Study Questions Amgen Lab 5: Transformation of E. coli
1. What is the purpose of Lab 5?
2. Why is the pARA plasmid now called pARA-R?
3. Define transformation
4. Who was Frederick Griffith?
5. Define transformation efficiency. (thought question)
6. What factors determine transformation efficiency?
7. The recombinant plasmids we prepared are probably_____________.
8. What are “competent” cells?
9. Does transformation occur in nature?
10. How do you prepare “competent” cells in the laboratory? Explain in detail!
11. Why do we need to “heat shock” the bacteria? Explain in detail!
12. Describe the your results for the LB agar, LB/Amp, and LB/Amp/Ara plates?