dsRNA viruses RNA / protein tables: Edited by Peter Mertens and Denis Bamford
The dsRNA segments and proteins of Bombyx mori cypovirus 1 (BmCPV-1), (strain I) (genus Cypovirus: family Reoviridae):
References [in square brackets]. Recent structural analyses [7, 12, 14]. last updated 13/08/2003
Genome
Segment†
Size
Base
pair
position
of ORFs
(bp)
1
(4190)
2
(3,854)
3
(3,846)
4
(3,262)
[8]
[alternative
nomenclature2]
78-3752
(kDa)
Copy
Number
/
Particle
Protein
Location
Accession
Numbers
VP1
VP2 (Pol)
1225
(136)
unknown
Virion
AF323782
120
virion
AF323781
41-3757
-
[VP3]
1239
(138)
unknown
Virion
AF323783
14-3187
VP3(CaP) [VP4]
1058
(130)
60
virion surface
AF323784
71-2713
6
(1,796)
[4]
44-1726
25-1368
Cognate Proteins‡
Functions and Properties
Orbivirus VP 3(T2)
Orthoreovirus lambda 1
Major virus structural protein forms the capsid shell (CSP) 3
[14]. Comparable to sub-core protein VP3(T2) of
bluetongue virus & Lambda 1 of mammalian orthoreovirus.
Orthoreovirus lambda
3(Pol)
Orbivirus VP 1(Pol)
Rotavirus VP1 (Pol)
-
NS1
[NS5]
881
(101)
0
Non structural
NS2
NS6
[NS5a]
[NS5b]
(80*)
(23*)
0
0
Non structural
Non structural
VP4
[VP6]
561
(64)
unknown
virion
AB035732
NS3
448
(49.8)
0
non structural
NS4
(58*)
0
non structural
(31*)
unknown
virion
390
(44)
unknown
unknown
AB030014
AB030015
-
-
RNA dependent RNA polymerase (Pol),
Polymerase activity significantly stimulated by
S-Adenosyl-l-methionine (Ado Met) or its analogues
Guanylyl transferase, methylases, forms the turret (spike) on
the virion surface. Turret protein (TP)3 [14]..
Amino acid sequence (at position aa 232) contains an
FMDV 2A-like, 'ribosome skip' sequence (NPGP) [13].
This would cause production of some of the protein
molecules (total 881aa) as two polypetides of 234aa and
647aa.
high leucine content (10%) and leucine zipper motif.
ATP/GTP binding protein (virion)
NS3 and NS4 are two non-structural proteins with sizes of
58 and 61 kDa, estimated by SDS PAGE [4].
Large Protrusion Protein (LPP)3 [14].
[VP7]
8
(1,328)
[5]
Protein
Size
aa
1333
(148)
40-4038
5
(2852)
[3]
7
(1,501)
[4]
Protein1
nomenclature
(': Protein
Function)
38-1207
VP5 or P44
[NSP8]
AB016436
If you have any corrections or additional information to add to this webpage please contact Peter Mertens on:
Unknown, shows anomalous migration during PAGE, with
apparent Mr of 55 K.
peter.mertens@bbsrc.ac.uk
dsRNA viruses RNA / protein tables: Edited by Peter Mertens and Denis Bamford
9
(1,186)
[6]
10
(944)
[9, 10]
75-1034
42-797
NS5
[NSP9]
polyhedrin
(Pod)
320
(36)
0
252
(28.5)
unknown
Non structural
AF061199
dsRNA binding [6].
-
polyhedra
D37768
polyhedron matrix protein (Pod) [1, 2, 11].
Size of genome segments and encoded proteins determined by sequence analysis of the genome segments, except where estimated from electrophoretic migration (indicated
by *).
': To facilitate comparisons of proteins across species, genera and families, protein structure / function indicators have been added to protein names : RNA
polymerase = (Pol), Polyhedrin = (Pod). Guanylyl transferase / transmethylase, 'cap' forming enzyme = (CaP). Other species within the genus may have proteins
with significant differences in sizes.
1
: Protein nomenclature based on protein migration order during PAGE (McCrae and Mertens 1983).
2
: [Alternative nomenclature based on the number of the genome segment from which the protein is translated, (Hagiwara et al 2002)].
3
: Structural roles ('capsid shell protein' (CSP), 'turret protein' (TP) and 'large protrusion protein' (LPP)), identified by Zhou et al 2003.
If you have any additional data to add to this table, or any suggested corrections, please mail them to: peter.mertens@bbsrc.ac.uk
Constructed using data supplied by Peter Mertens, Hajime Mori, Hong Zhou and Shujing Rao.
