Grade
DOTAP (Liposomal Transfection Reagent)
DPBS (10X) without Ca++ & Mg++
dTTP 0.1M
DTTP PCR Grade
EcoR I
EcoR V
Epidermal Growth Factor
Expand™ High Fidelity PCR System
Expand™ High Fidelity PCR System
Expand™ High Fidelity PCR System
Expand™ Long Template PCR System
Expand™ Long Template PCR System
Expand™ Long Template PCR System
ExtremeGENE (Transfection Reagent)
FastStart Taq DNA Polymerase
FastStart Taq DNA Polymerase
Fibronectin - Human
Formamide
FuGENE 6 (Nonliposomal Transfection Reagent)
FuGENE 6 (Nonliposomal Transfection Reagent)
GC Rich Polymerase
GENIUS™ 4 RNA Labeling Kit
Glycine
Glycogen
Guanidine Thiocyanate
High Prime DNA Labeling Kit
High Prime DNA Labeling mix for dCTP
High Pure PCR Template Prep
High Pure Plasmid Isolation Kit
High Pure Plasmid Isolation Kit
Hind III
Hind III
Hind III HC
Hygromycin B (50mg/ml)
IL-2 Mouse Recombinant
In Situ Cell Death Detection Kit - Fluorescein
Interleukin 2 - 10000u Human recombinant
IPTG
Klenow - Labeling grade
Kpn I HC
LC FastStart DNA Mstr Sybrgreen
LC FastStart DNA Mstr Sybrgreen
Leupeptin
Lumi Light
Lumi Light +
Mae I
Mae II
NBT (Nitro Blue Tetra)
Nco I
Not I
Nutridoma® SP
PCR Cleanup Kit
100 mg
10000u
ea
2 ml
4L
25 µmol
ea
5000u
2000u
100 ug
100u
500u
10x250u
100u
10x250u
500u
.4 ml
100u
500u
1 mg
500 ml
.4 ml
1 ml
50 rxn
2x10rxn
1 kg
20 mg
500 g
50 rxn
50 rxn
EA
50 col
250 col
5000u
10000u
10000u
20 ml
10000u/1ml
50 rxn
1 ml
1g
100u
2000u
EA
EA
25 mg
1 kit
1 kit
50u
ea
3 ml
200u
200u
10 ml
100 rxn
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 84
36.08
109.04
165.51
242.39
80.80
47.07
47.07
25.89
36.08
121.59
69.03
324.75
1,455.11
73.74
1,546.10
342.01
84.72
59.62
283.18
87.86
36.08
125.51
250.23
146.69
311.42
40.79
77.66
135.71
207.09
188.26
131.78
47.85
206.30
25.10
40.01
40.01
124.72
97.27
266.70
109.04
32.16
47.85
32.95
828.35
207.09
105.11
142.77
247.09
76.09
224.35
36.87
54.91
53.34
33.73
111.39
PC
PC
CH
CH
CH
CH
MB
CH
MB
CH
CH
CH
CH
RE
RE
RE
CO
CO
CO
CO
CO
KT
MB
OE
OE
OE
RE
RE
RE
RE
NR
RE
CH
RE
RE
CH
CH
RE
OE
OE
OE
OE
PC
PC
PC
PC
CH
PC
PC
PC
PC
PC
CH
CH
CH
4197
4289
2377
2172
2237
2174
4152
2345
5525
5136
2444
5518
5526
6217
6222
6224
4165
4166
4167
4326
4168
5139
4170
5143
5386
5388
6232
6235
6351
6244
3132
6247
2185
6253
6255
2186
2189
6406
5163
5167
5259
5171
5185
5198
6002
5199
2196
5461
5462
5463
5885
5988
2198
2199
2203
1581295
1636138
1429868
1359053
100995
1814303
108812
1697498
3335399
3115879
1373200
1000144
3115828
621625
650137
642690
1273922
1273990
1273965
1273973
1274015
1004760
1034731
1119915
109169
109193
669792
348783
775258
1288016
1093274
220566
1667262
1008943
1026950
1666681
100168
1371517
481220
1004786
1635379
709557
1146165
NA
1435094
1578553
100139
3186172
3186202
3186199
1855476
1888382
604205
604203
789704
PCR Nucleotide Mix
PCR Optimization Kit (for optimal pH & Mg)
Pefabloc® SC
Pepstatin (was #600160)
Phenol Saturated (Tris)
Phenol (was 100300)
Poly d(I-C)
Protease Inhibitor Cocktail Tablets (Complete)
Protector RNase Inhibitor
Proteinase K
Proteinase K
Proteinase K
Proteinase K Solution (20 mg/ml)
Pst I
Pvu I
Pvu II
Quick Spin™ G25 DNA
Quick Spin™ G25 RNA
Quick Spin™ G50 DNA
Quick Spin™ G50 DNA
Quick Spin™ G50 RNA
Random Primed DNA Labeling Kit
Random Primers (N)6
RNase - DNase free
Rnase A
RNase T1
Sac I (Sst I)
Sal I
Sca I
Sfi I
Sheep Anti-digoxigenin-AP
Sma I
Sodium Dodecyl Sulfate
Spe I
Sph I
SSC 20X
Sucrose
Swa I
T4 DNA Ligase
T4 DNA Polymerase
T4 DNA Rapid Ligation Kit (5 minutes)
T4 Polynucleotide Kinase
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq PCR Core Kit
TEMED
Tgo DNA Polymerase
Tgo DNA Polymerase
Tgo DNA Polymerase
TITAN™ One-Tube RT-PCR System
TITAN™ One-Tube RT-PCR System
Tris
Tris
Triton® X-100
100 rxn
1 kit
100 mg
10 mg
100 ml
500 g
10 Å260u
20 tabs
EA
100 mg
25 ml
500 mg
5 ml
3000u
100u
1000u
20 col
20 col
20 col
50 col
20 col
50 rxn
50 Å260u
500 µg
100 mg
100000u
1000u
500u
500u
250u
150u
1000u
100 g
200u
200u
4L
1 kg
200u
100u
100u
40 rxn
200u
100u
250u
5000u
100 rxn
50 g
50 rxns
100 rxns
250 rxns
100 rxn
25 rxn
1 kg
500 g
100 ml
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 85
49.42
123.15
90.99
47.07
48.63
92.56
66.68
225.13
64.32
59.61
313.77
61.97
90.99
25.89
44.71
31.38
71.38
103.54
71.38
133.35
103.54
209.44
111.39
57.26
57.26
33.73
36.08
36.08
25.89
17.26
127.86
58.83
29.81
53.34
74.52
80.80
32.16
78.44
40.79
73.74
104.33
160.81
53.34
65.00
1,255.08
185.91
32.16
93.35
156.89
327.11
336.52
105.90
57.26
36.08
25.10
OE
NR
NR
RE
CH
CH
RE
RE
5387
3180
3179
6268
2205
2206
6352
6277
109541
1767305
1767291
674257
745740
651745
899194
703770
tRNA
TUNEL Enzyme (optimized terminal transferase)
TUNEL Label (FITC dUTP + dNTPs)
Xba I
X-gal
X-gal
Xho I
Xho I HC
100 mg
1 ea
1 ea
1000u
1g
250 mg
5000u
2500u
$
$
$
$
$
$
$
$
BIOWHITTAKER MOLECULAR APPLICATIONS
............................................................……………………….. BMA
_ Technical Services: (800) 654-4452
_ Website: http://www.biowhittaker.com; http://www.bmaproducts.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: No.
_ Delivery: 3rd Business Day
PC
CODE MFGR. NO.
DESCRIPTION
TC
1240
BW17-513Q
DPBS (1X) with Ca++ & Mg++
TC
1244
BW12-615F
Ham's F-12
TC
1248
BW17-605E
L-Glutamine - 200mM
TC
1250
BW12-611Q
MEM - w/ Earles' salts & L-Glut
TC
1252
BW17-602E
Pen/Strep - 100X -10000u/ml P - 10mg/ml S
TC
1254
BW12-702Q
RPMI 1640
SIZE
1L
500 ml
100 ml
1L
100 ml
1L
CC PRICE
$
6.74
$
5.83
$
9.01
$
8.79
$
6.15
$
7.68
CAMBREX BIOSCEINCES
…………………………………………………………………….FMC
_ Technical Services: 800-341-1574
_ All items listed below are available on site at the Cell Center.
_ Special Orders: All items in the Cambrex catalog may be special ordered, no shipping or dry ice fees.
_ Conditions: All special orders must be received at the Cell Center by 2pm for next day availability.
_ Items ordered on Friday will be available on Wednesday.
_ Delivery: 3rd Business Day.
PC
CH
CH
CH
CH
CH
CH
CODE
2234
2263
2321
2233
2236
2235
MFGR. NO.
50100
50101
50090
50080
50004
50000
DESCRIPTION
Agarose (LMP) SeaPlaque®
Agarose (LMP) SeaPlaque®
Agarose NuSieve® 3:1
Agarose NuSieve® GTG®
Agarose SeaKem® LE
Agarose SeaKem® LE
SIZE
125 g
25 g
125 g
125 g
500 g
125 g
CC PRICE
$
289.24
$
105.93
$
223.98
$
278.01
$
285.54
$
111.25
CELL CENTER SERVICES
..................................................................................................... CC
_ Technical Services: (215) 898-2795
_ Website: http://www.med.upenn.edu/ccsf/
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: Please contact the Cell Center Service Facilty at 898-2795 for any special requests.
_ All items have been produced by the Cell Center, except for Xgal which is purchased from Diagnostic Chemicals,
Ltd. and is packaged under nitrogen. It is of high purity and quality and has been tested in screening assays and
histological staining procedures.
PC
CODE
DESCRIPTION
SIZE
CC PRICE
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 86
92.56
92.56
69.03
23.53
147.47
86.29
52.55
30.59
CH
2111
Ampicillin - 25mg/ml
10 ml
$
10.00
TC
1102
Azaserine - 100X 0.6mM 0.1mg/ml
10 ml
$
12.00
CH
2117
Chloramphenicol 34 mg/ml
10 ml
$
8.00
TC
1106
DMEM - high glucose 25mM Hepes
500 ml
$
10.00
TC
1122
Freezing Mix - 95% Calf Serum 5% DMSO
100 ml
$
22.00
TC
1134
Hank's BSS (10X)
500 ml
$
12.00
TC
1137
Hank's BSS 1X - w/20mM hepes/no Ca&Mg
1L
$
20.00
TC
1139
Hybridoma Growth Supp - low endotoxin 10X
25 ml
$
24.00
TC
1279
Hybridoma Growth Supplement - High Endotoxin 10–50X 25 ml
$
35.00
TC
1140
Hypoxanthine - 100X 10mM 1.36mg/ml
10 ml
$
5.00
TC
1141
Hypoxanthine/Thymidine - 100X 10mM/1.6mM
50 ml
$
10.00
TC
1148
Kanamycin - 100X - 10mg/ml
10 ml
$
5.00
TC
1149
Kanamycin/Neomycin - 100X - 20 K/5 N mg/ml
25 ml
$
13.00
TC
1191
Kennett's HY - 90% DMEM hg/10% NCTC 135
1L
$
16.00
CH
6005
LB + Ampicillin (50ug/ml) Agar Plates
10 pl
$
10.00
CH
6006
LB + Kanamycin (50ug/ml) Agar Plates
10 pl
$
10.00
CH
6007
LB + Tetracycline (20ug/ml) Agar Plates
10 pl
$
10.00
CH
6004
LB Agar Plates
10 pl
$
10.00
IM
3207
myc 1 - 9E10 antibody
0.5 mg
$
20.00
TC
1165
PEG(50%+5%DMSO) - hi efficiency/fusion
2 ml
$
15.00
MB
4463
Primer gt10 forward
5 µg
$
20.00
MB
4464
Primer gt10 reverse
5 µg
$
20.00
MB
4465
Primer gt11 forward
5 µg
$
20.00
MB
4466
Primer gt11 reverse
5 µg
$
20.00
MB
4190
Primer M13/pUC Forward 23mer
5 µg
$
20.00
MB
4191
Primer M13/pUC Reverse 23mer
5 µg
$
20.00
MB
4194
Primer Sp6 20mer
5 µg
$
20.00
MB
4192
Primer T3 20mer
5 µg
$
20.00
MB
4193
Primer T7 20mer
5 µg
$
20.00
MB
4524
Primer T7 Term
5 µg
$
20.00
TC
1175
Sodium Pyruvate - 100X 11g/l 100mM
100 ml
$
5.00
CH
2197
Tetracycline 12.5mg/ml
10 ml
$
8.00
TC
1178
Versene - 0.02% EDTA in HBSS
1L
$
19.00
TC
1180
Water Sterile - Tissue Culture Grade
1L
$
9.00
CH
2207
X-gal
1g
$
65.00
CLONTECH LABORATORIES
............................................................................................. CLN
_ Technical Services: 800-662-CLON
_ Website: http://www.clontech.com/
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: All items in the Clontech catalog may be special ordered, no shipping or dry ice fees.
_ Conditions: All special orders for CLN products must be received at the Cell Center by 2pm for next day availability.
_ Items ordered on Friday will be available on Tuesday.
_ Delivery: Next Day
PC
CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
PC
5985
639102
Advantage cDNA PCR Kit
1 ea
$
139.34
PC
5987
639105
Advantage cDNA Plolymerase Mix
1 ea
$
254.83
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 87
PC
KT
CO
CO
CH
CH
CH
CH
MB
CH
CH
MB
MB
MB
MB
6051
6008
4533
4536
5992
5989
5981
5991
6026
5905
5903
6027
6028
6030
6029
639505
631312
636055
636060
630415
630414
630413
630416
636831
630412
630411
635501
635506
630440
630439
Advantage RT for PCR
Cal Phos Mammalian Transfection Kit
CHROMA SPIN-10 Columns
CHROMA SPIN-100 Columns
DO Supplement-His
DO Supplement-Leu
DO Supplement-Trp
DO Supplement-Ura
Express Hyb
Minimal SD Agar Base
Minimal SD Base
Talon Metal Affinity Resin
Talon Superflow Metal Affinity Resin
Yeastmaker Carrier DNA
Yeastmaker Yeast Transformation System
ea
sm box
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
250 ml
1 ea
1 ea
10 ml
25 ml
ea
kit
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
241.50
143.63
112.10
112.12
34.29
37.07
34.29
34.29
148.63
139.00
92.66
69.64
194.59
199.23
203.86
SIZE
0.1 mg
10 µg
100 mg
100 ml
0.1 mg
1 mg
10000u
5 µg
5 ml
20 ml
1 mg
10 ml
10 ml
10 ml
100 ml
50 µg
1 µg
10µg
CC PRICE
$
139.85
$
114.00
$
127.06
$
73.74
$
92.15
$
65.87
$
93.15
$
138.27
$
80.72
$
60.55
$
97.10
$
267.89
$
285.80
$
272.18
$
51.43
$
171.26
$
244.88
$
225.88
COLLABORATIVE BIOMEDICAL PRODUCTS
................................................................... COL
_ Technical Services: (800) 343-2035
_ Website: http://www.bd.com/
_ All items listed below are available on site at the Cell Center.
_ Special Orders: No.
PC
CODE MFGR. NO.
DESCRIPTION
TC
1225
CB40005A
2.5S Nerve Growth Factor - Mouse Natural
TC
1220
354060
bFGF - Human Recombinant
TC
1201
CB40236
Collagen - Rat Tail
TC
1262
CB40235
Dispase
TC
1219
CB40001
Epidermal Growth Factor - Culture Grade
TC
1224
CB40008EA
Fibronectin - Human
TC
1203
CB40043
IL-2 Human Recombinant
TC
1223
CB40068
IL-4 - Human Recombinant
TC
1204
354351
ITS Premix - Culture Supplement
TC
1205
354352
ITS+ Premix - Culture Supplement
TC
1206
CB40232EA
Laminin - Mouse
TC
1217
354230
Matrigel® Growth Factor Reduced
TC
1218
CB40230
Matrigel® Phenol Free & Growth Fact Reduced
TC
1216
356237
Matrigel® Phenol Red Free
TC
1261
354253
Matrisperse® Cell Recovery Solution
TC
1221
CB40054
TGF-alpha - Human Recombinant
TC
1263
CB40039
TGF-beta Human Natural
IM
3208
CB40066
TNF-a
DENVILLE SCIENTIFIC (Q-BIOGENE / TKR)
........................................................................................................DEN
_ Technical Services: (800) 453-0385
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: Items in the Denville Scientific catalog may be special ordered at a discount, no shipping or
dry ice fees.
_ Conditions: Items listed in the Supplies and Chemicals sections of the Denville catalog may be special ordered.
_ Delivery: 3rd Day
PC
CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
MS 4462
R6651
Chillete personal tube cooler -12 tubes at -20C
1 ea
$
28.60
PC
3307
CB-4420-2
dNTP Set (4x25umol) 100mM
EA
$
130.45
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 88
CO
4614
1102-200
GeneClean Turbo
50 prp
$
57.36
CO
4450
1101-200
GeneClean® DNA Purification Spin Kit
50 prp
$
78.53
CO
4569
1101-400
GeneClean® DNA Purification Spin Kit
100 prp
$
139.44
CO
4269
1001-400
GeneClean® II DNA Purification Kit
300 prp
$
123.53
CO
4448
1001-600
GeneClean® III DNA Purification Kit
600 prp
$
180.01
CO
4451
2055-400
Lambda Quick! Phage DNA Isolation Spin Kit
25 prp
$
100.60
CH
2420
3002-031
LB Medium Capsules
1 kg
$
107.64
MS
4621
TKR-1301
Mini-Centrifuge
ea
$
250.00
MS
4497
C1201-CL
Mini-Centrifuge 6 x 1.5 ml
1 ea
$
185.59
MS
3327
P-1125
P-100 Filter Pipet Tips
EA
$
47.30
MS
3329
P-1123
P-1000 Filter Pipet Tips
EA
$
47.30
MS
3325
P-1096-FR
P2/P10 Eatra Long Filter Pipet Tip
EA
$
47.30
MS
3326
P-1121
P-20 Filter Pipet Tips
EA
$
47.30
MS
3328
P-1122
P-200 Filter Pipet Tips
EA
$
47.30
MS
4456
P3950-10
Pipet-mate Digital Pipette w/ tip ejector 10µl
1 ea
$
158.95
MS
4458
P3950-100
Pipet-mate Digital Pipette w/ tip ejector 100µl
1 ea
$
152.01
MS
4460
P3950-1000
Pipet-mate Digital Pipette w/ tip ejector 1000µl
1 ea
$
152.01
MS
4457
P3950-20
Pipet-mate Digital Pipette w/ tip ejector 20µl
1 ea
$
152.01
MS
4459
P3950-200
Pipet-mate Digital Pipette w/ tip ejector 200µl
1 ea
$
152.01
MS
4461
P3950-5000
Pipet-mate Digital Pipette w/ tip ejector 5000µl
1 ea
$
159.29
DIFCO LABORATORIES
(FISHER)........................................................................................DIF
_ Technical Services: (800) 343-2035
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 2nd Day
PC
CODE MFGR. NO.
DESCRIPTION
CH 5889
DF0145170
Agar - Granulated
CH 5890
DF0230173
Casamino Acids
CH 5891
DF0445174
LB Agar - Miller
CH 5893
DF0402170
LB Broth - Lennox
CH 5892
DF0446173
LB Broth - Miller
CH 5886
DF0118170
Peptone
CH 5887
DF0123173
Tryptone Peptone
CH 5888
DF0127179
Yeast Extract
SIZE
500 g
500 g
500 g
500 g
500 g
500 g
500 g
500 g
CC PRICE
$
59.09
$
51.84
$
44.71
$
20.43
$
20.43
$
41.30
$
36.39
$
51.68
ENZO LIFE SCIENCES, INC.
……………………………………………………………………….ENZ
_ Technical Services (800) 221-7705
_ All items below are available on site at the Cell Center.
_ Special Ordering: No.
_ Delivery: 2nd Day
PC
CODE MFGR. NO.
DESCRIPTION
KT 4630
42655-10
BioArray High Yield RNA Transcript Labeling Kit
KT 4631
42655-20
BioArray High Yield RNA Transcript Labeling Kit
SIZE
10 rxn
20 rxn
CC PRICE
$
705.00
$ 1,380.75
EPICENTRE TECHNOLOGIES
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 89
...............................................................................................EPI
_ Technical Services: (800) 284-8474
_ Web Site: http://www.epicentre.com
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 2nd Day
PC
CODE MFGR. NO.
