Instruments and Methods
Optical Instruments
CTD: SBE 19, measures conductivity, temperature, and depth
AC-9: provide in situ beam attenuation and absorption coefficients from 400 nm to 720 nm.
OCR- 200 (Ed), OCR-200 (Lu): Satlantic multlispectral radiometer, irradiance and radiance
sensors
HS-6: multi-spectral backcattering sensor, is typically measured in terms of the Volume
Scattering Function (ß) averaged over a range of angles, which then may be used to estimate
the backscattering coefficient (bb). bb wavelengths: 442, 470, 510, 589, 620, and 675 nm
Methods
Total Suspended Material (TSM) :
Strickland J.D.H. and Parsons T.R. (1972) IV.2.1. Weight of Microscopic Material. A
Practical Handbook of Seawater Analysis. Bulletin 167, Second Edition.
Colored Dissolved Organic Matter (CDOM):
Materials Preparation:
Ciotti A.M., Bricaud A. (2006) Retrievals of a size parameter for phytoplankton
and spectral light absorption by colored detrital matter from water-leaving radiances at
SeaWiFS channels in a continental shelf region off Brazil Limnol. Oceanogr.: Methods 4, 237–
253
Samples Analysis:
Miller R.L., Belz M., Del Castillo C, Trzska R. (2002) Determining CDOM Absorption
Spectra in Diverse Coastal Environments Using a Multiple Pathlength, Liquid Core Waveguide
System. Continental Shelf Research Vol. 22 Issue: 9 Pages: 1301-1310
Ortiz Rosa S. (2010) Optical Properties and Photochemical Response of Colored
Dissolved Organic Matter (CDOM) at Jobos Bay National Estuarine Research Reserve
(JOBANERR). Thesis MS, University of Puerto Rico, Mayaguez, PR.
Chlorophyll a (Chla):
Samples Preparation:
The filters were placed in 15mL centrifuge tube and soaked with 10 mL of 90% acetone.
Tubes were covered with aluminum foil to protect from light. Samples were extracted
overnight at 5ºC. After the overnight extraction the contents in the tube were transferred to a
glass grinding tube and mechanically grinded.
Samples Analysis:
Parksons, T. R.,Y. Maita,C. M. Lalli. (1984) 4.3 Fluorometric Determination of
Chlorophylls. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon
Press.