HPV16-E7 Expression in skin induces TSLP-1
secretion, type 2 ILC infiltration and atopic
dermatitis-like lesions
Anne-Sophie Bergot1, Nastasia Monnet1, Le Son Tran1, Deepak Mittal1, Jane Al-Kouba1,
Raymond J. Steptoe1, Michele A. Grimbaldeston2, Ian H. Frazer1* and James W. Wells1*
1
The University of Queensland Diamantina Institute, The University of Queensland,
Translational Research Institute, Brisbane, QLD, Australia;
2
Division of Human Immunology, Centre for Cancer Biology, University of South Australia
and SA Pathology, Adelaide, South Australia, Australia
* These authors share senior authorship of this work
Running title: HPV may contribute to the development of AD-like lesions
Key words:
Human papillomavirus; Atopic Dermatitis; Thymic Stromal Lymphopoitein;
innate lymphoid cells; atopic march.
Corresponding Author: Dr James W. Wells, The University of Queensland Diamantina
Institute, University of Queensland, Translational Research Institute, 37 Kent Street,
Woolloongabba, QLD 4102, Australia. Email: j.wells3@uq.edu.au; Phone: +61 7 3443 6983;
Fax: +61 7 3443 6966
Abbreviations:
Human papillomavirus (HPV); Atopic Dermatitis (AD); Thymic Stromal
Lymphopoitein (TSLP-1); innate lymphoid cell (ILC); mast cell (MC)
Supplemental Figure 1: Lesions occur independently of IL-33 and IL-25
E7 (n=7) and C57 (n=6) skin was analyzed for (A) IL-33 and (B) ST2 gene expression
by Q-PCR (** p=0.0012 and ns). (C) Representative image of E7.ST2 KO ear skin
tissue stained with H&E (scale 20m). (D) Ear skin thickness for E7.ST2het (n=5),
E7.ST2 KO (n=10), ST2het (n=6), and ST2 KO (n=9) mice, ns and **** p<0.0001. (E) E7
(n=12) and C57 (n=8) skin was analyzed for IL-25 mRNA expression by Q-PCR (**
p=0.0096). Data are pooled from 2 independent experiments and analyzed using a
Mann-Whitney t-test. Bars represent median values.