DESCRIPTION
OE 5322
SL910K
SP6 RNA Polymerase (25u/µl)
SIZE
10000u
CC PRICE
$
102.02
FISHER SCIENTIFIC
............................................................................................................. FSH
_ Technical Services: (800) 533-8355
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 2nd Day
PC
CODE MFGR. NO.
DESCRIPTION
CH 2224
BP170500
Acrylamide
CH 4308
BP1423500
Agar - Granulated
MS 6023
121311
Aluminum Foil (18” x 50ft)
CH 4337
BP179100
Ammonium Persulfate
MS 4222
212363
ART® 10 aerosol resistant pipet tip (racked)
MS 4228
212362A
ART® 1000E aerosol resistant pipet tip (racked)
MS 4226
212368
ART® 100E aerosol resistant pipet tip (racked)
MS 4227
212361
ART® 200 aerosol resistant pipet tip (racked)
MS 4224
212365
ART® 20E aerosol resistant pipet tip (racked)
MS 4223
212364
ART® 20P aerosol resistant pipet tip (racked)
CH 2405
BP1610100
BCIP
CH 4338
BP17125
Bis-Acrylamide
CH 2406
BP168500
Boric Acid
CH 4309
BP1424500
Casamino Acids
CH 2226
BP210500
Cesium Chloride
FM 2118
57144
Chromatography Paper 3MM 46X57cm
CH 4339
BP339500
Citric Acid
CH 4440
BP1585100
Dextran Sulfate
CH 2239
BP1725
Dithiothreitol (DTT)
CH 2407
BP2311
DMSO
CH 2408
BP120500
EDTA Disodium Salt Dihydrate
CH 2227
BP227500
Formamide
CH 2230
BP3811
Glycine
CH 4442
BP221250
Guanidine Isothiocyanate
CH 4441
BP310100
HEPES
PC
5514
E954140156
HotMaster Taq w/ buffer
PC
5515
E954140164
HotMaster Taq w/ buffer
PC
5516
E954140172
HotMaster Taq w/ buffer
CH 2238
BP16201
IPTG
CH 5299
BP162010
IPTG
CH 4310
BP1425500
LB Agar - Miller
CH 4311
BP1427500
LB Broth - Lennox
CH 4312
BP1426500
LB Broth - Miller
CH 2410
BP214500
Magnesium Chloride
PC
5513
E0032002250 MasterMix
PC
5510
E0032002404 MasterTaq Kit
PC
5511
E0032002501 MasterTaq Kit
PC
5512
E0032002552 MasterTaq Kit
SIZE
500 g
500 g
ea
100 g
960/pk
800/pk
960/pk
960/pk
1000/pk
960/pk
100 mg
25 g
500 g
500 g
500 g
100/pk
500 g
100 g
5g
1L
500 g
500 ml
1 kg
250 g
100 g
100u
250u
1000u
1g
10 g
500 g
500 g
500 g
500 g
152u
100u
250u
500u
CC PRICE
$
74.59
$
68.93
$
8.53
$
15.24
$
57.00
$
57.00
$
57.00
$
57.00
$
56.98
$
57.00
$
18.65
$
19.44
$
17.59
$
46.88
$
131.63
$
85.24
$
15.72
$
87.37
$
42.03
$
30.36
$
30.64
$
30.90
$
29.35
$
58.61
$
32.36
$
31.95
$
74.55
$
266.25
$
27.17
$
202.45
$
54.23
$
26.88
$
26.94
$
22.37
$
39.94
$
22.37
$
55.91
$
106.50
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 90
TC
CH
CH
CH
CH
CH
CH
CH
CH
CH
MB
CH
CH
PC
PC
TC
TC
TC
CH
CH
CH
CH
CH
CH
CH
MS
CH
1207
4313
2231
5300
5301
5302
5303
2411
5211
5304
4201
2232
4443
5309
5311
1286
1284
1285
4444
5305
2412
2413
4314
2240
2409
6024
4315
CB40234
BP1420500
BP226500
BP1750I100
BP1750I400
BP1752I100
BP1752I400
BP366500
BP1700100
BP1700500
2123621
BP166500
BP2201
FB600010
FB600045
BP2477-1
BP2476-1
BP2473-1
BP1521
BP13381
BP152500
BP13071
BP1421500
BP169500
BP5611
15610
BP1422500
Matrigel® Matrix
Peptone
Phenol
Phenol Saturated pH 6.6
Phenol Saturated pH 6.6
Phenol/Chloroform/Isoamyl Alcohol
Phenol/Chloroform/Isoamyl Alcohol
Potassium Chloride
Proteinase K
Proteinase K
RNase AWAY
Sodium Dodecyl Sulfate (SDS)
Sucrose
Taq DNA Polymerase w/ buffer A
Taq DNA Polymerase w/ buffer B
TE 10X, pH 7.4
TE, 1X Solution, pH 7.4
TE, 1X Solution, pH 8.0
Tris Base
TRIS EDTA Solution 100X
Tris-Base
Tris-Glycine (10X)
Tryptone
Urea
Water(Sterile Nuclease Free)
Wrapping Film (18in x 1000ft)
Yeast Extract
10 ml
500 g
500 g
100 ml
400 ml
100 ml
400 ml
500 g
100 mg
500 mg
8.4 oz
500 g
1 kg
100u
100u
1L
1L
1L
1 kg
1L
500 g
1L
500 g
500 g
1L
ea
500 g
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
223.44
46.06
63.92
33.30
71.96
30.24
72.45
15.18
61.80
285.55
17.05
61.80
20.57
149.17
149.17
36.76
25.71
25.61
51.75
55.41
32.76
31.44
37.83
21.21
17.59
18.66
54.35
FUJI FILM
................................................................................................................FUJ
_ Technical Services: (800) 533-8355
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 2nd Day
PC
CODE MFGR. NO.
DESCRIPTION
FM 2325
04-441-118
Fuji RX Film NIF 14x17in
FM 2324
04-441-115
Fuji RX Film NIF 8x10in
SIZE
100 sht
100 sht
CC PRICE
$
305.28
$
111.69
GEMINI BIOPRODUCTS......................................................................................GEM
_ Technical Services: (800) 543-6464
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 2nd Business Day
PC
CODE MFGR. NO.
DESCRIPTION
SM 7137
100125-500
Fetal Bovine Serum, ES Qualified, Lot # A38507V
SIZE
500 ML
CC PRICE
$
200.64
HYCLONE LABORATORIES
......................................................................………..........................HYC
_ Technical Services: (800) 492-5663
_ All items listed below are available on site at the Cell Center.
_ Delivery: Overnight
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 91
PC
SM
SM
CODE MFGR. NO.
7139
SH30071.03
7126
SH30087
DESCRIPTION
Fetal Bovine Serum, Lot# ANG19178
Iron Enriched Calf Serum — Lot AKH12367
SIZE
500ml
500 ml
CC PRICE
$
226.86
$
51.23
IGEN INTERNATIONAL (FISHER)
........................................................................................ IGN
_ Technical Services: (800) 533-8355
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 2nd Day
PC
CODE MFGR. NO.
DESCRIPTION
TC
1214
IG50 0615
Origen® Hybridoma Cloning Factor - 10-50X
SIZE
50 ml
CC PRICE
$
146.19
INVITROGEN (GIBCO LIFE TECHNOLOGIES)
................................................................................. INV
_ Technical Services: (800) 955-6288
_ Web Site: http://www.invitrogen.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: All items in the Invitrogen / Life Technologies catalog may be special ordered at a discount, no
shipping or dry ice fees. Freezer items ordered on Friday will be available on Tuesday.
_ Delivery: Overnight
PC CODE MFGR. NO. DESCRIPTION
SIZE
CC PRICE
MR
4123 15615-016
1 kb DNA Ladder
250 µg
$
93.19
MR
4221
15615-024
1 kb DNA Ladder
1000 µg
$
309.87
MR
4302
10381-010
1 kb DNA Ladder - RTS - ready load
100 app
$
53.13
MR
4625
10787-018
1 kb Plus DNA Ladder
250 µg
$
84.88
PC
4010
18427-088
10 mM dNTP Mix
1 ML
$
216.22
MR
4101
15628-019
100 bp DNA Ladder
50 µg
$
41.70
MR
4304
10380-012
100 bp Ladder - RTS - ready load
100 app
$
64.05
MR
4196
10068-013
100 bp-2kb DNA Mass Ladder
200 µl
$
64.41
PC
5468
18427013
10mM dNTP Mix
100 µl
$
28.61
MR
4102
15613-011
123 Base Pair Ladder
100 µg
$
67.10
MR
4498
10597-011
25 bp DNA Ladder
50 µg
$
71.58
CH
5453
10812-014
2nd Strand Buffer
0.5 ml
$
53.89
PG
6068
EA0375
3-8% NuPAGE® Tris-Acetate Gels 1.0 mm 10 well
ea
$
9.73
PG
6069
EA03752
3-8% NuPAGE® Tris-Acetate Gels 1.0 mm 12 well
ea
$
9.73
PG
6070
EA0378
3-8% NuPAGE® Tris-Acetate Gels 1.5 mm 10 well
ea
$
9.73
MR
4499
10416-014
50 bp DNA Ladder
50 µg
$
71.58
PG
6071
EA0355
7% NuPAGE® Tris-Acetate Gels 1.0 mm 10 well
ea
$
9.73
PG
6072
EA0358
7% NuPAGE® Tris-Acetate Gels 1.5 mm 10 well
ea
$
9.73
CH
4185
30391-023
Agar - Bacterial Select
500 g
$
64.27
CH
2105
15510-019
Agarose
100 g
$
73.39
CH
2106
15510-027
Agarose
500 g
$
256.80
CH
1289
10975-035
Agarose 1000
100G
$
181.90
TC
1228
12055-091
AIM V Medium
500 ml
$
32.70
TC
1229
12055-083
AIM V Medium
1L
$
62.22
RE
6108
45200-029
Alu I
500u
$
36.70
TC
1167
15240-062
Antibiotic-Antimycotic - Pen/Strep/Fung.
100 ml
$
8.20
TC
1168
15240-096
Antibiotic-Antimycotic - Pen/Strep/Fung.
20 ml
$
3.75
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 92
IM
4636
R96025
Anti-V5 Antibody
50 ul
$
148.21
RE
6118
15201-023
BamH I
2000u
$
17.39
MR
4619
10748-010
BENCHMARK™ Prestained Protein Ladder
2 x 250u
$
78.71
RE
6281
15213-010
Bgl II
400u
$
21.81
CH
4635
R21001
Blasticidin
50 MG
$
140.80
MB
4618
15561-020
Bovine Serum Albumin, Acetylated
150 mg
$
98.67
TC
1105
13150-016
Cell Dissociation Buffer - Enzyme free
100 ml
$
13.19
CE
4118
18263-012
DH5alpha™ Library Efficiency Comp Cells
1 ml
$
94.88
CE
4119
18265-017
DH5alpha™ Subcloning Efficiency Comp Cells
2 ml
$
43.69
TC
1107
11965-084
DMEM - high glucose 4.5 gm/L
1L
$
5.96
TC
1234
21063-029
DMEM - high glucose w/o phenol red (1x)
500 ml
$
24.61
TC
1109
11885-084
DMEM - low glucose
500 ml
$
3.82
TC
1195
10569-010
DMEM H-glu w/ Glutamax
500ML
$
4.31
TC
1196
11320-033
DMEM Nutrient Mix F12
500 ML
$
15.23
TC
1298
10566-016
DMEM w/GlutaMAX
500 ML
$
4.09
TC
1110
11330-032
DMEM/F-12 - 1:1 mix w/ 15mM Hepes
500 ml
$
13.41
TC
4523
10459-014
DMRIE-C
1 ml
$
142.05
OE
5183
18010-025
DNA Polymerase I (E Coli)
1000U
$
113.64
OE
5113
18047-019
DNase I
20000u
$
184.22
OE
5225
18068-015
DNase I - Amplification Grade
100u
$
56.95
TC
1111
14080-055
DPBS (10X) with Ca++ & Mg++
500 ml
$
9.09
TC
1113
14200-075
DPBS (10X) without Ca++ & Mg++
500 ml
$
9.09
TC
1114
14040-117
DPBS (1X) with Ca++ & Mg++
1L
$
6.36
TC
1115
14190-136
DPBS (1X) without Ca++ & Mg++
1L
$
6.44
OE
5454
18052-019
E. Coli DNA Ligase
100u
$
23.20
RE
6145
15202-013
EcoR I
5000u
$
15.35
RE
6336
15202-021
EcoR I
20000u
$
66.32
RE
6157
15425-010
EcoR V
2000u
$
28.10
CH
2283
15575-020
EDTA 0.5M (4 x 100ml)
400 ml
$
23.91
CE
5423
18290-015
ElectroMAX DH10B Efficiency Comp Cells
5x.1ml
$
156.66
CE
5426
11319-019
ElectroMAX DH5alpha-E Efficiency Comp Cells
5x.1ml
$
154.63
MS
4519
P410-50
Electroporation Cuvettes
0.1cm
$
115.26
MS
4520
P450-50
Electroporation Cuvettes
0.2cm
$
115.26
MS
4521
P460-50
Electroporation Cuvettes
0.4cm
$
115.26
OE
5475
10480-010
Elongase Enzyme Mix
100 rxn
$
100.62
TC
1181
11130-051
Essential Amino Acids - 50X MEM supplemt
100 ml
$
6.44
CH
2277
15585-011
Ethidium Bromide (ETBR) 10mg/ml
10 ml
$
28.47
TC
1199
21127-022
F12 Nutrient Mix, Kaighns Mod
500 ML
$
13.41
CO
5221
K1593-02
FastTrack® 2.0 Kit
6 rxn
$
322.10
SM
7141
26140-079
Fetal Bovine Serum, Lot # 1185797
500ml
$
241.92
TC
1142
33016-015
Fibronectin, human plasma
5mg
$
239.20
TC
1193
15290-018
Fungizone
20ml
$
6.10
TC
1124
11811-031
Geneticin (G418)®
5g
$
182.17
TC
1125
11811-023
Geneticin (G418)®
1g
$
56.14
TC
1197
10131-035
Geneticin® - (50mg/ml)
20 ml
$
63.66
TC
1126
15750-011
Gentamicin
10 ml
$
14.30
TC
1194
35050-061
Glutamax I, 100X
100ML
$
5.94
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 93
CH
2130
15514-011
Glycerol
500 ml
$
18.18
CH
2132
15527-013
Glycine
500 g
$
23.53
TC
1182
11605-094
Grace's/Hink's supp - yeastolate+lactalbumin
500 ml
$
16.22
CH
2133
15502-016
Guanidine HCL
500 g
$
65.42
TC
1131
11550-043
Ham's F-10
500 ml
$
6.54
TC
1132
11765-047
Ham's F-12
1L
$
8.56
TC
1231
14175-079
Hank's BSS (1X) w/o Ca++ or Mg++ or phenol
1L
$
13.73
TC
1296
14170-112
HBSS
500 ML
$
8.09
CH
4445
15630-080
HEPES Buffer Solution (1M)
100 ml
$
26.77
RE
6162
25212-010
Hha I
1500u
$
49.45
RE
6169
15207-012
Hind III
5000u
$
14.06
SM
7123
16050-122
Horse Serum
500 ml
$
24.42
MB
4135
15279-011
Human Cot-1® DNA
500 µg
$
108.53
TC
1297
31980-030
IMDM w/GlutaMAX
500 ML
$
6.98
CH
6092
Q602-20
imMedia™ Amp Blue
ea
$
225.64
CH
6094
Q612-20
imMedia™ Kan Blue
ea
$
177.96
CH
6095
Q621-20
imMedia™ Zeo Agar
ea
$
276.96
TC
1147
12440-053
Iscove's Medium
500 ml
$
6.44
OE
5120
18012-021
Klenow - Sequencing Grade
100u
$
39.22
RE
6339
15232-036
Kpn I
5000u
$
37.25
MR
4136
15612-013
Lambda DNA/HindIII Fragment
500 µg
$
90.41
MR
4307
10382-018
Lambda DNA/HindIII Fragment - RTS - ready load
100 app
$
44.63
CH
4186
22700-025
LB Agar
500 g
$
47.15
TC
1187
25030-081
L-Glutamine - 100X - 200mM - 29.23mg/ml
100 ml
$
4.73
MB
6031
11668-019
LipofectAMINE 2000
1.5ml
$
273.82
MB
6032
11668-027
LipofectAMINE 2000
0.75ml
$
166.04
MB
4181
18324-012
Lipofectamine™
1 ml
$
165.28
MB
4582
18324-020
Lipofectamine™
4 x 1 ml
$
543.86
MB
4624
18324-111
Lipofectamine™
.5 ml
$
98.67
MB
4138
18292-011
Lipofectin® Reagent
1 ml
$
125.82
MR
5182
LC5600
MagicMark Western Protein Standard
250ul
$
177.71
MR
4014
LC5602
MagicMark XP
250ul
$
178.10
OE
5127
28025-013
Maloney-MLV Reverse Transcriptase
40000u
$
141.01
MR
4596
LC5677
Mark12™ Wide Range Molecular Weight Standard
1.0 ml
$
92.63
CE
5458
12034-013
Max Efficiency DH5@T1 Competent Cells
1 ml (5 x 0.2ml) $
123.50
CE
5425
18258-012
MAX Efficiency DH5alpha Efficiency Comp Cells
5x.1ml
$
157.71
CE
5474
10268-019
Max Efficiency STBL2
1 ML
$
148.43
TC
1183
16600-082
McCoy's 5A
500 ml
$
4.55
TC
1184
11150-059
Medium 199
500 ml
$
6.86
TC
1154
11095-072
MEM - w/ Earles' salts & L-glut
1L
$
6.59
TC
1230
12571-048
MEM alpha - w/ nucleosides
1L
$
27.27
TC
1155
12561-049
MEM alpha - w/o nucleosides
1L
$
12.63
TC
1156
12561-056
MEM alpha - w/o nucleosides
500 ml
$
7.48
CO
5220
K1520-02
Micro-FastTrack™ Kit
20 rxn
$
369.52
MR
4597
LC5725
MultiMark® Multi-Colored Standard
500 µl
$
106.52
MB
4620
17502-048
N2 Supplement
5 ml
$
38.68
TC
1209
17504-044
Neural Supplement - B27 (50X)
10 ml
$
42.63
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 94
TC
1208
21103-049
Neuralbasal Media
500 ml
$
25.64
KT
5133
FM
2441
18160-010
Nick translation Kit
50 rxn
$
128.09
LC2000
Nitrocellulose Membrane (0.2 µm) Filter Paper Sandwich
20/pk
$
104.21
FM
TC
2442
LC2001
Nitrocellulose Membrane (0.45 µm) Filter Paper Sandwich
20/pk
$
104.21
1158
11140-050
Non-essential amino acids - 100X
100 ml
$
RE
5.40
6208
15441-025
Not I
200u
$
30.36
CH
4011
NP00061
NP Transfer Buffer (20X)
1L
$
100.04
PG
4013
NP0342
NuPAGE 12% Bis-Tris Gels 1.0mm 12 well
ea
$
9.73
PG
4012
NP0335
