OIE Collaborating Centre Reports
Activities in 2013
Title of Collaborating Centre:
Address of Collaborating Centre:
OIE Collaborating Centre for
Zoonoses of Asia-Pacific
No 427 Maduan Street Nangang District Harbin,
P. R. China
Tel.:
+86 18946066220
Fax:
+86-451-51997166
e-mail address:
website:
zj@hvri.ac.cn
www.hvri.ac.cn
Name of Director of Institute
(Responsible Official):
Kong Xiangang
Name (including Title and
Position) of Head of the
Collaborating Centre (formally
OIE Contact Point):
Wang Xiaomei
Name (including Title and
Position) of writer of this report
(if different from above)
Zhang Jing
Annual reports of OIE Reference Centres, 2013
1
Zoonoses of Asia-Pacific
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
ToR:
To provide services to the OIE, in particular within the region, in the designated
specialty, in support of the implementation of OIE policies and, where required, seek for
collaboration with OIE Reference Laboratories
ToR:
To identify and maintain existing expertise, in particular within its region
1.
Activities as a centre of research, expertise, standardisation and dissemination of
techniques within the remit of the mandate given by the OIE
Disease control
Epidemiology, surveillance, risk assessment, modelling
Training, capacity building
Zoonoses
Wildlife
Avian diseases
Aquatic animal diseases
Animal welfare
Diagnosis, biotechnology and laboratory
Veterinary medicinal products
Vaccines
Food safety
Feed safety
Other (Name the category:
)
DIAGNOSIS, BIOTECHNOLOGY AND LABORATORY
Title of activity
Scope
Diagnostic activities for
Tuberculosis
A total of 1593 Bovine serum and 581 nasal swabs and Oesophagus
Pgayngeal (OP) from Northeast area and Xinjiang Province of China
were analysed using ELISA antibody test kit desinged by our Center.
Diagnostic atctivates for
animal influenza
A total of 40000 swabs and 20000 poultry or swine sera were
detected and analysed for animal influeza surveillance throuth virus
isolation or HI test in China.
Diagnostic activities for
brucellosis
A cELISA kit for detecting antibodies of smooth brucella was
registered in China,and about 1000 sera from immunized and 3000
nonimmunized ovine and bovine were analyzed for the presence of
antibody for the surveillance activity in China 2013.
Diagnostic activities for
equine influenza
A total of 1306 equine serum samples collected from different places
in China were ananlysed for the presence of anti-EIV antibody
through HI test.
VACCINES
2
Title of activity
Scope
NDV based live attenuated
vaccine candidates
development for Zaire
Ebola, Lassa fever and
Marburg hemorrhagic
fever.
By using an NDV LaSota virus reverse genetics system, we
constructed the recombinant NDV vectored vaccines expressing
ZEBOV GP,MARV GP and LASV GP protein, and evaluated their safety
and immunoginicity in mice and rhesus macaques.
Annual reports of OIE Reference Centres, 2013
Zoonoses of Asia-Pacific
Recombinant adenovirus
vaccine of West Nile Virus.
Constructed a recombinant adenovirus co-expressing NS1 and E
protein of West Nile Virus using Food-and-Mouth disease virus
(FMDV) 2A peptide was as a linker. IFA and West blotting results
showed that E and NS1 proteins were expressed correctly, and self-c
Vaccines for Avian
influenza
The avian influenza DNA vaccine（H5 Subtype, pH5-GD）,the
recombinant DEV vector-based live vaccine, the recombinant HVT
vector-based live vaccine were under development.
Getetic manipulation for
Salmonella enterica
serovar Enteritidis
A novel attenuated vaccine has been constructed by deleting four
genes of Salmonella enterica serovar Enteritidis, the LD50 of this
strain exhibited a greater than 2×103-fold attenuation compared
with that of the wild-type,
OTHER ACTIVITY 1
Title of activity
Scope
Quality Assurance
GLP and ISO 17025
ToR:
To propose or develop methods and procedures that facilitate harmonisation of
international standards and guidelines applicable to the designated specialty
2.
Proposal or development of any procedure that will facilitate harmonisation of
international regulations applicable to the surveillance and control of animal diseases,
food safety or animal welfare
Proposal title
Management of
Equine influenza
Scope/Content
Applicable area
This study was done in full
collaboration with Irish Equine
Center, the OIE equine influenza
Reference Laboratory.
Surveillance and control of animal diseases
Food safety
Animal welfare
ToR:
To establish and maintain a network with other OIE Collaborating Centres designated
for the same specialty, and should the need arise, with Collaborating Centres in other
disciplines
ToR:
To carry out and/or coordinate scientific and technical studies in collaboration with
other centres, laboratories or organisations
3.
Did your Collaborating Centre maintain a network with other OIE Collaborating Centres
(CC), Reference Laboratories (RL), or organisations designated for the same specialty, to
coordinate scientific and technical studies?
