Supporting information – Tables
Table S1 Genotyping primers for mutants
Mutant line
Seed stock
Primer code
Primer sequences (5’-3’)
tt1
SALK_107737C
tt2-3
FLAG_106F02
tt2-5
SALK_005260
tt5-4
SALK_034145
tt6-1
SALK_061570
tt7-8
SALK_089186
tt8
SALK_063334
tt10-10
SALK_128292C
tt18-12
SALK_028793
tt19-1
SALK_113805C
fae1-2
SALK_086589
LP
RP
LP
RP
LP
RP
LP
RP
LP
RP
LP
RP
LP
RP
LP
RP
LP
RP
LP
RP
LP
RP
LBb1.3
AGCGATTGAATGTCTTGAAGC
AACCCTGCAATGACAAAGTTG
CTAAAACCGAATAACCCGACC
CAACAAGTGAAGTCTCGGAGC
GCGGGTCAGAATCTAGTTTCC
AAGCAGATGGTCGTTGATAGC
GGAGGGAATGTTTCTTCCTTG
TTTTTCTTTGATTTTCCGAAAG
ACTGCCAAGGTATGTGTTTCG
TACTCACGGTGGCCAGATAAG
ATGAATCAGACATCGCTCAGC
TGAACAAGTTAGGATGTCGGC
AACATTATTGCGACCGTTGAG
GGAAGAAGCATGGAACAGAAG
CAGAATCTGCTGATTTGGCTC
TCAGCCATTGTTTTTGGAAAC
TTCAACCATTTTGATACTTGCG
GTGTCTTCGTTTGCTAGCGAC
AGAGACTTGCCCAAAAGGTTC
CTTTCTGGAGCTGCACTCAAC
TTTGGCTGATGTAGGCTCATC
TTCGATGTGTGCAACAAGAAG
ATTTTGCCGATTTCGGAAC
All Salk lines
Table S2 Primer pairs used to amplify promoter and cDNA fragments for the
construction of ProLEC2:TT2 and ProABI5:TT2 molecular cassettes
Primer codes
Primer sequence(5'-3')
PCR product size
Hind III-ProABI5-F
CTCTAAGCTTCCTATGTAAAACATTCTCAGC
2140 bp
Kpn1-ProABI5-R
CCTAGGTACCAACAACTGCATCATATACACAAC
Hind III-ProLEC2-F
GGTGACAGTCAAGCTTATATTATC
Kpn 1-ProLEC2-R
TTACGGTACCTCCATTTTTCCCGGAGAGAGAGAG
Kpn 1TT2-F
TAACGGTACCATGGGAAAGAGAGCAACTACTAGTGTG
Sac 1-TT2-R
TAAAGAGCTCTCAACAAGTGAAGTCTCGGAGC
1330 bp
777 bp
Table S3 Ingredients in the buffers used for the CHIP enrichment test
Buffer type
MC buffer
Reaction buffer
IP buffer
High-salt IP buffer
LNDET buffer
Elution buffer
Ingredients
0.1 M sucrose, 50 mM NaCl , 0.1 M sucrose, 10 mM potassium
phosphate (pH 7.0)
150 mM NaCl, 1%Triton X-100, 1 mM EDTA, 0.1 % SDS,
150 mM NaCl, 1 mM EDTA, 1%Triton X-100, 0.1 % SDS,
50 mM HEPES (pH 7.5)
150 mM KCl, 10 mM ZnSO4, 0.05 % SDS, 5 mM MgCl2,
1 % Triton X-100,50 mM HEPES (pH 7.5)
500 mM KCl, 10 mM ZnSO4, 0.05 % SDS, 5 mM MgCl2,
1 % Triton X-100,50 mM HEPES (pH 7.5)
10 mM Tris, pH 8.0, 0.25 M LiCl, 1% deoxycholate,
1% Nonidet P-40, 1 mM EDTA
1% SDS, 10 mM EDTA, 50 mM Tris (pH 8.0)
Table S4 Primer pairs applied for the ChIP enrichment test
Code
Primer Sequence
FUS3-CHIP-F1
FUS3-CHIP-R1
FUS3-CHIP-F2
FUS3-CHIP-R2
FUS3-CHIP-F3
FUS3-CHIP-R3
FUS3-CHIP-F4
FUS3-CHIP-R4
FUS3-CHIP-F5
FUS3-CHIP-R5
FUS3-CHIP-F6
FUS3-CHIP-R6
FUS3-CHIP-F7
FUS3-CHIP-R7
FUS3-CHIP-F8
FUS3-CHIP-R8
FUS3-CHIP-F9
FUS3-CHIP-R9
FUS3-CHIP-F10
FUS3-CHIP-R10
FUS3-CHIP-F11
FUS3-CHIP-R11
EF1aA4-qRT-F
EF1aA4-qRT-R
TUB2-qRT-F
TUB2-qRT-R
AACTCCCGTTGGAGTTGGTC
CTTTGTGTCCTCCCGTGACA
GTGGAAAAGTGGACGTAGATGC
TTACTCATGGTCACGCCTCG
ATCGTGTGCGTGAGAAGTGT
AAATTGGCCGGAGCCACATA
AGCAAATCAAGATTTCGCGGT
TTTGGATTCCGGTCCACAGAA
TTCTGTGGACCGGAATCCAA
AACTTAGTACACACAACTTCAGGA
AGTCAATACATAAAATCTACA
AGCTGTAACAGATTTCAACTA
ACGCCCTTATCAAGAAAAATGGA
AACACATTTCCATGGGTTTTAACT
ACGGGCGTTACTAAATGAAAGT
TGCGACCAAGAATAAAGGCT
ACCATGCACAATCCACTCGT
ACGCAACAAGATCTAATGCCAC
AACCCAAATCTCTGATTGCCCA
AAATGGTGGATCTCTTTGGGTT
TGGACGGTTTTCACGTTTGG
TCCGTTATAGATTCGTACTAGCCA
CTGGAGGTTTTGAGGCTGGTA
CCAAGGGTGAAAGCAAGAAGA
ATCCGTGAAGAGTACCCAGAT
AAGAACCATGCACTCATCAGC