Career Portfolio provided by Beyond.com
Scientist
Member Number: 39892012
Lewisville, TX 75067
Portfolio: http://www.beyond.com/people/39892012
Professional Information
Job Function: Science & Biotech, Research
Education: 4-Year College Degree
Experience: 21+ Years
Employment: Any Position Type
Salary: $50-120k
Citizenship: U.S. Citizen
Resume
Work Phone: ***
Experience:
Mass Spec Scientist, Reata Pharmaceuticals, Irving, Texas – September *** - present
Product Development, Drug Metabolism and Pharmacokinetics Group:
* Perform routine bioanalysis of samples from preclinical studies utilizing Waters triple quadrupole and time-of-flight mass
spectrometers (Xevo TQD, TQ-S, and QTof) and Acquity UPLC for Reata’s novel class of oral anti-inflammatory drugs,
Antioxidant Inflammation Modulators (AIMs – potent activators of the biological transcription factor Nrf2); and responsible
for establishing methodologies and analytical assays for these anti-inflammatory drugs and drug metabolites.
* Perform microsomal incubations for studying the metabolic profiles of advanced compounds prior to IND submission.
* Assess maximum solubility limits of compounds in different cell culture media used in genotoxicity studies and other cell
based assays using filtration and LC-MS/MS.
* Determine stability of compounds in dosing formulations used in toxicity and pharmacokinetic studies in animals.
* Sample preparation for these analyses includes homogenization techniques for various tissues and solid-phase or liquidliquid extraction in a low-throughput format.
Senior Associate Scientist, Amgen Incorporated, Thousand Oaks, California - March *** – September ***
Department of Pharmacokinetics and Drug Metabolism, Small Molecule Bioanalytical Group:
* Developed rugged bioanalytical methods utilizing high throughput LC-MS/MS techniques (Tecan Genesis, Eppendorf
epMotion, and Tomtec workstations with Sciex API ***/***/*** analysis using Analyst Software) for small molecule drug
candidates and peptide-based biopharmaceuticals in various biological matrices (including plasma and tissue/tumor
homogenates).
* Conducted LC-MS/MS assays in support of cancer, neuroscience, metabolic disorders, and inflammation discovery
research, preclinical pharmacology, clinical pharmacokinetics (discovery support with *** hour turnaround times), and
toxicology under SOP and GLP compliance.
* Responsible for PK data releases generated using non-compartmental analysis within the Watson laboratory information
management system.
Scientist
* Accountable for distribution and presentation of data to project teams and project team status to the department.
* Performed HPLC-APCI/ESI-MS/MS assay development and refinement, validation implementation, assay protocol and
standard operating procedure preparation, as well as documentation summaries of studies and analyses.
* Routinely developed UHPLC methods utilizing reduced cycle times with ultra-high pressure pumps and solid/fused-core
columns at controlled temperatures for same-day turn-around time for data release of samples received.
* Maintained automation and analytical instrumentation under SOP and GLP compliance.
* Performed computer related system installations and operational qualifications (IQ/OQ) for validating the operation of
new mass spectrometers and peripherals using Analyst v*** and v*** under GLP compliance.
Senior Research Associate, Lexicon Pharmaceuticals Incorporated (formally Lexicon Genetics), The Woodlands, Texas November *** - March ***
Cardiology - In-Vivo ADME Group:
* Responsible for project management and analytical method development and analysis by LC-MS/MS (Sciex API***) of
samples generated from in-house murine pharmacokinetic and absorption studies of investigational new small molecules
designed to inhibit Lexicon's in-vivo validated functional targets.
* Optimized rapid throughput sample preparation.
* Responsible for determination of compound solubility in dosing vehicles and the formulation of dosing solutions.
* Developed assays for identification of potential metabolites from in-life studies.
Pharmaceutical Discovery - HTS Group:
* Responsible for developing and validating enzymatic, receptor-ligand binding, and metabolic high throughput screening
(HTS) assays for implementation into automation (Biomek FX, ***).
* Responsible for developing screening assays on high throughput analytical instrumentation, including Tecan Ultra ***,
Molecular Devices, and Packard Fusion plate readers for absorbance, fluorescence, and fluorescence polarization
assays; and the Packard TopCount NXT for radioligand based assays.
* Prepared assayable genomically derived proteins from yeast, bacterial, and mammalian cells.
* Optimized the use of novel enzyme substrates and ligands of orphan receptors.
* Became very adept in liposome and radiolabeled substrate preparation and fraction collection.
* Performed low throughput screening and substrate purification using HPLC with various detection methods.
Research Associate II, Amgen Incorporated, Thousand Oaks, California - April *** - October ***
Department of Pharmacokinetics and Drug Metabolism, in-vitro ADME Group:
* Established the use of primary culture hepatocytes for use in cell-based bioassays at Amgen.
* Responsible for equipping an existing laboratory for mammalian cell culture procedures.
