IACUC LABORATORY SKILLS SHEET
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Name: ______________________________________________
Date: ________________________
Department / Title: ________________________________________________________________________
IMPORTANT: Submit completed form to the IACUC Veterinarian (Biology Dept.); send copy to the Director of Environmental Health & Safety
Use the rating scale below to indicate your level of proficiency on tasks which are capable of performing or have knowledge to
perform in a working environment. Leave blank if you are not capable of performing or lack knowledge to perform the task.
1.
2.
3.
4.
HIGHLY SKILLED – have very recent experience
WELL SKILLED – have performed within two years
SOME SKILL – need training
IN-DEPTH KNOWLEDGE,BUT LACK SKILL TO PERFORM - need training
Animal Care
TISSUE CULTURE
Handling and Restraining:
___Monkeys
___Mice and Rats
___Cats
___Rabbits
___Invertebrates
___Dogs
___Sheep
___Poultry
___Swine
___Feeding
___Cleaning Cages
___Horses
___Dairy Cattle ___Beef Cattle
___Goats
___Breeding
___Assisting in veterinary procedures
___Maintenance and division of continuous lines
___Establish primary cell lines
___Screening for cytoplasma contamination
___Isolation of cell mutants
___Cell fusion
___Cell hybridization
___Staining cells
___Cell cloning
___P3 lab precautions
___Protein and DNA synthesis assays
Animal Surgery
___Preparation for sterile surgery
___Assist in sterile procedure
___Endotrachial intubation and use of ventilators
___Administration of anesthesia: TYPE: ____________
___Venous
___Intraperitoneal
___Subcutaneos
___Tumor implantation into small animals
___Animal Autopsy
___Microsurgery
___Catheterization and fusion of small animal arteries
___Collection of blood
___Post-surgery care
GENETICS
___Mutagenesis
___Mutation tests
___Transduction
___Conjugation
___Maintenance and breeding genetic stock
___Transformation
___Extraction of plasmid DNA
___Isolation of plasmid DNA
___Antibiotic resistance tests
BIOCHEMISTRY
MICROBIOLOGY- BACTERIALOGY
___Maintenance of cultures
___Isolation and purification
Determination and concentration by:
___Direct colony counting ___Antibody titer
___Direct microscope observation
___Infectivity assay
___Inoculation
___Media preparation
___Preparation and assay of bacteriophage
___Plate pouring
MICROBIOLOGY / VIROLOGY
___Fluorescent stains Reverse transcriptase
___ Serological tests
___Hemagglutination inhibition
___Complement fixation ___Neutralization
___RIA
___Virus identification
___Cythopathic effect
___Hemadsorption
___Interferon production
___Other:_______________________________
Cell fractionation:
___Homogenization
___Sonocation
___Differential centrifugation
Purification/ characterization of:
___Enzymes
___Proteins
___Phosphate
___RNA
___Nucleic Acids
___Glycoprotein
___Other:_______________________________________
Gradients:
___Density
___Equilibrium ___Ficoll
___Other:_______________________________________
Assays:
___Enzymes
___Proteins
___RNA
___DNA
___Lipids
___Phosphates ___Receptors binding
___Competitive Binding
___Other:_______________________________________
Electrophoresis:
___Immuno
___Acryl amide gel
___Columns
___Paper
___Agarose
___Labs
___Isoelectric focusing
___2-dimensional gels
Chromatography:
___Paper
___Thin layer
___Affinity
___Column
___High pressure liquid
___Ion exchange ___Gas-liquid
___Immuno-precipitin techniques
In-vitro synthesis of:
___Peptides
___DNA ___RNA
___DNA shearing
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ELECTRON MICROSCOPY
BIOCHEMICAL INSTRUMENTS
___Staining
___Embeding
___Sectioning
___Operation of TEM
___SEM
___Grid preparation
___Measuring molecules
___Shadowing
___Freeze fracture
___Diffusion technique of DNA spread
___Autoanalyzer
___Microburet
___Fluorometer
___pH meter
Spectrophotometers:
___Gamma
___UV
___Visible
___Colorimeter
___Recording
___Atomic absorption
___ESR
___Scintillation counter
___Fraction collectors
___Freeze dryers
___Refractometer
Centrifuges:
___Standard
___High speed
___Model L Preparative Ultracentrifuge
___Model E Analytical Ultracentrifuge
GENERAL LABORATORY TECHNIQUES
___Pipetting
___Weighing
___Sterilizing
___Titration
___Filtering techniques
___Glassware washing
___Autoclave
___Steam and gas
___Sterile technique
___Reagent and buffer preparation
OTHER INSTRUMENTS
IMMUNOLOGY
___Plaque assay for antibody forming cells
___Hemagglutination and hemolysis assay
___Fluorescent antibody technique
___Spectrofluorometry
___Lyophilization
___Microhemagglutination
___Virus isolation
___Antibody titration
___HLA typing
___E-rosette assay
___Plate binding assay
___Making antibodies in animals
___Making monoclonal antibodies
___Incubator
___Darkroom equipment
___Oscillograph
___Volumetric glassware ___Blood gas analyzer
___Hemocytometer
___Plaque counter
___Densitometer
___Micropipetting equipment
___Automatic dispenser ___Particle counter
___Gamma counter
___Microphotographic equipment
ELECTRONICS EQUIPMENT
___Design
___Construction ___Troubleshooting
___Maintenance ___Calibrate
HISTOLOGY
___Perfusion of animal
___Fixation of tissue
Processing and embedding in:
___Paraffin
___Celloidin
___Gelatin
Paraffin sectioning of:
___Biopsy tissue
___Autopsy tissue
___Animal tissue
___Neurologic tissue
___Serial sectioning
___Decalcification
Staining methods:
___Routine hematoxylin and eosin
___Special Stains
___Metallic impregnation
___Histochemical
___Autography of tissue sections
Microtomes:
___Rotary
___Sliding
___Cryostat
DATA PROCESSING
___Systems programming
___Operating computer system
___High level programming on large computers
___Desk top calculators
___Work with Programmer
___Numerical calculations involving chemical stoichiometry
___Statistics
___Database management
COMPUTER LANGUAGES
___FORTRAN
___Assembly
___BASIC
___ALGOL
___Other:_______________________
PHYSIOLOGY
AGRICULTURE
___Preparation of chronic animals
___Stereotaxic implantation of electrodes/cannulae
___Preparation of acute animals
___EEG recording
___EKG recording
Electrode fabrication:
___Intracellular
___Extracellular
___Preparation of brain slices for in vitro recording
___Management
___Marketing
___Crop production
___Field plot research
___Greenhouse
___Soil Science
___Plant Science
___Plant breeding
___Bacterial virus identification
___Test plot design and layout
___Specific anthropod nematode
___Storage Techniques
RADIOBIOLOGICAL
___Bioassays of radioactive isotopes
___Radioimmunoassays of steroids
___Radioisotope handling
___Irradiation of tumors in vitro
___Radioactive labeling
___Other:_________________________________
Important: Submit this completed form to the IACUC
Veterinarian (Biology Dept.) with a copy to the Director
Environmental Health & Safety.
4
IACUC Form-4 (Laboratory Skills Sheet), approved 8dec04
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