CNS membership number: S423101025A
SHP-2 promotes the maturation of oligodendrocyte precursor
cells through Akt and ERK1/2 signaling in vitro
Xiujie LIU, Yuan-yuan LI, Yong ZHANG, Yan LU, Wei GUO, Peng LIU, Jia-zhen ZHOU,
Zheng-hua XIANG, Cheng HE*
Institute of Neuroscience and Key Laboratory of Molecular Neurobiology of Ministry of
Education, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China
*Corresponding
author
E-mail: chenghe@smmu.edu.cn
Abstracts: Objective Oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes
(OLs), which are responsible for myelination. However, the molecular mechanisms of maturation
and myelination by oligodendrocytes remain elusive. The present study is aim to explore the roles
of SHP-2 in oligodendrocytes maturation. Methods In this study, we first searched the potential
tyrosine phosphatases involved in oligodendrocytes maturation using OPCs as the model. Then,
we characterized SHP-2 expression during the development of oligodendrocytes by RT-PCR,
immunostaining and western-blot. The roles of SHP-2 in olidendrocytes maturation were detected
by gene manipulations subsequently. Finally, the mechanism was examined by detecting activities
of concerned kinases. Results (1) We found that SHP-2 was persistently expressed during
developmental process of oligodendrocytes. (2) SHP-2 knock down attenuated oligodendrocytes
maturation and this effect was triiodo-L-thyronine (T3) dependent. Over-expression of SHP-2
promoted maturation in primary cultured oligodendrocytes. (3) It has been identified that SHP-2
may regulate oligodendrocytes maturation through Akt and Erk1/2 signaling. Conclusions SHP-2
promotes oligodendrocytes maturation via Akt and ERK1/2 signaling in vitro.
Keywords: SHP-2; oligodendrocyte precursor cells; maturation; Akt; ERK1/2