Stable Cell Line Construction
Quotation Request Form
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Page 1 of 2
Stable cell line construction service
For the development of stable cell lines for protein expression and purification.
Customer Information
If you have an existing account with ProteinCT, just fill in your name and email address or your Account No.
Name of Requestor:
Account No.:
Name of PI:
First:
Last:
Middle:
Title:
Organization:
Phone:
Fax:
Email Address:
Shipping Address
Project Information
Nature of Inquiry
Pricing estimation
Quote for ordering
Estimated Project
Initiation
Immediately
>6 months
1-3 months
3-6 months
Other, please specify
Research Use Only
Clinical Applications
Intended Use
For grant application purpose
Please note that we produce materials for research use only. We can perform developmental work for future
clinical applications, but are not GMP-certified.
GLP compliance
Yes
Application for
stable cell lines
Protein Purification & Production (this service is designed for application in protein production)
Other, please specify
Application for
produced proteins
No
Research reagent
Cell-based Assay
Other, please specify
Animal Toxicity Study
Quotation request Checklist
Please check before submitting the quotation request form, thank you.
Gene Synthesis and vector
construction
Subcloning with template and target
vectors supplied by user
Gene sequence or accession number
Gene sequence or accession
number
Desired vector elements:
e.g., promoter, 5’/3’ UTR, tags,
selection marker, etc.
Vector sequences and vector maps (e.g. NTI
file)
User supplied ready-to-use
expression vector
Gene sequence or accession number
Vector sequences and vector maps (e.g.
NTI file)
> 5µg of template plasmid DNA
> 5µg of expression plasmid DNA
> 5µg of target plasmid DNA
Available expression protocol
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Stable Cell Line Construction
Quotation Request Form
Page 2 of 3
Service Specifications
Please fill in project requirement details.
1. Target Protein Information
Protein name:
Accession number:
Species:
MW:
Target: Protein sequence:
Please indicate mutations/variants as compared to the sequence corresponding to the
accession number.
Membrane-bound
Secreted
Protease
Other enzymes
Cytosolic
IgG antibody
Nuclear
Periplasmic
Toxic to the cell
Target properties:
Other features of the native protein to consider, please specify:
e.g., oligomer state, cofactors, posttranslational modifications/processing
Has the protein been expressed previously, including both transient and/or stable
expression?
Yes
No
If YES, please attach a word document with expression details including vector information,
expression cell line, expression conditions, expression level, purification method, yield and
Expression system: other related details, so we can provide a better evaluation for your project.
Select a host cell line for stable cell line construction:
HEK293
CHO
Other (Please specify):
No preference
HEK293 or CHO or their derivatives will be used as default cell line if no preference is
selected.
Secretion signal
sequence:
Preferred secretion signal sequence:
Its own signal peptide
No preference
Other (Please specify):
N-terminal
C-terminal
No
Purification tag: Please specify name and sequence of purification tag for target protein :
Please specify protease cleavage site and sequence for tag cleavage:
Comments:
Please complete the form and submit to order@proteinct.com.
Our service representative will contact you shortly with a quote.
Stable Cell Line Construction
Quotation Request Form
Page 3 of 3
Service Specifications
Please fill in project requirement details.
2. Expression Construct Generation
Gene Synthesis and vector construction by ProteinCT:
Codon optimization
Yes
No
Preferred elements, e.g. promoter, 5’/3’UTR, selection marker, etc.:
Enter target gene sequence:
Subclone from User supplied template DNA and target vector
Minimum 5µg of plasmid DNAs are required for this operation:
Template vector name:
Selection marker:
Target vector name:
Selection marker:
Please attach vector maps (e.g. NTI file)
Starting material:
Enter your gene sequence:
User supplied expression ready construct
Minimum 5µg of plasmid DNAs are required for this operation:
Expression vector name:
Selection marker:
Please attach vector map (e.g. NTI file)
Enter vector sequence:
Other (Please specify):
3. Stable Cell Line Construction
Plasmid purification Endotoxin free plasmid DNA will be prepared for stable cell line construction
Screening method:
Western Blot
Flow Cyt
ELISA
Other, please specify
ELISA and/or related immunoassay is the default detection method
Desired expression Please specify expression level:
level: Desired purification yield:
will help us better evaluate and design your project.
Preferred assay for This
Please select all that may apply
expression detection
SDS-PAGE
IEF
ELISA
HPLC
and characterization:
Other, please specify
Special For example, buffers, handling, storage, etc.
requirements: Please specify
Other comments:
Purity
Endotoxin