UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
2-Acetylaminofluorene
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☒Hazardous Chemical
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Purpose
2-Acetylaminofluorene is a carcinogen. It is used as a biochemical tool in the study of carcinogenesis to
induce tumors in the liver, bladder, and kidney. Metabolizing this compound in the body is the key to its
carcinogenicity. 2-Acetylaminofluorene is also a substrate for cytochrome P-450 enzyme, which part of a
super family found in almost all organisms.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 53-96-3
Class: Carcinogen
Molecular Formula: C15H13NO
Form (physical state): Solid powder
Color: Beige
Boiling point: 192-196 ºC
Potential Hazards/Toxicity
2-Acetylaminofluorene
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Toxic by inhalation, contact with skin, and if swallowed. Causes genetic damage, as it is a regulated
carcinogen.
Potential Health Effects
Eye: May cause eye irritation.
Skin: May cause skin irritation. Harmful if absorbed through the skin.
Ingestion: Harmful if swallowed. May cause gastrointestinal irritation with nausea, vomiting and diarrhea.
May cause liver damage.
Inhalation: Harmful if inhaled. May cause respiratory tract irritation.
Chronic: Chronic ingestion may cause liver damage. May cause cancer in humans.
Engineering Controls
Always handle 2-Acetylaminofluorene in a certified chemical fume hood. Use dry materials in a fume hood, or
choose premixed solutions to avoid inhalation exposure.
Personal Protective Equipment (PPE)
Respiratory Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Wearing nitrile gloves is recommended.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with 2-Acetylaminofluorene
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses or chemical splash goggles.
Skin and Body Protection
Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be
buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure
while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that
are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hygiene Measures
2-Acetylaminofluorene
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before
reuse. Use only in a well-ventilated area. Minimize dust generation and accumulation. Do not get in eyes, on
skin, or on clothing. Do not ingest or inhale.
First Aid Procedures
If inhaled
Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not breathing, give
artificial respiration. If breathing is difficult, give oxygen.
In case of skin contact
Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing
and shoes.
In case of eye contact
Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower
eyelids. Get medical aid immediately.
If swallowed
If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an
unconscious person. Get medical aid immediately.
Special Handling and Storage Requirements
Precautions for safe handling
All work with 2-Acetylaminofluorene is to be done in an “2-Acetylaminofluorene" designated area in order to
keep 2-Acetylaminofluorene contamination to a minimum. Any persons in this area are required to wear
personal protective equipment. Safety shower and eye wash stations should be easily accessible where 2Acetylaminofluorene is used.
All laboratory equipment (such as beakers, pipettes, etc.) used in the "2-Acetylaminofluorene" designated
area are to be labeled as " 2-Acetylaminofluorene contaminated" and are not to be removed from the area
without first being decontaminated.
Conditions for safe storage
Store in a cool, dry place. Store in a tightly closed container.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
2-Acetylaminofluorene
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
2-Acetylaminofluorene
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Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
2-Acetylaminofluorene
5
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)