Mitochondrial membrane remodelling regulated by a conserved rhomboid
protease
G. Angus McQuibban, Saroj Saurya and Matthew Freeman
Supplementary Information
Figure S1. GFP-tagged Rbd1p retains full activity in vivo. a. A yeast strain in which the
GFP protein was fused to the C-terminus of Rbd1p in the genome by homologous
recombination (RBD1:GFP) displays wild-type growth on media containing glucose and
glycerol. b. The RBD1:GFP yeast strain contains wild-type mitochondria as visualized
by the vital dye Mitotracker Green FM (Molecular Probes).
Figure S2. HA-tagged Mgm1p retains full activity in vivo. a. A yeast strain in which the
HA sequence was fused to the C-terminus of Mgm1p in the genome by homologous
recombination (MGM1:HA) displays wild type growth on media containing glucose and
glycerol. b. The MGM1:HA strain contains wild-type mitochondria as visualized (upper
panel) by the vital dye Mitotracker Green FM (Molecular Probes) and (lower panel)
DAPI staining to see nucleoids. c. Mitochondrial inner membrane targeting of both
untagged Mgm1p and HA-tagged Mgm1p in vivo; both are protected from protease
degradation in the absence of detergent, resembling the protease sensitivity of the inner
membrane protein F1 but not the outer membrane protein Tom40p. Mitochondrialenriched fractions were prepared from late log-phase yeast cultures and subjected to
proteinase K digestion (lanes 1-4, 0, 10, 50, 100 µg/ml; lane 5, 100 µg/ml with 0.1% TX100). Combined with the conserved TMD of Mgm1 (see fig. 3d of main paper), this
implies that full-length Mgm1p resides in the inner mitochondrial membrane.
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