NuPAGE 4-12% Bis-Tris Gels 1.5mm 10 well
EA
$
9.73
PG
6073
NP0301
NuPAGE® 10% Bis-Tris Gels 1.0 mm 10 well
ea
$
9.73
PG
6074
NP0302
NuPAGE® 10% Bis-Tris Gels 1.0 mm 12 well
ea
$
9.73
PG
6075
NP0303
NuPAGE® 10% Bis-Tris Gels 1.0 mm 15 well
ea
$
9.73
PG
6076
NP0341
NuPAGE® 12% Bis-Tris Gels 1.0 mm 10 well
ea
$
9.73
PG
6077
NP0343
NuPAGE® 12% Bis-Tris Gels 1.0 mm 15 well
ea
$
9.73
PG
6078
NP0321
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 10 well
ea
$
9.73
PG
6079
NP0322
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 12 well
ea
$
9.73
PG
6080
NP0323
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 15 well
ea
$
9.73
CH
6081
NP0005
NuPAGE® Antioxidant
15ml
$
15.28
CH
6082
NP0007
NuPAGE® LDS Sample Buffer (4X)
10ml
$
7.64
CH
6083
NP0002
NuPAGE® MES SDS Running Buffer (20X)
500ml
$
43.07
CH
6084
NP0001
NuPAGE® MOPS SDS Running Buffer (20X)
500ml
$
43.07
CH
6085
NP0004
NuPAGE® Sample Reducing Agent (10X)
250ul
$
7.64
CH
6086
NP0006
NuPAGE® Transfer Buffer (20X)
125ml
$
13.43
CH
6087
LA0041
NuPAGE® Tris-Acetate SDS Running Buffer (20X)
500ml
$
41.22
MB
6125
18418-012
Oligo (dT) 12-18 Primer
25 UG
$
45.40
MB
4009
12252-011
Oligofectamine Reagent
1 ML
$
188.12
CE
4637
C862003
One Shot MACH 1 Comp eColi (LTI Shelf)
20 rxn
$
235.31
CE
4622
12297-016
One Shot® MAX Efficiency® DH5a®-T1®
20 x 50ul
$
243.98
CE
4334
C2020-03
OneShot™ INVaF' Competent Cells
21x50µl
$
243.61
CE
5445
C4040-03
OneShot™ TOP10 Competent Cells
21x50µl
$
238.08
CE
4335
C3030-03
OneShot™ TOP10F' Competent Cells
21x50µl
$
235.31
TC
1190
09400-72PF
OPI 100X - 15mg O - 5 mg P - 20u I/ml
50 ml
$
24.13
TC
1163
31985-070
Opti-MEM® - reduced serum hybrid. medium
500 ml
$
14.29
KT
6098
K4900-01
pcDNA3.1© Directional TOPO® Expression Kit (Top 10 Cells) 20 rxn
$
360.57
TC
1188
15140-122
Pen/Strep - 100X -10000u/ml P - 10mg/ml S
100 ml
$
4.47
TC
1169
12040-077
PFHM II - Protein Free Hybridoma Medium
1L
$
34.96
TC
1170
12040-093
PFHM II - Protein Free Hybridoma Medium
500 ml
$
20.39
CH
2176
15513-039
Phenol Saturated (Buffer)
100 ml
$
58.24
MR
4151
15611-015
Phi-X174 DNA/Hae III Fragment
40 µg
$
54.30
MR
4305
10383-016
Phi-X174 DNA/Hae III Fragment - RTS - ready load
100 app
$
83.60
PC
6052
11708-013
Platinum Pfx DNA Polymerase
100 u
$
81.62
PC
6053
11708-021
Platinum Pfx DNA Polymerase
250 u
$
185.54
PC
6054
11708-039
Platinum Pfx DNA Polymerase
500 u
$
350.35
PC
5459
10790020
Platinum Supermix Hi Fi
100 rxns
$
121.70
PC
6013
10966-018
Platinum Taq DNA Polymerase - 100u
100u
$
46.05
PC
6014
10966-026
Platinum Taq DNA Polymerase - 250u
250u
$
110.99
PC
6015
10966-034
Platinum Taq DNA Polymerase - 500u
500u
$
200.72
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 95
PC
5469
11304-011
Platinum Taq High Fidelity
100u
$
MB
6034
11514-015
Plus Reagent
.85 ml
$
87.73
IM
3111
15918-014
Protein A Agarose
5 ml
$
166.69
IM
3112
15920-010
Protein G Agarose
5 ml
$
253.01
FM
2443
LC2002
PVDF Membrane/Filter Paper Sandwich
20/pk
$
101.89
KT
5227
18428-011
Rad Prime Labeling Kit
30 rxn
$
128.88
MR
4632
12308-011
Ready Load 1KB Plus DNA Ladder
100 rxn
$
73.79
OE
5141
18021-014
Ribonuclease H
30u
$
73.88
MR
4172
15620-016
RNA Ladder 0.24-9.5 kb
75 µg
$
80.29
TC
1171
11875-085
RPMI 1640
1L
$
6.17
TC
1226
11835-030
RPMI 1640 - no phenol
500 ml
$
4.90
TC
1173
22400-089
RPMI 1640 - w/ 25mM Hepes
500 ml
$
6.14
TC
1299
72400-047
RPMI w/GlutaMAX
500 ML
$
6.45
TC
1210
15544-018
S.O.C. Transformation Media - 10x10 ml
100 ml
$
43.41
CH
2284
15553-027
SDS 10% (4x100ml)
400 ml
$
25.51
MR
4599
LC5925
SeeBlue® Plus2 Pre-Stained Standard
500 µl
$
99.11
MR
4598
LC5625
SeeBlue® Pre-Stained Standard
500 µl
$
99.11
TC
1185
10902-096
SF900 II - Serum-free insect medium
500 ml
$
19.17
TC
1277
10902-088
SF900 II - Serum-free insect medium
1L
$
41.98
MR
4600
LC6060
SimplyBlue™ SafeStain
1L
$
79.66
RE
6286
15228-018
Sma I
1000u
$
44.28
TC
1235
25080-094
Sodium Bicarbonate
100 ml
$
4.04
OE
5156
18018-010
Sp6 RNA Polymerase
500u
$
48.07
RE
6256
15413-016
Sph I
200u
$
64.44
CH
2279
15557-036
SSC 20X
4L
$
49.53
CH
2285
15591-035
SSPE 20X
4L
$
79.75
RE
6261
15222-011
Sst I
2000u
$
71.60
IM
3116
19543-024
Streptavidin Red 670™
100 µg
$
157.50
OE
5460
11917010
Superscript Custom Choice Kit
10 rxn
$
656.60
KT
5226
11904-018
Superscript First-Strand Synthesis System for RT-PCR
50 rxn
$
235.30
OE
5478
18080044
Superscript III Reverse Transcriptase
10000u
$
158.13
OE
5476
12574-026
SuperScript III, 1 step w/Platinum Taq 100
100 rxn
$
351.55
PC
5467
10928-034
SuperScript One Step RT-PCR with Platinum Taq
25 rxn
$
117.78
OE
5159
18064-014
Superscript™ II Reverse Transcriptase
10000u
$
149.91
PC
5465
18064-071
Superscript™ II Reverse Transcriptase
4 x 10000u
$
599.63
OE
5160
18053-017
Superscript™ Reverse Transcriptase
10000u
$
154.90
OE
5164
15224-017
T4 DNA Ligase
100u
$
26.09
OE
5229
15224-025
T4 DNA Ligase
500u
$
115.12
OE
5168
18005-017
T4 DNA Polymerase
50u
$
31.89
OE
5464
18005-025
T4 DNA Polymerase
250u
$
103.79
OE
5172
18004-010
T4 Polynucleotide Kinase
200u
$
50.03
KT
5389
K2050-01
TA Cloning Kit® with Dual Promoters (INVaF')
20 rxn
$
289.93
CH
2275
15558-026
TAE 10X
4L
$
58.24
PC
5187
18038-018
Taq DNA Polymerase w/ buffer
100u
$
29.01
PC
5188
18038-042
Taq DNA Polymerase w/ buffer
500u
$
115.86
OE
5194
10533-065
Terminal Deoxy Transferase (TdT)
500u
$
108.42
KT
6097
K4500-01
TOPO TA Cloning® Kit (TOP10 Cells)
20 rxn
$
322.35
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 96
102.47
KT
5397
K4550-01
TOPO TA Cloning® Kit (TOP10F' Cells)
20 rxns
$
308.67
KT
5466
K4520-01
TOPO TA Cloning® Kit (with LTI 4622 - DH5a Cells)
20 rxns
$
319.57
KT
5398
K4650-01
TOPO TA Cloning® Kit Dual Promoter (TOP10F' Cells)
1 ea
$
322.99
KT
5446
K4575-01
TOPO TA Cloning® Kit for Sequencing (Top 10 Cells)
20 rxn
$
334.39
CH
2281
15567-027
TRIS pH 7.5
1L
$
17.73
CH
2282
15568-025
TRIS pH 8.0
1L
$
19.28
CH
2202
15506-017
Tris Hydrochloride
500 g
$
50.10
CH
5457
15596018
Trizol
200 ml
$
149.82
MB
4182
15596-026
TRIzol Reagent
100 ml
$
78.80
TC
1232
15090-046
Trypsin - 2.5% (10X)
100 ml
$
14.29
TC
1176
25300-054
Trypsin-EDTA - 0.05% T (1X)
100 ml
$
3.50
TC
1236
25200-056
Trypsin-EDTA - 0.25% (1X)
100 ml
$
5.48
TC
1177
15400-054
Trypsin-EDTA - 0.5% (10X)
100 ml
$
6.27
CH
2204
15505-050
Urea
2 kg
$
67.36
TC
1179
11120-052
Vitamin Mixture - 100X vitamin supplement
100 ml
$
6.84
RE
6269
15226-012
Xba I
2000u
$
26.26
RE
6344
15226-038
Xba I
10000u
$
113.20
MB
4180
15401-011
Yeast tRNA
25 mg
$
44.91
CH
4189
22712-020
YT Broth 2X
500 g
$
33.58
MB
5417
R250-01
Zeocin
1g
$
175.07
KT
6096
K2800-20
Zero Blunt® TOPO® PCR Cloning Kit (TOP 10 Cells)
20 rxn
$
338.20
KODAK
.............................................................................................................KOD
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: Overnight
PC
CODE MFGR. NO.
DESCRIPTION
FM 2306
V8701302
Biomax™ Film MR-1 8x10in
FM 2307
V8715187
Biomax™ Film MR-1 35x43cm
FM 2364
V8294985
Biomax™ Film MS-1 8x10in
FM 2382
EB135-24
Elite II Slide Film 100 - 24 Exposures
FM 2383
EB135-36
Elite II Slide Film 100 - 36 Exposures
FM 2331
V1651678
X-Omat™ AR-2 interleaved film 35x43cm
FM 2332
V1651579
X-Omat™ AR-2 interleaved film 8x10in
FM 2211
V1651454
X-Omat™ AR-5 non-interleaved film 8x10in
SIZE
50 sht
50 sht
50 sht
1 roll
1 roll
50 sht
50 sht
50 sht
CC PRICE
$
138.93
$
300.20
$
118.70
$
6.76
$
9.13
$
353.46
$
182.70
$
144.05
MARLIGEN BIOSCIENCES, INC.
………………………………………………………………………MLG
_ Technical Services: (866) 464-4990
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: No
_ Delivery: 2nd Business Day
PC CODE MFGR. NO.
DESCRIPTION
KT 5622
11452-018
High Pure Maxi Kit
KT 5625
11456-019
Rapid Gel Extraction Kit
KT 5624
11458-015
Rapid PCR Purification Kit
KT 5623
11453-016
Rapid Plasmid Mini Prep
SIZE
10rxn
50rxn
50rxn
50rxn
CC PRICE.
$
122.65
$
54.28
$
54.28
$
48.83
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 97
MEDIATECH (FISHER)
........................................................................................................ MED
_ Technical Services: (800) CELLGRO
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: No
_ Delivery: 3rd Business Day
PC CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
CH 1290
MT46-010-CM 10X TAE
1L
$
CH 1291
MT46011CM 10X TBE
1L
$
CH 1292
MT46020CM 20X SSC Buffer
1L
$
TC
1273
MT10013CV DMEM - high glucose 4.5 gm/L
500 ml
$
TC
1274
MT10017CV DMEM - high glucose 4.5 gm/L - no sodium pyruvate
500 ml
$
TC
1239
MT10013CM DMEM WITH L-GLUT; HI GLUCOSE; W/ NA PYRUVATE 1 L
$
TC
1260
MT10017CM DMEM WITH L-GLUT; HI GLUCOSE; W/O NA PYRUVATE 1 L
$
TC
1241
MT21030CM DPBS (1X) with Ca++ & Mg++
1L
$
TC
1243
MT21031CM DPBS (1X) without Ca++ & Mg++
1L
$
TC
1264
MT61234RF
G418
1g
$
TC
1265
MT61234RG G418
5g
$
TC
1282
MT30-234-CR G418 Sulfate (50mg/ml)
20 ml
$
TC
1245
MT10080CV Ham's F-12
500 ml
$
TC
1275
MT21020CM HBSS w/ ca and mg
1L
$
TC
1276
MT21021CM HBSS w/o ca and mg
1L
$
TC
1283
MT30-240-CR Hygromycin B (50mg/ml)
20 ml
$
TC
1247
MT10016CV Iscove's Medium
500 ml
$
CH 1294
MT46-050-CM LB BROTH
1L
$
TC
1249
MT25005CI
L-Glutamine - 200mM
100 ml
$
TC
1251
MT10010CM MEM - w/ Earles' salts & L-Glut
1L
$
TC
1253
MT30002CI
Pen/Strep - 100X -10mcg/ml P - 10mg/ml S
100 ml
$
TC
1255
MT10040CM RPMI 1640
1L
$
TC
1256
MT10041CV RPMI 1640 - w/ 25mM Hepes
500 ml
$
TC
1295
MT25-000-CI Sodium Pyruvate, 100mM solution
100 ml
$
CH 1293
MT25-900-CI Trypan Blue Solution
100ML
$
TC
1259
MT25052CI
Trypsin-EDTA - 0.05% T - .53mM EDTA
100 ml
$
MILLIPORE.......................................................................................MIL
_ Technical Services: (800) 225-1380
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 2nd Business Day
PC
CODE MFGR. NO.
DESCRIPTION
FM 2439
INYC13750
Immobilon-NY+ 137mm DISC(Colony Plaque)
FM 2214
IPVH20200
Immobilon™ P 20x20cm (Western Blot)
FM 2215
IPVH00010
Immobilon™ P 26.5cmx3.75m (Western Blot)
FM 2217
HATF08250
Nitrocellulose 82mm Colony & Plaque
FM 2216
HATF13750
Nitrocellulose 137mm Colony & Plaque
FM 2271
SCGPU02RE Stericup Filtr. & Stor.-PES 250ml .22µ
FM 2272
SCGPU05RE Stericup Filtr. & Stor.-PES 500ml .22µ
FM 2273
SCGPU11RE Stericup Filtr. & Stor.-PES 1000ml .22µ
FM 2433
SCGPU01RE Stericup Filtr. & Stor.-PES 150ml .22µ
FM 2339
SCGP00525
Steriflip-GP Filter Unit 50µl
FM 2434
SCGPS10RE
Steritop-GP Filtr 1000 ML 33 mm neck 0.22µm
FM 2435
SCGPT10RE Steritop-GP Filtr 1000 ML 45 mm neck 0.22µm
FM 2336
SCGPS05RE
Steritop-GP Filtr 500 ML 33 mm neck 0.22µm
FM 2335
SCGPT05RE Steritop-GP Filtr 500 ML 45 mm neck 0.22µm
SIZE
50/pk
10/pk
1 roll
50/pk
50/pk
12/pk
12/pk
12/pk
12/pk
25/pk
12/pk
12/pk
12/pk
12/pk
CC PRICE
$
156.48
$
101.81
$
125.90
$
49.63
$
101.81
$
42.64
$
66.82
$
95.45
$
33.09
$
80.25
$
65.54
$
65.54
$
41.36
$
41.36
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 98
12.70
11.65
9.51
3.98
3.98
7.96
7.96
7.96
7.96
58.11
201.69
63.10
4.24
7.96
7.96
97.69
6.37
13.78
4.77
7.96
4.77
7.96
5.84
4.30
6.63
4.78
FM
FM
FM
FM
2266
2264
2267
2265
SLGV025LS
SLHVO25LS
SLGS0250S
SLHA0250S
Syringe fltr-Durapore PVDF/PVC .22µ
Syringe fltr-Durapore PVDF/PVC .45µ
Syringe fltr-mixed esters of cellulose .22µ
Syringe fltr-mixed esters of cellulose .45µ
50/pk
50/pk
50/pk
50/pk
$
$
$
$
70.63
66.49
64.27
62.36
SIZE
50/pk
50/pk
10/pk
50/pk
1 roll
1 roll
50/pk
1 roll
CC PRICE
$
107.32
$
125.41
$
23.91
$
104.99
$
99.17
$
146.88
$
50.75
$
140.31
MICRON SEPARATIONS INC. (FISHER)
.............................................................................. MSI
_ Technical Services: (800) 444-8212
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 3rd Business Day
PC
CODE MFGR. NO.
DESCRIPTION
FM 2432
N00HN13750 Magna 137mm Notched
FM 2150
NJ0HY13750 Magnagraph 137mm
FM 2428
EP4HY408F0 Nitrobind 7x8.4
FM 2157
EP4HY13750 Nitrobind Pure 137mm
FM 2158
EP4HYA0010 Nitrobind Pure 20cmx3m
FM 2159
EP4HY00010 Nitrobind Pure 30cmx3m
FM 2164
WP4HY08250 Nitropure Supp 82mm
FM 2163
WP4HY00010 Nitropure Supp 30cmx3m
NATIONAL DIAGNOSTICS.................................................................ND
_ Technical Services: (800) 526-3867
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 1 - 2 Weeks
PC CODE MFGR. NO. DESCRIPTION
SIZE
CC PRICE
CH 2101
EC850
AccuGel™ 19:1
450 ml
$
CH 2177
EC890
ProtoGel® (30% Acrylamide/0.8% Bis)
1L
$
CH 2341
EC-892
ProtoGel® Buffer
450 ml
$
CH 2342
EC-893
ProtoGel® Stacking Buffer
200 ml
$
CH 2179
EC835
SequaGel® Buffer
200 ml
$
CH 2180
EC830
SequaGel® Concentrate (20% Acryl.) 19:1
1L
$
CH 2181
EC840
SequaGel® Diluent
1L
$
CH 2182
EC833
SequaGel® Sequencing System
1 kit
$
CH 2184
EC836
SequaGel®-6 6% Monomer Sequencing Soln (includes EC-841) 1 L
$
CH 2183
EC838
SequaGel®-8 8% Monomer Sequencing Soln (includes EC-841) 1 L
$
NEW ENGLAND BIOLABS
...................................................................................................NEB
_ Technical Services: (800) 632-5227
_ Website: http://www.neb.com/
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: All items in the New England Biolabs catalog may be special ordered, no shipping or dry ice
fees.
_ Conditions: All special orders for NEB products must be received at the Cell Center by 2pm for next day availability.
_ Items ordered on Friday will be available on Monday.