* Developed assays to determine the metabolic competence of isolated primary culture hepatocytes for use in in-vitro
metabolism investigations and performed incubations to evaluate biotransformation kinetics.
* Developed analytical methods for analysis of primary hepatocyte and microsomal incubations using the HPLC Waters
Alliance system (with photo diode array and in-line radiometric detection: ß-RAM flow scintillation analyzer), Micromass
LCZ LC-MS, Sciex API*** LC-MS/MS, as well as liquid scintillation analysis.
* Optimized sample preparation procedures including solid-phase and liquid-liquid extractions and protein precipitation.
* Developed and performed fluorescent high-throughput P*** inhibition assays with recombinant enzymes using a
Spectramax plate reader with Softmax Pro software; and automated using the Tecan Genesis RSP *** workstation.
* Prepared microsomal and other subcellular fractions from whole livers and determined protein content, total cytochrome
P*** concentration, and enzymatic activity using UV-VIS and LC-MS and prepared standard operating procedures and
test methods for these assays.
Scientist
Laboratory Associate, CEDRA Corp., (Currently known as Worldwide Clinical Trials Drug Development Solutions) Austin,
Texas - March *** - April ***
In-vitro Drug Metabolism Division:
* Performed microsomal incubation procedures for in-vitro assessment of cytochrome P*** enzyme kinetics.
* Responsible for the operation and maintenance of the Micromass Quattro II LC-MS/MS for the analyses of in-vitro
metabolism evaluations, and developed LC-MS/MS analytical methods for the assessment of specific P*** isoforms.
* Validated and wrote standard operating procedures for these assays following stringent SOP and GLP guidelines.
* Responsible for the technical review and reporting of data produced from inhibition studies of investigational new test
compounds.
Bioanalytical Laboratory:
* Conducted bioanalytical support for toxicology and PK studies by developing, validating, and performing assays for
preclinical and clinical studies, using various techniques and high throughput methodologies.
* Performed liquid-liquid extraction techniques as well as solid-phase extraction sample preparation procedures.
* This position required a working knowledge of mass spectrometry, HPLC, spectrophotometry, as well as other laboratory
instruments and computer programs.
* Developed high throughput extraction and aliquotting methods using automation equipment, including Tomtec and
Multiprobe workstations.
* Responsible for the GLP validation of bioanalytical assays using manual and automated extraction techniques.
* Performed immunoassays; including RIAs, ELISAs and chemiluminescent assays (using ORIGEN detection
technology).
* Used the Franz dissolution apparatus for studying permeability of topical preparations.
* Responsible for writing standard operating procedures and final reports for these studies.
HepCyte Cell Culture Team:
* Performed hepatocyte isolations from various species and prepared these primary isolations for culture kits.
* Responsible for the culturing of these cells for metabolism studies.
* Responsible for laboratory animal maintenance and upkeep.
* Assisted in the upkeep of CACO*** cells for absorption/permeability studies.
* These cell culture procedures required knowledge of sterile technique and media preparation.
Quality Control Data Management Unit:
* Responsible for processing raw data from various data acquisition systems.
* Performed GLP QC checks on bioanalytical assay reports, data tables, and final assay validations and reports.
* Others duties included GLP Quality Control/Quality Assurance of laboratory equipment which included daily inspections
and monthly laboratory audits.
Laboratory Technician, US Army Corps of Engineers Waterways Experiment Station, Vicksburg, MS - June *** – Jan. ***
Hazardous Waste Research Center, Wetlands Research Program:
* Conducted bench-scale column studies of adsorbents in attempt to remove industrial contaminants from water.
* Evaluated contaminated soils and dredged material with various tests and performed solidification/stabilization
treatments for these contaminated solids.
* Responsible for final report preparation and data entry used for generation of tables and graphs.
Ecosystem Research and Development, Wetlands Research Program:
Scientist
* Conducted greenhouse tests pertaining to plant uptake of heavy metals and other pollutants to study plant bioaccumulation of contaminants (heavy metals, organics, explosives) for environmental impact assessments and phytoremediation of contaminated soils.
* Conducted environmental contaminant uptake studies on wetland-dependent plants and organisms.
* Performed field collections of soils, dredged material, and waters for various studies including assessment of
contaminant mobility in artillery impact areas.
* Performed invertebrate bioassays and toxicity tests.
* Prepared environmental samples from the field as well as those generated in-house for analysis of heavy metals and
pesticide residues. This required knowledge of various digestion techniques of soil, animal, and plant material.
* Other duties included report preparation, greenhouse maintenance, test animal upkeep, and various lab practices.
