Microorganisms of various hazard groups contained on solid media which may also contain hazardous substances,Autoclave controls consisting of,3M Attest vials containing Liqiud media , crushed glass vial and Bacillus stearothermophilus spores and culture,Thermalog S chemical
indicator check strip.
BIOHAZARD.TOXIC.CARCINOGENIC.SENSITIZING AGENTS.3M ATTEST INDICATORS ,TINY GLASS VIALS ARE CONTAINED IN THE PLASTIC OUTER CONTAINER.
As well as the biological hazard from
microoganisms some of the substances incorporated in solid media may be Toxic,carcinogenic and sensitizing agents.
5
INTENDED USE
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr.T.W.Young
Assessment Number*
1103
Date of Assessment
04/01/01
Signature
Assessor
A.Wadeson
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
G6/G7
List names where possible
Dr T,W.Young,A.Wadeson and all other members of Dr T.W.Young's research
team. Final year project students.C.Webster,L Griffiths,R.Godfrey and all personnel who may use the autoclave
for safe discard of the Ground floor communal microbiological waste.
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Sterilisation of microbiological waste,Petri dishes and autoclave controls
using the Labclave 007 autoclave in laboratory G7.
AMOUNT**
Microorganisms of
various hazard groups
contained on solid media
which may also contain
hazardous substances,
Autoclave controls
consisting of,3M Attest
vials containing Liqiud
media , crushed glass vial
and Bacillus
stearothermophilus
spores and
culture,Thermalog S
chemical indicator check
strip.
5
HAZARD
BIOHAZARD.
TOXIC.
CARCINOGENIC.
SENSITIZING
AGENTS.
RISK PHRASES
HAZDAT NO***
BIOSCIENCESNO***
As well as the
biological hazard from
microoganisms some
of the substances
incorporated in solid
media may be
Toxic,carcinogenic
and sensitizing
agents.
3M ATTEST
INDICATORS ,
TINY GLASS
VIALS ARE
CONTAINED IN
THE PLASTIC
OUTER
CONTAINER.
INTENDED USE
Give brief details and attach protocol/instructions
6
Attach copies of data sheet(s)
Sterilisation of waste petri dishes containing microorganisms on solid
media,sterilisation of autoclave controls prior to incubation and after
incubation and sterilisation of control samples of Bacillus
stearothermophilus autoclave controls. Sterilisation af Thermalog S
chemical indicator strips.
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Risk of skin and eye contact with microbiological waste,media components and additions,some of which may be
hazardous.Risk of burns from from hot containers and liquids,risk of bursting of 3M Attest vials and flying bebris
including broken glass. RISK OF CONTACT WITH UNSTERILE MICROBIOLOGICAL WASTE UNTIL
AUTOCLAVE CONTROLS HAVE BEEN VERIFIED.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The risks in section 6 above are significant ,they should be minimised by using Good Microbiological Practice
together with the precautions and instructions given by 3M health care with the Attest vials. The precautions given
in section 8 should be followed.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Face shield should be used to prevent contact with hot waste and flying debris
including broken glass vials. Follow instructions given with attest vials. When the Attest vials are cool and a vial
crusher is not available ,the vial can be wrapped in a cloth and a pair of plyers or forceps used to gently exert
pressure on the vial to break it, prior to incubation. Microbiological waste, which should always be cool enough to
handle safely, should always be handled wearing disposable gloves at all times. Autoclave gloves should be worn
for added safety during glass vial rupture. Ensure adequate ventilation by using the window fan. DO NOT
HANDLE UNSTERILE MICROBIOLOGIACL WASTE OR CONTROLS WITHOUT GLOVES.
Glovesused during the procedure should be put for subsequent sterilisation.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Check integrity of gloves, both disposable and autoclave types.
Check face shield is good order and clean.
10
MONITORING
Performance of control measures,
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Ensure waste agar is washed away with copious amounts of warm water to prevent waste agar solidifying in
drains.Incubated controls, positive, showing growth and negative, showing no growth, should be added to the
waste petri dishes before autoclaving and disposed of in the rest of the plastic mass of waste.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
04/01/06 or
Any change in the protocol.
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Spills of unsterilised microbiological waste should be mopped up with presept granules wearing face shield and
disposable gloves. The same procedure should be used for sterilised waste as the results of the autoclave
controls will not be known until after incubation. Gloves from this procedure should be put for subsequent
sterilisation.
Evacuation, protection for personnel involved in clean-up, Special First Aid
TO PROTECT PERSONNEL
ONLY PERSONNEL WHO ARE ADEQUATELY TRAINED SHOULD ATTEMPT TO CLEAR UP
SPILLAGES.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
After cleaning up spill,wash site with soap and water.
CONTACT
A.Wadeson or First aider.
PHONE
3523 or see
first aider list
10.10.00
*
Prefix T is used for Teaching Assessment Number.
**
List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
***
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.