Dr. Duan has been working in the field of plant
biotechnology for many years. Currently Dr. Duan is the
molecular team 2 leader in J. R. Simplot company's Plant
Sciences Business, working on research and development
of a number of potato agronomic and consumer traits using
intragenic and cisgenic technology. Dr. Duan obtained his
PhD degree from the University of Connecticut, where he
was a key developer of the Gene-Deletor technology in Dr.
Yi Li's lab. Then he did his postdoctoral research with Dr.
Mary Schuler at the University of Illinois, as a key player
in an NSF 2010 project, functional genomics of plant P450
genes. Dr. Duan has more than 25 publications appearing in
journals such as Plant Physiology, Arch. in Biochemistry
and Biophysics, Plant Biotechnology Journal, PROTEINS,
RNA, Photochemistry Reviews and Transgenic Research.
In this talk he will present his recent work and his point of
view on genetic engineering.
Publications
Li, Y and Duan, H (2011) Molecular approaches for transgene containment and their potential applications
in horticultural crops. In: Transgenic horticultural crops: challenges and opportunities. Mou. B
(ed.) CRC Press (in press)
Chen, Y., Lu, L., Yang, X., Duan, H., Deng, W., McAvoy, R., Zhao, D., Smith, W., Thammina, C., von
Bodman, S. and Li, Y. (2008) Progress report of production of sterile cultivars of euonymus. Acta
Horticulturae. 769: 232-239.
Rupasinghe, S., Duan, H., Frericks-Schmidt, HL., Berthold, DA., Rienstra, CM and Schuler, MA (2007)
High-yield expression and purification of isotopically labeled cytochrome P450 monooxygenases
for solid-state NMR spectroscopy. Biochimica et Biophysica Acta 1768: 3061-3070
Luo, K., Duan, H., Zhao, D., Zheng, X., Deng, W., Chen, Y., McAvoy, R., Jiang, X., Wu, Y., Pei, Y and
Li, Y (2007) A highly efficient system to delete all functional transgenes from pollen of tobacco
plants. Acta Horticulturae. 764: 21-31.
Rupasinghe, S., Duan, H and Schuler, MA (2007) Molecular definitions of fatty acid hydroxylase in
Arabidopsis thaliana. PROTEINS: Structure, Function, and Bioinformatics 68: 279-293
Zheng, X., Deng, W., Luo, K., Duan, H., Chen, Y., McAvoy, R., Song, S., Pei, Y and Li, Y (2007) The
cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activity of
adjacent tissue- and organ-specific gene promoters. Plant Cell Reports. 26: 1195-1203
Luo, KM., Duan, H., Zhao, DG., Zheng X, Deng W, Yongqin Chen, Y., Stewart, CN., McAvoy, R., Jiang,
X., Wu, Y., He, A., Pei, Y and Li, Y (2007) ‘GM-gene-deletor’: fused loxP-FRT recognition
sequences dramatically improve efficiency of FLP or Cre recombinase on transgene excision from
pollen and seed of tobacco plants. Plant Biotech. J. 5: 263-274
Duan, H and Schuler, MA (2006) Heterologous expression and strategies for encapsulation of membranelocalized plant P450s. Phytochem. Reviews 5: 507-523
Schuler, MA., Duan, H., Metin, B and Ali, S (2006) Arabidopsis P450s through the looking glass: a
window on plant biochemistry. Phytochem. Reviews 5: 205-237
Chen, Y., Lu, L., Deng, W., Yang, X., McAvoy, R., Zhao, D., Pei, Y., Luo, K., Duan, H., Y. Li (2006) In
vitro regeneration and Agrobacterium-mediated genetic transformation of Euonymus alatus. Plant
Cell Reports. 25: 1043-1051
Duan, H., Pei, Y., McAvoy, RJ., Zhao, D., Chen, Y., Luo, K., Smith RW and Li, Y(2006): Auxin,
cytokinin and abscisic acid: biosynthetic and catabolic genes and their potential applications in
horticultural crops. J. of Crop Improvement. 18: 347-364
Li, Y., Cheng, Z., Smith, RW., Ellis, D., Chen, Y., Zheng, X., Pei, Y., Luo K., McAvoy, R., Duan, H.,
Thammina, C and Zhao, D. (2006) Gene transfer approaches to neutralize invasiveness of exotic
ornamental plants. J. of Crop Improvement. 17: 279-301
Xiong, A., Yao, QH., Peng, RH., Duan, H., Li, X., Fan, HQ., Cheng, ZM and Li, Y (2006) PCR-based
accurate synthesis of long DNA sequences. Nature Protocols 1: 791-797
Duan, H., Huang, MY., Palacio, K and Schuler, MA (2005) Variations in CYP74B2 (hydroperoxide
lyase) gene expression differentially affect hexenal signaling in the Columbia and
Landsberg ecotypes of Arabidopsis Plant Physiol. 139: 1529-44
Duan, H., and Schuler, MA. (2005) Differential expression and evolution of Arabidopsis
CYP86A subfamily. Plant Physiol. 137: 1067-81.