1
2
3
4
5
6
SUPPLEMENTARY INFORMATION
Figure S1. Neighbor joining tree based on Tajima-Nei distance method. Numbers refer
to percentage bootstrap values based on 1000 replications. Bar, 2% sequence divergence.
1
1
2
3
4
5
Figure S2. Maximum parsimony tree generated using Close-Neighbor-Interchange
algorithm to search for the best tree. The numbers refer to percentage bootstrap values
based on 1000 replications. Bar, 20 substitutions.
2
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
S3. Detailed taxa and references corresponding to other free-living and
endosymbiotic Enterobacteriaceae used in phylogenetic analysis
In addition to the taxa described in the Materials and Methods section the following
represent other endosymbiotic Enterobacteriaceae included in the analysis: a
representative of R-type secondary symbionts (Moran et al., 2005): Candidatus Serratia
symbiotica (AY136139); a representative of T type secondary symbionts (Moran et al.,
2005): Candidatus Hamiltonella defensa (AY136141); a representative of U type
secondary symbionts (Moran et al., 2005): Candidatus Regiella insecticola (AY296734);
representatives of cluster A mealybug endosymbionts (Thao et al., 2002): symbiont of
Dysmicoccus brevipes (AF476103), symbiont of Dysmicoccus neobrevipes (AF476104),
symbiont of Planococcus citri (AF476107); representatives of cluster C mealybug
endosymbionts (Thao et al., 2002): symbiont of Antonina crawii (AB030020), symbiont
of Antonina pretiosa (AF476101), symbiont of Australicoccus grevilleae (AF476099);
representatives of cluster D mealybug endosymbionts (Thao et al., 2002): symbiont of
Paracoccus nothofagicola (AF476109), symbiont of Cyphonococcus alpinus
(AF476102); a representative of cluster E mealybug endosymbionts (Thao et al., 2002):
symbiont of Planococcus citri (AF476107); a primary endosymbiont of the tsetse fly:
Wigglesworthia glossinidia (AF022875); a secondary endosymbiont of the tsetse fly:
Sodalis glossinidius (AM237373); a primary endosymbiont of aphids: Buchnera
aphidicola (Y11973); a secondary endosymbiont of psyllids: symbiont of Bactericera
cockerelli (AF263557); Other free-living, animal- or plant-associated Enterobacteriaceae
include the following: a Gammaproteobacterium isolated from a spider, Aranicola
proteolyticus (U93263). representatives of Serratia: S. entomophila (AJ233427), S.
odorifera (AJ233432); representatives of Pectobacterium: P. carotovorum (DQ886670),
P. atrosepticum (AF373179), P. wasabiae (AJ223408); representatives of Yersinia: Y.
pseudotuberculosis (Z21939), Y. pestis (AJ232233); representatives of Edwardsiella: E.
tarda (AB050831), E. ictaluri (AF310622); and other free-living Gammaproteobacteria:
Escherichia coli (X80724), Salmonella typhimurium (Z49264), Enterobacter sp. NT N71
(AB167000), Xenorhabdus beddingii (D78006).
ADDITIONAL REFERENCES
Moran NA, Russell JA, Koga R, Fukatsu T (2005). Evolutionary Relationships of Three
New Species of Enterobacteriaceae Living as Symbionts of Aphids and Other Insects.
Appl. Environ. Microbiol. 71: 3302-3310.
Thao ML, Gullan PJ, Baumann P (2002). Secondary ({gamma}-Proteobacteria)
Endosymbionts Infect the Primary ({beta}-Proteobacteria) Endosymbionts of Mealybugs
Multiple Times and Coevolve with Their Hosts. Appl. Environ. Microbiol. 68: 3190-3197.
3