Yes
Name of OIE CC/RL/other
organisation(s)
ELISA and Molecular
Techniques in Animal
Disease Diagnosis, FAO/IAEA
Animal Production and
Health Laboratory.
No
Location
Vienna
Annual reports of OIE Reference Centres, 2013
Region of
networking Centre
Africa
Americas
Asia and Pacific
Europe
Middle East
Purpose
To apply for the FP7 project:Linking
Epidemiology and Laboratory Research
on Transboundary Animal Diseases and
zoonoses in China and EU
3
Zoonoses of Asia-Pacific
Name of OIE CC/RL/other
organisation(s)
Location
the OIE cc- of Biotechnologybased Diagnosis of Infectious
Diseases in Veterinary
Medicine National
Veterinary Institute, Sweden.
Sweden
Food and Agriculture
Organization of the United
Nations (FAO)
Italy
with Irish Equine Center, the
OIE equine influenza
Reference Laboratory.
Irish
Region of
networking Centre
Africa
Americas
Asia and Pacific
Europe
Middle East
To apply for the FP7 project:Linking
Epidemiology and Laboratory Research
on Transboundary Animal Diseases and
zoonoses in China and EU.
Africa
Americas
Asia and Pacific
Europe
Middle East
conducting genetic sequence analysis of
the swine influenza virus and field
sampling and laboratory teseting to
characterize viruses in the livestock
sector
Africa
Americas
Asia and Pacific
Europe
A total of 20 equine nasal swabs 43
equine serum samples were imported to
HVRI from Irish equine center in use of
comparison test.
Middle East
4.
Purpose
Did your Collaborating Centre maintain a network with other OIE Collaborating Centres,
Reference laboratories, or organisations in other disciplines, to coordinate scientific and
technical studies?
Yes
Name of OIE CC/RL/other
organisation(s)
Bill & Melinda Gates
Foundation
No
Location
Region of
networking Centre
United
States of
America
Africa
Americas
Asia and Pacific
Europe
Middle East
Purpose
To apply effective vaccine to control
Contagious bovine pleuropneumonia in
African countries.
ToR:
To place expert consultants at the disposal of the OIE.
5.
Did your Collaborating Centre place expert consultants at the disposal of the OIE?
Yes
4
No
Name of expert
Kind of consultancy
Subject
Dr Chen Hualan
Conselor
To update the the Terrestrial Manual
Dr Jia Honglin
Conselor
By attending Asia pacific workshop on Multi
collaboration for the prevention and control
of Zoonoses
Dr Wang Xiaojun
Conselor
OIE Expert Surveillance Panel on Equine
Influenza Vaccine Composition, Conclusions
and Recommendations
Dr Chen Hualan
Conselor
H7N9 technical and programmatic meeting,
Annual reports of OIE Reference Centres, 2013
Zoonoses of Asia-Pacific
ToR:
To provide, within the designated specialty, scientific and technical training to personnel
from OIE Member Countries
6.
Did your Collaborating Centre provide scientific and technical training, within the remit of
the mandate given by the OIE, to personnel from OIE Member Countries?
Yes
No
If the answer is yes, please provide the total number of trained persons for each of the following
categories:
a)
Technical visits: ................................................................
b)
Seminars: .........................................................................
c)
Hands-on training courses: .............................................. 2
d)
Internships (>1 month): ...................................................
Type of technical
training provided
(a, b, c or d)
c
Content
Country of origin of the
expert(s) provided with
training
No. participants from
the corresponding
country
Egypt
2
Providing training on diagnosis of
animal infectious disease
ToR:
To organise and participate in scientific meetings and other activities on behalf of the
OIE
7.
Did your Collaborating Centre organise or participate in the organisation of scientific
meetings on behalf of the OIE?
Yes
No
National/
International
Title of event
Co-organiser
Date
(mm/yy)
Location
No.
Participants
International
OFFLU conferece in
Beijing
OIE, FAO
December,
2013
Beijing
42
International
Opening Ceremony of
the FAO Reference
Centre for Animal
Influenza and Technical
Workshop
FAO,
MoA,P.R.China
March,2013
Harbin
100
International
Sino-British symposium
on avian infectious
diseases
The Pirbright
Institute
July, 2013
Harbin
50
OIE
March, 2013
Paris
13
OIE
November,
2013
Kathmand
u
100
HVRI
April, 2013
Yang Zhou
300
International
International
National
2013 equine influenca
ESP meeting
Asia Pacific Workshop on
Multisectoral
Collaboration for the
Prevention and Control
of Zoonoses
Avian infectious disease
Annual reports of OIE Reference Centres, 2013
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Zoonoses of Asia-Pacific
ToR:
To collect, process, analyse, publish and disseminate data and information relevant to
the designated specialty
8.
Publication and dissemination of any information within the remit of the mandate given by
the OIE that may be useful to Member Countries of the OIE
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 13
Qianyi Zhang,* Jianzhong Shi,* Guohua Deng,* Jing Guo,* Xianying Zeng,* Xijun He，et al.. H7N9 Influenza
Viruses Are Transmissible in Ferrets by Respiratory Droplet. Science, 2013, 18 July 2013 / Page 3/
10.1126/science.1240532.
