OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Agence nationale de sécurité sanitaire de l’alimentation, de
l’environnement et du travail (ANSES)/
French Agency for Food, Environmental & Occupational Health
Safety
Laboratoire de Santé Animale de Maisons-Alfort /
Maisons-Alfort Animal Health Laboratory
Unité Zoonoses Bactériennes /
Bacterial Zoonoses Unit
23, avenue du Général-de-Gaulle
94706 Maisons-Alfort Cedex
FRANCE
Tel.:
+ 33 (0)1 49 77 13 00
Fax:
+ 33 (0)1 49 77 13 44
e-mail address:
website:
Name of Head of Laboratory
(Responsible Official):
Name of OIE Reference Expert:
Annual reports of OIE Reference Centres, 2011
Brucellosis
bruno.garin-bastuji@anses.fr
www.anses.fr
Dr Pascal Boireau
Dr Bruno Garin-Bastuji
1
Brucellosis
Part I: Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease/topic at this laboratory
Test
For
Specificity
Total
RBT
6153
SAT
5518
CFT
6051
I-ELISA (bovine serum)
5450
I-ELISA (bovine milk)
Anti-smooth Brucella
I-ELISA (porcine serum)
102
858
Antibody
C-ELISA
23317
FPA
5442
MRT
101
B. ovis CFT
522
Anti-Brucella ovis
B. ovis I-ELISA
B. canis RSAT
3066
Anti-Brucella canis
102
Culture
Living bacteria
Brucella sp. culture / Isolation
123/24
Brucella identification and
biotyping a
Animal strains
Brucella species and biovars
identification
30
Human strains
25
Brucella ovis identification a
4
Brucella PCR
DNA
Brucella molecular typing
Brucella sp. detection
108
Brucella species, biovars, strains
(Bruce-ladder, PCR-RFLP, MLVA)
46
Control of RBT, MRT, CFT, SAT
antigens, ELISA & FPA kits
Batch control
According to EC regulations
and/or OIE requirements
35 batches b
Control of standard & control sera
Antibody
Anti-smooth Brucella
0
Control of Brucellin
Batch control
1c
Final lots control
4 Rev.1 batches
1 S19 batch
No RB51 batches
Control of Rev.1, S19 & RB51
anti-Brucella vaccines d
According to OIE Manual
No Rev.1 seed-lots
2 S19 seed-lots
Seed lots control
a
2.
from France, Spain and Sweden; b from France, Spain, United Kingdom (Great Britain); c from France; d from Spain.
Production and distribution of diagnostic reagents
Numbers supplied
Reagents
Nationally
To OIE Member Countries
National standard panel of sera
8
-
National RBT standard serum (BRU POS SE 01 eq. OIEISS)
4
-
National CFT/SAT/RT standard serum (BRU POS SE 02 eq. OIEISS)
3
-
National ELISA standard serum (BRU POS SE 03 eq. OIEELISAspSS)
13
3 (Sweden, South Korea)
National ELISA negative standard serum
-
-
Brucella reference & field strains
-
43 (Lithuania, Spain, UK North. Ireland)
Brucella reference and field strains DNAs
-
44 (Brazil, Spain, UK )
B. melitensis Rev.1 and S19 vaccine reference strains
-
4 B19 (Brazil) / 2 Rev.1 (Turkey)
B. abortus S99 antigen reference strain
-
-
Brucellaphages (Wb, Tb, Iz1 and R/C)
-
24 (Portugal, Thailand)
Anti-A, anti-M monospecific, anti-R and negative sera
-
26 (Germany, Portugal, UK North. Ireland)
57
70 (Ireland, Romania)
B. ovis antigen (CFT)
2
Annual reports of OIE Reference Centres, 2011
Brucellosis
Numbers supplied
Reagents
Nationally
To OIE Member Countries
B. ovis positive control serum (CFT)
16
6 (Romania)
B ovis negative control serum (CFT)
-
-
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
a)
Establishment and maintenance of a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organisation of regular inter-laboratory
proficiency testing to ensure comparability of results
 International inter-OIE reference laboratories FPA ring-trial (participant)
 International collaborative ring trial for the validation of the International Standard anti-B. suis serum
(participant)
b)
Organisation of inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results
 European ring-trial for the standardization of the CFT EU-SOP (including 25 EU NRLs - organizer).
