Supplemental Figure legend
Figure S1
Absorption spectra and light-minus-dark difference spectra (inset) of substituents of At phot1
LOV2-STK. The solid black and red lines indicate dark-adopted and poset-BL-irradiated
spectra, respectively.
Figure S2
Kinase assay in time course (A) and under different light intensities (B) of H638A, R639A and
D641A. For the time course, the samples were irradiated under 34 μmol m-2 s-1. For the varying
light intensity assay, samples were irradiated for 15 min. The arrow and arrowhead indicate
P1Nt and the substituents of LOV2-STK, respectively. The upper and lower panels indicate
phosphorimaging and CBB staining, respectively. The data for WT are the same as shown in Fig.
3
Figure S3
(A) The changes in time of the activated LOV2-STK model as simulated using Eq (1) - (4) in
Supplemental text. Black, red, blue and green lines indicate the model A, B, C and D,
respectively. (B) The ratio of total amount of substrate phosphorylation of LOV2-STK models
as simulated using Eq (5) for 1800 s.
Figure S4
Pull down assay of At phot1 LOV2 and STK. Purified His-tagged LOV2 (449-586) and
GST-tagged STK (663-995) were mixed and incubated in the dark for 45 min, 4ºC. Added the
GST resin and then removed the flow through fraction. After washing the resin, the SDS-PAGE
sample buffer was added to the resin. Samples were separated by the SDS-PAGE. Closed and
open arrowheads indicate His-LOV2 and GST-STK, respectively. FT and W indicate flow
through and wash fractions, respectively.