SUPPORTING INFORMATION
Facet-Specific Assembly of Proteins on
SrTiO3 Polyhedral Nanocrystals
Lingqing Dong1,2,†, Qi Luo3,†, Kui Cheng1,†, Hui Shi3, Qi Wang3, Wenjian
Weng1,4,
1
★
& Wei-Qiang Han2,
★
Department of Materials Science and Engineering, State Key
Laboratory of Silicon Materials, Cyrus Tang Center for Sensor Materials
and Applications, Zhejiang University, Hangzhou 310027, China.
2
Ningbo Institute of Materials Technology & Engineering, Chinese
Academy of Sciences, Ningbo 315210, China.
3
Soft Matter Research Center and Department of Chemistry, Zhejiang
University, Hangzhou 310027, China.
4
Shanghai Institute of Ceramics, Chinese Academy of Sciences,
Shanghai 200050, China.
★
Correspondence and requests for materials should be addressed to:
wengwj@zju.edu.cn and hanweiqiang@nimte.ac.cn
1
Supplementary Figure S1. SEM images (A-D) and the corresponding
statistical data for the size of SrTiO3 nanocrystals (E-H) synthesized with
morphology evolution from cube to truncated rhombic dodecahedron
upon increasing the amount of 1,3-propanediol added: (A) 0 ml, (B) 1 ml,
(C) 3 ml and (D) 5 ml. Scale bars in (A-D), 200 nm. At least 100 particles
per sample were counted to obtain the average size.
2
Supplementary
Figure
S2.
TEM
characterizations
of
typical
morphology of SrTiO3 cube and truncated rhombic dodecahedron. SEAD
patterns (A, C) recorded from the corresponding single nanocrystal
(insets) viewed along [001] and [001] orientation, respectively and their
corresponding high-resolution TEM (HRTEM) images (B, D). The insets
in (B) and (D) are the corresponding Fast-Fourier-transform-filtered (FFT)
patterns.
3
Supplementary Figure S3. XRD patterns of different SrTiO3
nanocrystals. A standard XRD pattern of SrTiO3 is also provided.
4
Supplementary Figure S4. Optimal density (OD) values of salmine
protein solutions: original protein solution without addition of
nanocrystals before (A) and after (B) centrifugation, (C) the supernatant
of protein solution with addition of nanocrystals after centrifugation. The
absorbance (OD) values are measured by using bicinchoninic acid assay
(BCA) reagent at 560 nm.
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Supplementary Figure S5. (A) Low-magnification SEM image of
truncated rhombic dodecahedra SrTiO3 nanocrystals after adsorption of
salmine, as indicated by the arrow head. (B) HRTEM image recorded
from the boxed region of top right inset shows that the protein adsorb on
(100) facet. The bottom right inset shows the FFT pattern recorded from
the corresponding rectangular area. (C) High angle annular dark field
scanning transmission electron microscopy (HAADF-STEM) image of
the same typical nanocrystal with protein adsorption in (B) and a line
scan EDS profile (D) along the arrow. From the line scan EDS profile (D),
the signal intensities of U and N elements increase first then decrease at
the interfaces further demonstrates the protein adsorption. The protein
was stained by uranyl acetate (UA, 1%) after adsorption for TEM.
6
Supplementary Figure S6. Surface atomic structures of (100) and (110)
facets. HAADF (A, C) and the corresponding Bright-field (B, D) images
of the typical edge of the surface-reconstructed (100) and (110) surfaces,
along [001] and [1-10], respectively.
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εi (kJ/mol)
σi ( nm)
qi (e)
Sr
0.36227
0.33943
1.331
Ti
0.00601
0.34467
2.662
O
0.07361
0.36819
-1.331
Atom
Supplementary Table S1. Lennard-Jones parameters and atomic charges
of SrTiO3 surfaces used in this work.
8