CHARACTERS (Period 3) 16 nucleotides (must note 3’ & 5’ ends) o 4 adenosine with phosphate & deoxyribose attached (2 old, 2 new) o 4 thymine with phosphate & deoxyribose attached (2 old, 2 new) o 4 guanine with phosphate & deoxyribose attached (2 old, 2 new) o 4 cytosine with phosphate & deoxyribose attached (2 old, 2 new) 1 DNA helicase (unwinding/unzipping) 1 RNA primase (priming) 1 DNA polymerase (adding bases/proofreading) 1 DNA ligase (joining Okazaki fragments) 1 Nuclease (deletes incorrect nucleotides) MOVEMENT You will be divided into two groups of 21. Each group of 21 students must demonstrate a choreographed representation of the four stages of semiconservative replication using each student as a chemical found during replication. Your teacher will select 5 group managers which will function as the enzymes in charge of replication. o You will model the four stages of replication: Step 1: Unzipping/unwinding DNA Start with the old twisted strand of DNA joined together with hydrogen bonds between the bases. Helicase will unwind the DNA and break the hydrogen bonds between the bases. Step 2: Priming Exposed DNA Strand Primase will add a floating/new nucleotide to each of the old strands to act as the primer. Step 3: Adding New Nucleotides DNA polymerase will add nucleotides one at a time starting on the 5’ ends. (Create a mutation for step 4.) The leading strand will be formed quickly in one continuous motion. An Okazaki fragment will form on the lagging strand because DNA polymerase will fall off before a complete strand is made. Ligase will bring in the missing nucleotide to fill in the gap between the Okazaki fragments. Step 4: Proofreading Completed Strands DNA polymerase will “proofread” each nucleotide checking that nucleotides are correctly matched. DNA polymerase will find a mutation signaling nuclease to yank the incorrect nucleotide from its position. DNA polymerase will then add the correct nucleotides and proofread again. The movement must be succinct, thoughtful, and creative. Each student must know his or her part. Be as creative as you need to be to enhance your performance (costumes, props, etc.) REQUIREMENTS Seamless flow (no abrupt stops) Music No talking/gestures Finished product of two identical strands of DNA (semiconservative replication) GRADING Group managers will assign individual intra group grades using a rubric designed by your teacher. (30 points) Teacher-judges will assign group grades and pick the winning group (extra credit). (40 points) CHARACTERS (Period 5) 14 nucleotides (must note 3’ & 5’ ends) o 3 adenosine with phosphate & deoxyribose attached (2 old, 2 new) o 3 thymine with phosphate & deoxyribose attached (2 old, 2 new) o 3 guanine with phosphate & deoxyribose attached (2 old, 2 new) o 3 cytosine with phosphate & deoxyribose attached (2 old, 2 new) o 2 extra of your choice that will be needed in step 4 1 DNA helicase (unwinding/unzipping) 1 RNA primase (priming) 1 DNA polymerase (adding bases/proofreading) 1 DNA ligase (joining Okazaki fragments) 1 Nuclease (deletes incorrect nucleotides) MOVEMENT You will be divided into two groups of 19. Each group of 19 students must demonstrate a choreographed representation of the four stages of semiconservative replication using each student as a chemical found during replication. Your teacher will select 5 group managers which will function as the enzymes in charge of replication. o You will model the four stages of replication: Step 1: Unzipping/unwinding DNA Start with the old twisted strand of DNA joined together with hydrogen bonds between the bases. Helicase will unwind the DNA and break the hydrogen bonds between the bases. Step 2: Priming Exposed DNA Strand Primase will add a floating/new nucleotide to each of the old strands to act as the primer. Step 3: Adding New Nucleotides DNA polymerase will add nucleotides one at a time starting on the 5’ ends. (Create a mutation for step 4.) The leading strand will be formed quickly in one continuous motion. An Okazaki fragment will form on the lagging strand because DNA polymerase will fall off before a complete strand is made. Ligase will bring in the missing nucleotide to fill in the gap between the Okazaki fragments. Step 4: Proofreading Completed Strands DNA polymerase will “proofread” each nucleotide checking that nucleotides are correctly matched. DNA polymerase will find a mutation signaling nuclease to yank the incorrect nucleotide from its position. DNA polymerase will then add the correct nucleotides and proofread again. The movement must be succinct, thoughtful, and creative. Each student must know his or her part. Be as creative as you need to be to enhance your performance (costumes, props, etc.) REQUIREMENTS Seamless flow (no abrupt stops) Music No talking/gestures Finished product of two identical strands of DNA (semiconservative replication) GRADING Group managers will assign individual intra group grades using a rubric designed by your teacher. (30 points) Teacher-judges will assign group grades and pick the winning group (extra credit). (40 points) Name of Group Member Being Evaluated: _______________________________________________________________ Evaluator: _________________________________________________________________________________________ COOPERATION Excellent (10 points) This group member had a positive attitude. The person often took initiative and was willing to contribute to all stages of the assignment. Adequate (7 points) This group member was a satisfactory participant. The person occasionally took initiative and did contribute when asked to do so at different stages of the assignment. Weak (5 