Supplementary Table 1. Life span statistics.
Each row represents a life span condition. Columns in order from left to right represent: the control genotype, increased dSir2 condition
genotype, sex, mean life span (the increased dSir2 condition mean lifespan / the control mean life span), the percentage increase in mean life
span, the median life span ((the increased dSir2 condition median lifespan / the control median life span), the percentage increase in median life
span, maximum life span (the increased dSir2 condition maximum lifespan / the control mean life span, where maximum life span = mean life
span of last 10% surviving), the percentage increase in maximum life span, the number of flies in the control condition, the number of flies in the
increased dSir2 expression condition, the Chi-squared value, the p-value, and whether the life span was passed every day (ED) or every other day
(EOD). Chi-squared values and p-values were calculated using a log rank test and the survival package in R.
Life Span Information and Statistics
Mean
%
Increase
Median
%
Increase
in Median
Contro
l
Conditio
n
Life Span
-30.228
Life Span
Condition/C
trl
39/53
Life Span
-26.415
# Flies
315
# Flies
206
chisq
205
Control
Tub-W1118
Condition
Tub-Sir2-1
Sex
Males
Life Span
Condition/Ct
rl
38.20/54.75
Tub-W1118
Tub-Sw-W1118etoh
Tub-Sw-W1118etoh
Tub-Sw-Sir2-1etoh
Tub-Sw-Sir2-3etoh
Tub-Sw-Sir2-4etoh
W1118
W1118
W1118
Tub-Sir2-3
Males
59.51/54.75
8.694
59/53
11.321
315
296
Tub-Sir2-3-etoh
Tub-Sw-W1118200RU
Tub-Sw-Sir2-1200RU
Tub-Sw-Sir2-3200RU
Tub-Sw-Sir2-4200RU
Het Sir2-1
Het Sir2-3
Het Sir2-4
Males
69.03/61.77
11.753
68/63
7.937
300
Males
59.99/61.77
-2.882
63/63
0.000
Males
47.82/54.74
-12.642
47/63
Males
69.04/69.03
0.014
Males
Males
Males
Males
66.19/67.37
65.38/68.72
77.28/68.72
77.99/68.72
Elav-Sw-Sir2-3
Elav-Sw-Sir2-3500RU
Males
Males
Female
s
Elav-Sw-W1118
Elav-Sw-Sir2-3
etoh
Tub-Sw-Myc2etoh
Tub-Sw-Myc2-100
in Mean
ED/EOD
EOD
20.7
p-value
0
0.000005
28
282
56.9
4.49E-14
ED
300
294
1.6
0.199
ED
-25.397
293
266
51
9.37E-13
ED
70/68
2.941
282
276
0.2
ED
-1.752
-4.860
12.456
13.490
66/67
67/70
82/70
82/70
-1.493
-4.286
17.143
17.143
289
279
279
279
297
301
296
290
17.3
10.6
87.9
75.8
57.36/54.11
6.006
57/55
3.636
358
281
17.5
0.63
0.000032
7
0.00112
0
0
0.000028
4
EOD
64.01/57.36
11.593
65/57
14.035
281
293
52.5
4.4E-13
EOD
62.3/65.4
-4.740
64/66
-3.030
252
264
4.1
0.0433
EOD
EOD
ED
ED
ED
ED
Tub-Sw-Myc2etoh
Tub-Sw-Myc2etoh
Tub-Sw-Myc9etoh
Tub-Sw-Myc9etoh
Tub-Sw-Myc9etoh
Tub-Sw-Myc2-200
Tub-Sw-Myc2-500
Tub-Sw-Myc9-100
Tub-Sw-Myc9-200
Tub-Sw-Myc9-500