 Collaborative European ring trial for validation of a Brucella suis EU candidate standard serum (including
25 EU NRLs - organizer).
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
See 2.
5.
Research and development of new procedures for diagnosis and control
- Diagnostic validation of a real-time PCR method for application on field animal samples (in progress).
- Comparison of CFT and iELISA for the diagnosis of B. ovis infection in sheep (Analysis – Manuscript
submitted).
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
- Report to the OIE questionnaire 2010 on wildlife diseases.
- 2010 Annual Report on Zoonoses to the European Food Safety Authority/European Centre for Disease
Prevention and Control.
- 2010 Annual Report to the French Ministry of Health on Brucella strains identified in humans in France.
- 2010 Annual Report to the French Ministry of Agriculture of the National Reference Laboratory for Animal
Brucellosis.
Annual reports of OIE Reference Centres, 2011
3
Brucellosis
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
This laboratory has been accredited by the French Committee for Accreditation (COFRAC) according to ISO/IEC
17025 standard as regards diagnostic tests for animal brucellosis (serology and bacteriology) as well as for the
Brucella identification and typing, the control of Brucella vaccines and the control of brucellosis diagnostic
antigens and ELISA kits according to OIE and EU standards [Accreditation n°1-2246 http://www.cofrac.fr/annexes/sect1/1-2246.pdf pages 16-19].
This laboratory has been inspected in 2009 and approved by the French Sanitary Authorities (AFSSAPS) for the
handling of Brucella according to official French regulations and WHO biosafety and biosecurity standards.
8.
Provision of consultant expertise to OIE or to OIE Member Countries
- ISO/IEC 17025 Assessment of the Portuguese Brucellosis NRL (LNIV), brucellosis laboratory activities, official
IPAC audit, Lisbon, Portugal, July 3-5, 2011.
- OIE Ad hoc Group on brucellosis meeting. Revision of the Terrestrial Code chapters on brucellosis (B. GarinBastuji, Chairman), OIE, Paris, 2011/07/20-22.
- ISO/IEC 17025 Assessment of the Belgian Brucellosis NRL (CERVA/CODA)), brucellosis laboratory activities,
internal audit, Brussels, Belgium, 2011/12/20.
- European Task Force on Monitoring Animal Disease Eradication - Bovine Brucellosis subgroup Meeting, Belfast
& Newry, UK Northern Ireland, 2011/12/05-08.
- Scientific Committee of the “Brucellosis 2011 International Conference”, Buenos Aires, Argentina, 2011/09/2123.
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
- 2010-2013 OIE Twinning programme with NIAH, Bangkok, Thailand
 1st consultancy mission to evaluate the progress in organisation, quality management activities and
implementation of serological tests as well as control of diagnostic reagents at NIAH, Bangkok, Thailand,
2011/04/11-15.
 2nd training session “Bacteriological and molecular detection, identification and typing of Brucella strains”
(2010-2013 OIE Twinning programme with NIAH, Bangkok, Thailand), ANSES, Maisons-Alfort, France,
2011/09/26-10/07 (3 trainees).
- One PhD student from Algeria (Algiers National Veterinary School), “Bacteriological and molecular detection,
identification and typing of Brucella strains”, 2010/12/27-2011/01/25 and 2011/07/11-08/08.
10. Provision of diagnostic testing facilities to other OIE Member Countries
- Control of Brucella vaccines and diagnostic reagents, Identification and typing of Brucella strains (see 1.)
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
- EU Brucellosis National Reference Laboratories 5th Annual Workshop organized by the EU-RL for Brucellosis
(Anses), Sliema, Malta, 2011/05/12-13.
12. Participation in international scientific collaborative studies
4
Annual reports of OIE Reference Centres, 2011
Brucellosis
- See 3. and,
- Collaborative work on the development of tests or testing strategies for the differentiation of B. suis-induced
antibodies from false positive serological results in porcine brucellosis ( “Improving diagnostics for safer
surveillance of porcine brucellosis” Club V project– AHVLA, United Kingdom; DTU, Denmark and Anses,
France – 2010-2011).
- Collaborative work on the MLVA of B. suis biovar 2 strains isolated from either domestic pigs, wild boars or
hares (Szent István University, Budapest, Hungary; CITA, Saragossa, Spain and Anses, France 2010-2012)
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

1.
2.
3.
4.
5.
6.
7.
8.
9.

1.
2.
