December 2008, Athens

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CURICULUM VITAE
Name:
Work Address:
Rania Anastasiou
Agricultural University of Athens
Laboratory of Dairy Science
Iera Odos 75, 118 55 Athens Greece
+30 210 527 4676
ranastasiou@aua.gr
Tel.:
e-mail:
EDUCATION
1982 - 1987
1988 - 1993
MS in Biology. National and Kapodistrian University of Athens, Greece.
PhD in Biochemistry. National and Kapodistrian University of Athens, Greece.
SCOLARSHIPS
1988 - 1992
1990 - 1991
Institute of Biology. N.R.C. "Democritus" Scholarship for postgraduate studies.
Volkswagen Foundation. Scientific research at the Institute of Botany, University of
Hannover.
RESEARCH EXPERIANCE
1985 – 1986
1990 - 1991
1988 - 1992
1993 – today
Laboratory of Biology. National & Kapodistrian University of Athens, Greece
Institute of Botany. University of Hannover, Germany
Institute of Biology. N.R.C. "Democritus", Athens, Greece
Laboratory of Dairy Research. Department of Food Science and Technology,
Agricultural University of Athens, Greece
SCIENTIFIC ACTIVITY
A. PUBLICATIONS IN REFEREED JOURNALS
1. Anastassiou, R. and Agryroudi-Akoyunoglou J.H. (1995). Thylakoid-bound proteolytic activity vs
exogenous azocoll substrate during chloroplast development in bean. Journal of Plant Physiology
145, 24-30.
2. Bei-Paraskevopoulou, T., Anastassiou, R. and Agryroudi-Akoyunoglou J.H. (1995). Circadian
expression of the Light-Harvesting protein of Photosystem II in etiolated bean leaves following a
single red light pulse: Coordination with the capacity of the plant to form Chlorophyll and the
thylakoid-bound protease. Photosynthesis Research 44, 93-106.
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3. Anastassiou, R. and Agryroudi-Akoyunoglou J.H. (1995). Thylakoid-bound proteolytic activity vs
LHC-II apoprotein in bean. Photosynthesis Research 43, 241-250.
4. Tsakalidou, E., Anastasiou, R., Papadimitriou, C., Manolopoulou, E. and Kalantzopoulos G. (1998).
Purification and characterization of an intracellular X-prolyl-dipeptidyl-aminopeptidase from
Streptococcus thermophilus ACA-DC 4. Journal of Biotechnology 59, 203-211.
5. Tsakalidou, E., Anastasiou, R., Vandenberghe, I., Van Beeumen, J. and Kalantzopoulos G. (1999).
Cell wall bound proteinase of Lactobacillus delbrueckii subsp. lactis ACA-DC 178. Characterization
and specificity towards β-casein. Applied and Environmental Microbiology 65, 2035-2040.
6. Anastasiou, R., Papadelli, M., Georgalaki, M.D., Kalantzopoulos, G. and Tsakalidou E. (2002).
Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl aminopeptidase (PepX) from
Streptococcus thermophilus strain ACA-DC 4. Journal of Applied Microbiology 93, 52-59.
7. Georgalaki, M., Papadelli, M., Anastasiou R., Kalantzopoulos, G. and Tsakalidou E. (2002).
Purification, biochemical characterization and cloning of the intracellular X-prolyl-dipeptidyl
aminopeptidase from Streptococcus macedonicus strain ACA-DC 191. Le Lait 82, 657-671.
8. Anastasiou, R., Leverrier, P., Krestas, I., Rouault, A., Kalantzopoulos, G., Boyaval, P., Tsakalidou E.
and Jan, G. (2006). Changes in protein synthesis during thermal adaptation of Propionibacterium
freudenreichii ssp. shermanii. International Journal of Food Microbiology 108, 301-314.
9. Anastasiou, R., Georgalaki, M., Manolopoulou, M., Kandarakis, I., De Vuyst, L. and Tsakalidou E.
(2007). The performance of Streptococcus macedonicus ACA-DC 198 as starter culture in Kasseri
cheese production. International Dairy Journal 17, 208-217.
10. Papadelli, M., Karsioti, A., Anastasiou, R., Georgalaki, M. and Tsakalidou E. (2007).
Characterization of the gene cluster involved in the biosynthesis of macedocin, the lantibiotic
produced by Streptococcus macedonicus. FEMS Microbiology Letters 272, 75-82.
