Evaluated
for NGS*
DANAGENE SALIVA KIT
Kit designed for an efficient and fast purification
of highly pure genomic DNA from a wide variety
of saliva samples including:
1. Saliva samples.
2. Buccal cell samples via mouth swabs from
DANASALIVA Swabs. Ref.0602.42
3. Saliva samples via DANASALIVA
Sample Collection Kit. Ref.0603.43
4. Saliva samples via ORAGENE self
collection kits (DNAGenotek).
Procedure: The process includes a cell lysis with an
anionic detergent that solubilizes the necessary cell
components, the contaminant RNA can be removed
with a RNase treatment. The cell proteins are removed
by precipitation, which allows to leave the genomic DNA
in solution. Finally, the genomic DNA is isolated by
a precipitation with isopropanol.
Applications: DNA purified using DANAGENE
SALIVA KIT is highly stable and suited for use in a
wide range of applications such as:
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
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DNA archiving.
PCR and quantitative real-time PCR.
SNP analysis.
Southern Blotting.
Next Generation Sequencing.
*Validation of the DANAPURE SALIVA KIT on
the ROCHE 454 TITANIUM Next-Generation
Sequencing Platform was realized in the Centro
de Investigación, Tecnología e Innovación (CITIUS)
Universidad de Sevilla.
Quantification. Using the Quant-iT Picogreen
dsDNA Reagent from INVIROGEN.
Library Quality Assessment. Run a 1 l aliquot of
the DNA library on an Agilent Bioanalyzer High
Sesitivity DNA chip, to assess the quality of the
library.
Cell lysis
Protein DNA
Precipitation
DNA Wash
DNA
Hydration
Features:
 DNA from saliva is equivalent to DNA from
blood for downstream applications.
 Improve patient care and compliance with
painless, non-invasive sample collection and
decreases costs.

Library Quantitation for qPCR. KAPA Library
Quantification Kit from Kapabiosystems.
The DNA isolated with the DANAPURE SALIVA
KIT from different samples of saliva has an
excellent quality to be used in NGS:
 double-stranded.
 OD260/280> 1.75
 concentration > 10 ng/l.
 Library Quality: Fragment size between
1400 and 1800 bp.
 Library Quantitation: > 1 x 107 molecules/l.
Reproducible, fast and non-expensive
method.
 Safe method, as it removes completely the
need of using toxic reagents.
DANAGENE SALIVA KIT
Ref.0603.4 / 06043.41
RNase and Proteinase K included.