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Figure S1 Exemplary bacterial automated intergenic spacer analysis (ARISA) profiles
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obtained for gut walls of common earthworms and soils sampled from a wheat-barley field
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(Johnstown Castle). ARISA profiles were obtained using DNA extracted from gut walls of
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Aporrectodea longa (A), A. caliginosa (B), Lumbricus terrestris (C) and soil (0-20 cm depth)
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collected from the earthworm sampling spots (D). All samples originated from the same
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wheat-barley field. ARISA was conducted using the bacterial primer set 6-FAM tagged
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ITSF/ITSReub (Cardinale et al., 2004) and products were analysed by capillary
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electrophoresis with fluorescent laser detection.
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Figure S2 Exemplary bacterial automated intergenic spacer analysis (ARISA) profiles
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obtained for gut walls of common earthworms and soils sampled from a grass-clover field
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(Johnstown Castle). ARISA profiles were obtained using DNA extracted from gut walls of
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Aporrectodea longa (A), A. caliginosa (B), Lumbricus friendi (C) and soil (0-20 cm depth)
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collected from the earthworm sampling spots (D). ARISA was conducted using the bacterial
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primer set 6-FAM tagged ITSF/ITSReub (Cardinale et al., 2004) and products were analysed
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by capillary electrophoresis with fluorescent laser detection.
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Figure S3 Non-denaturing polyacrylamide gel (4% w v-1) electrophoresis profiles of partial
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16S and 23S rRNA genes and intergenic spacer region defined bacterial community tightly
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associated with gut walls of different earthworm species and soils collected from a wheat-
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barley field (lanes: 1 to 5) and a grass-clover field (Lanes: 6 to 10) (Johnstown Castle Estate).
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Lanes: 1, 2, Lumbricus terrestris; 3, 9, A. caliginosa; 4,10, A. longa; 5,6, soils (0-20 cm
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depth); 7,8, L. friendi; L, 100 bp DNA marker (Invitrogen); B, blank PCR with loading dye.
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The RISA bands marked by arrow pointers were excised for cloning and sequencing (See SI
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Text).