11. Anastasiou R., A. Aktypis, M. Georgalaki, M. Papadelli, L. De Vuyst and E. Tsakalidou (2009).
Inhibition of Clostridium tyrobutyricum by Streptococcus macedonicus ACA-DC 198 under
conditions simulating Kasseri cheese production. International Dairy Journal 19, 330-335.
12. Georgalaki M., Manolopoulou, E., Anastasiou, R., Papadelli, M. and Tsakalidou E. (2009). Detection
of Streptococcus macedonicus in Greek traditional cheeses. International Dairy Journal 19, 96-99.
13. Georgalaki M., Papadelli, M., Chassioti, E., Anastasiou, R., Aktypis, A., De Vuyst, L., Van
Driessche, G., Devreese, B., Van Beeumen, J. and Tsakalidou E. (2010). Milk protein fragments
induce the biosynthesis of macedocin, the lantibiotic produced by Streptococcus macedonicus ACADC 198. Applied and Environmental Microbiology, 76, 1143-1151.
14. Asteri I-A., Papadimitriou K., Boutou E., Anastasiou R., Pot B., Vorgias C.E. and Tsakalidou E.
(2010). Characterization of pLAC1, a cryptic plasmid isolated from Lactobacillus acidipiscis and
comparative analysis with its related plasmids. International Journal of Food Microbiology,
submitted.
15. Asteri I-A., Boutou E., Anastasiou R., Pot B., Vorgias C.E., Tsakalidou E. and Papadimitriou K.
(2010). Characterization of plasmid pPS1 isolated from Pediococcus pentosaceus: In silico evidence
for the horizontal gene transfer of the σΒ-regulated in Gram-positive bacteria gsiB to lactic acid
bacteria. Applied and Environmental Microbiology, submitted.
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B. PARTICIPATION IN CONFERENCES
INTERNATIONAL CONFERENCES
July 1991, Aghia Pelagia, Crete
NATO ASI Regulation of Chloroplast Biogenesis.
Anastassiou, R. and Argyroudi-Akoyunoglou, J.H.
Thylakoid-bound protease activity as monitored by azo-dye release from azocoll substrate is affected by
thylakoid development and chlorophyll synthesis inhibitors.
July 1991, Aghia Pelagia, Crete
NATO ASI Regulation of Chloroplast Biogenesis.
Agryroudi-Akoyunoglou, J.H., Bei, T., Triantaphyllopoulos, K. and Anastassiou, R. (1991).
Regulation of photosynthetic unit assembly: Regulation of LH apoprotein transcription, translation and
stabilization in thylakoids.
October 1991, Tempe, Arizona
ISPMB Satellite Conference on the Molecular Biology of Photosynthesis. Arizona State University.
Argyroudi-Akoyunoglou, J.H and Anastassiou R.
Thylakoid-bound peptidase of Phaseolus vulgaris.
July 1992, Szeged, Hungary
FESPP Workshop on the Environmental Factors Affecting Photosystem II
Anastassiou, R. and Agryroudi-Akoyunoglou J.H.
Thylakoid-bound protease activity responsible for degradation of LHC-II apoprotein.
September 1992, Nagoya, Japan
IX International Congress of Photosynthesis
Agryroudi-Akoyunoglou, J.H., Anastassiou, R. and Bei-Paraskevopoulou T. Proceedings IX
International Congress of Photosynthesis (Murata N., Ed.) III, 12.327, Kluwer Academic, Dordrecht.
Heat-shock or red-light pulse induced circadian rhythm in the capacity for Chlorophyll synthesis in
etiolated bean and pea leaves. Correlation with PCR and LHC-II apoprotein appearance-stabilization.
September 1995, Kusadasi, Turkey
NATO ASI Workshop. Current Advances in Genetics, Metabolism and Applications of Lactic Acid
Bacteria
Tsakalidou, E., Anastasiou, R., Oikonomou, P. and Kalantzopoulos G.
Cell wall bound proteinase of Lactobacillus delbrueckii subsp. lactis ACA-DC 178. Characterization and
specificity towards β-casein.
June 2001, Ede, The Netherlands
NIZO Dairy Conference On Food Microbes. From knowledge to application
Georgalaki, M., Papadelli, M., Anastasiou, R., Κalantzopoulos, G. and Tsakalidou E.
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Purification, biochemical characterization and cloning of the X-prolyl-dipeptidyl aminopeptidase from
Streptococcus macedonicus strain ACA-DC 191.
June 2001, Ede, The Netherlands
NIZO Dairy Conference On Food Microbes. From knowledge to application
Anastasiou, R., Papadelli, M., Georgalaki, M., Kalantzopoulos, G. and Tsakalidou E.
Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl aminopeptidase (PepX) from
Streptococcus thermophilus strain ACA-DC 4.
September 2002, Egmond aan Zee, The Netherlands
FEMS 7th Symposium on Lactic Acid Bacteria. Genetics, Metabolism and Applications
Anastasiou, R., Papadelli, M., Georgalaki, M., Kalantzopoulos, G. and Tsakalidou E.
Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl aminopeptidase(PepX) from
Streptococcus thermophilus strain ACA-DC 4.
March 2004, Prague, Czech Republic
IDF Symposium on Cheese. Ripening, Characterization and Technology
Anastasiou, R., Kousta, D., Manolopoulou, E., Georgalaki, M., Kandarakis, I. and Tsakalidou E.
Steptococcus macedonicus ACA-DC 198 as adjunct starter in Kasseri cheese production.
June 2004, Saint-Malo, France
International Symposium on Propionibacteria and Bifidobacteria: Dairy and Probiotic Applications
Anastasiou, R., Leverrier, P., Krestas, I., Rouault, A., Kalantzopoulos, G., Boyaval, P., Jan, G. and
Tsakalidou E.
Changes in protein synthesis during thermal adaptation of Propionibacterium freudenreichii ssp.
shermanii strains isolated from traditional Graviera Kritis cheese.
August 2005, Egmond aan Zee, The Netherlands
8th FEMS Symposium on Lactic Acid Bacteria. Genetics, Metabolism, and Applications
Anastasiou, R., Georgalaki, M., Manolopoulou, M., De Malsche, W., Psirras, D., De Vuyst, L. and
Tsakalidou E.
The performance of Streptococcus macedonicus ACA-DC 198 as single and protective starter in Kasseri
cheese production.
August 2005, Egmond aan Zee, The Netherlands
8th FEMS Symposium on Lactic Acid Bacteria. Genetics, Metabolism, and Applications
Papadelli, M., Georgalaki, M.D., Anastasiou, R., Karsioti, A. and Tsakalidou E.
Sequencing of the macedocin biosynthesis regulon, a lantibiotic produced by Streptococcus macedonicus
ACA-DC 198.
June 2006, Nantes, France
1st International Symposium on Antimicrobial Peptides, Food, Veterinary and Medical Applications
Georgalaki, M., Anastasiou, R., Papadelli, M., Devreese, B., Van Driessche, G., Van Beeumen, J. and
Tsakalidou E.
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Induction of Bacteriocin Production in Streptococcus macedonicus ACA-DC 198.
June 2006, Saint Malo, France
American Society for Microbiology. Conference on Streptococcal Genetics
Papadelli M., Karsioti, A., Anastasiou, R., Georgalaki, M. and Tsakalidou E.
Characterization of the gene cluster involved in the biosynthesis of macedocin, the lantibiotic produced
by Streptococcus macedonicus.
October 2007, Goslar, Germany
The 11th International Conference on Culture Collections
Manolopoulou E., Georgalaki, M., Anastasiou, R., Papadelli, M., Aktypis, A., Zoumpopoulou, G.,
Maragoudakis, P., Asteri, I., Papadimitriou, K., Kalantzopoulos, G. and Tsakalidou E.
The ACA-DC Greek Collection of microorganisms.
March 2008, Bern, Switzerland
5th IDF Symposium on Cheese Ripening
De Vuyst L., Leroy, F., Anastasiou, R., Georgalaki, M. and Tsakalidou E.
Streptococcus macedonicus, a multifunctional and promising species for cheese manufacture.
September 2008, Aberdeen, Scotland
21st International ICFMH Symposium (Food Micro 2008) - Evolving Microbial Food Quality and Safety
Papadelli M., Maragkoudakis P., Georgalaki M., Anastasiou R., Zoumpopoulou G. and Tsakalidou E.
Safety evaluation of the macedocin producer Streptococcus macedonicus ACA-DC 198.
GREEK CONFERENCES
December 1992, Athens
35th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
Anastassiou, R. and Agryroudi-Akoyunoglou J.H. Biochemistry and Biophysics Newsletter 35, 92-94.
Thylakoid-bound protease activity: Detection, appearance during thylakoid biogenesis and
characteristics.
December 2001, Athens
53rd Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
Anastasiou, R., Papadelli, M., Georgalaki, M., Kalantzopoulos G. and Tsakalidou E.
Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl-aminopeptidase (PepX) from
Streptococcus thermophilus ACA-DC 4.
