Data Entry Sheet and Checklist
Urban Microbial Community Dynamics and Metagenomics
Data Entry Sheet and Checklist
Student name(s):
1. _______________________________
2. _______________________________
3. _______________________________
4. _______________________________
Lab section: __________
Meeting time: __________
Instructor: _____________
Semester/Year: ________
Step 1(sample collection):
Sample code number: _____________
_________________
Sample collection location:
Sample collection date: ___________
Notes on sample collection:
Step 2 (PowerSoil genomic DNA extraction and purification):
Approximate volume of sample (step 1e from protocol): __________
Approximate volume of sample added to each of the PowerBead tubes (step 1f1): ______
Volume of elution in last step of PowerSoil DNA purification: _________
Tube label identifier for your sample: _____________________
This tube will be stored at -20C by your instructor.
Step 3 (DNA quantification by NanoDrop):
Sample ID: ________________
DNA concentration (ng/ul): ____________ Date:
___
260/280 ratio*:____________
260:230 ratio**:____________
* A 260/280 ratio of ~1.8 is generally accepted as “pure” for DNA and ** a 260:230 ratio
of 1.8 – 2.2 is accepted as “pure” for DNA.
Step 4 (PCR amplification):
Label on small PCR tubes:___________
used:___________
Reaction volume for PCR:________
(ul)?______
Forward primer (bar coded)
How much template DNA was used
Step 5 (Gel analysis and quantification):
Lanes used on the gel: _________
sample?______
How many ul were loaded of your
1
Data Entry Sheet and Checklist
How many bands were detected?____
What size?______
Approximate quantification of PCR product based on gel analysis:_______
Notes on the gel:
Step 6 (PCR clean-up and submission of sample for sequencing):
Volume DNA eluted in:_____
Concentration of the DNA (ng/ul):____
2