Contract Sample Evaluation Checklist
Revision A.03
PURPOSE: The SensíQ Technologies Contract Services scientists need to know how
many interactions are to be measured, what kinds of analyses are needed, and some
specifics of the biomolecules to be analyzed. Please provide answers (if available) to
the following questions:
Number of interactions to be analyzed:
_________
If per day, specify desired throughput per day _________
Type of analysis: (check all that apply)
Affinity ________
Kinetics________
Thermodynamic ______
Multisite-binding ______
Diffusion Coefficient/Molecular Weight Determination _________
The following checklist ensures that the required information is conveyed in order to
enable accurate evaluation of samples. This checklist covers a single interaction pair.
Include one checklist for each set of biomolecular pairs to be analyzed.
IMPORTANT! Please provide as much information as possible as this allows us to best
understand and develop the assay.
STRUCTURAL AND FUNCTIONAL CHARACTERIZATION:
List references for structural and functional characterization, or if in your possession,
these may be emailed to aaron.martin@sensiqtech.com.
SAMPLE PREPARATION GUIDELINES:
 Avoid all amine containing buffers.
 Where possible, provide as a concentrated sample (e.g. 2-10uM)
 Where possible, avoid free thiols (mercaptoethanol is sometimes a requirement
for stability).
Contract Sample Evaluation Checklist
Page 1 of 4


Avoid high molarity (e.g. > 100mM) buffers.
High concentrations of interactants are required if characterized by low
affinity.
Please provide a general description of the assay
Contract Sample Evaluation Checklist
Page 2 of 4
INTERACTION PARTNER A:
Scientific Name:
Available Concentration:
Available Amount:
Purity:
Relative Tendency to Precipitate:
(Preventative Measures)
Relative Tendency to Form Multimers:
(Preventative Measures)
List all components of Sample Buffer:
Short and Long Term Storage Directions:
Biomolecule Type (e.g. protein):
Molecular Weight:
Isoelectric Point:
Monomer, Dimmer, or Multimer:
Single Chain, or Multi-Chain:
List Functional Groups, Tags, Metal Ions:
Number of Binding Sites:
Recombinant, or Fusion Protein:
Estimated Binding Affinity:
Chemical Robustness:
Known Inhibitors:
Required Additives for Binding:
(e.g. reducing agent, ATP, etc.)
If Multimolecular complex, describe Complex:
Location where normally found:
Contract Sample Evaluation Checklist
Page 3 of 4
INTERACTION PARTNER B:
Scientific Name:
Available Concentration:
Available Amount:
Purity:
Relative Tendency to Precipitate:
(Preventative Measures)
Relative Tendency to Form Multimers:
(Preventative Measures)
List all components of Sample Buffer:
Short and Long Term Storage Directions:
Biomolecule Type (e.g. protein):
Molecular Weight:
Isoelectric Point:
Monomer, Dimmer, or Multimer:
Single Chain, or Multi-Chain:
List Functional Groups, Tags, Metal Ions:
Number of Binding Sites:
Recombinant, or Fusion Protein:
Estimated Binding Affinity:
Chemical Robustness:
Known Inhibitors:
Required Additives for Binding:
(e.g. reducing agent, ATP, etc.)
If Multimolecular complex, describe Complex:
Location where normally found:
Contract Sample Evaluation Checklist
Page 4 of 4