Guimera, N. et al., LR-HPVs in Anogenital Carcinomas
Supplemental digital content
Supplemental digital content 1, Table, Number of invasive cervical, vagina, vulva, penile and anal cancers by patient age, histological categories, HPV genotypes on
whole tissue sections and tumour cells by LCM and p16INK4A staining
Localization
Country
Age
Diagnosis: H&E-1, H&E-2 and H&E-3*
Intake HPV
genotype
HPV genotype/s
on whole tissue
sections from
PCR-1 / PCR-2
p16 INK4A staining
LCM
HPV genotype in
tumor cells
HPV genotype in
non-tumor cells†
% of tumor
cells stained
Pattern
Grade
Cervix
(n=21)
Korea, S
40
Papillary squamous transitional cell carcinoma
6
6 / NA
6
NA
NA
NA
Spain
45
Papillary squamous transitional cell carcinoma
6
6/6
6
0
0
0
Colombia
40
Papillary squamous transitional cell carcinoma
6
6/6
6
26-50
Patchy
2
Spain
Unknown
Papillary squamous transitional cell carcinoma
6
6/6
6
1-25
Patchy
1
France
31
Papillary squamous transitional cell carcinoma
6
6/6
6
0
0
0
Venezuela
50
Papillary squamous transitional cell carcinoma
6
6/6
6
26-50
Patchy
2
Colombia
77
Classical adenocarcinoma
6
6/6
-‡
0
0
0
Korea, S
36
Papillary squamous transitional cell carcinoma
11
11 / NA
11
NA
NA
NA
BosniaHerzegovina
72
Squamous cell carcinoma
42
42 / 42
42
26-50
Patchy
2
Unknown
Squamous cell carcinoma
42
42 / 42
42
>50
Diffuse
3
Italy
78
Squamous cell carcinoma
42
42 / NA
42
NA
NA
NA
BosniaHerzegovina
57
Squamous cell carcinoma
44
44 / -§
-§
0
0
0
Brazil
56
Squamous cell carcinoma
70
70 / NA
70
NA
NA
NA
Philippines
66
Squamous cell carcinoma
70
70 / 70
70
>50
Diffuse
3
Colombia
48
Squamous cell carcinoma
70
70 / 70
70
>50
Diffuse
3
Lebanon
Unknown
Squamous cell carcinoma
70
70 / 70
70
>50
Diffuse
3
Lebanon
54
Squamous cell carcinoma
70
70 / 70
70
>50
Diffuse
3
Mexico
50
Squamous cell carcinoma
70
70 / -§
-‡
>50
Diffuse
3
Italy
75
Squamous cell carcinoma
70
70 / NA
70
NA
NA
NA
Thailand
44
Squamous cell carcinoma
70
70 / NA
-§
NA
NA
NA
Taiwan
41
Adenosquamous carcinoma
70
70 / -§
70
>50
Diffuse
3
Portugal
6
-§
║
-§
Vagina
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Guimera, N. et al., LR-HPVs in Anogenital Carcinomas
(n=1)
Mexico
82
Papillary squamous transitional cell carcinoma
6
6¶ / 6
6
0
0
0
Guatemala
78
Squamous cell carcinoma
6
6/6
6
Colombia
79
Squamous cell carcinoma
6
-§/6
-§
-§
0
0
0
26-50
Patchy
2
Portugal
72
Squamous cell carcinoma
44
44 / 44
-‡
44
0
0
0
Austria
79
Squamous cell carcinoma
44
44 / 44
-§
-§
0
0
0
Austria
79
Warty-Basaloid carcinoma
44
44 / 44
44
0
0
0
Chile
45
Verrucous carcinoma
6
6/6
6
0
0
0
France
48
Verrucous carcinoma
6
6/6
6
1-26
Patchy
1
France
51
Verrucous carcinoma
6
6/6
6
Portugal
70
Basaloid carcinoma
42
42 / 42
42
0
0
0
>50
Diffuse
3
Paraguay
45
Squamous cell carcinoma
6
6 / -§
-‡
0
0
0
Paraguay
53
Warty carcinoma
6
6/6
6
1-26
Patchy
1
Paraguay
74
Warty carcinoma
6
Paraguay
Unknown
Warty carcinoma
6
6/6
6
1-26
Patchy
1
6/6
6
0
0
0
Paraguay
52
Warty carcinoma
6
6/6
6
1-26
Patchy
1
Paraguay
75
Papillary squamous carcinoma
6
6/6
-§
0
0
0
Guatemala
Colombia
40
Warty-Basaloid carcinoma
6
6/6
6
0
0
0
45
Warty carcinoma
6
6/6
6
1-26
Patchy
1
Czech republic
75
Squamous cell carcinoma
6
-§/-§
Excluded
N.D
N.D
N.D
Guatemala
47
Squamous cell carcinoma
11
11 / 11
-‡
-‡
0
0
0
Guatemala
47
Papillary squamous carcinoma
11
11 / 11
-§
-‡
0
0
0
Guatemala
66
Squamous cell carcinoma
11
11 / 11
11
0
0
0
Paraguay
65
Squamous cell carcinoma
11
-§ / -§
Excluded
N.D
N.D
N.D
Paraguay
73
Warty-Verrucous carcinoma
40
6, 40
Excluded
N.D
N.D
N.D
Squamous cell carcinoma
70
70 / -§
70
>50
Diffuse
3
Vulva
(n=5)
Anus
(n=4)
Penis
(n=15)
Mexico
Unknown
†
-‡
-‡
‡
*H&E-3 was only analyzed on cases with block available; In cases where HPV was negative in tumor cells by LCM, other regions were analyzed like: normal epithelium, debris or pre-neoplasic lesions; Human endogenous control gene negative;
§
Human endogenous control gene positive; ║Only tumor cells present ; ¶Only sequencing confirmation on 104bp from L1 ORF; NA, No material available for testing; -, Negative; N.D, Not Done.
