AP Biology Lab Report #1 - 9-25-2013 (Polyphenoloxidase Lab)
TITLE PAGE
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Include the Title of the Lab. In this lab it should include the two questions you were trying to answer.
Include the name(s) of the students submitting the lab
Include the date the lab was due
Include the name of the instructor and name of the class
INTRODUCTION
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Purpose of the lab
Include literature that you researched prior to starting the lab
Explain why we are using potato in this lab
Explain the purpose of enzymes in general ( be quick – include activation energy and metabolism)
Explain how primary, secondary, tertiary, and quaternary structure dictate the function of a protein (enzyme) – detailed.
Explain the purpose of polyphenoloxidase for plants. Include molecular purpose and adaptive (evolutionary fitness)
purpose.
Explain how/why enzyme catalyzed reactions are changed by temperature
Explain how enzymes utilize cofactors (vitamins and minerals)
Explain how enzyme action can be affected by allosteric molecules and competitive inhibitors
MATERIALS
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Include all the materials needed to complete this lab
The Title Page, Introduction, & Materials will be completed as a group effort with your lab partner. Everything after this will
be done separately.
ONE LAB PARTNER WILL COMPLETE THE ISOMER PORTION OF THE LAB.
THE OTHER LAB PARTNER WILL COMPLETE THE OTHER TREATEMENT PORTION OF THE LAB (Fruit fresh, Salt, Detergent or
EDTA).
The rest of the lab-write up will be individual.
PROCEDURE
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Step by step numbered procedure for your portion of the lab.
HYPOTHESES
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Include a NULL hypothesis and EXPERIMENTAL hypothesis for your lab.
MANIPULATE ONE VARIABLE (Independent)
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What was the one variable that you manipulated in your experiment?
PROVIDE A CONTROL GROUP
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What was your control group for each experiment? You use the control group as a comparison for the experimental
groups
KEEP ALL OTHER VARIABLES CONSTANT
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Provide 5 constants in your lab. Please number these.
PROVIDE QUANTITATIVE DATA/VERIFY THE EXPERIMENTAL RESULTS/STATISTICALLY ANALYZE RESULTS
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Explain what your quantitative data was in the lab. It is usually your dependent variable (aka the Y axis)
Explain how the spectrophotometer enabled you to get quantitative results.
Include data tables of your raw data – you can
Include another table for each experiment which includes the initial reaction rate (first minute) and overall reaction rate
for your control reaction and experimental reactions.
You should include a line graph (absorbance vs. time) – with legend/key {This must be hand drawn}
Also include a graph of the overall reaction rate vs. treatment (isomer = bar graph) , (other treatments could be line or
bar graph) {This must be hand drawn}
DATA ANALYSIS
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Analyze the data in words
CALCULATION
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Show how you calculated reaction rate (initial and overall). Show one calculation of initial reaction rate and one
calculation of overall reaction rate.
CONCLUSION
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Restate the hypotheses and then use DATA (that is correct-- NUMBERS) to help support or refute your hypotheses. You
should also refer to data tables and or graphs during the conclusion.
Explain in detail why a change in the reaction rate occurred or why it did not occur. You should refer back to your
knowledge of enzymatics/ tertiary structure and R-group interactions. (THIS IS VERY IMPORTANT!!!)
What would you have done differently if you had to do this lab again?
Include possible reasons for error – be specific – don’t just say human error guys!!!
REFERENCES
Book example
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S.S. Rao, Mechanical Vibrations, Fourth Edition, Prentice-Hall, Inc., New Jersey, 2004.
Website example
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T.J. Ward fined 15K by NFL -- Associated press
-- retrieved October 6, 2010 from
http://sports.espn.go.com/nfl/news/story?id=5655099