points) This group member often complained or often was more of an obstacle. The person’s contribution was marginal during the different stages of the assignment. Adequate (7 points) This group member did an acceptable job preparing for the presentation by working on the project and/or assisting with all stages of the delivery. The person’s dedication to the group’s success was sometimes evident at different stages of the assignment. Weak (5 points) This group member did little to nothing to prepare for the presentation. The person’s dedication to the group’s success was minimal. Adequate (7 points) This group member was sometimes dependable during the different stages of the project. He or she tried to complete his or her part in a timely manner, but sometimes left others to pick up the slack. Weak (5 points) This group member was not dependable and often left others to pick up the slack. Cooperation Score: EFFORT Excellent (10 points) This group member went above and beyond in preparing for the presentation by working on the project and assisting with all stages of the delivery. The person’s dedication to the group’s success was clearly evident at all stages of the assignment. Effort Score: RESPONSIBILITY Excellent (10 points) This group member was clearly dependable at all stages of the project and could be counted on to ensure that his or her part was completed in a timely manner. Responsibility Score: OVERALL SCORE: Name of Manager Being Evaluated: _____________________________________________________________________ Evaluator(s): _______________________________________________________________________________________ LEADERSHIP Excellent (10 points) This manager was a great leader. The individual instilled pride, provided direction, and created a strong sense of team. This person also was strongly involved in the execution of the project. Adequate (7 points) This manager was a good leader. Some direction was given to the group but questions still arose. This person attempted to create a sense of team and pride in the group but was not always successful. Some involvement in the project was evident. Weak (5 points) This manager was a poor leader. The individual did not instill a sense of pride or team. The group lacked direction. This manager was not really personally involved. Adequate (7 points) This manager delegated some responsibilities to others but completed various tasks personally. When responsibilities were delegated, this person did not follow up and/or was not clear with directions. Weak (5 points) This manager did not delegate responsibilities to others and often completed tasks personally. Adequate (7 points) This manager was good at communicating ideas. This person listened somewhat to others’ ideas and shared a little with the group. Weak (5 points) This manager was not good at communicating or listening to others’ ideas. This person shared little with the group and seemed more like a dictator than a communicator. Leadership Score: DELEGATION Excellent (10 points) This manager was excellent in delegating responsibilities to other group members and was clear with directions. This person followed up to make sure tasks were complete and did not try to do everything personally. Delegation Score: COMMUNICATION Excellent (10 points) This manager was excellent at communicating thoughts and processing information. This person also listened carefully to ideas from others and often shared information with the group. Communication Score: OVERALL SCORE: Judge: _______________________________________ Period: ________ Group Number: ______________ PERFORMANCE Excellent (10 points) This group appeared to be skilled, expressive and aware of the content. The intent and movement were authentic. No observable mistakes were made. Adequate (7points) This group appeared to be somewhat expressive and mostly aware of the content. The intent and movement was somewhat rehearsed. Some group members may have made some mistakes. Weak (5 points) This group appeared unrehearsed. The intent and movement was not realized. Many mistakes were made. Adequate (7 points) This group performed with some attention to quality of movement, body position and placement on stage. Some costume, props, and other details are noted. Weak (5 points) This group performed with little attention to quality of movement, body position and placement on stage. Little to no attention was placed on costumes, props, and other details. Adequate (7 points) This group had standard formations, some variety, some clear transitions, moderate pace, adequate transitions from section to section, and/or a somewhat segmented routine. Weak (5 points) This group had basic formations, little variety, scattered or slow transitions, bumping into other students during transitions, lacking creativity, and/or a very segmented routine Adequate (7 points) This group showed some creativity with movement, some costumes were evident, and some use of props. Weak (5 points) This group showed poor creativity in movement, costumes, props, and overall composition. Performance Score: EFFORT Excellent (10 points) This group performed with great attention to quality of movement, body position, placement on stage, costumes, props, and other details of dance. Effort Score: TRANSITIONS/FLOW Excellent (10 points) This group had exceptional precision in formations, unique transitional skills, fast/clean transitions, creative formations, patterns, and exceptional variety. Transitions/Flow Score: CREATIVITY Excellent (10 points) This group showed excellent creativity with movement, costumes, props, and overall composition. Creativity Score: OVERALL SCORE: