Connexin 26 Exon 1 Primers
Homo sapiens connexin 26 gene, exon 1.
ACCESSION
AF144321
VERSION
AF144321.1 GI:5019930
CX26-Exon1F 5'- GGCGACACCACAAACCTC -3'
CX26-Exon1R 5'- CCTCCGTAACTTTCCCAGTC -3'
PCR
30 ul Reaction
1X
PCR Mix
15
Primer F (10mM)
2
Primer R(10mM)
2
DNA
1
H2O
9
DMSO
1
(Program: T.D. 55-45 HASHEM)
Cx26-Exon1 mutation WT
540 base pairs
Table by enzyme name
Table by Enzyme Name
Enzyme
name
BspMI
No. Positions
cuts of sites
1
309
Recognition
sequence
acctgc
Enzymatic digestion with BspMI
BspMI:
10X Buffers 3:
D.D. H2O:
PCR product
1.5 l
2.0 l
12.5 l
4.0 l
We multiply each portion by the number of samples that we are doing.
 Add a known heterozygote to the digestion reaction, for control.
 Tubes are incubated at 37 oC for 8 hours.
 We load 5 l of digested reaction on a 2% agarose gel.
Expected Fragments
More info
Wildtype
Heterozygote
Homozygote
540
540
309
309
231
231