NATIONAL INSTITUE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING
JHANG ROAD, FAISALABAD:
Tender No. 14/2010
Note
Date of Opening 03-05-2010 at 1430 hours
SINGLE QUOTATION- TWO ENVELOP PROCEDURE:
Chemicals/ kits/Enzymes/Supplies.
Companies should be:1. MP Bio/ICN 2) Qiagen 3) Aldrich 4) Fermentas 5) US Biological 6) GE Health care
7) ABI
8) Cole-Parmer
9) VWR
10) Roth
11) Stratagene 12) Pharmacia 13)
GeneLink,
14)Genomed
15) Bio-Rad
16) BD Biosciences 17) Genesee 18) AG
Scientific 19) Promega 20) Phytotechnology 21) Accurate Chemicals USA 22) B184 23)
Fluka 24) Affinity Bioreagent 25) Roboscreen 26) Bioline UK 27) Amersham 28) Roche
29) Research Organics 30) Merck 31) Macherey-Nagel 32) Sigma 33) Fisher 34) Novagen
35) Calbiochem
Note: Only sole/authorized agents of the above mentioned companies should participate and
certificate must be presented at the time of quote. Cat# must be mentioned with quote for
verification. Import documents must be presented at the time of delivery. MB stands for
Molecular Biology Grade and DNAse/ RNAse free. Plant Cell Culture Tested (PCCT that
means the “Ultra Purity Grade” available to date).
Sole/authorized agents must be registered with any Punjab academic / Research institute.
Specifications of Chemicals/ kits/Enzymes/Supplies
Sr No.
1
2
3
4
5
6
7
8
9
10
11
12
Product
Agar, Total ash ≤6.5%, Gel strength ≥700 Microbiology Grade
Ampicillin, powder, min. 98%, PCCT grade
Bromophenol blue, ACS reagent
Casein enzymatic hydrolysate, PCCT grade
Chloramphenicol, PCCT grade
Chloroform, ≥99.8%, HPLC grade
Chloroform:Isoamyl alcohol 24:1, Ultrapure, MB grade
Ethidium bromide, powder MB grade
Ethanol, anhydrous, 99.5+%, water ≤0.005% MB grade
Ethylenediaminetetraacetic acid disodium salt, (NaEDTA), min. 99%,
MB grade
Ethylenediaminetetraacetic acid iron (III) Sodium salt, crystalline, PCCT
grade
Enzymes
a) Ribonuclease A DNAse free
b) BamH1 Fast Digest within 5-10 minutes
c) BglII Fast Digest within 5-10 minutes
d) ClaI Fast Digest within 5-10 minutes
e) EcoRV Fast Digest within 5-10 minutes
f) EcoRI Fast Digest within 5-10 minutes
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Quantity
1 kg
100g
25 g
1kg
25g
2.5L
500ml
5g
1L
1kg
500g
100mg
1x 800 rect
1x100 rect
1x50 rect
1x200 rect
1x800 rect
Page 1
g) HindIII Fast Digest within 5-10 minutes
h) KpnI Fast Digest within 5-10 minutes
i) NcoI Fast Digest within 5-10 minute
j) NotI Fast Digest within 5-10 minutes
k)PstI Fast Digest within 5-10 minutes
l) SacI Fast Digest within 5-10 minutes
m) SalI Fast Digest within 5-10 minutes
n) SmaI Fast Digest within 5-10 minutes
o)XbaI Fast Digest within 5-10 minutes
p)XhoI Fast Digest within 5-10 minutes
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
q) Taq DNA polymerase
u) T4 DNA Ligase (5 unit/ul)
G418 disulfate salt (antibiotic G418), PCCT
D-Glucose, PCCT
Glycine, PCCT
Hydrochloric acid, 36.5-38% MB grade
myo-Inositol, PCCT grade
Kanamycin sulfate, PCCT grade
Magnesium chloride hexahydrate, ≥ 99%, MB grade
2-Mercaptoethanol, MB Grade, min 98%
Mercuric chloride, min. 99.5%
3-Methyl butano (isoamyl alcohol), MB grade ≥98.5%
Molecular weight markers
a) 1 Kb DNA ladder , ready to use
Plant Growth regulators, PCCT grade
a) 2,4-D
b) 3-indole acetic acid
c) Indole-3-butyric acid
d) 1-Napthaleneacetic acid
e) Gibberellic acid
f ) 6-Benzylaminopurine
g)Kinetin
h) Zeatin Riboside
Phytagel, Plant Cell Culture tested
2-Propanol, >99.5%, ACS reagent
Sucrose, MB grade, min. 99.5%
Tetracycline HCl, Cell culture tested
Plant genomic DNA purification kit
Gel Extraction kit,
PCR Product Cloning kit, 30 reactions
MS salt with Gamborg ( B5) vitamin PCCT
Miniprep plasmid Isolation kit
Plant RNA Isolation kit
Thiamine hydrochloride, plant cell culture tested
1x800 rect
1x300 rect
1x20rect
1x50 react
1x800rect
1x100 rect
1x200 rect
1x100 rect
1x300 rect
10x400 nit
10x500
unit
1x1000unit
2x5g
5x5kg
1x1 kg
1x2.5 L
1x500g
1x25 g
5x1 kg
1x250ml
250 g
1x2.5 lit
1x500appli
1x250 g
1x25 g
25 g
1x100 g
1x5g
1x25g
2x1g
2x50 mg
3x1kg
1x2.5 L
3x5 kg
1x25g
1X100
prep
1X50prep
1x30 rx
1x100 lit
1x250 pre
1x50prep
1x25 g
NOTE:
The soft copy of the financial quotation should also be provided on a W/RW CD
along with your financial quotation please.