Presentations at international conferences and meetings
Hendricks P, Garin-Bastuji B, Fediaevsky A, Dufour B, 2011. Use of the OASIS tool for the assessment of
exotic diseases surveillance system in France, example of bovine Brucellosis surveillance. International
Conference on Animal Health Surveillance (ICAHS) Lyon, France – 17-20/05/11 (Communication - abstract
published).
Albert D, Corde Y, Drapeau A, Durand B, Garin-Bastuji B, 2011. Preparation and validation of European
standard sera for the standardization of I-ELISA in sheep and goats. Brucellosis 2011, International Research
Conference Including the 64th Brucellosis Research Conference, Buenos Aires, Argentina, 21-23/09/11
(Poster).
Albert D, Cherchame E, Le Carrou G, Bounaadja LK, Garin-Bastuji B, 2011. Evaluation of a real-time PCR
targeting IS711, BCSP31 & per gene for a rapid diagnosis of brucellosis on animal samples. Brucellosis
2011, International Research Conference Including the 64 th Brucellosis Research Conference, Buenos Aires,
Argentina, 21-23/09/11 (Communication).
Corde Y, Drapeau A, Albert D, Garin-Bastuji B, 2011. Assessment of a lateral flow immunochromatographic assay for the serological diagnosis of canine brucellosis due to Brucella canis. Brucellosis
2011, International Research Conference Including the 64 th Brucellosis Research Conference, Buenos Aires,
Argentina, 21-23/09/11 (Poster).
Ekgatat M, Tiensin T, Kanitpun R, Buamithup N, Jenpanich C, Warrasuth N, Wongkasemjit S, Garin-Bastuji
B Jirathanawat V, 2011. Brucellosis control and eradication programme in Thailand - Preliminary evaluation
of the epidemiological situation in cattle, buffaloes and sheep and goats. Brucellosis 2011, International
Research Conference Including the 64th Brucellosis Research Conference, Buenos Aires, Argentina, 2123/09/11 (Poster).
Praud A, Meyer L, Garin-Bastuji B, 2011. Estimation of sensitivity and specificity of nine serological tests
for the diagnosis of porcine brucellosis. Brucellosis 2011, International Research Conference Including the
64th Brucellosis Research Conference, Buenos Aires, Argentina, 21-23/09/11 (Poster).
Praud A, Champion JL, Meyer L, Garin-Bastuji B, 2011. Preliminary study towards the validation of an
alternative serological test for the diagnosis of Brucella ovis infection in sheep. Brucellosis 2011
International Research Conference Including the 64 th Brucellosis Research Conference, Buenos Aires,
Argentina, 21-23/09/11 (Poster).
Lounes N, Bouyoucef A, Garin-Bastuji B, 2011. Bovine and caprine brucellosis in the Central Region of
Algeria. Brucellosis 2011, International Research Conference Including the 64 th Brucellosis Research
Conference, Buenos Aires, Argentina, 21-23/09/11 (Poster).
Rahal K, Bennadji A, Dahmani A, Dechicha A, Khaled H, Merdja H, Lounes N, Rousset E, Sidi Boumedine
K, Thiery R, Laroucau K, Garin-Bastuji B, Bouyoucef A, 2011. Séro-prévalence apparente de la brucellose,
chlamydiose et fièvre Q chez les ovins de la région de Ksar Boukhari. 4èmes journées vétérinaires de Blida,
Blida, Algeria, 2011/11/28-29 (Communication).
Scientific publications in peer-reviewed journals
Antras V, Garin-Bastuji B, 2011. La brucellose porcine en Polynésie française. Bull. Epidémiol. Santé Anim.
Alim., 43: 35-38.
Bronner A, Ledru M, Garin-Bastuji B, 2011. Étude de cas sur les foyers de brucellose porcine à Brucella suis
biovar 2 en France métropolitaine entre 1993 et 2008. Bull. Epidémiol. Santé Anim. Alim., 42: 6-10.
Annual reports of OIE Reference Centres, 2011
5
Brucellosis
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
Bronner A, Marcé C, Fradin N, Darrouy-Pau C, Garin-Bastuji B, 2011. Bilan de la surveillance de la
Brucellose porcine en France en 2010 : détection de foyers chez des porcs de race locale. Bull. Epidémiol.
Santé Anim. Alim., 46/Spécial MRC – Bilan 2010, 39-40.