Ying Zhang*, et al.. H5N1 Hybrid Viruses Bearing 2009/H1N1 Virus Genes Transmit in Guinea Pigs by
Respiratory Droplet. Science, 2013, 340(6139):1459-63.
Deng Guohua*, et al., Complex reassortment of multiple subtypes of avian influenza viruses in domestic ducks
at the Dongting Lake Region of China. Journal of virology, 2013., 87(17):9452-62.
Xianglian Yuan*,et al.,Characterization of rv0394c gene encoding hyaluronidase and chondrosulphatase
activity from Mycobacterium tuberculosis H37Rv,Tuberculosis (Edinb). 2013 May;93(3):296-300.
Wanjiang, Zhang et al. Characterization of the IncA/C plasmid pSCEC2 from Escherichia coli of swine origin
that harbours the multiresistance gene cfr. J Antimicrob Chemother. 2014 Feb;69(2):385-9.
Zhiyuan Wen et al.. Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells
independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry. Virol J.
2013 Nov 9;10(1):331.
Enchen Sun, et al., Antibodies generated by immunization with the NS1 protein of West Nile virus confer
partial protection against lethal Japanese encephalitis virus challenge. Vet Microbiol. 2013 Sep 27;166(12):145-53.
Ronghong Hua, et al., Comprehensive Mapping Antigenic Epitopes of NS1 Protein of Japanese Encephalitis
Virus with Monoclonal Antibodies. PLoS One. 2013 Jun 18;8(6):e67553.
Fangkun Wang, et al. Comparison of genomes of Brucella melitensis M28 and the B. melitensis M5-90
derivative vaccine strain highlights the translation elongation factor Tu gene tuf2 as an attenuation-related
gene. Infect Immun. 2013 Aug;81(8):2812-8.
Junping Li*, Yongping Jiang,Shuangcheng Zhao, Xiaofei Chang, Jinxiong Liu, Xianying Zeng, Yanbing Li, and
Hualan Chen. Protective Efficacy of an H5N1 DNA Vaccine Against Challenge with a Lethal H5N1Virus in Quail.
Avian Disease.2013, 56:937-939.
Jianzhong Shi, et al.. Isolation and characterization of H7N9 viruses from live poultry markets—Implication of
the source of current H7N9 infection in humans. Chinese Science Bulletin, 2013, 2013,10.1007/s11434-0135873-4.
Junping Li*, Yongping Jiang,Shuangcheng Zhao, Xiaofei Chang, Jinxiong Liu, Xianying Zeng, Yanbing Li, and
Hualan Chen. Protective Efficacy of an H5N1 DNA Vaccine Against Challenge with a Lethal H5N1Virus in Quail.
Avian Disease.2013, 56:937-939.
Jinxiong Liu*, et al. Recombinant duck enteritis virus works as a single-dose vaccine in broilers providing rapid
protection. Antiviral Research, 2013, 97:329-333.
b)
International conference presentations: ......................... 6
Dr Chen Hualan, Molecular Factors Contribute to the Transmission of Influenza Viruses. Asian-African Research
Forum on Emerging and Reemerging Infectious, Tokyo,Japan
Dr Li Chengjun, HVRI: Brief Introduction, Successes in Animal Influenza Research and Control. Opening
Ceremony of the FAO Reference Centre for Animal Influenza and Technical Worksho Harbin, China
6
Annual reports of OIE Reference Centres, 2013
Zoonoses of Asia-Pacific
Dr Chen Hualan, H7N9 technical and programmatic meeting., Animal findings and animal experiments in China
Rome, Italy
Dr Liu Jinxiong, Control of H5N1 avian influenza, Sino-British symposium on avian infectious diseases control
and prevention, Sino-British symposium on avian infectious diseases control and prevention. Harbin, China
Dr. Honglin Jia, East Asia symposium on farm biosecurity., The outbreak of Important epidemics in pig farms
and chicken farms and the present situation of farm biosecurity in China, Osaka, Japan.
Dr. Honglin Jia, Asia-Pacific workshop on multi-sectoral collaboration for the prevention and control of
zoonoses., Zoonosis in the wildlife of China, Nepal, China
c)
National conference presentations: ................................ 3
Dr Bu Zhigao, The strategies and techniques for prevention and control of brucellosis. Chinese science and
technology conference on zoonosis, Changchun, China.
Dr Liu Siguo, The epidemiology and dissemination mechanism of the multi-resistant gene cfr in Staphylococci
and Eescherichia coli from swineChinese science and technology conference on zoonosis, Changchun, China
Dr Wang Chunlai, The preparation technology of bacterial ghost vaccine. Conference on the development of
animal vaccine, Tai Zhou , China
d)
Other: ...............................................................................
(Provide website address or link to appropriate information)
_______________
Annual reports of OIE Reference Centres, 2013
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