_ Delivery: Overnight
PC CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
MR 4584
N3232S
1 kb DNA Ladder
100ug
$
40.44
MR 4583
N3231S
100 bp DNA Ladder
50ug
$
40.44
RE 6101
R0117S
Aat II
500u
$
36.77
RE 6104
R0161S
Acc I
500u
$
44.12
RE 6107
R0520S
Afl II
2000u
$
36.77
RE 6312
R0541S
Afl III
250u
$
40.44
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 99
43.76
72.98
19.36
16.00
10.00
54.20
20.90
85.58
37.44
39.98
OE
RE
RE
MB
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
OE
RE
RE
OE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
OE
RE
MR
MR
MB
RE
RE
RE
RE
RE
RE
RE
RE
RE
5307
6410
6414
4330
6111
6288
6323
6116
6385
6416
6119
6297
6124
6128
6298
6408
6299
6387
6381
6137
6384
6388
5110
6139
6142
5112
6140
6389
6390
6143
6300
6147
6301
6152
6159
6325
6166
6303
6176
6180
6182
5122
6185
4252
4137
4331
6190
6324
6123
6413
6193
6305
6411
6201
6204
M0392S
R0552S
R0514S
E8021L
R0114S
R0558S
R0526S
R0153S
R0174S
R0174L
R0136S
R0136L
R0136T
R0160S
R0144S
R0144M
R0540S
R0517S
R0199S
R0162S
R0113S
R0524S
M0290S
R0197S
R0175S
M0209S
R0176S
R0129S
R0510S
R0505S
R0521S
R0101S
R0101L
R0101T
R0107S
R0139S
R0103S
R0104S
R0155S
R0105S
R0171S
M0210S
R0142S
N3011S
N3012S
S1404S
R0147S
R0589S
R0198S
R0163S
R0534S
R0190S
R0191S
R0193S
R0111S
Agarase
Age I
AlwN I
Amylose Resin
Apa I
Asc I
Ase I
Ava II
Avr II
Avr II
BamH I
BamH I
BamH I HC
Bcl I
Bgl II
Bgl II
BspE I
BspH I
BssH II
BstE II
BstX I
Bsu36 I
Calf Intestinal Alkaline Phosphatase
Cla I
Dde I
DNA Polymerase I from E.coli
Dpn I
Dra I
Dra III
Eag I (Xma III)
EcoN I
EcoR I
EcoR I
EcoR I HC
Hae II
Hha I
Hinc II
Hind III
Hinf I
Hpa I
Hpa II
Klenow - Sequencing Grade
Kpn I
Lambda DNA
Lambda DNA/HindIII Fragment
m7GpppG 5'G RNA Cap Structure
Mbo I
Mfe I
Mlu I
Mnl I
Msc I
Nae I
Nar I
Nco I
Nde I
100u
250u
500u
100 ml
2000u
500u
2000u
2000u
100u
500u
10000u
50000u
10000u
3000u
2000u
10000u
1000u
500u
200u
2000u
1000u
500u
1000u
1000u
500u
500u
1,000u
2000u
1000u
500u
1000u
10000u
50000u
10000u
2000u
2000u
1000u
10000u
5000u
500u
2000u
200u
3000u
250 µg
150 µg
25u
500u
500u
1000u
250u
100u
500u
200u
1000u
4000u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
44.12
40.44
40.44
220.61
44.12
40.45
40.45
36.77
44.12
176.48
36.77
147.07
36.77
36.77
36.77
147.07
40.44
40.44
40.44
36.77
40.44
40.44
40.44
40.44
36.77
40.44
40.44
40.44
40.44
40.44
40.44
36.77
147.07
36.77
36.77
36.77
36.77
36.77
36.77
36.77
36.77
36.77
36.77
40.44
40.44
110.31
44.12
44.13
40.36
40.44
40.44
36.77
36.77
36.77
40.44
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 100
RE
RE
RE
RE
RE
RE
RE
MB
MR
MR
RE
MR
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
OE
OE
OE
OE
OE
OE
PC
PC
RE
RE
RE
RE
RE
RE
6205
6308
6210
6309
6213
6214
6409
4254
4250
4251
6412
4546
6220
6227
6230
6234
6314
6236
6315
6322
6241
6246
6249
6252
6254
6258
6392
6263
6383
5165
5217
5166
5169
5173
5218
5257
5195
6271
6320
6274
6287
6393
6280
R0131S
R0131L
R0189S
R0189L
R0192S
R0127S
R0547S
N3032S
N3026L
N3026S
R0560S
P7708S
R0140S
R0151S
R0167S
R0156S
R0157S
R0138S
R0138L
R0569S
R0169S
R0123S
R0141S
R0130S
R0133L
R0182S
R0132S
R0187S
R0500S
M0202S
M0202L
M0202T
M0203S
M0201S
M0201L
M0257S
M0254S
R0145S
R0145L
R0146S
R0146L
R0180S
R0194S
Nhe I
Nhe I
Not I
Not I
Nru I
Nsi I
Pac I
pBR322/Msp I Digest
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment
Pme I
Prestained Protein Molecular Weight Marker
Pst I
Pvu II
Rsa I
Sac I (Sst I)
Sac II
Sal I
Sal I
Sap I
Sau 3A I
Sfi I
Sma I
SnaB I
Spe I
Sph I
Ssp I
Stu I
Sty I
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase HC
T4 DNA Polymerase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
Vent® (exo-) Polymerase
Vent® DNA Polymerase
Xba I
Xba I
Xho I
Xho I
Xma I
Xmn I
1000u
5000u
500u
2500u
1000u
1000u
250u
50 µg
250u
50u
500u
175 lns
10000u
5000u
1000u
2000u
2000u
2000u
10000u
50u
200u
2000u
2000u
500u
2500u
250u
1000u
1000u
3000u
20000u
100000u
20000u
150u
500u
2500u
200u
200u
3000u
15000u
5000u
25000u
250u
1000u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
40.44
161.78
44.12
176.48
36.77
40.44
40.44
40.44
161.78
40.44
44.12
62.51
40.44
36.77
36.77
36.77
36.77
36.77
147.07
40.45
36.77
40.44
36.77
36.77
161.78
40.44
40.44
36.77
44.12
44.12
176.48
44.12
40.44
36.77
147.07
58.83
58.83
44.12
176.48
44.12
176.00
40.44
36.77
PERKIN ELMER (NEW ENGLAND NUCLEAR)
................................................................... NEN
_ Technical Services: (800) 632-5227
_ Web Site: www.perkinelmer.com
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: Many non-radioactive items in the NEN catalog may be special ordered, no shipping or dry
ice fees.
_ Items ordered on Friday will be available on Tuesday.
_ Delivery: 2nd Day
PC CODE MFGR. NO. DESCRIPTION
SIZE
CC PRICE
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 101
PC
PC
NR
FM
FM
FM
FM
FM
NR
NR
NR
NR
FM
FM
5455
5456
5979
2260
2250
2246
2249
2253
5201
3120
3121
3122
2388
2385
NEL542
NEL999
NEL-601
NEF-978Y
NEF-986
NEF-976
NEF-984
NEF-1002
NEL-201
NEL-103
NEL-104
NEL-105
NEF-586
NEF-596
Biotin 11 CTP
250nm
Biotin 16 UTP
250nm
CDP-Star Nucleic Acid Chemiluminescence Reagent (Ready to Use) 1 kit
Colony/Plaque Screen™ w/orient. holes 137mm
50/bx
Genescreen Plus® Hybridization Mem. 20x20cm
10/bx
Genescreen Plus® Hybridization Mem. 30x45cm
5 sht
Genescreen™ Hybridization Mem. 20x20cm
10/bx
Polyscreen® PVDF 30cmx3m
1 roll
Renaissance™ Nucleic Acid Chemilum Rgt Kit
1 kit
Renaissance™ Western Blot Chemilum Rgt
1000cm2
Renaissance™ Western Blot Chemilum Rgt
2500cm2
Renaissance™ Western Blot Chemilum Rgt
5000cm2
X-Omat™ Blue 5x7
100/bx
X-Omat™ Blue 8x10in
100/bx
$
$
$
$
$
$
$
$
$
$
$
$
$
$
APPLIED BIOSYSTEMS
....................................................................................................... PEC
_ Technical Services: (800) 762-4001
_ Web Site: http://www.appliedbiosystems.com/
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: All items in the PEC catalog may be special ordered at a discount, no shipping or dry ice fees.
_ Conditions: All special orders for PEC products must be received at the Cell Center by 2pm for next day availability.
_ Items ordered on Friday will be available on Monday.
_ Delivery: Overnight
PC
CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
PC
5993
N808-0246
AmpliTaq Gold™ w/ buffer I - 1000u
1000u
$
284.65
PC
5382
N808-0240
AmpliTaq Gold™ w/ buffer I - 250u
250u
$
86.08
PC
5997
N808-0244
AmpliTaq Gold™ w/ buffer I-12 pac (250u)
3000u
$ 1,032.97
PC
5999
N808-0248
AmpliTaq Gold™ w/ buffer I-5 pac (1000u)
5000u
$ 1,413.18
PC
5995
N808-0242
AmpliTaq Gold™ w/ buffer I-6 pac (250u)
1500u
$
516.49
PC
5994
N808-0247
AmpliTaq Gold™ w/ buffer II - 1000u
1000u
$
284.65
PC
5383
N808-0241
AmpliTaq Gold™ w/ buffer II - 250u
250u
$
86.08
PC
5998
N808-0245
AmpliTaq Gold™ w/ buffer II-12 pac (250u)
3000u
$ 1,032.97
PC
6000
N808-0249
AmpliTaq Gold™ w/ buffer II-5 pac (1000u)
5000u
$ 1,413.18
PC
5996
N808-0243
AmpliTaq Gold™ w/ buffer II-6 pac (250u)
1500u
$
516.49
PC
5263
N808-0171
AmpliTaq® w/ buffer I - 1000u
1000u
$
288.37
PC
5264
N808-0152
AmpliTaq® w/ buffer I - 12 paq (250u)
3000u
$
900.62
PC
5212
N808-0160
AmpliTaq® w/ buffer I - 250u
250u
$
75.05
PC
5265
N808-0155
AmpliTaq® w/ buffer I - 5 paq (1000u)
5000u
$ 1,431.72
PC
5266
N808-0166
AmpliTaq® w/ buffer I - 6 paq (250u)
1500u
$
450.32
PC
5267
N808-0172
AmpliTaq® w/ buffer II - 1000u
1000u
$
288.37
PC
5213
N808-0161
AmpliTaq® w/ buffer II - 250u
250u
$
75.05
PC
5268
N808-0156
AmpliTaq® w/ buffer II - 5 paq (1000u)
5000u
$ 1,431.72
PC
5269
N808-0167
AmpliTaq® w/ buffer II - 6 paq (250u)
1500u
$
450.32
PC
5270
N808-0153
AmpliTaq® w/ buffer II- 12 paq (250u)
3000u
$
900.62
PC
5251
N808-0150
AmpliWax® PCR Gem 50
200 gem
$
73.50
PC
5250
N808-0100
AmpliWax® PCR Gem 100
200 gem
$
73.50
PC
5407
4303573
BigDye™ Terminator Kit
24 rxn
$
192.37
PC
5404
N808-0260
GeneAmp® dNTP blend 10mM
1 ml
$
29.40
PC
5406
4303442
GeneAmp® dNTP blend 10mM-12 paq
12 ml
$
352.80
PC
5405
4303441
GeneAmp® dNTP blend 10mM-6 paq
6 ml
$
176.41
PC
5191
N808-0009
GeneAmp® Core Kit
100 rxn
$
164.21
PC
5247
N808-0007
GeneAmp® dNTP Set -10mM (4x3.2umol)
1 set
$
36.50
PC
5253
N808-0179
GeneAmp® EZ rTth RNA PCR Kit
100 rxn
$
355.64
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 102
187.07
186.03
134.25
231.21
150.24
218.43
149.17
181.14
131.06
77.79
140.65
198.18
57.53
135.27
PC
PC
PC
PC
PC
PC
PC
PC
PC
5202
5248
5255
5254
5249
4628
4629
4626
4627
N808-0143
N808-0193
N808-0178
N808-0098
N808-0180
4309155
4312704
4304437
4305719
GeneAmp® RNA PCR Core Kit
GeneAmp® XL PCR Kit
rTth DNA Polymerase & EZ Buffer Pack
rTth DNA Polymerase with Buffer
rTth DNA Polymerase-XL w/XL Buffer Pack
Sybr Green PCR Master Mix
Sybr Green PCR Master Mix
TaqMan Universal Master Mix
TaqMan Universal Master Mix
100 rxn
100 rxn
100 rxn
100 rxn
100 rxn
pk
10 pk
200 rxn
2000 rxn
$
$
$
$
$
$
$
$
$
237.21
375.40
229.19
229.19
190.34
273.52
2,679.00
310.00
3,035.00
POLAROID
...............................................................................................................POL
_All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: NO
_ Delivery: 3rd Business Day
PC
CODE MFGR. NO.
DESCRIPTION
FM 2322
444159
Polaroid Film Type 57
FM 2323
444191
Polaroid Film Type 667
SIZE
pk
pk
CC PRICE
$
47.95
$
24.07
PROMEGA...........................................................................................PRO
_ Technical Services: (800) 356-9526
_ Web Site: http://www.promega.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: No
_ Delivery: Overnight
PC
CODE MFGR. NO.
DESCRIPTION
MR 4588
PRG6941
1 kb DNA Step Ladder
MR 4585
G4471
10 bp DNA Step Ladder
MR 4455
G2101
100 bp DNA Ladder
MR 4586
G6951
100 bp DNA Step Ladder
MR 4587
G6961
200 bp DNA Step Ladder
MR 4590
G4521
50 bp DNA Ladder
OE 5105
M5108
AMV Reverse Transcriptase
OE 5106
M5101
AMV Reverse Transcriptase
RE 6396
R6361
Apa I
RE 6121
R6021
BamH I
RE 6398
R6071
Bgl I
RE 6133
R6081
Bgl II
RE 6400
R6641
BstE II
OE 5256
M1821
Calf Intestinal Alkaline Phosphatase
RE 6289
R6551
Cla I
PC
4241
U1201
dATP (100mM)
PC
4242
U1221
dCTP (100mM)
RE 6401
R6291
Dde I
PC
4243
U1211
dGTP (100mM)
OE 5204
M2051
DNA Polymerase I
OE 5144
M6101
DNase RQ1 RNase-free
PC
4240
U1240
dNTP's (bulk) (100mM) 4x40umoles
PC
4244
U1231
dTTP (100mM)
RE 6149
R6011
EcoR I
RE 6290
R6351
EcoR V
OE 5262
M1811
Exonuclease III
MB 4248
Q4100
fmol® DNA Sequencing System
CE 4246
L2011
HB101 High Efficiency Competent Cells
SIZE
300 lns
50 µl
250 µl
100 µl
100 µl
100 lns
1000u
300u
5000u
2500u
1000u
500u
2000u
1000u
500u
40 µmol
40 µmol
200u
40 µmol
500u
1000u
1 set
40 µmol
5000u
2000u
5000u
1 kit
1 ml
CC PRICE
$
58.57
$
57.08
$
56.38
$
75.92
$
78.51
$
65.48
$
131.21
$
50.41
$
38.32
$
24.47
$
30.95
$
22.24
$
38.32
$
35.58
$
35.58
$
61.74
$
61.74
$
17.81
$
61.74
$
66.72
$
22.19
$
193.18
$
61.74
$
17.80
$
31.14
$
45.70
$
143.73
$
58.83
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 103
RE
RE
RE
CE
OE
OE
RE
MR
NR
NR
NR
OE
RE
RE
RE
RE
RE
RE
RE
MB
PC
MB
MB
MB
MR
CO
RE
RE
RE
MB
OE
MB
MB
MB
MB
OE
RE
RE
RE
RE
RE
RE
RE
OE
RE
RE
RE
RE
RE
OE
OE
OE
OE
OE
PC
6358
6172
6361
4247
5366
5123
6363
4486
3185
3212
3211
5367
6364
6365
6203
6367
6206
6292
6368
4268
4199
5942
5943
4294
4198
4528
6369
6370
6371
4169
5205
4176
4177
4174
4175
5207
6373
6374
6238
6375
6243
6251
6376
5158
6293
6260
6403
6377
6404
5161
5208
5209
5175
5179
5982
R6441
R6041
R6301
L2001
M2181
M2201
R6341
G1711
E4030
E1501
E4530
M1701
R6711
R6381
R6513
R6801
R6501
R6431
R7091
C1101
PRG3161
A1360
A1380
A3600
G1761
Z5400
R6111
R6321
R6331
L4960
M4281
N2515
N2511
N2115
N2111
M5761
R6061
R6221
R6051
R6191
R6211
R6121
R6791
P1085
R6591
R6261
R6601
R6421
R6481
P2083
M1801
M4211
M4101
P2075
M1868
Hha I
Hind III
Hpa I
JM109 High Efficiency Competent Cells
Klenow - Exo Minus
Klenow Fragment
Kpn I
Lambda DNA/HindIII Fragment
Luciferase Assay System
Luciferase Assay System (10 pk) quote#5075
Luciferase Assay System–10 pack
Maloney-MLV Reverse Transcripts
Mbo I
Mlu I
Nco I
Nde I
Nhe I
Not I
Nru I
Oligo(dT) 15 primer
PCR Markers
pGEM®-T Easy I
pGEM®-T Easy II
pGEM®-T Vector I
Phi-X174 DNA/Hae III Fragment
PolyATtract® System 1000 RNA Isolation System
Pst I
Pvu I
Pvu II
Rabbit Reticulocyte Lysate
Ribonuclease H
RNasin® Recombinant Ribonuclease Inhibitor
RNasin® Recombinant Ribonuclease Inhibitor
RNasin® Ribonuclease Inhibitor
RNasin® Ribonuclease Inhibitor
S1 Nuclease
Sac I (Sst I)
Sac II
Sal I
Sau 3A I
Sca I
Sma I
SnaB I
Sp6 RNA Polymerase
Spe I
Sph I
Ssp I
Stu I
Sty I
T3 RNA Polymerase
T4 DNA Ligase
T4 DNA Polymerase
T4 Polynucleotide Kinase
T7 RNA Polymerase
Taq DNA Polymerase
1000u
5000u
100u
1 ml
100u
150u
2500u
100µg
100 assays
1000 assays
1000 assays
10000u
200u
1000u
200u
500u
250u
200u
200u
20 µg
250 µl
1 kit
1 kit
20u
50 µg
1 kit
3000u
100u
1000u
1 ml
50u
10000u
2500u
10000u
2500u
10000u
1000u
500u
2000u
100u
1000u
1000u
100u
1000u
200u
200u
500u
400u
2000u
1000u
100u
100u
100u
1000u
2500u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
26.53
14.09
29.48
67.61
27.43
54.87
33.17
24.45
46.84
308.22
412.41
31.88
36.12
35.58
48.92
66.34
40.77
35.58
34.65
33.28
54.12
89.93
151.91
74.41
65.24
279.25
18.53
49.70
22.85
94.41
97.86
173.32
61.49
163.43
64.86
19.27
29.48
37.07
38.55
24.33
35.58
39.07
29.48
94.73
45.21
44.94
49.39
29.48
35.38
22.97
22.98
48.19
27.43
30.39
433.71
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 104
PC
PC
OE
MB
KT
KT
KT
KT
KT
KT
IM
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
RE
RE
5189
5258
5210
5944
5930
5931
5929
5928
5933
5932
3203
4142
4143
4144
4145
4195
4292
4293
4147
4146
4291
4529
4531
6379
6380
M1861
M1865
M1871
E2381
L4600
L5020
L4950
L4610
L1170
L5010
S3841
PRA7280
PRA7290
PRA7270
A7300
PRA7640
A7211
PRA7141
A7170
A7100
A7510
A1330
A1460
R6181
R6161
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Terminal Deoxy Transferase (TdT)
Tfx-10 Transfection Reagent
TNT®-SP6 Coupled Transcription/Translation
TNT®-SP6/T7 Coupled Transcription/Translation
TNT®-T3 Coupled Transcription/Translation
TNT®-T7 Coupled Transcription/Translation
TNT®-T7 QUICK Coupled Transcription/Translation
TNT®-T7/T3 Coupled Transcription/Translation
Western Blue Stabilized Substrate for AP
Wizard™ DNA Clean-up System
Wizard™ Lambda System
Wizard™ Maxipreps System
Wizard™ Megapreps System
Wizard™ Midipreps System
Wizard™ Minicolumns
Wizard™ Miniprep DNA Purification Resin
Wizard™ PCR Preps System
Wizard™ Plus Miniprep System
Wizard™ Plus Miniprep System
Wizard™ Plus SV
Wizard™ Plus SV
Xba I
Xho I
100u
500u
300u
1 ea
1 kit
1 kit
1 kit
1 kit
1 kit
1 kit
1 ea
100 col
20 col
10 col
5 col
25 col
250 col
250 ml
50 col
50 col
250 col
50 rxn
250 rxn
2000u
3000u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
24.33
82.75
39.29
143.21
291.15
300.09
312.11
293.37
297.05
314.95
30.78
83.87
56.77
90.35
86.36
111.78
99.95
94.35
59.20
65.09
222.20
42.56
189.73
38.55
24.46
QIAGEN
...........................................................................................................QIA
_ Technical Services: (800) 426-8157
_ Web Site: http://www.qiagen.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: All items in the QIA catalog may be special ordered, no shipping or dry ice fees.
_ Conditions: All special orders for QIA products must be received at the Cell Center by 2pm for next day availability.
_ Items ordered on Friday will be available on Monday.
