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Supplemental figure 1
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Schematic representation of the principle of transgene mapping. Solid bars indicate
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chromosome pair which contains the exogenous DMY in the individuals. Blue horizontal
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lines indicate the position of the DNA marker on the chromosome. The concordance rate is
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expected to be 100% in the locus adjacent to the exogenous DMY.
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Supplemental figure 2
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Determination of real-time PCR efficiencies of target gene (DMY) and reference genes
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(Elf1a and β-Actin) for quantification of the copy number (a) and the expression levels (b).
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Threshold cycles and volume of the template were plotted to calculate the slope (mean
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value; n=3). The corresponding real-time PCR efficiencies were calculated according to the
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equation: E=10[-1/slope] (Rfaffl, 2001). Under the condition that Etarget = Ereference = 2, the
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comparative Ct method is applicable for a quick estimation of the relative ratio
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2001).
(Pfaffl,
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Supplemental figure 3
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Light micrographs of adult testes in Hd-rR inbred strain (a), DMY transgenic strain 1 (b) and
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2 (c). ed, efferent duct; Scale bars, 100μm
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