Female
s
Female
s
Female
s
Female
s
Female
s
Life Span Information and Statistics
(Continued)
Control
Tub-Sw-Myc2etoh
Tub-Sw-Myc2etoh
Tub-Sw-Myc2etoh
Tub-Sw-Myc9etoh
Tub-Sw-Myc9etoh
Tub-Sw-Myc9etoh
70.3/65.4
7.492
72/66
9.091
252
245
14.6
0.000134
EOD
73.3/65.4
12.080
78/66
18.182
252
246
52.8
3.78E-13
EOD
68.14/69.14
-1.446
74/76
-2.632
247
238
0.7
0.415
EOD
76.9/69.14
11.224
80/76
5.263
247
233
31.4
2.15E-08
EOD
78.1/69.14
12.959
82/76
7.895
247
251
51.1
8.87E-13
EOD
Mean
%
Increase
Median
% Increase
in Median
Contro
l
Conditio
n
Life Span
# Flies
# Flies
chisq
p-value
ED/EO
D
Condition
Sex
Life Span
Condition/Ct
rl
Tub-Sw-Myc2-100
Males
60.4/57.36
5.300
64/64
0.000
228
226
0.3
0.606
EOD
Tub-Sw-Myc2-200
Males
61.04/57.36
6.416
64/64
0.000
228
252
0.1
0.7
EOD
Tub-Sw-Myc2-500
Males
63.88/57.36
11.367
66/64
3.125
228
239
7.5
0.00626
EOD
Tub-Sw-Myc9-100
Males
62.60/61.78
1.327
68/64
6.250
245
247
7.7
0.00547
EOD
Tub-Sw-Myc9-200
Males
67.57/61.78
9.372
70/64
9.375
245
230
20.3
0.00000657
EOD
Tub-Sw-Myc9-500
Males
Female
s
Female
s
Female
s
Female
66.06/61.78
6.928
70/64
9.375
245
240
8.7
0.00321
EOD
48.56/62.49
-22.292
49/61
-19.672
349
200
94.3
0
EOD
66.55/62.49
6.497
67/61
9.836
349
289
6.9
0.00881
EOD
78.96/77.53
78.13/77.53
1.844
0.774
80/80
79/80
0.000
-1.250
297
297
282
304
0.1
4.6
0.805
0.0317
ED
ED
Tub-W1118
Tub-Sir2-1
Tub-W1118
Tub-Sw-W1118etoh
Tub-Sw-W1118-
Tub-Sir2-3
Tub-Sir2-3-etoh
Tub-Sw-W1118-
in Mean
Life Span
Life Span
Condition/Ct
rl
etoh
Tub-Sw-Sir2-1etoh
Tub-Sw-Sir2-3etoh
Tub-Sw-Sir2-4etoh
200RU
Tub-Sw-Sir2-1200RU
Tub-Sw-Sir2-3200RU
Tub-Sw-Sir2-4200RU
W1118
Het Sir2-1
W1118
Het Sir2-3
W1118
Het Sir2-4
Elav-Sw-W1118
Elav-Sw-Sir2-3
etoh
Elav-Sw-Sir2-3
Elav-Sw-Sir2-3500RU
s
Female
s
Female
s
Female
s
Female
s
Female
s
Female
s
Female
s
Female
s
61.25/69.55
-11.934
62/71
-12.676
291
271
102
0
ED
82.74/78.96
4.787
85/80
6.250
282
285
48.2
3.76E-12
ED
71.54/70.50
1.475
72/71
1.408
301
301
0.8
ED
70.50/61.53
14.578
75/64
17.188
310
297
28
0.381
0.00000011
9
73.06/61.53
18.739
79/64
23.438
310
298
73.1
0
ED
68.23/61.53
10.889
72/64
12.500
310
310
11
0.000929
ED
68.53/66.37
3.254
69/69
0.000
366
293
4.7
0.0309
EOD
70.74/69.18
2.255
71/69
2.899
293
297
0.3
0.564
EOD
Supplementary Table 2. dSir2 mRNA Expression Levels
dSir2 expression levels in reported conditions. Each row represents a life span condition. Columns in order from left to right represent: the
control genotype, the increased dSir2 expression condition genotype, the dSir2 mRNA level as determined by qPCR, and the p-value as
determined by student’s t-test.