_ Delivery: Overnight
PC CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
CO 4603
34698
Anti-His Antibody Selector Kit (3 x 3 µg)
3x3ug
$
70.88
KT 5473
69504
DNeasy Tissue Kit
50 $
98.69
CO 4601
301425
Effectene Transfection Reagent (1 ml)
1 ml
$
197.38
CO 4502
12362
EndoFree Plasmid Maxi Kit (10)
kit
$
211.73
CO 4602
12643
Hispeed Midi Kit (25) 45 minutes/200ug
45min/200ug $
220.71
KT 5471
12662
Hispeed Plasmid Maxi Kit
10 prep
$
183.02
KT 5472
12663
Hispeed Plasmid Maxi Kit
25 prep
$
442.31
PC
6100
203205
Hot Star Taq (1000) with Q solution
(4x250u)
$
261.24
PC
6099
203203
Hot Star Taq (250) with Q solution
250u
$
92.42
CO 4503
30410
Ni-NTA Superflow
25 ml
$
221.60
MS 3345
2003482
Oligo CD - $1,000
EA
$ 1,000.00
MS 3344
2003481
Oligo CD - $500
EA
$
500.00
CO 4318
51104
QIAamp™ Blood Kit
50 prp
$
90.61
CO 4319
51304
QIAamp™ DNA Mini Kit
50 prp
$
100.48
CO 4316
30210
QIAexpress™ Ni-NTA Agarose Kit
25 ml
$
176.74
CO 4158
20021
QIAEX™ II Gel Extraction Kit
150 prp
$
101.38
CO 4564
12191
QIAGEN Plasmid Giga Kit
5 prp
$
324.78
CO 4159
12162
QIAGEN Plasmid Maxi Kit
10 col
$
151.62
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 105
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
PC
CO
CO
CO
PC
PC
4317
4565
4281
4161
4163
4566
4203
4567
4568
4204
4504
4255
4282
4634
4595
4256
4547
6001
6025
12163
12165
12181
12143
12123
27106
27104
28144
28706
28704
28304
28104
79654
204143
74106
74104
301305
201203
201207
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Mega Kit
QIAGEN Plasmid Midi Kit
QIAGEN Plasmid Mini Kit
QIAprep Spin Miniprep Kit
QIAprep™ Spin Miniprep Kit
QIAquick 8 PCR Purification Kit
QIAquick Gel Extraction Kit
QIAquick™ Gel Extraction Kit
QIAquick™ Nucleotide Removal Kit (50)
QIAquick™ PCR Purification Kit
QIAshredder™
QuantiTect SYBR Green PCR Kit
RNeasy Mini Kit
RNeasy™ Mini RNA Kit
SuperFect Transfection Reagent (1.2 ml)
Taq DNA Polymerase
Taq DNA Polymerase
25 col
100 prp
5 col
25 col
25 col
250 prp
50 rxn
400 prp
250 prp
50 prp
kit
50 rxn
50 cap
200 rxn
250 prp
50 prp
3 mg/ml
250u
10x250u
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
359.77
1,327.82
187.51
177.64
122.02
253.00
58.32
538.31
329.26
70.88
70.88
70.88
48.45
296.07
768.88
174.95
171.36
50.37
1,044.95
BIO–RAD
...........................................................................................................RAD
_ Technical Services: (800) 424-6723
_ Web Site: http://www.bio-rad.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: All items in the RAD catalog may be special ordered, no shipping or dry ice fees.
_ Conditions: All special orders for RAD products must be received at the Cell Center by 2pm for 2nd day availability.
_ Items ordered on Friday will be available on Tuesday
_ Delivery: 2nd Day
PC
CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
CH 2392
1610154
30% Acrylamide/Bis 19:1
500 ml
$
39.32
CH 2393
1610156
30% Acrylamide/Bis 29:1
500 ml
$
39.32
CH 2394
1610158
30% Acrylamide/Bis 37.5:1
500 ml
$
39.32
CH 2389
1610144
40% Acrylamide/Bis 19:1
500 ml
$
45.57
CH 2390
1610146
40% Acrylamide/Bis 29:1
500 ml
$
45.57
CH 2391
1610148
40% Acrylamide/Bis 37.5:1
500 ml
$
45.57
CH 2347
1610101
Acrylamide
500 g
$
99.19
CH 2352
1610700
Ammonium Persulfate
10 g
$
10.72
CH 6058
1610786
Bio-Safe Coomasie Stain
1L
$
51.41
CH 2350
1610201
Bis-Acrylamide
50 g
$
28.60
CH 5908
1610710
BME (2-mercaptoethanol)
25ml
$
16.98
CH 5911
1610404
Bromophenol Blue
10g
$
27.70
CH 5909
1610400
Coomassie™ Brilliant Blue R-250
10g
$
16.08
KT 5941
5000112
DC Protein Assay Kit II
1 kit
$
108.13
CH 6060
1610611
Dithiothreitol (DTT)
5g
$
52.48
MS 5950
1652089
Gene Pulser® Cuvettes 0.1cm
50 pk
$
116.69
MS 4517
1652086
Gene Pulser® Cuvettes 0.2cm
50 pk
$
124.21
MS 4518
1652088
Gene Pulser® Cuvettes 0.4cm
50 pk
$
116.69
CH 2354
1610718
Glycine
1 kg
$
29.49
FM 2440
1620174
Immun-Blot PVDF
10 sht
$
55.49
MR 4515
1610324
Kaleidoscope Prestained Standards
500 µl
$
89.36
FM 2372
1620147
Nitrocellulose Membrane (.20u) 13.5x16.5cm
10 sht
$
126.00
FM 2370
1620146
Nitrocellulose Membrane (.20u) 7x8.4cm
10 sht
$
46.47
FM 2373
1620115
Nitrocellulose Membrane (.45u) 33cmx3m
1 roll
$
171.57
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 106
CH
MR
MR
MR
MR
KT
KT
CO
CO
CO
PG
PG
PG
PG
FM
FM
KT
CH
CH
CH
CH
CH
CH
FM
2395
4633
4483
4482
4481
5937
5939
5437
5438
4522
3340
3342
3341
3343
2371
2368
5360
5910
2355
2351
2353
2397
6059
2362
1610202
161-0374
1610318
1610309
1610305
5000006
5000002
7326130
7326120
7326100
1611101
1611155
1611104
1611158
1620181
1620186
1610449
1610301
1610733
1610800
1610719
1610732
1706531
1620192
PDA
Precision Plus Protein Dual Color Standard
Prestained Standards Broad
Prestained Standards High
Prestained Standards Low
Protein Assay Kit Dye Reagent Concentrate
Protein Assay Kit II
Quantum Maxi-Prep Kit
Quantum Midi-Prep Ket
Quantum™ Prep Plasmid Miniprep
ReadyGel, 10% Tris-Hcl, 10 well
ReadyGel, 10% Tris-Hcl, 50ul
ReadyGel, 4-15% Tris-Hcl, 10 well
ReadyGel, 4-15% Tris-Hcl, 50ul
Sequi-Blot™ PVDF Membrane (.20u) 15x15cm
Sequi-Blot™ PVDF Membrane (.20u) 7x8.4cm
Silver Stain Plus Kit
Sodium Dodecyl Sulfate (SDS)
TBE 10X
TEMED
Tris
TRIS/Glycine/SDS 10X Buffer
Tween 20
Zeta-Probe® GT Membrane15x15cm
10 g
50 app
500 µl
500 µl
500 µl
1 kit
1 kit
10 prp
20 prp
kit
EA
EA
EA
EA
10 sht
10 sht
kit
100g
1L
5 ml
1 kg
1L
100 ml
15 sht
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
75.96
93.76
80.42
75.96
68.81
69.70
81.32
107.23
107.23
107.23
8.14
8.14
8.14
8.14
99.19
64.34
116.69
25.91
21.02
13.40
55.40
19.66
35.81
180.51
RAININ
................................................................................................................RAI
_ Technical Services: (800) 828-2788
_ All items listed below are available on site at the Cell Center.
_ SPECIAL ORDERING: Some items in the Rainin catalog may be special ordered. Orders must be received in the
Cell Center before 2pm for 3rd day delivery.
_ Delivery: 3rd Business Day
PC
CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
MS 5955
P-2
Pipetman 0.1-2.0µl
1 ea
$
266.92
MS 5956
P-10
Pipetman 0.5-10µl
1 ea
$
266.92
MS 5954
P-1000
Pipetman 100-1000µl
1 ea
$
234.45
MS 5952
P-100
Pipetman 10-100µl
1 ea
$
234.45
MS 5951
P-20
Pipetman 2-20µl
1 ea
$
234.45
MS 5953
P-200
Pipetman 50-200µl
1 ea
$
234.45
SIGMA-ALDRICH
............................................................................................................... SIG
_ Technical Services: (800) 521-8956
_ Web Site: http://www.sigma-aldrich.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: All items in the Sigma catalog may be special ordered, no shipping or dry ice fees.
_ Conditions: All special orders for Sigma products must be received at the Cell Center by 2pm for 2nd day
availability.
_ Items ordered on Friday will be available on Wednesday
_ Delivery: 2nd Business Day
PC CODE MFGR. NO. DESCRIPTION
SIZE
CC PRICE
SM 7135
F2442-500
Fetal Bovine Serum, lot #102K8410, 110% efficiency
500 ml
$
CH 5913
A8456-100mg 4-(2-Aminoethyl)Benzenesulfonyl*Fluoride Hydrochloride
100 mg
$
CH 5912
A6279-10ml
Aprotinin from Bovine Lung
10 ml
$
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 107
179.47
66.71
80.37
CH
PC
CH
CH
CH
CH
CH
KT
CO
MB
PC
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
PC
PC
PC
PC
PC
CH
CH
CH
CH
CH
CH
CH
CH
2446
5411
5914
6062
2445
2375
2376
4016
4539
6067
5410
5916
5917
6063
6066
5448
5918
2403
2404
2398
2401
2402
6065
2399
6055
5477
5408
5409
5414
5922
5923
6064
5921
5919
5920
1287
1288
B2518-100mg BOVINE SERUM ALBUMIN MOLECULAR BIOLOGY GRADE 100g
D7295-.5ml
Deoxynucleotide Mix
1 vl
D2650-5x5ml Dimethyl Sulfoxide Hybri-Max® Sterile*Filtered
25 ml
D2650-100ml DMSO
100 ml
D9891-25g
DOXYCYCLINE HYDROCHLORIDE
25g
E1510
Ethidium Bromide Aqueous Solution 10mg/ml
10 ml
E4391
Ethidium Bromide Tablets 10mg
10 ea
XNAT-100RXN Extract n Amp Red
100 rxn
May-00
Genelute Agarose Spin Column
70 ea
G4877-10000iu GONADOTROPIN FROM PREGNANT MARES' SERUM
10000iu
D5062-500u
Klentaq LA DNA Polymerase
500u
L7533
LB Agar - EZMix®
6 x 500 ml
L7658-1kg
LB Broth - EZMix®
1 kg
L2884-10mg
Leupeptin Hemisulfate from Microbial Source
10 mg
H7033-125ml Perfect Hyb Plus Hybridization Buffer
125 ml
H7033-1L
Perfect Hyb Plus Hybridization Buffer
1L
P7626-5g
Phenylmethylsulfonyl Fluoride
5g
P8465-5ml
Protease Inhibitor Cocktail-Bacterial
5 ml
P8465-25ml
Protease Inhibitor Cocktail-Bacterial
25 ml
P2714
Protease Inhibitor Cocktail-General
1 btl
P8340-1ml
Protease Inhibitor Cocktail-Mammalian w/o Metal Chelators
1 ml
P8340-5ml
Protease Inhibitor Cocktail-Mammalian w/o metal chelators
5 ml
P8849-5ml
Protease Inhibitor Cocktail-Poly His Tagged
5 ml
P8215-1ml
Protease Inhibitor Cocktail-Yeast/Fungal
1 ml
D4309-250u
Redtaq polymerase
250 unit
S4438-100rxn SYBR Green qPCR
100 rxn
D1806-250u
Taq DNA Polymerase
250u
D4545-250u
Taq DNA Polymerase w/10X Buffer
250u
T4809
Taqstart Antibody
200 tst
T9179
Terrific Broth (Modified)-EZMix®
6 x 500ml
T9424-25ml
Tri Reagent
25 ml
T9424-100ml Tri Reagent
100 ml
T6066-1Kg
TRIZMA® Base Molecular Biology Reagent Grade
1 kg
T1503-1kg
TRIZMA® Base Reagent Grade
1 kg
T3253-1kg
TRIZMA® Hydrochloride Reagent Grade
1 kg
T2444
Trizma-Hcl, 1 M Stock Solution, pH 7.6
1L
T2694
Trizma-Hcl, 1 M Stock Solution, pH 8.0
1L
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
STRATAGENE
..............................................................................................................STR
_ Technical Services: (800) 426-8157
_ Web Site: http://www.stratagene.com
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: All items in the STR catalog may be special ordered, no shipping or dry ice fees.
_ Conditions: All special orders for STR products must be received at the Cell Center by 2pm for next day availability.
_ Items ordered on Friday will be available on Tuesday
_ Delivery: Overnight
PC
CODE MFGR. NO.
DESCRIPTION
SIZE
CC PRICE
CE 5428
230245
BL21-Codon Plus (DE3)-RIL Competent Cells
ea
$
196.77
CE 5430
230255
BL21-Codon Plus (DE3)-RP Competent Cells
ea
$
196.77
CE 5427
230240
BL21-Codon Plus-RIL Competent Cells
ea
$
196.77
CE 5429
230250
BL21-Codon Plus-RP Competent Cells
ea
$
196.77
PC
6090
600262
Herculase Enhanced DNA Polymerase
500u
$
295.62
PC
6089
600260
Herculase Enhanced DNA Polymerase
100u
$
68.03
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 108
95.16
25.61
29.82
84.34
174.74
34.25
31.02
147.21
76.00
339.13
162.58
21.68
122.11
57.64
75.03
427.49
43.08
29.43
118.05
24.85
29.74
118.82
118.05
29.49
84.14
121.46
78.69
80.27
183.51
67.48
24.11
75.55
72.53
71.70
113.46
16.69
16.69
KT
PC
PC
PC
PC
PC
PC
KT
KT
MB
MB
CE
CE
CE
CE
CE
CE
CE
4260
5400
5401
4008
5402
5403
5447
5138
5934
4551
4552
4550
4129
5418
4258
4548
4130
5517
211190
600153
600154
600380
600250
600252
600320
300385
200518
201220
201190
200152
200238
200315
200228
200314
200236
200150
pCR-Script™ Amp Cloning Kit, (3 parts, -80,-20, RT)
Pfu DNA Polymerase
Pfu DNA Polymerase
Pfu Ultra High Fidelity
PfuTurbo™ DNA Polymerase
PfuTurbo™ DNA Polymerase
PfuTurbo™ HotStart DNA Polymerase
Prime-It® II Random Priming Kit
QuickChange™ Site-Directed Mutagenesis Kit
QuickHyb® Rapid Hybridization Buffer
Salmon Sperm DNA
SURE® 2 Supercompetent Cells
SURE® Competent Cells
XL 10-Gold Ultracompetent Cells
XL-1 Blue Electroporation Competent Cells
XL10-Gold Ultracompetent Cells
XL-1-Blue Supercompetent Cells
XL-2 Blue Ultra Competent Cells
1 kit
100u
500u
100 u
100u
500u
100u
30 rxn
1 kit
250 ml
10 x 1ml
ea
5x0.2ml
10 x 100µl
5x80µl
5x100ul
5x0.2ml
ea
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
$
263.71
85.98
345.80
102.99
93.54
382.65
104.88
155.58
475.24
154.95
156.16
156.21
152.30
194.93
161.25
129.39
146.57
183.82
USB CORPORATION........................................................................USB
_ Technical Services: (800) 321-9322
_ All items listed below are available on site at the Cell Center.
_ Special Ordering: NO
_ Items ordered on Friday will be available on Tuesday
_ Delivery: Overnight
PC
CODE MFGR. NO.
DESCRIPTION
OE 5102
70092Y
Alkaline Phosphatase (shrimp)
CH 2113
US12387
BCIP
PC
4110
70714
ddATP Termination Mix
PC
4111
70716
ddCTP Termination Mix
PC
4112
70718
ddGTP Termination Mix
PC
4114
70720
ddTTP Termination Mix
CH 2121
70468
Denhardt's Solution
CH 2156
19535
NBT (Nitro Blue Tetra)
PC
5241
77102
Nucleix-dATP set aq. (200uM) 0.1M
OE 5147
70710
Sequenase™ dGTP labeling mix
OE 5148
70754
Sequenase™ dGTP Nucleotide Kit
KT 5278
70170
Sequenase™ PCR Product Seq. Kit
KT 5275
70140
Sequenase™ Quick Denature Seq. Kit
OE 5150
70721
Sequenase™ Reagent Kit
OE 5151
70724
Sequenase™ Stop Solution
OE 5239
70775z
Sequenase™ v 2.0
CH 2297
US75824
Taurine
SIZE
500u
100 mg
250 µl
250 µl
250 µl
250 µl
50 ml
1g
200uM
100 µl
1 kit
1 kit
1 kit
1 kit
1.2 ml
1000u
1 kg
CC PRICE
$
85.48
$
24.34
$
25.20
$
25.20
$
25.20
$
25.20
$
37.00
$
59.94
$
148.99
$
25.20
$
119.41
$
262.92
$
184.10
$
90.93
$
17.46
$
417.91
$
115.06
INDEX OF STOCKED PRODUCTS
MR
MR
MR
MR
MR
MR
1 kb DNA Ladder
1 kb DNA Ladder
1 kb DNA Ladder
1 kb DNA Ladder - RTS - ready load
1 kb DNA Step Ladder
1 kb Plus DNA Ladder
250 µg
1000 µg
100ug
100 app
300 lns
250 µg
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 109
MR
PC
MR
MR
MR
MR
MR
MR
PC
CH
CH
MR
KT
TC
CH
MR
CH
MR
CH
OL
OL
OL
OL
OL
OL
OL
CH
CH
CH
PG
PG
PG
CH
CH
CH
CH
OL
OL
OL
OL
OL
OL
OL
OL
OL
OL
OL
MR
MR
MR
OL
OL
PG
PG
10 bp DNA Step Ladder
10 mM dNTP Mix
100 bp DNA Ladder
100 bp DNA Ladder
100 bp DNA Ladder
100 bp DNA Step Ladder
100 bp Ladder - RTS - ready load
100 bp-2kb DNA Mass Ladder
10mM dNTP Mix
10X TAE
10X TBE
123 Base Pair Ladder
1st Strand cDNA Kit
2.5S Nerve Growth Factor - Mouse Natural
20% SDS Solution
200 bp DNA Step Ladder
20X SSC Buffer
25 bp DNA Ladder
2nd Strand Buffer
3' Biotinylation 1000nm
3' Biotinylation 50/200nm
3' Fluorescein 1000nm
3' Fluorescein 50/200nm
3' Phosphorylation 1000nm
3' Phosphorylation 50/200nm
3’ Spacer C3 - 200/1000nm
30% Acrylamide/Bis 19:1
30% Acrylamide/Bis 29:1
30% Acrylamide/Bis 37.5:1
3-8% NuPAGE® Tris-Acetate Gels 1.0 mm 10 well
3-8% NuPAGE® Tris-Acetate Gels 1.0 mm 12 well
3-8% NuPAGE® Tris-Acetate Gels 1.5 mm 10 well
4-(2-Aminoethyl)Benzenesulfonyl*Fluoride Hydrochloride
40% Acrylamide/Bis 19:1
40% Acrylamide/Bis 29:1
40% Acrylamide/Bis 37.5:1
5' Amine C6 TFA
5' Biotinylation 1000nm
5' Biotinylation 50/200nm
5' Fluorescein 1000nm
5' Fluorescein 50/200nm
5' or 3' Primary Amine 1000nm
5' or 3' Primary Amine 50/200nm
5' or 3' Terminal Thiol 1000nm
5' or 3' Terminal Thiol 50/200nm
5' Phosphoryation 1000nm
5' Phosphoryation 50/200nm
50 bp DNA Ladder
50 bp DNA Ladder
50 bp DNA Ladder
6-Fam, Hex, and Tet ABI dye 1000nm
6-Fam, Hex, and Tet ABI dye 50/200nm
7% NuPAGE® Tris-Acetate Gels 1.0 mm 10 well
7% NuPAGE® Tris-Acetate Gels 1.5 mm 10 well
50 µl
1 ML
50 µg
50ug
250 µl
100 µl
100 app
200 µl
100 µl
1L
1L
100 µg
30 rxn
0.1 mg
500ML
100 µl
1L
50 µg
0.5 ml
ea
ea
ea
ea
ea
ea
ea
500 ml
500 ml
500 ml
ea
ea
ea
100 mg
500 ml
500 ml
500 ml
EA
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
200 µg
50 µg
100 lns
ea
ea
ea
ea
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 110
-ARE
MB
MB
RE
CH
CH
CH
CH
CH
CH
PC
PC
PC
SV
SV
SV
RE
RE
CH
CH
CH
OE
OE
CH
CH
CH
CH
CH
CH
CH
CH
CH
RE
TC
TC
OE
OE
RE
MS
RE
CH
CH
CH
MS
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
Aat II
ABTS® Buffer
ABTS® Tablets (50mg)
Acc I
AccuGel™ 19:1
Acetyl CoA
Acetyl CoA
Acrylamide
Acrylamide
Acrylamide
Advantage cDNA PCR Kit
Advantage cDNA Plolymerase Mix
Advantage RT for PCR
Affinity Chromatography
Affinity Column
Affinity Column
Afl II
Afl III
Agar - Bacterial Select
Agar - Granulated
Agar - Granulated
Agarase
Agarase
Agarose
Agarose
Agarose (LMP) SeaPlaque®
Agarose (LMP) SeaPlaque®
Agarose 1000
Agarose NuSieve® 3:1
Agarose NuSieve® GTG®
Agarose SeaKem® LE
Agarose SeaKem® LE
Age I
AIM V Medium
AIM V Medium
Alkaline Phosphatase (shrimp)
Alkaline Phosphatase (shrimp)
Alu I
Aluminum Foil (18” x 50ft)
AlwN I
Ammonium Persulfate
Ammonium Persulfate
Ampicillin - 25mg/ml
Amplify™
AmpliTaq Gold™ w/ buffer I - 1000u
AmpliTaq Gold™ w/ buffer I - 250u
AmpliTaq Gold™ w/ buffer I-12 pac (250u)
AmpliTaq Gold™ w/ buffer I-5 pac (1000u)
AmpliTaq Gold™ w/ buffer I-6 pac (250u)
AmpliTaq Gold™ w/ buffer II - 1000u
AmpliTaq Gold™ w/ buffer II - 250u
AmpliTaq Gold™ w/ buffer II-12 pac (250u)
AmpliTaq Gold™ w/ buffer II-5 pac (1000u)
AmpliTaq Gold™ w/ buffer II-6 pac (250u)
500u
125 ml
20 tab
500u
450 ml
10 mg
10 mg
500 g
500 g
500 g
1 ea
1 ea
ea
per run
1 ml
5 ml
2000u
250u
500 g
500 g
500 g
100u
100u
100 g
500 g
125 g
25 g
100G
125 g
125 g
500 g
125 g
250u
500 ml
1L
1000u
500u
500u
ea
500u
100 g
10 g
10 ml
1L
1000u
250u
3000u
5000u
1500u
1000u
250u
3000u
5000u
1500u
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 111
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
OE
OE
OE
MB
TC
TC
SV
SV
SV
IM
IM
CO
IM
IM
IM
RE
RE
RE
CH
MS
MS
MS
MS
MS
MS
RE
SV
RE
RE
PC
PC
SV
RE
RE
RE
TC
AmpliTaq® w/ buffer I - 1000u
AmpliTaq® w/ buffer I - 12 paq (250u)
AmpliTaq® w/ buffer I - 250u
AmpliTaq® w/ buffer I - 5 paq (1000u)
AmpliTaq® w/ buffer I - 6 paq (250u)
AmpliTaq® w/ buffer II - 1000u
AmpliTaq® w/ buffer II - 250u
AmpliTaq® w/ buffer II - 5 paq (1000u)
AmpliTaq® w/ buffer II - 6 paq (250u)
AmpliTaq® w/ buffer II- 12 paq (250u)
AmpliWax® PCR Gem 50
AmpliWax® PCR Gem 100
AMV Reverse Transcriptase
AMV Reverse Transcriptase
AMV Reverse Transcriptase
Amylose Resin
Antibiotic-Antimycotic - Pen/Strep/Fung.
