Supplementary Figure legends
Supplementary Figure 1
Cartoon showing PDC tissue with the area used for assessment of
tumor-infiltrating immune/inflammatory cells (A).
In order to extract tumor-infiltrating
immune/inflammatory cells that represented the host immune response against tumor cells, we
selected three areas at low magnification in which the immunolabeled cells had infiltrated the tumor
most densely (A, B), except for the area at the invasive front of the tumor.
We did not select areas
into which infiltrated immune/inflammatory cells had been recruited as a result of secondary tumor
effects, such as pancreatitis, necrosis, ulceration, or mucus flooding of the tissue.
Accordingly, we
did not select areas at the invasive front of the tumor, which is usually affected most severely by
pancreatitis.
The selected areas were those in which it had been confirmed at high magnification
that invasive proliferation of cancer cells was present.
Immunohistochemistry of PDC tissue for
CD8 with nuclear counterstained by Hematoxylin in the low-power view (B) and the high-power
view (C).
Invasive proliferation of PDC cells are found in the areas surrounded by blue lines and
three areas for assessment of tumor-infiltrating immune/inflammatory cells are indicated by the
black square (B).
The black square marked by the asterisk (*) is corresponded to C.
The
majority of immune/inflammatory cells were attached to tumor cells in the area shown (C).
Supplementary Figure 2
Kaplan-Meier survival curves showing comparison of disease-free
survival between high (orange) and low (blue) infiltration of immune/inflammatory cells.
P
values obtained from log-rank test. The "x" and "+" represent censoring and failure, respectively.
The proportions of 5-year survival with 95% confidence intervals (in parentheses) are shown.
*Ratio of CD68+HLA-DR+ M1 macrophages to CD68+ pan-macrophages, **ratio of FOXP3+CD4+
Tregs to CD4+ T cells.
Supplementary Figure 3
(Left) Venn diagram defining relationships and subpopulations among
populations of patients with CD4+Thigh, CD8+Thigh, or %Treglow. (Right) Kaplan-Meier survival
curves showing comparison of overall survivals for the subpopulations defined in the Venn
diagram.