Molecular Biology EU
Exercise 7/ Seminar 7
Introduction to selected aspects of biotechnology
Polymerase chain reaction (PCR): the general principle of the method, steps applications, types of
PCR: RT-PCR; qRT-PCR; multiplex PCR; nested PCR; RFLP-PCR, etc.
Classical methods of nucleic acids’ sequencing: Sanger sequencing, Maxam–Gilbert sequencing,
semi-automated DNA sequencing.
Next-generation sequencing: pyrosequencing, sequencing by synthesis, ligation and reversible
termination.
Detection of unknown mutations (basic research): RFLP; SSCP; ASO, heteroduplex; DGGE, etc.
Detection of known mutations (diagnostics): ASO, PCR-RFLP; DNA microarray, Southern Blot;
Western blot; Northern Blot
Methods of DNA cloning: restriction enzymes; types of vectors; general rules of vectors selection
DNA cloning applications: medicines, agriculture, industry
Recommended reading:

“Molecular Biology of the Cell”, Alberts B. (e.g. Chapter 8. Manipulating Proteins, DNA, and RNA)
Available online at: http://www.ncbi.nlm.nih.gov/books/NBK21054/?term=genomes)

„Genomes", Brown TA (e.g. Chapter 4. Studying DNA, Chapter 5. Mapping Genomes, Chapter 6,
Sequencing Genomes, Chapter 8. Manipulating Proteins, DNA, and RNA, Chapter 7Understanding a
Genome Sequence).
Available online at: http://www.ncbi.nlm.nih.gov/books/NBK21128/?term=genomes

Campbell Biology. Reece JB (ed.). (e.g. Chapter 20 Biotechnology).

“The Cell: A Molecular Approach”. Cooper GM. (e.g. Chapter 3. Fundamentals of Molecular Biology).

Hernández-Rodrígue P and Gomez Ramirez A. Polymerase Chain Reaction: Types, Utilities and
Limitations
Available online at: http://cdn.intechopen.com/pdfs-wm/37264.pdf

Shendure J and Ji H. Next-generation DNA sequencing. Nature Biotechnology 26, 1135 - 1145
(2008).
Available online at: http://www.nature.com/nbt/journal/v26/n10/full/nbt1486.html

Nollau P and Wagener C. Methods for detection of point mutations: performance and quality
assessment. IFCC Scientific Division, Committee on Molecular Biology Techniques. Clin Chem.
1997 Jul;43(7):1114-28.
Available online at: http://www.clinchem.org/content/43/7/1114.full.pdf+html

http://mol-biol4masters.masters.grkraj.org/Molecular_Biology_Table_Of_Contents.html

http://www.esciencecentral.org/ebooks/applications-of-molecular-genetics/pdf/dna-sequencing-sangerand-nextgeneration-sequencing.pdf

http://www.princeton.edu/~lsilver/book/MG8.html