Fediaevsky A, Dufour B, Garin-Bastuji B, 2011. Maintenir la vigilance contre la brucellose bovine en France
en 2010. Bull. Epidémiol. Santé Anim. Alim., 46/Spécial MRC – Bilan 2010, 10-14.
Fediaevsky A, Garin-Bastuji B, Dufour B, 2011. Aucun foyer de brucellose ovine et caprine détecté en
France en 2010. Bull. Epidémiol. Santé Anim. Alim., 46/Spécial MRC – Bilan 2010, 32-35.
Garin-Bastuji B, Durand B, Hautier JP, Campos V, 2011. La brucellose porcine à Wallis et Futuna. Bull.
Epidémiol. Santé Anim. Alim., 43: 31-34.
Garin-Bastuji B, Fediaevsky A, 2011. Un foyer de brucellose bovine en Belgique ou l’importance de la
surveillance en territoire officiellement indemne. Bull. Epidémiol. Santé Anim. Alim., 42: 10
Hendricks P, Garin-Bastuji B, Fediaevsky A, Dufour B, 2011. Use of the OASIS tool for the assessment of
exotic diseases surveillance system in France, example of bovine Brucellosis surveillance, Prev. Vet. Med.,
Accepted
López-Goñi I, García-Yoldi D, Marín CM, de Miguel MJ, Barquero-Calvo E, Guzmán-Verri C, Albert D,
Garin-Bastuji B, 2011. New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the
discrimination of Brucella suis and Brucella canis, Vet. Microbiol. 154:152-155.
McGiven J, Taylor A, Duncombe L, Sayers R, Albert D, Banai M, Blasco JM, Elena S, Fretin D,
Garin-Bastuj B, Melzer F, Muñoz PM, Nielsen K, Nicola A, Scacchia M, Tittarelli M, Travassos Dias I,
Walravens K, Stack J, 2011. The First International Standard Anti-Brucella melitensis Serum. Rev. Sci. Tech.
OIE, 30(3): In press.
Payne A, Rossi S, Lacour SA, Vallée I, Garin-Bastuji B, Simon G, Hervé S, Pavio N, Richomme C, Dunoyer
C, Bronner A, Hars J, 2011. Bilan sanitaire du sanglier vis-à-vis de la trichinellose, de la maladie d’Aujeszky,
de la brucellose, de l’hépatite E et des virus influenza porcins en France. Bull. Epidémiol. Santé Anim. Alim.,
44: 2-8.
Praud A, Gimenez O, Zanella G, Dufour B, Pozzi N, Antras V, Meyer L, Garin-Bastuji B, 2011. Estimation
of sensitivity and specificity of five serological tests for the diagnosis of porcine brucellosis, Prev. Vet. Med.,
In press.
Praud A, Champion JL, Corde Y, Drapeau A, Meyer L, Garin-Bastuji B, 2011. Assessment of the diagnostic
sensitivity and specificity of an indirect ELISA kit for the diagnosis of Brucella ovis infection in rams. BMC Vet. Res.,
Accepted.

Other communications
o
Book chapters:
1.
2.
3.
Cutler SJ, Zygmunt MS, Garin-Bastuji B, 2012. Brucella species: Brucellosis. In: M Elschner, S Cutler, M
Weidmann & P Butaye Eds, BSL3 and BSL4 Agents: Epidemiology, Microbiology, and Practical Guidelines,
1st Edition, Wiley-VCH Verlag GmbH & Co. KGaA, 19-36, In press.
Olsen SC, Garin-Bastuji B, Blasco JM, Nicola AM, Samartino L, 2012. Swine Brucellosis, In: BE Straw, JJ
Zimmerman, S D’Allaire, DJ Taylor Eds, Diseases of Swine, 10th Edition, Blackwell, USA, In press.
Garin-Bastuji B, 2011. Brucella spp., In: Fuquay JW, Fox PF and McSweeney PLH (eds.) Encyclopaedia of
Dairy Sciences, 2nd Edition, Vol. 4, Academic Press, San Diego, 31-39.
o
PhD Thesis:Nil
o
Consultancy and expertise reports
Collective, 2011. European Task Force for Monitoring Disease Eradication in the Member States, Report of the
meeting of the Bovine Brucellosis subgroups of the Task Force on monitoring animal disease eradication held in
Santander, Spain, 27-28/10/10, SANCO/10114/2011, 15 pp, 01/11.
_______________
6
Annual reports of OIE Reference Centres, 2011