Development of Genetic Map in Brassica oleracea using Brassica rapa BAC-SSRs and
Comparison with B. rapa Genome
Wenxing Pang1, Nirala Ramchiary2, Xiaonan Li1, Su Ryun Choi1, Yong Pyo Lim1*
1
2
Molecular Genetics and Genomics Lab, Department of Horticulture, Chungnam National
University, South Korea
Deparment of Biological Science, Institute of Science and Technology, Gauhati University,
Guwahati 781 014, Assam, India
We constructed a genetic linkage map of Brassica oleracea using 188 F2 mapping
population derived from crossing between two diverse cabbage inbred lines, 747 and 748. The
linkage map contains a total of 276 markers and covers a total distance of 1028.8 cM of the B.
oleracea genome; with an average inter locus distance of 3.7 cM. In total, 209 simple
sequence repeat (SSR) which includes markers that anchored to 113 B. rapa bacterial artificial
chromosomes (BACs), 8 intron based polymorphic (IBP) markers and 59 B. oleracea
reference SSR markers were included in the map. The comparative mapping of this newly
developed B. oleracea (C genome) map and reference genetic map of B. rapa (A genome)
showed substantial conservation and re-arrangement between the two genomes. Further, we
identified a total of 22 crucifer building blocks in the 9 linkage groups of B. oleracea. This
map is being used for mapping of morphological traits in B. oleracea.