Control
Over expression condition
dSir2 mRNA Level
p-value
Tub-W1118
Tub-Sir2-1
43.21
<0.0001
Tub-W1118
Tub-Sir2-3
11.19
<0.0001
Tub-Sw-W1118-etoh
Tub-Sir2-3-etoh
1.30
0.0078
Tub-Sw-Sir2-1-etoh
Tub-Sw-Sir2-1-200RU
55.69
<0.0001
Tub-Sw-Sir2-3-etoh
Tub-Sw-Sir2-3-200RU
11.65
<0.0001
Tub-Sw-Sir2-4-etoh
Tub-Sw-Sir2-4-200RU
15.30
0.00021
W1118
Het Sir2-1
1.29
0.00278
W1118
Het Sir2-3
1.48
0.00224
W1118
Het Sir2-4
1.88
<0.0001
ED
Elav-Sw-W1118
Elav-Sw-Sir2-3
1.45
0.00234
Elav-Sw-Sir2-3 etoh
Elav-Sw-Sir2-3-500RU
4.98
<0.0001
Tub-Sw-Myc2-etoh
Tub-Sw-Myc2-100
1.87
0.00332
Tub-Sw-Myc2-etoh
Tub-Sw-Myc2-200
3.78
<0.0001
Tub-Sw-Myc2-etoh
Tub-Sw-Myc2-500
4.94
0.00029
Tub-Sw-Myc9-etoh
Tub-Sw-Myc9-100
3.12
0.01865
Tub-Sw-Myc9-etoh
Tub-Sw-Myc9-200
4.54
0.00068
Tub-Sw-Myc9-etoh
Tub-Sw-Myc9-500
7.54
<0.0001
Supplementary Table 3. dSir2 and DNAJ-H mRNA Expression Levels for Figures 3 and S2
dnaJ-H and puc expression levels in reported conditions. Each row represents an experimental condition. Columns represent, in order from left
to right: The control genotype, the increased dSir2 or GFP expression condition genotype, the dSir2 level as determined by qPCR, the p-value for
dSir2 qPCR as determined by student’s t-test, the dnaJ-H level as determined by qPCR, the p-value for dnaJ-H levels as determined by student’s
t-test, the puc level as determined by qPCR, the p-value for puc levels as determined by student’s t-test.
Over-expression Condition
Elav-Gal4-EP2300
Control
Elav-Gal4-W1118
dSir2 Level
3.41
dSir2 pvalue
<0.0001
DNAJ-H
Level
0.745
DNAJ-H pvalue
0.34245
puc level
0.981
puc pvalue
0.18223
Elav-Gal4-Sir2-1
Elav-Gal4-W1118
12.72
0.00033
2.333
0.00025
2.456
0.00249
Elav-Gal4-Sir2-3
Elav-Gal4-W1118
6.196
0.00348
1.593
0.00377
1.862
0.01736
Elav-Gal4-Sir2-4
Elav-Gal4-W1118
7.136
0.00189
1.812
0.00512
2.303
0.0088
Elav-Switch-Sir2-1, 500 uM RU
Elav-Switch-Sir2-1, etoh
32.946
<0.0001
Elav-Switch-Sir2-3, 500 uM RU
Elav-Switch-Sir2-3, etoh
4.998
0.00036
Elav-Switch-Min UAS-Sir2, 500 uM RU
Elav-Switch-Min UAS-Sir2, etoh
49.607
<0.0001
Tub-Switch-Min UAS-Sir2, 200 uM RU
Tub-Switch-Min UAS-Sir2, etoh
24.825
<0.0001
2.863
<0.0001
Tub-Switch-Min UAS-Sir2, 50 uM RU
Tub-Switch-Min UAS-Sir2, etoh
3.233
0.00358
0.414
<0.0001
Da-Gal4-UAS-GFP
Da-Gal4-YW
2.681
0.0021
Supplementary Table 4. Primers used for qPCR amplification.
Each row represents a primer pair used in qPCR, columns represent, in order from left to right: Transcript being measured, the forward primer
sequence, and the reverse primer sequence.
Gene
Forward:
Reverse:
GAPDH
GACGAAATCAAGGCTAAGGTCG
AATGGGTGTCGCTGAAGAAGTC
dSir2
GTCGAGAACGGCATCATGAAG
GGAGCCGATCACGATCAGTAG
DNAJ-H
GGTCTCAGCCCCTTCGATAC
AGGGACTGCACTTCTTGTCG
puc
GCCACATCAGAACATCAAGC
CCGTTTTCCGTGCATCTT