Related to deliverable No: D.3.4.1, 3.4.5
Version 20 Nov. 09
Tool title and potential use
Stress protein measurement in Danio rerio larvae to investigate the proteotoxicity
of chemical mixtures
Novelty and background
Stress proteins (especially Hsp70) have been used for several years to determine the
proteotoxicity of enviromental pollutants. Within the NoMiracle project, a test on
Hsp70 stress protein reaction in zebrafish (Danio rerio) larvae was developed and
adjusted to be conducted using single chemicals as well as mixtures of chemicals as
stressors.
Description of tool and current state
The tool is ready for use. Zebrafish embryos and larvae are raised in the respective
test concentrations in Petri dishes. At the age of 168h post fertilisation, the Hsp70
content of 8 pooled larvae is measured. Results of tests were published in [1, 2]
References
1. Scheil V, Kienle C, Osterauer R, Gerhardt A, Köhler H-R (2009) Effects of
3,4-dichloroaniline and diazinon on different biological organisation levels
of zebrafish (Danio rerio) embryos and larvae. Ecotoxicology 18: 355-363.
2. Scheil V, Zürn A, Triebskorn R, Köhler H-R (2009): Embryo development,
stress protein (Hsp70) responses and histopathology in zebrafish (Danio
rerio) following exposure to nickel chloride, chlorpyrifos and binary
mixtures of them. Environ Toxicol.
Responsible scientists:
Heinz-R. Köhler, University of Tübingen, heinz-r.koehler@uni-tuebingen.de
Volker Scheil, University of Tübingen, volker.scheil@uni-tuebingen.de