Antibiotic-Antimycotic - Pen/Strep/Fung.
Antibody conjugation
Antibody precipitation with ammonium sulfate
Antibody purification (protein G)
Anti-GST Antibody
Anti-HA (12CA5)
Anti-His Antibody Selector Kit (3 x 3 µg)
Anti-Mouse Ig - HRP conjugated
Anti-Rabbit Ig - HRP conjugated
Anti-V5 Antibody
Apa I
Apa I
Apa I
Aprotinin from Bovine Lung
ART® 10 aerosol resistant pipet tip (racked)
ART® 1000E aerosol resistant pipet tip (racked)
ART® 100E aerosol resistant pipet tip (racked)
ART® 200 aerosol resistant pipet tip (racked)
ART® 20E aerosol resistant pipet tip (racked)
ART® 20P aerosol resistant pipet tip (racked)
Asc I
Ascites
Ase I
Asp 718
ATP 0.1M
ATP 0.1M
Autoclave
Ava II
Avr II
Avr II
Azaserine - 100X 0.6mM 0.1mg/ml
1000u
3000u
250u
5000u
1500u
1000u
250u
5000u
1500u
3000u
200 gem
200 gem
1000u
1000u
300u
100 ml
100 ml
20 ml
1 liter
0.5ml
200 µg
3x3ug
1 ml
1 ml
50 ul
5000u
2000u
5000u
10 ml
960/pk
800/pk
960/pk
960/pk
1000/pk
960/pk
500u
per mouse
2000u
1000u
25 µmol
40 µmol
per cycle
2000u
100u
500u
10 ml
-BRE
RE
RE
RE
RE
BamH I
BamH I
BamH I
BamH I
BamH I
2000u
2500u
2000u
10000u
50000u
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 112
RE
RE
SV
CH
CH
CH
RE
RE
MR
CH
TC
RE
RE
RE
RE
RE
RE
PC
KT
KT
FM
FM
FM
CH
PC
PC
PC
CH
CH
CH
CE
CE
CE
CE
CH
NR
CH
SV
CH
MB
CH
MB
CH
RE
RE
RE
RE
RE
RE
RE
RE
RE
BamH I
BamH I HC
Bashaw Fly Food
BCIP
BCIP
BCIP
Bcl I
Bcl I
BENCHMARK™ Prestained Protein Ladder
Bestatin
bFGF - Human Recombinant
Bgl I
Bgl II
Bgl II
Bgl II
Bgl II
Bgl II
BigDye™ Terminator Kit
BioArray High Yield RNA Transcript Labeling Kit
BioArray High Yield RNA Transcript Labeling Kit
Biomax™ Film MR-1 8x10in
Biomax™ Film MR-1 35x43cm
Biomax™ Film MS-1 8x10in
Bio-Safe Coomasie Stain
Biotin 11 CTP
Biotin 16 UTP
Biotin-16-dUTP
Bis-Acrylamide
Bis-Acrylamide
Bis-Acrylamide
BL21-Codon Plus (DE3)-RIL Competent Cells
BL21-Codon Plus (DE3)-RP Competent Cells
BL21-Codon Plus-RIL Competent Cells
BL21-Codon Plus-RP Competent Cells
Blasticidin
Blocking Reagent
BME (2-mercaptoethanol)
Bonini Fly Food
Boric Acid
Bovine Serum Albumin (Nuclease free)
BOVINE SERUM ALBUMIN MOLECULAR BIOLOGY GRADE
Bovine Serum Albumin, Acetylated
Bromophenol Blue
Bsm I
BspE I
BspH I
BssH II
BstE II
BstE II
BstE II
BstX I
Bsu36 I
2500u
10000u
3ml/150mg
100 mg
100 mg
500u
3000u
2 x 250u
10 mg
10 µg
1000u
500u
400u
2000u
10000u
500u
24 rxn
10 rxn
20 rxn
50 sht
50 sht
50 sht
1L
250nm
250nm
50 nmol
25 g
50 g
25 g
ea
ea
ea
ea
50 MG
50 g
25ml
500 g
20 mg
100g
150 mg
10g
200u
1000u
500u
200u
500u
2000u
2000u
1000u
500u
-CKT
Cal Phos Mammalian Transfection Kit
sm box
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 113
OE
OE
OE
CH
CH
NR
SV
SV
SV
SV
SV
SV
SV
TC
SV
SV
SV
NR
CH
CH
CH
NR
MS
CH
CO
CO
FM
CH
RE
RE
RE
TC
MB
FM
CH
CH
CH
PC
PC
Calf Intestinal Alkaline Phosphatase
1000u
Calf Intestinal Alkaline Phosphatase
1000u
Calf Intestinal Alkaline Phosphatase
1000u
Casamino Acids
500 g
Casamino Acids
500 g
CDP-Star Nucleic Acid Chemiluminescence Reagent (Ready to Use) 1 kit
Cell Culture ATCC line
Cell Culture ATCC shipping
Cell culture Large scale
1 liter
Cell Culture T150
Cell Culture T25
Cell Culture T75
Cell Culture–Personnel Training
Cell Dissociation Buffer - Enzyme free
100 ml
Cell Fusion w/ PEG
EA
Cell Line Flask - Set Up
Ea
Cell Line Flask - Weekly Charge
Ea
Cell Proliferation Kit (WST-1)
ea
Cesium Chloride
1 kg
Cesium Chloride
100 g
Cesium Chloride
500 g
Chemiluminescent Western Blot Kit
1000cm2
Chillete personal tube cooler -12 tubes at -20C
1 ea
Chloramphenicol 34 mg/ml
10 ml
CHROMA SPIN-10 Columns
1 ea
CHROMA SPIN-100 Columns
1 ea
Chromatography Paper 3MM 46X57cm
100/pk
Citric Acid
500 g
Cla I
500u
Cla I
1000u
Cla I
500u
Collagen - Rat Tail
100 mg
Collagenase P
100 mg
Colony/Plaque Screen™ w/orient. holes 137mm
50/bx
Complete EDTA Free 20 Tabs
EA
Complete Mini 25 Tabs
EA
Coomassie™ Brilliant Blue R-250
10g
CTP 0.1M
25 µmol
CTP 0.1M
40 µmol
-DPC
PC
PC
PC
PC
KT
PC
PC
PC
PC
PC
PC
PC
RE
dATP (100mM)
dATP 0.1M
dATP 0.1M
dATP 100mM solution
DATP PCR Grade
DC Protein Assay Kit II
dCTP (100mM)
dCTP 0.1M
dCTP 0.1M
dCTP 100mM solution
DCTP PCR Grade
ddATP Termination Mix
ddCTP Termination Mix
Dde I
40 µmol
25 µmol
25 µmol
100 µm
ea
1 kit
40 µmol
25 µmol
25 µmol
100 µm
ea
250 µl
250 µl
500u
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 114
RE
PC
PC
PC
CH
PC
CH
CH
PC
PC
PC
PC
PC
CE
CE
NR
NR
NR
CH
TC
CH
CH
CH
TC
TC
TC
TC
TC
TC
TC
TC
TC
TC
TC
TC
TC
CH
CH
SV
MR
MR
MR
MR
MR
MR
MR
OE
OE
OE
PC
OE
OE
OE
OE
OE
Dde I
200u
ddGTP Termination Mix
250 µl
ddNTP Set
4x0.5µmol
ddTTP Termination Mix
250 µl
Denhardt's Solution
50 ml
Deoxynucleotide Mix
1 vl
Dextran Sulfate
100 g
Dextran Sulfate
100 g
dGTP (100mM)
40 µmol
dGTP 0.1M
25 µmol
dGTP 0.1M
25 µmol
dGTP 100mM solution
100 µm
DGTP PCR Grade
ea
DH5alpha™ Library Efficiency Comp Cells
1 ml
DH5alpha™ Subcloning Efficiency Comp Cells
2 ml
DIG/Genius High Prime DNA Labeling and Detection Starter Kit II 1 kit
Digoxigenin RNA Labeling Mix
20 rxn
Digoxigenin-11-UTP
250 nmol
Dimethyl Sulfoxide Hybri-Max® Sterile*Filtered
25 ml
Dispase
100 ml
Dithiothreitol (DTT)
5g
Dithiothreitol (DTT)
5g
Dithiothreitol (DTT)
5g
DMEM - high glucose 25mM Hepes
500 ml
DMEM - high glucose 4.5 gm/L
1L
DMEM - high glucose 4.5 gm/L
500 ml
DMEM - high glucose 4.5 gm/L - no sodium pyruvate
500 ml
DMEM - high glucose w/o phenol red (1x)
500 ml
DMEM - low glucose
500 ml
DMEM H-glu w/ Glutamax
500ML
DMEM Nutrient Mix F12
500 ML
DMEM w/GlutaMAX
500 ML
DMEM WITH L-GLUT; HI GLUCOSE; W/ NA PYRUVATE
1L
DMEM WITH L-GLUT; HI GLUCOSE; W/O NA PYRUVATE
1L
DMEM/F-12 - 1:1 mix w/ 15mM Hepes
500 ml
DMRIE-C
1 ml
DMSO
1L
DMSO
100 ml
DNA isolation (10-20 ml blood or 100 ml culture)
DNA Marker IV (74bp - 19329bp)
50 µg
DNA Marker IX (72-1353bp)
50 µg
DNA Marker V (8-587bp)
50 µg
DNA Marker VI (154-2176bp)
50 µg
DNA Marker VIII (19bp - 1114bp)
50 µg
DNA Marker X (75-12216bp)
100 µg
DNA Marker XIV (100 - 1500 bp) 100 bp ladder
50 µg
DNA Polymerase I
500u
DNA Polymerase I from E.coli
500u
DNA Polymerase I (E Coli)
1000U
DNA Polymerization Mix
4x10umol
DNase 1
1000u
DNase I
20000u
DNase I - Amplification Grade
100u
DNase I - Grade II
100 mg
DNase I - RNase Free
10000u
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 115
OE
KT
PC
PC
PC
PC
PC
CH
CH
CH
CH
MB
CH
TC
TC
TC
TC
TC
TC
TC
TC
RE
RE
RE
SV
SV
PC
PC
PC
PC
PC
DNase RQ1 RNase-free
DNeasy Tissue Kit
dNTP Set 4x100umol
dNTP Set (4x25umol) 100mM
dNTP Set 0.1M
dNTP's (bulk) (100mM) 4x40umoles
dNTP's PCR Grade
DO Supplement-His
DO Supplement-Leu
DO Supplement-Trp
DO Supplement-Ura
DOTAP (Liposomal Transfection Reagent)
DOXYCYCLINE HYDROCHLORIDE
DPBS (10X) with Ca++ & Mg++
DPBS (10X) without Ca++ & Mg++
DPBS (10X) without Ca++ & Mg++
DPBS (1X) with Ca++ & Mg++
DPBS (1X) with Ca++ & Mg++
DPBS (1X) with Ca++ & Mg++
DPBS (1X) without Ca++ & Mg++
DPBS (1X) without Ca++ & Mg++
Dpn I
Dra I
Dra III
Drosophila medium
Drosophila medium
dTTP (100mM)
dTTP 0.1M
dTTP 0.1M
dTTP 100mM solution
DTTP PCR Grade
1000u
50
4x100µmol
EA
25 µmol
1 set
ea
1 ea
1 ea
1 ea
1 ea
2 ml
25g
500 ml
4L
500 ml
1L
1L
1L
1L
1L
1,000u
2000u
1000u
100 vials
25 bottles
40 µmol
25 µmol
25 µmol
100 µm
ea
-EOE
RE
SV
NR
NR
NR
NR
NR
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
CH
CH
E. Coli DNA Ligase
Eag I (Xma III)
EBV stock
ECL™ Plus Western Blotting Detection Reagents
ECL™ Western Blot Analysis Starter System
ECL™ Western Blotting Detection Reagents
ECL™ Western Blotting Detection Reagents
ECL™ Western Blotting Detection Reagents
EcoN I
EcoR I
EcoR I
EcoR I
EcoR I
EcoR I
EcoR I
EcoR I HC
EcoR V
EcoR V
EcoR V
EDTA 0.5M (4 x 100ml)
EDTA Disodium Salt Dihydrate
100u
500u
per tube
1 ea
1 kit
1000cm2
4000cm2
2000cm2
1000u
5000u
5000u
20000u
10000u
50000u
5000u
10000u
2000u
2000u
2000u
400 ml
500 g
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 116
CO
CE
CE
MS
MS
MS
FM
FM
OE
CO
SV
SV
TC
TC
SV
SV
SV
SV
SV
SV
SV
SV
SV
TC
CH
CH
CH
OE
OE
PC
PC
PC
PC
PC
PC
MB
KT
MB
Effectene Transfection Reagent (1 ml)
1 ml
ElectroMAX DH10B Efficiency Comp Cells
5x.1ml
ElectroMAX DH5alpha-E Efficiency Comp Cells
5x.1ml
Electroporation Cuvettes
0.1cm
Electroporation Cuvettes
0.2cm
Electroporation Cuvettes
0.4cm
Elite II Slide Film 100 - 24 Exposures
1 roll
Elite II Slide Film 100 - 36 Exposures
1 roll
Elongase Enzyme Mix
100 rxn
EndoFree Plasmid Maxi Kit (10)
kit
Endotoxin test (1-2 tests per test)
Endotoxin test (3 or more tests per test)
Epidermal Growth Factor
100 ug
Epidermal Growth Factor - Culture Grade
0.1 mg
ES Cell Culture & Karyotyping
per culture
ES Cell Culture for blastocystin injection
ES Cell Culture T150 flask
ES Cell Culture T25 flask
ES Cell Culture T75 flask
ES Cell Electroporation + Selection
fewer than 20 clones
ES Cell EP + Selection, at least 2 80% male chimera
ES Cell EP+Selection, 150-200 +ve clones, no Recombinants
ES Cell EP+Selection, at least 2 Recombinant clones
Essential Amino Acids - 50X MEM supplemt
100 ml
Ethidium Bromide (ETBR) 10mg/ml
10 ml
Ethidium Bromide Aqueous Solution 10mg/ml
10 ml
Ethidium Bromide Tablets 10mg
10 ea
Exonuclease I
2500u
Exonuclease III
5000u
Expand™ High Fidelity PCR System
100u
Expand™ High Fidelity PCR System
500u
Expand™ High Fidelity PCR System
10x250u
Expand™ Long Template PCR System
100u
Expand™ Long Template PCR System
10x250u
Expand™ Long Template PCR System
500u
Express Hyb
250 ml
Extract n Amp Red
100 rxn
ExtremeGENE (Transfection Reagent)
.4 ml
-FTC
PC
PC
CO
SM
SM
SM
SM
SM
SM
SM
TC
TC
TC
F12 Nutrient Mix, Kaighns Mod
FastStart Taq DNA Polymerase
FastStart Taq DNA Polymerase
FastTrack® 2.0 Kit
Fetal Bovine Serum
Fetal Bovine Serum, ES Qualified, Lot # A38507V
Fetal Bovine Serum, Lot # 1185797
Fetal Bovine Serum, Lot # SFB30-1491
Fetal Bovine Serum, lot #102K8410, 110% efficiency
Fetal Bovine Serum, lot #FBU2110, 145% efficiency
Fetal Bovine Serum, Lot# ANG19178
Fibronectin - Human
Fibronectin - Human
Fibronectin, human plasma
500 ML
100u
500u
6 rxn
500 ML
500 ML
500ml
500ml
500 ml
500ML
500ml
1 mg
1 mg
5mg
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 117
CH
CH
KT
MB
CH
CH
SV
TC
MB
MB
FM
FM
TC
SV
SV
SV
SV
Ficoll-Paque® Plus solution
Ficoll-Paque® solution
First Strand cDNA Synthesis Kit
fmol® DNA Sequencing System
Formamide
Formamide
Freezing Cells
Freezing Mix - 95% Calf Serum 5% DMSO
FuGENE 6 (Nonliposomal Transfection Reagent)
FuGENE 6 (Nonliposomal Transfection Reagent)
Fuji RX Film NIF 14x17in
Fuji RX Film NIF 8x10in
Fungizone
Fusion - Cell Culture Work
Fusion - Cell Culture Work
Fusion without Immunization
Fusion without Immunization
6x500ml
6x100ml
1 kit
1 kit
500 ml
500 ml
per vial
100 ml
.4 ml
1 ml
100 sht
100 sht
20ml
20 clones
40 clones
20 clones
40 clones
G418
G418
G418 Sulfate (50mg/ml)
GC Rich Polymerase
Gene Pulser® Cuvettes 0.1cm
Gene Pulser® Cuvettes 0.2cm
Gene Pulser® Cuvettes 0.4cm
GeneAmp® dNTP blend 10mM
GeneAmp® dNTP blend 10mM-12 paq
GeneAmp® dNTP blend 10mM-6 paq
GeneAmp® Core Kit
GeneAmp® dNTP Set -10mM (4x3.2umol)
GeneAmp® EZ rTth RNA PCR Kit
GeneAmp® RNA PCR Core Kit
GeneAmp® XL PCR Kit
GeneClean Turbo
GeneClean® DNA Purification Spin Kit
GeneClean® DNA Purification Spin Kit
GeneClean® II DNA Purification Kit
GeneClean® III DNA Purification Kit
Genelute Agarose Spin Column
Genescreen Plus® Hybridization Mem. 20x20cm
Genescreen Plus® Hybridization Mem. 30x45cm
Genescreen™ Hybridization Mem. 20x20cm
Geneticin (G418)®
Geneticin (G418)®
Geneticin® - (50mg/ml)
GENIUS™ 4 RNA Labeling Kit
Gentamicin
Glutamax I, 100X
Glutathione Sepharose
Glycerol
Glycine
Glycine
Glycine
1g
5g
20 ml
50 rxn
50 pk
50 pk
50 pk
1 ml
12 ml
6 ml
100 rxn
1 set
100 rxn
100 rxn
100 rxn
50 prp
50 prp
100 prp
300 prp
600 prp
70 ea
10/bx
5 sht
10/bx
5g
1g
20 ml
2x10rxn
10 ml
100ML
10 ml
500 ml
1 kg
1 kg
500 g
-GTC
TC
TC
PC
MS
MS
MS
PC
PC
PC
PC
PC
PC
PC
PC
CO
CO
CO
CO
CO
CO
FM
FM
FM
TC
TC
TC
NR
TC
TC
CH
CH
CH
CH
CH
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 118
CH
MB
MB
TC
KT
KT
PC
CH
CH
CH
Glycine
Glycogen
GONADOTROPIN FROM PREGNANT MARES' SERUM
Grace's/Hink's supp - yeastolate+lactalbumin
GST Detection Module
GST Purifications Bulk Module
GTP 0.1M
Guanidine HCL
Guanidine Isothiocyanate
Guanidine Thiocyanate
1 kg
20 mg
10000iu
500 ml
50 rxn
5 prps
25 µmol
500 g
250 g
500 g
Hae II
Ham's F-10
Ham's F-12
Ham's F-12
Ham's F-12
Hank's BSS (10X)
Hank's BSS (1X) w/o Ca++ or Mg++ or phenol
Hank's BSS 1X - w/20mM hepes/no Ca&Mg
HB101 High Efficiency Competent Cells
HBSS
HBSS w/ ca and mg
HBSS w/o ca and mg
HEPES
HEPES Buffer Solution (1M)
Herculase Enhanced DNA Polymerase
Herculase Enhanced DNA Polymerase
Hha I
Hha I
Hha I
High Prime DNA Labeling Kit
High Prime DNA Labeling mix for dCTP
High Pure Maxi Kit
High Pure PCR Template Prep
High Pure Plasmid Isolation Kit
High Pure Plasmid Isolation Kit
Hinc II
Hind III
Hind III
Hind III
Hind III
Hind III
Hind III HC
Hinf I