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NATIONAL INSTITUE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING
JHANG ROAD, FAISALABAD:
Tender No. 15/2010
Note
Date of Opening 04-05-2010 at 1430 hours
SINGLE QUOTATION- TWO ENVELOP PROCEDURE:
Chemicals/ kits/Enzymes/Supplies.
Companies should be:1.
Plastic ware/Glassware/ Supplies Companies should be
1) Axygen, 2) VWR,
3) USA Scientific, 4) CLP USA, 5) Genesee 6) Greiner, 7) BioRad
8) Fisher 9) SSI 10) Nalgene 11) Schott 12) Pyrex 13) Whattmann 14) MP Bio 15) Bio
Plastic 16) Biolog 17) Roth 18) Brandtech Germany 19) Sorenson Bio 20) Genflow Uk 21)
Nunc 22) Labcon 23) Diagger 24) Parafilm 25) Pipettemann 26) Eppendorf 27) Merck 28)
Denville Scientific
29) Calbiochem 30) Novegen
Note: Only sole/authorized agents of the above mentioned companies should participate and
certificate must be presented at the time of quote. Cat# must be mentioned with quote for
verification. Import documents must be presented at the time of delivery
Sole/authorized agents must be registered with any Punjab academic / Research institute
Specification of Plastic ware/Glassware/ Supplies
Sr.#
1
2
3
4
5
6
7
8
9
10
Product
Conical 50ml pre-sterilized centrifuge tubes , made PP, chemical
resistant, at up to 9000 rpm
PCR Tubes 0.2ml Rnase & Dnase free, autoclave able
Eppendorf tubes 1.5ml graduated Rnase & Dnase free,
autoclaveable
Reagent glass Bottle screw cap 250ml, Temperature stable from -40
t0 +140 , autoclave able
Reagent glass Bottle screw cap 500ml, Temperature stable from -40,
t0 +140 , autoclave able
Reagent glass Bottle screw cap 1 lit, Temperature stable from -40
to +140 , autoclave able
Petri Plates Disposable 90X100mm
Filter paper sheet 10000/pk
Freezing racks 100 holes with attached lid 1.5ml or 2ml tube, Lids
and racks made from autoclaveable PP to handle temperature of -5
to +160 o C
Double sided 81-well racks for 1.ml and 0.5ml tubes, PP made ,
temperature stable upto -80oC, chemical resistant
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Quantity
2x500 nos
5x1000 nos
10000 nos
10
10
10
20 boxes
1 pkt
pkt of 5
pkt of 5
Page 3
11
12
13
14
15
16
17
Tips White 200ul Precise Rnase & Dnase free
Tips White 1000ul Rnase & Dnase free
Flipper Racks with 8 holes for 15 or 50ml tubes , chemical
resistant
Syringe filter 0.22 um , sterile , filtration of additives, tissue culture
media and antibiotics , Hydrophlic memebrane: cellulose mixed
ester
PCR tube racks 168x 0.20 ml , , autoclaveable, PP made
temperature stable -5 to 160 oC
PP 1L Flask with screw caps, autoclaveable
Cryoboxes with 100 holes , made of PP, can be use in freezer to
max -90 oC
10x1000
10x1000
pkt of 5
50/pk
12 no.
20 No.
18
19
20
21
22
23
24
25
NOTE:
2.0ml self or standing cryo vials or micrew tubes , sterile ,
polypropylene made, autoclave able, temperature stable upto -196
o
C , suitable for storing cell culture
PP 1L Beaker with handle , autoclavable
PP 2L Beaker with handle , autoclave able
Parafilm Roll , 4 inchx125ft
1000 ul tip hinged tip refillable box, hold 100 tips, autoclave able
polypropylene
200 ul tip hinged tip refillable box,hold 192 tips autoclave able
polypropylene
Electroporation cuvettes, 1 mm gap, individually w rapped,sterile
Gloves-Latex, large size Powdered
4X 250 no
12 No.
12 No.
25 No
5 No.
5 No.
1x 50 / pk
10x100 no.
The soft copy of the financial quotation should also be provided on a W/RW CD
along with your financial quotation please.
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Page 4