RECOMBINANT INBRED LINES (RILs)
Hawaii Foundation Seeds
1. SUMMARY.
More than 1200 RILs (recombinant inbred lines) are available from HFS. They were created by
selfing from singlecrosses involving elite maize inbreds (largely by Moon, H.G. et al., 1999,
Maydica 44:301-311). The parents are described fully in Section 4 below. They included
temperate inbreds B73 (Iowa), DB544 (Korea), Ia453sh2 (Iowa), Fla2BT73 (Florida), Hi31
(Hawaii, ex Iowa B68) and Mo17 (Missouri). Tropical inbreds included Hi34 (Hawaii, ex
Antigua), Hi38bt (Hawaii), Ki14 (Thailand), Narino 330 (Philippines), Tx601 (Texas, from
Tuxpeno), TZi4 (Nigeria, ex Guatemala) and TZi17 (Nigeria). All lines are publicly available
from Hawaii Foundation Seeds; most have been converted to Mv gene for maize mosaic
resistance.
2. RILs created at HFS, with major segregating QTLs
A. Hi34 x Tx601. 115 rils. Head smut (HEAD), Fall armyworm (FAW), Ostrinia stalk borers
(OST), plant height (PH), ear height (EH), kernel weight (KW)
B. B73 x Narino 330. 100 rils. Maize dwarf mosaic virus (MDMV), Maize mosaic virus (MMV),
downy mildew (DM), southern rust (POLY), Northern blight (TURC), OST, dentiness
(DENT) maturity (DTS), PH, daylength sensitivity (DAYS), EH, internode length (INT),
tassel branching
C. B73 x Tx601 (103) MDMV, DM, kernel rot (FUS), FAW, EH, leaf number (LNO), INT,
DTS, POLY
D. B73 x TZi4 (118) DM, Maize streak virus (MSV), POLY, common rust (SORG), TURC,
FAW, OST, DTS, Seed color (WHITE), DAYS, LNO, INT, Striga witchweed
E. DB544 x Fla2BT73 (118) Black streak dwarf virus (BSDV), Bacterial leaf blight (BLB),
Southern blight (MAYD), STRIGA, DTS, PH, EH, LNO, tip leaf burn, tassel branches
G. Hi31 x Ki14 (250) MDMV, MMV, DM, Maize stunt mycoplasm (STUNT), Lime-induced
chlorosis (LIME), Curvularia maydis, POLY, Stewart’s wilt (WILT), MAYDIS, Ustilago
maydis (SMUT), TURC, DS, pericarp thickness (PT), staygreen (SG), tassel type, KW
H. Hi34 x TZi4 (142) MMV, MSV, DM, SORG, root lodging (LODG), WHITE, INT
I. Hi34 x TZi17 (109) MMV, MSV, STUNT, SMUT, SORG, TURC, Stalk rot, OST,
STRIGA, WHITE, tassel length, KW
J. Tx601 x TZi4 (121) MMV, SMUT, SORG, TURC, OST, PH, Kernel Color
L. Mo17 x Nariño 330 (145) MMV, DM, DENT, MAT, POLY
M. Ia453sh2 x Hi38bt (70) MMV, MAT, SORG, POLY, DAYS, Flag leaves (FLAG),
Tillering (TIL), sh2 and bt loci
3. MOLECULAR MAPPING
Set G (127 RILs) was RFLP-mapped by Ming et al. (1999, Maydica 44:319-323), working
with Mike McMullen (U. Mo.). Set H was partially mapped at OARDC (Ohio) by Kyetere et al.
(1999 Genome 42:20-26). Set I has been mapped at CIMMYT by X. Lu and S. Nourse (Lu et al.,
Maydica 44:313-318).
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4.
DESCRIPTION OF PARENT INBREDS
Inbred
B73
DB544
Fla2BT73
Hi31
Hi34
Hi38c
Ia453sh2
Ki14
Mo17
Narino330
Tx601
TZi4
TZi17
Type
Te Y D
Te Y F
Te Y D
Te Y D
Tr Y F
Tr Y bt
Te Y sh2
Tr Y F
Te Y D
Tr Y F
Tr Y D
Tr W F
Tr W F
Origin
Iowa
Korea
Florida
Hawaii
Hawaii
Hawaii
Iowa
Thailand
Missouri
Thailand
Texas
Nigeria
Nigeria
Description
Iowa Stiff Stalk Synthetic
Korean Composite
Florida Multiple Cross
B68 (Iowa) = (Argentine Flint x B14)BC3
Antigua 2D
Hi bt Comp 3 (tropical x temperate)
Ia453su1 (conversion to sh2)
KU1414, from Suwan 1 Composite
187-2 x C103
Cuban Flint Variety
Yellow Tuxpan
IITA1393 Guanacaste7729xTZSR
IITA9091, Oh43 x Rpp Streak Resistant Comp.
Te = temperate, Tr = tropical; Y = yellow, W = white; D = dent, F = flint; Several inbreds above
were given Hi designations after conversion to Mv gene for resistance to Maize Mosaic Virus,
including: B73 (Hi47), Ki14 (Hi58), Mo17 (Hi60), Tx601 (Hi65) and TZi4 (Hi66).
References:
Brewbaker, JL and AD Josue. 2007. Registration of 27 maize parental inbred lines resistant to maize mosaic virus.
Crop Sci. 43:459-461.
Moon, HG, JL Brewbaker and XW Lu. 1999. Major QTLs for disease and insect resistance identified among
recombinant inbred lines from tropical maize hybrids. Maydica 44:301- 311.
QTLs from RILs for NORTHERN LEAF BLIGHT
30
Observed
FREQUENCY
25
20
15
Expected
10
5
0
1
2
3
4
5
6
7
8
9
Blight scores (1 = Resistant)
Example of use of RILs to identify QTLs (genes) affecting a trait, in this example the tolerance
of Southern Rust (Puccinia polysora) in Set G RILs where a single major QTL is “expected” and
confirmed. (from Moon et al., 1999).
(docs:\2015 web\rils.doc)
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