April 2005, Athens
1st Hellenic Congress of Food Biotechnology and Food Technology. Oral Presentation.
Anastasiou, R., Georgalaki, M., Manolopoulou, E., De Malsche, W., Psirras, D., De Vuyst, L. and
Tsakalidou E.
Streptococcus macedonicus ACA-DC 198 as adjunct and protective starter in Kasseri cheese production.
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March 2007, Athens
2nd Hellenic Congress of Biotechnology and Food Technology.
Anastasiou, R., Papalexanderatou, Z., van Genderen, F., Wauters, G., Georgalaki, M., Papadelli, M., De
Vuyst, L., and Tsakalidou E.
Inhibition of Clostridium tyrobutyricum LMG 1285T by Streptococcus macedonicus ACA-DC 198.
March 2007, Athens
2nd Hellenic Congress of Biotechnology and Food Technology. Oral Presentation.
Georgalaki, M., Papadelli, M., Anastasiou, R., Vandecasteele, C., Amiradaki, E., Manolopoulou, E. and
Tsakalidou E.
Microbiota of the Greek traditional cheese ‘Arseniko’ from Naxos. Study of physiological properties
related to technology.
December 2007, Athens
59th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
Anastasiou, R., Aktypis, A., Georgalaki, M., Papadelli, M. and Tsakalidou E.
Inhibition of Clostridium tyrobutyricum by Streptococcus macedonicus ACA-DC 198.
December 2007, Athens
59th Meeting of the Hellenic Society of Biochemistry and Molecular Biology.
Georgalaki, M., Papadelli, M., Chassioti, E., Anastasiou, R., Devreese, B., Van Driessche, G., Van
Beeumen, J. and Tsakalidou E.
Induction of Bacteriocin Production in Streptococcus macedonicus ACA-DC 198.
December 2007, Athens
59th Meeting of the Hellenic Society of Biochemistry and Molecular Biology
Papadelli, M., Karsioti, A., Anastasiou, R., Georgalaki, M. and Tsakalidou E.
The biosynthetic gene cluster of macedocin, the lantibiotic produced by Streptococcus macedonicus.
October 2008, Athens
1st Greek Congress on Milk and Dairy Products
Georgalaki, M., Manolopoulou, E., Anastasiou, R., Papadelli, M. and Tsakalidou E.
Detection of Streptococcus macedonicus in Greek traditional cheeses.
December 2008, Athens
1st National MicroBioKosmos (MBK) Conference
Manolopoulou E., M. Georgalaki, R. Anastasiou, M. Papadelli, A. Aktypis, G. Zoumpopoulou, P.
Maragoudakis, I. Asteri, K. Papadimitriou, G. Kalantzopoulos and E. Tsakalidou
The ACA-DC Greek Collection of microorganisms.
December 2008, Athens
1st National MicroBioKosmos (MBK) Conference
Papadelli M., Maragkoudakis P., Georgalaki M., Anastasiou R., Zoumpopoulou G. and Tsakalidou Ε.
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Safety Evaluation of the Macedocin Producer Steptococcus macedonicus ACA-DC 198.
November 2009, Athens
60th Meeting of the Hellenic Society of Biochemistry and Molecular Biology
Asteri I.-A., Papadimitriou K., Boutou E., Anastasiou R., Vorgias K. and E. Tsakalidou
Sequencing and characterization of the novel cryptic plasmid pPS1 carrying a general stress protein
isolated from Pediococcus pentosaceus.
November 2009, Athens
60th Meeting of the Hellenic Society of Biochemistry and Molecular Biology
Papadimitriou K., Anastasiou R., Boutou E., Vorgias K. and E. Tsakalidou
Osmotic stress-resistant mutants of S. macedonicus preserve their ability to produce the bacteriocin
peptide macedocin under hyperosmotic conditions.
OTHER PUBLICATIONS
Anastasiou, R., Georgalaki, M., Manolopoulou, E., De Malsche, W., Psirras, D., De Wuyst, L. and
Tsakalidou E. (2005). The performance of Streptococcus macedonicus ACA-DC 198 as protective starter
culture in Kasseri cheese production. Galaktokomia 16, 66-67.
Georgalaki, M., Amiradaki, E., Tzirita, M., Anastasiou, R., Papadelli, M., Manolopoulou, E. and
Tsakalidou E. (2007). Microflora of the Greek traditional cheese “Arseniko”. Galaktokomia 18, 68-71.
LANGUAGES
October 2010
English, French
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