Available as a WebPage (with references) : http://www.iah.bbsrc.ac.uk/dsRNA_virus_proteins/Cypovirus.htm
1.
Reference List
Echeverry, F., Bergeron, J., Kaupp, W., Guertin, C., Arella, M. (1997) Sequence analysis and expression of the polyhedrin gene of
Choristoneura fumiferana cytoplasmic polyhedrosis virus (CfCPV). Gene. 198, 399-406.
2.
Galinski, M. S., Yu, Y., Heminway, B. R., Beaudreau, G. S. (1994) Analysis of the C-polyhedrin genes from different geographical
isolates of a type 5 cytoplasmic polyhedrosis virus. Journal of General Virology 75, 1969-1974.
3.
Hagiwara, K., Kobayashi, J., Tomita, M., Yoshimura, T. (2001) Sequence of genome segment 5 from Bombyx mori cypovirus 1. Archives
of Virology, 146, 181-187.
4.
Hagiwara, K., Matsumoto, T. (2000) Sequences of genome segments 6 and 7 of Bombyx mori cypovirus 1, encoding the viral structural
proteins V4 and V5, respectively. Journal of General Virology 81, 1143-7.
5.
Hagiwara, K., Tomita, M., Kobayashi, J., Miyajima, S. and Yoshimura, T. (1998) Nucleotide sequence of Bombyx mori cytoplasmic
polyhedrosis virus segment 8. Biochemical Biophysical Research Communications 247, 549-553.
If you have any corrections or additional information to add to this webpage please contact Peter Mertens on:
peter.mertens@bbsrc.ac.uk
dsRNA viruses RNA / protein tables: Edited by Peter Mertens and Denis Bamford
6.
Hagiwara, K., Tomita, M., Nakai, K., Kobayashi, J., Miyajima, S. and Yoshimura, T (1998). Determination of the nucleotide sequence of
Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells. Journal of Virology 72, 5762-5768.
7.
Hill, C. L., Booth, T. F., Prasad, B. V. V., Grimes J. M. Mertens P. P. C. Sutton, G. C. and Stuart, D. I. (1999) The structure of a cypovirus
and the functional organisation of dsRNA viruses. Nature Structural Biology 6, 565-568.
8.
Ikeda, K., Nagaoka, S., Winkler, S., Kotani, K., Yagi, H., Nakanishi, K., Miyajima, S., Kobayashi, J. and Mori, H. (2001) Molecular
characterization of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4. Journal of Virology. 75, 988-995.
9.
Ikeda, K., Nakazawa, H., Alain, R., Belloncik, S. and Mori, H. (1998) Characterizations of natural and induced polyhedrin gene mutants of
Bombyx mori cytoplasmic polyhedrosis viruses. Archives of Virology 143, 241-8.
10.
Lavallee, C., Arella, M., Belloncik, S. and Furuichi, Y. (1993) Expression in Escherichia coli of the cloned polyhedrin gene of Bombyx
mori cytoplasmic polyhedrosis virus. Protein Expr Purif. 4, 570-579.
11.
Mori, H., Ito, R., Nakazawa, H., Sumida, M., Matsubara, F. and Minobe, Y. (1993) Expression of Bombyx mori cytoplasmic polyhedrosis
virus polyhedrin in insect cells by using a baculovirus expression vector, and its assembly into polyhedra. Journal of General Virology 74,
99-102.
12.
Tomita, M., Hasegawa, T., Tsukihara, T., Miyajima, S., Nagao, M. and Sato, M. (1999) Two concentric protein shell structure with spikes
of silkworm Bombyxmori cytoplasmic polyhedrosis virus revealed by small-angle neutron scattering using the contrast variation method. J
Biochem (Tokyo) 125, 916-922.
13.
Donnelly, M. L, Luke, G., Mehrotra, A., Li, X., Hughes, L.E., Gani, D., Ryan, M.D. (2001) Analysis of the aphthovirus 2A/2B
polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'. J Gen
Virol.82 :1013-25.
14.
Zhou, Z. H., Zhang, H., Jakana, J., JU, X-Y. and Zhang, J-Q (2003) Cytoplasmic polyhedrosis virus structure at 8Å by electron
cryomicroscopy: structural basis of capsid stability ans mRNA processing regulation. Structure 11 651-663
If you have any corrections or additional information to add to this webpage please contact Peter Mertens on:
peter.mertens@bbsrc.ac.uk