Hispeed Midi Kit (25) 45 minutes/200ug
Hispeed Plasmid Maxi Kit
Hispeed Plasmid Maxi Kit
Hollow Fiber cartridge setup
Hollow Fiber cartridge setup
Hollow Fiber run (5% FBS)
Hollow Fiber Run (BD Serum Free)
Horse Serum
Hot Star Taq (1000) with Q solution
2000u
500 ml
500 ml
1L
500 ml
500 ml
1L
1L
1 ml
500 ML
1L
1L
100 g
100 ml
500u
100u
1500u
2000u
1000u
50 rxn
50 rxn
10rxn
EA
50 col
250 col
1000u
5000u
10000u
5000u
10000u
5000u
10000u
5000u
45min/200ug
10 prep
25 prep
medium
large
per week
per week
500 ml
(4x250u)
-HRE
TC
TC
TC
TC
TC
TC
TC
CE
TC
TC
TC
CH
CH
PC
PC
RE
RE
RE
KT
KT
KT
KT
CO
CO
RE
RE
RE
RE
RE
RE
RE
RE
CO
KT
KT
SV
SV
SV
SV
SM
PC
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 119
PC
PC
PC
PC
RE
RE
RE
MB
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
TC
TC
TC
TC
FM
FM
FM
FM
FM
TC
TC
Hot Star Taq (250) with Q solution
HotMaster Taq w/ buffer
HotMaster Taq w/ buffer
HotMaster Taq w/ buffer
Hpa I
Hpa I
Hpa II
Human Cot-1® DNA
Hybond™ ECL™ 20x20cm
Hybond™ ECL™ 30cmx3m
Hybond™ Nylon 137mm
Hybond™ Nylon 20cmx3m
Hybond™ Nylon 22x22cm
Hybond™ Nylon 30cmx3m
Hybond™ Nylon 82mm
Hybond™ Nylon 87mm
Hybond™-C extra sup nitrocellulose 30cmx3m
Hybond™-N+ charged nylon 137mm
Hybond™-N+ charged nylon 20cmx3m
Hybond™-N+ charged nylon 20x20cm
Hybond™-N+ charged nylon 30cmx3m
Hybond™-N+ charged nylon 87mm
Hybond™-PVDF 20x20cm
Hybridoma Growth Supp - low endotoxin 10X
Hybridoma Growth Supplement - High Endotoxin 10–50X
Hygromycin B (50mg/ml)
Hygromycin B (50mg/ml)
Hypercassette™ (beige) 35x43cm
Hyperfilm MP Enveloped, 8x10in
Hyperfilm™ ECL™ 8x10in
Hyperfilm™ ECL™ 8x10in
Hyperfilm™ MP 8x10in
Hypoxanthine - 100X 10mM 1.36mg/ml
Hypoxanthine/Thymidine - 100X 10mM/1.6mM
250u
100u
250u
1000u
500u
100u
2000u
500 µg
10 sht
1 roll
50/bx
1 roll
10/bx
1 roll
50/bx
50/bx
1 roll
50/bx
1 roll
10 sht
1 roll
50/bx
10 sht
25 ml
25 ml
20 ml
20 ml
1 ea
50 sheets
25 sht
75 sht
75 sht
10 ml
50 ml
-I- -J- -KTC
TC
TC
TC
CH
CH
CH
FM
FM
FM
FM
SV
NR
TC
CH
CH
CH
SM
TC
IL-2 Human Recombinant
IL-2 Mouse Recombinant
IL-4 - Human Recombinant
IMDM w/GlutaMAX
imMedia™ Amp Blue
imMedia™ Kan Blue
imMedia™ Zeo Agar
Immobilon-NY+ 137mm DISC(Colony Plaque)
Immobilon™ P 20x20cm (Western Blot)
Immobilon™ P 26.5cmx3.75m (Western Blot)
Immun-Blot PVDF
Immunization
In Situ Cell Death Detection Kit - Fluorescein
Interleukin 2 - 10000u Human recombinant
IPTG
IPTG
IPTG
Iron Enriched Calf Serum — Lot AKH12367
Iscove's Medium
10000u
10000u/1ml
5 µg
500 ML
ea
ea
ea
50/pk
10/pk
1 roll
10 sht
per mouse
50 rxn
1 ml
1g
1g
10 g
500 ml
500 ml
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 120
TC
TC
TC
CE
MR
TC
TC
TC
OE
OE
OE
OE
OE
PC
RE
RE
RE
RE
Iscove's Medium
ITS Premix - Culture Supplement
ITS+ Premix - Culture Supplement
JM109 High Efficiency Competent Cells
Kaleidoscope Prestained Standards
Kanamycin - 100X - 10mg/ml
Kanamycin/Neomycin - 100X - 20 K/5 N mg/ml
Kennett's HY - 90% DMEM hg/10% NCTC 135
Klenow - Exo Minus
Klenow - Labeling grade
Klenow - Sequencing Grade
Klenow - Sequencing Grade
Klenow Fragment
Klentaq LA DNA Polymerase
Kpn I
Kpn I
Kpn I
Kpn I HC
500 ml
5 ml
20 ml
1 ml
500 µl
10 ml
25 ml
1L
100u
100u
100u
200u
150u
500u
5000u
3000u
2500u
2000u
Lambda DNA
Lambda DNA/HindIII Fragment
Lambda DNA/HindIII Fragment
Lambda DNA/HindIII Fragment
Lambda DNA/HindIII Fragment - RTS - ready load
Lambda Quick! Phage DNA Isolation Spin Kit
Laminin - Mouse
LB + Ampicillin (50ug/ml) Agar Plates
LB + Kanamycin (50ug/ml) Agar Plates
LB + Tetracycline (20ug/ml) Agar Plates
LB Agar
LB Agar - EZMix®
LB Agar - Miller
LB Agar - Miller
LB Agar Plates
LB BROTH
LB Broth - EZMix®
LB Broth - Lennox
LB Broth - Lennox
LB Broth - Miller
LB Broth - Miller
LB Medium Capsules
LC FastStart DNA Mstr Sybrgreen
LC FastStart DNA Mstr Sybrgreen
Leupeptin
Leupeptin Hemisulfate from Microbial Source
L-Glutamine - 100X - 200mM - 29.23mg/ml
L-Glutamine - 200mM
L-Glutamine - 200mM
LipofectAMINE 2000
LipofectAMINE 2000
Lipofectamine™
Lipofectamine™
Lipofectamine™
Lipofectin® Reagent
250 µg
500 µg
150 µg
100µg
100 app
25 prp
1 mg
10 pl
10 pl
10 pl
500 g
6 x 500 ml
500 g
500 g
10 pl
1L
1 kg
500 g
500 g
500 g
500 g
1 kg
EA
EA
25 mg
10 mg
100 ml
100 ml
100 ml
1.5ml
0.75ml
1 ml
4 x 1 ml
.5 ml
1 ml
-LMR
MR
MR
MR
MR
CO
TC
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
PC
PC
CH
CH
TC
TC
TC
MB
MB
MB
MB
MB
MB
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 121
SV
NR
NR
NR
NR
NR
SV
SV
Liquid N2 storage (per vial/quarter)
Luciferase Assay System
Luciferase Assay System (10 pk) quote#5075
Luciferase Assay System–10 pack
Lumi Light
Lumi Light +
Lymphocyte EBV Transformation
Lymphocyte isolation
100 assays
1000 assays
1000 assays
1 kit
1 kit
-MMB
RE
RE
MR
MR
FM
FM
CH
OE
OE
OE
MR
PC
PC
PC
PC
TC
TC
TC
TC
TC
CE
CE
CE
RE
RE
TC
TC
TC
TC
TC
TC
TC
TC
RE
CO
CO
CO
CO
CO
MS
MS
CH
CH
m7GpppG 5'G RNA Cap Structure
Mae I
Mae II
MagicMark Western Protein Standard
MagicMark XP
Magna 137mm Notched
Magnagraph 137mm
Magnesium Chloride
Maloney-MLV Reverse Transcriptase
Maloney-MLV Reverse Transcriptase
Maloney-MLV Reverse Transcripts
Mark12™ Wide Range Molecular Weight Standard
MasterMix
MasterTaq Kit
MasterTaq Kit
MasterTaq Kit
Matrigel® Growth Factor Reduced
Matrigel® Matrix
Matrigel® Phenol Free & Growth Fact Reduced
Matrigel® Phenol Red Free
Matrisperse® Cell Recovery Solution
Max Efficiency DH5@T1 Competent Cells
MAX Efficiency DH5alpha Efficiency Comp Cells
Max Efficiency STBL2
Mbo I
Mbo I
McCoy's 5A
Medium 199
MEM - w/ Earles' salts & L-Glut
MEM - w/ Earles' salts & L-glut
MEM - w/ Earles' salts & L-Glut
MEM alpha - w/ nucleosides
MEM alpha - w/o nucleosides
MEM alpha - w/o nucleosides
Mfe I
Micro-FastTrack™ Kit
MicroSpin G-25 Columns
MicroSpin G-50 Columns
MicroSpin S-200 HR Columns
MicroSpin S-300 HR Columns
Mini-Centrifuge
Mini-Centrifuge 6 x 1.5 ml
Minimal SD Agar Base
Minimal SD Base
25u
50u
ea
250ul
250ul
50/pk
50/pk
500 g
200u
40000u
10000u
1.0 ml
152u
100u
250u
500u
10 ml
10 ml
10 ml
10 ml
100 ml
1 ml (5 x 0.2ml)
5x.1ml
1 ML
500u
200u
500 ml
500 ml
1L
1L
1L
1L
1L
500 ml
500u
20 rxn
50 col
50 col
50 col
50 col
ea
1 ea
1 ea
1 ea
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 122
RE
RE
RE
RE
MR
OE
IM
SV
SV
SV
SV
Mlu I
Mlu I
Mnl I
Msc I
MultiMark® Multi-Colored Standard
Mung Bean Nuclease
myc 1 - 9E10 antibody
Mycoplasma Enzymatic test, MycoAlert
Mycoplasma Enzymatic test, MycoAlert
Mycoplasma test PCR (1-2 samples per sample)
Mycoplasma test PCR (3 or more samples per sample)
1000u
1000u
250u
100u
500 µl
3000u
0.5 mg
1-5 samples
> 5 samples
N2 Supplement
Nae I
Nar I
NBT (Nitro Blue Tetra)
NBT (Nitro Blue Tetra)
Nco I
Nco I
Nco I
Nde I
Nde I
Neural Supplement - B27 (50X)
Neuralbasal Media
Nhe I
Nhe I
Nhe I
Nick translation Kit
NICK® Columns
Ni-NTA Superflow
Nitrobind 7x8.4
Nitrobind Pure 137mm
Nitrobind Pure 20cmx3m
Nitrobind Pure 30cmx3m
Nitrocellulose 82mm Colony & Plaque
Nitrocellulose 137mm Colony & Plaque
Nitrocellulose Membrane (.20u) 13.5x16.5cm
Nitrocellulose Membrane (.20u) 7x8.4cm
Nitrocellulose Membrane (.45u) 33cmx3m
Nitrocellulose Membrane (0.2 µm) Filter Paper Sandwich
Nitrocellulose Membrane (0.45 µm) Filter Paper Sandwich
Nitropure Supp 82mm
Nitropure Supp 30cmx3m
Non-essential amino acids - 100X
Not I
Not I
Not I
Not I
Not I
NP Transfer Buffer (20X)
Nru I
Nru I
Nsi I
5 ml
500u
200u
3 ml
1g
200u
1000u
200u
4000u
500u
10 ml
500 ml
1000u
5000u
250u
50 rxn
50 col
25 ml
10/pk
50/pk
1 roll
1 roll
50/pk
50/pk
10 sht
10 sht
1 roll
20/pk
20/pk
50/pk
1 roll
100 ml
200u
200u
500u
2500u
200u
1L
1000u
200u
1000u
-NMB
RE
RE
CH
CH
RE
RE
RE
RE
RE
TC
TC
RE
RE
RE
KT
CO
CO
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
FM
TC
RE
RE
RE
RE
RE
CH
RE
RE
RE
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 123
PC
PC
PG
PG
PG
PG
PG
PG
PG
PG
PG
PG
CH
CH
CH
CH
CH
CH
CH
TC
NTP Set 0.1M
Nucleix-dATP set aq. (200uM) 0.1M
NuPAGE 12% Bis-Tris Gels 1.0mm 12 well
NuPAGE 4-12% Bis-Tris Gels 1.5mm 10 well
NuPAGE® 10% Bis-Tris Gels 1.0 mm 10 well
NuPAGE® 10% Bis-Tris Gels 1.0 mm 12 well
NuPAGE® 10% Bis-Tris Gels 1.0 mm 15 well
NuPAGE® 12% Bis-Tris Gels 1.0 mm 10 well
NuPAGE® 12% Bis-Tris Gels 1.0 mm 15 well
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 10 well
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 12 well
NuPAGE® 4-12% Bis-Tris Gels 1.0 mm 15 well
NuPAGE® Antioxidant
NuPAGE® LDS Sample Buffer (4X)
NuPAGE® MES SDS Running Buffer (20X)
NuPAGE® MOPS SDS Running Buffer (20X)
NuPAGE® Sample Reducing Agent (10X)
NuPAGE® Transfer Buffer (20X)
NuPAGE® Tris-Acetate SDS Running Buffer (20X)
Nutridoma® SP
4x25µmol
200uM
ea
EA
ea
ea
ea
ea
ea
ea
ea
ea
15ml
10ml
500ml
500ml
250ul
125ml
500ml
10 ml
-O- -PMB
MS
MS
MB
MB
CE
CE
CE
CE
CE
TC
TC
TC
MS
MS
MS
MS
MS
RE
MB
KT
PC
PC
PC
PC
KT
CH
CH
TC
TC
TC
TC
Oligo (dT) 12-18 Primer
Oligo CD - $1,000
Oligo CD - $500
Oligo(dT) 15 primer
Oligofectamine Reagent
One Shot MACH 1 Comp eColi (LTI Shelf)
One Shot® MAX Efficiency® DH5a®-T1®
OneShot™ INVaF' Competent Cells
OneShot™ TOP10 Competent Cells
OneShot™ TOP10F' Competent Cells
OPI 100X - 15mg O - 5 mg P - 20u I/ml
Opti-MEM® - reduced serum hybrid. medium
Origen® Hybridoma Cloning Factor - 10-50X
P-100 Filter Pipet Tips
P-1000 Filter Pipet Tips
P2/P10 Eatra Long Filter Pipet Tip
P-20 Filter Pipet Tips
P-200 Filter Pipet Tips
Pac I
pBR322/Msp I Digest
pcDNA3.1© Directional TOPO® Expression Kit (Top 10 Cells)
PCR Cleanup Kit
PCR Markers
PCR Nucleotide Mix
PCR Optimization Kit (for optimal pH & Mg)
pCR-Script™ Amp Cloning Kit, (3 parts, -80,-20, RT)
PDA
Pefabloc® SC
PEG(50%+5%DMSO) - hi efficiency/fusion
Pen/Strep - 100X -10000u/ml P - 10mg/ml S
Pen/Strep - 100X -10000u/ml P - 10mg/ml S
Pen/Strep - 100X -10mcg/ml P - 10mg/ml S
25 UG
EA
EA
20 µg
1 ML
20 rxn
20 x 50ul
21x50µl
21x50µl
21x50µl
50 ml
500 ml
50 ml
EA
EA
EA
EA
EA
250u
50 µg
20 rxn
100 rxn
250 µl
100 rxn
1 kit
1 kit
10 g
100 mg
2 ml
100 ml
100 ml
100 ml
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 124
CH
CH
CH
CH
CH
CH
TC
TC
PC
PC
PC
PC
PC
PC
MB
MB
MB
CH
CH
CH
CH
CH
CH
CH
CH
CH
MR
MR
MR
MR
MR
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
MS
PC
PC
PC
PC
PC
PC
PC
PC
MB
RE
FM
FM
Pepstatin (was #600160)
Peptone
Peptone
Percoll®
Perfect Hyb Plus Hybridization Buffer
Perfect Hyb Plus Hybridization Buffer
PFHM II - Protein Free Hybridoma Medium
PFHM II - Protein Free Hybridoma Medium
Pfu DNA Polymerase
Pfu DNA Polymerase
Pfu Ultra High Fidelity
PfuTurbo™ DNA Polymerase
PfuTurbo™ DNA Polymerase
PfuTurbo™ HotStart DNA Polymerase
pGEM®-T Easy I
pGEM®-T Easy II
pGEM®-T Vector I
Phenol
Phenol Saturated (Buffer)
Phenol Saturated (Tris)
Phenol (was 100300)
Phenol Saturated pH 6.6
Phenol Saturated pH 6.6
Phenol/Chloroform/Isoamyl Alcohol
Phenol/Chloroform/Isoamyl Alcohol
Phenylmethylsulfonyl Fluoride
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment
Phi-X174 DNA/Hae III Fragment - RTS - ready load
Pipetman 0.1-2.0µl
Pipetman 0.5-10µl
Pipetman 100-1000µl
Pipetman 10-100µl
Pipetman 2-20µl
Pipetman 50-200µl
Pipet-mate Digital Pipette w/ tip ejector 10µl
Pipet-mate Digital Pipette w/ tip ejector 100µl
Pipet-mate Digital Pipette w/ tip ejector 1000µl
Pipet-mate Digital Pipette w/ tip ejector 20µl
Pipet-mate Digital Pipette w/ tip ejector 200µl
Pipet-mate Digital Pipette w/ tip ejector 5000µl
Platinum Pfx DNA Polymerase
Platinum Pfx DNA Polymerase
Platinum Pfx DNA Polymerase
Platinum Supermix Hi Fi
Platinum Taq DNA Polymerase - 100u
Platinum Taq DNA Polymerase - 250u
Platinum Taq DNA Polymerase - 500u
Platinum Taq High Fidelity
Plus Reagent
Pme I
Polaroid Film Type 57
Polaroid Film Type 667
10 mg
500 g
500 g
1L
125 ml
1L
1L
500 ml
100u
500u
100 u
100u
500u
100u
1 kit
1 kit
20u
500 g
100 ml
100 ml
500 g
100 ml
400 ml
100 ml
400 ml
5g
40 µg
250u
50u
50 µg
100 app
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
1 ea
100 u
250 u
500 u
100 rxns
100u
250u
500u
100u
.85 ml
500u
pk
pk
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 125
MB
CO
FM
CH
MR
MR
MR
MR
MR
KT
MB
MB
MB
MB
MB
MB
MB
MB
MB
MB
CO
CH
CH
CH
CH
CH
CH
CH
CH
MB
IM
KT
KT
IM
CH
CH
CH
CH
CH
CH
CH
CH
CH
RE
RE
RE
KT
FM
RE
RE
RE
RE
RE
RE
Poly d(I-C)
PolyATtract® System 1000 RNA Isolation System
Polyscreen® PVDF 30cmx3m
Potassium Chloride
Precision Plus Protein Dual Color Standard
Prestained Protein Molecular Weight Marker
Prestained Standards Broad
Prestained Standards High
Prestained Standards Low
Prime-It® II Random Priming Kit
Primer gt10 forward
Primer gt10 reverse
Primer gt11 forward
Primer gt11 reverse
Primer M13/pUC Forward 23mer
Primer M13/pUC Reverse 23mer
Primer Sp6 20mer
Primer T3 20mer
Primer T7 20mer
Primer T7 Term
ProbeQuant™ G-50 Micro Columns
Protease Inhibitor Cocktail Tablets (Complete)
Protease Inhibitor Cocktail-Bacterial
Protease Inhibitor Cocktail-Bacterial
Protease Inhibitor Cocktail-General
Protease Inhibitor Cocktail-Mammalian w/o Metal Chelators