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Guimera, N. et al., LR-HPVs in Anogenital Carcinomas
RIS HPV TT/HPV VVAPO study group:
1.
Algeria: Doudja Hammouda (National Institute of Health, Registre des Tumeurs d’Alger), Anissa Bouhadef (C.H.U Hussein-Dey- Hospital Nafissa Hamoud).
2.
Argentina: Silvio Alejandro Tatti, Susana Vighi (Hospital de Clínicas José de San Martín, Universidad de Buenos Aires), Myriam Beatriz Perrota, Ana Jaen, Davis
Kevin (Hospital Italiano de Buenos Aires)
3.
Australia: Suzanne M. Garland, Sepher Tabrizi (The Royal Women’s Hospital, The University of Melbourne), Gerard Vincent Wain, Catherine J Kennedy, Raghwa
Sharma, Yoke-Eng Chiew (Gynaecological Oncology, Westmead Hospital)
4.
Austria: Elmar Armin Joura (University Hospital and Medical School)
5.
Bangladesh: Ashrafun Nessa, AJE Nahar Rahman, Mohammad Kamal (Bangabandhu Sheikh Mujib Medical University – BSMMU), Faruk Ahmed (Dhaka Medical
College Hospital)
6.
Belarus: Halina Viarheichyk, Sitnikov Valeriy (Gomel State Medical University), Achynovich Searhei (Gomel Regional Clinical Oncological Hospital)
7.
Bosnia Herzegovina: Ermina Iljazovic (University Clinical Center Tuzla BiH).
8.
Brazil: Marcus Aurelho de Lima, Sérgio Henrique (Laboratório de Anatomia Patológica e Citologia Ltda, Associação de Combate ao Câncer do Brasil Central,
Hospital Dr. Hélio Angotti, PATMED), Paula Maldonado, Marcia Mannarino, Yara Furtado, Roberto Jose Lima, Gutemberg Leão Almeida, Isabel Val, Renata
Fonseca (Instituto de Ginecologia da Universidad Federal do Rio de Janeiro).
9.
Chile: Rodrigo Prado (Centro de Oncología Preventiva, Facultad de Medicina, Universidad de Chile), Ximena Rodriguez, Marisol Guerrero,Virginia Leiva, Elsa
Olave, Claudia Ramis, Viviana Toro (Complejo Hospitalario San José).
10. China: You Lin Qiao, Chen Wen, Wu Su Hui (Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College).
11. Colombia: Hector Jaime Posso (Liga Contra el Cáncer de Bogotá), Luis Eduardo Bravo, Tito Collazos, Luz Stella García (Hospital Universitario del Valle), Raúl
Murillo, Gustavo Adolfo Hernández Suárez, Carlos Eduardo Pinzón (Instituto Nacional de Cancerología), Gloria I. Sánchez (Universidad de Antioquía).
12. Croatia: Magdalena Grce, Ivan Sabol (Rudjer Boskovic Institutem), Sonja Dzebro, Mara Dominis (Clinical Hospital Merkur).
