TERRENCE W BRANN
713 MOTTER AVE
FREDERICK, MD 21701
Phone: 240-344-5226
twbrann@yahoo.com
Abilities
 Sterile technique and bacterial, yeast, and
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tissue culture, isolation of primary cells and
lymphocytes
DNA/RNA extraction & manipulation:
cloning, agarose and SDS/PAGE
electrophoresis, Northern & Southern blots,
PCR, site-directed mutagenesis, gene
sequencing, qPCR, etc.
Use of 96-well plate reader
spectrophotometer and luminometer
Cytotoxicity assays, cell- based viral
neutralization assays
Enzyme linked immunosorbant assay
(ELISA)
Total protein assays, use of visible/UV
spectrophotometer
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Purification of recombinant protein using
FPLC (AKTA)
Western blot, Protein binding assays,
immunoprecipitation,
Neutralizing antibody assays
In vivo studies (mice), tissue harvesting,
processing, and evaluation
Transcriptional regulation analysis by
promoter assay and electrophoretic mobility
shift assay (EMSA)
Adenovirus and lentivirus production
Statistical analysis
GMP lab experience, SOP development
Vector NTI (VNTI) gene analysis (Invitrogen)
Poster, Powerpoint , and oral presentations
MS Office (Word, Excel, PowerPoint )
Employment History
Lab Manager/Research Associate III
07/2003 - 03/2010
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SAIC Frederick Inc, Frederick, MD
Performed research on the effects of cytokines and other agents on the replication of HIV in both primary cells and
established cell lines in a BL2+ laboratory.
Performed research on novel mutations in HIV1 protease and their effect on replication and drug resistance. The results
were published in an article in the Journal of Virology and presented in a poster presentation at the Conference on
Retroviruses and Opportunistic Infections.
Constructed bacterial expression vectors expressing S100A8 and S100A9. Successfully purified S100A8 and S100A9
using AKTA FPLC. Performed experiments showing S100A8/S100A9-induced inhibition of HIV1 production in
macrophages. I used these results in poster and oral presentations at the 2008 International AIDS Conference.
Trained post-doctoral fellows in various techniques, including isolation of monocytes and selection by immuno-affinity
column, and q PCR.
Supervised the maintenance of laboratory equipment and supplies.
Scientist III
02/1996 - 12/2002
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Genetic Therapy, Inc, Gaithersburg, MD
Researched strategies of systemic gene delivery using adenoviral and non- viral vectors.
Constructed plasmids for the generation of adenoviral and lentiviral vectors.
Analyzed tissue and blood samples in a primate study using adenovirus to deliver human clotting factor VIII gene.
Performed neutralization assays with 200 human serum samples to Identify novel adenovirus serotypes for improved gene
delivery.
Successfully produced and tested backbone gene- deleted vectors derived from adenovirus serotypes 5 and 35, which
were used in animal studies.
Developed a novel strategy for protecting adenovirus from neutralizing antibodies using synthetic polymers, which resulted
in a reduction of neutralization by 95% in vitro.
Quality Control Scientist
09/1994 - 02/1996
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ONCOR, Inc, Gaithersburg, MD
Tested research and diagnostic products in a GMP lab for a company that manufactured genebased cancer diagnostic tools.
Performed and helped design and troubleshoot assays for restriction enzymes, DNAase1 and Pol1.
Tested kits manufactured for detection of genes on chromosomes by fluorescent in situ hybridization
(FISH).
Tested kits used for in situ detection of apoptosis in cell sections.
Discovered and implemented corrections to QC testing SOPs that resulted in more consistent and
accurate test results for assays of restriction enzyme lots.
Intern
06/1993 - 08/1993
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Life Technologies, Inc, Gaithersburg, MD
Developed bacterial and mammalian expression vectors for recombinant human neurotrophin 4.
Lab Researcher
04/1991 - 08/1992
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University of Maryland, Baltimore, MD
Studied the effects of cytokines and endotoxin on the viability and permeability of cellular monolayers using radiolabeled
tracers and cytotoxicity assays.
Performed and participated in the design of experiments, maintained tissue cultures.
Supervised radiation safety in our laboratory. Performed computer processing and graphing of data for publication.
Education History
University of Maryland Graduate School
MS Molecular Biology
Boston University
BA Biology / Marine Science