GUIDELINES FOR MINIMUM INFRASTRUCTURE
FACILITIES TO BE CREATED BY THE MANUFACTURERS
OF BIOPESTICIDES
INDEX
Sl.
No.
1
2
3
Particulars
Guidelines for minimum infrastructural facilities to be created by
the manufacturers of microbial biopesticides (Antagonistic fungi,
Entomopathogenic fungi, Antagonistic bacteria and Entomotoxic
bacteria).
Guidelines for minimum infrastructural facilities to be created by
the manufacturers for Baculoviruses (NPV, GV).
Guidelines for minimum infrastructural facilities to be created by
the manufacturers of botanical biopesticides (Pyrethrum,
Azadirachtin, Cymbopogon etc.).
Page
No.
2-3
4-5
6-7
1
Annexure-A
Guidelines for minimum infrastructure facilities to be created by
the manufacturers of microbial biopesticides (Antagonistic fungi,
Entomopathogenic fungi, Antagonistic bacteria, Entomotoxic
bacteria).
Sl.No.
MANPOWER REQUIREMENT
1
2.
Quality Control Biologist
Sufficient personnel to supervise production, maintenance,
stores etc.
GENERAL REQUIREMENT
1.
Production, Mixing & Drying Room and Formulation Unit
for Antagonistic fungi/ Entomopathogenic fungi
Inoculation, Fermentor, & Sterilization
Room and
Formulation unit for Antagonistic bacteria/ Entomotoxic
bacteria.
Packaging and storage room
Quality Control laboratory
Protective clothing
Respiratory devices
First Aid Measure
Waste disposal arrangement in compliance with Pollution
Control norms
2.
3.
4.
5.
6.
7
8.
1.
2.
3.
4.
5.
6.
7.
8.
PLANT EQUIPMENT REQUIREMENT
Plant Fermentor with all accessories / Bioreactor /
Shaker
Steam Boiler
Chilling plant
Air compressor
RO/ Softener (water treatment plant)
Distillation Unit
Micro centrifuge
Magnetic stirrer
2
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
300 and 160  Sieves
Electronic Weighing Balance
Blender/Homogenizer
Vortex
Vibro-screen
Autoclavable bag
Large air tight container
Pouch sealing machine
Box strapping machine
Racks and cabinet
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
LABORATORY EQUIPMENT/ INSTRUMENT
REQUIREMENT
Autoclave
Water bath
Shaking incubator
Refrigerator
Thermo hygrometer
U. V. light
BOD Incubator
Hot Air oven
Laminar Flow
pH meter
Balance (2-3 decimal places)
Vacuum pump
Hot plate
Deep freezer
Spirit
Microscope and all accessories
Glassware viz. Conical Flasks, Test tubes, Beaker etc.
Petri dishes
Titanium inoculating needles
Pipette fillers
Pipettes (0.1 ml to 20 ml)
Haemocytometer
Colony counter
Note:
These are the general requirements of minimum infrastructure to be created by
the manufacturers. However, for specific microbial biopesticides formulation(s)
and their quantum of production, requirement of manpower, space, equipment/
instrument may be needed.
3
Annexure-B
Guidelines for minimum infrastructure facilities to be created by
the manufacturers of Baculoviruses (NPV, GV).
Sl.No.
MANPOWER REQUIREMENT
1
2.
Quality Control Biologist
Sufficient personnel to supervise production, maintenance, stores etc.
GENERAL REQUIREMENT
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
Post culture production room with temperature and humidity control
Moth ovi-position room with temperature and humidity control
Production room with temperature and humidity control
Diet preparation room
Virus processing lab.
Quality control lab.
Formulation unit
Cold storage
Washing and sterilization facility
Stores room
Protective clothing
Respiratory devices
First aid measures.
Waste disposal arrangement in compliance with Pollution Control norms
1.
2.
3
4.
5.
6.
7.
8
9
9.
11.
12.
13.
14.
15.
16.
EQUIPMENT/INSTRUMENT REQUIREMENT
Diet preparation machine (mixer)
Diet dispenser.
Multi channel pipette
Vacuum pump with an aspirator
Blenders
Multipurpose centrifuges
B.O.D. incubator or an incubator room or an environmental chamber
Scale balance
Digital balance
Haemocytometer shallow depth Counting chamber
Hot air oven.
Compound research microscope.
Zoom microscope.
Vortex mixers
Tally counters
Electric stove.
4
17.
18.
19.
20.
21.
22.
23.
24.
25.
Autoclave
Shaker.
Water distillation unit.
Air conditioners
Humidifiers
Oviposition iron cage
Washing machine
Racks and cabinet
Refrigerators
Note: These are the general requirements of minimum infrastructure to be created by
the manufacturers. However, for specific baculovirus formulation(s) and their quantum
of production, requirement of manpower, space, equipment/ instrument may be needed.
5
Annexure-C
Guidelines for minimum infrastructure facilities to be created by
the manufacturers of botanical biopesticides (Pyrethrum,
Azadirachtin, Cymbopogon etc.)
Sl.No.
MANPOWER REQUIRMENT
1.
2.
Quality Control Biologist
Sufficient personnel to supervise production, maintenance, stores etc.
GENERAL REQUIREMENT
1.
2.
3.
4.
5.
6.
7.
8.
9.
Extraction room
Production room
Formulation unit
Packing and storage room
Quality control laboratory
Protective clothing
Respiratory devices
First aid measures
Waste disposal arrangement in compliance with Pollution Control norms
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
PLANT EQUIPMENT/INSTRUMENT REQUIREMENT
Phase separation vessels
Decanter
Mixing vessels with stirrer
Centrifuge
Soxhlet apparatus
Electric oven with thermometer
Chemical measuring cylinders
Chemical transfer pumps
Filter pressure pump
Measuring cans
Electric weighing machine
Counter scales
Filtration assembly
Water distillation unit
Wrist action shaker
Blender
Vortex
Large air tight containers
Material filling trays
Storage tank
Filling machine
6
22.
23.
Sealing machine
Packing accessories
LABORATORY EQUIPMENT/INSTRUMENT REQUIREMENT
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
Note:
HPLC System
pH meter
Spectrophotometer
Refrigerator
Thermometer
Microscope
Autoclave
U. V. Lamp
Abel flash point Apparatus
Incubator
Glassware viz. Conical Flasks, Test tubes, Beaker, Separating funnel,
Funnels, Glass tubes, etc.
Pipette fillers
Pipette
Burette
TLC apparatus & accessories
Micro-syringes
These are the general requirements of minimum infrastructure to be created by
the manufacturers. However, for specific botanical biopesticides formulation(s)
and their quantum of production, requirement of manpower, space, equipment
/instrument may be needed.
7