13. Czech Republic: Ivo Steiner (Faculty of Medicine and Faculty Hospital, Hradec Kralove), Vladimir Vonka (Institute of Hematology and Blood Transfusion), Václav
Mandys (3rd Faculty of Medicine and University Hospital King’s Wineyards)
14. Ecuador: Leopoldo Tinoco (Hospital Oncológico de Quito)
15. France: Christine Clavel, Philippe Birembaut, Veronique Dalstein (CHU Reims, Laboratoire Pol Bouin, Hôpital Maison Blanche), Massimo Tommasino
(International Agency for Research on Cancer), Christine Bergeron (Laboratoire Pasteur-Cerba, Departement de Pathologie)
16. Germany: Monika Hampl, Pof. Baldus (University Hospital of Duesseldorf), Karl Ulrich Petry, Alexander Luyten (Klinikum Wolfsburg)
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Guimera, N. et al., LR-HPVs in Anogenital Carcinomas
17. Greece: Maria Tzardi (Medical School of University of Crete), Theodoros Agorastos (Aristotle University of Thessaloniki).
18. Guatemala: Luis Estuardo Lombardi, Edgar Kestler, Obdulia Salic, Sergio Marroquin, Victor Argueta, Walter Guerra, Sergio Marroquin (Centro de Investigación
Epidemiológica en Salud Sexual y Reproductiva - CIESAR, Hospital General San Juan de Dios), Walter Guerra, Hesler Morales (Instituto Nacional del Cáncer).
19. Honduras: Annabelle Ferrera (Escuela de Microbiología, Universidad Nacional Autónoma de Honduras), Ricardo Bulnes (Hospital General San Felipe).
20. India: Asha Jain (Cancer Prevention and Relief Society Raipur), PSA Sarma (BSP Hospital), Sushil K Giri (Regional Cancer Center); Maheep Bhalla (JLN
Hospital & Research Center), Bharat Patel (Lab One), Neerja Bhatla, Manju Aron, Sandeep Mathur (Institute of Medical Science New Dehli), Ravi Mehrotra
(M.L.N Medical College , Raipur),
21. Italy: Luciano Mariani, Ferdinando Marandino, Amina Vocaturo, Maria Benevolo (Regina Elena Cancer Institute).
22. Israel: Jacob Bornstein, Alejandro Livoff, Hector Itzhac Cohen (Western Galilee Hospital).
23. Japan: Toshiyuki Sasagawa (Kanazawa University Hospital), Shintaro Terahata (Tonami General Hospital), Kazuhisa Ishi (Juntendo University, Urayasu
Hospital).
24. South Korea: Hai-Rim Shin, Jin-Kyoung Oh (National Cancer Center), Jung-II Suh (National Medical Center), Seo-Hee Rha (Dong-A University), Shin Gwang
Kang (Asan Medical Center), Dong-chul Kim (Kangnam St. Mary's Hospital)
25. Kuwait: Waleed Fahad Al-Jassar (Faculty of Medicine, Kuwait University), Rema’a Al-Safi (Maternity Hospital).
26. Lebanon: Muhieddine Seoud (The American University of Beirut Medical Center).
27. México: Patricia Alonso de Ruiz, Gustavo Lastra, Alma Karina Olivares Montano (Hospital General de México, Facultad de Medicina, Universidad Nacional
Autónoma de México), Jorge Salmeron, Isabel Alvarado-Cabrero (Instituto Mexicano del Seguro Social), Rubén López-Revilla, Cuauhtémoc Oros Ovalle, Claudia
Magaña (Instituto Potosino de Investigación Científica y Tecnológica, AC), Ignacio Morones Prieto (Hospital Central, San Luis Potosí).
28. Mozambique: Clara Menéndez, Cleofé Romagosa, Carla Carrillo (Barcelona Center for International Health Research, Hospital Clínic/Universitat de Barcelona,
and Manhiça Health Research Center).
29. New Zealand: Susan M Bigby, RW Jones, KL Fong, D Rowan, J Baranyai, L Eva (Middlemore Hospital)
30. Nigeria: Adekunbiola Banjo, Rose Anorlu, Fatimah B. Abdulkareem, A.O Daramola, CC Anunobi (Lagos University Teaching Hospital Idi-Araba), Sani Malami, Ali
Bala Umar (Faculty of Medicine, Bayero University)
31. Paraguay: Elena Kasamatsu, Francisco Perrota, Antonio Cubilla (Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción).
32. Perú: Carlos Santos, Eduardo Cáceres, Henry Gómez, Juvenal Sánchez, Carlos S. Vallejos, (Instituto Nacional de Enfermedades Neoplásicas).
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Guimera, N. et al., LR-HPVs in Anogenital Carcinomas
33. The Philippines: Efren J. Domingo, María Julieta V. Germar, Jericho Thaddeus P. Luna, Maria Julieta V. Germar, Carolyn Zalameda-Castro, Arnold M.
Fernandez, Roslyn Balacuit (University of the Philippines College of Medicine); Cecilia Ladines Llave, Jean Anne Toral (Cervical Cancer Prevention Center –
CECAP, Cancer Institute (UPCM-PGH)).
