-1- Advanced Environmental Biotechnology II Example questions Classroom test 1 Answering these questions will help with the classroom test. The test will be multiple choice. 1. When many small units are added together what do we call them? 2. What are functional groups? Include an explanation of polar and nonpolar molecules. 3. Give four examples of biological macromolecules. 4. What do we call the polymers of amino acids? 5. How many different amino acids are there? 6. What makes the amino acids different? 7. Amino acids can be used for the synthesis of proteins. A. True B. False 8. Which of the following is an example of a polymer? A. nucleotide B. amino acid C. monosaccharide D. polysaccharide E. fatty acid 9. What is the chemical structure of amino acids and proteins, what is a peptide bond, and what are the levels of structure of proteins? 10. How many different amino acids are there? 11. What makes proteins different from each other? 12. What is the molecular structure of deoxyribonucleic acid (DNA)? What are the parts of DNA? 13. Explain how DNA can give protein structure. 14. What are three types of ribonucleic acid (RNA) and what do they do? 15. The normal process of molecular biology can be described as: A. translation of DNA to RNA followed by transcription of RNA into protein B. transcription of RNA into DNA followed by translation of DNA into protein C. transcription of DNA into RNA followed by translation of RNA into protein D. translation of RNA from protein followed by replication of DNA from RNA 16. Describe what enzymes are. You need to say at least three things. 17. Define enzyme, describe the chemical makeup and explain why they are important for cellular metabolism. 18. An enzyme is a “biological catalyst” that falls into which classes of molecules? A. nucleic acids B. lipids C. carbohydrates Advanced Environmental Biotechnology II Example Test Questions -2D. proteins 19. Describe the levels of protein structure 20. Which of the following is not a component of DNA? A. adenine B. guanine C. phosphate D. thymine E. uracil 21. At the beginning of translation is the codon AUG which codes for methionine. What is another name for this codon? 22. What is the name of a piece of DNA which codes for a protein? 23. What is transcription? 24. Give an example of a gene product? 25. What is the enzyme which catalyzes transcription? 26. How do the genes that contain the code for proteins differ from the genes from tRNA and rRNA? 27. Describe the structure of prokaryote ribosomes. 28. What is translation? 29. Bacteria have ______ as their genetic material. A. RNA B. DNA C. Protein D. All of the above 30. Why do people study nucleic acids from the environment? Why don’t they culture microorganisms instead? 31. What are two main ways of getting nucleic acids from the environment? Describe the advantages and disadvantages of each. 32. When people study the nucleic acids of the environment, they often study one type of molecule. What is this molecule? Why is this studied so often? 33. Why are microorganisms important in an ecosystem? 34. What are the steps used in the polymerase chain reaction (PCR)? 35. Why do people use PCR? 36. Which base is not found in DNA? A. adenine B. thymine C. cytosine D. uracil 37. The process of creating mRNA from DNA is called A. replication B. transcription Advanced Environmental Biotechnology II Example Test Questions -3C. translation D. transposition 38. The process of creating a protein from mRNA is called A. replication B. transcription C. translation D. transposition 39. The enzyme responsible for transcription is called A. DNA polymerase B. Transcriptase C. RNA polymerase D. Reverse transcriptase 40. Ribosomes are made of ____________. A. DNA and RNA B. Lipids and Proteins C. RNA and Protein D. Carbohydrates and RNA 41. Which of the following is a start codon? A. UAA B. AAA C. AUG D. UUU 42. tRNA is responsible for transporting _________ to the ribosome. A. amino acids B. monosaccharides C. DNA D. mRNA 43. Each tRNA has a ____________ that allows it to match up with the correct mRNA codon. A. DNA sequence B. Anticodon C. Terminator D. Locator 44. The bond that is created between amino acids is called a ____________________bond. A. ionic B. diester C. peptide D. hydrogen 45. The enzyme responsible for copying DNA is called __________. Advanced Environmental Biotechnology II Example Test Questions