Substitute Teacher, Hinds Community College, Vicksburg, Mississippi Freshman Biology, Spring ***
Substitute Teacher, Porters Chapel Academy, Vicksburg, Mississippi Junior-Senior Chemistry, Fall ***
Professional Membership:
American Society for Mass Spectrometry, *** - present
Education:
Mississippi College, Clinton, Mississippi - January *** - May ***
* Completed Bachelor of Science in Biological Science (*** GPA)
* Graduate work toward a Master of Combined Science (Chemistry/Biology)
Mississippi State University, Mississippi State, Mississippi - August *** - December ***
* Work towards Bachelor of Science degree
Additional Training:
* MassLynx ***, QuanLynx, MetaboLynx, and MassFragment Software Fundamentals Training Course from Waters
* Acuity UPLC TQD Operation and Maintenance Course (Waters Educational Services)
* Xevo Q-ToF Operation Training, Small Molecule Applications (Waters Educational Services)
* Xevo TQ-S Operation Training (Waters Educational Services)
* Good Laboratory Practice Regulations/Intro to GLPs and GLP Documentation
* AB Sciex *** QTOF Analyst Operator Training
* Sciex API***/*** Analyst Operator Training Course
* Sciex API*** (with Analyst ***) Operator Training Course
* Introduction to Pharmacokinetics
* Blood borne Pathogens Awareness Training (with yearly refresher classes)
* Radiation Safety Training for Users (with yearly refresher classes)
* Laboratory (Hazardous and Bio-) Waste Management and Hood Safety Awareness Training
* AALAS Animal Care and Use Training
* *** CFR Part *** for Clinical Development
* Comparison of OECD and MHW to FDA GLP Regulations
Scientist
* Microsoft Excel Advanced Functions
* Microsoft PowerPoint Embedding and Linking
* Early Development/Medical Science Education
* Intra-functional education: Protein/Immunology, Oncology, Hematology, and Neuroscience therapeutic areas
* Regulatory Inspection Conduct Training
* Chemical Hygiene/Potent Compound/Hazard Communication Training
* Environmental Health and Safety Plan Training
* Laboratory Inspections Training
* Regulatory Affairs Education
* Information System Validation Training
* Validation and Application of Bioanalytical Methods Used in Support of GLP Studies and Clinical Trials SOP Training
* Cecilia Basic’s “Instruments for Accurate Mass Analysis and Quantitative Applications” training course (June ***)
* Bioanalytical Method Validation and Sample Analysis: Challenges, Solutions, Applications, Global Industry Standards,
and New Regulations Course (6th Workshop on Recent Issues in Bioanalysis, March ***, ***, San Antonio, TX)
* Introduction to Phoenix for WinNonlin version ***x & *** – hands on training from Pharsight/Certara
Publications:
Qingping Zeng, John G. Allen, Matthew P. Bourbeau, Xianghong Wang, Guomin Yao, Seifu Tadesse, James T. Rider,
Chester C. Yuan, Fang-Tsao Hong, Matthew R. Lee, Shiwen Zhang, Julie A. Lofgren, Daniel J. Freeman, Suijin Yang,
Chun Li, *** ***, Xin Huang, Douglas Hoffman, Harvey K. Yamane, Christopher Fotsch, et al. Azole-based inhibitors of
AKT/PKB for the treatment of cancer Bioorganic & Medicinal Chemistry Letters, Volume ***, Issue 5, 1 March ***, Pages
***
Qingping Zeng, Matthew P. Bourbeau, G. Erich Wohlhieter, Guomin Yao, Holger Monenschein, James T. Rider, Matthew
R. Lee, Shiwen Zhang, Julie Lofgren, Daniel Freeman, Chun Li, *** ***, Xin Huang, Douglas Hoffman, Harvey Yamane,
Andrew S. Tasker, Celia Dominguez, Vellarkad N. Viswanadhan, Randall Hungate, Xiaoling Zhang. ***Aminothiadiazole
inhibitors of AKT1 as potential cancer therapeutics Bioorganic & Medicinal Chemistry Letters, Volume ***, Issue 5, 1
March ***, Pages ***
Averett, D.E.; Zappi, P.A.; Tatem, H.E.; Gibson, A.C.; ***, E.A.; Tate, N.S.; Graham, S.L.; Kenna, B.T.; Yaksich, S.M. ***
Buffalo River Dredging Demonstration. Prepared by U.S. Army Engineer Waterways Experiment Station and Buffalo
District, U.S. Army Corps of Engineers. Technical Report EL***
Posters: (for external presentation)
Anna Akrami; Shubhada Kulkarni; *** Williams; Craig Marshall; Beth ***; Bernd Bruenner; Keyang Xu; John Laycock; Julie
Flynn; Krys Miller. (Amgen Inc., Thousand Oaks, CA) The Development of a *** pg/ml Robust Analytical Procedure to
Support Microdosing Studies. (*** ASMS Annual Conference on Mass Spectrometry)
Sajeevi Gunasekera, Craig Marshall, Beth ***, and Paul Burke. (Amgen Inc., Thousand Oaks, CA) Bradykinin (B1)
Peptide Antagonist Acylation in Poly(lactide-co-glycolide) (PLG) Microspheres. (Presented at Pepperdine University,
October ***)