Supplementary table 1 - List of genes and PCR primers
PCR primers were designed from EST sequences corresponding to genes indicated, with exception of Adh1, where the GenBank sequence was used to design primers. Location of primers within the corresponding GenBank sequence is indicated where appropriate. The primers were as
listed below, but were equipped with a T3 extension: 5'-AATTAACCCTCACTAAAGGG-3' (primer 1) or a T7 extension: 5'-GTAATACGACTCACTATAGGGC-3' (Primer 2) at their 5'-ends.
Primer
set
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
1
Gene or closest GenBank homolog
Pyruvate dehydrogenase E1 beta subunit isoform 1
EST similar to methionine adenosyltransferase 1
Proline-rich protein
Nucleotide pyrophosphatase precursor
Nucleoside-diphosphate kinase
Acetyl-CoA C-acyltransferase
Hydroxymethyltransferase
Stearoyl-ACP desaturase
Caffeic acid 3-O-methyltransferase
Beta-expansin
Glyceraldehyde 3-phosphate dehydrogenase subunit A
Lipid body-associated major protein L3
Glycine-rich RNA binding protein
Glycine-rich RNA binding protein
Plasma membrane H+-transporting ATPase
Plasma membrane H+-transporting ATPase
Glutamyl-tRNA reductase precursor
3-phosphoshikimate 1-carboxyvinyltransferase
Protochlorophyllide reductase precursor
Alcohol dehydrogenase (Adh1), 5'-untranslated
Alcohol dehydrogenase (Adh1), 5' coding
Alcohol dehydrogenase (Adh1), 3'-end of the gene and 3'-UTR
Species
maize
rice
maize
rice
rice
Mangifera indica
arabidopsis
rice
maize
rice
maize
maize
maize
maize
maize
maize
rice
maize
barley
maize
maize
maize
GenBank Entry
AF069908
P46611
X60432
AAA67067
Q07661
CAA53078
CAB10172
Q40731
M73235
AAB61710
X07157
M17225
AF034945
AF034945
X85805
X85805
O48674
X63374
X15869
M32984
M32984
M32984
Blast pLOG Primer 1 and position within GenBank sequence
N
X
N
X
X
X
X
X
N
X
N
N
N
N
N
N
X
N
N
109
11
82
48
27
14
55
24
47
18
63
66
27
27
84
84
22
83
49
5'-CCAAAGACACCAGAAAACAT-3'
5'-AAATAGCTGTGGCCTGTAGC-3'
5'-ACTGATTAAGCAAGCTACACG-3' (nt. 1249-1229)
5'-AGCATCAGTCAATTAAGG-3'
5'-CGGTACATTAGAAAACAGGG-3'
5'-ATGACACTGTATTGTCGCC-3'
5'-TCTCATATGGTTCCC GC-3'
5'-CGACAGACAAAGTACGG-3'
5'-CACATGGCAGAGACAATTC-3' (nt. 2438-2420)
5'-TATTCGTTCTGACAGCAGC-3'
5'-ACATTTGGCCGGTATATTC-3' (nt. 1285-1264)
5'-ATGTACAAGTACCTGACGG-3' (nt. 310-328)
5'-TTCGTCACCTTCTCCACG-3' (nt.228-245)
5'-TTCGTGTTCAGGTTCTGG-3' (nt. 33-50)
5'-AACGACAAGAGCAGCTACC-3' (nt.2885-2903)
5'-AACGACAAGAGCAGCTACC-3' (nt.2885-2903)
5'-ACCAAGAAGATGAGGAGAGC-3'
5'-ACTACTGCATCATCACG-3' (nt. 1145-1161 )
5'-TTTCCAGAAGTTCGTCACC-3'
5'-AGATCCAAACAGTCACTTAGG-3' (nt. 38-58)
5'-CATCTCTTCCTCCTTTAGAGC-3' (nt. 1051-1071)
5'-GCTGTCTAGTTTGTGATCTGG-3' (nt. 4207-4227)
The first two nucleotide of the primer (5'-GA...) correspond to a cDNA clone in our collection, but not to the GenBank entry positions 3310-3309, which are AG.
Primer 2 and position within GenBank sequence
5'-TTAGACGGGAAGAGAGATCA-3'
5'-GTGAAGCCACTCAAGTCG-3'
5'-ATCAACATCTACCTCCCC-3' (nt. 934-951)
5'-ATGGACCTCGTTTTCAGG-3'
5'-AGATCGCTCTCTGGTTCC-3'
5'-TCTTCTAAATGAAATGAAGCG-3'
5'-ACATCCAGAAGGAGTACGG-3'
5'-CAGCTGGGTATACGGTAGG-3'
5'-CTACATCTACGCCAACG-3' (nt. 2099-2115)
5'-AGGAGTTCGTGATTGCGT-3'
5'-GTCTCCTCCACCATCGAC-3' (nt. 1012-1029)
5'-CAAAGAGAGACGCATACAGC-3' (nt. 673-654)
5'-CACTAACAACATCAGAGGAGC-3' (nt. 691-671)
5'-TGGAGAAGGTGACGAAGC-3' (nt. 243-226)
5'-GAAACTCCCTAGAAAGACGG-3' (nt. 3206-3187)
5'-GATCACCGGCTAGTTAGAGG-3' (nt. 3308-3291)1
5'-ACCAAGAAGATGAGGAGAGC-3'
5'-TGTATTTCCTCAGCACAAGC-3'
5'-AATTACAGGCTCTTCTCCG-3'
5'-ATTTCATAAACCCAACAAGG-3' (nt. 367-348)
5'-AATAAGTTCACCACGATTGC-3' (nt. 1410-1391)
5'-AATCCTCTCCAATTCTCACC-3' (nt. 4576-4557)