34. Poland: Andrzej Marcin Nowakowski (Medical University of Lublin), Robert Jach, Jolanta Orlowska-Heitzman, Monika Kabzinska-Turek, Paulina Przybylska,
Marzena kula-Prykan (Jagiellonian University Medical College)
35. Portugal: Eugenia Cruz (Centro Regional de Oncologia Coimbra, Instituto Português de Oncologia); Manuela Lacerda, Manuel Sobrinho-Simoes (Institute of
Molecular Pathology and Immunology of the University of Porto); Ana Felix, Jorge Manuel Soares (Instituto Portugues de Oncologia de Lisboa Francisco Gentil).
36. Spain: Enrique Lerma (Hospital de la Santa Creu i Sant Pau); Enrique Poblet (Hospital General Universitário de Albacete); Lluís Eleuteri Pons, Patrícia Escrivà
Beltri, Marylene Lejeune (Hospital de Tortosa Verge de La Cinta); Antonio Llombart-Bosch, Morelva Toro de Méndez (Facultad de Medicina, Universidad de
Valencia); Belen Lloveras, Mar Iglesias, Emili Masferrer (Hospital del Mar); Ana Puras Gil (Hospital Virgen del Camino); Miguel Andújar (Complejo Hospitalario
Universitario Insular Marteno-Infantil); Jaume Ordi, Llucia Alòs (CRESIB - Hospital Clínic); Adela Pelayo (Clinica San Carlos); Julio Velasco, Cristina Pérez
(Hospital San Agustín, & IUOPA (Oncologic and Universitary Institute of Principality of Astúrias)); Maria Alejo (Hospital General de l’Hospitalet); Laia Alemany, F.
Xavier Bosch, Vanesa Camón, Xavier Castellsagué, Omar Clavero, Silvia de Sanjosé, Mireia Diaz, Ana Esteban, Jose M. Godínez, Ignacio G. Bravo, Nuria
Guimerà, Yolanda Florencia, Jô Ellen Klaustermeier, Nubia Muñoz, Bea Quirós Cristina Rajo, Sara Tous, Marleny Vergara, (IDIBELL, Institut Català d’Oncologia
(ICO) - Catalan Institute of Oncology); August Vidal (Hospital Universitari de Bellvitge).
37. Taiwan: Chou Cheng-Yang (National Cheng Kung University Medical College, Taiwan Association of Gynecologic Oncologists); Tang-Yuan Chu (Buddhist Tzuchi
Genral Hospital); Kuo-Feng Huang (Chi Mei Medical Center); Cheng Wen-Fang (National Taiwan University Hospital); Chih- Ming HO (Gynecologic Cancer
Center, Cathay General Hospital).
38. Thailand: Saibua C. Bunnag Chichareon, Kobkul Tungsinmunkong, Jintamard Suwanjarat (Prince of Songkla University).
39. The Netherlands: Chris J.L.M Meijer, Peter J.F Snijders (Vrije Universiteit Medical Center); Wim G.V. Quint, Jean-Paul Brunsveld, Anco C. Molijn, Daan T.
Geraets, Jan Lindeman, David Jenkins, Annet Westbroek, René Bax, Linda Struijk, Bernhard Kleter, Maurits de Koning (DDL Diagnostic Laboratory).
40. Turkey: Alp Usubutun (Medical School, Hacettepe University).
41. Uganda: Michael Odida (Uganda Makerere University); Elisabete Weiderpass (Karolinska Institutet, Sweden; The Norwegian Cancer Registry, Norway;
Samfunded Folkhalsan, Finland).
42. UK: Robyn Davies (Manchester Royal Infirmary), Paul Cross (Queen Elizabeth Hospital, Sheriff Hill), Ray Lonsdale (Norfolk & Norwich University Hospital NHS
Foundation Trust)
43. Uruguay: Adela Rosa Sica, Benedicta Caserta, Mabel Cedeira, Daniel Mazal, Guillermo Rodríguez (Laboratorio de Anatomía Patológica del Hospital de la Mujer,
Montevideo)
44. United States of America: Esther Oliva (Massachusetts General Hospital); Thomas C. Wright (New York Presbyterian Hospital, Columbia University Medical
Center), Edyta Pirog (New York Hospital - Cornell Medical Centre), Mark T Goodman, Brenda Y Hernandez (University of Hawaii, Cancer Center, Honolulu,
Hawaii)
45. Venezuela: Enrique López Loyo (Sociedad Venezolana de Patología); Victoria García Barriola, Mirian Naranjo de Gómez, Adayza Figueredo, Janira Navarro
(Universidad Central de Venezuela).
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