Protease Inhibitor Cocktail-Mammalian w/o metal chelators
Protease Inhibitor Cocktail-Poly His Tagged
Protease Inhibitor Cocktail-Yeast/Fungal
Protector RNase Inhibitor
Protein A Agarose
Protein Assay Kit Dye Reagent Concentrate
Protein Assay Kit II
Protein G Agarose
Proteinase K
Proteinase K
Proteinase K
Proteinase K
Proteinase K
Proteinase K Solution (20 mg/ml)
ProtoGel® (30% Acrylamide/0.8% Bis)
ProtoGel® Buffer
ProtoGel® Stacking Buffer
Pst I
Pst I
Pst I
PureTaq RTG PCR Beads
PVDF Membrane/Filter Paper Sandwich
Pvu I
Pvu I
Pvu I
Pvu II
Pvu II
Pvu II
10 Å260u
1 kit
1 roll
500 g
50 app
175 lns
500 µl
500 µl
500 µl
30 rxn
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
5 µg
50 col
20 tabs
5 ml
25 ml
1 btl
1 ml
5 ml
5 ml
1 ml
EA
5 ml
1 kit
1 kit
5 ml
100 mg
25 ml
500 mg
100 mg
500 mg
5 ml
1L
450 ml
200 ml
3000u
10000u
3000u
0.5ML
20/pk
150u
100u
100u
1000u
5000u
1000u
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 126
-QCO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
PC
CO
CO
CO
CO
CO
CO
CO
CO
KT
MB
QIAamp™ Blood Kit
QIAamp™ DNA Mini Kit
QIAexpress™ Ni-NTA Agarose Kit
QIAEX™ II Gel Extraction Kit
QIAGEN Plasmid Giga Kit
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Maxi Kit
QIAGEN Plasmid Mega Kit
QIAGEN Plasmid Midi Kit
QIAGEN Plasmid Mini Kit
QIAprep Spin Miniprep Kit
QIAprep™ Spin Miniprep Kit
QIAquick 8 PCR Purification Kit
QIAquick Gel Extraction Kit
QIAquick™ Gel Extraction Kit
QIAquick™ Nucleotide Removal Kit (50)
QIAquick™ PCR Purification Kit
QIAshredder™
QuantiTect SYBR Green PCR Kit
Quantum Maxi-Prep Kit
Quantum Midi-Prep Ket
Quantum™ Prep Plasmid Miniprep
Quick Spin™ G25 DNA
Quick Spin™ G25 RNA
Quick Spin™ G50 DNA
Quick Spin™ G50 DNA
Quick Spin™ G50 RNA
QuickChange™ Site-Directed Mutagenesis Kit
QuickHyb® Rapid Hybridization Buffer
50 prp
50 prp
25 ml
150 prp
5 prp
10 col
25 col
100 prp
5 col
25 col
25 col
250 prp
50 rxn
400 prp
250 prp
50 prp
kit
50 rxn
50 cap
200 rxn
10 prp
20 prp
kit
20 col
20 col
20 col
50 col
20 col
1 kit
250 ml
Rabbit Reticulocyte Lysate
Rad Prime Labeling Kit
Rainbow Marker™ (14300-250000)
Rainbow Marker™ (2350-46000)
Random Primed DNA Labeling Kit
Random Primers (N)6
Random Primers (N)6
Rapid Gel Extraction Kit
Rapid PCR Purification Kit
Rapid Plasmid Mini Prep
RapidHyb Hybridization™ Buffer
Ready Load 1KB Plus DNA Ladder
ReadyGel, 10% Tris-Hcl, 10 well
ReadyGel, 10% Tris-Hcl, 50ul
ReadyGel, 4-15% Tris-Hcl, 10 well
ReadyGel, 4-15% Tris-Hcl, 50ul
Ready-To-Go DNA Labelling Beads (-dCTP)
Rediprime™ kit for dCTP
Redtaq polymerase
Renaissance™ Nucleic Acid Chemilum Rgt Kit
Renaissance™ Western Blot Chemilum Rgt
Renaissance™ Western Blot Chemilum Rgt
1 ml
30 rxn
250 µl
250 µl
50 rxn
50 Å260u
50 Å260u
50rxn
50rxn
50rxn
125 ml
100 rxn
EA
EA
EA
EA
1 kit
30 rxn
250 unit
1 kit
1000cm2
2500cm2
-RMB
KT
MR
MR
KT
MB
MB
KT
KT
KT
MB
MR
PG
PG
PG
PG
KT
OE
PC
NR
NR
NR
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 127
NR
OE
OE
MR
MB
OE
OE
MB
OE
OE
MB
MB
MB
MB
CO
CO
TC
TC
TC
TC
TC
TC
TC
CO
RE
PC
PC
PC
Renaissance™ Western Blot Chemilum Rgt
Ribonuclease H
Ribonuclease H
RNA Ladder 0.24-9.5 kb
RNAguard Ribonuclease Inhibitor
RNase - DNase free
Rnase A
RNase AWAY
RNase H
RNase T1
RNasin® Recombinant Ribonuclease Inhibitor
RNasin® Recombinant Ribonuclease Inhibitor
RNasin® Ribonuclease Inhibitor
RNasin® Ribonuclease Inhibitor
RNeasy Mini Kit
RNeasy™ Mini RNA Kit
RPMI 1640
RPMI 1640
RPMI 1640
RPMI 1640 - no phenol
RPMI 1640 - w/ 25mM Hepes
RPMI 1640 - w/ 25mM Hepes
RPMI w/GlutaMAX
RPM™ Rapid Pure Plasmid Miniprep Kit
Rsa I
rTth DNA Polymerase & EZ Buffer Pack
rTth DNA Polymerase with Buffer
rTth DNA Polymerase-XL w/XL Buffer Pack
5000cm2
30u
50u
75 µg
5000u
500 µg
100 mg
8.4 oz
250u
100000u
10000u
2500u
10000u
2500u
250 prp
50 prp
1L
1L
1L
500 ml
500 ml
500 ml
500 ML
120 prp
1000u
100 rxn
100 rxn
100 rxn
-STC
OE
RE
RE
RE
RE
RE
RE
RE
RE
RE
MB
RE
RE
RE
RE
RE
CH
MR
MR
CH
CH
CH
CH
S.O.C. Transformation Media - 10x10 ml
S1 Nuclease
Sac I (Sst I)
Sac I (Sst I)
Sac I (Sst I)
Sac II
Sac II
Sal I
Sal I
Sal I
Sal I
Salmon Sperm DNA
Sap I
Sau 3A I
Sau 3A I
Sca I
Sca I
SDS 10% (4x100ml)
SeeBlue® Plus2 Pre-Stained Standard
SeeBlue® Pre-Stained Standard
SequaGel® Buffer
SequaGel® Concentrate (20% Acryl.) 19:1
SequaGel® Diluent
SequaGel® Sequencing System
100 ml
10000u
1000u
2000u
1000u
2000u
500u
500u
2000u
10000u
2000u
10 x 1ml
50u
200u
100u
500u
1000u
400 ml
500 µl
500 µl
200 ml
1L
1L
1 kit
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 128
CH
CH
OE
OE
KT
KT
OE
OE
OE
FM
FM
TC
TC
RE
RE
NR
KT
MR
RE
RE
RE
RE
RE
RE
TC
CH
CH
CH
TC
TC
OE
OE
OE
OE
RE
RE
RE
SV
SV
SV
RE
RE
RE
RE
CH
CH
RE
RE
CH
RE
FM
FM
FM
FM
FM
SequaGel®-6 6% Monomer Sequencing Soln (includes EC-841) 1 L
SequaGel®-8 8% Monomer Sequencing Soln (includes EC-841) 1 L
Sequenase™ dGTP labeling mix
100 µl
Sequenase™ dGTP Nucleotide Kit
1 kit
Sequenase™ PCR Product Seq. Kit
1 kit
Sequenase™ Quick Denature Seq. Kit
1 kit
Sequenase™ Reagent Kit
1 kit
Sequenase™ Stop Solution
1.2 ml
Sequenase™ v 2.0
1000u
Sequi-Blot™ PVDF Membrane (.20u) 15x15cm
10 sht
Sequi-Blot™ PVDF Membrane (.20u) 7x8.4cm
10 sht
SF900 II - Serum-free insect medium
500 ml
SF900 II - Serum-free insect medium
1L
Sfi I
250u
Sfi I
2000u
Sheep Anti-digoxigenin-AP
150u
Silver Stain Plus Kit
kit
SimplyBlue™ SafeStain
1L
Sma I
1000u
Sma I
1000u
Sma I
2000u
Sma I
1000u
SnaB I
500u
SnaB I
100u
Sodium Bicarbonate
100 ml
Sodium Dodecyl Sulfate
100 g
Sodium Dodecyl Sulfate (SDS)
500 g
Sodium Dodecyl Sulfate (SDS)
100g
Sodium Pyruvate - 100X 11g/l 100mM
100 ml
Sodium Pyruvate, 100mM solution
100 ml
SP6 RNA Polymerase
3000u
Sp6 RNA Polymerase
500u
Sp6 RNA Polymerase
1000u
SP6 RNA Polymerase (25u/µl)
10000u
Spe I
200u
Spe I
2500u
Spe I
200u
Special Media (10L or more powder provided)
1 liter
Special Media (10L or more)
1 liter
Special Media (less than 10L)
1 liter
Sph I
200u
Sph I
200u
Sph I
250u
Sph I
200u
SSC 20X
4L
SSC 20X
4L
Ssp I
1000u
Ssp I
500u
SSPE 20X
4L
Sst I
2000u
Stericup Filtr. & Stor.-PES 250ml .22µ
12/pk
Stericup Filtr. & Stor.-PES 500ml .22µ
12/pk
Stericup Filtr. & Stor.-PES 1000ml .22µ
12/pk
Stericup Filtr. & Stor.-PES 150ml .22µ
12/pk
Steriflip-GP Filter Unit 50µl
25/pk
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 129
SV
FM
FM
FM
FM
IM
RE
RE
RE
RE
CH
CH
CO
OE
KT
OE
OE
PC
OE
PC
OE
CE
CE
RE
PC
PC
PC
FM
FM
FM
FM
Sterile Hood Usage
Steritop-GP Filtr 1000 ML 33 mm neck 0.22µm
Steritop-GP Filtr 1000 ML 45 mm neck 0.22µm
Steritop-GP Filtr 500 ML 33 mm neck 0.22µm
Steritop-GP Filtr 500 ML 45 mm neck 0.22µm
Streptavidin Red 670™
Stu I
Stu I
Sty I
Sty I
Sucrose
Sucrose
SuperFect Transfection Reagent (1.2 ml)
Superscript Custom Choice Kit
Superscript First-Strand Synthesis System for RT-PCR
Superscript III Reverse Transcriptase
SuperScript III, 1 step w/Platinum Taq 100
SuperScript One Step RT-PCR with Platinum Taq
Superscript™ II Reverse Transcriptase
Superscript™ II Reverse Transcriptase
Superscript™ Reverse Transcriptase
SURE® 2 Supercompetent Cells
SURE® Competent Cells
Swa I
Sybr Green PCR Master Mix
Sybr Green PCR Master Mix
SYBR Green qPCR
Syringe fltr-Durapore PVDF/PVC .22µ
Syringe fltr-Durapore PVDF/PVC .45µ
Syringe fltr-mixed esters of cellulose .22µ
Syringe fltr-mixed esters of cellulose .45µ
per hour
12/pk
12/pk
12/pk
12/pk
100 µg
1000u
400u
3000u
2000u
1 kg
1 kg
3 mg/ml
10 rxn
50 rxn
10000u
100 rxn
25 rxn
10000u
4 x 10000u
10000u
ea
5x0.2ml
200u
pk
10 pk
100 rxn
50/pk
50/pk
50/pk
50/pk
-TOE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
OE
KT
T3 RNA Polymerase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase
T4 DNA Ligase HC
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Polymerase
T4 DNA Rapid Ligation Kit (5 minutes)
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase FPLCpure
T7 RNA Polymerase
TA Cloning Kit® with Dual Promoters (INVaF')
1000u
100u
100u
500u
20000u
100000u
100u
20000u
100u
50u
250u
150u
100u
40 rxn
200u
200u
500u
2500u
100u
200u
1000u
20 rxn
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 130
CH
MB
MB
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
PC
CH
CH
TC
TC
TC
CH
CH
OE
OE
OE
CH
CH
MB
TC
TC
PC
PC
PC
OE
PC
PC
IM
KT
KT
KT
KT
KT
KT
KT
KT
KT
KT
KT
SV
TAE 10X
Talon Metal Affinity Resin
Talon Superflow Metal Affinity Resin
Taq DNA Polymerase
Taq DNA Polymerase
Taq DNA Polymerase
Taq DNA Polymerase
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer
Taq DNA Polymerase w/ buffer A
Taq DNA Polymerase w/ buffer B
Taq DNA Polymerase w/10X Buffer
Taq PCR Core Kit
TaqMan Universal Master Mix
TaqMan Universal Master Mix
Taqstart Antibody
Taurine
TBE 10X
TE 10X, pH 7.4
TE, 1X Solution, pH 7.4
TE, 1X Solution, pH 8.0
TEMED
TEMED
Terminal Deoxy Transferase (TdT)
Terminal Deoxy Transferase (TdT)
Terminal Deoxy Transferase (TdT)
Terrific Broth (Modified)-EZMix®
Tetracycline 12.5mg/ml
Tfx-10 Transfection Reagent
TGF-alpha - Human Recombinant
TGF-beta Human Natural
Tgo DNA Polymerase
Tgo DNA Polymerase
Tgo DNA Polymerase
Thrombin Protease
TITAN™ One-Tube RT-PCR System
TITAN™ One-Tube RT-PCR System
TNF-a
TNT®-SP6 Coupled Transcription/Translation
TNT®-SP6/T7 Coupled Transcription/Translation
TNT®-T3 Coupled Transcription/Translation
TNT®-T7 Coupled Transcription/Translation
TNT®-T7 QUICK Coupled Transcription/Translation
TNT®-T7/T3 Coupled Transcription/Translation
TOPO TA Cloning® Kit (TOP10 Cells)
TOPO TA Cloning® Kit (TOP10F' Cells)
TOPO TA Cloning® Kit (with LTI 4622 - DH5a Cells)
TOPO TA Cloning® Kit Dual Promoter (TOP10F' Cells)
TOPO TA Cloning® Kit for Sequencing (Top 10 Cells)
Transition to protein free medium
4L
10 ml
25 ml
250u
10x250u
250u
2500u
100u
250u
5000u
100u
500u
100u
500u
100u
100u
250u
100 rxn
200 rxn
2000 rxn
200 tst
1 kg
1L
1L
1L
1L
50 g
5 ml
2500u
500u
300u
6 x 500ml
10 ml
1 ea
50 µg
1 µg
50 rxns
100 rxns
250 rxns
500u
100 rxn
25 rxn
10µg
1 kit
1 kit
1 kit
1 kit
1 kit
1 kit
20 rxn
20 rxns
20 rxns
1 ea
20 rxn
per line
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 131
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
CH
MB
OE
CH
TC
TC
TC
TC
TC
CH
CH
NR
NR
CH
Tri Reagent
Tri Reagent
Tris
Tris
Tris
TRIS pH 7.5
TRIS pH 8.0
Tris Base
TRIS EDTA Solution 100X
Tris Hydrochloride
TRIS/Glycine/SDS 10X Buffer
Tris-Base
Tris-Glycine (10X)
Triton® X-100
TRIZMA® Base Molecular Biology Reagent Grade
TRIZMA® Base Reagent Grade
TRIZMA® Hydrochloride Reagent Grade
Trizma-Hcl, 1 M Stock Solution, pH 7.6
Trizma-Hcl, 1 M Stock Solution, pH 8.0
Trizol
TRIzol Reagent
tRNA
Trypan Blue Solution
Trypsin - 2.5% (10X)
Trypsin-EDTA - 0.05% T - .53mM EDTA
Trypsin-EDTA - 0.05% T (1X)
Trypsin-EDTA - 0.25% (1X)
Trypsin-EDTA - 0.5% (10X)
Tryptone
Tryptone Peptone
TUNEL Enzyme (optimized terminal transferase)
TUNEL Label (FITC dUTP + dNTPs)
Tween 20
25 ml
100 ml
1 kg
500 g
1 kg
1L
1L
1 kg
1L
500 g
1L
500 g
1L
100 ml
1 kg
1 kg
1 kg
1L
1L
200 ml
100 ml
100 mg
100ML
100 ml
100 ml
100 ml
100 ml
100 ml
500 g
500 g
1 ea
1 ea
100 ml
-U- -V- -WCH
CH
PC
PC
PC
TC
TC
TC
CH
IM
CO
CO
CO
CO
CO
CO
CO
CO
CO
CO
Urea
Urea
UTP 0.1M
Vent® (exo-) Polymerase
Vent® DNA Polymerase
Versene - 0.02% EDTA in HBSS
Vitamin Mixture - 100X vitamin supplement
Water Sterile - Tissue Culture Grade
Water(Sterile Nuclease Free)
Western Blue Stabilized Substrate for AP
Wizard™ DNA Clean-up System
Wizard™ Lambda System
Wizard™ Maxipreps System
Wizard™ Megapreps System
Wizard™ Midipreps System
Wizard™ Minicolumns
Wizard™ Miniprep DNA Purification Resin
Wizard™ PCR Preps System
Wizard™ Plus Miniprep System
Wizard™ Plus Miniprep System
500 g
2 kg
25 µmol
200u
200u
1L
100 ml
1L
1L
1 ea
100 col
20 col
10 col
5 col
25 col
250 col
250 ml
50 col
50 col
250 col
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 132
CO
CO
MS
Wizard™ Plus SV
Wizard™ Plus SV
Wrapping Film (18in x 1000ft)
50 rxn
250 rxn
ea
-X- -Y- -ZRE
RE
RE
RE
RE
RE
RE
CH
CH
CH
RE
RE
RE
RE
RE
CE
CE
CE
CE
CE
RE
RE
FM
FM
FM
FM
FM
CH
CH
MB
MB
MB
CH
MB
KT
FM
Xba I
Xba I
Xba I
Xba I
Xba I
Xba I
Xba I
X-gal
X-gal
X-gal
Xho I
Xho I
Xho I
Xho I
Xho I HC
XL 10-Gold Ultracompetent Cells
XL-1 Blue Electroporation Competent Cells
XL10-Gold Ultracompetent Cells
XL-1-Blue Supercompetent Cells
XL-2 Blue Ultra Competent Cells
Xma I
Xmn I
X-Omat™ AR-2 interleaved film 35x43cm
X-Omat™ AR-2 interleaved film 8x10in
X-Omat™ AR-5 non-interleaved film 8x10in
X-Omat™ Blue 5x7
X-Omat™ Blue 8x10in
Yeast Extract
Yeast Extract
Yeast tRNA
Yeastmaker Carrier DNA
Yeastmaker Yeast Transformation System
YT Broth 2X
Zeocin
Zero Blunt® TOPO® PCR Cloning Kit (TOP 10 Cells)
Zeta-Probe® GT Membrane15x15cm
1500u
1000u
2000u
10000u
3000u
15000u
2000u
1g
250 mg
1g
5000u
25000u
3000u
5000u
2500u
10 x 100µl
5x80µl
5x100ul
5x0.2ml
ea
250u
1000u
50 sht
50 sht
50 sht
100/bx
100/bx
500 g
500 g
25 mg
ea
kit
500 g
1g
20 rxn
15 sht
University of Pennsylvania Department of Genetics Core Facilities Catalog 2004 – page 133