Faculty of Science
Microbiological Risk
Assessment
Where space on this form is insufficient attach a separate sheet and indicate in appropriate place.
This form is to be filled in by the subject coordinator in consultation with the laboratory manager. Please refer to
AS/NZS 2243.3:2002 Safety in Laboratories Part 3: Microbiological aspects and containment facilities.
If the work involves organisms of Risk Group 2 the Laboratory Manager will seek approval from the Biological
Safety Committee (BSC) prior to commencement.
Micro-organism
Group
Name of Micro-organism
Bacteria
Chlamydiae
Rickettsiae
Mycoplasma
Parasite
Fungi
Virus
Prion
Risk Group
Special Precautions
(AS/NZS2243.3)
(refer to AS/NZS 2243.3)
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Do the micro-organisms produce infection in humans?
yes
no
If yes, please specify:
Are the micro-organisms highly infectious to humans?
yes
no
yes
no
What level of containment is required?
PC1
PC2
Are containment levels adequate?
yes
no
Does the Biosafety Committee carry out an annual inspection of the laboratory?
yes
no
Did the facility pass the annual inspection by the IBSC?
yes
no
Will the micro-organisms be stored in the facility?
yes
no
If yes, specify:
Is immunisation required when working with these micro-organisms?
If yes, specify the requirements:
Containment Facilities
If yes, when was the last inspection of the laboratory carried out by the IBSC?
If yes, specify the storage requirements:
Fridge
Freezer
Cold room
Incubator
Liquid Nitrogen
bench
Other: (please specify)
Is there proper labelling of stored organisms?
Microbiological Risk Assessment form V1
yes
no
Page 1
Procedures & Risk Controls
What procedures will be used with the micro-organisms? (Please tick appropriate box)
Aspiration
Sonication
Slide Preparation
Vigorous Shaking/Mixing
Pouring
Pipetting
Blending
Grinding
Centrifugation
Using Automated Equipment
Microbiological/Tissue Culture
Other:
Are there written procedures in case of accidents and/or spillage?
yes
no
Is there appropriate equipment and supplies to cater for accidents and/or spills?
yes
no
Are there adequate washing facilities available for use?
yes
no
yes
no
yes
no
yes
no
Are there appropriate sterilisation/autoclave facilities available?
N/A
Are procedures for cleaning or laundering of protective clothing adequate?
Is disposal/treatment of infectious material appropriate?
List any specific disposal procedures:
N/A
Proposed Precautions and/or Controls
List the protective devices intended to be used, including Personal Protective Equipment (PPE):
Biological Safety Cabinet I
Gown
Disinfectant
Biological Safety Cabinet II
Face Mask
Sharps Disposal
Gloves
Face Shield
Other:
N/A
Is the Biological Safety Cabinet NATA certified annually?
yes
no
Have the following factors been adequately considered?
Safety Training
yes
no
Legislative Standards
yes
no
Job Specific Training
yes
no
Supervision
yes
no
Other (please specify):
Special Approvals:
Has approval been obtained from the following? Please attach as appropriate.
Is approval required?
Are documents attached?
Institutional Biosafety Committee (IBSC)
yes
no
yes
no
Office of the Gene Technology Regulator (OGTR)
yes
no
yes
no
Australian Quarantine Inspection Service (AQIS)
yes
no
yes
no
Other:
yes
no
yes
no
Comments:
Microbiological Risk Assessment form V1
Page 2
Actions / Comments:
Remaining Risks:
List of Attachments:
1
2
3
4
5
6
Risk assessment completed by:
Name:
Signature:
Date:
Name:
Signature:
Date:
Name:
Signature:
Date:
Name:
Signature:
Date:
Name:
Signature:
Date:
Consultation
Signed (ALL persons involved in the project)
Approvals and Comments:
Name
Signature
Approval
y/n
Facility Manager
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Head of School
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Microbial Risk Assessment
Page 3 of 3
Comments