Characteristics of the Onchocerca volvulus molting L3 cDNA Library
(Ov3M)
Vector: Lambda Uni-ZAP XR (Lambda ZAP cut with EcoRI and XhoI - Stratagene)
Insert: Onchocerca volvulus L3/L4 transition cDNA.
Cloning Sites: EcoRI (5' end) and XhoI (3' end)
Unamplified Titer: 2.2 X 10 6 pfu/ml
Amplified Undiluted Titer: ~ 10 10 pfu/ml
Average Insert Size: ~ 1100 bp
Background: 7% non-recombinants
Host Strain: XL-1 Blue MRF' strain
Host Strain Information:
The XL-1 Blue MRF' strain is the recommended host strain for amplification and
screening of this Lambda Uni-ZAP cDNA library. The XL-1 Blue MRF' strain is
restriction minus and is recommended for plating unamplified libraries, and for
amplifying and screening libraries. It also enables blue/clear color selection of
recombinant clones when grown on plates containing IPTG and X-gal.
The bacterial stock is stored and streaked on LB-tet plates and grown as overnight
cultures in the absence of tetracycline.
Strain Genotype:
(mcrA) 183, (mcrCB-hsdSMR-mrr) 173, end A1, supE44, thi-1, recA1, gyrA96, relA1,
lac [F' proAB, lac IqZ M15, Tn10 (tetr)].
Reference: Jerpseth, B., et al, (1992) Strategies 5: 81-83.
Upon Receiving the Library:
The library is frozen in 7% DMSO. Thaw in hand quickly and dilute 1:10 in SM buffer
immediately. Add chloroform to 0.3%, mix, and store at 4oC.
Acknowledgements:
Michelle Lizotte-Waniewski and Dr. Steven A. Williams
Department of Biological Sciences
Clark Science Center
Smith College
Northampton, MA 01063
Dr. Sara Lustigman
New York Blood Center
310 East 67th Street
New York, NY 10021
Tel: (413) 585-3826
Tel: (212) 570-3119
FAX: (413) 585-3786
FAX: (212) 570-3180