OHS026
Safe Work Procedure
Faculty/Division
Medicine
Document number
SOMS.CGM.SWP.019
School/ Divisional Unit
School of Medical Sciences
Initial Issue date
26 June 2009
Current version
1
Current Version
Issue date
26 June 2009
Next review date
26 June 2012
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of this
form.
Safe Work Procedure Title and basic description
Title: Handling cytotoxic compounds: the anti-tropomyosin compound series
Description:
Associated risk assessment title and location: 19_RA_Handling cytotoxic compounds_TB
Describe the activity or process
IMPORTANT: The anti-tropomyosin compound series are of unknown toxicity and therefore must be handled
with extreme caution. The experimental operator must wear personal protective equipment (lab gown, safety goggles,
mask, gloves (double gloving recommended)) when handling these compounds.
These compounds are known to have an anti-proliferative effect in cancer cell lines and are therefore classed as
cytotoxic.
Preparing compounds
1.
2.
3.
To make an anti-tropomyosin compound stock solution, take a pre-weighed tube from the desiccator and fill in
the log sheet (tube #, date of removal and reconstitution method). In the situation that the compound is not preweighed, a mask must be worn when weighing the compound.
In a biological safety cabinet reconstitute the compound to 50mM in sterile DMSO. Use a DMSO ampule which is
free from moisture.
Store the stock solutions either in a desiccator at room temperature or at -80°C.
Using compounds for cell culture assays*
1.
2.
To perform a cytotoxicity assay with these compounds in a mammalian cell line, serially dilute the compounds in
the appropriate cell culture media (i.e. 8 concentrations in the log range).
Using a multi-channel pipette add the compound/media solution to cells following a 24hr incubation period.
* See SWP ‘AlamarBlue assay’ or ‘MTT assay’ for performing a cytotoxicity assay.
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc
Reagents and equipment:

Anti-tropomyosin compound series

DMSO (Dimethyl sulfoxide)
Personal Protective Clothing

Lab gown

Gloves

Safety goggles

Mask
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Page 1 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007
List potential hazards and risk controls including
specific precautions required
.

The anti-tropomyosin compound series are of unknown toxicity and therefore must be handled with extreme
caution. The experimental operator must wear personal protective equipment (lab gown, safety goggles, mask,
gloves (double gloving recommended)) when handling these compounds.
Eye contact: immediately flush eyes with running water for 15 minutes. SEEK MEDICAL ADVICE
Skin contact: wash with soap and water. SEEK MEDICAL ADVICE
Ingestion: do NOT induce vomiting unless directed to do so by medical personnel

DMSO is slightly hazardous in case of inhalation (lung irritant), skin contact, eye contact and ingestion. Use
protective gloves, safety glasses and lab gown when handling DMSO.
Eye contact: immediately flush eyes with running water for 15 minutes.
Skin contact: wash with soap and water.
Inhalation: if inhaled, remove to fresh air. If not breathing, give artificial respiration
Ingestion: do NOT induce vomiting unless directed to do so by medical personnel
List emergency shutdown instructions

Chemical spill: In the case of a chemical spill notify a person immediately and access the chemical spills kit
located in Rm502.
List clean up and waste disposal requirements




Ethanol and wipe-down all used surfaces.
All disposables in contact with the anti-tropomyosin compounds (i.e. pipette tips, pipettes, plates, eppendorfs,
kimwipes, etc.) must be disposed of in cytotoxic waste containment.
Plates containing anti-tropomyosin compounds should be tightly bound in parafilm and bagged before disposal
in cytotoxic waste containment.
PC2 Safe Work Practices apply. Wash hands before leaving the PC2 facility.
List legislation, standards and codes of practice used
in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Australia Dangerous Goods Code
Code of Practice for the Labelling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
AS/NZS 1336:1997 Recommended Practices for Occupational Eye Protection
UNSW Laboratory Hazardous Waste Disposal Procedure (OHS321)
Safe Work Procedure Form (OHS026)
Supervisory approval, training, and review
Supervisor: Peter Gunning
Signature:
Plant custodian:
Signature
List competency required – qualifications, certificates, licencing, training - eg course or instruction:
Training as per Training Needs Analysis, Induction to Lab, Training on this SWP
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Page 2 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007