SOP Number: CP018.00
Title: Preparation of the Humidifier Stand
Revision No: Replaces:
00
N/A
Author: Tatiana Zanganeh
I
Date in effect: Page:
7/15/2010
Page 1 of 4
MAL Director: Dr. Peggy Borum
FSHN Chair: Dr. Neil Shay
PURPOSE
The purpose of the humidifier is to maintain temperature and humidity in the incubator at a level
that is comfortable for the piglet.
II
SCOPE
This procedure instructs how to prepare a humidifier stand so that the piglets are kept in an
environment with appropriate temperature and humidity regulation.
III
RESPONSIBILITIES
It is the responsibility of Metabolic Assessment Laboratory personnel to follow this procedure.
It is the responsibility of supervisory personnel to ensure compliance with this procedure and to
train employees and students responsible for performing this procedure. Students will report
accidents to the principal investigators immediately.
IV
REFERENCES
N/A
V
REAGENTS AND MATERIALS
V.A.
V.B.
VI
Disinfectant cleaning solution
Deionized (DI), distilled water
EQUIPMENT
VI.A. Humidifier stand
VII
SAFETY PRECAUTIONS
VII.A. Members of the MAL have been trained extensively in the procedures described in this
SOP.
VIII
DEFINITIONS
VIII.A. Standard Operating Procedure (SOP) – Standard Operating Procedure is a document that
provides instructions for completing a specific task in the lab.
VIII.B. Metabolic Assessment Laboratory (MAL) – The Metabolic Assessment Laboratory is the
laboratory that will use this SOP.
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SOP Number: CP018.00
Title: Preparation of Humidifier Stand
Revision No:
00
IX
Replaces:
N/A
Date in effect:
7/15/2010
Page:
Page 2 of 4
PROCEDURE
IX.A. Description of Humidifier Stand
IX.A.1. The humidifier stands are made of plastic pipe (30 inches high, 15 inches wide
15 inches deep) with a plexi-glass shelf on the top and bottom.
IX.A.2. See Figure 1 below for description.
Humidifier Stand
Figure 1.
IX.B. Cleaning the Humidifier Apparatus
IX.B.1. Wash the humidifier, stand, and shelves with disinfectant cleaning solution.
IX.B.2. Clean the humidifier rotor by removing the four screws from its housing. Refer
to Figure 2 for a description of the apparatus and its components.
Screw
Diffuser Spout
Rotor
Figure 2.
IX.B.3. Remove housing and clean fins as needed. Refer to Figure 3 for a description of
the fins.
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SOP Number: CP018.00
Title: Preparation of Humidifier Stand
Revision No:
00
Replaces:
N/A
Date in effect:
7/15/2010
Page:
Page 3 of 4
Fins
Figure 3.
IX.B.4. Use a clean pipe cleaner to clean diffuser spout.
IX.C. Reassemble and Start the Humidifier
IX.C.1. Place one humidifier on the top shelf and second humidifier on the bottom shelf.
IX.C.2. Fill the humidifier on the bottom shelf with distilled water first being careful to
not fill above the maximum fill line. Then fill the humidifier on the top shelf so
that the stand will not be top heavy. Refer to Figure 4.
Maximum Fill Line
Figure 4.
IX.C.3. Refill the humidifiers every 3 hours.
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 Controlled Copy No._______
SOP Number: CP018.00
Title: Preparation of Humidifier Stand
Revision No:
00
X
Replaces:
N/A
Date in effect:
7/15/2010
Page:
Page 4 of 4
ATTACHMENTS
X.A. For a list of materials and their locations, refer to SOP# CG098.00.
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