Curriculum Vitae
Personal Information
First Name:
Last Name:
Date of Birth:
Contact Number:
Dzmitry
Mukha
27/01/1986
+375 29 5629979
Gender:
male
Nationality:
Belarus
Email:
d.v.mukha@gmail.com
Contact Address: Russiyanov Street, 50 - 405, 220141, Minsk, Belarus
Professional Profile
Current Position:
Current Research:
Affiliated Institution:
Research Fellow
Current research is devoted to developing pipelines for in silico and in vitro screening of new potential
drug compounds. The work comprises creation of genetic constructions, expression, and
chromatographic purification of recombinant proteins from different sources including humans.
Computational biology tasks include construction of precise spatial models of protein using
sophisticated molecular dynamics technics and performing screening procedures based on both
numerical descriptors and spatial properties of chemical compounds. Few original algorithms of
protein properties modeling were developed. Results of computational procedures are validated by
different spectroscopy experiments with recombinant proteins obtained. The main focus of current
studies is investigation of human cytochromes performing xenobiotics conversion.
Tauber Bioinformatics Research Center, Haifa University
Education
2012/09
2010/10
2008/11 – 2011/11
2008/06
Sofia School of Protein Science: Structure and dynamics of biological macromolecules, FEBS Lecture
Course, Sofia, Bulgaria
BioCrys2010, FEBS Practical and Lecture Course, Fundamentals of Modern Methods in
BioCrystallography, Oeiras, Portugal
PhD student in Biochemistry, Institute of Bioorganic Chemistry, NAS of Belarus
Graduated from Department of Genetics, Biological Faculty of Belarusian State University, Minsk,
Republic of Belarus (‘Heterological expression of human cytochrome P450 3A4 and its physicalchemical characterization’)
Employment history
2012/06 – present
2011/11 – present
2008/08 – 2011/11
Consultant, Tauber Bioinformatics Research Center, Haifa University
Research Fellow, Laboratory of Protein Engineering, Institute of Bioorganic Chemistry, National
Academy of Sciences, Minsk, Republic of Belarus.
Junior Research Fellow, Laboratory of Protein Engineering, Institute of Bioorganic Chemistry, National
Academy of Sciences, Minsk, Republic of Belarus.
Academic Awards
2010/12
2011/01
Gratitude from President of National Academy of Sciences
‘Best first publication’ prize of National Academy of Sciences
Professional Qualifications
Computer skills:
Experimental methods:
Languages:
Experienced in using/administration of rpm-based Linux (Fedora 12 -17, Red Hat v.5.x, v.6.x, CentOS
5.x, 6.x), programming on Java, bash scripting, advanced user of PBS/Torque cluster management
middleware, GRID Unicore middleware, UCSF AMBER 10/11/12, GAMESS, UCSF Dock v.6.5, user of
MOSFLM, CCP4.
Electron spectroscopy, cloning, recombinant protein expression, protein chromatographic purification,
biochemical assays, mass spectroscopy.
English, Russian, Belarusian.
Selected publications
Articles:
 Ershov P, Mezentsev Y, Gnedenko O, Mukha D, Yantsevich A, Britikov V, Kaluzhskiy L, Yablokov E, Molnar A, Ivanov A,
Lisitsa A, Gilep A, Usanov S, Archakov A. Protein interactomics based on direct molecular fishing on paramagnetic
particles: Experimental simulation and SPR validation. Proteomics. 2012 Sep 24. doi: 10.1002/pmic.201200135. [Epub
ahead of print]
 Mukha D. V., Feranchuk S. I., Gilep A. A., Usanov S. A. Molecular Modeling of Human Lanosterol 14α-Demethylase
Complexes with Substrates and Their Derivatives. Biochemistry (Moscow), 2011, V. 76, I. 2, pp. 175-85.
Conference abstracts:
 Mukha, D. V., S. A. Usanov. A novel method of protein-solvent binding energy evaluation via analysis of molecular
dynamics trajectories. The 4th International IMBG Conference for Young Scientists «Molecular biology: advances and



perspectives», Kyiv, Ukraine, September 14-17, 2011. — P. 199.
Mukha, D. V., Usanov, S. A. Elucidation of the functional role of amino acids forming the substrate-binding site of
human CYP51A1 by molecular modeling and sequence analysis. 17th International Conference on Cytochrome P450,
Manchester, UK, 26th-30th of June 2011. – Manchester, UK, 2011. – P. 23.
Gilep A. A., Shpak S. V., Dvornikov A. C., Mukha D. V., Usanov S. A. Insights into the specificity of vitamin D binding
proteins. 17th International Conference on Cytochrome P450, Manchester, UK, 26th-30th of June 2011. – Manchester,
UK, 2011. – P. 74.
Strushkevich N. V., Mukha D. V., Gilep A. A., Park H.-W., Usanov S. A. Structural basis of human CYP51 inhibition by
antifungal azoles. 45th Meeting of the Polish Biochemical Society, Wisla, Poland, September 20 – 23, 2010, Slaski
Medical University in Katowichi, p. 55.