Appendix 1 Description of the 16 SSR loci used in this study. SSR grouping (1 to 3
according to different PCR conditions, as detailed in Material and Methods), locus
name, range size in base pairs (bp), type of repeat and linkage group (LG) location. The
linkage groups to which markers were assigned are based on genetic linkage map data
of an E. globulus pedigree (unpublished results) and the E. grandis × E. urophylla
consensus map of Brondani et al. (2006).
SSR
Grouping
1
2
Range (bp)
Repeat
LG (consensus)
EMBRA 18
97 - 169
(AG)3GG(AG)19
9
EMBRA 2 b)
124 - 156
(AG)15
11*
EMBRA 6
120 - 156
(AG)19
1
EMBRA 8 a)
133 - 167
(AG)21
6
EMBRA 19
146 - 164
(AG)23
4
EMBRA 23
96 - 152
(AG)16
8
EMCRC 2
151 - 185
(CT)9--(CA)10
11#
EMCRC 5
178 - 252
(CT)19(CA)13
Not mapped
EMCRC 8
229 - 277
(CT)13(CA)24
2
EMCRC 10
314 - 354
(GT)19(GA)9
11
EMBRA 5
96 - 154
(AG)22
5*
EMBRA 11
89 - 149
(AG)4GG(AG)13
1
EMBRA 12
98 - 162
(AG)22
1
EMBRA20
119 - 149
(AG)19
6
EMCRC 7
275 - 305
(TG)15(AG)8
3
EMCRC 11
223 - 259
(TC)10(AC)10
6
Locus
3
a)
dNTPs - 0,1mM each
b)
dNTPs - 0,1mM each and Taq 1U
# putative position, homology was not established
* Mapped in Brondani (2006)
1