After familiarizing yourself with our policy, screening fees, and data sharing agreement, please submit the following documents along with this completed form by March 1, 2012 to: applicationrnai@nyumc.org
REQUIRED DOCUMENTS
* CV or resume
* Copy of relevant review or publication
* Preliminary results from assay development/optimization
* Optimization budget (see sample budget on p. xxx)
CONTACT INFORMATION
Name:
Email:
Phone:
Fax:
Lab name:
Applicant's position in lab:
Institution department:
SCREENING INFORMATION
Is this a stem cell related project? (Non stem cell related applications are only accepted from the greater NYU research community.)
Yes*
No
*If Yes, please briefly describe how your project is related to stem cell biology?
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Which library and how many replicates would you like to screen? (Screening fees are based on libraries screened in duplicate). If you would like to screen more than one library, a separate application form is required for each screen.
LIBRARY NUMBER
REPLICATES
OF
Human miRNA libraries
Human whole genome
Mouse miRNA libraries
Mouse whole genome
Drosophila whole genome
Drosophila subset (describe below)
Title and description of proposed screen (please describe significance, background and expected hits).
Plate type:
Black clear bottom for visual and fluorescence assays (Corning 3712)
White bottom for luciferase assays (Corning 3570)
Please describe your preliminary results or assay development, including behavior of positive and negative siRNA or dsRNA controls, if available. If you do not have such controls, please explain how you plan to assess your assay robustness or interpret your screening data.
Please describe your secondary screens for ranking potential hits.
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Assay: Human whole genome siRNA screen measuring redistribution of GFP-tagged protein using high content imaging
Optimization budget:
Black, clear bottom plates
Wellmate usage for transfection (6 experiments x 2 hrs)
Wellmate usage for staining (6 experiments x 1 hrs)
$150
$300
$150
Biotek ELX 406 training
Biotek ELX 406
Arrayscan training
(10 experiments x 1.5 hrs)
(3 hours)
$25
$375
$150
Arrayscan usage (10 experiments x 2 hrs)
Consultations with RNAi Core staff (10 hrs)
$800
$500
OPTIMIZATION SUBSIDY REQUESTED=$2450
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